SitesBLAST

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 84% coverage: 19:127/129 of query aligns to 16:128/128 of Q7CP78

query
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Q7CP78

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Y17 (≠ F20) mutation to F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
R105 (= R105) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E119) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
37% identity, 84% coverage: 19:126/129 of query aligns to 20:129/137 of P80601

query
sites
P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 82% coverage: 19:124/129 of query aligns to 78:185/187 of Q94JQ4

query
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Q94JQ4

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R165 (= R105) mutation to A: Loss of activity.

3vczB 1.80 angstrom resolution crystal structure of a putative translation initiation inhibitor from vibrio vulnificus cmcp6
33% identity, 84% coverage: 19:126/129 of query aligns to 15:127/127 of 3vczB

query
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3vczB

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binding magnesium ion: D79 (= D82), N81 (≠ R84)

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
34% identity, 83% coverage: 20:126/129 of query aligns to 21:129/137 of P52759

query
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P52759

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
33% identity, 84% coverage: 19:126/129 of query aligns to 15:126/127 of 2uynA

query
sites
2uynA

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binding 2-ketobutyric acid: F83 (= F86), R104 (= R105), S105 (≠ A106), C106 (= C107)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
33% identity, 84% coverage: 19:126/129 of query aligns to 15:126/127 of 2uykC

query
sites
2uykC

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binding serine: R104 (= R105), S105 (≠ A106), C106 (= C107)

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
30% identity, 81% coverage: 21:124/129 of query aligns to 1:106/108 of 5hp8A

query
sites
5hp8A

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binding pyruvic acid: R86 (= R105), T88 (≠ C107)

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
32% identity, 88% coverage: 10:123/129 of query aligns to 8:124/128 of A0A1J1DL12

query
sites
A0A1J1DL12

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Y19 (≠ F20) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G34) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (= N58) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N90) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R105) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (≠ M113) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E119) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
29% identity, 82% coverage: 19:124/129 of query aligns to 17:125/127 of 5v4dA

query
sites
5v4dA

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V

I

V

V

R

K

V

T

G

T

V

I

W

F

L

I

E

T

D

N

P

I

R

N

D

N

F

S

W

S

S

Q

F

V

N

N

K
x
D

V

I

F

Y

G

A

E
x
D

Y

Y

F

F

T

K

P

G

E

T

H

I

A

F

P

P

A

A

R

R

A

S

C

T

V

V

Q

E

A

V

N

S

M

A

M

L

V

P

D

K

-

G

C

A

K

L

V

V

E

E

I

I

D

E

C

V

V

I

A

A

binding calcium ion: D91 (≠ K91), D95 (≠ E95)

3k0tC Crystal structure of pspto -psp protein in complex with d-beta-glucose from pseudomonas syringae pv. Tomato str. Dc3000 (see paper)
31% identity, 81% coverage: 19:123/129 of query aligns to 15:120/124 of 3k0tC

query
sites
3k0tC

P

P

F
x
Y

A

S

R

Q

A

A

V

I

E

K

A

A

D

G

G

N

W

T

L

V

Y

Y

V

M

S

S

G
|
G

Q

Q

V
x
I

P

P

A

L

V

D

D

P

G

S

E

T

I

M

-

E

I

L

T

V

G

E

G

G

I

I

I

E

E

A

Q

Q

T

I

R

T

Q

Q

T

V

M

F

R

E

N

N

V

L

V

K

A

S

I

V

L

A

E

Q

E

A

A

A

G

G

Y

G

E

S

L

F

K

K

D

D

V

I

V

V

R

K

V

L

G

N

V

I

W

F

L

L

E

T

D

D

P

L

R

G

D

H

F
|
F

W

A

S

K

F

V

N

N

K

E

V

I

F

M

G

G

E

S

Y

Y

F

F

T

S

P

Q

E

P

H

Y

A

-

P

P

A

A

R
|
R

A

A

C

A

V

I

-

G

Q

V

A

A

N

A

M

L

M

P

V
x
R

D

G

C

A

K

Q

V

V

E
|
E

I

M

D

D

C

A

V

I

binding beta-D-glucopyranose: Y16 (≠ F20), G30 (= G34), I32 (≠ V36), F83 (= F86), R101 (= R105), R111 (≠ V114), E116 (= E119)

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
31% identity, 91% coverage: 12:128/129 of query aligns to 9:128/128 of P0AFQ5

query
sites
P0AFQ5

A

A

G

G

G

S

Q

A

P

P

R

L

-

A

P

P

F

F

A

V

R

P

A

G

V

T

E

L

A

A

D

D

G

G

W

V

L

V

Y

Y

V

V

S

S

G

G

Q

T

V

L

P

-

A

A

V

F

D

D

G

Q

E

H

-

N

-

V

I

L

I

F

T

A

G

D

I

P

I

K

E

A

Q

Q

T

T

R

R

Q

H

T

V

M

L

R

E

N

T

V

I

V

R

A

K

I

V

L

I

E

E

E

T

A

A

G

G

Y

G

E

T

L

M

K

A

D

D

V

V

V

T

R

F

V

N

G

S

V

I

W

F

L

I

E

T

D

D

P

W

R

K

D

N

F

Y

W

A

S

A

F

I

N

N

K

E

V

I

F

Y

G

A

E

E

Y

F

F

F

T

-

P

P

E

G

H

D

A

K

P

P

A

A

R
|
R

A

F

C

C

V

I

Q

Q

A

C

N

G

M

L

M

V

-

K

V

P

D

D

C

A

K

L

V

V

E

E

I

I

D

A

C

T

V

I

A

A

Y

H

K

I

K

A

K

K

R104 (= R105) mutation to A: Loss of activity.

3i3fA Hypothetical protein from giardia lamblia gl50803_14299
30% identity, 79% coverage: 27:128/129 of query aligns to 26:131/131 of 3i3fA

query
sites
3i3fA

D

N

G

G

W

M

L

V

Y

Y

V

L

S

G

G
|
G

Q

S

V

V

P

G

A

I

V

D

D

K

G

S

E

G

I

T

I

L

T

H

G

K

G

G

I

L

I

E

E

E

Q

Q

T

T

R

R

Q

Q

T

T

M

F

R

D

N

N

V

I

V

R

A

K

I

C

L

L

E

E

E

Y

A

A

G

N

Y

S

E

G

L

L

K

D

D

Y

V

I

V

V

R

S

V

L

G

N

V

I

W

F

L

L

E

S

D

T

P

S

-

L

-

S

-

D

R

S

D

E

F

E

W

A

S

R

F

F

N

N

K

E

V

L

F

Y

G

R

E

E

Y

V

F

F

-

C

T

V

P

P

E

A

H

T

A

R

P

P

A

C

R
|
R

A
x
C

C
|
C

V

V

Q

R

A

A

N

Q

M

L

M

Q

V

E

D

G

C

L

K

L

V

V

E

E

I

V

D

V

C

N

V

V

A

V

Y

A

K

A

K

Q

K

K

binding pentanoic acid: G33 (= G34), R108 (= R105), C109 (≠ A106), C110 (= C107)

Sites not aligning to the query:

binding pentanoic acid: 16, 19

3i3fB Hypothetical protein from giardia lamblia gl50803_14299
30% identity, 79% coverage: 27:128/129 of query aligns to 23:128/128 of 3i3fB

query
sites
3i3fB

D

N

G

G

W

M

L

V

Y

Y

V

L

S

G

G

G

Q

S

V

V

P

G

A

I

V

D

D

K

G

S

E

G

I

T

I

L

T

H

G

K

G

G

I

L

I

E

E

E

Q

Q

T

T

R

R

Q

Q

T

T

M

F

R

D

N

N

V

I

V

R

A

K

I

C

L

L

E

E

E

Y

A

A

G

N

Y

S

E

G

L

L

K

D

D

Y

V

I

V

V

R

S

V

L

G

N

V

I

W

F

L

L

E

S

D

T

P

S

-

L

-

S

-

D

R

S

D

E

F

E

W

A

S

R

F

F

N

N

K

E

V

L

F

Y

G

R

E

E

Y

V

F

F

-

C

T

V

P

P

E

A

H

T

A

R

P

P

A

C

R
|
R

A
x
C

C
|
C

V

V

Q

R

A

A

N

Q

M

L

M
x
Q

V

E

D

G

C

L

K

L

V

V

E

E

I

V

D

V

C

N

V

V

A

V

Y

A

K

A

K

Q

K

K

binding butanoic acid: R105 (= R105), C106 (≠ A106), C107 (= C107)
binding pentanoic acid: Q113 (≠ M113)

Sites not aligning to the query:

binding pentanoic acid: 13, 16

Query Sequence

>GFF2376 FitnessBrowser__WCS417:GFF2376
MSITRYGAGSTAGGGQPRPFARAVEADGWLYVSGQVPAVDGEIITGGIIEQTRQTMRNVV
AILEEAGYELKDVVRVGVWLEDPRDFWSFNKVFGEYFTPEHAPARACVQANMMVDCKVEI
DCVAYKKKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory