SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2621 FitnessBrowser__Phaeo:GFF2621 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
50% identity, 65% coverage: 100:346/378 of query aligns to 2:252/271 of Q72J02

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C192 (= C286) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
45% identity, 65% coverage: 105:350/378 of query aligns to 65:322/482 of O59954

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G82 (= G122) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G140) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R170) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G225) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E255) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G297) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
40% identity, 70% coverage: 84:348/378 of query aligns to 42:311/453 of Q9VLJ8

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T62 (vs. gap) modified: Phosphothreonine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
46% identity, 68% coverage: 104:361/378 of query aligns to 8:264/270 of D4GSF3

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C188 (= C286) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
40% identity, 71% coverage: 79:348/378 of query aligns to 17:290/440 of P38820

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Y

Y

L

L

V

V

N

S

R

D

T

V

A

A

V

V

A

N

L

L

G

G

K

I

P

T

L

V

I

V

S

S

G

A

A

S

L

G

S

L

Q

G

W

T

E

E

G

G

Q

Q

I

L

S

T

L

I

F

L

H

N

P

F

T

N

A

N

G

I

G

G

P

P

C

C

Y

Y

Q

R

C

C

I

F

F

Y

P

P

E

T

A

P

P

P

A

P

G

N

L

A

A

V

P

T

S

S

C
|
C

S

Q

E

E

A

G

G

G

V

V

V

I

G

G

P

P

L

C

P

I

G

G

V

L

V

V

G

G

A

T

M

M

A

A

L

V

E

E

A

T

I

L

K

K

A

L

V

I

T

L

G

G

-

I

-

Y

A

T

G

N

Q

E

T

N

L

F

Q

S

G

P

E

F

I

L

L

M

I

L

Y

Y

D

S

G

G

L

F

Y

P

S

Q

E

Q

S

S

-

L

R

R

K

T

I

F

T

K

L

M

S

R

Q

G

R

R

S

Q

D

E

C

K

P

C

I

L

C

C

A

C

G

G

C225 (= C286) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 65% coverage: 100:346/378 of query aligns to 3:247/249 of P12282

query
sites
P12282

K

E

F

L

S

S

E

D

S

Q

E

E

L

M

D

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

R

R

E

G

L

F

G

D

G

F

P

D

G

G

Q

Q

K

E

K

A

L

L

R

K

K

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A
x
C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

G

T

V

L

I

L

D

D

D

F

D

D

L

T

V

V

E

S

N

L

A

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

R

S

D

D

Q

A

D

T

I

V

G

G

I

Q

P

P

K

K

V

V

F

E

S

S

A

A

Q

R

A

D

A

A

M

L

E

T

A

R

Q

I

N

N

P

P

Y

H

V

I

T

A

V

I

R

T

P

P

Y

V

H

N

R

A

R

L

L

L

D

D

A

D

D

A

I

E

A

L

T

A

D

A

L

L

F

I

G

A

D

E

Y

H

D

D

L

L

I

V

L

L

D

D

G
x
C

T

T

D
|
D

N

N

F

V

E

A

T

V

R

R

Y

N

L

Q

V

L

N

N

R

A

T

G

A
x
C

V

F

A

A

L

A

G

K

K

V

P

P

L

L

I

V

S

S

G

G

A

A

L

A

S

I

Q

R

W

M

E

E

G

G

Q

Q

I

I

S

T

L

V

F

F

H

T

P

Y

T

Q

A

D

G

G

G

E

P

P

C
|
C

Y

Y

Q

R

C
|
C

-

L

-

S

-

R

I

L

F

F

P

G

E

E

A

N

P

-

A

-

G

-

L

-

A

A

P

L

S

T

C
|
C

S

V

E

E

A

A

G

G

V

V

V

M

G

A

P

P

L

L

P

I

G

G

V

V

V

I

G

G

A

S

M

L

M

Q

A

A

L

M

E

E

A

A

I

I

K

K

A

M

V

L

T

A

G

G

A

Y

G

G

Q

K

T

P

L

A

Q

S

G

G

E

K

I

I

L

V

I

M

Y

Y

D

D

G

A

L

M

Y

T

S
x
C

E

Q

S

F

R

R

K

E

I

M

T

K

L

L

S

M

Q

R

R

N

S

P

D

G

C
|
C

P

E

I

V

C
|
C

R14 (= R111) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A141) mutation to A: No effect.
R73 (= R170) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G225) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D227) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (≠ A239) mutation to A: No effect.
C172 (= C269) mutation to A: No zinc bound and no enzyme activity.
C175 (= C272) mutation to A: No zinc bound and no enzyme activity.
C187 (= C286) mutation to A: No effect.
C231 (≠ S330) mutation to A: No effect.
C244 (= C343) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C346) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
41% identity, 65% coverage: 100:346/378 of query aligns to 2:239/240 of 1jwbB

query
sites
1jwbB

K

E

F

L

S

S

E

D

S

Q

E

E

L

M

D

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

R

R

E

G

L

F

G

D

G

F

P

D

G

G

Q

Q

K

E

K

A

L

L

R

K

K

D

A

S

R

R

V

V

L

L

V

I

I

V

G
|
G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

G

T

V

L

I

L

D
|
D

D
x
F

D

D

L

T

V

V

E

S

N

L

A

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

R

S

D

D

Q

A

D

T

I

V

G

G

I

Q

P

P

K
|
K

V

V

F

E

S

S

A

A

Q

R

A

D

A

A

M

L

E

T

A

R

Q

I

N

N

P

P

Y

H

V

I

T

A

V

I

R

T

P

P

Y

V

H

N

R

A

R
x
L

L

L

D

D

A

D

D

A

I

E

A

L

T

A

D

A

L

L

F

I

G

A

D

E

Y

H

D

D

L

L

I

V

L

L

D

D

G
x
C

T
|
T

D
|
D

N
|
N

F

V

E

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

R

A

T

G

A

C

V

F

A

A

L

A

G

K

K

V

P

P

L

L

I

V

S

S

G

G

A

A

L

A

S

I

Q

R

W

M

E

E

G

G

Q

Q

I

I

S

T

L

V

F

F

H

T

P

Y

T

Q

A

D

G

G

G

E

P

P

C
|
C

Y

Y

Q

R

C

C

I

-

F

-

P

-

E

-

A

-

P

-

A

-

G

-

L

L

A

S

P

R

S

L

C

F

S

G

E

E

A

A

G

G

V

V

V

M

G

A

P

P

L

L

P

I

G

G

V

V

V

I

G

G

A

S

M

L

M

Q

A

A

L

M

E

E

A

A

I

I

K

K

A

M

V

L

T

A

G

G

A

Y

G

G

Q

K

T

P

L

A

Q

S

G

G

E

K

I

I

L

V

I

M

Y

Y

D

D

G

A

L

M

Y

T

S

C

E

Q

S

F

R

R

K

E

I

M

T

K

L

L

S

M

Q

R

R

N

S

P

D

G

C
|
C

P

E

I

V

C
|
C

active site: R13 (= R111), D129 (= D227)
binding adenosine monophosphate: G37 (= G135), G39 (= G137), G40 (= G138), D61 (= D159), F62 (≠ D160), K85 (= K183), L108 (≠ R206), C127 (≠ G225), T128 (= T226), D129 (= D227), N130 (= N228), V133 (≠ T231)
binding zinc ion: C171 (= C269), C236 (= C343), C239 (= C346)

1jw9B Structure of the native moeb-moad protein complex (see paper)
41% identity, 65% coverage: 100:346/378 of query aligns to 2:239/240 of 1jw9B

query
sites
1jw9B

K

E

F

L

S

S

E

D

S

Q

E

E

L

M

D

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

R

R

E

G

L

F

G

D

G

F

P

D

G

G

Q

Q

K

E

K

A

L

L

R

K

K

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

G

T

V

L

I

L

D

D

D

F

D

D

L

T

V

V

E

S

N

L

A

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

R

S

D

D

Q

A

D

T

I

V

G

G

I

Q

P

P

K

K

V

V

F

E

S

S

A

A

Q

R

A

D

A

A

M

L

E

T

A

R

Q

I

N

N

P

P

Y

H

V

I

T

A

V

I

R

T

P

P

Y

V

H

N

R

A

R

L

L

L

D

D

A

D

D

A

I

E

A

L

T

A

D

A

L

L

F

I

G

A

D

E

Y

H

D

D

L

L

I

V

L

L

D

D

G

C

T

T

D
|
D

N

N

F

V

E

A

T

V

R

R

Y

N

L

Q

V

L

N

N

R

A

T

G

A

C

V

F

A

A

L

A

G

K

K

V

P

P

L

L

I

V

S

S

G

G

A

A

L

A

S

I

Q

R

W

M

E

E

G

G

Q

Q

I

I

S

T

L

V

F

F

H

T

P

Y

T

Q

A

D

G

G

G

E

P

P

C
|
C

Y

Y

Q

R

C

C

I

-

F

-

P

-

E

-

A

-

P

-

A

-

G

-

L

L

A

S

P

R

S

L

C

F

S

G

E

E

A

A

G

G

V

V

V

M

G

A

P

P

L

L

P

I

G

G

V

V

V

I

G

G

A

S

M

L

M

Q

A

A

L

M

E

E

A

A

I

I

K

K

A

M

V

L

T

A

G

G

A

Y

G

G

Q

K

T

P

L

A

Q

S

G

G

E

K

I

I

L

V

I

M

Y

Y

D

D

G

A

L

M

Y

T

S

C

E

Q

S

F

R

R

K

E

I

M

T

K

L

L

S

M

Q

R

R

N

S

P

D

G

C
|
C

P

E

I

V

C
|
C

active site: R13 (= R111), D129 (= D227)
binding zinc ion: C171 (= C269), C236 (= C343), C239 (= C346)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
45% identity, 65% coverage: 85:328/378 of query aligns to 39:281/460 of O95396

query
sites
O95396

Q

Q

P

P

E

E

E

R

D

L

A

V

E

P

G

V

G

S

A

P

K

L

P

P

R

P

P

K

D

A

K

A

F

L

S

S

E

R

S

D

E

E

L

I

D

L

R

R

Y

Y

A

S

R

R

H

Q

I

L

V

V

L

L

R

P

E

E

L

L

G

G

G

V

P

H

G

G

Q

Q

K

L

K

R

L

L

R

G

K

T

A

A

R

C

V

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

G

G

A

C

P

P

A

L

L

A

Q

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

I

L

G

G

V

L

I

V

D

D

D

Y

D

D

L

V

V

V

E

E

N

M

A

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

I

L

H

H

R

G

D

E

Q

A

D

L

I

A

G

G

I

Q

P

A

K

K

V

A

F

F

S

S

A

A

Q

A

A

A

A

S

M

L

E

R

A

R

Q

L

N

N

P

S

Y

A

V

V

T

E

V

C

R

V

P

P

Y

Y

H
x
T

R
x
Q

R
x
A

L
|
L

D
x
T

A
x
P

D
x
A

I
x
T

A
|
A

T
x
L

D
|
D

L
|
L

F
x
V

G
x
R

D
x
R

Y
|
Y

D
|
D

L
x
V

I
x
V

L
x
A

D
|
D

G
x
C

T
x
S

D
|
D

N
|
N

F
x
V

E
x
P

T
|
T

R
|
R

Y
|
Y

L
|
L

V
|
V

N
|
N

R
x
D

T
x
A

A
x
C

V
|
V

A
x
L

L
x
A

G
|
G

K
x
R

P
|
P

L
|
L

I
x
V

S
|
S

G
x
A

A
x
S

L
x
A

S
x
L

Q
x
R

W
x
F

E
|
E

G
|
G

Q
|
Q

I
|
I

S
x
T

L
x
V

F
x
Y

H
|
H

P

-

T
x
Y

A
x
D

G
|
G

P
|
P

C
|
C

Y
|
Y

Q
x
R

C
|
C

I
|
I

F
|
F

P
|
P

E
x
Q

A
x
P

P
|
P

A
x
P

A
|
A

G
x
E

L
x
T

A
x
V

P
x
T

S
x
N

C
|
C

S

A

E

D

A

G

G

G

V

V

V

L

G

G

P

V

L

V

P

T

G

G

V

V

V

L

G

G

A

C

M

L

M

Q

A

A

L

L

E

E

A

V

I

L

K

K

A

I

V

A

T

A

G

G

A

L

G

G

Q

P

T

S

L

Y

Q

S

G

G

E

S

I

L

L

L

I

L

Y

F

D

D

G

A

L

L

158:238 (vs. 204:285, 44% identical) Interaction with NFS1
C239 (= C286) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
39% identity, 63% coverage: 100:337/378 of query aligns to 2:215/217 of 1jwaB

query
sites
1jwaB

K

E

F

L

S

S

E

D

S

Q

E

E

L

M

D

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

R

R

E

G

L

F

G

D

G

F

P

D

G

G

Q

Q

K

E

K

A

L

L

R

K

K

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

I

L

G

T

V

L

I

L

D
|
D

D
x
F

D

D

L

T

V

V

E

S

N

L

A

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

R

S

D

D

Q

A

D

T

I

V

G

G

I

Q

P

P

K
|
K

V

V

F

E

S

S

A

A

Q

R

A

D

A

A

M

L

E

T

A

R

Q

I

N

N

P

P

Y

H

V

I

T

A

V

I

R

T

P

P

Y

V

H

N

R

A

R
x
L

L

L

D

D

A

D

D

A

I

E

A

L

T

A

D

A

L

L

F

I

G

A

D

E

Y

H

D

D

L

L

I

V

L

L

D

D

G

C

T

T

D
|
D

N
|
N

F

V

E

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

R

A

T

G

A

C

V

F

A

A

L

A

G

K

K

V

P

P

L

L

I

V

S

S

G

G

A

A

L

A

S

I

Q

R

W

M

E

E

G

G

Q

Q

I

I

S

T

L

V

F

F

H

-

P

-

T

-

A

-

G

-

P

-

C

-

Y

-

Q

-

C

-

I

-

F

-

P

-

E

-

A

-

P

-

A

-

G

-

L

-

A

-

P

-

S

-

C

T

S

Y

E

E

A

A

G

G

V

V

V

M

G

A

P

P

L

L

P

I

G

G

V

V

V

I

G

G

A

S

M

L

M

Q

A

A

L

M

E

E

A

A

I

I

K

K

A

M

V

L

T

A

G

G

A

Y

G

G

Q

K

T

P

L

A

Q

S

G

G

E

K

I

I

L

V

I

M

Y

Y

D

D

G

A

L

M

Y

T

S

C

E

Q

S

F

R

R

K

E

I

M

T

K

L

L

active site: R13 (= R111), D129 (= D227)
binding adenosine-5'-triphosphate: G39 (= G137), G40 (= G138), D61 (= D159), F62 (≠ D160), R72 (= R170), K85 (= K183), L108 (≠ R206), D129 (= D227), N130 (= N228), V133 (≠ T231)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 67% coverage: 101:353/378 of query aligns to 1:251/251 of P30138

query
sites
P30138

F

M

S

N

E

D

S

R

E

D

L

F

D

M

R

R

Y

Y

A

S

R

R

H

Q

I

I

V

L

L

L

R

D

E

D

L

I

G

A

G

L

P

D

G

G

Q

Q

K

Q

K

K

L

L

R

L

K

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

G

V

V

L

I

A

D

D

L

D

V

V

E

H

N

L

A

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

R

T

D

T

Q

E

D

D

I

I

G

D

I

R

P

P

K

K

V

S

F

Q

S

V

A

S

Q

Q

A

Q

A

R

M

L

E

T

A

Q

Q

L

N

N

P

P

Y

D

V

I

T

Q

V

L

R

T

P

A

Y

L

H

Q

R

Q

R

R

L

L

D

T

A

G

D

E

I

A

A

L

T

K

D

D

L

A

F

V

G

A

D

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T

T

D

D

N

N

F

M

E

A

T

T

R

R

Y

Q

L

E

V

I

N

N

R

A

T

A

A

C

V

V

A

A

L

L

G

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

L

A

S

V

Q

G

W

F

E

G

G

G

Q

Q

I

L

S

M

L

V

F

L

H

T

P

P

T

P

A

W

G

E

G

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

I

L

F
x
W

P

P

E

D

A

N

P

Q

A

E

A

P

G

-

L

-

A

E

P

R

S

N

C
|
C

S

R

E

T

A

A

G

G

V

V

G

G

P

P

L

V

P

V

G

G

V

V

V

M

G

G

A

T

M

L

M

Q

A

A

L

L

E

E

A

A

I

I

K

K

A

L

V

L

T

S

G

G

A

I

G

-

Q

E

T

T

L

P

Q

A

G

G

E

E

I

L

L

R

I

L

Y

F

D

D

G

G

L

K

Y

S

S

S

E

Q

S

W

R

R

K

S

I

L

T

A

L

L

S

R

Q

R

R

A

S

S

D

G

C
|
C

P

P

I

V

C
|
C

A

G

G

G

-

S

G

N

A

A

G

D

P

P

V

V

C169 (= C269) binding
C172 (= C272) binding
W174 (≠ F274) mutation to A: No adenylation of ThiS.
C184 (= C286) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C343) binding
C243 (= C346) binding

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
42% identity, 55% coverage: 101:309/378 of query aligns to 6:211/423 of 6yubA

query
sites
6yubA

F

L

S

S

E

K

S

E

E

E

L

I

D

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

R

P

E

G

L

M

G

G

G

K

P

E

G

G

Q

Q

K

L

K

R

L

L

R

M

K

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

I

G

G

V

I

I

V

D

D

D

G

D

D

L

S

V

V

E

E

N

T

A

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

R

A

D

T

Q

K

D

R

I

V

G

G

I

M

P

L

K

K

V

V

F

D

S

S

A

L

Q

I

A

T

A

H

M

L

E

I

A

E

Q

I

N

N

P

P

Y

L

V

P

T

V

V

Y

R

V

P

P

Y

Y

H

R

R

F

R

D

L

L

D

T

A

P

D

Q

I

N

A

A

T

A

D

Q

L

I

F

I

G

K

D

P

Y

W

D

D

L

V

I

I

L

L

D

D

G

C

T

T

D

D

N

N

F

P

E

A

T

T

R

R

Y

Y

L

L

V

I

N

S

R

D

T

V

A

C

V

V

A

L

L

L

G

G

K

K

P

P

L

L

I

V

S

S

G

A

A

A

L

S

S

V

Q

Q

W

K

E

S

G

G

Q

Q

I

L

S

I

L

V

F

L

H

N

-

C

-

P

P

P

T

T

A

P

G

Q

G

G

-

V

-

N

-

K

-

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

I

C

F

F

P

-

E

-

A

-

P

-

A

-

G

-

L

-

A

-

P

-

S

-

C

-

S

K

E

K

A

P

G

G

V

I

V

M

G

G

P

P

L

V

P

V

G

G

V

M

G

G

A

V

M

A

M

Q

A

A

L

G

E

E

A

A

I

I

K

K

binding zinc ion: C183 (= C269), C186 (= C272)

Sites not aligning to the query:

binding zinc ion: 266, 269

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
42% identity, 55% coverage: 101:309/378 of query aligns to 7:210/289 of 6yubB

query
sites
6yubB

F

L

S

S

E

K

S

E

E

E

L

I

D

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

R

P

E

G

L

M

G

G

G

K

P

E

G

G

Q

Q

K

L

K

R

L

L

R

M

K

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

I

G

G

V

I

I

V

D

D

D

G

D

D

L

S

V

V

E

E

N

T

A

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

R

A

D

T

Q

K

D

R

I

V

G

G

I

M

P

L

K

K

V

V

F

D

S

S

A

L

Q

I

A

T

A

H

M

L

E

I

A

E

Q

I

N

N

P

P

Y

L

V

P

T

V

V

Y

R

V

P

P

Y

Y

H

R

R

F

R

D

L

L

D

T

A

P

D

Q

I

N

A

A

T

A

D

Q

L

I

F

I

G

K

D

P

Y

W

D

D

L

V

I

I

L

L

D

D

G

C

T

T

D

D

N

N

F

P

E

A

T

T

R

R

Y

Y

L

L

V

I

N

S

R

D

T

V

A

C

V

V

A

L

L

L

G

G

K

K

P

P

L

L

I

V

S

S

G

A

A

A

L

S

S

V

Q

Q

W

K

E

S

G

G

Q

Q

I

L

S

I

L

V

F

L

H

N

-

C

-

P

P

P

T

T

A

P

G

Q

G

G

-

V

-

N

-

K

-

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

I

C

F

F

P

-

E

-

A

-

P

-

A

-

G

-

L

-

A

-

P

-

S

-

C

-

S

-

E

-

A

K

G

G

V

I

V

M

G

G

P

P

L

V

P

V

G

G

V

M

G

G

A

V

M

A

M

Q

A

A

L

G

E

E

A

A

I

I

K

K

binding zinc ion: C184 (= C269), C187 (= C272)

Sites not aligning to the query:

binding zinc ion: 265

1zfnA Structural analysis of escherichia coli thif (see paper)
39% identity, 65% coverage: 101:346/378 of query aligns to 1:243/244 of 1zfnA

query
sites
1zfnA

F

M

S

N

E

D

S

R

E

D

L

F

D

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V

L

L

L

R

D

E

D

L

I

G

A

G

L

P

D

G

G

Q

Q

K

Q

K

K

L

L

R

L

K

D

A

S

R

Q

V

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

G

V

V

L

I

A

D
|
D

D

D

L

D

V

V

E

H

N

L

A

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

R

T

D

T

Q

E

D

D

I

I

G

D

I

R

P

P

K
|
K

V

S

F

Q

S

V

A

S

Q

Q

A

Q

A

R

M

L

E

T

A

Q

Q

L

N

N

P

P

Y

D

V

I

T

Q

V

L

R

T

P

A

Y

L

H

Q

R

Q

R

R

L

L

D

T

A

G

D

E

I

A

A

L

T

K

D

D

L

A

F

V

G

A

D

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T
|
T

D
|
D

N

N

F

M

E

A

T
|
T

R

R

Y

Q

L

E

V

I

N

N

R

A

T

A

A

C

V

V

A

A

L

L

G

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

L

A

S

V

Q

G

W

F

E

G

G

G

Q

Q

I

L

S

M

L

V

F

L

H

T

P

P

T

P

A

W

G

E

G

Q

P

G

C
|
C

Y

Y

Q

R

C
|
C

I

L

F

W

P

P

E

D

A

N

P

Q

A

E

A

P

G

-

L

-

A

E

P

R

S

N

C

C

S

R

E

T

A

A

G

G

V

V

G

G

P

P

L

V

P

V

G

G

V

V

V

M

G

G

A

T

M

L

M

Q

A

A

L

L

E

E

A

A

I

I

K

K

A

L

V

L

T

S

G

G

A

I

G

-

Q

E

T

T

L

P

Q

A

G

G

E

E

I

L

L

R

I

L

Y

F

D

D

G

G

L

K

Y

S

S

S

E

Q

S

W

R

R

K

S

I

L

T

A

L

L

S

R

Q

R

R

A

S

S

D

G

C
|
C

P

P

I

V

C
|
C

active site: R11 (= R111), D127 (= D227)
binding adenosine-5'-triphosphate: I34 (= I134), G35 (= G135), G37 (= G137), G38 (= G138), D59 (= D159), R70 (= R170), Q71 (= Q171), K83 (= K183), T126 (= T226), D127 (= D227), T131 (= T231)
binding zinc ion: C169 (= C269), C172 (= C272), C240 (= C343), C243 (= C346)

1zud3 Structure of this-thif protein complex (see paper)
40% identity, 66% coverage: 101:348/378 of query aligns to 1:240/240 of 1zud3

query
sites
1zud3

F

M

S

N

E

D

S

R

E

D

L

F

D

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V
x
L

L

L

R
x
D

E

D

L

I

G

A

G

L

P

D

G

G

Q

Q

K

Q

K

K

L

L

R

L

K

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

I

L

G

V

V

L

I

A

D

D

L

D

V

V

E

H

N

L

A

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

R

T

D

T

Q

E

D

D

I

I

G

D

I

R

P

P

K

K

V

S

F

Q

S

V

A

S

Q

Q

A

Q

A

R

M

L

E

T

A

Q

Q

L

N

N

P

P

Y

D

V

I

T

Q

V

L

R

T

P

A

Y

L

H

Q

R

Q

R

R

L

L

D

T

A

G

D

E

I

A

A

L

T

K

D

D

L

A

F

V

G

A

D

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T

T

D
|
D

N

N

F

M

E

A

T

T

R

R

Y

Q

L

E

V

I

N

N

R

A

T

A

A

C

V

V

A

A

L

L

G

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

L

A

S

V

Q

G

W

F

E

G

G

G

Q

Q

I

L

S

M

L

V

F

L

H

T

P

P

T

P

A

W

G

E

G

Q

P

G

C
|
C

Y

Y

Q

R

C

C

I

L

F

W

P

P

E

D

A

-

P

-

A

-

G

N

L

Q

A

E

P

P

S

T

C

-

S

-

E

-

A

A

G

G

V

V

G

G

P

P

L

V

P

V

G

G

V

V

V

M

G

G

A

T

M

L

M

Q

A

A

L

L

E

E

A

A

I

I

K

K

A

L

V

L

T

S

G

G

A

I

G

-

Q

E

T

T

L

P

Q

A

G

G

E

E

I

L

L

R

I

L

Y

F

D

D

G

G

L

K

Y

S

S

S

E

Q

S

W

R

R

K

S

I

L

T

A

L

L

S

R

Q

R

R

A

S

S

D

G

C
|
C

P

P

I

V

C
|
C

A

G

G

G

active site: R11 (= R111), D127 (= D227)
binding calcium ion: L14 (≠ V114), D16 (≠ R116)
binding zinc ion: C169 (= C269), C235 (= C343), C238 (= C346)

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 43% coverage: 124:287/378 of query aligns to 21:182/628 of O42939

query
sites
O42939

K

R

K

N

L

F

R

K

K

S

A

A

R

K

V

V

L

L

V

L

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

L

A

M

A

S

G

G

V

V

G

K

T

E

I

V

G

H

V

I

I

I

D

D

D

L

D

D

L

T

V

I

E

D

N

L

A

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

R

R

D

K

Q

K

D

H

I

V

G

K

I

Q

P

P

K

K

V

A

F

I

S

V

A

A

Q

A

A

K

A

T

M

A

E

S

A

S

Q

F

N

N

P

P

Y

N

V

V

T

K

V

L

R

E

P

A

Y

Y

H

H

R

A

R

N

L

I

D

K

A

E

D

D

-

R

I

F

A

N

T

V

D

A

L

W

F

F

G

R

D

Q

Y

F

D

D

L

L

I

V

L

F

D

N

G

A

T

L

D

D

N

N

F

L

E

D

T

A

R

R

Y

R

L

H

V

V

N

N

R

K

T

Q

A

C

V

L

A

L

L

A

G

S

K

V

P

P

L

L

I

I

S

E

G

S

A

G

L

T

S

T

Q

G

W

F

E

L

G

G

Q

Q

I

V

S

Q

-

V

L

I

F

I

H

H

P

-

T

-

A

G

G

K

G

T

P

E

C

C

Y

Y

Q

D

C

C

I

N

F

P

P

K

E

E

A

P

P

P

A

K

A

T

G

-

L

-

A

Y

P

P

S

V

C

C

S

T

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
30% identity, 46% coverage: 115:287/378 of query aligns to 1:175/650 of Q7SXG4

query
sites
Q7SXG4

L

M

R

A

E

E

L

L

G

V

G

G

P

P

G

L

Q

R

K

K

K

Q

L

L

-

A

-

D

-

S

-

L

R

S

K

S

A

C

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

K

T

N

I

I

G

E

V

V

I

I

D

D

D

L

D

D

L

T

V

I

E

D

N

V

A

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

R

Q

D

K

Q

K

D

H

I

V

G

G

I

K

P

S

K

K

V

A

F

Q

S

V

A

A

Q

K

A

E

A

S

M

V

E

L

A

R

Q

F

N

C

P

P

Y

S

V

A

T

N

V

I

R

T

P

A

Y

Y

H

H

R

D

R

S

-

I

L

M

D

N

A

P

D

D

I

Y

A

N

T

V

D

E

L

F

F

F

G

R

D

N

Y

F

D

Q

L

L

I

V

L

M

D

N

G

A

T

L

D

D

N

N

F

R

E

A

T

A

R

R

Y

N

L

H

V

V

N

N

R

R

T

M

A

C

V

L

A

A

L

A

G

D

K

I

P

P

L

L

I

I

S

E

G

S

A

G

L

T

S

A

Q

G

W

Y

E

L

G

G

Q

Q

I

V

S

T

L

V

F

I

H

K

P

-

T

K

A

G

G

Q

G

T

P

E

C

C

Y

Y

Q

E

C

C

I

-

F

-

P

Q

E

P

A

K

P

P

A

T

A

Q

G

K

L

T

A

F

P

P

S

G

C

C

S

T

Sites not aligning to the query:

599 modified: Phosphoserine

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
26% identity, 71% coverage: 80:347/378 of query aligns to 55:335/339 of 3h9jA

query
sites
3h9jA

L

L

R

E

S

T

R

A

A

N

Q

I

P

P

E

E

E

K

D

D

A

F

E

S

G

N

G

C

A

F

K

R

P

F

R

L

P

K

D

E

K

N

F

F

-

F

-

I

-

P

-

G

-

E

-

Y

-

N

S

N

E

S

S

T

E

E

L

N

D

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

R

Y

E

Q

L

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

K

D

K

K

L

L

R

K

K

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G
|
G

L

I

G

G

A

N

P

H

A

V

L

S

Q

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

T

E

I

I

G

I

V

L

I

I

D
|
D

D

N

D
|
D

L

Q

V

I

E

E

N

N

A

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

R

S

D

E

Q

D

D

D

I

V

G

G

I

K

P

N

K
|
K

V

T

F

E

S

V

A

I

Q

K

A

R

A

E

M

L

E

L

A

K

Q

R

N

N

P

S

Y

E

V

I

T

S

V

V

R

S

P

E

Y

I

H

-

R

-

R

A

L

L

D
x
N

A
x
I

D

N

I

D

A

Y

T

T

D

D

L

L

F

H

G

K

-

V

-

P

D

E

Y

A

D

D

L

I

I

W

L

V

D

V

G

S

T
x
A

D
|
D

N
x
H

-

P

F

F

E

N

T

L

R

I

Y

N

L

W

V

V

N

N

R

K

T

Y

A

C

V

V

A

R

L

A

G

N

K

Q

P

P

L

Y

I

I

S

N

G

A

A

G

L

-

S

-

Q

-

W

Y

E

V

G

N

Q

D

I

I

S

A

L

V

F

F

H

G

P

P

T

L

-

Y

-

V

-

P

A

G

G

K

G

T

P

G

C
|
C

Y

Y

Q

E

C

C

I

Q

F

K

P

K

E

E

A

N

-

I

-

D

P

H

A

K

A

I

G

K

L

L

A

I

P

N

S

S

C

R

S

F

E

K

A

P

G

A

V

T

V

F

G

A

P

P

L

V

P

N

G

N

V

V

V

A

G

A

A

A

M

L

M

C

A

A

L

A

E

D

A

V

I

I

K

K

A

F

V

I

T

G

G

K

A

Y

G

S

Q

E

T

P

L

L

Q

S

G

L

E

N

I

K

L
x
R

I

I

Y

-

D

-

G
|
G

L

I

Y

W

S

S

E

D

S

E

R

I

K

K

I

I

T

H

L

S

S

Q

Q

N

R

M

S

G

D

R

C

S

P

P

I

V

C
|
C

A

S

active site: R157 (= R170)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G137), G125 (= G138), D146 (= D159), D148 (= D161), R157 (= R170), K170 (= K183), N193 (≠ D208), I194 (≠ A209), A213 (≠ T226), D214 (= D227), H215 (≠ N228)
binding zinc ion: C257 (= C269), C334 (= C346)
binding : R313 (≠ L323), G315 (= G327)

Sites not aligning to the query:

binding zinc ion: 337

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
26% identity, 71% coverage: 80:347/378 of query aligns to 55:335/338 of 3h5nA

query
sites
3h5nA

L

L

R

E

S

T

R

A

A

N

Q

I

P

P

E

E

E

K

D

D

A

F

E

S

G

N

G

C

A

F

K

R

P

F

R

L

P

K

D

E

K

N

F

F

-

F

-

I

-

P

-

G

-

E

-

Y

-

N

S

N

E

S

S

T

E

E

L

N

D

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

R

Y

E

Q

L

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

K

D

K

K

L

L

R

K

K

N

A

A

R

K

V

V

L

V

V

I

I

L

G

G

A

C

G
|
G

G

G

L

I

G

G

A

N

P

H

A

V

L

S

Q

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

T

E

I

I

G

I

V

L

I

I

D
|
D

D

N

D

D

L

Q

V

I

E

E

N

N

A

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

V

V

I

L

H

F

R

S

D

E

Q

D

D

D

I

V

G

G

I

K

P

N

K
|
K

V

T

F

E

S

V

A

I

Q

K

A

R

A

E

M

L

E

L

A

K

Q

R

N

N

P

S

Y

E

V

I

T

S

V

V

R

S

P

E

Y

I

H

-

R

-

R

A

L

L

D
x
N

A
x
I

D

N

I

D

A

Y

T

T

D

D

L

L

F

H

G

K

-

V

-

P

D

E

Y

A

D

D

L

I

I

W

L

V

D

V

G

S

T
x
A

D
|
D

N
x
H

-

P

F

F

E

N

T

L

R

I

Y

N

L

W

V

V

N

N

R

K

T

Y

A

C

V

V

A

R

L

A

G

N

K

Q

P

P

L

Y

I

I

S

N

G

A

A

G

L

-

S

-

Q

-

W

Y

E

V

G

N

Q

D

I

I

S

A

L

V

F

F

H

G

P

P

T

L

-

Y

-

V

-

P

A

G

G

K

G

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

I

Q

F

K

P

K

E

E

A

N

-

I

-

D

P

H

A

K

A

I

G

K

L

L

A

I

P

N

S

S

C

R

S

F

E

K

A

P

G

A

V

T

V

F

G

A

P

P

L

V

P

N

G

N

V

V

V

A

G

A

A

A

M

L

M

C

A

A

L

A

E

D

A

V

I

I

K

K

A

F

V

I

T

G

G

K

A

Y

G

S

Q

E

T

P

L

L

Q

S

G

L

E

N

I

K

L

R

I

I

Y

-

D

-

G

G

L

I

Y

W

S

S

E

D

S

E

R

I

K

K

I

I

T

H

L

S

S

Q

Q

N

R

M

S

G

D

R

C

S

P

P

I

V

C
|
C

A

S

active site: R157 (= R170)
binding adenosine-5'-triphosphate: G124 (= G137), D146 (= D159), N154 (= N167), R157 (= R170), Q158 (= Q171), K170 (= K183), N193 (≠ D208), I194 (≠ A209), A213 (≠ T226), D214 (= D227), H215 (≠ N228)
binding zinc ion: C257 (= C269), C260 (= C272), C334 (= C346)

Sites not aligning to the query:

binding zinc ion: 337

O65041 NEDD8-activating enzyme E1 catalytic subunit; RUB-activating enzyme; Ubiquitin-activating enzyme E1-like protein; EC 6.2.1.64 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 45% coverage: 120:289/378 of query aligns to 36:218/454 of O65041

query
sites
O65041

G

G

P

P

G

G

-

L

Q

R

K

D

L

I

R

R

K

D

-

Y

A

V

R

R

V

I

L

L

V

V

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

E

A

L

L

L

Q

K

Y

D

L

L

A

A

A

L

A

S

G

G

V

F

G

R

T

N

I

L

G

E

V

V

I

I

D

D

D

M

D

D

L

R

V

I

E

E

N

V

A

T

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

R

R

D

I

Q

E

D

D

I

V

G

G

I

K

P

P

K

K

V

A

F

E

S

V

A

A

Q

A

A

K

A

R

M

V

E

M

A

E

Q

R

N

V

P

S

Y

G

V

V

T

E

V

I

R

V

P

P

Y

H

H

F

R

S

R

R

L

I

D

E

A

-

D

D

I

K

A

E

T

I

D

E

L

F

F

Y

G

N

D

D

Y

F

D

N

L

I

I

I

L

A

D

L

G

G

T

L

D

D

N

S

F

I

E

E

T

A

R

R

Y

K

L

Y

V

I

N

N

R

G

T

V

A

A

V

C

A

G

L

F

-

L

-

E

-

Y

-

N

-

E

-

D

-

T

-

P

-

K

-

R

-

E

-

T

G

I

K

K

P

P

L

M

I

V

S

D

G

G

A

G

L

T

S

E

Q

G

W

F

E

K

G

G

Q

H

I

A

S

R

L

V

F

I

H

L

P

P

T

G

A

V

G

-

G

T

P

P

C

C

Y

F

Q

E

C

C

I

T

F

I

P

Y

E

L

A

F

P

P

A

P

A

Q

G

V

L

K

A

F

P

P

S

L

C
|
C

S

T

E

L

A

A

C215 (= C286) mutation to A: Loss of binding to RUB1.

Query Sequence

>GFF2621 FitnessBrowser__Phaeo:GFF2621
MLSIVVLAALIWWGGRFLGLGHTLRLGLLGVLYIAILAVHLTLPETAPLRGATGGSAAPW
LVLAGMVVLVLAYRHLLRRLRSRAQPEEDAEGGAKPRPDKFSESELDRYARHIVLRELGG
PGQKKLRKARVLVIGAGGLGAPALQYLAAAGVGTIGVIDDDLVENANLQRQVIHRDQDIG
IPKVFSAQAAMEAQNPYVTVRPYHRRLDADIATDLFGDYDLILDGTDNFETRYLVNRTAV
ALGKPLISGALSQWEGQISLFHPTAGGPCYQCIFPEAPAAGLAPSCSEAGVVGPLPGVVG
AMMALEAIKAVTGAGQTLQGEILIYDGLYSESRKITLSQRSDCPICAGGAGPVEETGKKE
TRKETGALHSDAGSSETI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory