SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2361 FitnessBrowser__WCS417:GFF2361 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

Q9CIV8 PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
37% identity, 98% coverage: 3:327/333 of query aligns to 5:329/332 of Q9CIV8

query
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Q9CIV8

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GSGH 55:58 (= GSGH 54:57) binding
K106 (≠ G105) binding
D111 (= D110) binding
H220 (= H219) active site, Tele-hemiaminal-histidine intermediate

P76015 PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 98% coverage: 1:326/333 of query aligns to 1:349/356 of P76015

query
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P76015

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GSGH 53:56 (= GSGH 54:57) binding
H56 (= H57) mutation H->A,N: Shows a moderate decrease in the catalytic efficiency but at least a 40- to 300-fold increase in affinity for dihydroxyacetone.
K104 (≠ G105) binding
D109 (= D110) binding ; mutation D->A,N: Loss of kinase activity.
H218 (= H219) active site, Tele-hemiaminal-histidine intermediate; mutation H->A,K: Loss of kinase activity.

3ct4A Structure of dha-kinase subunit dhak from l. Lactis (see paper)
36% identity, 98% coverage: 3:327/333 of query aligns to 2:316/318 of 3ct4A

query
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3ct4A

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binding Dihydroxyacetone: G51 (= G53), G52 (= G54), H55 (= H57), F77 (= F79), K103 (≠ G105), D108 (= D110), H207 (= H219)

1uoeA Crystal structure of the dihydroxyacetone kinase from e. Coli in complex with glyceraldehyde (see paper)
34% identity, 95% coverage: 10:326/333 of query aligns to 1:329/336 of 1uoeA

query
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1uoeA

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A

L

L

V

V

S

N

G

N

L

L

G

G

A

A

T

T

P

P

V

L

M

S

E

E

L

L

Y

Y

I

G

F

V

Y

Y

A

N

E

R

V

L

E

T

K

T

Q

R

L

C

T

Q

A

Q

K

A

G

G

L

L

K

T

I

I

H

E

R

R

C

N

Y

L

V

I

G

G

N

A

Y

Y

F

C

T

T

S

S

L

L

E

D

M

M

M

T

G

G

V

F

T

S

L

I

T

T

L

L

G

K

L

V

D

D

A

D

E

E

L

T

T

L

T

A

L

L

I

W

D

D

Q

A

P

P

C

V

R

H

S

T

binding glycerol: G43 (= G53), G44 (= G54), H47 (= H57), F69 (= F79), S71 (= S81), Y97 (= Y107), D100 (= D110), H198 (= H219)

1uodA Crystal structure of the dihydroxyacetone kinase from e. Coli in complex with dihydroxyacetone-phosphate (see paper)
34% identity, 95% coverage: 11:326/333 of query aligns to 2:329/336 of 1uodA

query
sites
1uodA

V

V

V

L

E

D

D

E

M

Q

L

L

R

A

G

G

I

L

L

A

V

K

A

A

H

H

P

P

E

S

L

L

R

T

Q

L

Y

H

E

Q

T

-

N

D

P

P

R

V

V

Y

I

V

V

T

K

R

A

A

K

D

P

A

S

P

G

V

P

A

G

G

R

K

V

V

G

A

I

L

V

L

T

S

G

G

G
|
G

S

S

G

G

H
|
H

E

E

P

P

A

M

F

H

L

C

G

G

Y

Y

V

I

G

G

P

Q

G

G

L

M

V

L

D

S

A

G

V

A

A

C

V

P

G

G

E

E

I

I

F
|
F

S
x
T

S

S

P

P

T

T

A

P

K

D

S

K

F

I

F

F

D

E

A

C

F

A

R

M

A

Q

A

V

D

D

H

G

G

G

A

E

G

G

V

V

A

L

C

L

L

I

Y

I

G
x
K

N

N

Y
|
Y

A

T

G
|
G

D
|
D

N

I

M

L

N

N

V

F

K

E

L

T

A

A

M

T

K

E

M

L

A

L

A

H

S

D

K

S

D

G

M

V

R

K

I

V

R

T

T

T

V

V

A

I

N

D

D

D

V

V

A

A

S

V

A

K

P

D

K

S

A

L

E

Y

I

T

A

A

K

G

R

R

G

G

V

V

A

A

G

N

E

T

I

V

F

L

M

I

W

E

K

K

I

L

G

V

G

G

A

A

A

E

Q

R

H

G

Y

D

D

S

L

L

D

D

G

A

V

C

I

A

R

E

V

L

A

G

Q

R

K

K

T

L

V

N

D

N

H

Q

C

G

R

H

S

S

I

I

G

G

I

I

G

A

L

L

T

G

P

A

C

C

T

-

I

-

A

-

V

-

G

-

K

-

P

-

N

-

F

-

Q

-

I

L

P

A

D

D

G

N

Q

E

M

M

E

E

L

F

G

G

I

V

G

G

H

I

H
|
H

G

G

E

E

P

P

G

G

I

I

D

D

V

R

I

R

P

P

I

F

E

S

P

S

A

L

A

D

A

Q

M

T

A

V

E

D

R

E

M

M

L

F

A

D

P

T

I

L

L

L

A

V

D

N

-

G

-

S

-

Y

-

H

-

R

-

T

-

L

-

R

-

F

R

W

D

D

F

Y

S

Q

Q

Q

-

G

-

S

-

W

-

Q

-

E

-

Q

-

T

-

K

-

Q

-

P

-

L

-

Q

-

S

D

G

N

D

S

R

V

V

V

I

V

A

L

L

V

V

S

N

G

N

L

L

G

G

A

A

T

T

P

P

V

L

M

S

E

E

L

L

Y

Y

I

G

F

V

Y

Y

A

N

E

R

V

L

E

T

K

T

Q

R

L

C

T

Q

A

Q

K

A

G

G

L

L

K

T

I

I

H

E

R

R

C

N

Y

L

V

I

G

G

N

A

Y

Y

F

C

T

T

S

S

L

L

E

D

M

M

M

T

G

G

V

F

T

S

L

I

T

T

L

L

G

K

L

V

D

D

A

D

E

E

L

T

T

L

T

A

L

L

I

W

D

D

Q

A

P

P

C

V

R

H

S

T

binding glyceraldehyde-3-phosphate: G43 (= G53), G44 (= G54), H47 (= H57), F69 (= F79), T70 (≠ S80), K95 (≠ G105), Y97 (= Y107), G99 (= G109), D100 (= D110), H198 (= H219)

3pnqD Crystal structure of e.Coli dha kinase dhak (h56n) complex with dha (see paper)
34% identity, 95% coverage: 11:326/333 of query aligns to 2:327/334 of 3pnqD

query
sites
3pnqD

V

V

V

L

E

D

D

E

M

Q

L

L

R

A

G

G

I

L

L

A

V

K

A

A

H

H

P

P

E

S

L

L

R

T

Q

L

Y

H

E

Q

T

-

N

D

P

P

R

V

V

Y

I

V

V

T

K

R

A

A

K

D

P

A

S

P

G

V

P

A

G

G

R

K

V

V

G

A

I

L

V

L

T

S

G

G

G
|
G

S

S

G

G

H

N

E

E

P

P

A

M

F

H

L

C

G

G

Y

Y

V

I

G

G

P

Q

G

G

L

M

V

L

D

S

A

G

V

A

A

C

V

P

G

G

E

E

I

I

F

F

S

T

S
|
S

P

P

T

T

A

P

K

D

S

K

F

I

F

F

D

E

A

C

F

A

R

M

A

Q

A

V

D

D

H

G

G

G

A

E

G

G

V

V

A

L

C

L

L

I

Y

I

G
x
K

N

N

Y
|
Y

A

T

G

G

D
|
D

N

I

M

L

N

N

V

F

K

E

L

T

A

A

M

T

K

E

M

L

A

L

A

H

S

D

K

S

D

G

M

V

R

K

I

V

R

T

T

T

V

V

A

I

N

D

D

D

V

V

A

A

S

V

A

K

P

D

K

S

A

L

E

Y

I

T

A

A

K

G

R

R

G

G

V

V

A

A

G

N

E

T

I

V

F

L

M

I

W

E

K

K

I

L

G

V

G

G

A

A

A

E

Q

R

H

G

Y

D

D

S

L

L

D

D

G

A

V

C

I

A

R

E

V

L

A

G

Q

R

K

K

T

L

V

N

D

N

H

Q

C

G

R

H

S

S

I

I

G

G

I

I

G

A

L

L

T

G

P

-

C

-

T

-

I

-

A

-

V

-

G

-

K

-

P

-

N

-

F

-

Q

-

I

L

P

A

D

D

G

N

Q

E

M

M

E

E

L

F

G

G

I

V

G

G

H

I

H
|
H

G

G

E

E

P

P

G

G

I

I

D

D

V

R

I

R

P

P

I

F

E

S

P

S

A

L

A

D

A

Q

M

T

A

V

E

D

R

E

M

M

L

F

A

D

P

T

I

L

L

L

A

V

D

N

-

G

-

S

-

Y

-

H

-

R

-

T

-

L

-

R

-

F

R

W

D

D

F

Y

S

Q

Q

Q

-

G

-

S

-

W

-

Q

-

E

-

Q

-

T

-

K

-

Q

-

P

-

L

-

Q

-

S

D

G

N

D

S

R

V

V

V

I

V

A

L

L

V

V

S

N

G

N

L

L

G

G

A

A

T

T

P

P

V

L

M

S

E

E

L

L

Y

Y

I

G

F

V

Y

Y

A

N

E

R

V

L

E

T

K

T

Q

R

L

C

T

Q

A

Q

K

A

G

G

L

L

K

T

I

I

H

E

R

R

C

N

Y

L

V

I

G

G

N

A

Y

Y

F

C

T

T

S

S

L

L

E

D

M

M

M

T

G

G

V

F

T

S

L

I

T

T

L

L

G

K

L

V

D

D

A

D

E

E

L

T

T

L

T

A

L

L

I

W

D

D

Q

A

P

P

C

V

R

H

S

T

binding Dihydroxyacetone: G43 (= G53), G44 (= G54), S71 (= S81), K95 (≠ G105), Y97 (= Y107), D100 (= D110), H196 (= H219)

Q3LXA3 Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); EC 2.7.1.28; EC 2.7.1.29; EC 4.6.1.15 from Homo sapiens (Human) (see 4 papers)
32% identity, 96% coverage: 2:321/333 of query aligns to 4:328/575 of Q3LXA3

query
sites
Q3LXA3

N

K

R

K

V

L

I

V

N

N

D

S

P

V

D

A

Q

G

V

C

V

A

E

D

D

D

M

A

L

L

R

A

G

G

I

L

L

V

V

A

A

C

H

N

P

P

E

N

L

L

R

Q

Q

L

Y

L

E

Q

T

G

N

H

P

R

R

V

V

A

I

L

V

R

K

S

A

D

K

L

P

D

S

S

G

L

P

K

G

G

R

R

V

V

G

A

I

L

V

L

T

S

G

G

S

S

G

G

H

H

E

E

P

P

A

A

F

H

L

A

G

G

Y

F

V

I

G

G

P

K

G

G

L

M

V

L

D

T

A

G

V

V

A

I

V

A

G

G

E

A

I

V

F

F

S

T

S

S

P

P

T

A

A

V

K

G

S

S

F

I

F

L

D

A

A

A

F

I

R

R

A

A

A

V

D

A

H

Q

G

A

A

G

G

T

V

V

A

G

C

T

L

L

Y

L

-

I

-

V

G

K

N

N

Y

Y

A
x
T

G

G

D

D

N

R

M

L

N

N

V

F

K

G

L

L

A

A

M

R

K

E

M

Q

A

A

A

R

S

A

K

E

D

G

M

I

R

P

I

V

R

E

T

M

V

V

A

I

N

G

D

D

V

S

A

A

S

F

A

T

P

V

K

L

A

K

E

K

I

-

A

A

K

G

R

R

G

G

V

L

A

C

G

G

E

T

I

V

F

L

M

I

W

H

K

K

I

V

G

A

G

G

A

A

A

L

A

A

A

E

Q

A

H

G

Y

V

D

G

L

L

D

E

G

E

V

I

I

A

R

K

V

Q

A

V

Q

N

K

V

T

V

V
x
A

D

K

H

A

C

M

R

G

S

T

I

L

G

G

I

V

G

S

L

L

T

S

P

S

C

C

T

S

I

V

A

P

A

G

V

-

G

S

K
|
K

P

P

N

T

F

F

Q

E

I

L

P

S

D

A

G

D

Q

E

M

V

E

E

L

L

G

G

I

L

G

G

H

I

H
|
H

G

G

E

E

P

A

G

G

I

V

D

R

V

R

I

I

P

K

I

M

E

A

P

T

A

A

A

D

A

E

M

I

A

V

E

K

R

L

M

M

L

L

A

D

P

H

I

M

L

T

A

N

D

T

R

T

D

N

F

A

S

S

Q

H

-

V

-

P

-

V

-

Q

-

P

D

G

N

S

S

S

V

V

V

M

L

M

V

V

S

N

G

N

L

L

G

G

A

G

T

L

P

S

V

F

M

L

E

E

L

L

Y

G

I

I

F

I

Y

A

A

D

E

A

V

T

E

V

K

R

Q

S

L

L

T

E

A

G

K

R

G

G

L

V

K

K

I

I

H

A

R

R

C

A

Y

L

V

V

G

G

N

T

Y

F

F

M

T

S

S

A

L

L

E

E

M

M

M

P

G

G

V

I

T

S

L

L

T

T

L

L

G

L

L

V

D

D

A

E

E

P

L

L

T

L

T

K

L

L

I

I

D

D

T112 (≠ A108) mutation to A: Highly decreases kinase activity. No effect on FMN cyclase activity.
A185 (≠ V182) to T: in dbSNP:rs2260655
K204 (= K202) mutation to A: Slightly decreases kinase activity. No effect on FMN cyclase activity.
H221 (= H219) mutation to A: Abolishes kinase activity but not FMN cyclase activity.

Sites not aligning to the query:

401 D→A: Abolishes both kinase and FMN cyclase activities.
403 D→A: Abolishes both kinase and FMN cyclase activities.
404 C→A: Decreases both kinase and FMN cyclase activities.
445 G → S: in TKFCD; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs1590578831
446 S→A: Decreases both kinase and FMN cyclase activities.
543 R → I: in TKFCD; reduced protein levels in patient cells; very severe decrease of triokinase and glycerone kinase activities; dbSNP:rs547013163
556 D→A: Abolishes both kinase and FMN cyclase activities.

P45510 Dihydroxyacetone kinase; DHA kinase; Glycerone kinase; EC 2.7.1.29 from Citrobacter freundii (see 2 papers)
30% identity, 94% coverage: 5:316/333 of query aligns to 6:314/552 of P45510

query
sites
P45510

I

F

N

N

D

Q

P

R

D

T

Q

H

V

L

V

V

E

S

D

D

M

V

L

I

R

D

G

G

I

A

L

I

V

I

A

A

H

S

P

P

-

W

-

N

E

N

L

L

R

A

Q

R

Y

L

E

E

T

S

N

D

P

P

-

A

-

I

R

R

V

I

I

V

V

V

K

R

A

-

K

R

P

D

S

L

G

N

P

K

G

N

R

N

V

V

G

A

I

V

V

I

T

S

G

G

S

S

G

G

H

H

E

E

P

P

A

A

F

H

L

V

G

G

Y

F

V

I

G

G

P

K

G

G

L

M

V

L

D

T

A

A

V

A

A

V

V

C

G

G

E

D

I

V

F

F

S

A

S

S

P

P

T

S

A

V

K

D

S

A

F

V

F

L

D

T

A

A

F

I

R

Q

A

A

A

V

D

T

H

G

G

E

A

A

G

G

V

C

A

L

C

L

L

I

Y

V

G

K

N

N

Y

Y

A

T

G

G

D

D

N

R

M

L

N

N

V

F

K

G

L

L

A

A

M

A

K

E

M

K

A

A

A

R

S

R

K

L

D

G

M

Y

R

N

I

V

R

E

T

M

V

L

V

I

A

V

N

G

D

D

V

I

A

-

S

S

A

L

P

P

K

D

A

N

E

K

I

-

A

-

K

H

R

P

R

R

G

G

V

I

A

A

G

G

E

T

I

I

F

L

M

V

W

H

K

K

I

I

G

A

G

G

A

Y

A

F

A

A

A

E

Q

R

H

G

Y

Y

D

N

L

L

D

A

G

T

V

V

I

L

R

R

V

E

A

A

Q

Q

K

Y

T

A

V

A

D

S

H

N

C

T

R

F

S

S

I

L

G

G

I

V

G

A

L

L

T

S

P

S

C

C

T

H

I

L

A

P

A

Q

V

E

G

T

K

D

P

A

N

A

F

P

Q

R

I

H

P

H

D

P

G

G

Q

H

M

A

E

E

L

L

G

G

I

M

G

G

H

I

H
|
H

G

G

E

E

P

P

G

G

I

A

D

S

V

V

I

I

P

D

I

T

E

Q

P

N

A

S

A

A

A

Q

M

V

A

V

E

N

R

L

M

M

L

V

A

D

P

K

I

L

L

L

A

A

D

-

R

-

D

A

F

L

S

P

Q

E

D

T

N

G

S

R

V

L

V

A

V

V

L

M

V

I

S

N

G

N

L

L

G

G

A

G

T

V

P

S

V

V

M

A

E

E

L

M

Y

A

I

I

F

-

Y

-

A

-

E

-

V

I

E

T

K

R

Q

E

L

L

T

A

A

S

K

S

G

P

L

L

K

H

I

S

H

R

R

I

C

D

Y

W

V

L

-

I

-

G

-

P

G

A

N

S

Y

L

F

V

T

T

S

A

L

L

E

D

M

M

M

K

G

G

V

F

T

S

L

L

T

T

L

A

L

I

G

V

L

L

D

E

A

E

E

S

L

I

H220 (= H219) active site, Tele-hemiaminal-histidine intermediate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
380 D→A: Loss of kinase activity.
385 D→A: Loss of kinase activity.
385:388 binding
387 D→A: Loss of kinase activity.
388 T→H: Reduced kinase activity.
431:432 binding
468 binding
476:477 binding
533:535 binding

1un9A Crystal structure of the dihydroxyacetone kinase from c. Freundii in complex with amp-pnp and mg2+ (see paper)
30% identity, 94% coverage: 5:316/333 of query aligns to 6:314/537 of 1un9A

query
sites
1un9A

I

F

N

N

D

Q

P

R

D

T

Q

H

V

L

V

V

E

S

D

D

M

V

L

I

R

D

G

G

I

A

L

I

V

I

A

A

H

S

P

P

-

W

-

N

E

N

L

L

R

A

Q

R

Y

L

E

E

T

S

N

D

P

P

-

A

-

I

R

R

V

I

I

V

V

V

K

R

A

-

K

R

P

D

S

L

G

N

P

K

G

N

R

N

V

V

G

A

I

V

V

I

T

S

G

G

G
|
G

G

G

S

S

G

G

H
|
H

E

E

P

P

A

A

F

H

L

V

G

G

Y

F

V

I

G

G

P

K

G

G

L

M

V

L

D

T

A

A

V

A

A

V

V

C

G

G

E

D

I

V

F
|
F

S

A

S

S

P

P

T

S

A

V

K

D

S

A

F

V

F

L

D

T

A

A

F

I

R

Q

A

A

A

V

D

T

H

G

G

E

A

A

G

G

V

C

A

L

C

L

L

I

Y

V

G
x
K

N

N

Y

Y

A

T

G

G

D
|
D

N

R

M

L

N

N

V

F

K

G

L

L

A

A

M

A

K

E

M

K

A

A

A

R

S

R

K

L

D

G

M

Y

R

N

I

V

R

E

T

M

V

L

V

I

A

V

N

G

D

D

V

I

A

-

S

S

A

L

P

P

K

D

A

N

E

K

I

-

A

-

K

H

R

P

R

R

G

G

V

I

A

A

G

G

E

T

I

I

F

L

M

V

W

H

K

K

I

I

G

A

G

G

A

Y

A

F

A

A

A

E

Q

R

H

G

Y

Y

D

N

L

L

D

A

G

T

V

V

I

L

R

R

V

E

A

A

Q

Q

K

Y

T

A

V

A

D

S

H

N

C

T

R

F

S

S

I

L

G

G

I

V

G

A

L

L

T

S

P

S

C

C

T

H

I

L

A

P

A

Q

V

E

G

T

K

D

P

A

N

A

F

P

Q

R

I

H

P

H

D

P

G

G

Q

H

M

A

E

E

L

L

G

G

I

M

G

G

H

I

H
|
H

G

G

E

E

P

P

G

G

I

A

D

S

V

V

I

I

P

D

I

T

E

Q

P

N

A

S

A

A

A

Q

M

V

A

V

E

N

R

L

M

M

L

V

A

D

P

K

I

L

L

L

A

A

D

-

R

-

D

A

F

L

S

P

Q

E

D

T

N

G

S

R

V

L

V

A

V

V

L

M

V

I

S

N

G

N

L

L

G

G

A

G

T

V

P

S

V

V

M

A

E

E

L

M

Y

A

I

I

F

-

Y

-

A

-

E

-

V

I

E

T

K

R

Q

E

L

L

T

A

A

S

K

S

G

P

L

L

K

H

I

S

H

R

R

I

C

D

Y

W

V

L

-

I

-

G

-

P

G

A

N

S

Y

L

F

V

T

T

S

A

L

L

E

D

M

M

M

K

G

G

V

F

T

S

L

L

T

T

L

A

L

I

G

V

L

L

D

E

A

E

E

S

L

I

binding Dihydroxyacetone: G57 (= G53), H61 (= H57), F83 (= F79), K109 (≠ G105), D114 (= D110), H220 (= H219)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 385, 387, 388, 391, 430, 431, 432, 435, 468, 470, 476, 477, 520, 522
binding magnesium ion: 380, 385, 385, 387, 387

Query Sequence

>GFF2361 FitnessBrowser__WCS417:GFF2361
MNRVINDPDQVVEDMLRGILVAHPELRQYETNPRVIVKAKPSGPGRVGIVTGGGSGHEPA
FLGYVGPGLVDAVAVGEIFSSPTAKSFFDAFRAADHGAGVACLYGNYAGDNMNVKLAMKM
AASKDMRIRTVVANDDVASAPKAEIAKRRGVAGEIFMWKIGGAAAAQHYDLDGVIRVAQK
TVDHCRSIGIGLTPCTIAAVGKPNFQIPDGQMELGIGHHGEPGIDVIPIEPAAAMAERML
APILADRDFSQDNSVVVLVSGLGATPVMELYIFYAEVEKQLTAKGLKIHRCYVGNYFTSL
EMMGVTLTLLGLDAELTTLIDQPCRSIGMTQAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory