SitesBLAST
Comparing GFF2535 FitnessBrowser__WCS417:GFF2535 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
85% identity, 99% coverage: 1:318/321 of query aligns to 1:318/319 of A4F2N8
- K53 (= K53) mutation to A: Loss of enzymatic activity.
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
65% identity, 98% coverage: 4:319/321 of query aligns to 4:319/319 of 2zr8A
- active site: K53 (= K53), S78 (= S78), E204 (= E204), G208 (= G208), D210 (= D210), G232 (= G232), I303 (≠ V303), S304 (≠ T304)
- binding magnesium ion: E204 (= E204), G208 (= G208), D210 (= D210)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F52), K53 (= K53), S77 (= S77), S78 (= S78), N80 (= N80), H81 (= H81), P147 (= P147), G179 (= G179), G180 (= G180), G181 (= G181), G182 (= G182), G232 (= G232), E277 (= E277), T279 (= T279), S304 (≠ T304)
- binding serine: S78 (= S78), R129 (= R129), D231 (= D231), G232 (= G232), A233 (= A233), Q234 (= Q234), T235 (= T235)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
65% identity, 98% coverage: 4:319/321 of query aligns to 4:319/319 of 2zpuA
- active site: K53 (= K53), S78 (= S78), E204 (= E204), G208 (= G208), D210 (= D210), G232 (= G232), I303 (≠ V303), S304 (≠ T304)
- binding magnesium ion: E204 (= E204), G208 (= G208), D210 (= D210)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F52), K53 (= K53), S77 (= S77), S78 (= S78), N80 (= N80), H81 (= H81), P147 (= P147), G179 (= G179), G180 (= G180), G181 (= G181), G182 (= G182), G232 (= G232), E277 (= E277), T279 (= T279), S304 (≠ T304)
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
65% identity, 98% coverage: 4:319/321 of query aligns to 3:318/318 of 1wtcA
- active site: K52 (= K53), S77 (= S78), E203 (= E204), G207 (= G208), D209 (= D210), G231 (= G232), I302 (≠ V303), S303 (≠ T304)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ H21), K47 (≠ R48), M48 (≠ A49), A109 (= A110), A110 (= A111), Y114 (= Y115)
- binding magnesium ion: E203 (= E204), G207 (= G208), D209 (= D210)
- binding pyridoxal-5'-phosphate: F51 (= F52), K52 (= K53), N79 (= N80), G178 (= G179), G179 (= G180), G180 (= G181), G181 (= G182), G231 (= G232), E276 (= E277), T278 (= T279), S303 (≠ T304)
1v71A Crystal structure of s.Pombe serine racemase
65% identity, 98% coverage: 4:319/321 of query aligns to 3:318/318 of 1v71A
- active site: K52 (= K53), S77 (= S78), E203 (= E204), G207 (= G208), D209 (= D210), G231 (= G232), I302 (≠ V303), S303 (≠ T304)
- binding magnesium ion: E203 (= E204), G207 (= G208), D209 (= D210)
- binding pyridoxal-5'-phosphate: F51 (= F52), K52 (= K53), N79 (= N80), G178 (= G179), G179 (= G180), G180 (= G181), G181 (= G182), G231 (= G232), E276 (= E277), T278 (= T279), S303 (≠ T304), G304 (= G305)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
65% identity, 98% coverage: 4:319/321 of query aligns to 8:323/323 of O59791
- S82 (= S78) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
41% identity, 93% coverage: 11:310/321 of query aligns to 26:329/339 of Q7XSN8
- E219 (= E204) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (= D210) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
6zspAAA serine racemase bound to atp and malonate. (see paper)
40% identity, 98% coverage: 5:320/321 of query aligns to 5:319/320 of 6zspAAA
- active site: K53 (= K53), S74 (= S78), E200 (= E204), A204 (≠ G208), D206 (= D210), G229 (= G232), L302 (≠ V303), S303 (≠ T304)
- binding adenosine-5'-triphosphate: S28 (= S28), S29 (≠ R29), I30 (≠ T30), K48 (≠ R48), T49 (≠ A49), Q79 (= Q83), Y111 (= Y115), E266 (≠ Q270), R267 (= R271), K269 (= K273), N306 (= N307)
- binding magnesium ion: E200 (= E204), A204 (≠ G208), D206 (= D210)
- binding malonate ion: K53 (= K53), S73 (= S77), S74 (= S78), N76 (= N80), H77 (= H81), R125 (= R129), G229 (= G232), S232 (≠ T235)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/320 of 7nbhAAA
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S78), G85 (≠ A82), Q86 (= Q83), K111 (= K108), I115 (≠ T112), Y118 (= Y115), D235 (= D231), P281 (= P278), N313 (= N307), V314 (= V308), D315 (= D309)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/323 of 7nbfAAA
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding magnesium ion: N244 (≠ Y241)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N83 (= N80), G182 (= G179), G183 (= G180), G184 (= G181), G185 (= G182), M186 (= M183), G236 (= G232), V237 (≠ A233), T282 (= T279), S310 (≠ T304), G311 (= G305)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (= H21), L22 (≠ A22), T23 (= T23), P24 (= P24), L26 (≠ F26), T27 (= T27), F46 (≠ L46)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/323 of 7nbdAAA
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ M269), L278 (≠ V275), V314 (= V308), L316 (≠ I310)
- binding magnesium ion: N244 (≠ Y241)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N83 (= N80), G182 (= G179), G183 (= G180), G184 (= G181), G185 (= G182), M186 (= M183), G236 (= G232), V237 (≠ A233), E280 (= E277), T282 (= T279), S310 (≠ T304), G311 (= G305)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/323 of 7nbcCCC
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding biphenyl-4-ylacetic acid: T78 (≠ A75), H79 (≠ F76), H84 (= H81), V148 (≠ I145), H149 (≠ P146), P150 (= P147)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N83 (= N80), G182 (= G179), G183 (= G180), G184 (= G181), G185 (= G182), M186 (= M183), G236 (= G232), V237 (≠ A233), T282 (= T279), S310 (≠ T304), G311 (= G305)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/323 of 7nbcAAA
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding magnesium ion: N244 (≠ Y241)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N83 (= N80), G182 (= G179), G183 (= G180), G184 (= G181), G185 (= G182), M186 (= M183), G236 (= G232), V237 (≠ A233), T282 (= T279), S310 (≠ T304), G311 (= G305)
Sites not aligning to the query:
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 5:316/322 of 7nbgAAA
- active site: K53 (= K53), S81 (= S78), E207 (= E204), A211 (≠ G208), D213 (= D210), G236 (= G232), L309 (≠ V303), S310 (≠ T304)
- binding calcium ion: E207 (= E204), A211 (≠ G208), D213 (= D210)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N83 (= N80), G182 (= G179), G183 (= G180), G184 (= G181), G185 (= G182), M186 (= M183), G236 (= G232), V237 (≠ A233), T282 (= T279), S310 (≠ T304), G311 (= G305)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S78), G85 (≠ A82), Q86 (= Q83), I101 (= I98), K111 (= K108), I115 (≠ T112), Y118 (= Y115)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
39% identity, 98% coverage: 5:320/321 of query aligns to 6:322/322 of 3l6bA
- active site: K54 (= K53), S77 (= S78), E203 (= E204), A207 (≠ G208), D209 (= D210), G232 (= G232), T278 (= T279), L305 (≠ V303), S306 (≠ T304)
- binding malonate ion: K54 (= K53), S76 (= S77), S77 (= S78), N79 (= N80), H80 (= H81), R128 (= R129), G232 (= G232)
- binding manganese (ii) ion: E203 (= E204), A207 (≠ G208), D209 (= D210)
- binding pyridoxal-5'-phosphate: F53 (= F52), K54 (= K53), N79 (= N80), G178 (= G179), G179 (= G180), G180 (= G181), G181 (= G182), M182 (= M183), V233 (≠ A233), E276 (= E277), T278 (= T279), S306 (≠ T304), G307 (= G305)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
40% identity, 95% coverage: 5:309/321 of query aligns to 5:310/310 of 7nbgDDD
- active site: K53 (= K53), S76 (= S78), E202 (= E204), A206 (≠ G208), D208 (= D210), G231 (= G232), L304 (≠ V303), S305 (≠ T304)
- binding calcium ion: E202 (= E204), A206 (≠ G208), D208 (= D210)
- binding magnesium ion: N239 (≠ Y241)
- binding ortho-xylene: S76 (= S78), Q81 (= Q83), I96 (= I98), Y113 (= Y115)
- binding pyridoxal-5'-phosphate: F52 (= F52), K53 (= K53), N78 (= N80), G177 (= G179), G178 (= G180), G179 (= G181), G180 (= G182), M181 (= M183), G231 (= G232), V232 (≠ A233), E275 (= E277), T277 (= T279), S305 (≠ T304), G306 (= G305)
Sites not aligning to the query:
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
46% identity, 96% coverage: 4:310/321 of query aligns to 1:306/308 of 1ve5A
- active site: K50 (= K53), S56 (≠ N59), S72 (= S78), E200 (= E204), A204 (≠ G208), D206 (= D210), G229 (= G232), L299 (≠ V303), S300 (≠ T304)
- binding calcium ion: E200 (= E204), A204 (≠ G208), D206 (= D210)
- binding pyridoxal-5'-phosphate: F49 (= F52), K50 (= K53), N74 (= N80), G175 (= G179), G176 (= G180), G177 (= G181), G178 (= G182), E274 (= E277), T276 (= T279), S300 (≠ T304), G301 (= G305)
5cvcA Structure of maize serine racemase (see paper)
41% identity, 94% coverage: 8:310/321 of query aligns to 6:313/329 of 5cvcA
- active site: K52 (= K53), S77 (= S78), E203 (= E204), A207 (≠ G208), D209 (= D210), G231 (= G232), V306 (= V303), S307 (≠ T304)
- binding magnesium ion: E203 (= E204), A207 (≠ G208), D209 (= D210)
- binding pyridoxal-5'-phosphate: F51 (= F52), K52 (= K53), N79 (= N80), S178 (≠ G179), G179 (= G180), G180 (= G181), G181 (= G182), L232 (≠ A233), E275 (= E277), S307 (≠ T304), G308 (= G305)
Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
40% identity, 95% coverage: 5:310/321 of query aligns to 8:319/339 of Q9QZX7
- C113 (≠ A107) modified: S-nitrosocysteine; mutation to S: Abolishes S-nitrosylation.
3hmkA Crystal structure of serine racemase (see paper)
39% identity, 95% coverage: 5:310/321 of query aligns to 6:317/321 of 3hmkA
- active site: K54 (= K53), S82 (= S78), E208 (= E204), A212 (≠ G208), D214 (= D210), G237 (= G232), T283 (= T279), L310 (≠ V303), S311 (≠ T304)
- binding manganese (ii) ion: E208 (= E204), A212 (≠ G208), D214 (= D210)
- binding pyridoxal-5'-phosphate: F53 (= F52), K54 (= K53), N84 (= N80), G183 (= G179), G184 (= G180), G185 (= G181), G186 (= G182), M187 (= M183), G237 (= G232), V238 (≠ A233), T283 (= T279), S311 (≠ T304), G312 (= G305)
Query Sequence
>GFF2535 FitnessBrowser__WCS417:GFF2535
MPSPTYQDVIDAAQRLEGVAHATPVFTSRTLDALTGAQVFIKCEHLQRAGSFKFRGAFNA
LSQFNPQQRKAGVVAFSSGNHAQGIALAAQLLGIPATIVMPTDAPAAKVAATREYGASVV
LYDRFTEDREQIGRNLATEQGLTLIPPYDHPHILAGQGTAAKELLESTGPLDALFVGLGG
GGMLSGTALSTRALSPDCLLYGVEPEAGNDGQRSFRSGSIVHIDTPKTIADGAQTQHLGN
YTFPIIRDNVNDILTVSDAELVAAMKFFMQRMKMVVEPTGCLGLAALLNEAGRFKGQRVG
IIVTGGNVDIEKYAALLSEAS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory