Comparing GFF2318 FitnessBrowser__Phaeo:GFF2318 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
P23533 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Staphylococcus carnosus (strain TM300) (see paper)
34% identity, 73% coverage: 183:723/746 of query aligns to 5:549/573 of P23533
2wqdA Crystal structure of enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state (see paper)
34% identity, 70% coverage: 182:705/746 of query aligns to 3:531/570 of 2wqdA
P08839 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 60% coverage: 272:721/746 of query aligns to 96:546/575 of P08839
2hwgA Structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system (see paper)
35% identity, 60% coverage: 272:721/746 of query aligns to 95:545/572 of 2hwgA
Sites not aligning to the query:
2xz7A Crystal structure of the phosphoenolpyruvate-binding domain of enzyme i in complex with phosphoenolpyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
38% identity, 40% coverage: 431:732/746 of query aligns to 8:309/324 of 2xz7A
2xz9A Crystal structure from the phosphoenolpyruvate-binding domain of enzyme i in complex with pyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
38% identity, 40% coverage: 431:732/746 of query aligns to 1:302/317 of 2xz9A
5lu4A C4-type pyruvate phosphate dikinase: conformational intermediate of central domain in the swiveling mechanism (see paper)
26% identity, 48% coverage: 353:711/746 of query aligns to 445:845/850 of 5lu4A
Sites not aligning to the query:
5jvjB C4-type pyruvate phosphate dikinase: different conformational states of the nucleotide binding domain in the dimer (see paper)
24% identity, 48% coverage: 353:711/746 of query aligns to 372:790/797 of 5jvjB
Sites not aligning to the query:
O23404 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 48% coverage: 353:711/746 of query aligns to 534:955/963 of O23404
5jvlA C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
24% identity, 48% coverage: 353:711/746 of query aligns to 445:867/874 of 5jvlA
Sites not aligning to the query:
Q39735 Pyruvate, phosphate dikinase, chloroplastic; Cold-sensitive pyruvate, orthophosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) (see 2 papers)
24% identity, 48% coverage: 353:711/746 of query aligns to 524:946/953 of Q39735
Sites not aligning to the query:
5jvlB C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
24% identity, 47% coverage: 363:711/746 of query aligns to 124:513/520 of 5jvlB
Sites not aligning to the query:
P11155 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Zea mays (Maize) (see 7 papers)
25% identity, 48% coverage: 353:711/746 of query aligns to 518:940/947 of P11155
Sites not aligning to the query:
1vbgA Pyruvate phosphate dikinase from maize (see paper)
25% identity, 48% coverage: 353:711/746 of query aligns to 445:867/874 of 1vbgA
Sites not aligning to the query:
1kc7A Pyruvate phosphate dikinase with bound mg-phosphonopyruvate (see paper)
24% identity, 47% coverage: 353:705/746 of query aligns to 443:858/872 of 1kc7A
Sites not aligning to the query:
P22983 Pyruvate, phosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Clostridium symbiosum (Bacteroides symbiosus) (see 4 papers)
24% identity, 47% coverage: 353:705/746 of query aligns to 444:859/874 of P22983
Sites not aligning to the query:
1vbhA Pyruvate phosphate dikinase with bound mg-pep from maize (see paper)
25% identity, 47% coverage: 363:711/746 of query aligns to 443:855/862 of 1vbhA
Sites not aligning to the query:
Q02KR1 Phosphoenolpyruvate synthase; PEP synthase; Pyruvate, water dikinase; EC 2.7.9.2 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
26% identity, 47% coverage: 354:705/746 of query aligns to 408:777/791 of Q02KR1
>GFF2318 FitnessBrowser__Phaeo:GFF2318
MVKTTESESRNLLMRLREAMAGDDAGQARLDKITQLIADSMQSEVCSVYLFRDDETLELC
ATQGLNVESVHQTRMRIGEGLVGRVARYGKVINTPDAPNAKGFRYMPETGEERFSSFLGV
PIQRLGEMLGVLVVQSKEGREFSSDAVYALEVVAMVIAEMTELGAFVGEGAALSPLHQQP
VLMRGTIAQEGAVEGHVWLHEPRVVVTNPIADDPHRELERLHEAVEELRVGVDKMLEVTQ
TGDKEQLQVLEAYRMFANSKGWMRRMEEDIGRGLSAEAAVEKEQSQARARMGQVQDAYLR
ERLSDLDDLSNRLLRILTGQGSETGAELPEDPVLIARNIGPGELLEYGRNLRGIVLEEGS
VGSHAAIIARALAIPLVVHTKRITTEALNGDHIMVDGEQGVVHLRPDDTVVSAFRDKIAM
QAKAQERYASIRDKQALTRDGRRVHLLMNAGLMADLPSLENSGAEGVGLFRTELQFLVRN
QMPKRSELVALYQRVLDAAGGKRVVFRTLDIGSDKVLPYMKPTDEPNPALGWRAIRVGLD
KPGVMRMQLQALIRAANGRPLTVMFPFVAQFEEFRDAKAEVEKTLERERRLGHALPEKLE
VGAMLETPSLAFAPQKFFDEVEFLSIGGNDLKQFFFAADRENERVRKRYDTLNVSYLSFI
AQIVERCEKSGTPLSFCGEDAGRPIEAVCLAAMGLRVLSMRPASVGPVKSLLMRVDLNDI
RKIIADARHRGEQTVRPAVMQYLREL
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory