SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2363 FitnessBrowser__Phaeo:GFF2363 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 94% coverage: 4:398/421 of query aligns to 2:390/401 of Q56YA5

query
sites
Q56YA5

D

D

V

Y

S

M

L

Y

A

G

A

P

G

G

R

R

G

H

Y

H

L

L

A

F

I

V

P

P

G

G

P

P

S

V

V

N

I

I

P

P

D

E

A

P

V

V

L

I

Q

R

A

A

M

M

H

N

R

R

P

N

S

N

P

E

N

D

I

Y

Y

R

A

S

G

P

E

A

L

I

V

P

E

A

M

L

T

T

A

K

T

T

L

L

I

L

P

E

D

D

L

V

R

K

V

I

A

F

R

K

T

T

E

T

H

S

N

G

V

T

A

P

-

F

I

L

Y

F

I

P

S

T

N
x
T

G
|
G

H
x
T

G

G

A

A

W

W

E

E

A

S

A

A

L

L

Q

T

N

N

T

T

L

L

Q

S

P

P

G

G

D

D

T

R

V

I

L

V

V

S

A

F

S

L

S

I

G

G

R

Q

F

F

A

S

I

L

G

L

W

W

S

I

E

D

M

Q

A

Q

E

K

A

R

L

L

G

N

I

F

K

N

V

V

E

D

L

V

-

E

-

S

D

D

F

W

G

G

T

Q

G

G

A

A

P

-

W

-

D

N

M

L

D

Q

R

V

I

L

A

A

S

S

H

K

L

L

A

S

A

Q

D

D

T

E

A

N

H

H

R

T

I

I

K

K

A

A

V

I

L

C

A

I

V

V

H

H

V

N

D

E

T
|
T

S

A

S

T

S

G

I

V

R

T

N

N

D

D

V

I

A

S

A

A

M

V

R

R

A

T

A

L

L

L

D

D

A

H

C

Y

D

K

H

H

P

P

A

A

L

L

M

L

A

V

D

D

C

G

I

V

A

S

S

S

L

I

G

C

C

A

D

L

R

D

F

F

E

R

M

M

D

D

A

E

W

W

G

G

V

V

D

D

V

V

M

A

V

L

A

T

A

G

C

S

Q
|
Q

K
|
K

G

A

L

L

M

S

V

L

P

P

A

T

G

G

M

L

G

G

F

I

V

V

F

C

F

A

S

S

P

P

K

K

A

A

A

L

E

E

A

A

R

T

A

K

R

T

L

S

P

K

R

S

V

L

S

K

R

V

Y

F

W

F

D

D

W

W

E

N

P

D

R

Y

A

L

N

K

P

F

E

Y

E

K

F

L

Y

G

Q

T

Y

Y

F

W

G

P

G

Y

T

T

A
x
P

P

S

T

I

H

Q

H

L

L

L

Y

Y

G

G

L

L

R

R

A

A

L

L

D

D

L

L

I

I

H

F

G

E

E

E

G

G

M

L

E

E

A

N

V

I

W

I

A

A

R

R

H

H

H

A

R

R

L

L

A

G

Q

K

A

A

I

T

W

R

A

L

A

A

C

V

D

E

R

A

W

W

G

G

D

-

G

-

P

-

L

L

R

K

M

N

N

C

V

T

Q

Q

D

K

V

E

A

E

L

W

R

I

S

S

N

N

A

T

V

V

T

T

S

A

L

V

-

M

-

V

-

P

H

H

L

I

G

D

G

G

D

S

E

E

A

I

T

V

R

R

L

-

R

R

T

A

W

W

V

-

E

-

Q

Q

T

R

L

Y

G

N

L

L

T

S

L

L

G

G

I

L

G

G

L

L

G

N

M

K

A

V

P

A

P

G

N

K

S

-

P

-

E

-

W

-

H

-

G

-

F

V

L

F

R
|
R

L

I

G

G

H

H

M

L

G

G

H

N

V

V

S

N

G

E

Q

L

M

Q

I

L

M

L

G

G

L

C

L

L

G

A

G

G

V

V

D

E

A

M

G

I

L

L

K

K

A

D

L

V

E

G

I

Y

P

P

-

V

H

M

G

G

S

S

G

G

A

V

L

A

E

A

A

A

A

S

S

T

Q

Y

V

L

TGT 68:70 (≠ NGH 69:71) binding
T148 (= T149) binding
QK 200:201 (= QK 201:202) binding
K201 (= K202) binding
P251 (≠ A252) mutation to L: Abolishes aminotransferase activity.
R347 (= R357) binding

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
36% identity, 94% coverage: 4:398/421 of query aligns to 1:389/400 of 6pk3B

query
sites
6pk3B

D

D

V

Y

S

M

L

Y

A

G

A

P

G

G

R

R

G

H

Y

H

L

L

A

F

I

V

P

P

G

G

P

P

S

V

V

N

I

I

P

P

D

E

A

P

V

V

L

I

Q

R

A

A

M

M

H

N

R

R

P

N

S

N

P

E

N

D

I

Y

Y

R

A

S

G

P

E

A

L

I

V

P

E

A

M

L

T

T

A

K

T

T

L

L

I

L

P

E

D

D

L

V

R

K

V

I

A

F

R

K

T

T

E

T

H

S

N

G

V

T

A

P

-

F

I

L

Y

F

I

P

S

T

N
x
T

G
|
G

H
x
T

G

G

A

A

W
|
W

E

E

A

S

A

A

L

L

Q

T

N

N

T

T

L

L

Q

S

P

P

G

G

D

D

T

R

V

I

L

V

V

S

A

F

S

L

S

I

G

G

R

Q

F
|
F

A

S

I

L

G

L

W

W

S

I

E

D

M

Q

A

Q

E

K

A

R

L

L

G

N

I

F

K

N

V

V

E

D

L

V

-

E

-

S

D

D

F

W

G

G

T

Q

G

G

A

A

P

-

W

-

D

N

M

L

D

Q

R

V

I

L

A

A

S

S

H

K

L

L

A

S

A

Q

D

D

T

E

A

N

H

H

R

T

I

I

K

K

A

A

V

I

L

C

A

I

V

V

H

H

V

N

D

E

T
|
T

S

A

S

T

S

G

I

V

R

T

N

N

D

D

V

I

A

S

A

A

M

V

R

R

A

T

A

L

L

L

D

D

A

H

C

Y

D

K

H

H

P

P

A

A

L

L

M

L

A

V

D
|
D

C

G

I
x
V

A

S

S

S

L

I

G

C

C

A

D

L

R

D

F

F

E

R

M

M

D

D

A

E

W

W

G

G

V

V

D

D

V

V

M

A

V

L

A

T

A

G

C

S

Q
|
Q

K
|
K

G

A

L

L

M

S

V

L

P

P

A

T

G

G

M

L

G

G

F

I

V

V

F

C

F

A

S

S

P

P

K

K

A

A

A

L

E

E

A

A

R

T

A

K

R

T

L

S

P

K

R

S

V

L

S

K

R

V

Y

F

W

F

D

D

W

W

E

N

P

D

R

Y

A

L

N

K

P

F

E

Y

E

K

F

L

Y

G

Q

T

Y

Y

F

W

G

P

G

Y

T

T

A

P

P

S

T

I

H

Q

H

L

L

L

Y

Y

G

G

L

L

R

R

A

A

L

L

D

D

L

L

I

I

H

F

G

E

E

E

G

G

M

L

E

E

A

N

V

I

W

I

A

A

R

R

H

H

H

A

R

R

L

L

A

G

Q

K

A

A

I

T

W

R

A

L

A

A

C

V

D

E

R

A

W

W

G

G

D

-

G

-

P

-

L

L

R

K

M

N

N

C

V

T

Q

Q

D

K

V

E

A

E

L

W

R

I

S

S

N

N

A

T

V

V

T

T

S

A

L

V

-

M

-

V

-

P

H

H

L

I

G

D

G

G

D

S

E

E

A

I

T

V

R

R

L

-

R

R

T

A

W

W

V

-

E

-

Q

Q

T

R

L

Y

G

N

L

L

T

S

L

L

G

G

I

L

G

G

L

L

G

N

M

K

A

V

P

A

P

G

N

K

S

-

P

-

E

-

W

-

H

-

G

-

F

V

L

F

R

R

L

I

G

G

H

H

M

L

G

G

H

N

V

V

S

N

G

E

Q

L

M

Q

I

L

M

L

G

G

L

C

L

L

G

A

G

G

V

V

D

E

A

M

G

I

L

L

K

K

A

D

L

V

E

G

I

Y

P

P

-

V

H

M

G

G

S

S

G

G

A

V

L

A

E

A

A

A

A

S

S

T

Q

Y

V

L

binding pyridoxal-5'-phosphate: T67 (≠ N69), G68 (= G70), T69 (≠ H71), W72 (= W74), F94 (= F96), T147 (= T149), D174 (= D176), V176 (≠ I178), Q199 (= Q201), K200 (= K202)

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
36% identity, 92% coverage: 10:398/421 of query aligns to 6:388/399 of 6pk1A

query
sites
6pk1A

G

G

R

R

G

H

Y

H

L

L

A

F

I

V

P

P

G

G

P

P

S

V

V

N

I

I

P

P

D

E

A

P

V

V

L

I

Q

R

A

A

M

M

H

N

R

R

P

N

S

N

P

E

N

D

I

Y

Y

R

A

S

G

P

E

A

L

I

V

P

E

A

M

L

T

T

A

K

T

T

L

L

I

L

P

E

D

D

L

V

R

K

V

I

A

F

R

K

T

T

E

T

H

S

N

G

V

T

A

P

-

F

I

L

Y

F

I

P

S

T

N
x
T

G
|
G

H
x
T

G

G

A

A

W
|
W

E

E

A

S

A

A

L

L

Q

T

N

N

T

T

L

L

Q

S

P

P

G

G

D

D

T

R

V

I

L

V

V

S

A

F

S

L

S

I

G

G

R

Q

F
|
F

A

S

I

L

G

L

W

W

S

I

E

D

M

Q

A

Q

E

K

A

R

L

L

G

N

I

F

K

N

V

V

E

D

L

V

-

E

-

S

D

D

F

W

G

G

T

Q

G

G

A

A

P

-

W

-

D

N

M

L

D

Q

R

V

I

L

A

A

S

S

H

K

L

L

A

S

A

Q

D

D

T

E

A

N

H

H

R

T

I

I

K

K

A

A

V

I

L

C

A

I

V

V

H

H

V

N

D

E

T
|
T

S

A

S

T

S

G

I

V

R

T

N

N

D

D

V

I

A

S

A

A

M

V

R

R

A

T

A

L

L

L

D

D

A

H

C

Y

D

K

H

H

P

P

A

A

L

L

M

L

A

V

D
|
D

C

G

I
x
V

A

S

S

S

L

I

G

C

C

A

D

L

R

D

F

F

E

R

M

M

D

D

A

E

W

W

G

G

V

V

D

D

V

V

M

A

V

L

A

T

A

G

C

S

Q

Q

K
|
K

G

A

L

L

M

S

V

L

P

P

A

T

G

G

M

L

G

G

F

I

V

V

F

C

F

A

S

S

P

P

K

K

A

A

A

L

E

E

A

A

R

T

A

K

R

T

L

S

P

K

R

S

V

L

S

K

R

V

Y

F

W

F

D

D

W

W

E

N

P

D

R

Y

A

L

N

K

P

F

E

Y

E

K

F

L

Y

G

Q

T

Y

Y

F

W

G

P

G

Y

T

T

A

P

P

S

T

I

H

Q

H

L

L

L

Y

Y

G

G

L

L

R

R

A

A

L

L

D

D

L

L

I

I

H

F

G

E

E

E

G

G

M

L

E

E

A

N

V

I

W

I

A

A

R

R

H

H

H

A

R

R

L

L

A

G

Q

K

A

A

I

T

W

R

A

L

A

A

C

V

D

E

R

A

W

W

G

G

D

-

G

-

P

-

L

L

R

K

M

N

N

C

V

T

Q

Q

D

K

V

E

A

E

L

W

R

I

S

S

N

N

A

T

V

V

T

T

S

A

L

V

-

M

-

V

-

P

H

H

L

I

G

D

G

G

D

S

E

E

A

I

T

V

R

R

L

-

R

R

T

A

W

W

V

-

E

-

Q

Q

T

R

L

Y

G

N

L

L

T

S

L

L

G

G

I

L

G

G

L

L

G

N

M

K

A

V

P

A

P

G

N

K

S

-

P

-

E

-

W

-

H

-

G

-

F

V

L

F

R
|
R

L

I

G

G

H

H

M

L

G

G

H

N

V

V

S

N

G

E

Q

L

M

Q

I

L

M

L

G

G

L

C

L

L

G

A

G

G

V

V

D

E

A

M

G

I

L

L

K

K

A

D

L

V

E

G

I

Y

P

P

-

V

H

M

G

G

S

S

G

G

A

V

L

A

E

A

A

A

A

S

S

T

Q

Y

V

L

binding 3-hydroxypyruvic acid: F93 (= F96), R345 (= R357)
binding pyridoxal-5'-phosphate: T66 (≠ N69), G67 (= G70), T68 (≠ H71), W71 (= W74), F93 (= F96), T146 (= T149), D173 (= D176), V175 (≠ I178), K199 (= K202)

6mfbD Crystal structure of 3-hydroxykynurenine transaminase from aedes aegypti
30% identity, 72% coverage: 17:318/421 of query aligns to 24:322/386 of 6mfbD

query
sites
6mfbD

P

P

G

G

P

P

S

S

V

N

I

C

P

S

D

K

A

R

V

V

L

L

Q

A

A

A

M

L

H

N

R

N

P

T

S

C

P

L

N

S

I

N

Y

F

A

H

G

D

E

E

L

L

V

F

E

Q

M

V

T

I

A

D

T

E

L

V

I

K

P

D

D

G

L

L

R

R

R

Y

V

I

A

F

R

Q

T

T

E

E

H

N

N

R

V

T

A

T

I

M

Y

C

I

I

S

T

-

G

N
x
S

G
x
A

H
|
H

G

T

A

G

W

M

E

E

A

A

A

L

L

L

Q

C

N

N

T

L

L

L

Q

E

P

E

G

G

D

D

T

I

V

V

L

L

V

I

A

A

S

N

G

G

R

I

F
x
W

A

A

I

E

G

R

W

A

S

I

E

N

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

L

L

D

E

F

G

G

P

T

A

G

D

A

K

P

P

W

F

D

S

M

M

D

T

R

D

I

F

A

K

S

K

H

-

L

-

A

-

A

A

D

I

T

E

A

Q

H

H

R

R

I

P

K

K

A

C

V

L

L

F

A

V

V

V

H

H

V

G

D

D

T
x
S

S

S

S

G

I

L

R

L

N

Q

D

P

V

L

A

E

A

G

M

L

R

G

A

K

A

I

L

C

D

H

A

-

C

-

D

D

H

Y

P

D

A

C

L

L

M

L

A

V

D
|
D

C

A

I
x
V

A

A

S

S

L

L

G

C

C

G

D

V

R

P

F

F

E

Y

M

M

D

D

A

K

W

W

G

E

V

I

D

D

V

G

M

V

V

Y

A

T

A

G

C

S

Q
|
Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

I

F

S

F

I

S

S

P

P

K

K

A

A

A

L

E

E

A

V

-

I

R

R

A

S

R

R

-

K

L

T

P

P

R

S

V

K

S

V

R

F

Y

Y

W

W

D

D

W

L

E

L

P

I

R

L

A

G

N

N

P

Y

E

W

E

G

F

C

Y

Y

-

D

-

E

-

Q

Q

K

Y

R

F
x
Y

G

H

T
|
T

A

V

P

P

T

S

H

N

H

L

L

I

Y

F

G

A

L

L

R

R

A

E

A

A

L

I

D

A

L

Q

I

I

H

A

G

E

E

E

G

G

M

L

E

E

A

P

V

V

W

I

A

R

R

R

H

R

H

Q

R

E

L

C

A

A

Q

E

A

Q

I

M

W

Y

A

R

A

G

C

L

D

Q

R

A

W

M

G

G

D

-

G

-

P

-

L

L

R

E

M

I

N

F

V

V

Q

K

D

D

V

P

A

E

L

Y

R

R

S

L

N

P

A

T

V

V

T

T

S

C

L

I

H

M

L

I

binding pyridoxal-5'-phosphate: S77 (≠ N69), A78 (≠ G70), H79 (= H71), W104 (≠ F96), S154 (≠ T149), D179 (= D176), V181 (≠ I178), Q204 (= Q201), K205 (= K202), Y256 (≠ F248), T259 (= T251)

Q0IG34 3-hydroxykynurenine transaminase; 3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase; Ae-HKT/AGT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
30% identity, 72% coverage: 17:318/421 of query aligns to 24:322/400 of Q0IG34

query
sites
Q0IG34

P

P

G

G

P

P

S

S

V

N

I

C

P

S

D

K

A

R

V

V

L

L

Q

A

A

A

M

L

H

N

R

N

P

T

S

C

P

L

N

S

I

N

Y

F

A

H

G

D

E

E

L

L

V

F

E

Q

M

V

T

I

A

D

T

E

L

V

I

K

P

D

D

G

L

L

R

R

R

Y

V

I

A

F

R

Q

T

T

E

E

H

N

N

R

V

T

A

T

I

M

Y

C

I

I

S

T

-

G

N
x
S

G
x
A

H
|
H

G

T

A

G

W

M

E

E

A

A

A

L

L

L

Q

C

N

N

T

L

L

L

Q

E

P

E

G

G

D

D

T

I

V

V

L

L

V

I

A

A

S

N

G

G

R

I

F

W

A

A

I

E

G

R

W

A

S

I

E

N

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

L

L

D

G

F

G

G

P

T

A

G

D

A

K

P

P

W

F

D

S

M

M

D

T

R

D

I

F

A

K

S

K

H

-

L

-

A

-

A

A

D

I

T

E

A

Q

H

H

R

R

I

P

K

K

A

C

V

L

L

F

A

V

V

V

H

H

V

G

D

D

T
x
S

S

S

S

G

I

L

R

L

N

Q

D

P

V

L

A

E

A

G

M

L

R

G

A

K

A

I

L

C

D

H

A

-

C

-

D

D

H

Y

P

D

A

C

L

L

M

L

A

V

D

D

C

A

I

V

A

A

S

S

L

L

G

C

C

G

D

V

R

P

F

F

E

Y

M

M

D

D

A

K

W

W

G

E

V

I

D

D

V

G

M

V

V

Y

A

T

A

G

C

S

Q
|
Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

I

F

S

F

I

S

S

P

P

K

K

A

A

A

L

E

E

A

V

-

I

R

R

A

S

R

R

-

K

L

T

P

P

R

S

V

K

S

V

R

F

Y

Y

W

W

D

D

W

L

E

L

P

I

R

L

A

G

N

N

P

Y

E

W

E

G

F

C

Y

Y

-

D

-

E

-

Q

Q

K

Y

R

F
x
Y

G

H

T
|
T

A

V

P

P

T

S

H

N

H

L

L

I

Y

F

G

A

L

L

R

R

A

E

A

A

L

I

D

A

L

Q

I

I

H

A

G

E

E

E

G

G

M

L

E

E

A

P

V

V

W

I

A

R

R

R

H

R

H

Q

R

E

L

C

A

A

Q

E

A

Q

I

M

W

Y

A

R

A

G

C

L

D

Q

R

A

W

M

G

G

D

-

G

-

P

-

L

L

R

E

M

I

N

F

V

V

Q

K

D

D

V

P

A

E

L

Y

R

R

S

L

N

P

A

T

V

V

T

T

S

C

L

I

H

M

L

I

SAH 77:79 (≠ NGH 69:71) binding in other chain
S154 (≠ T149) binding in other chain
Q204 (= Q201) binding in other chain
K205 (= K202) modified: N6-(pyridoxal phosphate)lysine
Y256 (≠ F248) binding
T259 (= T251) binding

2ch1A Structure of anopheles gambiae 3-hydroxykynurenine transaminase (see paper)
28% identity, 87% coverage: 17:382/421 of query aligns to 23:381/388 of 2ch1A

query
sites
2ch1A

P

P

G

G

P

P

S

S

V

N

I

C

P

S

D

K

A

R

V

V

L

L

Q

T

A

A

M

M

H

T

R

N

P

T

S

V

P

L

N

S

I

N

Y

F

A

H

G

A

E

E

L

L

V

F

E

R

M

T

T

M

A

D

T

E

L

V

I

K

P

D

D

G

L

L

R

R

R

Y

V

I

A

F

R

Q

T

T

E

E

H

N

N

R

V

A

A

T

I

M

Y

C

I

V

S

S

-

G

N
x
S

G
x
A

H
|
H

G

A

A

G

W

M

E

E

A

A

A

M

L

L

Q

S

N

N

T

L

L

L

Q

E

P

E

G

G

D

D

T

R

V

V

L

L

V

I

A

A

S

V

S

N

G

G

R

I

F
x
W

A

A

I

E

G

R

W

A

S

V

E

E

M

M

A

S

E

E

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

T

L

I

D

E

F

G

G

P

T

P

G

D

A

R

P

P

W

F

D

S

M

L

D

E

R

T

I

L

A

A

S

R

H

-

L

-

A

-

A

A

D

I

T

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

L

F

A

L

V

T

H

H

V

G

D

D

T
x
S

S

S

S

G

I

L

R

-

N

-

D

-

V

L

A

Q

A

P

M

L

R

E

A

G

A

V

L

G

D

Q

A

I

C

C

-

H

D

Q

H

H

P

D

A

C

L

L

M

I

A

V

D
|
D

C

A

I
x
V

A

A

S

S

L

L

G

C

C

G

D

V

R

P

F

F

E

Y

M

M

D

D

A

K

W

W

G

E

V

I

D

D

V

A

M

V

V

Y

A

T

A

G

C

A

Q
|
Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

I

F

S

F

I

S

S

P

P

K

K

A

A

A

L

E

D

A

V

-

I

-

R

R

N

A

R

R

R

L

T

P

K

R

S

V

K

S

V

R

F

Y

Y

W

W

D

D

-

L

-

G

-

N

-

Y

W

W

E

G

P

C

R

Y

A

D

N

E

P

P

E

K

E

R

F
x
Y

Y

H

Q

H

Y

-

F

-

G

-

T
|
T

A

V

P

A

T

S

H

N

H

L

L

I

Y

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

L

Q

I

I

H

A

G

E

E

E

G

G

M

L

E

E

A

N

V

Q

W

I

A

K

R

R

H

R

H

I

R

E

L

C

A

A

Q

Q

A

I

I

L

W

Y

A

E

A

G

C

L

D

G

R

K

W

M

G

G

D

-

G

-

P

-

L

L

R

D

M

I

N

F

V

V

Q

K

D

D

V

P

A

R

L

H

R

R

S

L

N

P

A

T

V

V

T

T

S

G

L

I

H

M

L

I

-

P

G

K

G

G

D

V

E

D

A

W

T

W

R

K

L

V

R

S

T

Q

W

Y

V

A

E

M

Q

N

T

N

L

F

G

S

L

L

T

E

L

V

G

Q

I

G

G

G

L

L

G

G

M

-

A

-

P

P

P

T

N

F

S

G

P

K

E

A

W

W

H

-

G

-

F

-

L

-

R

R

L

V

G

G

H

I

M

M

G

G

H

E

V

C

S

S

G

T

-

V

Q

Q

M

K

I

I

M

Q

G

F

L

Y

L

L

G

Y

G

G

V

F

D

K

A

E

G

S

L

L

K

K

A

A

binding pyridoxal-5'-phosphate: S76 (≠ N69), A77 (≠ G70), H78 (= H71), W103 (≠ F96), S153 (≠ T149), D178 (= D176), V180 (≠ I178), Q203 (= Q201), K204 (= K202), Y255 (≠ F244), T258 (= T251)

2ch2A Structure of the anopheles gambiae 3-hydroxykynurenine transaminase in complex with inhibitor (see paper)
28% identity, 87% coverage: 17:382/421 of query aligns to 22:380/387 of 2ch2A

query
sites
2ch2A

P

P

G
|
G

P

P

S

S

V

N

I

C

P

S

D

K

A

R

V

V

L

L

Q

T

A

A

M

M

H

T

R

N

P

T

S

V

P

L

N
x
S

I
x
N

Y

F

A

H

G

A

E

E

L

L

V

F

E

R

M

T

T

M

A

D

T

E

L

V

I

K

P

D

D

G

L

L

R

R

R

Y

V

I

A

F

R

Q

T

T

E

E

H

N

N

R

V

A

A

T

I

M

Y

C

I

V

S

S

-

G

N
x
S

G
x
A

H
|
H

G

A

A

G

W

M

E

E

A

A

A

M

L

L

Q

S

N

N

T

L

L

L

Q

E

P

E

G

G

D

D

T

R

V

V

L

L

V

I

A

A

S

V

S

N

G

G

R

I

F
x
W

A

A

I

E

G

R

W

A

S

V

E

E

M

M

A

S

E

E

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

T

L

I

D

E

F

G

G

P

T

P

G

D

A

R

P

P

W

F

D

S

M

L

D

E

R

T

I

L

A

A

S

R

H

-

L

-

A

-

A

A

D

I

T

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

L

F

A

L

V

T

H

H

V

G

D

D

T
x
S

S

S

S

G

I

L

R

-

N

-

D

-

V

L

A

Q

A

P

M

L

R

E

A

G

A

V

L

G

D

Q

A

I

C

C

-

H

D

Q

H

H

P

D

A

C

L

L

M

I

A

V

D
|
D

C

A

I
x
V

A

A

S

S

L

L

G

C

C

G

D

V

R

P

F

F

E

Y

M

M

D

D

A

K

W

W

G

E

V

I

D

D

V

A

M

V

V

Y

A

T

A

G

C

A

Q

Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

I

F

S

F

I

S

S

P

P

K

K

A

A

A

L

E

D

A

V

-

I

-

R

R

N

A

R

R

R

L

T

P

K

R

S

V

K

S

V

R

F

Y

Y

W

W

D

D

-

L

-

G

-

N

-

Y

W

W

E

G

P

C

R

Y

A

D

N

E

P

P

E

K

E

R

F
x
Y

Y

H

Q

H

Y

-

F

-

G

-

T
|
T

A

V

P

A

T

S

H

N

H

L

L

I

Y

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

L

Q

I

I

H

A

G

E

E

E

G

G

M

L

E

E

A

N

V

Q

W

I

A

K

R

R

H

R

H

I

R

E

L

C

A

A

Q

Q

A

I

I

L

W

Y

A

E

A

G

C

L

D

G

R

K

W

M

G

G

D

-

G

-

P

-

L

L

R

D

M

I

N

F

V

V

Q

K

D

D

V

P

A

R

L

H

R

R

S

L

N

P

A

T

V

V

T

T

S

G

L

I

H

M

L

I

-

P

G

K

G

G

D

V

E

D

A

W

T

W

R

K

L

V

R

S

T

Q

W

Y

V

A

E

M

Q

N

T

N

L

F

G

S

L

L

T

E

L

V

G
x
Q

I

G

G

G

L
|
L

G

G

M

-

A

-

P

P

P

T

N

F

S

G

P

K

E

A

W

W

H

-

G

-

F

-

L

-

R
|
R

L

V

G

G

H

I

M

M

G

G

H

E

V

C

S

S

G

T

-

V

Q

Q

M

K

I

I

M

Q

G

F

L

Y

L

L

G

Y

G

G

V

F

D

K

A

E

G

S

L

L

K

K

A

A

binding 4-(2-aminophenyl)-4-oxobutanoic acid: G23 (= G18), S41 (≠ N36), N42 (≠ I37), S152 (≠ T149), Y254 (≠ F244), Q342 (≠ G339), L345 (= L342), R354 (= R357)
binding pyridoxal-5'-phosphate: S75 (≠ N69), A76 (≠ G70), H77 (= H71), W102 (≠ F96), S152 (≠ T149), D177 (= D176), V179 (≠ I178), K203 (= K202), Y254 (≠ F244), T257 (= T251)

Q7PRG3 3-hydroxykynurenine transaminase; AgHKT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Anopheles gambiae (African malaria mosquito) (see paper)
28% identity, 87% coverage: 17:382/421 of query aligns to 24:382/396 of Q7PRG3

query
sites
Q7PRG3

P

P

G

G

P

P

S

S

V

N

I

C

P

S

D

K

A

R

V

V

L

L

Q

T

A

A

M

M

H

T

R

N

P

T

S

V

P

L

N

S

I

N

Y

F

A

H

G

A

E

E

L

L

V

F

E

R

M

T

T

M

A

D

T

E

L

V

I

K

P

D

D

G

L

L

R

R

R

Y

V

I

A

F

R

Q

T

T

E

E

H

N

N

R

V

A

A

T

I

M

Y

C

I

V

S

S

-

G

N
x
S

G
x
A

H
|
H

G

A

A

G

W

M

E

E

A

A

A

M

L

L

Q

S

N

N

T

L

L

L

Q

E

P

E

G

G

D

D

T

R

V

V

L

L

V

I

A

A

S

V

S

N

G

G

R

I

F

W

A

A

I

E

G

R

W

A

S

V

E

E

M

M

A

S

E

E

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

T

L

I

D

E

F

G

G

P

T

P

G

D

A

R

P

P

W

F

D

S

M

L

D

E

R

T

I

L

A

A

S

R

H

-

L

-

A

-

A

A

D

I

T

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

L

F

A

L

V

T

H

H

V

G

D

D

T
x
S

S

S

S

G

I

L

R

-

N

-

D

-

V

L

A

Q

A

P

M

L

R

E

A

G

A

V

L

G

D

Q

A

I

C

C

-

H

D

Q

H

H

P

D

A

C

L

L

M

I

A

V

D

D

C

A

I

V

A

A

S

S

L

L

G

C

C

G

D

V

R

P

F

F

E

Y

M

M

D

D

A

K

W

W

G

E

V

I

D

D

V

A

M

V

V

Y

A

T

A

G

C

A

Q
|
Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

I

F

S

F

I

S

S

P

P

K

K

A

A

A

L

E

D

A

V

-

I

-

R

R

N

A

R

R

R

L

T

P

K

R

S

V

K

S

V

R

F

Y

Y

W

W

D

D

-

L

-

G

-

N

-

Y

W

W

E

G

P

C

R

Y

A

D

N

E

P

P

E

K

E

R

F
x
Y

Y

H

Q

H

Y

-

F

-

G

-

T
|
T

A

V

P

A

T

S

H

N

H

L

L

I

Y

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

L

Q

I

I

H

A

G

E

E

E

G

G

M

L

E

E

A

N

V

Q

W

I

A

K

R

R

H

R

H

I

R

E

L

C

A

A

Q

Q

A

I

I

L

W

Y

A

E

A

G

C

L

D

G

R

K

W

M

G

G

D

-

G

-

P

-

L

L

R

D

M

I

N

F

V

V

Q

K

D

D

V

P

A

R

L

H

R

R

S

L

N

P

A

T

V

V

T

T

S

G

L

I

H

M

L

I

-

P

G

K

G

G

D

V

E

D

A

W

T

W

R

K

L

V

R

S

T

Q

W

Y

V

A

E

M

Q

N

T

N

L

F

G

S

L

L

T

E

L

V

G

Q

I

G

G

G

L

L

G

G

M

-

A

-

P

P

P

T

N

F

S

G

P

K

E

A

W

W

H

-

G

-

F

-

L

-

R

R

L

V

G

G

H

I

M

M

G

G

H

E

V

C

S

S

G

T

-

V

Q

Q

M

K

I

I

M

Q

G

F

L

Y

L

L

G

Y

G

G

V

F

D

K

A

E

G

S

L

L

K

K

A

A

SAH 77:79 (≠ NGH 69:71) binding in other chain
S154 (≠ T149) binding in other chain
Q204 (= Q201) binding in other chain
K205 (= K202) modified: N6-(pyridoxal phosphate)lysine
Y256 (≠ F244) binding
T259 (= T251) binding

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
27% identity, 82% coverage: 17:362/421 of query aligns to 25:362/387 of 1j04A

query
sites
1j04A

P
|
P

G

G

P

P

S

S

V

N

I

L

P

P

D

P

A

R

V

I

L

M

Q

A

A

A

M

G

H

G

R

L

P

Q

S

M

P

I

N

G

I

S

Y

M

A

S

G

K

E

D

L

M

V

Y

E

Q

M

I

T

M

A

D

T

E

L

I

I

K

P

E

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

I

S

S

-

G

N

S

G

G

H

H

G

C

A

A

W

L

E

E

A

A

L

L

Q

V

N

N

T

V

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

V

A

G

S

A

S

N

G

G

R

I

F

W

A

G

I

Q

G

R

W

A

S

V

E

D

M

I

A

G

E

E

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

P

L

M

D

T

F

K

G

D

T

P

G

G

A

G

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

L

F

A

L

V

T

H

H

V

G

D

E

T

S

S

S

S

T

S

G

I

V

R

L

N

Q

D

P

V

L

A

D

A

G

M

F

R

R

A

-

L

-

D

E

A

L

C

C

D

H

-

R

H

Y

P

K

A

C

L

L

M

L

A

V

D

D

C

S

I

V

A

A

S

S

L

L

G

G

C

G

D

T

R

P

F

L

E

Y

M

M

D

D

A

R

W

Q

G

G

V

I

D

D

V

I

M

L

V

Y

A

S

A

G

C

S

Q

Q

K

K

G

A

L

L

M

N

V

A

P

P

A

P

G

G

M

T

G

S

F

L

V

I

F

S

F

F

S

S

P

D

K

K

A

A

A

K

E

K

-

K

-

M

-

Y

A

S

R

R

A

K

R

T

L

K

P

P

R

-

V

F

S

S

R

F

Y

Y

W

L

D

D

W

I

E

K

P

W

R

L

A

A

N

N

-

F

-

W

-

G

P

C

E

D

F

Q

Y

P

Q

R

Y

M

F

Y

G

H

T

T

A

I

P

P

T

V

H

I

H

S

L

L

Y

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I

I

H

A

G

E

E

Q

G

G

M

L

E

E

A

N

V

S

W

W

A

R

R

Q

H

H

H

R

R

E

L

A

A

A

Q

A

A

Y

I

L

W

H

A

G

A

R

C

L

D

Q

R

A

W

L

G

G

D

-

G

-

P

-

L

L

R

Q

M

L

N

F

V

V

Q

K

D

D

V

P

A

A

L

L

R

R

S

L

N

P

A

T

V

V

T

T

S

T

L

V

H

A

L

V

-

P

G

A

G

G

D

Y

E

D

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E

I

Q

D

T

H

L

F

G

D

L

I

T

E

L

I

G

M

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

S

W

T

H

G

G

K

F

V

L

L

R
|
R

L

I

G

G

H

L

M

L

G

G

binding (aminooxy)acetic acid: P25 (= P17), R357 (= R357)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
30% identity, 64% coverage: 17:287/421 of query aligns to 25:296/393 of Q3LSM4

query
sites
Q3LSM4

P

P

G

G

P

P

S

S

V

N

I

A

P

P

D

Q

A

R

V

V

L

L

Q

D

A

A

M

M

H

S

R

R

P

P

S

-

P

-

N

-

I

-

Y

I

A

L

G

G

E

H

L

L

V

H

E

P

M

E

T

T

A

L

T

K

L

I

I

M

P

D

D

D

L

I

R

K

R

E

V

G

A

V

R

R

-

Y

-

L

-

F

T

Q

E

T

H

N

N

N

V

I

A

A

I

T

Y

F

I

C

-

L

-

S

S

A

N
x
S

G
|
G

H
|
H

G

G

A

G

W

M

E

E

A

A

A

T

L

L

Q

C

N

N

T

L

L

L

Q

E

P

D

G

G

D

D

T

V

V

I

L

L

V

I

A

G

S

H

S

T

G

G

R

H

F

W

A

G

I

D

G

R

W

S

S

A

E

D

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

D

K

F

S

G

K

T

V

G

G

A

Q

P

S

W

L

D

S

M

L

D

D

R

E

I

I

A

R

S

D

H

A

L

L

A

-

D

-

T

L

A

I

H

H

R

K

I

P

K

S

A

V

V

L

L

F

A

L

V

T

H

Q

V

G

D

D

T
x
S

S

S

S

T

S

G

I

V

R

L

N

Q

D

G

V

L

A

E

A

G

M

V

R

G

A

A

A

L

L

C

D

H

A

-

C

-

D

Q

H

H

P

N

A

C

L

L

M

I

A

V

D

D

C

T

I

V

A

A

S

S

L

L

G

G

C

G

D

A

R

P

F

M

E

F

M

M

D

D

A

R

W

W

G

E

V

I

D

D

V

A

M

M

V

Y

A

T

A

G

C

S

Q
|
Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

V

F

S

F

F

S

S

P

H

K

R

A

A

A

V

E

E

A

R

R

Y

A

K

R

R

L

R

P

N

R

T

V

K

S

V

R

K

Y

V

W

Y

D

Y

W

W

E

D

P

-

R

M

A

S

N

L

P

V

E

G

E

D

F

Y

Y

W

Q

G

Y

C

F

F

G

G

G

R

T

P

A

R

P

I

T
x
Y

H

H

-
x
T

-

I

-

S

-

T

-

L

L

L

Y

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

L

M

I

A

H

C

G

E

E

E

G

G

M

L

E

P

A

A

V

L

W

I

A

A

R

R

H

H

H

E

R

D

L

C

A

A

Q

K

A

R

I

L

W

Y

SGH 78:80 (≠ NGH 69:71) binding in other chain
S155 (≠ T149) binding ; binding
Q205 (= Q201) binding in other chain
K206 (= K202) modified: N6-(pyridoxal phosphate)lysine
Y257 (≠ T254) binding
T260 (vs. gap) binding

Sites not aligning to the query:

356 binding

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
30% identity, 64% coverage: 17:287/421 of query aligns to 25:296/385 of 2huuA

query
sites
2huuA

P
|
P

G

G

P

P

S

S

V

N

I

A

P

P

D

Q

A

R

V

V

L

L

Q

D

A

A

M

M

H

S

R

R

P

P

S

-

P

-

N

-

I

-

Y

I

A

L

G

G

E
x
H

L

L

V

H

E

P

M

E

T

T

A

L

T

K

L

I

I

M

P

D

D

D

L

I

R

K

R

E

V

G

A

V

R

R

-

Y

-

L

-

F

T

Q

E

T

H

N

N

N

V

I

A

A

I

T

Y

F

I

C

-

L

-

S

S

A

N

S

G

G

H

H

G

G

A

G

W

M

E

E

A

A

A

T

L

L

Q

C

N

N

T

L

L

L

Q

E

P

D

G

G

D

D

T

V

V

I

L

L

V

I

A

G

S

H

S

T

G

G

R

H

F

W

A

G

I

D

G

R

W

S

S

A

E

D

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

D

K

F

S

G

K

T

V

G

G

A

Q

P

S

W

L

D

S

M

L

D

D

R

E

I

I

A

R

S

D

H

A

L

L

A

-

D

-

T

L

A

I

H

H

R

K

I

P

K

S

A

V

V

L

L

F

A

L

V

T

H

Q

V

G

D

D

T
x
S

S

S

S

T

S

G

I

V

R

L

N

Q

D

G

V

L

A

E

A

G

M

V

R

G

A

A

A

L

L

C

D

H

A

-

C

-

D

Q

H

H

P

N

A

C

L

L

M

I

A

V

D

D

C

T

I

V

A

A

S

S

L

L

G

G

C

G

D

A

R

P

F

M

E

F

M

M

D

D

A

R

W

W

G

E

V

I

D

D

V

A

M

M

V

Y

A

T

A

G

C

S

Q

Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

V

F

S

F

F

S

S

P

H

K

R

A

A

A

V

E

E

A

R

R

Y

A

K

R

R

L

R

P

N

R

T

V

K

S

V

R

K

Y

V

W

Y

D

Y

W

W

E

D

P

-

R

M

A

S

N

L

P

V

E

G

E

D

F

Y

Y

W

Q

G

Y

C

F

F

G

G

G

R

T

P

A

R

P

I

T
x
Y

H

H

-

T

-

I

-

S

-

T

-

L

L

L

Y

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

L

M

I

A

H

C

G

E

E

E

G

G

M

L

E

P

A

A

V

L

W

I

A

A

R

R

H

H

H

E

R

D

L

C

A

A

Q

K

A

R

I

L

W

Y

binding 1-butanol: H45 (≠ E41), Y257 (≠ T254)
binding alanine: P25 (= P17), S155 (≠ T149), K206 (= K202)

Sites not aligning to the query:

binding alanine: 347, 356

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
30% identity, 64% coverage: 17:287/421 of query aligns to 25:296/385 of 2huiA

query
sites
2huiA

P
|
P

G

G

P

P

S

S

V

N

I

A

P

P

D

Q

A

R

V

V

L

L

Q

D

A

A

M

M

H

S

R

R

P

P

S

-

P

-

N

-

I

-

Y

I

A

L

G

G

E

H

L

L

V

H

E

P

M

E

T

T

A

L

T

K

L

I

I

M

P

D

D

D

L

I

R

K

R

E

V

G

A

V

R

R

-

Y

-

L

-

F

T

Q

E

T

H

N

N

N

V

I

A

A

I

T

Y

F

I

C

-

L

-

S

S

A

N

S

G

G

H

H

G

G

A

G

W

M

E

E

A

A

A

T

L

L

Q

C

N

N

T

L

L

L

Q

E

P

D

G

G

D

D

T

V

V

I

L

L

V

I

A

G

S

H

S

T

G

G

R

H

F

W

A

G

I

D

G

R

W

S

S

A

E

D

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

D

K

F

S

G

K

T

V

G

G

A

Q

P

S

W

L

D

S

M

L

D

D

R

E

I

I

A

R

S

D

H

A

L

L

A

-

D

-

T

L

A

I

H

H

R

K

I

P

K

S

A

V

V

L

L

F

A

L

V

T

H

Q

V

G

D

D

T
x
S

S

S

S

T

S

G

I

V

R

L

N

Q

D

G

V

L

A

E

A

G

M

V

R

G

A

A

A

L

L

C

D

H

A

-

C

-

D

Q

H

H

P

N

A

C

L

L

M

I

A

V

D

D

C

T

I

V

A

A

S

S

L

L

G

G

C

G

D

A

R

P

F

M

E

F

M

M

D

D

A

R

W

W

G

E

V

I

D

D

V

A

M

M

V

Y

A

T

A

G

C

S

Q

Q

K
|
K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

V

F

S

F

F

S

S

P

H

K

R

A

A

A

V

E

E

A

R

R

Y

A

K

R

R

L

R

P

N

R

T

V

K

S

V

R

K

Y

V

W

Y

D

Y

W

W

E

D

P

-

R

M

A

S

N

L

P

V

E

G

E

D

F

Y

Y

W

Q

G

Y

C

F

F

G

G

G

R

T

P

A

R

P

I

T

Y

H

H

-

T

-

I

-

S

-

T

-

L

L

L

Y

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

L

M

I

A

H

C

G

E

E

E

G

G

M

L

E

P

A

A

V

L

W

I

A

A

R

R

H

H

H

E

R

D

L

C

A

A

Q

K

A

R

I

L

W

Y

binding glyoxylic acid: P25 (= P17), S155 (≠ T149), K206 (= K202)

Sites not aligning to the query:

binding glyoxylic acid: 356

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
30% identity, 64% coverage: 17:287/421 of query aligns to 25:296/385 of 2hufA

query
sites
2hufA

P

P

G

G

P

P

S

S

V

N

I

A

P

P

D

Q

A

R

V

V

L

L

Q

D

A

A

M

M

H

S

R

R

P

P

S

-

P

-

N

-

I

-

Y

I

A

L

G

G

E
x
H

L

L

V

H

E

P

M

E

T

T

A

L

T

K

L

I

I

M

P

D

D

D

L

I

R

K

R

E

V

G

A

V

R

R

-

Y

-

L

-

F

T

Q

E

T

H

N

N

N

V

I

A

A

I

T

Y

F

I

C

-

L

-

S

S

A

N

S

G

G

H

H

G

G

A

G

W

M

E

E

A

A

A

T

L

L

Q

C

N

N

T

L

L

L

Q

E

P

D

G

G

D

D

T

V

V

I

L

L

V

I

A

G

S

H

S

T

G

G

R

H

F

W

A

G

I

D

G

R

W

S

S

A

E

D

M

M

A

A

E

T

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

V

D

K

F

S

G

K

T

V

G

G

A

Q

P

S

W

L

D

S

M

L

D

D

R

E

I

I

A

R

S

D

H

A

L

L

A

-

D

-

T

L

A

I

H

H

R

K

I

P

K

S

A

V

V

L

L

F

A

L

V

T

H

Q

V

G

D

D

T
x
S

S

S

S

T

S

G

I

V

R

L

N

Q

D

G

V

L

A

E

A

G

M

V

R

G

A

A

A

L

L

C

D

H

A

-

C

-

D

Q

H

H

P

N

A

C

L

L

M

I

A

V

D

D

C

T

I

V

A

A

S

S

L

L

G

G

C

G

D

A

R

P

F

M

E

F

M

M

D

D

A

R

W

W

G

E

V

I

D

D

V

A

M

M

V

Y

A

T

A

G

C

S

Q

Q

K

K

G

V

L

L

M

G

V

A

P

P

A

P

G

G

M

I

G

T

F

P

V

V

F

S

F

F

S

S

P

H

K

R

A

A

A

V

E

E

A

R

R

Y

A

K

R

R

L

R

P

N

R

T

V

K

S

V

R

K

Y

V

W

Y

D

Y

W

W

E

D

P

-

R

M

A

S

N

L

P

V

E

G

E

D

F

Y

Y

W

Q

G

Y

C

F

F

G

G

G

R

T

P

A

R

P

I

T
x
Y

H

H

-

T

-

I

-

S

-

T

-

L

L

L

Y

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

L

M

I

A

H

C

G

E

E

E

G

G

M

L

E

P

A

A

V

L

W

I

A

A

R

R

H

H

H

E

R

D

L

C

A

A

Q

K

A

R

I

L

W

Y

binding 1-butanol: H45 (≠ E41), S155 (≠ T149), Y257 (≠ T254)

Sites not aligning to the query:

binding 1-butanol: 356

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
25% identity, 82% coverage: 17:363/421 of query aligns to 24:359/383 of 3kgxA

query
sites
3kgxA

P

P

G

G

P

P

S

S

V

N

I

L

P

A

D

P

A

R

V

V

L

L

Q

A

A

A

M

G

H

S

R

L

P

R

S

M

P

I

N

G

I

H

Y

M

A

Q

G

K

E

E

L

M

V

L

E

Q

M

I

T

M

A

E

T

E

L

I

I

K

P

Q

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

V

S

S

-

G

N

S

G

G

H

H

G

C

A

A

W

M

E

E

A

T

A

A

L

L

Q

F

N

N

T

L

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

T

A

G

S

T

S

N

G

G

R

I

F

W

A

G

I

M

G

R

W

A

S

A

E

E

M

I

A

A

E

D

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

Q

L

M

D

I

F

K

G

K

T

P

G

G

A

E

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

L

F

A

L

V

V

H

H

V

G

D

E

T

S

S

S

S

T

S

G

I

V

R

-

N

-

D

-

V

V

A

Q

A

P

M

L

R

D

A

G

A

F

L

G

D

E

A

L

C

C

D

H

-

R

H

Y

P

Q

A

C

L

L

M

L

A

V

D

D

C

S

I

V

A

A

S

S

L

L

G

G

C

G

D

V

R

P

F

I

E

Y

M

M

D

D

A

Q

W

Q

G

G

V

I

D

D

V

I

M

M

V

Y

A

S

C

S

Q

Q

K

K

G

V

L

L

M

N

V

A

P

P

A

P

G

G

M

I

G

S

F

L

V

I

F

S

F

F

S

N

P

D

K

K

A

A

A

-

E

-

A

-

R

-

A

-

R

-

L

-

P

-

R

-

V

-

S

-

R

K

Y

Y

W

K

D

V

W

Y

E

S

P

R

R

K

A

T

N

K

P

P

E

V

E

S

F

F

Y

Y

-

T

-

D

-

I

Q

T

Y

Y

F

L

G

A

G

K

-

L

-

W

-

G

-

C

-

E

-

G

-

V

-

I

-

H

T

T

A

T

P

P

T

V

H

T

H

S

L

L

Y

Y

G

C

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I

I

H

A

G

E

E

Q

G

G

M

L

E

E

A

N

V

C

W

W

A

R

R

R

H

H

H

R

R

E

L

A

A

T

Q

A

A
x
H

I

L

W

H

A

K

A
x
H

C

L

D

Q

R

E

W

M

G

G

D

-

G

-

P

-

L

L

R

K

M

F

N

F

V

V

Q

K

D

D

V

P

A

E

L

I

R

R

S

L

N

P

A

T

V

I

T

T

S

T

L

V

H

T

L

V

-

P

G

A

G

G

D

Y

E

N

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E

L

Q

D

T

H

L

F

G

S

L

I

T

E

L

I

G

S

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

T

W

E

H

E

G

R

F

V

L

L

R

R

L

I

G

G

H

L

M

L

G

G

H

Y

binding magnesium ion: H290 (≠ A285), H294 (≠ A289)

Sites not aligning to the query:

binding magnesium ion: 367

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
27% identity, 82% coverage: 17:362/421 of query aligns to 23:360/384 of 6rv0A

query
sites
6rv0A

P

P

G

G

P

P

S

S

V

N

I

L

P

P

D

P

A

R

V

I

L

M

Q

A

A

A

M

G

H

G

R

L

P

Q

S

M

P

I

N

G

I

S

Y

M

A

S

G

K

E

D

L

M

V

Y

E

Q

M

I

T

M

A

D

T

E

L

I

I

K

P

E

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

I

S

S

-

G

N
x
S

G
|
G

H
|
H

G

C

A

A

W

L

E

E

A

A

L

L

Q

V

N

N

T

V

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

V

A

G

S

A

S

N

G

G

R

I

F
x
W

A

G

I

Q

G

R

W

A

S

V

E

D

M

I

A

G

E

E

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

P

L

M

D

T

F

K

G

D

T

P

G

G

A

G

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

L

F

A

L

V

T

H

H

V

G

D

E

T

S

S

S

S

T

S

G

I

V

R

-

N

-

D

-

V

L

A

Q

A

P

M

L

R

D

A

G

A

F

L

G

D

E

A

L

C

C

D

H

-

R

H

Y

P

K

A

C

L

L

M

L

A

V

D
|
D

C

S

I
x
V

A
|
A

S

S

L

L

G

G

C

G

D

T

R

P

F

L

E

Y

M

M

D

D

A

R

W

Q

G

G

V

I

D

D

V

I

M

L

V

Y

A

S

A

G

C

S

Q

Q

K
|
K

G

A

L

L

M

N

V

A

P

P

A

P

G

G

M

T

G

S

F

L

V

I

F

S

F

F

S

S

P

D

K

K

A

A

A

K

E

K

-

K

-

M

-

Y

A

S

R

R

A

K

R

T

L

K

P

P

R

-

V

F

S

S

R

F

Y

Y

W

L

D

D

W

I

E

K

P

W

R

L

A

A

N

N

-

F

-

W

-

G

P

C

E

D

F

Q

Y

P

Q

R

Y

M

F

Y

G

H

T

T

A

I

P

P

T

V

H

I

H

S

L

L

Y

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I

I

H

A

G

E

E

Q

G

G

M

L

E

E

A

N

V

S

W

W

A

R

R

Q

H

H

H

R

R

E

L

A

A

A

Q

A

A

Y

I

L

W

H

A

G

A

R

C

L

D

Q

R

A

W

L

G

G

D

-

G

-

P

-

L

L

R

Q

M

L

N

F

V

V

Q

K

D

D

V

P

A

A

L

L

R

R

S

L

N

P

A

T

V

V

T

T

S

T

L

V

H

A

L

V

-

P

G

A

G

G

D

Y

E

D

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E

I

Q

D

T

H

L

F

G

D

L

I

T

E

L

I

G

M

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

S

W

T

H

G

G

K

F

V

L

L

R

R

L

I

G

G

H

L

M

L

G

G

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (≠ N69), G77 (= G70), H78 (= H71), W103 (≠ F96), D178 (= D176), V180 (≠ I178), A181 (= A179), K204 (= K202)

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
25% identity, 82% coverage: 17:363/421 of query aligns to 28:364/388 of 3kgwB

query
sites
3kgwB

P

P

G

G

P

P

S

S

V

N

I

L

P

A

D

P

A

R

V

V

L

L

Q

A

A

A

M

G

H

S

R

L

P

R

S

M

P

I

N

G

I

H

Y

M

A

Q

G

K

E

E

L

M

V

L

E

Q

M

I

T

M

A

E

T

E

L

I

I

K

P

Q

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

V

S

S

-

G

N
x
S

G
|
G

H
|
H

G

C

A

A

W

M

E

E

A

T

A

A

L

L

Q

F

N

N

T

L

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

T

A

G

S

T

S

N

G

G

R

I

F
x
W

A

G

I

M

G

R

W

A

S

A

E

E

M

I

A

A

E

D

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

Q

L

M

D

I

F

K

G

K

T

P

G

G

A

E

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

L

F

A

L

V

V

H

H

V

G

D

E

T
x
S

S

S

S

T

S

G

I

V

R

-

N

-

D

-

V

V

A

Q

A

P

M

L

R

D

A

G

A

F

L

G

D

E

A

L

C

C

D

H

-

R

H

Y

P

Q

A

C

L

L

M

L

A

V

D
|
D

C

S

I
x
V

A

A

S

S

L

L

G

G

C

G

D

V

R

P

F

I

E

Y

M

M

D

D

A

Q

W

Q

G

G

V

I

D

D

V

I

M

M

V

Y

A

S

C

S

Q

Q

K
|
K

G

V

L

L

M

N

V

A

P

P

A

P

G

G

M

I

G

S

F

L

V

I

F

S

F

F

S

N

P

D

K

K

A

A

A

-

E

-

A

-

R

-

A

-

R

-

L

-

P

-

R

-

V

-

S

-

R

K

Y

Y

W

K

D

V

W

Y

E

S

P

R

R

K

A

T

N

K

P

P

E

V

E

S

F

F

Y

Y

-

T

-

D

-

I

-

T

-

Y

-

L

-

A

-

K

-

L

-

W

-

G

-

C

-

E

-

T

Q

R

Y

V

F

I

G

H

T

T

A

T

P

P

T

V

H

T

H

S

L

L

Y

Y

G

C

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I

I

H

A

G

E

E

Q

G

G

M

L

E

E

A

N

V

C

W

W

A

R

R

R

H

H

H

R

R

E

L

A

A

T

Q

A

A

H

I

L

W

H

A

K

A

H

C

L

D

Q

R

E

W

M

G

G

D

-

G

-

P

-

L

L

R

K

M

F

N

F

V

V

Q

K

D

D

V

P

A

E

L

I

R

R

S

L

N

P

A

T

V

I

T

T

S

T

L

V

H

T

L

V

-

P

G

A

G

G

D

Y

E

N

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E

L

Q

D

T

H

L

F

G

S

L

I

T

E

L

I

G

S

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

T

W

E

H

E

G

R

F

V

L

L

R

R

L

I

G

G

H

L

M

L

G

G

H

Y

binding pyridoxal-5'-phosphate: S81 (≠ N69), G82 (= G70), H83 (= H71), W108 (≠ F96), S158 (≠ T149), D183 (= D176), V185 (≠ I178), K209 (= K202)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
27% identity, 82% coverage: 17:362/421 of query aligns to 23:360/385 of 5hhyA

query
sites
5hhyA

P

P

G

G

P

P

S

S

V

N

I

L

P

P

D

P

A

R

V

I

L

M

Q

A

A

A

M

G

H

G

R

L

P

Q

S

M

P

I

N

G

I

S

Y

M

A

S

G

K

E

D

L

M

V

Y

E

Q

M

I

T

M

A

D

T

E

L

I

I

K

P

E

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

I

S

S

-

G

N
x
S

G
|
G

H
|
H

G

C

A

A

W

L

E

E

A

A

L

L

Q

V

N

N

T

V

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

V

A

G

S

A

S

N

G

G

R

I

F
x
W

A

G

I

Q

G

R

W

A

S

V

E

D

M

I

A

G

E

E

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

P

L

M

D

T

F

K

G

D

T

P

G

G

A

G

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

L

F

A

L

V

T

H

H

V

G

D

E

T
x
S

S

S

S

T

S

G

I

V

R

-

N

-

D

-

V

L

A

Q

A

P

M

L

R

D

A

G

A

F

L

G

D

E

A

L

C

C

D

H

-

R

H

Y

P

K

A

C

L

L

M

L

A

V

D
|
D

C

S

I
x
V

A

A

S

S

L

L

G

G

C

G

D

T

R

P

F

L

E

Y

M

M

D

D

A

R

W

Q

G

G

V

I

D

D

V

I

M

L

V

Y

A

S

A

G

C

S

Q
|
Q

K
|
K

G

A

L

L

M

N

V

A

P

P

A

P

G

G

M

T

G

S

F

L

V

I

F

S

F

F

S

S

P

D

K

K

A

A

A

K

E

K

-

K

-

M

-

Y

A

S

R

R

A

K

R

T

L

K

P

P

R

-

V

F

S

S

R

F

Y

Y

W

L

D

D

W

I

E

K

P

W

R

L

A

A

N

N

-

F

-

W

-

G

P

C

E

D

F

Q

Y

P

Q

R

Y

M

F
x
Y

G

H

T
|
T

A

I

P

P

T

V

H

I

H

S

L

L

Y

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I

I

H

A

G

E

E

Q

G

G

M

L

E

E

A

N

V

S

W

W

A

R

R

Q

H

H

H

R

R

E

L

A

A

A

Q

A

A

Y

I

L

W

H

A

G

A

R

C

L

D

Q

R

A

W

L

G

G

D

-

G

-

P

-

L

L

R

Q

M

L

N

F

V

V

Q

K

D

D

V

P

A

A

L

L

R

R

S

L

N

P

A

T

V

V

T

T

S

T

L

V

H

A

L

V

-

P

G

A

G

G

D

Y

E

D

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E

I

Q

D

T

H

L

F

G

D

L

I

T

E

L

I

G

M

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

S

W

T

H

G

G

K

F

V

L

L

R

R

L

I

G

G

H

L

M

L

G

G

binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ N69), G77 (= G70), H78 (= H71), W103 (≠ F96), S153 (≠ T149), D178 (= D176), V180 (≠ I178), Q203 (= Q201), K204 (= K202), Y255 (≠ F248), T258 (= T251)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
27% identity, 82% coverage: 17:362/421 of query aligns to 28:365/392 of P21549

query
sites
P21549

P

P

G

G

P

P

S

S

V

N

I

L

P

P

D

P

A
x
R

V

I

L

M

Q

A

A

A

M
x
G

H

G

R

L

P

Q

S

M

P

I

N
x
G

I

S

Y

M

A

S

G

K

E

D

L

M

V

Y

E

Q

M

I

T

M

A

D

T

E

L

I

I

K

P

E

D

G

L

I

R

Q

R

Y

V

V

A

F

R

Q

T

T

E

R

H

N

N

P

V

L

A

T

I

L

Y

V

I

I

S

S

-

G

N

S

G
|
G

H

H

G

C

A

A

W

L

E

E

A

A

L

L

Q

V

N

N

T

V

L

L

Q

E

P

P

G

G

D

D

T

S

V

F

L

L

V

V

A

G

S

A

S

N

G

G

R

I

F
x
W

A

G

I

Q

G

R

W
x
A

S

V

E

D

M

I

A

G

E

E

A

R

L

I

G

G

I

A

K

R

V

V

E

H

L

P

L

M

D

T

F

K

G

D

T

P

G

G

A

G

P

H

W

Y

D

T

M

L

D

Q

R

E

I

V

A

E

S

E

H

G

L

L

A

A

A

Q

D

-

T

-

A

-

H

H

R

K

I

P

K

V

A
x
L

V

L

L
x
F

A

L

V

T

H

H

V
x
G

D

E

T
x
S

S

S

S

T

S
x
G

I

V

R

-

N

-

D

-

V

L

A

Q

A

P

M
x
L

R

D

A

G

A

F

L
x
G

D

E

A

L

C
|
C

D

H

-

R

H

Y

P

K

A

C

L

L

M

L

A

V

D
|
D

C

S

I

V

A

A

S
|
S

L

L

G

G

C

G

D

T

R

P

F

L

E

Y

M

M

D

D

A

R

W

Q

G

G

V

I

D

D

V
x
I

M

L

V

Y

A
x
S

A

G

C

S

Q

Q

K
|
K

G

A

L

L

M

N

V

A

P

P

A

P

G

G

M

T

G
x
S

F

L

V

I

F

S

F

F

S

S

P

D

K

K

A

A

A

K

E

K

-

K

-

M

-

Y

A

S

R
|
R

A

K

R

T

L

K

P

P

R

-

V

F

S

S

R

F

Y

Y

W

L

D

D

W
x
I

E

K

P

W

R

L

A

A

N

N

-

F

-

W

-

G

P
x
C

E

D

F

Q

Y

P

Q

R

Y

M

F

Y

G

H

T

T

A

I

P

P

T

V

H

I

H

S

L

L

Y

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

L

L

I
|
I

H
x
A

G

E

E

Q

G

G

M

L

E

E

A

N

V

S

W

W

A

R

R

Q

H

H

H

R

R

E

L

A

A

A

Q

A

A

Y

I

L

W

H

A

G

A

R

C

L

D

Q

R

A

W

L

G

G

D

-

G

-

P

-

L

L

R

Q

M

L

N

F

V

V

Q

K

D

D

V

P

A

A

L

L

R

R

S

L

N

P

A

T

V

V

T

T

S

T

L

V

H

A

L
x
V

-

P

G

A

G

G

D

Y

E

D

A

W

T

R

R

D

L

I

R

V

T

S

W

Y

V

V

E
x
I

Q

D

T

H

L

F

G

D

L

I

T

E

L

I

G

M

I

G

G

G

L

L

G

G

M

-

A

-

P

-

N

-

S

-

P

P

E

S

W

T

H

G

G

K

F

V

L

L

R

R

L

I

G

G

H

L

M

L

G

G

R36 (≠ A25) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M30) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (≠ N36) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G70) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F96) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W100) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ A141) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ L143) to I: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein destabilization; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; mitochondrial mistargeting; dbSNP:rs121908524
G156 (≠ V147) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T149) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (≠ S152) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ M160) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ L164) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in mitochondrial mistargeting; slight decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; dbSNP:rs121908529
C173 (= C167) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D176) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S180) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ V195) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (≠ A198) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K202) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ G211) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (= R223) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ W235) to T: in HP1; prevalent mutation in the Canary islands; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; partial mitochondrial mistargeting; dbSNP:rs121908525
C253 (≠ P241) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (= I267) to T: in dbSNP:rs140992177
A280 (≠ H268) to V: in dbSNP:rs73106685
V326 (≠ L318) to I: in dbSNP:rs115057148
I340 (≠ E331) to M: associated with hyperoxaluria; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: reduction of specific alanine--glyoxylate aminotransferase activity in vitro; causes mitochondrial mistargeting when associated with R-170; dbSNP:rs34116584

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
30% identity, 66% coverage: 17:294/421 of query aligns to 24:299/377 of 1vjoA

query
sites
1vjoA

P

P

G

G

P

P

S

S

V

N

I

A

P

H

D

P

A

S

V

V

L

L

Q

Q

A

A

M

M

H

N

R

V

P

S

S

P

P

V

N

G

I

H

Y

L

A

D

G

P

E

A

L

F

V

L

E

A

M

L

T

M

A

D

T

E

L

I

I

Q

P

S

D

L

L

L

R

R

R

Y

V

V

A

W

R

Q

T

T

E

E

H

N

N

P

V

L

A

T

I

I

Y

A

I

V

S

S

-

G

N
x
T

G
|
G

H
x
T

G

A

A

A

W

M

E

E

A

A

A

T

L

I

Q

A

N

N

T

A

L

V

Q

E

P

P

G

G

D

D

T

V

V

V

L

L

V

I

A

G

S

V

S

A

G

G

R

Y

F
|
F

A

G

I

N

G

R

W

L

S

V

E

D

M

M

A

A

E

G

A

R

L

Y

G

G

I

A

K

D

V

V

E

R

L

T

L

I

D

S

F

K

G

P

T

W

G

G

A

E

P

V

W

F

D

S

M

L

D

E

R

E

I

L

A

R

S

T

H

A

L

L

A

E

A

T

D

-

T

-

A

-

H

H

R

R

I

P

K

A

A

I

V

L

L

A

A

L

V

V

H

H

V

A

D

E

T
|
T

S

S

S

T

S

G

I

A

R

R

N

Q

D

P

V

L

A

E

A

G

M

V

R

G

A

-

L

-

D

E

A

L

C

C

-

R

D

E

H

F

P

G

A

T

L

L

M

L

A

V

D
|
D

C

T

I
x
V

A

T

S

S

L

L

G

G

C

G

D

V

R

P

F

I

E

F

M

L

D

D

A

A

W

W

G

G

V

V

D

D

V

L

M

A

V

Y

A

S

A

C

C

S

Q

Q

K
|
K

G

G

L

L

M

G

V

C

P

S

A

P

G

G

M

A

G

S

F

P

V

F

F

T

F

M

S

S

P

S

K

R

A

A

A

I

E

E

A

K

R

L

A

Q

R

R

L

-

P

-

R

R

V

R

S

T

R

K

Y

V

W

A

D

N

W

W

E

Y

P

L

R

D

A

M

N

N

P

L

E

L

E

G

F

K

Y

Y

-

W

-

G

-

S

-

E

Q

R

Y

V

F

Y

G

H

T

T

A

A

P

P

T

I

H

N

H

L

L

Y

Y

Y

G

A

L

L

R

R

A

E

A

A

L

L

D

R

L

L

I

I

H

A

G

Q

E

E

G

G

M

L

E

A

A

N

V

C

W

W

A

Q

R

R

H

H

H

Q

R

K

L

N

A

V

Q

E

A

Y

I

L

W

W

A

E

A

R

C

L

D

E

R

D

W

I

G

G

binding pyridoxal-5'-phosphate: T77 (≠ N69), G78 (= G70), T79 (≠ H71), F104 (= F96), T154 (= T149), D179 (= D176), V181 (≠ I178), K205 (= K202)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
27% identity, 91% coverage: 14:398/421 of query aligns to 10:384/387 of 3islA

query
sites
3islA

L

I

A

M

I

T

P

P

G

G

P

P

S

V

V

E

I

V

P

D

D

P

A

R

V

V

L

L

Q

R

A

V

M

M

H

S

R

T

P

P

S

V

P

V

N

G

I

Q

Y

F

A

D

G

P

E

A

L

F

V

T

E

G

M

I

T

M

A

N

T

E

L

T

I

M

P

E

D

M

L

L

R

R

R

E

V

L

A

F

R

Q

T

T

E

K

H

N

N

R

V

W

A

A

I

Y

Y

P

I

I

S

D

-

G

N
x
T

G
x
S

H
x
R

G

A

A

G

W

I

E

E

A

A

A

V

L

L

Q

A

N

S

T

V

L

I

Q

E

P

P

G

E

D

D

T

D

V

V

L

L

V

I

A

P

S

I

S

Y

G

G

R

R

F
|
F

A

G

I

Y

G

L

W

L

S

T

E

E

M

I

A

A

E

E

A

R

L

Y

G

G

I

A

K

N

V

V

E

H

L

M

L

L

D

E

F

C

G

E

T

W

G

G

A

T

P

V

W

F

D

D

M

P

D

E

R

D

I

I

A

I

S

R

H

E

L

I

A

K

D

-

T

-

A

-

H

V

R

K

I

P

K

K

A

I

V

V

L

A

A

M

V

V

H

H

V

G

D

E

T
|
T

S

S

S

T

S

G

I

-

R

-

N

-

D

R

V

I

A

H

A

P

M

L

R

K

A

A

A

I

L

G

D

E

A

A

C

C

-

R

D

T

H

E

P

D

A

A

L

L

L

F

M

I

A

V

D
|
D

C

A

I
x
V

A

A

S

T

L

I

G

G

C

G

D

C

R

E

F

V

E

K

M

V

D

D

A

E

W

W

G

K

V

I

D

D

V

A

M

A

V

I

A

G

C

T

Q
|
Q

K
|
K

G

C

L

L

M

S

V

V

P

P

A

S

G

G

M

M

G

A

F

P

V

I

F

T

F

Y

S

N

P

E

K

R

A

V

A

A

E

D

-

V

-

I

-

A

A

A

R

R

A

N

R

R

L

-

P

P

R

I

V

T

S

S

R

N

Y

Y

W

F

D

D

W

L

E

S

P

Q

R

L

A

E

N

D

P

Y

E

W

E

S

F

E

Y

R

Q

R

Y

L

F

N

G

H

T

T

A

E

P

A

T

T

H

T

H

M

L

L

Y

Y

G

A

L

L

R

R

A

E

A

G

L

V

D

R

L

L

I

V

H

L

G

E

E

E

G

G

M

L

E

E

A

T

V

R

W

F

A

E

R

R

H

H

H

R

R

H

L

H

A

E

Q

A

A

A

I

L

W

A

A

A

A

G

C

I

D

K

R

A

W

M

G

G

D

-

G

-

P

-

L

L

R

R

M

L

N

-

V

F

Q

G

D

D

V

D

A

S

L

C

R

K

S

M

N

P

A

V

V

V

T

T

S

C

L

V

H

E

L

I

-

P

G

G

D

I

E

D

A

G

T

E

R

S

L

V

R

R

T

D

W

M

V

L

E

L

Q

A

T

Q

L

F

G

G

L

I

T

E

L

I

G

A

I

S

G

S

L

F

G

G

M

-

A

-

P

P

P

L

N

A

S

G

P

K

E

I

W

W

H

-

G

-

F

-

L

-

R

R

L

I

G

G

H

T

M

M

G

G

H

Y

-

S

V

C

S

R

G

K

Q

E

M

N

I

V

M

L

G

F

L

V

L

L

G

-

V

-

D

-

A

A

G

G

L

L

K

E

A

A

L

V

E

L

I

L

P

R

H

H

G

N

S

A

G

G

-

I

-

E

-

A

-

G

-

K

A

A

L

L

E

Q

A

A

S

L

Q

D

V

V

binding pyridoxal-5'-phosphate: T66 (≠ N69), S67 (≠ G70), R68 (≠ H71), F93 (= F96), T143 (= T149), D168 (= D176), V170 (≠ I178), Q193 (= Q201), K194 (= K202)

Query Sequence

>GFF2363 FitnessBrowser__Phaeo:GFF2363
MTADVSLAAGRGYLAIPGPSVIPDAVLQAMHRPSPNIYAGELVEMTATLIPDLRRVARTE
HNVAIYISNGHGAWEAALQNTLQPGDTVLVASSGRFAIGWSEMAEALGIKVELLDFGTGA
PWDMDRIASHLAADTAHRIKAVLAVHVDTSSSIRNDVAAMRAALDACDHPALLMADCIAS
LGCDRFEMDAWGVDVMVAACQKGLMVPAGMGFVFFSPKAAEARARLPRVSRYWDWEPRAN
PEEFYQYFGGTAPTHHLYGLRAALDLIHGEGMEAVWARHHRLAQAIWAACDRWGDGGPLR
MNVQDVALRSNAVTSLHLGGDEATRLRTWVEQTLGLTLGIGLGMAPPNSPEWHGFLRLGH
MGHVSGQMIMGLLGGVDAGLKALEIPHGSGALEAASQVIAGAGATAVPASPELSAVGSCC
G

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory