SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2362 FitnessBrowser__WCS417:GFF2362 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
35% identity, 91% coverage: 32:369/371 of query aligns to 18:345/348 of O58389

query
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O58389

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C42 (= C56) binding Zn(2+)
T44 (≠ G58) mutation to A: Total loss of enzymatic activity.
H67 (= H87) binding Zn(2+)
E68 (= E88) binding Zn(2+)
C97 (= C119) binding Zn(2+)
C100 (= C122) binding Zn(2+)
C103 (= C125) binding Zn(2+)
C111 (= C133) binding Zn(2+)
E152 (= E177) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (= L205) binding NAD(+)
E199 (≠ D225) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
R204 (= R230) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ FSV 292:294) binding NAD(+)
IT 291:292 (vs. gap) binding NAD(+)
R294 (≠ S317) mutation to A: 4000-fold decrease in catalytic efficiency.

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
35% identity, 91% coverage: 32:369/371 of query aligns to 16:343/346 of 2dfvA

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2dfvA

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active site: C40 (= C56), G41 (≠ M57), T42 (≠ G58), H45 (≠ K61), H65 (= H87), E66 (= E88), C95 (= C119), C98 (= C122), C101 (= C125), C109 (= C133), K113 (≠ D137), P151 (= P178), A155 (≠ S182), K340 (= K366)
binding nicotinamide-adenine-dinucleotide: G175 (= G203), P176 (≠ T204), L177 (= L205), E197 (≠ D225), P198 (≠ M226), R202 (= R230), F241 (≠ A269), S242 (≠ T270), A244 (≠ H272), L264 (≠ F292), G265 (≠ S293), L266 (≠ V294), I289 (vs. gap), T290 (vs. gap)
binding zinc ion: C95 (= C119), C101 (= C125), C109 (= C133)

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
34% identity, 95% coverage: 20:371/371 of query aligns to 5:347/350 of Q5JI69

query
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Q5JI69

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L179 (= L205) binding NAD(+)
E199 (≠ D225) binding NAD(+)
R204 (= R230) binding NAD(+)
LGL 266:268 (≠ FSV 292:294) binding NAD(+)
IT 291:292 (≠ -L 315) binding NAD(+)

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
34% identity, 95% coverage: 20:371/371 of query aligns to 3:345/347 of 3gfbA

query
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3gfbA

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N

-

N

D

V

M

Q

D

G

G

A

V

M

F

A

A

Q

H

Y

Y

M

A

I

I

F

V

T

P

K

A

E

K

G

N

I

A

I

-

H

W

K

K

V

N

P

P

D

K

S

D

I

M

A

P

P

P

D

E

E

Y

A

A

I

A

L

L

I

Q

E

E

P
|
P

L

L

A

G

C

N

S
x
A

L

V

H

D

A

T

A

V

E

L

R

A

A

G

N

P

V

I

D

A

F

-

D

G

D

R

V

S

V

T

V

L

V

I

A

T

G
|
G

A

A

G
|
G

T
x
P

L
|
L

G

G

L

L

G

L

I

G

I

I

G

A

A

V

V

A

R

K

M

A

R

S

N

G

P

A

K

Y

K

P

L

V

I

I

V

V

L
x
S

D
x
E

M
x
P

K

S

P

E

E

F

R
|
R

A

R

A

K

L

L

A

A

L

K

R

K

M

V

G

G

A

A

D

D

E

Y

V

V

W

V

N

N

P

P

A

F

E

E

D

D

V

P

L

V

A

K

K

F

I

V

R

M

E

D

I

I

T

T

D

D

G

G

Y

A

G

G

C

V

D

E

I

V

Y

F

I

L

E

E

A
x
F

T
x
S

G

G

H
x
A

H

P

K

K

A

A

V

L

N

E

Q

Q

G

G

L

L

A

K

M

A

L

V

R

T

K

P

L

G

G

G

R

R

F

V

V

S

E

L

F
x
L

S
x
G

V
x
L

F

F

N

P

D

R

E

E

A

V

T

T

V

I

D

D

W

F

S

N

I

N

I

L

G

I

D

I

R

F

K

K

E

A

L

L

D

E

V

V

-

H

-

G

-
x
I

L
x
T

G

G

S

R

H

H

L

L

G

W

P

E

Y

T

M

W

Y

Y

P

T

R

V

A

S

I

-

D

S

F

L

I

I

G

Q

N

S

R

G

K

K

I

L

D

N

M

L

R

D

D

P

V

I

T

T

H

H

T

K

F

Y

A

K

L

G

A

F

D

D

-

K

F

F

K

E

E

E

A

A

F

F

A

E

V

L

M

M

E

-

R

R

G

A

D

G

K

K

S

T

L

G

K
|
K

V

V

L

F

Q

F

P

P

active site: C40 (= C56), G41 (≠ M57), T42 (≠ G58), H45 (≠ K61), H65 (= H87), E66 (= E88), C95 (= C119), C98 (= C122), C101 (= C125), C109 (= C133), K113 (≠ D137), P151 (= P178), A155 (≠ S182), K340 (= K366)
binding nicotinamide-adenine-dinucleotide: G173 (= G201), G175 (= G203), P176 (≠ T204), L177 (= L205), S196 (≠ L224), E197 (≠ D225), P198 (≠ M226), R202 (= R230), F241 (≠ A269), S242 (≠ T270), A244 (≠ H272), L264 (≠ F292), G265 (≠ S293), L266 (≠ V294), I289 (vs. gap), T290 (≠ L315)

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
31% identity, 88% coverage: 28:355/371 of query aligns to 11:328/347 of 5vm2A

query
sites
5vm2A

P

P

E

G

D

T

Y

M

R

K

L

I

E

I

T

S

V

A

D

E

V

I

P

P

V

V

P

P

G

K

P

E

D

D

E

E

I

V

L

L

T

I

K

K

V

V

E

E

L

Y

C

V

G

G

I

I

C
|
C

M
x
G

G
x
S

D

D

I

V

K
x
H

T

G

Y

F

R

E

G

S

A

G

P

P

S

F

F

I

W

-

G

-

D

-

A

-

E

-

Q

-

P

-

R

-

Y

-

V

-

K

-

P

P

M

K

I

D

P

P

-

N

-

Q

-

E

-

I

-

G

-

L

G

G

H
|
H

E
|
E

F

C

V

A

C

G

R

T

V

V

A

A

L

V

G

G

P

-

G

-

A

S

E

R

K

V

R

R

G

K

V

F

K

K

V

P

G

G

D

D

R

R

V

V

I

N

S

I

E

E

Q

P

I

G

V

V

P

P

C
|
C

W

G

G

H

C
|
C

R

R

F

Y

C
|
C

N

L

H

E

G

G

Q

K

Y

Y

W

N

M

I

C
|
C

Q

P

K

D

H

V

D
|
D

L

F

Y

M

G

A

F

T

Q

Q

N

P

N

N

V

Y

Q

R

G

G

A

A

M

L

A

T

Q

H

Y

Y

M

L

I

C

F

H

T

-

K

P

E

E

G

S

I

F

I

T

H

Y

K

K

V

L

P

P

D

D

S

N

I

M

A

D

P

T

D

M

E

E

A

G

I

T

L

L

I

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E
|
E

P
|
P

L

A

A

A

C

V

S
x
G

L

M

H

H

A

A

E

M

R

L

A

A

N

D

V

V

D

K

F

P

D

G

D

K

V

K

V

I

A

L

G

G

A

A

G

G

T

C

L

I

G

G

L

L

G

M

I

T

I

L

G

Q

A

A

V

C

R

K

M

C

R

L

N

G

P

A

K

T

K

E

L

I

I

A

V

V

L

V

D

D

M

V

K

L

P

E

E

K

R

R

A

L

A

A

L

M

A

A

L

E

R

Q

M

L

G

G

A

A

D

T

E

V

V

V

W

I

N

N

P

G

A

A

E

K

E

E

D

D

V

T

L

I

A

A

K

R

I

C

R

Q

E

Q

I

F

T

T

D

E

G

D

Y

M

G

G

C

A

D

D

I

I

Y

V

I

F

E

E

A

T

T

A

G

G

H

S

H

A

K

V

A

T

V

V

N

K

Q

Q

G

A

L

P

A

Y

M

L

L

V

R

M

K

R

L

G

G

G

R

K

F

I

V

M

E

I

F

V

S

G

V

T

F

V

N

P

D

G

E

A

A

S

T

A

V

I

D

N

W

F

S

L

I

K

I

I

G

-

D

-

R

N

K

R

E

E

L

V

D

T

V

I

L

Q

G

T

S

V

H

F

L

R

G

Y

P

A

Y

N

M

R

Y

Y

P

P

R

V

A

T

I

I

D

E

F

A

I

I

G

S

N

S

R

G

K

R

I

F

D

D

M

V

R

K

D

S

V

M

V

V

T

T

H

H

T

I

F

Y

A

D

L

Y

A

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D

D

F

V

K

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E

Q

A

A

F

F

active site: C39 (= C56), G40 (≠ M57), S41 (≠ G58), H44 (≠ K61), H65 (= H87), E66 (= E88), C95 (= C119), C98 (= C122), C101 (= C125), C109 (= C133), D113 (= D137), P153 (= P178), G157 (≠ S182)
binding magnesium ion: H65 (= H87), E66 (= E88), E152 (= E177)
binding zinc ion: C95 (= C119), C98 (= C122), C101 (= C125), C109 (= C133)

Sites not aligning to the query:

active site: 340

2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
30% identity, 95% coverage: 20:371/371 of query aligns to 1:341/343 of 2ejvA

query
sites
2ejvA

M

M

Q

R

A

A

V

L

V

A

C

K

H

L

G

A

P

P

E

E

D

E

Y

-

R

G

L

L

E

T

T

L

V

V

D

D

V

R

P

P

V

V

P

P

-

E

-

P

G

G

P

P

D

G

E

E

I

I

L

L

T

V

K

R

V

V

E

E

L

A

C

A

G

S

I

I

C
|
C

M
x
G

G
x
T

D

D

I

L

K
x
H

T

I

Y

W

R

K

G

-

A

-

P

-

S

-

F

-

W

W

G

-

D

D

A

A

E

W

Q

A

P

R

R

G

Y

R

V

I

K

R

P

P

M

L

I

V

P

T

G

G

H
|
H

E
|
E

F

F

V

S

C

G

R

V

V

V

V

E

A

A

L

V

G

G

P

P

G

G

A

V

E

R

K

R

R

P

G

-

V

-

K

Q

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V

G

G

D

D

R

H

V

V

I

S

S

L

E

E

Q

S

I

H

V

I

P

V

C
|
C

W

H

G

A

C
|
C

R

P

F

A

C
|
C

N

R

H

T

G

G

Q

N

Y

Y

W

H

M

V

C
|
C

Q

L

K

N

H

T

D
x
Q

L

I

Y

L

G

G

F

V

Q

D

N

R

N

D

V

-

Q

-

G

G

A

G

M

F

A

A

Q

E

Y

Y

M

V

I

V

F

V

T

P

K

A

E

E

G

N

I

A

I

W

H

V

K

N

V

-

P

P

D

K

S

D

I

L

A

P

P

F

D

E

E

V

A

A

I

A

L

I

I

L

E

E

P
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P

L

F

A

G

C

N

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x
A

L

V

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H

A

T

A

V

E

Y

R

A

A

G

N

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G

D

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F

S

D

G

D

K

V

S

V

V

V

L

V

I

A

T

G
|
G

A

A

G
|
G

T
x
P

L
x
I

G

G

L

L

G

M

I

A

G

M

A

V

V

V

R

R

M

A

R

S

N

G

P

A

K

G

K

P

L

I

I

L

V

V

L
x
S

D
|
D

M
x
P

K

N

P

P

E

Y

R
|
R

A

L

A

A

L

F

A

A

L

-

R

R

M

P

G

Y

A

A

D

D

E

R

V

L

W

V

N

N

P

P

A

L

E

E

D

D

V

L

L

L

A

E

K

V

I

V

R

R

E

R

I

V

T

T

D

-

G

G

Y

S

G

G

C

V

D

E

I

V

Y

L

I

L

E

E

A
x
F

T
x
S

G

G

H
x
N

H

E

K

A

A
|
A

V

I

N

H

Q

Q

G

G

L

L

A

M

M

A

L

L

R

I

K

P

L

G

G

G

R

E

F

A

V

R

E

I

F
x
L

S
x
G

V
x
I

F

P

N

S

D

D

E

P

A

I

T

R

V

F

D

D

W

L

S

A

-

G

-

E

-

L

I

V

I

M

G

R

D

G

R

I

K

T

E

A

L

F

D

G

V

I

L

A

G

G

S

R

H

R

L

L

-

W

G

Q

P

T

Y

W

M

M

Y

Q

P

G

R

T

A

A

I

L

D

V

F

Y

I

S

G

G

N

-

R

-

K

R

I

V

D

D

M

L

R

S

D

P

V

L

T

T

H

H

T

R

F

L

A

P

L

L

A

S

D

R

F

Y

K

R

E

E

A

A

F

F

A

G

V

L

M

L

E

A

R

S

G

G

D

-

K

Q

S

A

L

V

K
|
K

V

V

V

I

L

L

Q

D

P

P

active site: C38 (= C56), G39 (≠ M57), T40 (≠ G58), H43 (≠ K61), H63 (= H87), E64 (= E88), C93 (= C119), C96 (= C122), C99 (= C125), C107 (= C133), Q111 (≠ D137), P149 (= P178), A153 (≠ S182), K336 (= K366)
binding nicotinamide-adenine-dinucleotide: G172 (= G201), G174 (= G203), P175 (≠ T204), I176 (≠ L205), S195 (≠ L224), D196 (= D225), P197 (≠ M226), R201 (= R230), F238 (≠ A269), S239 (≠ T270), N241 (≠ H272), A244 (= A275), L261 (≠ F292), G262 (≠ S293), I263 (≠ V294)
binding zinc ion: C38 (= C56), H63 (= H87), E64 (= E88), C96 (= C122), C99 (= C125), C107 (= C133)

2dq4A Crystal structure of threonine 3-dehydrogenase
30% identity, 95% coverage: 20:371/371 of query aligns to 1:341/343 of 2dq4A

query
sites
2dq4A

M

M

Q

R

A

A

V

L

V

A

C

K

H

L

G

A

P

P

E

E

D

E

Y

-

R

G

L

L

E

T

T

L

V

V

D

D

V

R

P

P

V

V

P

P

-

E

-

P

G

G

P

P

D

G

E

E

I

I

L

L

T

V

K

R

V

V

E

E

L

A

C

A

G

S

I

I

C
|
C

M
x
G

G
x
T

D

D

I

L

K
x
H

T

I

Y

W

R

K

G

-

A

-

P

-

S

-

F

-

W

W

G

-

D

D

A

A

E

W

Q

A

P

R

R

G

Y

R

V

I

K

R

P

P

M

L

I

V

P

T

G

G

H
|
H

E
|
E

F

F

V

S

C

G

R

V

V

V

V

E

A

A

L

V

G

G

P

P

G

G

A

V

E

R

K

R

R

P

G

-

V

-

K

Q

V

V

G

G

D

D

R

H

V

V

I

S

S

L

E

E

Q

S

I

H

V

I

P

V

C
|
C

W

H

G

A

C
|
C

R

P

F

A

C
|
C

N

R

H

T

G

G

Q

N

Y

Y

W

H

M

V

C
|
C

Q

L

K

N

H

T

D
x
Q

L

I

Y

L

G

G

F

V

Q

D

N

R

N

D

V

-

Q

-

G

G

A

G

M

F

A

A

Q

E

Y

Y

M

V

I

V

F

V

T

P

K

A

E

E

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N

I

A

I

W

H

V

K

N

V

-

P

P

D

K

S

D

I

L

A

P

P

F

D

E

E

V

A

A

I

A

L

I

I

L

E

E

P
|
P

L

F

A

G

C

N

S
x
A

L

V

H

H

A

T

A

V

E

Y

R

A

A

G

N

S

V

G

D

V

F

S

D

G

D

K

V

S

V

V

V

L

V

I

A

T

G

G

A

A

G

G

T

P

L

I

G

G

L

L

G

M

I

A

G

M

A

V

V

V

R

R

M

A

R

S

N

G

P

A

K

G

K

P

L

I

I

L

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V

L

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D

D

M

P

K

N

P

P

E

Y

R

R

A

L

A

A

L

F

A

A

L

-

R

R

M

P

G

Y

A

A

D

D

E

R

V

L

W

V

N

N

P

P

A

L

E

E

D

D

V

L

L

L

A

E

K

V

I

V

R

R

E

R

I

V

T

T

D

-

G

G

Y

S

G

G

C

V

D

E

I

V

Y

L

I

L

E

E

A

F

T

S

G

G

H

N

H

E

K

A

A

A

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I

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Q

Q

G

G

L

L

A

M

M

A

L

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L

G

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A

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R

E

I

F

L

S

G

V

I

F

P

N

S

D

D

E

P

A

I

T

R

V

F

D

D

W

L

S

A

-

G

-

E

-

L

I

V

I

M

G

R

D

G

R

I

K

T

E

A

L

F

D

G

V

I

L

A

G

G

S

R

H

R

L

L

-

W

G

Q

P

T

Y

W

M

M

Y

Q

P

G

R

T

A

A

I

L

D

V

F

Y

I

S

G

G

N

-

R

-

K

R

I

V

D

D

M

L

R

S

D

P

V

L

T

T

H

H

T

R

F

L

A

P

L

L

A

S

D

R

F

Y

K

R

E

E

A

A

F

F

A

G

V

L

M

L

E

A

R

S

G

G

D

-

K

Q

S

A

L

V

K
|
K

V

V

V

I

L

L

Q

D

P

P

active site: C38 (= C56), G39 (≠ M57), T40 (≠ G58), H43 (≠ K61), H63 (= H87), E64 (= E88), C93 (= C119), C96 (= C122), C99 (= C125), C107 (= C133), Q111 (≠ D137), P149 (= P178), A153 (≠ S182), K336 (= K366)
binding zinc ion: C38 (= C56), H63 (= H87), E64 (= E88), C93 (= C119), C96 (= C122), C107 (= C133)

Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
30% identity, 95% coverage: 20:371/371 of query aligns to 1:341/343 of Q5SKS4

query
sites
Q5SKS4

M

M

Q

R

A

A

V

L

V

A

C

K

H

L

G

A

P

P

E

E

D

E

Y

-

R

G

L

L

E

T

T

L

V

V

D

D

V

R

P

P

V

V

P

P

-

E

-

P

G

G

P

P

D

G

E

E

I

I

L

L

T

V

K

R

V

V

E

E

L

A

C

A

G

S

I

I

C
|
C

M

G

G

T

D

D

I

L

K

H

T

I

Y

W

R

K

G

-

A

-

P

-

S

-

F

-

W

W

G

-

D

D

A

A

E

W

Q

A

P

R

R

G

Y

R

V

I

K

R

P

P

M

L

I

V

P

T

G

G

H
|
H

E
|
E

F

F

V

S

C

G

R

V

V

V

V

E

A

A

L

V

G

G

P

P

G

G

A

V

E

R

K

R

R

P

G

-

V

-

K

Q

V

V

G

G

D

D

R

H

V

V

I

S

S

L

E

E

Q

S

I

H

V

I

P

V

C
|
C

W

H

G

A

C
|
C

R

P

F

A

C
|
C

N

R

H

T

G

G

Q

N

Y

Y

W

H

M

V

C
|
C

Q

L

K

N

H

T

D

Q

L

I

Y

L

G

G

F

V

Q

D

N

R

N

D

V

-

Q

-

G

G

A

G

M

F

A

A

Q

E

Y

Y

M

V

I

V

F

V

T

P

K

A

E

E

G

N

I

A

I

W

H

V

K

N

V

-

P

P

D

K

S

D

I

L

A

P

P

F

D

E

E

V

A

A

I

A

L

I

I

L

E

E

P

P

L

F

A

G

C

N

S

A

L

V

H

H

A

T

A

V

E

Y

R

A

A

G

N

S

V

G

D

V

F

S

D

G

D

K

V

S

V

V

V

L

V

I

A

T

G

G

A

A

G

G

T

P

L
x
I

G

G

L

L

G

M

I

A

G

M

A

V

V

V

R

R

M

A

R

S

N

G

P

A

K

G

K

P

L

I

I

L

V

V

L

S

D
|
D

M

P

K

N

P

P

E

Y

R
|
R

A

L

A

A

L

F

A

A

L

-

R

R

M

P

G

Y

A

A

D

D

E

R

V

L

W

V

N

N

P

P

A

L

E

E

D

D

V

L

L

L

A

E

K

V

I

V

R

R

E

R

I

V

T

T

D

-

G

G

Y

S

G

G

C

V

D

E

I

V

Y

L

I

L

E

E

A

F

T

S

G

G

H

N

H

E

K

A

A

A

V

I

N

H

Q

Q

G

G

L

L

A

M

M

A

L

L

R

I

K

P

L

G

G

G

R

E

F

A

V

R

E

I

F
x
L

S
x
G

V
x
I

F

P

N

S

D

D

E

P

A

I

T

R

V

F

D

D

W

L

S

A

-

G

-

E

-

L

I

V

I

M

G

R

D

G

R

I

K

T

E

A

L

F

D

G

V
x
I

L
x
A

G
|
G

S

R

H

R

L

L

-

W

G

Q

P

T

Y

W

M

M

Y

Q

P

G

R

T

A

A

I

L

D

V

F

Y

I

S

G

G

N

-

R

-

K

R

I

V

D

D

M

L

R

S

D

P

V

L

T

T

H

H

T

R

F

L

A

P

L

L

A

S

D

R

F

Y

K

R

E

E

A

A

F

F

A

G

V

L

M

L

E

A

R

S

G

G

D

-

K

Q

S

A

L

V

K

K

V

V

V

I

L

L

Q

D

P

P

C38 (= C56) binding Zn(2+)
H63 (= H87) binding Zn(2+)
E64 (= E88) binding Zn(2+)
C93 (= C119) binding Zn(2+)
C96 (= C122) binding Zn(2+)
C99 (= C125) binding Zn(2+)
C107 (= C133) binding Zn(2+)
I176 (≠ L205) binding NAD(+)
D196 (= D225) binding NAD(+)
R201 (= R230) binding NAD(+)
LGI 261:263 (≠ FSV 292:294) binding NAD(+)
IAG 286:288 (≠ VLG 314:316) binding NAD(+)

P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
29% identity, 94% coverage: 22:370/371 of query aligns to 9:346/354 of P07846

query
sites
P07846

A

S

V

L

V

V

C

V

H

H

G

G

P

P

E

G

D

D

Y

L

R

R

L

L

E

E

T

N

V

Y

D

P

V

I

P

P

V

E

P

P

G

G

P

P

D

N

E

E

I

V

L

L

T

L

K

K

V

M

E

H

L

S

C

V

G

G

I

I

C
|
C

M

G

G

S

D

D

I

V

K

H

T
x
Y

Y

W

R

Q

G

G

A

R

P

-

S

-

F

-

W

I

G

G

D

D

A

F

E

-

Q

-

P

-

R

-

Y

V

V

V

K

K

P

K

P

P

M

M

I

V

P

L

G

G

H
|
H

E
|
E

F

A

V

S

C

G

R

T

V

V

A

K

L

V

G

G

P

-

G

-

A

S

E

L

K

V

R

R

G

H

V

L

K

Q

V

P

G

G

D

D

R

R

V

V

I

A

S

I

E

Q

Q

P

I

G

V

A

P

P

C

R

W

Q

G

T

C

D

R

E

F

F

C

C

N

K

H

I

G

G

Q

R

Y

Y

W

N

M

L

C

-

Q

S

K

P

H

T

D

I

L

F

Y

F

G

C

F

A

Q

T

N

P

V

D

Q

D

G

G

A

N

M

L

A

C

Q

R

Y

F

M

Y

I

K

F

H

T

N

K

A

E

N

G

-

I

F

I

C

H

Y

K

K

V

L

P

P

D

D

S

N

I

V

A

T

P

F

D

E

E

E

A

G

I

A

L

L

I

I

E
|
E

P

P

L

L

A

S

C

V

S

G

L

I

H

H

A

A

A

C

E

R

R

R

A

A

N

G

V

V

D

T

F

L

D

G

D

N

V

K

V

V

V

L

V

V

A

C

G

G

A

A

G

G

T

P

L

I

G

G

L

L

G

V

I

N

I

L

G

L

A

A

V

A

R

K

M

A

R

M

N

G

P

A

K

A

K

Q

L

V

I

V

V

V

L

T

D

D

M

L

K

S

P

A

E

S

R

R

A

L

A

S

L

K

A

A

L

K

R

E

M

V

G

G

A

A

D

D

-

F

-

I

-

L

E

E

V

I

W

S

N

N

P

E

A

S

E

P

E

E

D

E

V

I

L

A

A

K

K

K

I

V

R

E

E

G

I

L

T

L

D

-

G

G

Y

S

G

K

C

P

D

E

I

V

Y

T

I

I

E

E

A

C

T

T

G

G

H

V

H

E

K

T

A

S

V

I

N

Q

Q

A

G

G

L

I

A

Y

M

A

L

T

R

H

K

S

L

G

G

G

R

T

F

L

V

V

E

L

F

V

S

G

V

L

F

G

N

S

D

E

E

M

A

T

T

S

V

V

D

P

W

L

S

-

I

V

I

H

G

A

D

A

R

T

K

R

E

E

L

V

D

D

V

I

L

K

G

G

S

V

H

F

L
x
R

G
x
Y

P

C

Y

N

M

T

Y

W

P

P

R

M

A

A

I

I

D

S

F

M

I

L

G

A

N

S

R

K

K

S

I

V

D

N

M

V

R

K

D

P

V

L

V

V

T

T

H

H

T

R

F

F

A

P

L

L

A

E

D

K

F

A

K

L

E

E

A

A

F

F

A

E

V

T

M

S

E

K

R

K

G

G

D

-

K

L

S

G

L

L

K

K

V

V

V

M

L

I

Q

K

C43 (= C56) binding Zn(2+)
Y49 (≠ T62) binding substrate
H67 (= H87) binding Zn(2+)
E68 (= E88) binding Zn(2+)
E153 (= E177) binding substrate
R296 (≠ L319) binding substrate
Y297 (≠ G320) binding substrate

Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
28% identity, 94% coverage: 22:370/371 of query aligns to 11:349/357 of Q00796

query
sites
Q00796

A

S

V

L

V

V

C

V

H

H

G

G

P

P

E

G

D

D

Y

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R

R

L

L

E

E

T

N

V

Y

D

P

V

I

P

P

V

E

P

P

G

G

P

P

D

N

E

E

I

V

L

L

T

L

K

R

V

M

E

H

L

S

C

V

G

G

I

I

C
|
C

M

G

G

S

D

D

I

V

K

H

T

-

Y

-

R

-

G

-

A

-

P

-

S

-

F

Y

W

W

G

E

D

Y

A

G

E

R

Q

I

P

G

R

N

Y

F

-

I

V

V

K

K

P

K

P

P

M

M

I

V

P

L

G

G

H
|
H

E
|
E

F

A

V

S

C

G

R

T

V

V

V

E

A

K

L

V

G

G

P

-

G

-

A

S

E

S

K

V

R

K

G

H

V

L

K

K

V

P

G

G

D

D

R

R

V

V

I

A

S

I

E

E

Q

P

I

G

V

A

P

P

C

R

W

E

G

N

C

D

R

E

F

F

C

C

N

K

H

M

G

G

Q
x
R

Y

Y

W

N

M

L

C

-

Q

S

K

P

H

S

D

I

L

F

Y

F

G

C

F

A

Q

T

N

P

V

D

Q

D

G

G

A

N

M

L

A

C

Q

R

Y

F

M

Y

I

K

F
x
H

T

N

K

A

E

-

G

A

I

F

I

C

H

Y

K

K

V

L

P

P

D

D

S

N

I

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A

T

P

F

D

E

E

E

A

G

I
x
A

L

L

I

I

E

E

P

P

L

L

A

S

C

V

S

G

L

I

H

H

A

A

A

C

E

R

R

R

A

G

N

G

V

V

D

T

F

L

D

G

D

H

V

K

V

V

V

L

V

V

A

C

G

G

A

A

G

G

T

P

L
x
I

G

G

L

M

G

V

I

T

I

L

G

L

A

V

V

A

R

K

M

A

R

M

N

G

P

A

K

A

K

Q

L

V

I

V

V

V

L

T

D
|
D

M

L

K

S

P

A

E

T

R
|
R

A

L

A

S

L

K

A

A

L

K

R

E

M

I

G

G

A

A

D

D

E

L

V

V

W

L

N

Q

P

I

A

S

E

K

E

E

D

S

V

P

L

Q

A

E

K

I

I

A

R

R

E

K

I

V

T

E

D

G

G
x
Q

Y

L

G

G

C

C

-

K

-

P

D

E

I

V

Y

T

I

I

E

E

A

C

T

T

G

G

H

A

H

E

K

A

A

S

V

I

N

Q

Q

A

G

G

L

I

A

Y

M

A

L

T

R

R

K

S

L

G

G

G

R
x
N

F

L

V

V

E

L

F
x
V

S
x
G

V
x
L

F

G

N

S

D

E

E

M

A

T

T

T

V

V

D

P

W

L

S

-

I

L

I

H

G

A

D

A

R

I

K

R

E

E

L

V

D

D

V

I

L

K

G

G

S
x
V

H
x
F

L
x
R

G

Y

P

C

Y

N

M

T

Y

W

P

P

R

V

A

A

I

I

D

S

F

M

I

L

G

A

N

S

R

K

K

S

I

V

D

N

M

V

R

K

D

P

V

L

V
|
V

T

T

H

H

T

R

F

F

A

P

L

L

A

E

D

K

F

A

K

L

E

E

A

A

F

F

A

E

V

T

M

F

E

K

R

K

G

G

D

-

K

L

S

G

L

L

K

K

V

I

V

M

L

L

Q

K

C45 (= C56) binding Zn(2+)
H70 (= H87) binding Zn(2+)
E71 (= E88) binding Zn(2+)
R110 (≠ Q129) to P: in HMNR8; results in protein aggregation
H135 (≠ F155) to R: in HMNR8; results in protein aggregation
A153 (≠ I174) to D: in HMNR8; uncertain significance; results in protein aggregation; dbSNP:rs145813597
I184 (≠ L205) binding NAD(+)
D204 (= D225) binding NAD(+)
R209 (= R230) binding NAD(+)
Q239 (≠ G260) to L: in dbSNP:rs1042079
N269 (≠ R288) to T: in dbSNP:rs930337
VGL 273:275 (≠ FSV 292:294) binding NAD(+)
VFR 297:299 (≠ SHL 317:319) binding NAD(+)
V322 (= V342) to I: in HMNR8; uncertain significance; dbSNP:rs149975952

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1pl6A Human sdh/nadh/inhibitor complex (see paper)
28% identity, 94% coverage: 22:370/371 of query aligns to 10:348/356 of 1pl6A

query
sites
1pl6A

A

S

V

L

V

V

C

V

H

H

G

G

P

P

E

G

D

D

Y

L

R

R

L

L

E

E

T

N

V

Y

D

P

V

I

P

P

V

E

P

P

G

G

P

P

D

N

E

E

I

V

L

L

T

L

K

R

V

M

E

H

L

S

C

V

G

G

I

I

C
|
C

M
x
G

G
x
S

D

D

I

V

K
x
H

T

-

Y

-

R

-

G

-

A

-

P

-

S

-

F

Y

W

W

G

E

D

Y

A

G

E

R

Q
x
I

P

G

R

N

Y
x
F

-

I

V

V

K

K

P

K

P

P

M

M

I

V

P

L

G

G

H
|
H

E
|
E

F

A

V

S

C

G

R

T

V

V

V

E

A

K

L

V

G

G

P

-

G

-

A

S

E

S

K

V

R

K

G

H

V

L

K

K

V

P

G

G

D

D

R

R

V

V

I

A

S

I

E

E

Q

P

I

G

V

A

P

P

C
x
R

W

E

G

N

C
x
D

R

E

F

F

C
|
C

N

K

H

M

G

G

Q

R

Y

Y

W

N

M

L

C

-

Q
x
S

K

P

H

S

D

I

L
x
F

Y

F

G

C

F

A

Q

T

N

P

V

D

Q

D

G

G

A

N

M

L

A

C

Q

R

Y

F

M

Y

I

K

F

H

T

N

K

A

E

-

G

A

I

F

I

C

H

Y

K

K

V

L

P

P

D

D

S

N

I

V

A

T

P

F

D

E

E

E

A

G

I

A

L

L

I

I

E
|
E

P
|
P

L

L

A

S

C

V

S
x
G

L

I

H

H

A

A

A

C

E

R

R

R

A

G

N

G

V

V

D

T

F

L

D

G

D

H

V

K

V

V

V

L

V

V

A

C

G

G

A

A

G
|
G

T
x
P

L
x
I

G

G

L

M

G

V

I

T

I

L

G

L

A

V

V

A

R

K

M

A

R

M

N

G

P

A

K

A

K

Q

L

V

I

V

V

V

L

T

D
|
D

M
x
L

K

S

P

A

E

T

R
|
R

A

L

A

S

L

K

A

A

L

K

R

E

M

I

G

G

A

A

D

D

E

L

V

V

W

L

N

Q

P

I

A

S

E

K

E

E

D

S

V

P

L

Q

A

E

K

I

I

A

R

R

E

K

I

V

T

E

D

G

G

Q

Y

L

G

G

C

C

-

K

-

P

D

E

I

V

Y

T

I

I

E

E

A
x
C

T
|
T

G

G

H

A

H

E

K

A

A

S

V

I

N

Q

Q

A

G

G

L

I

A

Y

M

A

L

T

R

R

K

S

L

G

G

G

R

T

F

L

V

V

E

L

F
x
V

S
x
G

V
x
L

F

G

N

S

D

E

E

M

A

T

T

T

V

V

D

P

W

L

S

-

I

L

I

H

G

A

D

A

R

I

K

R

E

E

L

V

D

D

V

I

L

K

G

G

S

V

H
x
F

L
x
R

G

Y

P

C

Y

N

M

T

Y

W

P

P

R

V

A

A

I

I

D

S

F

M

I

L

G

A

N

S

R

K

K

S

I

V

D

N

M

V

R

K

D

P

V

L

V

V

T

T

H

H

T

R

F

F

A

P

L

L

A

E

D

K

F

A

K

L

E

E

A

A

F

F

A

E

V

T

M

F

E

K

R

K

G

G

D

-

K

L

S

G

L

L

K
|
K

V

I

V

M

L

L

Q

K

active site: C44 (= C56), G45 (≠ M57), S46 (≠ G58), H49 (≠ K61), H69 (= H87), E70 (= E88), R99 (≠ C119), D102 (≠ C122), C105 (= C125), S113 (≠ Q134), F117 (≠ L138), P156 (= P178), G160 (≠ S182), K344 (= K366)
binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C56), S46 (≠ G58), I56 (≠ Q75), F59 (≠ Y78), H69 (= H87), E155 (= E177), L274 (≠ V294), F297 (≠ H318)
binding nicotinamide-adenine-dinucleotide: G181 (= G203), P182 (≠ T204), I183 (≠ L205), D203 (= D225), L204 (≠ M226), R208 (= R230), C249 (≠ A269), T250 (= T270), V272 (≠ F292), G273 (≠ S293), L274 (≠ V294), F297 (≠ H318), R298 (≠ L319)
binding zinc ion: C44 (= C56), H69 (= H87)

1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
29% identity, 94% coverage: 22:370/371 of query aligns to 4:345/348 of 1e3jA

query
sites
1e3jA

A

S

V

A

V

V

C

L

H

Y

G

K

P

Q

E

N

D

D

Y

L

R

R

L

L

E

E

T

Q

V

R

D

P

V

I

P

P

V

E

P

P

G

K

P

E

D

D

E

E

I

V

L

L

T

L

K

Q

V

M

E

A

L

Y

C

V

G

G

I

I

C
|
C

M
x
G

G
x
S

D

D

I

V

K
x
H

T

Y

Y

Y

R

-

G

-

A

-

P

-

S

-

F

-

W

-

G

-

D

-

A

-

E

E

Q

H

P

G

R

R

Y

I

-

A

-

D

-

F

-

I

V

V

K

K

P

D

P

P

M

M

I

V

P

I

G

G

H
|
H

E
|
E

F

A

V

S

C

G

R

T

V

V

A

K

L

V

G

G

P

K

G

N

A

V

E

-

K

-

R

K

G

H

V

L

K

K

V

K

G

G

D

D

R

R

V

V

I

A

S

V

E

E

Q

P

I

G

V

V

P

P

C
|
C

W

R

G

R

C
|
C

R

Q

F

F

C
|
C

N

K

H

E

G

G

Q

K

Y

Y

W

N

M

L

C
|
C

Q

P

K

D

H

L

D
x
T

L

F

Y

C

G

A

F

T

Q

P

N

P

N

D

V

-

Q

D

G

G

A

N

M

L

A

A

Q

R

Y

Y

M

Y

I

V

F

H

T

A

K

A

E

D

G

-

I

F

I

C

H

H

K

K

V

L

P

P

D

D

S

N

I

V

A

S

P

L

D

E

E

E

A

G

I

A

L

L

I

L

E

E

P
|
P

L

L

A

S

C

V

S
x
G

L

V

H

H

A

A

A

C

E

R

R

R

A

A

N

G

V

V

D

Q

F

L

D

G

D

T

V

T

V

V

V

L

V

V

A

I

G

G

A
|
A

G

G

T

P

L

I

G

G

L

L

G

V

I

S

I

V

G

L

A

A

V

A

R

K

M

A

R

Y

N

G

P

-

K

A

K

F

L

V

I

V

V

C

L

T

D
x
A

M
x
R

K
x
S

P

P

E

R

R
|
R

A

L

A

E

L

V

A

A

L

K

R

N

M

C

G

G

A

A

D

D

E

V

-

T

-

L

V

V

W

V

N

D

P

P

A

A

E

K

E

E

D

E

V

E

L

S

A

S

K

I

I

I

R

E

E

R

I

I

T

R

D

S

G

A

Y

I

G

G

-

D

-

L

C

P

D

N

I

V

Y

T

I

I

E

D

A

C

T

S

G

G

H

N

H

E

K

K

A

C

V

I

N

T

Q

I

G

G

L

I

A

N

M

I

L

T

R

R

K

T

L

G

G

G

R

T

F

L

V

M

E

L

F

V

S

G

V

M

F

G

N

S

D

Q

E

M

A

V

T

T

V

V

D

P

W

L

S

-

I

V

I

N

G

A

D

C

R

A

K

R

E

E

L

I

D

D

V

I

L

K

G

S

S

V

H

F

L

R

G

Y

P

C

Y

N

M

D

Y

Y

P

P

R

I

A

A

I

L

D

E

F

M

I

V

G

A

N

S

R

G

K

R

I

C

D

N

M

V

R

K

D

Q

V

L

V

V

T

T

H

H

T

S

F

F

A

K

L

L

A

E

D

Q

F

T

K

V

E

D

A

A

F

F

-

E

A

A

V

A

M

R

E

K

R

K

G

A

D

D

K

N

S

T

L

I

K
|
K

V

V

V

M

L

I

Q

S

active site: C38 (= C56), G39 (≠ M57), S40 (≠ G58), H43 (≠ K61), H63 (= H87), E64 (= E88), C93 (= C119), C96 (= C122), C99 (= C125), C107 (= C133), T111 (≠ D137), P150 (= P178), G154 (≠ S182), K341 (= K366)
binding phosphate ion: A174 (= A202), A196 (≠ D225), R197 (≠ M226), S198 (≠ K227), R201 (= R230)
binding zinc ion: C38 (= C56), H63 (= H87), E64 (= E88), C93 (= C119), C96 (= C122), C99 (= C125), C107 (= C133)

3qe3A Sheep liver sorbitol dehydrogenase (see paper)
29% identity, 94% coverage: 22:370/371 of query aligns to 5:343/351 of 3qe3A

query
sites
3qe3A

A

S

V

L

V

V

C

V

H

H

G

G

P

P

E

G

D

D

Y

L

R

R

L

L

E

E

T

N

V

Y

D

P

V

I

P

P

V

E

P

P

G

G

P

P

D

N

E

E

I

V

L

L

T

L

K

K

V

M

E

H

L

S

C

V

G

G

I

I

C
|
C

M
x
G

G
x
S

D

D

I

V

K
x
H

T

-

Y

-

R

-

G

-

A

-

P

-

S

-

F
x
Y

W

W

G

Q

D

H

A

G

E

R

Q

I

P

G

R

D

Y
x
F

V

V

-

V

K

K

P

K

P

P

M

M

I

V

P

L

G

G

H
|
H

E
|
E

F

A

V

S

C

G

R

T

V

V

A

K

L

V

G

G

P

-

G

-

A

S

E

L

K

V

R

R

G

H

V

L

K

Q

V

P

G

G

D

D

R

R

V

V

I

A

S

I

E

Q

Q

P

I

G

V

A

P

P

C
x
R

W

Q

G

T

C
x
D

R

E

F

F

C
|
C

N

K

H

I

G

G

Q

R

Y

Y

W

N

M

L

C

-

Q
x
S

K

P

H

T

D

I

L
x
F

Y

F

G

C

F

A

Q
x
T

N

P

V

D

Q

D

G

G

A

N

M

L

A

C

Q

R

Y

F

M

Y

I

K

F

H

T

N

K

A

E

N

G

-

I

F

I

C

H

Y

K

K

V

L

P

P

D

D

S

N

I

V

A

T

P

F

D

E

E

E

A

G

I

A

L

L

I

I

E

E

P
|
P

L

L

A

S

C

V

S
x
G

L

I

H

H

A

A

A

C

E

R

R

R

A

A

N

G

V

V

D

T

F

L

D

G

D

N

V

K

V

V

V

L

V

V

A

C

G

G

A

A

G

G

T

P

L

I

G

G

L

L

G

V

I

N

I

L

G

L

A

A

V

A

R

K

M

A

R

M

N

G

P

A

K

A

K

Q

L

V

I

V

V

V

L

T

D

D

M

L

K

S

P

A

E

S

R

R

A

L

A

S

L

K

A

A

L

K

R

E

M

V

G

G

A

A

D

D

-

F

-

I

-

L

E

E

V

I

W

S

N

N

P

E

A

S

E

P

E

E

D

E

V

I

L

A

A

K

K

K

I

V

R

E

E

G

I

L

T

L

D

-

G

G

Y

S

G

K

C

P

D

E

I

V

Y

T

I

I

E

E

A

C

T

T

G

G

H

V

H

E

K

T

A

S

V

I

N

Q

Q

A

G

G

L

I

A

Y

M

A

L

T

R

H

K

S

L

G

G

G

R

T

F

L

V

V

E

L

F

V

S

G

V

L

F

G

N

S

D

E

E

M

A

T

T

S

V

V

D

P

W

L

S

-

I

V

I

H

G

A

D

A

R

T

K

R

E

E

L

V

D

D

V

I

L

K

G

G

S

V

H

F

L
x
R

G

Y

P

C

Y

N

M

T

Y

W

P

P

R

M

A

A

I

I

D

S

F

M

I

L

G

A

N

S

R

K

K

S

I

V

D

N

M

V

R

K

D

P

V

L

V

V

T

T

H

H

T

R

F

F

A

P

L

L

A

E

D

K

F

A

K

L

E

E

A

A

F

F

A

E

V

T

M

S

E

K

R

K

G

G

D

-

K

L

S

G

L

L

K
|
K

V

V

V

M

L

I

Q

K

active site: C39 (= C56), G40 (≠ M57), S41 (≠ G58), H44 (≠ K61), H64 (= H87), E65 (= E88), R94 (≠ C119), D97 (≠ C122), C100 (= C125), S108 (≠ Q134), F112 (≠ L138), P151 (= P178), G155 (≠ S182), K339 (= K366)
binding glycerol: Y45 (≠ F69), F54 (≠ Y78), T116 (≠ Q142), R293 (≠ L319)
binding zinc ion: C39 (= C56), H64 (= H87), E65 (= E88)

4oh1A Crystal structure of a putative zinc-binding dehydrogenase (gutb) from clostridium scindens atcc 35704 at 2.00 a resolution
28% identity, 87% coverage: 33:355/371 of query aligns to 21:334/351 of 4oh1A

query
sites
4oh1A

L

V

E

D

T

M

V

M

D

E

V

A

P

P

V

M

P

P

G

E

P

P

-

K

-

D

D

E

E

E

I

I

L

R

T

I

K

K

V

V

E

V

L

Y

C

A

G

S

I

I

C
|
C

M
x
G

G
x
S

D

D

I

T

K
x
H

T

I

Y

L

R

T

G

G

A

N

P

-

S

-

F

-

W

L

G

G

D

E

A

M

E

E

Q

S

P

T

R

T

Y

R

V

S

K

M

P

L

P

P

M

M

I

S

P

F

G

G

H
|
H

E
|
E

F

L

V

S

C

G

R

V

V

I

V

D

A

K

L

V

G

G

P

S

G

T

A

A

E

E

K

K

R

M

G

G

V

F

K

K

V

V

G

G

D

Q

R

K

V

V

I

V

S

A

E

N

Q

Y

I

A

V

K

P

Y

C
|
C

W

G

G

C

C
|
C

R

E

F

N

C
|
C

N

R

H

E

G

G

Q

K

Y

V

W

N

M

L

C
|
C

Q

S

K

N

H

-

D

-

L

-

Y

M

G

G

F

Y

Q

R

N

M

N

N

V

-

Q

-

G

-

A

G

M

F

A

S

Q

E

Y

Y

M

A

I

V

F

Y

T

H

K

M

E

S

G

Q

I

I

I

F

H

-

K

P

V

I

P

P

D

D

S

D

I

A

A

D

P

L

D

K

E

D

A

Y

I

A

L

L

I

V

E
|
E

P
|
P

L

L

A

T

C

V

S
x
A

L

L

H

S

A

S

A

A

E

E

R

Q

A

A

N

K

V

I

D

S

F

Y

D

G

D

K

V

S

V

V

V

A

V

I

A

M

G

G

A

A

G

G

T

G

L

L

G

G

L

M

G

M

I

L

I

V

G

Q

A

L

V

A

R

R

M

L

R

A

N

G

P

A

K

S

K

T

L

I

I

T

V

V

L

F

D

D

M

I

K

V

P

P

E

E

R

K

A

L

A

E

L

L

A

A

L

L

R

E

M

N

G

G

A

A

D

D

E

Y

V

A

W

L

N

N

P

S

A

A

E

E

D

G

V

V

L

A

A

E

K

R

I

A

R

I

E

E

I

L

T

A

D

G

G

G

-

R

Y

Y

G

D

C

C

D

-

I

-

Y

V

I

L

E

E

A

G

T

T

G

G

H

A

H

T

K

A

A

A

V

A

N

K

Q

L

G

G

L

L

A

Q

M

L

L

L

R

A

K

R

L

D

G

G

R

D

F

A

V

V

E

Y

F

F

S

A

V

M

F

Y

N

G

D

K

E

D

A

P

T

I

V

L

D

P

W

V

S

N

I

L

I

Q

G

S

D

D

R

L

-

Y

-

W

K

D

E

Q

L

K

D

H

V

I

L

H

G

G

S

M

H

I

L

Q

G

G

P

A

Y

W

M

Q

Y

F

P

P

R

K

A

S

I

I

D

R

F

M

I

I

G

-

N

-

R

P

K

R

I

M

D

D

M

F

R

S

D

K

V

I

T

Q

H

K

T

E

F

H

A

T

L

L

A

T

D

N

F

Y

K

K

E

Q

A

A

F

F

active site: C46 (= C56), G47 (≠ M57), S48 (≠ G58), H51 (≠ K61), H74 (= H87), E75 (= E88), C106 (= C119), C109 (= C122), C112 (= C125), C120 (= C133), P158 (= P178), A162 (≠ S182)
binding zinc ion: C46 (= C56), H74 (= H87), E75 (= E88), C106 (= C119), C109 (= C122), C112 (= C125), C120 (= C133), E157 (= E177)

Sites not aligning to the query:

active site: 344

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 90% coverage: 36:370/371 of query aligns to 16:339/341 of P07913

query
sites
P07913

V

T

D

D

V

V

P

P

V

V

P

P

-

E

-

L

G

G

P

H

D

N

E

D

I

L

L

L

T

I

K

K

V

I

E

R

L

K

C

T

G

A

I

I

C
|
C

M

G

G

T

D

D

I

V

K

H

T

I

Y

Y

R

N

G

-

A

-

P

-

S

-

F

-

W

W

G

D

D

E

A

W

E

S

Q

Q

P

-

R

K

Y

T

V

I

K

P

P

V

P

P

M

M

I

V

P

V

G

G

H

H

E

E

F

Y

V

V

C

G

R

E

V

V

A

-

L

-

G

G

P

I

G

G

A

Q

E

E

K

V

R

K

G

G

V

F

K

K

V

I

G

G

D

D

R

R

V

V

I

S

S

G

E

E

Q

G

I

H

V

I

P

T

C

C

W

G

G

H

C

C

R

R

F

N

C

C

N

R

H

G

G

G

Q

R

Y

T

W

H

M

L

C

C

Q

R

K

-

H

-

D

N

L

T

Y

I

G

G

F

V

Q

G

N

V

N

N

V

R

Q

P

G

G

A

C

M

F

A

A

Q

E

Y

Y

M

L

I

V

F

I

T

P

K

A

E

F

G

N

I

A

I

F

H

-

K

K

V

I

P

P

D

D

S

N

I

I

A

S

P

D

D

D

E

L

A

A

I

A

L

I

I

F

E

D

P

P

L

F

A

G

C

N

S

A

L

V

H

H

A

T

A

A

E

L

R

S

A

F

N

D

V

L

D

V

F

G

D

E

D

D

V

V

V

L

V

V

V

-

A

S

G

G

A

A

G

G

T

P

L

I

G

G

L

I

G

M

I

A

G

A

A

V

V

A

R

K

M

H

R

V

N

G

P

A

K

R

K

N

L

V

I

V

V

I

L

T

D

D

M

V

K

N

P

E

E

Y

R

R

A

L

A

E

L

L

A

A

L

R

R

K

M

M

G

G

A

I

D

T

E

R

V

A

W

V

N

N

P

V

A

A

E

K

E

E

D

N

V

L

L

N

A

D

K

V

I

M

R

A

E

E

I

L

T

G

D

M

G

T

Y

E

G

G

C

F

D

D

I

V

Y

G

I

L

E

E

A

M

T

S

G

G

H

A

H

P

K

P

A

A

V

F

N

R

Q

T

G

M

L

L

A

D

M

T

L

M

R

N

K

H

L

G

G

G

R

R

F

I

V

A

E

M

F

L

S

G

V

I

F

P

N

P

D

S

E

D

A

M

T

S

V

I

D

D

W

W

S

T

I

K

I

V

G

I

D

F

R

K

K

G

E

L

L

F

-

I

-

K

D

G

V

I

L

Y

G

G

S

R

H

E

L

M

G

F

P

E

Y

T

M

W

Y

Y

P

K

R

M

A

A

I

A

D

-

F

-

I

L

G

I

N

Q

R

S

K

G

I

L

D

D

M

L

R

S

D

P

V

I

T

T

H

H

T

R

F

F

A

S

L

I

A

D

D

D

F

F

K

Q

E

K

A

G

F

F

A

D

V

A

M

M

E

R

R

S

G

G

D

-

K

Q

S

S

L

G

K

K

V

V

V

I

L

L

Q

S

C38 (= C56) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
28% identity, 94% coverage: 22:370/371 of query aligns to 11:349/357 of P27867

query
sites
P27867

A

S

V

L

V

V

C

V

H

H

G

G

P

P

E

G

D

D

Y

I

R

R

L

L

E

E

T

N

V

Y

D

P

V

I

P

P

V

E

P

L

G

G

P

P

D

N

E

D

I

V

L

L

T

L

K

K

V

M

E

H

L

S

C

V

G

G

I

I

C
|
C

M

G

G

S

D

D

I

V

K

H

T

-

Y

-

R

-

G

-

A

-

P

-

S

-

F

Y

W

W

G

E

D

H

A

G

E

R

Q

I

P

G

R

D

Y

F

V

V

-

V

K

K

P

K

P

P

M

M

I

V

P

L

G

G

H
|
H

E
|
E

F

A

V

A

C

G

R

T

V

V

V

T

A

K

L

V

G

G

P

P

G

M

A

V

E

K

K

H

R

-

G

-

V

L

K

K

V

P

G

G

D

D

R

R

V

V

I

A

S

I

E

E

Q

P

I

G

V

V

P

P

C

R

W

E

G

I

C

D

R

E

F

F

C

C

N

K

H

I

G

G

Q

R

Y

Y

W

N

M

L

C

T

Q

P

K

S

H

-

D

I

L

F

Y

F

G

C

F

A

Q

T

N

P

V

D

Q

D

G

G

A

N

M

L

A

C

Q

R

Y

F

M

Y

I

K

F

H

T

S

K

A

E

D

G

-

I

F

I

C

H

Y

K

K

V

L

P

P

D

D

S

S

I

V

A

T

P

F

D

E

E

E

A

G

I

A

L

L

I

I

E
|
E

P

P

L

L

A

S

C

V

S

G

L

I

H

Y

A

A

A

C

E

R

R

R

A

G

N

S

V

V

D

S

F

L

D

G

D

N

V

K

V

V

V

L

V

V

A

C

G

G

A

A

G

G

T

P

L

I

G

G

L

I

G

V

I

T

I

L

G

L

A

V

V

A

R

K

M

A

R

M

N

G

P

A

K

S

K

Q

L

V

I

V

V

V

L

I

D
|
D

M

L

K

S

P

A

E

S

R
|
R

A

L

A

A

L

K

A

A

L

K

R

E

M

V

G

G

A

A

D

D

E

F

V

T

W

I

N

Q

P

V

A

A

E

K

E

E

-

T

-

P

-

H

D

D

V

I

L

A

A

K

K

K

I

V

R

E

E

S

I

V

T

L

D

-

G

G

Y

S

G

K

C

P

D

E

I

V

Y

T

I

I

E

E

A

C

T

T

G

G

H

A

H

E

K

S

A

S

V

V

N

Q

Q

T

G

G

L

I

A

Y

M

A

L

T

R

H

K

S

L

G

G

G

R

T

F

L

V

V

E

V

F
x
V

S
x
G

V
x
M

F

G

N

P

D

E

E

M

A

I

T

N

V

L

D

P

W

L

S

-

I

V

I

H

G

A

D

A

R

V

K

R

E

E

L

V

D

D

V

I

L

K

G

G

S
x
V

H
x
F

L
x
R

G

Y

P

C

Y

N

M

T

Y

W

P

P

R

M

A

A

I

V

D

S

F

M

I

L

G

A

N

S

R

K

K

T

I

L

D

N

M

V

R

K

D

P

V

L

V

V

T

T

H

H

T

R

F

F

A

P

L

L

A

E

D

K

F

A

K

V

E

E

A

A

F

F

A

E

V

T

M

A

E

K

R

K

G

G

D

-

K

L

S

G

L

L

K

K

V

V

V

M

L

I

Q

K

C45 (= C56) binding Zn(2+)
H70 (= H87) binding Zn(2+)
E71 (= E88) binding Zn(2+)
E156 (= E177) binding Zn(2+)
D204 (= D225) binding NAD(+)
R209 (= R230) binding NAD(+)
VGM 273:275 (≠ FSV 292:294) binding NAD(+)
VFR 297:299 (≠ SHL 317:319) binding NAD(+)

5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
29% identity, 91% coverage: 33:369/371 of query aligns to 14:336/339 of 5kiaA

query
sites
5kiaA

L

L

E

T

T

R

V

V

D

K

V

K

P

P

V

E

P

V

G

G

P

H

D

N

E

D

I

V

L

L

T

I

K

K

V

I

E

R

L

R

C

T

G

A

I

I

C
|
C

M
x
G

G
x
T

D

D

I

I

K
x
H

T

I

Y

W

R

K

G

-

A

-

P

-

S

-

F

-

W

W

G

D

D

D

A

W

E

A

Q

Q

P

K

R

T

Y

I

V

-

K

-

P

P

P

V

M

M

I

H

P

V

G

G

H
|
H

E
|
E

F

Y

V

V

C

G

R

E

V

I

V

V

A

E

L

M

G

G

P

Q

G

-

A

-

E

E

K

V

R

R

G

G

V

F

K

S

V

I

G

G

D

D

R

R

V

V

I

S

S

G

E

E

Q

G

I

H

V

I

P

T

C
|
C

W

G

G

F

C
|
C

R

R

F

N

C
|
C

N

R

H

A

G

G

Q

R

Y

R

W

H

M

L

C
|
C

Q

R

K

-

H

-

D

N

L

T

Y
x
V

G

G

F

V

Q

G

N

V

N

N

V

R

Q

E

G

G

A

A

M

F

A

A

Q

E

Y

Y

M

L

I

A

F

I

T

P

K

A

E

F

G

N

I

A

I

F

H

-

K

K

V

I

P

P

D

P

S

E

I

I

A

S

P

D

D

D

E

L

A

A

I

A

L

I

I

F

E
x
D

P
|
P

L

F

A

G

C
x
N

S
x
A

L

T

H

H

A

T

A

A

E

L

R

S

A

F

N

N

V

L

D

V

F

G

D

E

D

D

V

V

V

L

V

I

V

T

A

-

G

G

A

A

G

G

T

P

L

I

G

G

L

V

G

M

I

A

I

V

G

A

A

I

V

A

R

K

M

H

R

V

N

G

P

A

K

R

K

N

L

V

I

V

V

I

L

T

D

D

M

I

K

N

P

D

E

Y

R

R

A

L

A

D

L

L

A

A

L

R

R

R

M

M

G

G

A

A

D

T

E

R

V

A

W

V

N

N

P

V

A

S

E

R

E

E

-

S

-

L

-

R

D

D

V

V

L

M

A

A

K

D

I

L

R

-

E

H

I

M

T

T

D

E

G

G

Y

F

G

-

C

-

D

D

I

V

Y

G

I

L

E

E

A

M

T

S

G

G

H

V

H

P

K

S

A

A

V

F

N

T

Q

S

G

L

L

L

A

E

M

S

L

M

R

N

K

H

L

G

G

G

R

K

F

V

V

A

E

L

F

L

S

G

V

I

F

P

N

P

D

A

E

Q

A

T

T

A

V

I

D

D

W

W

S

N

I

Q

I

V

G

-

D

I

R

F

K

K

E

G

L

L

D

E

V

I

L

K

G

G

S

I

H

Y

L

-

G

G

P

R

Y
x
E

M

M

Y

F

P

E

R

T

A

W

I

Y

D

K

F

M

I

V

G

A

-

M

-

L

N

Q

R

S

K

G

I

L

D

D

M

L

R

S

D

P

V

I

T

T

H

H

T

R

F

F

A

A

L

V

A

D

D

D

F

Y

K

E

E

K

A

G

F

F

A

A

V

A

M

M

E

L

R

S

G

G

D

E

K

-

S

S

L

G

K
|
K

V

V

V

I

L

L

active site: C37 (= C56), G38 (≠ M57), T39 (≠ G58), H42 (≠ K61), H61 (= H87), E62 (= E88), C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133), V109 (≠ Y139), P147 (= P178), A151 (≠ S182), K333 (= K366)
binding calcium ion: D146 (≠ E177), N150 (≠ C181), E288 (≠ Y322)
binding zinc ion: C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133)

4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
27% identity, 89% coverage: 20:348/371 of query aligns to 4:320/342 of 4ejmA

query
sites
4ejmA

M

M

Q

K

A

A

V

V

V

R

C

L

H

E

G

S

P

V

E

G

D

N

Y

I

R

S

L

V

E

R

T

N

V

V

D

G

V

I

P

P

V

E

P

P

G

G

P

P

D

D

E

D

I

L

L

L

T

V

K

K

V

V

E

E

L

A

C

C

G

G

I

I

C
|
C

M
x
G

G
x
T

D

D

I

R

K
x
H

T

L

Y

L

R

H

G

G

A

-

P

-

S

-

F

-

W

-

G

-

D

-

A

-

E

E

Q

F

P

P

R

S

Y

-

V

-

K

T

P

P

M

V

I

T

P

L

G

G

H
|
H

E
|
E

F

F

V

-

C

C

R

G

V

I

V

V

A

-

L

V

G

E

P

A

G

G

A

S

E

A

K

V

R

R

G

D

V

I

K

A

V

P

G

G

D

A

R

R

V

I

I

T

S

G

E

D

Q

P

I

N

V

I

P

S

C
|
C

W

G

G

R

C
|
C

R

P

F

Q

C
|
C

N

Q

H

A

G

G

Q

R

Y

V

W

N

M

L

C
|
C

Q

R

K

N

H

L

D
x
R

L

A

Y

I

G

G

F

I

Q

H

N

R

N

D

V

-

Q

-

G

G

A

G

M

F

A

A

Q

E

Y

Y

M

V

I

L

F

V

T

P

K

R

E

K

G

Q

I

A

I

F

H

-

K

E

V

I

P

P

D

L

S

T

I

L

A

D

P

P

D

V

E

H

A

G

I

A

L

F

I

C

E

E

P
|
P

L

L

A

A

C

C

S
x
C

L

L

H

H

A

G

A

V

E

D

R

L

A

S

N

G

V

I

D

K

F

A

D

G

D

S

V

T

V

V

V

A

V

I

A

L

G
|
G

A

G

G
|
G

T
x
V

L
x
I

G

G

L

L

G

L

I

T

I

V

G

Q

A

L

V

A

R

R

M

L

R

A

N

G

P

A

K

T

L

V

I

I

V

L

L

S

D
x
T

M
x
R

K
x
Q

P

A

E

T

R
x
K

A

R

L

L

A

A

L

E

R

E

M

V

G

G

A

A

D

T

E

A

V

T

W

V

N

D

P

P

A

S

E

A

E

G

D

D

V

V

L

V

A

E

K

A

I

I

R

A

-

G

-

P

-

V

E

G

I

L

T

V

D

P

G

G

Y

-

G

G

C

V

D

D

I

V

Y

V

I

I

E

E

A
x
C

T

A

G

G

H

V

H

A

K
x
E

A
x
T

V

V

N

K

Q

Q

G

S

L

T

A

R

M

L

L

A

R

K

K

A

L

G

G

G

R

T

F

V

V

V

E

I

F
x
L

S

G

V
|
V

F

L

N

P

D

Q

E

G

A

E

T

K

V

V

D

E

-

I

-

E

-

P

W

F

S

D

I

I

I

L

G

-

D

-

R

F

K

R

E

E

L

L

D

R

V

V

L

L

G

G

S

S

H

F

L
x
I

G

N

P

P

Y

F

M

V

Y

H

P

R

R

R

A

A

I

A

D

D

F

L

I

V

G

A

N

T

R

G

K

A

I

I

D

E

M

I

R

D

D

R

V

M

V

I

T

S

H

R

T

R

F

I

A

S

L

L

active site: C40 (= C56), G41 (≠ M57), T42 (≠ G58), H45 (≠ K61), H61 (= H87), E62 (= E88), C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133), R109 (≠ D137), P147 (= P178), C151 (≠ S182)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G201), G172 (= G203), V173 (≠ T204), I174 (≠ L205), T194 (≠ D225), R195 (≠ M226), Q196 (≠ K227), K199 (≠ R230), C240 (≠ A269), E245 (≠ K274), T246 (≠ A275), L263 (≠ F292), V265 (= V294), I291 (≠ L319)
binding zinc ion: C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133)

Sites not aligning to the query:

active site: 337

4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
27% identity, 89% coverage: 20:348/371 of query aligns to 4:320/343 of 4ej6A

query
sites
4ej6A

M

M

Q

K

A

A

V

V

V

R

C

L

H

E

G

S

P

V

E

G

D

N

Y

I

R

S

L

V

E

R

T

N

V

V

D

G

V

I

P

P

V

E

P

P

G

G

P

P

D

D

E

D

I

L

L

L

T

V

K

K

V

V

E

E

L

A

C

C

G

G

I

I

C
|
C

M
x
G

G
x
T

D

D

I

R

K
x
H

T

L

Y

L

R

H

G

G

A

-

P

-

S

-

F

-

W

-

G

-

D

-

A

-

E

E

Q

F

P

P

R

S

Y

-

V

-

K

T

P

P

M

V

I

T

P

L

G

G

H
|
H

E
|
E

F

F

V

-

C

C

R

G

V

I

V

V

A

-

L

V

G

E

P

A

G

G

A

S

E

A

K

V

R

R

G

D

V

I

K

A

V

P

G

G

D

A

R

R

V

I

I

T

S

G

E

D

Q

P

I

N

V

I

P

S

C
|
C

W

G

G

R

C
|
C

R

P

F

Q

C
|
C

N

Q

H

A

G

G

Q

R

Y

V

W

N

M

L

C
|
C

Q

R

K

N

H

L

D
x
R

L

A

Y

I

G

G

F

I

Q

H

N

R

N

D

V

-

Q

-

G

G

A

G

M

F

A

A

Q

E

Y

Y

M

V

I

L

F

V

T

P

K

R

E

K

G

Q

I

A

I

F

H

-

K

E

V

I

P

P

D

L

S

T

I

L

A

D

P

P

D

V

E

H

A

G

I

A

L

F

I

C

E

E

P
|
P

L

L

A

A

C

C

S
x
C

L

L

H

H

A

G

A

V

E

D

R

L

A

S

N

G

V

I

D

K

F

A

D

G

D

S

V

T

V

V

V

A

V

I

A

L

G

G

A

G

G

G

T

V

L

I

G

G

L

L

G

L

I

T

I

V

G

Q

A

L

V

A

R

R

M

L

R

A

N

G

P

A

K

T

L

V

I

I

V

L

L

S

D

T

M

R

K

Q

P

A

E

T

R

K

A

R

L

L

A

A

L

E

R

E

M

V

G

G

A

A

D

T

E

A

V

T

W

V

N

D

P

P

A

S

E

A

E

G

D

D

V

V

L

V

A

E

K

A

I

I

R

A

-

G

-

P

-

V

E

G

I

L

T

V

D

P

G

G

Y

-

G

G

C

V

D

D

I

V

Y

V

I

I

E

E

A

C

T

A

G

G

H

V

H

A

K

E

A

T

V

V

N

K

Q

Q

G

S

L

T

A

R

M

L

L

A

R

K

K

A

L

G

G

G

R

T

F

V

V

V

E

I

F

L

S

G

V

V

F

L

N

P

D

Q

E

G

A

E

T

K

V

V

D

E

-

I

-

E

-

P

W

F

S

D

I

I

I

L

G

-

D

-

R

F

K

R

E

E

L

L

D

R

V

V

L

L

G

G

S

S

H

F

L

I

G

N

P

P

Y

F

M

V

Y

H

P

R

R

R

A

A

I

A

D

D

F

L

I

V

G

A

N

T

R

G

K

A

I

I

D

E

M

I

R

D

D

R

V

M

V

I

T

S

H

R

T

R

F

I

A

S

L

L

active site: C40 (= C56), G41 (≠ M57), T42 (≠ G58), H45 (≠ K61), H61 (= H87), E62 (= E88), C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133), R109 (≠ D137), P147 (= P178), C151 (≠ S182)
binding zinc ion: C91 (= C119), C94 (= C122), C97 (= C125), C105 (= C133)

Sites not aligning to the query:

active site: 337

4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
27% identity, 94% coverage: 20:369/371 of query aligns to 4:334/337 of 4ilkA

query
sites
4ilkA

M

M

Q

K

A

S

V

I

V

L

C

I

H

E

G

K

P

P

E

N

D

Q

Y

L

R

S

L

I

E

I

T

E

V

R

D

E

V

I

P

P

V

T

P

P

G

S

P

A

D

G

E

E

I

V

L

R

T

V

K

K

V

V

E

K

L

L

C

A

G

G

I

I

C
|
C

M
x
G

G
x
S

D

D

I

S

K
x
H

T

I

Y

Y

R

R

G

G

A

H

P

N

S

P

F

Y

W

-

G

-

D

-

A

-

E

-

Q

-

P

-

R

-

Y

-

V

-

K

-

P

-

P

P

M

R

I

V

P

I

G

G

H
|
H

E
|
E

F

F

V

F

C

G

R

V

V

I

V

D

A

A

L

V

G

G

P

E

G

G

A

V

E

E

K

S

R

-

G

-

V

A

K

R

V

V

G

G

D

E

R

R

V

V

I

A

S

V

E

D

Q

P

I

V

V

V

P

S

C
|
C

W

G

G

H

C
|
C

R

Y

F

P

C
|
C

N

S

H

I

G

G

Q

K

Y

P

W

N

M

V

C
|
C

Q

T

K

T

H

L

D
x
A

L

V

Y

L

G

G

F

V

Q

H

N

A

N

D

V

-

Q

-

G

G

A

G

M

F

A

S

Q

E

Y

Y

M

A

I

V

F

V

T

P

K

A

E

K

G

N

I

A

I

-

H

W

K

K

V

I

P

P

D

E

S

A

I

V

A

A

P

D

D

Q

E

Y

A

A

I

V

L

M

I

I

E
|
E

P
|
P

L

F

A

T

C

I

S
x
A

L

A

H

N

A

V

A

T

E

G

R

H

A

G

N

Q

V

P

D

T

F

E

D

N

D

D

V

T

V

V

V

L

V

V

A

Y

G
|
G

A

A

G
|
G

T
x
P

L
x
I

G

G

L

L

G

T

I

I

I

V

G

Q

A

V

V

L

R

K

-

G

M

V

R

Y

N

N

P

V

K

K

K

N

L

V

I

I

V

V

L

A

D
|
D

M
x
R

K

I

P

D

E

E

R
|
R

A

L

A

E

L

K

A

A

L

K

R

E

M

S

G

G

A

A

D

D

E

W

V

A

W

I

N

N

P
x
N

A

S

E

Q

E

T

D

P

V

L

L

G

A

E

K

S

I

F

R

A

E

E

I

-

T

-

D

K

G

G

Y

I

G

K

C

P

D

T

I

L

Y

I

I

I

E

D

A
|
A

T
x
A

G
x
C

H

H

H

P

K

S

A
x
I

V

L

N

K

Q

E

G

A

L

V

A

T

M

L

L

A

R

S

K

P

L

A

G

A

R

R

F

I

V

V

E

L

F
x
M

S

G

V

-

F
|
F

N

S

D

S

E

E

A

P

T

S

-

E

-

V

V

I

D

Q

W

Q

S

G

I

I

I

T

G

G

D

-

R

-

K

K

E

E

L

L

D

S

V

I

L

F

G

S

S

S

H

R

L

L

G

N

P

A

Y

N

M

K

Y

F

P

P

R

V

A

V

I

I

D

D

F

W

I

L

G

S

N

K

R

G

K

L

I

I

D

K

M

P

R

E

D

K

V

L

V

I

T

T

H

H

T

T

F

F

A

D

L

F

A

Q

D

H

F

V

K

A

E

D

A

A

F

I

A

S

V

L

M

F

E

E

R

L

G

D

D

Q

K

K

S

H

L

C

K

C

V
x
K

V

V

L

L

active site: C40 (= C56), G41 (≠ M57), S42 (≠ G58), H45 (≠ K61), H59 (= H87), E60 (= E88), C89 (= C119), C92 (= C122), C95 (= C125), C103 (= C133), A107 (≠ D137), P145 (= P178), A149 (≠ S182), K332 (≠ V367)
binding manganese (ii) ion: C40 (= C56), H59 (= H87), E60 (= E88), E144 (= E177)
binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G201), G170 (= G203), P171 (≠ T204), I172 (≠ L205), D193 (= D225), R194 (≠ M226), R198 (= R230), N213 (≠ P245), A235 (= A269), A236 (≠ T270), C237 (≠ G271), I241 (≠ A275), M258 (≠ F292), F260 (= F295)
binding zinc ion: C89 (= C119), C92 (= C122), C95 (= C125), C103 (= C133)

Query Sequence

>GFF2362 FitnessBrowser__WCS417:GFF2362
MSTVTERTPEQLSPVIPKTMQAVVCHGPEDYRLETVDVPVPGPDEILTKVELCGICMGDI
KTYRGAPSFWGDAEQPRYVKPPMIPGHEFVCRVVALGPGAEKRGVKVGDRVISEQIVPCW
GCRFCNHGQYWMCQKHDLYGFQNNVQGAMAQYMIFTKEGIIHKVPDSIAPDEAILIEPLA
CSLHAAERANVDFDDVVVVAGAGTLGLGIIGAVRMRNPKKLIVLDMKPERAALALRMGAD
EVWNPAEEDVLAKIREITDGYGCDIYIEATGHHKAVNQGLAMLRKLGRFVEFSVFNDEAT
VDWSIIGDRKELDVLGSHLGPYMYPRAIDFIGNRKIDMRDVVTHTFALADFKEAFAVMER
GDKSLKVVLQP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory