SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2521 FitnessBrowser__WCS417:GFF2521 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3nvsA 1.02 angstrom resolution crystal structure of 3-phosphoshikimate 1- carboxyvinyltransferase from vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate
37% identity, 98% coverage: 5:412/418 of query aligns to 2:424/426 of 3nvsA

query
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3nvsA

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active site: K22 (= K26), S23 (= S27), D49 (= D53), N94 (= N95), P119 (≠ D120), R124 (= R125), H128 (≠ P129), Q135 (= Q136), Y142 (vs. gap), E144 (≠ S143), A247 (= A246), A255 (≠ V254), D314 (= D297), E342 (= E325), H386 (= H374), R387 (= R375), K412 (= K400)
binding glyphosate: K22 (= K26), N94 (= N95), A95 (= A96), G96 (= G97), T97 (= T98), R124 (= R125), Q172 (= Q172), D314 (= D297), E342 (= E325), R345 (= R328), H386 (= H374), R387 (= R375), K412 (= K400)
binding magnesium ion: E123 (≠ K124), Q145 (≠ P144)
binding shikimate-3-phosphate: K22 (= K26), S23 (= S27), R27 (= R31), T97 (= T98), S170 (= S170), S171 (= S171), Q172 (= Q172), S198 (≠ A199), Y201 (= Y202), D314 (= D297), N337 (= N320), K341 (= K324)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: K22 (= K26), S23 (= S27), R27 (= R31), T97 (= T98), Q172 (= Q172), Y201 (= Y202), D314 (= D297), K341 (= K324)

Q9KRB0 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
37% identity, 98% coverage: 5:412/418 of query aligns to 2:424/426 of Q9KRB0

query
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Q9KRB0

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KS 22:23 (= KS 26:27) binding
R27 (= R31) binding
NAGT 94:97 (= NAGT 95:98) Phosphoenolpyruvate
R124 (= R125) binding
SSQ 170:172 (= SSQ 170:172) binding
S198 (≠ A199) binding
N337 (= N320) binding
K341 (= K324) binding
R345 (= R328) binding
R387 (= R375) binding

7tm6A Crystal structure of shikimate-3-phosphate and glyphosate bound 3- phosphoshikimate 1-carboxyvinyltransferase from klebsiella pneumoniae
36% identity, 95% coverage: 16:412/418 of query aligns to 11:422/426 of 7tm6A

query
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7tm6A

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K

I

V

R

-

P

-

G

G

L

A

A

E

T

V

I

E

E

E

G

G

D

E

D

D

L

Y

L

I

V

R

A

I

S

T

D

P

P

P

A

A

L

K

A

L

G

K

T

Y

S

-

C

-

N

-

A

A

L

E

I

I

D

G

T

T

H

Y

A

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

C

C

F

F

A

S

L

L

A

V

G

A

L

L

K

S

V

D

A

T

G

P

I

V

R

T

I

I

Q

L

D

D

P

P

D

K

C

C

V

T

A

A

K
|
K

T

T

Y

F

P

P

E

D

Y

Y

W

F

K

E

A

Q

L

L

G

A

S

R

L

I

binding glyphosate: K21 (= K26), N93 (= N95), A94 (= A96), G95 (= G97), T96 (= T98), R123 (= R125), Q170 (= Q172), D312 (= D297), E340 (= E325), R343 (= R328), H384 (= H374), R385 (= R375), K410 (= K400)
binding shikimate-3-phosphate: K21 (= K26), S22 (= S27), R26 (= R31), T96 (= T98), S168 (= S170), S169 (= S171), Q170 (= Q172), S196 (≠ A199), Y199 (= Y202), D312 (= D297), N335 (= N320), K339 (= K324)

7tm5B Crystal structure of shikimate-3-phosphate bound 3-phosphoshikimate 1- carboxyvinyltransferase from klebsiella pneumoniae
36% identity, 95% coverage: 16:412/418 of query aligns to 12:423/427 of 7tm5B

query
sites
7tm5B

L

V

N

E

G

G

K

T

V

V

A

N

P

L

P

P

G

G

S

S

K
|
K

S
|
S

I

V

T

S

N

N

R
|
R

A

A

L

L

A

A

L

L

A

A

N

R

G

G

T

T

S

T

R

V

L

L

S

T

G

N

A

L

L

L

K

D

S

S

D

D

T

V

R

R

H

H

M

M

S

L

V

N

A

A

L

L

R

S

Q

A

M

L

G

G

V

V

T

Q

I

Y

D

T

-

L

E

S

P

A

D

D

D

R

T

T

T

R

F

C

V

E

V

V

T

T

G

G

Q

N

G

G

K

G

-

P

-

L

L

R

Q

S

L

A

P

A

S

A

Q

L

P

E

L

L

F

F

L

L

G

G

N

N

A

A

G

G

T
|
T

A

A

M

M

R

R

F

P

L

L

T

A

A

A

V

L

A

C

T

L

V

G

E

S

G

N

T

D

V

I

V

V

L

L

D

T

G

G

D

E

D

P

Y

R

M

M

Q

K

K

E

R

R

P

P

I

I

G

G

P

H

L

L

L

V

A

D

T

A

L

L

G

R

Q

Q

N

G

G

G

I

A

L

Q

V

I

D

D

-

C

-

L

S

E

P

Q

T

E

G

N

C

Y

P

P

V

L

T

R

V

L

H

R

G

-

V

-

G

G

K

G

I

F

K

Q

A

G

K

G

R

N

F

V

E

E

I

V

D

D

G

G

G

S

L

V

S
|
S

Q
|
Q

Y

F

V

L

S

T

A

A

L

L

M

M

L

T

A

A

A

P

C

L

G

A

E

P

A

Q

P

D

I

T

E

V

V

I

A

V

L

I

T

K

G

G

K

-

D

D

I

L

G

V

A
x
S

R

K

G

P

Y
|
Y

V

I

D

D

L

I

T

T

L

L

D

H

C

L

M

M

R

K

A

T

F

F

G

G

A

V

Q

E

V

V

E

D

A

-

V

-

D

N

D

Q

T

S

T

Y

W

Q

R

R

V

F

A

V

P

V

T

R

G

G

Y

K

T

Q

A

Q

H

Y

-

Q

-

S

-

P

-

G

D

D

Y

Y

L

L

I

V

E

E

P

G

D

D

A

A

S

S

A

S

A

A

T

S

Y

Y

L

F

W

L

A

A

E

G

V

A

L

I

T

K

G

G

R

T

I

V

D

K

I

V

G

T

V

G

A

I

A

G

Q

R

D

N

F

S

T

V

Q

Q

P

G

D

D

A

I

K

R

A

F

Q

A

A

D

V

V

I

L

A

E

Q

K

F

-

P

-

N

-

M

M

Q

G

A

A

T

T

V

V

V

T

G

W

-

G

-

D

-

F

-

I

-

A

-

C

-

T

-

H

-

G

-

E

-

L

-

K

-

A

-

V

-

D

-

M

-

D

-

M

S

N

Q

H

M

I

Q

P

D
|
D

A

A

I

A

P

M

T

T

L

I

A

A

V

T

L

A

A

A

A

L

F

F

N

A

N

Q

T

G

P

T

V

T

R

T

F

L

T

R

E

N

L

I

A

Y

N
|
N

L

W

R

R

V

V

K
|
K

E

E

C

T

D

D

R

R

V

L

Q

F

A

A

L

M

H

A

D

T

G

E

L

L

N

R

E

K

I

V

R

-

P

-

G

G

L

A

A

E

T

V

I

E

E

E

G

G

D

E

D

D

L

Y

L

I

V

R

A

I

S

T

D

P

P

P

A

A

L

K

A

L

G

K

T

Y

S

-

C

-

N

-

A

A

L

E

I

I

D

G

T

T

H

Y

A

N

D

D

H

H

R

R

I

M

A

A

M

M

C

C

F

F

A

S

L

L

A

V

G

A

L

L

K

S

V

D

A

T

G

P

I

V

R

T

I

I

Q

L

D

D

P

P

D

K

C

C

V

T

A

A

K

K

T

T

Y

F

P

P

E

D

Y

Y

W

F

K

E

A

Q

L

L

G

A

S

R

L

I

binding shikimate-3-phosphate: K22 (= K26), S23 (= S27), R27 (= R31), T97 (= T98), S169 (= S170), S170 (= S171), Q171 (= Q172), S197 (≠ A199), Y200 (= Y202), D313 (= D297), N336 (= N320), K340 (= K324)

7tbuA Crystal structure of the 5-enolpyruvate-shikimate-3-phosphate synthase (epsps) domain of aro1 from candida albicans in complex with shikimate-3-phosphate (see paper)
33% identity, 95% coverage: 13:411/418 of query aligns to 11:443/450 of 7tbuA

query
sites
7tbuA

N

N

F

P

P

P

L

K

N

E

G

N

K

I

V

I

A

V

P

P

G

G

S

S

K

K

S
|
S

I

I

T

S

N

N

R
|
R

A

A

L

L

L

I

L

L

A

A

L

L

A

G

N

N

G

G

T

T

S

V

R

R

L

V

S

K

G

N

A

L

L

L

K

H

S

S

D

D

T

T

R

K

H

H

M

M

S

L

V

D

A

A

L

V

R

A

Q

S

M

L

-

K

G

G

V

A

T

E

I

I

D

S

E

T

P

E

D

D

D

N

-

G

T

E

T

T

F

I

V

V

T

K

G

G

Q

N

-

G

G

G

K

N

L

L

Q

V

L

T

P

S

S

G

Q

E

P

E

L

L

F

Y

L

L

G

G

N

N

A

A

G

G

T

T

A

A

M

S

R

R

F

F

L

L

T

T

A

T

A

V

V

A

A

S

T

L

V

V

E

G

G

K

T

S

-

Q

-

A

-

S

-

D

V

V

V

I

L

L

D

T

G

G

D

N

D

A

Y

R

M

M

Q

Q

K

E

R

R

P

P

I

I

G

G

P

P

L

L

L

V

A

D

T

A

L

L

G

R

Q

S

N

N

G

G

I

S

L

E

V

I

D

E

-

Y

-

L

S

N

P

K

T

Q

G

G

C

S

P

L

P

P

V

L

T

K

V

I

H

S

G

A

V

G

G

N

K

G

I

L

K

K

A

G

K

G

R

R

F

I

E

E

I

L

D

A

G

A

G

T

L

I

S
|
S

Q
|
Q

Y

Y

V

V

S

S

A

S

L

I

L

L

M

M

L

C

A

A

A

P

C

Y

G

A

E

K

A

E

P

P

I

V

E

T

V

L

A

A

L

L

T

V

G

G

K

G

D

K

I

P

G

I

A
x
S

R

Q

G

L

Y
|
Y

V

I

D

D

L

M

T

T

L

C

D

A

C

M

M

M

R

K

A

S

F

F

G

G

A

I

Q

E

V

V

-

T

-

K

E

S

A

T

V

T

D

E

T

Y

T

T

W

Y

R

H

V

I

A

P

P

K

T

G

G

T

Y

Y

-

K

T

N

A

P

H

S

D

E

Y

Y

L

V

I

I

E

E

P

S

D

D

A

A

S

S

A

S

A

A

T

T

Y

Y

L

P

W

L

A

A

A

F

E

A

V

A

L

M

T

T

G

G

G

T

R

S

I

C

D

T

I

I

G

P

V

N

A

I

A

G

Q

S

D

S

F

S

T

L

Q

Q

P

G

D

D

A

A

K

K

-

F

-

A

-

V

-

D

-

V

-

L

-

K

-

P

-

M

-

G

-

C

-

K

-

V

-

E

-

Q

-

T

-

S

-

T

-

V

-

T

-

G

-

P

A

P

Q

R

A

G

V

H

I

L

A

K

Q

P

F

L

P

P

N

H

M

V

Q

D

A

M

T

-

V

-

G

-

S

E

Q

P

M

M

Q

T

D

D

A

A

I

F

P

L

T

T

L

A

A

S

V

V

L

V

A

A

F

V

-

A

-

K

N

G

T

S

P

S

V

T

R

S

F

I

T

T

E

G

L

I

A

A

N

N

L

Q

R

R

V

V

K

K

E

E

C

C

D

N

R

R

V

I

Q

E

A

A

L

M

H

V

D

T

G

E

L

L

-

A

-

K

-

F

-

G

-

V

-

P

-

A

N

N

E

E

I

L

R

P

P

D

G

G

L

I

A

E

T

-

I

I

E

H

G

G

D

I

D

D

L

I

L

E

V

D

A

L

S

K

D

T

P

P

A

E

L

I

A

S

G

K

T

R

S

G

C

-

N

-

A

-

L

-

I

V

D

S

T

S

H

Y

A

D

D

D

H

H

R

R

I

V

A

A

M

M

C

S

F

F

A

S

L

L

A

-

G

-

L

-

K

-

V

L

A

A

G

G

I

L

R

C

I

K

Q

E

D

P

P

V

D

L

C

I

V

L

A

E

K

R

T

T

Y

W

P

P

E

G

Y

W

W

W

K

D

A

I

L

L

G

H

S

S

binding shikimate-3-phosphate: S25 (= S27), R29 (= R31), S178 (= S170), S179 (= S171), Q180 (= Q172), S207 (≠ A199), Y210 (= Y202)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
36% identity, 98% coverage: 6:416/418 of query aligns to 387:832/1555 of 6hqvA

query
sites
6hqvA

T

S

V

V

T

R

V

V

T

T

P

P

P

-

N

G

F

V

P

P

-

K

-

G

L

L

N

S

G

V

K

T

V

V

A

T

P

P

G

G

S

S

K

K

S
|
S

I

I

T

S

N

N

R
|
R

A

A

L

L

L

V

L

L

A

A

L

L

A

G

N

E

G

G

T

T

S

T

R

R

L

I

S

H

G

G

A

L

L

L

K

H

S

S

D

D

T

V

R

Q

H

Y

M

M

S

L

V

A

A

A

L

I

R

E

Q

Q

M

L

-

H

G

G

V

A

T

D

I

F

D

S

E

W

P

E

D

D

-

A

D

G

T

E

T

I

F

L

V

V

T

T

G

G

Q

K

-

G

G

G

K

K

L

L

Q

Q

L

A

P

S

S

K

Q

E

P

P

L

L

F

Y

L

L

G

G

N

N

A

A

G

G

T
|
T

A

A

M

S

R

R

F

F

L

L

T

T

A

S

A

V

V

V

A

A

-

L

-

C

-

A

-

P

T

S

V

A

E

V

G

S

T

S

V

T

V

V

L

L

D

T

G

G

D

N

D

A

Y

R

M

M

Q

K

K

V

R

R

P

P

I

I

G

G

P

A

L

L

L

V

A

D

T

A

L

L

G

R

Q

A

N

N

G

G

I

V

L

G

V

V

D

K

-

Y

-

L

S

E

P

K

T

E

G

K

C

S

P

L

P

P

V

V

T

E

V

V

H

D

G

A

V

A

G

G

K

G

I

F

K

A

A

G

K

G

R

V

F

I

E

E

I

L

D

A

G

A

G

T

L

V

S

S

S
|
S

Q
|
Q

Y

Y

V

V

S

S

A

S

L

I

L

L

M

M

L

A

A

A

A

P

C

Y

G

A

E

H

A

Q

P

P

I

V

E

T

V

L

A

R

L

L

T

V

G

G

K

G

D

K

I

P

G

I

A

S

R

Q

G

P

Y
|
Y

V

I

D

D

L

M

T

T

L

I

D

A

C

M

M

M

R

A

A

S

F

F

G

G

A

I

Q

K

V

V

E

E

-

R

A

S

V

A

D

E

D

D

T

P

T

N

W

T

R

Y

V

L

A

I

P

P

T

K

G

G

Y

V

T

Y

A

K

H

N

-

P

D

E

Y

Y

L

V

I

V

E

E

P

S

D

D

A

A

S

S

A

S

A

A

T

T

Y

Y

L

P

W

L

A

A

A

V

E

A

V

A

L

I

T

T

G

G

G

T

R

T

I

C

D

T

I

I

G

P

V

N

A

I

A

G

Q

S

D

E

F

S

T

L

Q

Q

P

G

D

D

A

A

K

R

A

F

Q

A

A

V

V

E

I

V

-

L

-

R

-

P

-

M

-

G

-

C

-

A

A

V

Q

E

F

Q

P

T

N

A

M

T

Q

S

A

T

T

T

V

V

V

T

G

G

-

P

-

I

-

G

-

T

-

L

-

K

-

A

-

I

-

P

-

H

-

V

-

D

-

M

S

E

Q

P

M

M

Q

T

D

D

A

A

I

F

P

L

T

T

L

A

A

A

V

V

L

L

A

A

F

V

N

A

N

D

T

G

P

T

V

T

R

Q

F

I

T

T

E

G

L

I

A

A

N

N

L

Q

R

R

V

V

K

K

E

E

C

C

D

N

R

R

V

I

Q

A

A

A

L

M

H

K

D

D

G

Q

L

L

-

A

-

K

-

F

-

G

-

V

-

Q

-

C

N

N

E

E

I

L

R

E

P

D

G

G

L

I

A

E

T

V

I

I

E

-

G

G

D

K

D

P

L

Y

L

Q

V

E

A

L

S

R

D

N

P

P

A

-

L

V

A

E

G

G

T

I

S

Y

C

C

N

-

A

-

L

-

I

-

D

-

T

-

H

Y

A

D

D

D

H

H

R

R

I

V

A

A

M

M

C

S

F

H

A

S

-

V

L

L

A

S

G

T

L

I

K

S

V

P

A

H

G

P

I

V

R

L

I

I

Q

L

D

E

P

R

D

E

C

C

V

T

A

A

K

K

T

T

Y

W

P

P

E

G

Y

W

W

W

K

D

A

I

L

L

G

S

S

Q

-

F

L

F

G

K

V

V

Q

Q

L

L

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S409 (= S27), R413 (= R31), T483 (= T98), S562 (= S171), Q563 (= Q172), Y593 (= Y202)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1036, 1060, 1062, 1094, 1153, 1181, 1214, 1224, 1232, 1242, 1243, 1246
binding glutamic acid: 139, 145, 187, 243, 257, 260, 261, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 49, 74, 75, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 155, 172, 175, 176, 177, 180, 187, 243, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 874, 922, 923, 924, 979, 1269, 1277, 1279, 1321, 1322, 1323, 1327, 1348, 1368, 1526, 1530
binding zinc ion: 187, 264, 280

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
34% identity, 94% coverage: 21:412/418 of query aligns to 410:839/1583 of P07547

query
sites
P07547

A

A

P

P

G

G

S

S

K

K

S

S

I

I

T

S

N

N

R

R

A

A

L

L

L

V

L

L

A

A

L

L

A

G

N

S

G

G

T

T

S

C

R

R

L

I

S

K

G

N

A

L

L

L

K

H

S

S

D

D

T

T

R

E

H

V

M

M

S

L

V

N

A

A

L

L

R

E

Q

R

M

L

G

G

V

A

T

A

I

T

D

F

-

S

-

W

E

E

P

E

D

E

D

G

T

E

T

V

F

L

V

V

T

N

G

G

Q

K

-

G

G

G

K

N

L

L

Q

Q

L

A

P

S

S

S

Q

S

P

P

L

L

F

Y

L

L

G

G

N

N

A

A

G

G

T

T

A

A

M

S

R

R

F

F

L

L

T

T

-

T

-

V

-

A

-

T

-

L

A

A

A

N

V

S

A

S

T

T

V

V

E

D

G

S

T

S

V

-

V

V

L

L

D

T

G

G

D

N

Y

R

M

M

Q

K

K

Q

R

R

P

P

I

I

G

G

P

D

L

L

L

V

A

D

T

A

L

L

G

T

Q

A

N

N

G

G

I

A

L

S

V

I

D

E

-

Y

-

V

S

E

P

R

T

T

G

G

C

S

P

L

P

P

V

L

T

K

V

I

H

A

G

A

V

S

G

G

K

G

I

F

K

A

A

G

K

G

R

N

F

I

E

N

I

L

D

A

G

A

G

K

L

V

S

S

Q

Q

Y

Y

V

V

S

S

A

S

L

L

M

M

L

C

A

A

A

P

C

Y

G

A

E

K

A

E

P

P

I

V

E

T

V

L

A

R

L

L

T

V

G

G

K

G

D

K

I

P

G

I

A

S

R

Q

G

P

Y

Y

V

I

D

D

L

M

T

T

L

T

D

A

C

M

M

M

R

R

A

S

F

F

G

G

A

I

Q

D

V

V

E

Q

-

K

-

S

A

T

V

T

D

E

T

H

T

T

W

Y

R

H

V

I

A

-

P

P

T

Q

G

G

Y

R

T

Y

A

V

H

N

-

P

-

A

D

E

Y

Y

L

V

I

I

E

E

P

S

D

D

A

A

S

S

A

S

A

A

T

T

Y

Y

L

P

W

L

A

A

A

V

E

A

V

A

L

V

T

T

G

G

G

T

R

T

I

C

D

T

I

V

G

P

V

N

A

I

A

G

Q

S

D

A

F

S

T

L

Q

Q

P

G

D

D

A

A

K

R

-

F

-

A

-

V

-

E

-

V

-

L

-

R

-

P

-

M

-

G

-

C

-

T

-

V

-

E

-

Q

-

T

-

E

-

T

-

S

-

T

-

V

-

T

-

G

-

P

A

S

Q

D

A

G

V

I

I

L

A

R

Q

P

F

L

P

P

N

N

M

V

Q

D

A

M

T

-

V

-

G

-

S

E

Q

P

M

M

Q

T

D

D

A

A

I

F

P

L

T

G

L

A

A

S

V

V

L

L

A

A

F

I

-

A

-

R

-

G

-

K

-

E

N

S

N

N

T

H

P

T

V

T

R

R

F

I

T

Y

E

G

L

I

A

A

N

N

L

Q

R

R

V

V

K

K

E

E

C

C

D

N

R

R

V

I

Q

K

A

A

L

M

H

K

D

D

G

E

L

L

-

A

-

K

-

F

-

G

-

V

-

I

-

C

N

R

E

E

I

H

R

D

P

D

G

G

L

L

A

-

T

E

I

I

E

D

G

G

D

I

D

D

L

R

L

S

V

N

A

L

S

R

D

Q

P

P

A

-

L

V

A

G

G

G

T

V

S

F

C

C

N

-

A

-

L

-

I

-

D

-

T

-

H

Y

A

D

D

D

H

H

R

R

I

V

A

A

M

F

C

S

F

F

A

S

L

V

A

L

G

S

L

L

K

V

V

T

A

P

G

Q

-

P

I

T

R

L

I

I

Q

L

D

E

P

K

D

E

C

C

V

V

A

G

K

K

T

T

Y

W

P

P

E

G

Y

W

W

W

K

D

A

T

L

L

G

R

S

Q

L

L

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

P11043 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EC 2.5.1.19 from Petunia hybrida (Petunia) (see paper)
35% identity, 96% coverage: 9:409/418 of query aligns to 78:510/516 of P11043

query
sites
P11043

V

V

T

L

P

Q

P

P

N

I

F

K

P

E

L

I

N

S

G

G

K

T

V

V

A

K

P

L

P

P

G

G

S

S

K

K

S

S

I

L

T

S

N

N

R

R

A

I

L

L

A

A

L

L

A

S

N

E

G

G

T

T

S

T

R

V

L

V

S

D

G

N

A

L

L

L

K

S

S

S

D

D

T

I

R

H

H

Y

M

M

S

L

V

G

A

A

L

L

R

K

Q

T

M

L

G

G

V

L

T

H

I

V

D

E

E

E

P

D

D

S

-

A

D

N

T

Q

T

R

F

A

V

V

T

E

G

G

-

C

-

G

-

L

-

F

-

P

Q

V

G

G

K

K

L

E

Q

S

L

K

P

E

S

E

Q

I

P

Q

L

L

F

F

L

L

G

G

N

N

A

A

G
|
G

T

T

A

A

M

M

R

R

F

P

L

L

T

T

A

A

V

V

A

T

T

V

V

A

E

G

G

G

T

N

-

S

-

R

V

Y

V

V

L

L

D

D

G

G

D

V

D

P

Y

R

M

M

Q

R

K

E

R

R

P

P

I

I

G

S

P

D

L

L

L

V

A

D

T

G

L

L

G

K

Q

Q

N

L

G

G

I

A

L

E

V

V

D

D

S

C

P

F

T

L

G

G

-

T

-

K

C

C

P

P

V

V

T

R

V

I

H

V

G

S

V

K

G

G

K

G

I

L

K

P

A

G

K

G

R

K

F

V

E

K

I

L

D

S

G

G

G

S

L

I

S

S

Q

Q

Y

Y

V

L

S

T

A

A

L

L

M

M

L

A

A

A

A

P

C

L

G

A

E

L

A

G

P

D

I

V

E

E

V

I

A

E

L

I

T

I

G

D

K

K

D

L

I

I

G

S

A

V

R

P

G

-

Y

Y

V

V

D

E

L

M

T

T

L

L

D

K

C

L

M

M

R

E

A

R

F

F

G

G

A

I

Q

S

V

V

E

E

A

-

V

-

D

H

D

S

T

S

W

W

-

D

-

R

-

F

-

V

R

R

V

G

A

G

P

Q

T

K

G

Y

Y

K

T

S

A

P

H

G

D

K

Y

A

L

F

I

V

E

E

P

G

D

D

A

A

S

S

A

S

A

A

T

S

Y

Y

L

F

W

L

A

A

A

G

E

A

V

A

L

V

T

T

G

G

R

T

I

I

D

T

I

V

G

E

V

G

A

C

A

G

Q

T

D

N

F

S

T

L

Q

Q

P

G

D

D

A

V

K

K

A

F

Q

A

A

E

V

V

I

L

A

E

Q

K

-

M

-

G

-

A

-

E

-

V

-

T

F

W

P

T

N

E

M

N

Q

S

A

V

T

T

V

V

V

K

G

G

-

P

-

R

-

S

-

G

-

R

-

K

-

H

-

L

-

R

-

A

-

I

-

D

-

V

-

N

-

M

S

N

Q

K

M

M

Q

P

D

D

A

V

I

A

P

M

T

T

L

L

A

A

V

V

L

V

A

A

A

L

F

Y

N

A

N

D

T

G

P

P

V

T

R

A

F

I

T

R

E

D

L

V

A

A

N

S

L

W

R

R

V

V

K

K

E

E

C

T

D

E

R

R

V

M

Q

I

A

A

L

I

H

-

D

-

G

-

L

C

N

T

E

E

I

L

R

R

P

K

G

L

L

G

A

A

T

T

I

V

-

E

E

E

G

G

D

P

D

D

L

Y

L

C

V

I

A

I

S

T

D

P

P

P

A

E

L

K

A

L

G

N

T

V

S

T

C

D

N

-

A

-

L

-

I

I

D

D

T

T

H

Y

A

D

D

D

H

H

R

R

I

M

A

A

M

M

C

A

F

F

A

S

L

L

A

A

G

A

L

C

K

A

V

D

A

V

G

P

I

V

R

T

I

I

Q

N

D

D

P

P

D

G

C

C

V

T

A

R

K

K

T

T

Y

F

P

P

E

N

Y

Y

W

F

K

D

A

V

L

L

G173 (= G97) mutation to A: Resistance to glyphosate due to a lower affinity. Slight reduction in EPSP synthase activity.

2pq9A E. Coli epsps liganded with (r)-difluoromethyl tetrahedral reaction intermediate analog (see paper)
34% identity, 95% coverage: 16:412/418 of query aligns to 12:423/427 of 2pq9A

query
sites
2pq9A

L

V

N

D

G

G

K

T

V

I

A

N

P

L

P

P

G

G

S

S

K
|
K

S
|
S

I

V

T

S

N

N

R
|
R

A

A

L

L

A

A

L

L

A

A

N

H

G

G

T

K

S

T

R

V

L

L

S

T

G

N

A

L

L

L

K

D

S

S

D

D

D
|
D

T

V

R

R

H

H

M

M

S

L

V

N

A

A

L

L

R

T

Q

A

M

L

G

G

V

V

T

S

I

Y

D

T

E

L

P

S

D

A

D

D

T

R

T

T

F

R

-

C

-

E

V

I

T

G

G

N

Q

G

G

G

K

P

L

L

Q

H

L

A

P

E

-

G

S

A

Q

L

P

E

L

L

F

F

L

L

G

G

N
|
N

A
|
A

G
|
G

T
|
T

A

A

M

M

R

R

F

P

L

L

T

A

A

A

V

L

A

C

T

L

V

G

E

S

G

N

T

D

V

I

V

V

L

L

D

T

G

G

D

E

D
x
P

Y

R

M

M

Q

K

K

E

R
|
R

P

P

I

I

G

G

P

H

L

L

L

V

A

D

T

A

L

L

G

R

Q

L

N

G

G

G

-

A

-

K

I

I

L

T

V

Y

D

L

S

E

P

Q

T

E

G

N

C

Y

P

P

V

L

T

R

V

L

H

Q

G

-

V

-

G

G

K

G

I

F

K

T

A

G

K

G

R

N

F

V

E

D

I

V

D

D

G

G

G

S

L

V

S
|
S

Q
|
Q

Y

F

V

L

S

T

A

A

L

L

M

M

L

T

A

A

A

P

C

L

G

A

E

P

A

E

P

D

I

T

E

V

V

I

A

R

L

I

T

K

G

G

K

-

D

D

I

L

G

V

A
x
S

R

K

G

P

Y
|
Y

V

I

D

D

L

I

T

T

L

L

D

N

C

L

M

M

R

K

A

T

F

F

G

G

A

V

Q

E

V

I

E

E

A

N

V

Q

D

H

D

Y

T

Q

W

F

R

V

V

V

-

K

-

G

A

G

P

Q

T

S

G

Y

Y

Q

T

S

A

P

H

G

D

T

Y

Y

L

L

I

V

E

E

P

G

D

D

A

A

S

S

A

S

A

A

T

S

Y

Y

L

F

W

L

A

A

E

A

V

A

L

I

T

K

G

G

R

T

I

V

D

K

I

V

G

T

V

G

A

I

A

G

Q

R

D

N

F

S

T

M

Q

Q

P

G

D

D

A

I

K

R

A

F

Q

A

A

D

V

V

I

L

A

E

Q

K

F

-

P

-

N

-

M

M

Q

G

A

A

T

T

V

I

V

C

G

W

-

G

-

D

-

Y

-

I

-

S

-

C

-

T

-

R

-

G

-

E

-

L

-

N

-

A

-

I

-

D

-

M

-

D

-

M

S

N

Q

H

M

I

Q

P

D
|
D

A

A

I

A

P

M

T

T

L

I

A

A

V

T

L

A

A

A

A

L

F

F

N

A

N

K

T

G

P

T

V

T

R

T

F

L

T

R

E

N

L

I

A

Y

N
|
N

L

W

R

R

V

V

K
|
K

E
|
E

C

T

D

D

R
|
R

V

L

Q

F

A

A

L

M

H

A

D

T

G

E

L

L

N

R

E

K

I

V

R

-

P

-

G

G

L

A

A

E

T

V

I

E

E

E

G

G

D

H

D

D

L

Y

L

I

V

R

A

I

S

T

D

P

P

P

A

E

L

K

A

L

G

N

T

F

S

-

C

-

N

-

A

A

L

E

I

I

D

A

T

T

H

Y

A

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

C

C

F

F

A

S

L

L

A

V

G

A

L

L

K

S

V

D

A

T

G

P

I

V

R

T

I

I

Q

L

D

D

P

P

D

K

C

C

V

T

A

A

K
|
K

T

T

Y

F

P

P

E

D

Y

Y

W

F

K

E

A

Q

L

L

G

A

S

R

L

I

active site: K22 (= K26), S23 (= S27), D49 (= D53), N94 (= N95), P119 (≠ D120), R124 (= R125), D313 (= D297), E341 (= E325), H385 (= H374), R386 (= R375), K411 (= K400)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K22 (= K26), S23 (= S27), R27 (= R31), A95 (= A96), G96 (= G97), T97 (= T98), R124 (= R125), S169 (= S170), S170 (= S171), Q171 (= Q172), S197 (≠ A199), Y200 (= Y202), D313 (= D297), N336 (= N320), K340 (= K324), E341 (= E325), R344 (= R328), H385 (= H374), R386 (= R375), K411 (= K400)

2aa9A Epsp synthase liganded with shikimate (see paper)
34% identity, 95% coverage: 16:412/418 of query aligns to 12:423/427 of 2aa9A

query
sites
2aa9A

L

V

N

D

G

G

K

T

V

I

A

N

P

L

P

P

G

G

S

S

K
|
K

S
|
S

I

V

T

S

N

N

R
|
R

A

A

L

L

A

A

L

L

A

A

N

H

G

G

T

K

S

T

R

V

L

L

S

T

G

N

A

L

L

L

K

D

S

S

D

D

D
|
D

T

V

R

R

H

H

M

M

S

L

V

N

A

A

L

L

R

T

Q

A

M

L

G

G

V

V

T

S

I

Y

D

T

E

L

P

S

D

A

D

D

T

R

T

T

F

R

-

C

-

E

V

I

T

G

G

N

Q

G

G

G

K

P

L

L

Q

H

L

A

P

E

-

G

S

A

Q

L

P

E

L

L

F

F

L

L

G

G

N
|
N

A

A

G

G

T
|
T

A

A

M

M

R

R

F

P

L

L

T

A

A

A

V

L

A

C

T

L

V

G

E

S

G

N

T

D

V

I

V

V

L

L

D

T

G

G

D

E

D
x
P

Y

R

M

M

Q

K

K

E

R
|
R

P

P

I

I

G

G

P

H

L

L

L

V

A

D

T

A

L

L

G

R

Q

L

N

G

G

G

-

A

-

K

I

I

L

T

V

Y

D

L

S

E

P

Q

T

E

G

N

C

Y

P

P

V

L

T

R

V

L

H

Q

G

-

V

-

G

G

K

G

I

F

K

T

A

G

K

G

R

N

F

V

E

D

I

V

D

D

G

G

G

S

L

V

S

S

Q
|
Q

Y

F

V

L

S

T

A

A

L

L

M

M

L

T

A

A

A

P

C

L

G

A

E

P

A

E

P

D

I

T

E

V

V

I

A

R

L

I

T

K

G

G

K

-

D

D

I

L

G

V

A

S

R

K

G

P

Y
|
Y

V

I

D

D

L

I

T

T

L

L

D

N

C

L

M

M

R

K

A

T

F

F

G

G

A

V

Q

E

V

I

E

E

A

N

V

Q

D

H

D

Y

T

Q

W

F

R

V

V

V

-

K

-

G

A

G

P

Q

T

S

G

Y

Y

Q

T

S

A

P

H

G

D

T

Y

Y

L

L

I

V

E

E

P

G

D

D

A

A

S

S

A

S

A

A

T

S

Y

Y

L

F

W

L

A

A

E

A

V

A

L

I

T

K

G

G

R

T

I

V

D

K

I

V

G

T

V

G

A

I

A

G

Q

R

D

N

F

S

T

M

Q

Q

P

G

D

D

A

I

K

R

A

F

Q

A

A

D

V

V

I

L

A

E

Q

K

F

-

P

-

N

-

M

M

Q

G

A

A

T

T

V

I

V

C

G

W

-

G

-

D

-

Y

-

I

-

S

-

C

-

T

-

R

-

G

-

E

-

L

-

N

-

A

-

I

-

D

-

M

-

D

-

M

S

N

Q

H

M

I

Q

P

D
|
D

A

A

I

A

P

M

T

T

L

I

A

A

V

T

L

A

A

A

A

L

F

F

N

A

N

K

T

G

P

T

V

T

R

T

F

L

T

R

E

N

L

I

A

Y

N

N

L

W

R

R

V

V

K
|
K

E
|
E

C

T

D

D

R

R

V

L

Q

F

A

A

L

M

H

A

D

T

G

E

L

L

N

R

E

K

I

V

R

-

P

-

G

G

L

A

A

E

T

V

I

E

E

E

G

G

D

H

D

D

L

Y

L

I

V

R

A

I

S

T

D

P

P

P

A

E

L

K

A

L

G

N

T

F

S

-

C

-

N

-

A

A

L

E

I

I

D

A

T

T

H

Y

A

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

C

C

F

F

A

S

L

L

A

V

G

A

L

L

K

S

V

D

A

T

G

P

I

V

R

T

I

I

Q

L

D

D

P

P

D

K

C

C

V

T

A

A

K
|
K

T

T

Y

F

P

P

E

D

Y

Y

W

F

K

E

A

Q

L

L

G

A

S

R

L

I

active site: K22 (= K26), S23 (= S27), D49 (= D53), N94 (= N95), P119 (≠ D120), R124 (= R125), D313 (= D297), E341 (= E325), H385 (= H374), R386 (= R375), K411 (= K400)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: K22 (= K26), S23 (= S27), R27 (= R31), T97 (= T98), Q171 (= Q172), Y200 (= Y202), D313 (= D297), K340 (= K324)

1x8tA Epsps liganded with the (r)-phosphonate analog of the tetrahedral reaction intermediate (see paper)
34% identity, 95% coverage: 16:412/418 of query aligns to 12:423/427 of 1x8tA

query
sites
1x8tA

L

V

N

D

G

G

K

T

V

I

A

N

P

L

P

P

G

G

S

S

K
|
K

S
|
S

I

V

T

S

N

N

R
|
R

A

A

L

L

A

A

L

L

A

A

N

H

G

G

T

K

S

T

R

V

L

L

S

T

G

N

A

L

L

L

K

D

S

S

D

D

D
|
D

T

V

R

R

H

H

M

M

S

L

V

N

A

A

L

L

R

T

Q

A

M

L

G

G

V

V

T

S

I

Y

D

T

E

L

P

S

D

A

D

D

T

R

T

T

F

R

-

C

-

E

V

I

T

G

G

N

Q

G

G

G

K

P

L

L

Q

H

L

A

P

E

-

G

S

A

Q

L

P

E

L

L

F

F

L

L

G

G

N
|
N

A

A

G

G

T
|
T

A

A

M

M

R

R

F

P

L

L

T

A

A

A

V

L

A

C

T

L

V

G

E

S

G

N

T

D

V

I

V

V

L

L

D

T

G

G

D

E

D
x
P

Y

R

M

M

Q

K

K

E

R
|
R

P

P

I

I

G

G

P

H

L

L

L

V

A

D

T

A

L

L

G

R

Q

L

N

G

G

G

-

A

-

K

I

I

L

T

V

Y

D

L

S

E

P

Q

T

E

G

N

C

Y

P

P

V

L

T

R

V

L

H

Q

G

-

V

-

G

G

K

G

I

F

K

T

A

G

K

G

R

N

F

V

E

D

I

V

D

D

G

G

G

S

L

V

S
|
S