SitesBLAST
Comparing GFF2329 FitnessBrowser__WCS417:GFF2329 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
61% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt5A
- active site: K97 (= K97), H176 (= H176)
- binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K97), H155 (= H155), H176 (= H176), Y235 (= Y235)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), E96 (= E96), K97 (= K97), Y280 (= Y280)
3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
61% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt4A
- active site: K97 (= K97), H176 (= H176)
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (= H155), H176 (= H176), Y235 (= Y235), W272 (= W272)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), E96 (= E96), K97 (= K97), H176 (= H176)
3nt2B Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
61% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt2B
- active site: K97 (= K97), H176 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), A78 (≠ S78), K97 (= K97), W272 (= W272), Y280 (= Y280)
3nt2A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
61% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt2A
- active site: K97 (= K97), H176 (= H176)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), A78 (≠ S78), H79 (= H79), E96 (= E96), K97 (= K97), H176 (= H176), Y280 (= Y280)
4l8vA Crystal structure of a12k/d35s mutant myo-inositol dehydrogenase from bacillus subtilis with bound cofactor NADP (see paper)
61% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 4l8vA
- active site: K97 (= K97), H176 (= H176)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ A12), I13 (= I13), S74 (= S74), W75 (= W75), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), W272 (= W272), Y280 (= Y280)
3ec7A Crystal structure of putative dehydrogenase from salmonella typhimurium lt2
51% identity, 100% coverage: 1:335/336 of query aligns to 1:335/336 of 3ec7A
- active site: K97 (= K97), H176 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), M12 (≠ A12), I13 (= I13), D35 (= D35), I36 (= I36), R40 (≠ Q40), T73 (= T73), A74 (≠ S74), S75 (≠ W75), N76 (≠ G76), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), W271 (= W272), Y279 (= Y280)
4mjlD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD and d-chiro-inositol
48% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mjlD
- active site: K97 (= K97)
- binding (1r,2r,3s,4s,5s,6s)-cyclohexane-1,2,3,4,5,6-hexol: K97 (= K97), H155 (= H155), N157 (= N157), D172 (= D172), T173 (= T173), H176 (= H176), Y236 (= Y235)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), V73 (≠ T73), S74 (= S74), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), D172 (= D172), W274 (= W272), Y282 (= Y280)
4mioD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
48% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mioD
- active site: K97 (= K97)
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: K97 (= K97), H155 (= H155), N157 (= N157), D172 (= D172), T173 (= T173), H176 (= H176), Y236 (= Y235)
- binding 1,4-dihydronicotinamide adenine dinucleotide: A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), S74 (= S74), A78 (≠ S78), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), D172 (= D172), W274 (= W272), Y282 (= Y280)
4mioA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
48% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mioA
- active site: K97 (= K97)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), S74 (= S74), F75 (≠ W75), H79 (= H79), E96 (= E96), K97 (= K97), W274 (= W272), Y282 (= Y280)
4minA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD
48% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4minA
- active site: K97 (= K97)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), V73 (≠ T73), S74 (= S74), F75 (≠ W75), H79 (= H79), E96 (= E96), K97 (= K97), Y282 (= Y280)
4n54A Crystal structure of scyllo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD(h) and scyllo-inositol
26% identity, 97% coverage: 2:327/336 of query aligns to 2:339/340 of 4n54A
- active site: K96 (= K97), H183 (= H176)
- binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: R12 (≠ A12), K96 (= K97), D156 (≠ N157), D179 (= D172), M180 (≠ T173), H183 (= H176), R238 (≠ K219), Y244 (= Y235)
- binding 1,4-dihydronicotinamide adenine dinucleotide: V8 (≠ I8), G9 (= G9), L10 (≠ T10), G11 (= G11), R12 (≠ A12), L13 (≠ I13), S35 (≠ D35), V36 (≠ I36), E40 (≠ N37), S73 (= S74), P74 (≠ W75), F77 (≠ S78), H78 (= H79), E95 (= E96), K96 (= K97), M125 (= M126), F167 (≠ M168), F284 (≠ W272)
3ceaA Crystal structure of myo-inositol 2-dehydrogenase (np_786804.1) from lactobacillus plantarum at 2.40 a resolution
25% identity, 97% coverage: 3:328/336 of query aligns to 5:342/342 of 3ceaA
- active site: K98 (= K97), H185 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G9), G13 (= G11), R14 (≠ A12), L15 (≠ I13), L38 (≠ I36), Q42 (= Q40), V74 (≠ T73), A75 (≠ S74), P76 (≠ W75), T77 (≠ G76), F79 (≠ S78), H80 (= H79), M83 (≠ F82), E97 (= E96), K98 (= K97), M127 (= M126), F169 (≠ M168), H185 (= H176), F286 (≠ W272)
7xr9A Crystal structure of dgpa with glucose (see paper)
32% identity, 48% coverage: 35:196/336 of query aligns to 29:200/344 of 7xr9A
- binding beta-D-glucopyranose: K91 (= K97), R150 (≠ H155), W157 (≠ V160), F160 (vs. gap), D173 (= D172), H177 (= H176)
- binding nicotinamide-adenine-dinucleotide: D51 (= D57), Y52 (≠ G58), C67 (≠ T73), T68 (≠ S74), P69 (≠ W75), H73 (= H79), E90 (= E96), K91 (= K97), P92 (= P98), Q119 (≠ M126), V159 (vs. gap), F160 (vs. gap), Q166 (≠ K165)
Sites not aligning to the query:
2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
31% identity, 55% coverage: 4:188/336 of query aligns to 2:191/332 of 2glxA
- active site: K93 (= K97), H179 (= H176)
- binding acetate ion: K93 (= K97), H179 (= H176)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G9), A8 (≠ T10), S9 (≠ G11), T10 (≠ A12), I11 (= I13), S32 (≠ D35), T33 (≠ I36), R37 (≠ Q40), S69 (≠ T73), T70 (≠ S74), N72 (≠ G76), H75 (= H79), E92 (= E96), K93 (= K97), H121 (≠ F125), W161 (≠ Y164), R162 (≠ K165)
Sites not aligning to the query:
Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
31% identity, 55% coverage: 4:188/336 of query aligns to 3:192/333 of Q2I8V6
- ASTI 9:12 (≠ TGAI 10:13) binding
- S10 (≠ G11) mutation to G: Almost no effect.
- A13 (≠ G14) mutation to G: Can use NAD as cosubstrate as well as NADP.
- S33 (≠ D35) mutation to D: No activity.
- ST 33:34 (≠ DI 35:36) binding
- R38 (≠ Q40) binding
- TTNELH 71:76 (≠ SWGPSH 74:79) binding
- EK 93:94 (= EK 96:97) binding
- K94 (= K97) mutation to G: Less than 1% remaining activity.
- N120 (≠ V123) binding
- WR 162:163 (≠ YK 164:165) binding
- D176 (= D172) mutation to A: Less than 1% remaining activity.
- H180 (= H176) mutation to A: Less than 2% remaining activity.
Sites not aligning to the query:
- 206 G→I: No effect.
- 283 binding
7xreC Crystal structure of dgpa
32% identity, 48% coverage: 28:187/336 of query aligns to 31:198/363 of 7xreC
- binding nicotinamide-adenine-dinucleotide: D38 (= D35), I39 (= I36), T78 (≠ S74), P79 (≠ W75), N80 (≠ G76), H83 (= H79), E100 (= E96), K101 (= K97), P102 (= P98), F170 (vs. gap), Q176 (≠ K165), H187 (= H176)
Sites not aligning to the query:
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
26% identity, 56% coverage: 7:193/336 of query aligns to 7:205/340 of 1evjA
- active site: K100 (= K97), Y188 (≠ H176)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), L10 (≠ T10), G11 (= G11), K12 (≠ A12), Y13 (≠ I13), D35 (= D35), L77 (≠ S74), P78 (≠ W75), N79 (≠ G76), H82 (= H79), E99 (= E96), K100 (= K97), R128 (≠ M126), W170 (≠ E162), R171 (≠ N163), Y188 (≠ H176)
Sites not aligning to the query:
3e18A Crystal structure of NAD-binding protein from listeria innocua
27% identity, 77% coverage: 4:261/336 of query aligns to 5:277/348 of 3e18A
- active site: K94 (= K97), H178 (= H176)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), G13 (≠ A12), M14 (≠ I13), F34 (≠ T34), D35 (= D35), I36 (= I36), K40 (= K43), A70 (≠ T73), T71 (≠ S74), P72 (≠ W75), N73 (≠ G76), H76 (= H79), E93 (= E96), K94 (= K97), N122 (≠ M126), W161 (≠ G161), R162 (≠ E162), H178 (= H176)
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
26% identity, 56% coverage: 7:193/336 of query aligns to 38:236/383 of 1h6dA
- active site: K131 (= K97), Y219 (≠ H176)
- binding glycerol: K131 (= K97), R202 (≠ N163), D215 (= D172), Y219 (≠ H176)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G9), L41 (≠ T10), G42 (= G11), K43 (≠ A12), Y44 (≠ I13), S66 (≠ D35), G67 (≠ I36), K71 (≠ Q40), Y89 (= Y55), I107 (≠ T73), L108 (≠ S74), P109 (≠ W75), N110 (≠ G76), H113 (= H79), E130 (= E96), K131 (= K97), R159 (≠ M126), A198 (≠ T159), W201 (≠ E162), R202 (≠ N163), Y219 (≠ H176)
Sites not aligning to the query:
4koaA Crystal structure analysis of 1,5-anhydro-d-fructose reductase from sinorhizobium meliloti (see paper)
30% identity, 55% coverage: 3:188/336 of query aligns to 2:192/333 of 4koaA
- active site: K94 (= K97), H180 (= H176)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), A9 (≠ T10), S10 (≠ G11), T11 (≠ A12), I12 (= I13), S33 (≠ D35), S34 (≠ I36), R38 (≠ Q40), T71 (≠ S74), N73 (≠ G76), H76 (= H79), K94 (= K97), Q160 (≠ E162)
Query Sequence
>GFF2329 FitnessBrowser__WCS417:GFF2329
MSLKLGVIGTGAIGRDHIRRCSQTLLNSQVVAVTDINLEQAAKVVADLNISAEVYADGHA
LIHSPEVEAVLVTSWGPSHEEFVLAAIAAGKPVFCEKPLAVTAEGCRKIVDAEVAFGKRL
VQVGFMRPYDDGYRALKAVIDSGQIGEPLMLHCAHRNPTVGENYKTDMAITDTLIHELDV
LRWLLNDDYVSVQVVFPRKSSKALAHLRDPQIVLLETAKGTRIDVEVFVNCQYGYDIQCE
VVGETGIAKLPEPSQVQLRSGAKLSNAILMDWKDRFIGAYDVELQAFIDSVRAGQVGGPS
AWDGFAAAVAADACIEAQNSEQIVKVSLPDRPHFYG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory