SitesBLAST
Comparing GFF2583 Psest_2633 choline dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 95% coverage: 2:531/557 of query aligns to 4:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (= A13), E35 (= E33), A36 (= A34), W47 (= W62), P65 (≠ G80), G67 (= G82), V180 (≠ T222), A214 (≠ G257), G215 (= G258), A218 (= A261), T270 (≠ S334), Y391 (= Y471), A424 (= A504), I435 (≠ L515), N436 (= N516)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 97% coverage: 2:539/557 of query aligns to 23:578/578 of 5nccA
- active site: R347 (≠ P323), L420 (≠ V385), I421 (≠ G386), S507 (≠ A470), A509 (≠ H472), G552 (= G513), Q553 (≠ N514)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (= A13), L53 (= L32), E54 (= E33), A55 (= A34), F74 (≠ L55), W80 (= W62), A98 (≠ G80), G100 (= G82), G105 (= G87), S106 (= S88), N110 (= N92), A111 (≠ G93), T112 (≠ M94), L113 (≠ C95), V238 (≠ T222), A278 (≠ G257), H282 (≠ A261), L286 (≠ I265), N508 (≠ Y471), Q553 (≠ N514), T554 (≠ L515), G555 (≠ N516), V558 (≠ T519)
6yrvAAA structure of fap after illumination at 100k (see paper)
33% identity, 97% coverage: 2:541/557 of query aligns to 7:571/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H362), N499 (≠ Y471)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (= A13), L37 (= L32), E38 (= E33), A39 (= A34), F58 (≠ L55), W64 (= W62), A82 (≠ G80), G89 (= G87), S90 (= S88), N94 (= N92), A95 (≠ G93), T96 (≠ M94), L97 (≠ C95), M191 (≠ V191), V222 (≠ T222), C264 (= C256), A265 (≠ G257), G266 (= G258), H269 (≠ A261), N499 (≠ Y471), A534 (= A504), Q544 (≠ N514), T545 (≠ L515), G546 (≠ N516)
- binding heptadecane: V377 (≠ L364), G379 (vs. gap), M380 (≠ V366), G386 (= G372), T389 (≠ A375), Y390 (≠ H376), F393 (≠ H379), T408 (vs. gap), Q410 (vs. gap)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
33% identity, 96% coverage: 2:536/557 of query aligns to 83:642/654 of A0A248QE08
- TA 93:94 (≠ SA 12:13) binding
- E114 (= E33) binding
- L162 (≠ G84) binding
- S166 (= S88) binding
- NATL 170:173 (≠ NGMC 92:95) binding
- V298 (≠ T222) binding
- C432 (≠ F342) binding
- R451 (≠ H362) binding
- Y466 (≠ H376) binding
- Q486 (vs. gap) binding
- G622 (≠ N516) binding
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
34% identity, 95% coverage: 2:531/557 of query aligns to 13:527/530 of 3ljpA
- active site: I333 (≠ A344), P377 (≠ A383), N378 (≠ H384), A464 (= A470), H466 (= H472), V509 (≠ G513), N510 (= N514)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), A232 (≠ T222), T269 (≠ G257), D273 (≠ A261), Y465 (= Y471), H466 (= H472), D499 (= D503), A500 (= A504), N510 (= N514), P511 (≠ L515), N512 (= N516), V515 (≠ T519)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
34% identity, 95% coverage: 2:531/557 of query aligns to 13:527/532 of 4mjwA
- active site: I333 (≠ A344), P377 (≠ A383), N378 (≠ H384), V464 (≠ A470), H466 (= H472), V509 (≠ G513), N510 (= N514)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), R231 (≠ V221), A232 (≠ T222), T269 (≠ G257), G270 (= G258), D273 (≠ A261), Y465 (= Y471), H466 (= H472), A500 (= A504), N510 (= N514), P511 (≠ L515), N512 (= N516), V515 (≠ T519)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
34% identity, 95% coverage: 2:531/557 of query aligns to 13:527/527 of 2jbvA
- active site: I333 (≠ A344), P377 (≠ A383), N378 (≠ H384), V464 (≠ A470), H466 (= H472), V509 (≠ G513), N510 (= N514)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), R231 (≠ V221), A232 (≠ T222), T269 (≠ G257), G270 (= G258), D273 (≠ A261), V464 (≠ A470), Y465 (= Y471), H466 (= H472), D499 (= D503), A500 (= A504), N510 (= N514), P511 (≠ L515), N512 (= N516), V515 (≠ T519)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 94% coverage: 4:527/557 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V385), G361 (= G386), H444 (≠ A470), H446 (= H472), G487 (= G513), P488 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W62), R77 (= R81), G78 (= G82), R79 (≠ K83), G83 (= G87), S84 (= S88), H88 (≠ N92), A89 (≠ G93), G91 (≠ C95), R217 (≠ V221), V218 (≠ T222), A251 (≠ G257), E255 (≠ A261), H445 (≠ Y471), A478 (= A504), P488 (≠ N514), I489 (≠ L515), H490 (≠ N516)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 94% coverage: 4:527/557 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V385), G361 (= G386), H444 (≠ A470), H446 (= H472), G487 (= G513), P488 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W62), R77 (= R81), G78 (= G82), G83 (= G87), S84 (= S88), L87 (≠ I91), H88 (≠ N92), A89 (≠ G93), M90 (= M94), G91 (≠ C95), V218 (≠ T222), A251 (≠ G257), G252 (= G258), E255 (≠ A261), H445 (≠ Y471), A478 (= A504), P488 (≠ N514), I489 (≠ L515), H490 (≠ N516)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G93), S314 (≠ A316), H444 (≠ A470), H446 (= H472)
8bxlB Patulin synthase from penicillium expansum
33% identity, 95% coverage: 3:532/557 of query aligns to 14:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), T23 (≠ S12), A24 (= A13), E44 (= E33), A45 (= A34), W80 (= W62), G100 (= G82), G105 (= G87), S106 (= S88), R109 (≠ I91), N110 (= N92), Y111 (≠ G93), A113 (≠ C95), L253 (≠ V221), A254 (≠ T222), A288 (≠ G257), Q292 (≠ A261), F525 (≠ Y471), D559 (= D503), A560 (= A504), H570 (≠ N514), P571 (≠ L515), Q572 (≠ N516), L575 (≠ T519)
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
31% identity, 95% coverage: 3:530/557 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ F342), F364 (≠ V385), W365 (≠ G386), V461 (≠ A470), H463 (= H472), A506 (≠ G513), N507 (= N514)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W64 (= W62), G88 (= G82), G93 (= G87), G94 (≠ S88), N98 (= N92), M99 (≠ G93), V101 (≠ C95), V229 (≠ T222), T261 (≠ C256), A262 (≠ G257), W462 (≠ Y471), H463 (= H472), A497 (= A504), N507 (= N514), T508 (≠ L515), N509 (= N516), T512 (= T519)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
31% identity, 95% coverage: 3:530/557 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I91) mutation to H: Abolishes activity.
- M103 (≠ G93) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ H384) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ G386) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A470) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y471) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H472) mutation to A: Abolishes activity.
- N511 (= N514) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
29% identity, 91% coverage: 1:509/557 of query aligns to 39:576/602 of Q3L245
- N100 (≠ A63) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I91) modified: Tele-8alpha-FAD histidine
- N344 (= N294) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H472) active site, Proton acceptor
Sites not aligning to the query:
- 1:25 signal peptide
- 581 active site
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
29% identity, 91% coverage: 1:509/557 of query aligns to 14:551/577 of 4h7uA
- active site: A343 (≠ P315), V426 (≠ G386), Y510 (≠ A470), H512 (= H472)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ S12), A26 (= A13), E46 (= E33), A47 (= A34), W74 (= W62), G99 (= G87), C100 (≠ S88), H103 (≠ I91), N104 (= N92), G105 (= G93), V107 (≠ C95), L242 (≠ V221), V243 (≠ T222), G282 (= G257), G283 (= G258), A286 (= A261), H512 (= H472), A546 (= A504)
Sites not aligning to the query:
- active site: 555, 556
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): 556, 557, 558, 561
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
28% identity, 95% coverage: 3:531/557 of query aligns to 4:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y5), L22 (= L21), N25 (≠ D24), Y51 (≠ A49), I349 (= I325), Q356 (≠ L332), E411 (≠ H384), E444 (= E417), W445 (≠ Q418), K448 (≠ R421), R499 (≠ E468), N501 (≠ A470), H546 (≠ N514), K563 (≠ R531)
- binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (≠ A49), F55 (≠ Y53), W61 (= W62), R79 (≠ G80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), M93 (= M94), A94 (≠ C95), T232 (≠ V221), A233 (≠ T222), A273 (≠ G257), G274 (= G258), R277 (≠ A261), F502 (≠ Y471), A536 (= A504), H546 (≠ N514), L547 (= L515), V548 (≠ N516), L551 (≠ T519)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
28% identity, 95% coverage: 3:531/557 of query aligns to 4:563/569 of 4ynuA
- active site: V341 (vs. gap), F412 (≠ V385), W413 (≠ G386), N501 (≠ A470), H503 (= H472), G545 (= G513), H546 (≠ N514)
- binding flavin-adenine dinucleotide: G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (≠ A49), F55 (≠ Y53), W61 (= W62), R79 (≠ G80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), T232 (≠ V221), A233 (≠ T222), A273 (≠ G257), G274 (= G258), R277 (≠ A261), F502 (≠ Y471), A536 (= A504), H546 (≠ N514), L547 (= L515), V548 (≠ N516), L551 (≠ T519)
- binding D-glucono-1,5-lactone: Y51 (≠ A49), E411 (≠ H384), A496 (≠ E465), N497 (≠ H466), R499 (≠ E468), R499 (≠ E468), N501 (≠ A470), H503 (= H472), H546 (≠ N514)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
28% identity, 95% coverage: 3:531/557 of query aligns to 5:564/570 of 4yntA
- active site: V342 (vs. gap), F413 (≠ V385), W414 (≠ G386), N502 (≠ A470), H504 (= H472), G546 (= G513), H547 (≠ N514)
- binding dihydroflavine-adenine dinucleotide: G13 (= G11), T14 (≠ S12), S15 (≠ A13), E35 (= E33), A36 (= A34), F56 (≠ Y53), W62 (= W62), R80 (≠ G80), G82 (= G82), G87 (= G87), T88 (≠ S88), N92 (= N92), G93 (= G93), M94 (= M94), A95 (≠ C95), A234 (≠ T222), A274 (≠ G257), R278 (≠ A261), F503 (≠ Y471), A537 (= A504), H547 (≠ N514), L548 (= L515), V549 (≠ N516), L552 (≠ T519)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
28% identity, 96% coverage: 2:534/557 of query aligns to 1:565/565 of 5oc1A
- active site: V339 (≠ P315), N413 (≠ G386), A414 (≠ S387), I499 (≠ A470), H501 (= H472), A544 (≠ G513), H545 (≠ N514)
- binding 4-methoxybenzoic acid: Y91 (≠ G93), I356 (vs. gap), I390 (≠ E355), F396 (= F361), T412 (≠ V385), I499 (≠ A470), H501 (= H472), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W62), P78 (≠ G80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ C95), V230 (≠ T222), S270 (≠ C256), A271 (≠ G257), G272 (= G258), F500 (≠ Y471), H545 (≠ N514), T546 (≠ L515), Q547 (≠ N516), I550 (≠ T519)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
27% identity, 96% coverage: 2:534/557 of query aligns to 1:565/565 of 3fimB
- active site: V339 (≠ P315), N413 (≠ G386), A414 (≠ S387), I499 (≠ A470), H501 (= H472), A544 (≠ G513), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W62), P78 (≠ G80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ C95), V230 (≠ T222), S270 (≠ C256), A271 (≠ G257), F500 (≠ Y471), H501 (= H472), H545 (≠ N514), T546 (≠ L515), Q547 (≠ N516), I550 (≠ T519)
3q9tA Crystal structure analysis of formate oxidase (see paper)
27% identity, 95% coverage: 3:532/557 of query aligns to 7:571/577 of 3q9tA
- active site: A335 (= A324), D422 (≠ G386), A508 (= A470), H510 (= H472), C552 (≠ G513), R553 (≠ N514)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G11), T16 (≠ S12), E37 (= E33), A38 (= A34), W65 (= W62), T85 (≠ G80), R86 (= R81), G87 (= G82), G92 (= G87), S93 (= S88), N97 (= N92), Y98 (≠ G93), F99 (≠ M94), T100 (≠ C95), S229 (≠ T222), S265 (≠ C256), Q266 (≠ G257), E270 (≠ A261), F509 (≠ Y471), D542 (= D503), A543 (= A504), R553 (≠ N514), I554 (≠ L515), Q555 (≠ N516), V558 (≠ T519)
Query Sequence
>GFF2583 Psest_2633 choline dehydrogenase
MEYDYIIIGAGSAGNVLAARLTEDADVSVLLLEAGGPDYRLDFRTQMPAALAYPLQGTRY
NWAYKTDPEPHMNNRRMDCGRGKGLGGSSLINGMCYIRGNALDYDNWAKAPGLEDWTYLD
CLPYFRKAESRDIGPNDYHGGVGPVSVTTPRADNNVLFQAMVEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQARERPNLTIETHAVTDRVLFEGKRAVGVRYLRD
RKDPYIARARREVLLCGGAIASPQILQRSGVGPGALLRRVGVKPVHELPGVGQNLQDHLE
MYLQYECKQPVSLYPALKWWNQPAIGAEWLFLGSGIGASNQFEAGGFIRSSDEFEWPNIQ
FHFLPVAVSYNGSNAHDGHSFQAHVGSMRSPSRGRIEIRSTDPAEDPSILFNYMAHEQDW
REFRDGIRLTREIMNQPALDPYRGRELSPGIEAQSDAELDAFVREHAETAYHPSCSCKMG
TDDMAVVDGQGRVHGMEGLRVVDASIMPQIITGNLNATTIMIAEKIADRIRGREPLRRST
AKYYVAGNVPVRQPPRR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory