SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2360 FitnessBrowser__WCS417:GFF2360 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

Q9CIV7 PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
36% identity, 88% coverage: 21:209/215 of query aligns to 18:191/192 of Q9CIV7

query
sites
Q9CIV7

I

I

V

Q

A

E

N

N

R

K

E

A

Y

Y

L

L

S

S

E

E

I

L

D
|
D

G

G

A

P

I

I

G

G

D
|
D

G

G

D
|
D

H
|
H

G
|
G

I
x
A

N
|
N

M

M

A

A

K

R

G

G

F

M

A

S

H

E

C

T

G

M

R

K

T

A

L

L

E

E

G

V

R

S

Q

N

L

F

-

G

T

N

L

V

A

S

E

E

A

I

L

F

D

K

E

K

L

V

T

A

L

M

S

T

L

L

M

M

E

S

G

K

I

V

G

G

S
x
A

M
x
S

G

G

P

P

L

L

Y

Y

G

G

S

S

L

A

F

F

I

L

G

A

M

M

A

S

D

K

E

T

V

A

R

-

N

-

S

-

D

-

I

I

D

E

A

T

A

L

T

D

F

T

A

S

H

E

L

L

L

I

R

Y

G

A

G

G

L

L

T

E

S

A

L

I

Q

Q

D

K

I
x
R

T
x
G

E

K

A

A

G

Q

V

V

G

G

D

E

K

K

C

T

L
x
M

M

V

D

D

T

I

L

W

I

-

P

-

A

-

V

-

E

S

A

A

F

F

E

L

Q

N

A

D

H

L

A

Q

T

T

G

D

A

S

S

A

F

S

S

K

E

D

A

N

L

L

D

E

A

K

M

V

K

-

A

V

A

K

A

A

S

S

Q

A

G

G

R

-

D

-

S

-

T

-

K

-

D

-

L

L

V

L

A

A

K

T

I

K

G

G

R
|
R

A

A

S

S

R
x
Y

L

L

G

G

E

E

R

R

S

S

L

I

G

G

V

H

L

I

D
|
D

A
x
P

G
|
G

A

T

V

Q

S

S

C

S

C

A

L

Y

I

L

L

F

T

E

R

T

L

L

A

L

D

E

S

V

V

V

D29 (= D32) binding
D34 (= D37) binding
D36 (= D39) binding
HGAN 37:40 (≠ HGIN 40:43) binding
AS 78:79 (≠ SM 80:81) binding
R114 (≠ I121) mutation to A: Reduces activity 3-fold.; mutation to E: Reduces activity 100-fold.
G115 (≠ T122) binding
M124 (≠ L131) binding
R161 (= R179) mutation to A: Loss of activity.
Y164 (≠ R182) mutation to A: Reduces activity about 20-fold.
DPG 174:176 (≠ DAG 192:194) binding

3cr3A Structure of a transient complex between dha-kinase subunits dham and dhal from lactococcus lactis (see paper)
36% identity, 88% coverage: 21:209/215 of query aligns to 18:191/192 of 3cr3A

query
sites
3cr3A

I

I

V

Q

A

E

N

N

R

K

E

A

Y

Y

L

L

S

S

E

E

I

L

D
|
D

G

G

A

P

I

I

G

G

D
|
D

G

G

D
|
D

H
|
H

G

G

I

A

N
|
N

M

M

A

A

K

R

G

G

F

M

A

S

H

E

C

T

G

M

R

K

T

A

L

L

E

E

G

V

R

S

Q

N

L

F

-

G

T

N

L

V

A

S

E

E

A

I

L

F

D

K

E

K

L

V

T

A

L

M

S

T

L

L

M

M

E

S

G

K

I

V

G

G

G
|
G

S
x
A

M
x
S

G

G

P

P

L
|
L

Y

Y

G

G

S

S

L

A

F

F

I

L

G

A

M

M

A

S

D

K

E

T

V

A

R

-

N

-

S

-

D

-

I

I

D

E

A

T

A

L

T

D

F

T

A

S

H

E

L

L

L

I

R

Y

G

A

G

G

L

L

T

E

S

A

L

I

Q

Q

D

K

I

R

T
x
G

E

K

A
|
A

G

Q

V

V

G

G

D

E

K

K

C
x
T

L

M

M

V

D

D

T

I

L

W

I

-

P

-

A

-

V

-

E

S

A

A

F

F

E

L

Q

N

A

D

H

L

A

Q

T

T

G

D

A

S

S

A

F

S

S

K

E

D

A

N

L

L

D

E

A

K

M

V

K

-

A

V

A

K

A

A

S

S

Q

A

G

G

R

-

D

-

S

-

T

-

K

-

D

-

L

L

V

L

A

A

K

T

I

K

G

G

R

R

A

A

S

S

R

Y

L

L

G

G

E

E

R

R

S

S

L

I

G

G

V

H

L

I

D
|
D

A
x
P

G
|
G

A

T

V

Q

S

S

C

S

C

A

L

Y

I

L

L

F

T

E

R

T

L

L

A

L

D

E

S

V

V

V

binding adenosine-5'-diphosphate: D29 (= D32), D34 (= D37), D36 (= D39), H37 (= H40), N40 (= N43), G77 (= G79), A78 (≠ S80), S79 (≠ M81), L82 (= L84), G115 (≠ T122), A117 (= A124), T123 (≠ C130), D174 (= D192), P175 (≠ A193), G176 (= G194)
binding magnesium ion: D29 (= D32), D34 (= D37), D34 (= D37), D36 (= D39), D36 (= D39)

4lrzA Crystal structure of the e.Coli dhar(n)-dhal complex (see paper)
34% identity, 84% coverage: 26:206/215 of query aligns to 25:206/211 of 4lrzA

query
sites
4lrzA

E

E

Y

Y

L

L

S

T

E

G

I

L

D
|
D

G

R

A

E

I

I

G

G

D
|
D

G

A

D
|
D

H
|
H

G

G

I

L

N

N

M

M

A

N

K

R

G

G

F

F

A

S

H

K

C

V

G

V

R

E

T

K

L

L

E

P

G

A

-

I

R

A

Q

D

L

K

T

D

L

I

A

G

E

F

A

I

L

L

D

K

E

N

L

T

T

G

L

M

S

T

L

L

M

L

E

S

G

S

I

V

G

G

G
|
G

S
x
A

M
x
S

G

G

P

P

L
|
L

Y

F

G

G

S

T

L

F

F

F

I

I

G

R

M

A

A

A

D

Q

E

A

V

T

R

Q

N

A

S

R

D

Q

D

S

I

L

D

T

A

L

A

E

T

E

F

L

A

Y

H

Q

L

M

L

F

R

R

G

D

G

G

L

A

T

D

S

G

L

V

Q

I

D

S

I

R

T
x
G

E

K

A

A

G

E

V

P

G

G

D

D

K

K

C
x
T

L
x
M

M

C

D

D

T

V

L

W

I

V

P

P

A

V

V

V

E

E

A

S

F

L

E

R

Q

Q

A

S

H

S

A

E

T

Q

G

N

A

L

S

S

F

V

S

P

E

V

A

A

L

L

D

E

A

A

M

A

K

S

A

S

A

I

A

A

S

E

Q

S

G

A

R

A

D

Q

S

S

T

T

K

I

D

T

L

M

V

Q

A

A

K

R

I

K

G
|
G

R

R

A

A

S

S

R

Y

L

L

G

G

E

E

R

R

S

S

L

I

G

G

V

H

L

Q

D
|
D

A
x
P

G
|
G

A

A

V

T

S

S

C

V

C

M

L

F

I

M

L

M

T

Q

R

M

L

L

A

A

binding adenosine-5'-diphosphate: D31 (= D32), D36 (= D37), D38 (= D39), H39 (= H40), G79 (= G79), A80 (≠ S80), S81 (≠ M81), L84 (= L84), G122 (≠ T122), T130 (≠ C130), M131 (≠ L131), G178 (= G178), D192 (= D192), P193 (≠ A193), G194 (= G194)
binding magnesium ion: D31 (= D32), D36 (= D37), D36 (= D37), D38 (= D39), D38 (= D39)

P76014 PEP-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL; EC 2.7.1.121 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 84% coverage: 26:206/215 of query aligns to 24:205/210 of P76014

query
sites
P76014

E

E

Y

Y

L

L

S

T

E

G

I

L

D
|
D

G

R

A

E

I

I

G

G

D
|
D

G

A

D
|
D

H
|
H

G
|
G

I
x
L

N
|
N

M

M

A

N

K

R

G

G

F

F

A

S

H

K

C

V

G

V

R

E

T

K

L

L

E

P

G

A

-

I

R

A

Q

D

L

K

T

D

L

I

A

G

E

F

A

I

L

L

D

K

E

N

L

T

T

G

L

M

S

T

L

L

M

L

E

S

G

S

I

V

G

G

S
x
A

M
x
S

G

G

P

P

L

L

Y

F

G

G

S

T

L

F

F

F

I

I

G

R

M

A

A

A

D

Q

E

A

V

T

R

Q

N

A

S

R

D

Q

D

S

I

L

D

T

A

L

A

E

T

E

F

L

A

Y

H

Q

L

M

L

F

R

R

G

D

G

G

L

A

T

D

S

G

L

V

Q

I

D

S

I

R

T
x
G

E

K

A

A

G

E

V

P

G

G

D

D

K

K

C

T

L
x
M

M

C

D

D

T

V

L

W

I

V

P

P

A

V

V

V

E

E

A

S

F

L

E

R

Q

Q

A

S

H

S

A

E

T

Q

G

N

A

L

S

S

F

V

S

P

E

V

A

A

L

L

D

E

A

A

M

A

K

S

A

S

A

I

A

A

S

E

Q

S

G

A

R

A

D

Q

S

S

T

T

K

I

D

T

L

M

V

Q

A

A

K

R

I

K

G

G

R
|
R

A

A

S

S

R

Y

L

L

G

G

E

E

R

R

S

S

L

I

G

G

V

H

L

Q

D
|
D

A
x
P

G
|
G

A

A

V

T

S

S

C

V

C

M

L

F

I

M

L

M

T

Q

R

M

L

L

A

A

D30 (= D32) binding
D35 (= D37) binding
D37 (= D39) binding
HGLN 38:41 (≠ HGIN 40:43) binding
AS 79:80 (≠ SM 80:81) binding
G121 (≠ T122) binding
M130 (≠ L131) binding
R178 (= R179) binding
DPG 191:193 (≠ DAG 192:194) binding

Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human) (see 4 papers)
28% identity, 85% coverage: 13:194/215 of query aligns to 377:558/575 of Q3LXA3

query
sites
Q3LXA3

I

V

V

L

S

E

D

R

L

V

V

C

S

S

V

T

I

L

V

L

A

G

N

L

R

E

E

E

Y

H

L

L

S

N

E

A

I

L

D

D

G

R

A

A

I

A

G

G

D
|
D

G

G

D
|
D

H
x
C

G

G

I

T

N

T

M

H

A

S

K

R

G

A

F

A

A

R

H

A

C

I

G

Q

R

E

T

W

L

L

-

K

E

E

G

G

R

P

Q

P

-

P

L

A

T

S

L

P

A

A

E

Q

A

L

L

L

D

S

E

K

L

L

T

S

L

V

S

L

L

L

M

L

E

E

G

K

I

M

G

G

G
|
G

S
|
S

M

S

G

G

P

A

L

L

Y

Y

G

G

S

L

L

F

F

L

I

T

G

A

M

A

A

A

D

Q

E

P

V

L

R

K

N

A

S

K

D

T

D

S

I

L

D

P

A

A

A

W

T

S

F

A

A

A

H

-

L

-

L

M

R

D

G

A

G

G

L

L

T

E

S

A

L

M

Q

Q

D

K

I

Y

T

G

E

K

A

A

G

A

V

P

G

G

D

D

K

R

C

T

L

M

M

L

D

D

T

S

L

L

I

W

P

A

A

A

V

G

E

Q

A

E

F

L

E

Q

Q

A

A

W

H

K

A

S

T

P

G

G

A

A

S

D

F

L

S

L

E

Q

A

V

L

L

D

T

A

K

M

A

K

V

A

K

A

S

A

A

S

E

Q

A

G

A

R

A

D

E

S

A

T

T

K

K

D

N

L

M

V

E

A

A

K

G

I

A

G

G

R
|
R

A

A

S

S

R

Y

L

I

G

S

E

S

R

A

S

R

L

L

G

E

V

Q

L

P

D
|
D

A

P

G

G

D401 (= D37) mutation to A: Abolishes both kinase and FMN cyclase activities.
D403 (= D39) mutation to A: Abolishes both kinase and FMN cyclase activities.
C404 (≠ H40) mutation to A: Decreases both kinase and FMN cyclase activities.
G445 (= G79) to S: in TKFCD; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs1590578831
S446 (= S80) mutation to A: Decreases both kinase and FMN cyclase activities.
R543 (= R179) to I: in TKFCD; reduced protein levels in patient cells; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs547013163
D556 (= D192) mutation to A: Abolishes both kinase and FMN cyclase activities.

Sites not aligning to the query:

112 T→A: Highly decreases kinase activity. No effect on FMN cyclase activity.
185 A → T: in dbSNP:rs2260655
204 K→A: Slightly decreases kinase activity. No effect on FMN cyclase activity.
221 H→A: Abolishes kinase activity but not FMN cyclase activity.

Query Sequence

>GFF2360 FitnessBrowser__WCS417:GFF2360
MSQYFSTRDGGAIVSDLVSVIVANREYLSEIDGAIGDGDHGINMAKGFAHCGRTLEGRQL
TLAEALDELTLSLMEGIGGSMGPLYGSLFIGMADEVRNSDDIDAATFAHLLRGGLTSLQD
ITEAGVGDKCLMDTLIPAVEAFEQAHATGASFSEALDAMKAAASQGRDSTKDLVAKIGRA
SRLGERSLGVLDAGAVSCCLILTRLADSVQPRLSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory