SitesBLAST
Comparing GFF2578 FitnessBrowser__WCS417:GFF2578 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 15 hits to proteins with known functional sites (download)
7cdyA Crystal structure of glucose dehydrogenase
66% identity, 89% coverage: 38:381/385 of query aligns to 2:344/346 of 7cdyA
2g8sA Crystal structure of the soluble aldose sugar dehydrogenase (asd) from escherichia coli in the apo-form (see paper)
62% identity, 90% coverage: 36:380/385 of query aligns to 2:345/348 of 2g8sA
P75804 Aldose sugar dehydrogenase YliI; Asd; Soluble aldose sugar dehydrogenase YliI; EC 1.1.5.- from Escherichia coli (strain K12) (see paper)
60% identity, 95% coverage: 16:380/385 of query aligns to 8:367/371 of P75804
- Q82 (= Q94) binding pyrroloquinoline quinone
- E240 (= E253) binding Ca(2+)
- Y250 (= Y263) binding Ca(2+)
- Y261 (= Y274) binding pyrroloquinoline quinone
7cgzA Glucose dehydrogenase
60% identity, 89% coverage: 38:381/385 of query aligns to 2:319/321 of 7cgzA
2ismB Crystal structure of the putative oxidoreductase (glucose dehydrogenase) (ttha0570) from thermus theromophilus hb8
38% identity, 87% coverage: 42:375/385 of query aligns to 7:318/333 of 2ismB
3a9hA Crystal structure of pqq-dependent sugar dehydrogenase holo-form (see paper)
36% identity, 87% coverage: 40:375/385 of query aligns to 7:319/338 of 3a9hA
- active site: H120 (= H160), D139 (≠ E179), R182 (= R227), T224 (= T269), K226 (vs. gap), G228 (= G271)
- binding calcium ion: E208 (= E253), Y218 (= Y263)
- binding alpha-D-glucopyranose: G228 (= G271), R229 (≠ I272), F232 (≠ S275), V233 (≠ L276), D234 (≠ T277)
- binding pyrroloquinoline quinone: E57 (≠ Q94), H120 (= H160), N183 (= N228), Q185 (= Q230), H201 (= H246), V204 (≠ R249), T243 (= T286), L269 (= L326), R270 (≠ A327), R298 (= R354), R300 (= R356)
Sites not aligning to the query:
3a9gA Crystal structure of pqq-dependent sugar dehydrogenase apo-form (see paper)
36% identity, 87% coverage: 40:375/385 of query aligns to 7:319/338 of 3a9gA
- active site: H120 (= H160), D139 (≠ E179), R182 (= R227), T224 (= T269), K226 (vs. gap), G228 (= G271)
- binding calcium ion: E208 (= E253), Y218 (= Y263)
- binding alpha-D-glucopyranose: R144 (≠ P184), D148 (= D188), G228 (= G271), R229 (≠ I272), F232 (≠ S275), V233 (≠ L276), D234 (≠ T277)
P13650 Quinoprotein glucose dehydrogenase B; Glucose dehydrogenase B [pyrroloquinoline-quinone]; Soluble glucose dehydrogenase; s-GDH; EC 1.1.5.2 from Acinetobacter calcoaceticus (see paper)
27% identity, 98% coverage: 1:376/385 of query aligns to 5:453/478 of P13650
- Q100 (= Q94) binding D-glucose
- D167 (≠ N159) binding D-glucose
- Q192 (vs. gap) binding D-glucose
- R252 (= R227) binding D-glucose
- G271 (= G247) binding Ca(2+)
- P272 (= P248) binding Ca(2+)
- E277 (= E253) binding Ca(2+)
- Y287 (= Y263) binding Ca(2+)
- A293 (vs. gap) binding Ca(2+)
- Y295 (vs. gap) binding Ca(2+)
- D297 (vs. gap) binding Ca(2+)
- E333 (≠ A282) binding Ca(2+)
- Y367 (≠ F308) binding pyrroloquinoline quinone
- T372 (≠ R313) binding pyrroloquinoline quinone
- K401 (≠ A327) binding pyrroloquinoline quinone
1cruA Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqq and methylhydrazine (see paper)
27% identity, 94% coverage: 17:376/385 of query aligns to 2:427/448 of 1cruA
- active site: H142 (= H160), D161 (≠ E179), R226 (= R227), A267 (vs. gap), Y269 (vs. gap), D271 (vs. gap), E307 (≠ A282)
- binding calcium ion: G245 (= G247), P246 (= P248), E251 (= E253), Y261 (= Y263), A267 (vs. gap), Y269 (vs. gap), D271 (vs. gap), E307 (≠ A282)
- binding methylhydrazine: Y341 (≠ F308), W344 (≠ A311)
- binding pyrroloquinoline quinone: Q76 (= Q94), H142 (= H160), R226 (= R227), N227 (= N228), Q244 (≠ H246), Y341 (≠ F308), T346 (≠ R313), A348 (≠ K315), L374 (= L326), K375 (≠ A327), R404 (= R354), R406 (= R356)
3dasA Structure of the pqq-bound form of aldose sugar dehydrogenase (adh) from streptomyces coelicolor
30% identity, 90% coverage: 31:375/385 of query aligns to 2:317/334 of 3dasA
- active site: H127 (= H160), E146 (= E179), R189 (= R227), E230 (≠ K283), K232 (= K285), G234 (≠ T286)
- binding alpha-L-arabinopyranose: K199 (≠ S238), D248 (vs. gap), R268 (≠ A327), G269 (≠ T328), E270 (≠ Q329), K278 (≠ D337), G279 (= G338)
- binding calcium ion: G208 (= G247), Q209 (≠ P248), E214 (= E253), Y224 (= Y263)
- binding pyrroloquinoline quinone: E66 (≠ Q94), H127 (= H160), R189 (= R227), N190 (= N228), Q192 (= Q230), F207 (≠ H246), S251 (= S300), R268 (≠ A327), R296 (= R354), R298 (= R356)
Sites not aligning to the query:
8re0A Quinoprotein glucose dehydrogenase B (see paper)
27% identity, 94% coverage: 17:376/385 of query aligns to 2:429/452 of 8re0A
- binding 3-(3,5-dicarboxy-1~{H}-pyrrol-2-yl)pyridine-2,4,6-tricarboxylic acid: R228 (= R227), Q231 (= Q230), Q246 (≠ H246), T348 (≠ R313), L376 (= L326), K377 (≠ A327), R406 (= R354), R408 (= R356)
- binding calcium ion: G247 (= G247), P248 (= P248), E253 (= E253), Y263 (= Y263), A269 (vs. gap), Y271 (vs. gap), D273 (vs. gap), E309 (≠ A282)
1cq1A Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqqh2 and glucose (see paper)
27% identity, 94% coverage: 17:376/385 of query aligns to 2:423/444 of 1cq1A
- active site: H138 (= H160), D157 (≠ E179), R222 (= R227), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A282)
- binding beta-D-glucopyranose: D137 (≠ N159), H138 (= H160), Q162 (vs. gap), Y337 (≠ F308)
- binding calcium ion: G241 (= G247), P242 (= P248), E247 (= E253), Y257 (= Y263), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A282)
- binding pyrroloquinoline quinone: Q76 (= Q94), H138 (= H160), R222 (= R227), N223 (= N228), Q240 (≠ H246), Y337 (≠ F308), T342 (≠ R313), A344 (≠ K315), L370 (= L326), K371 (≠ A327), R400 (= R354), R402 (= R356)
1c9uA Crystal structure of the soluble quinoprotein glucose dehydrogenase in complex with pqq (see paper)
27% identity, 94% coverage: 17:376/385 of query aligns to 2:423/444 of 1c9uA