SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2631 FitnessBrowser__psRCH2:GFF2631 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9ZCQ4 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Rickettsia prowazekii (strain Madrid E) (see paper)
58% identity, 98% coverage: 3:458/464 of query aligns to 5:460/461 of Q9ZCQ4

query
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Q9ZCQ4

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SGT 98:100 (= SGT 96:98) binding
KVN 324:326 (= KVN 322:324) binding

P05042 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Escherichia coli (strain K12) (see 4 papers)
59% identity, 99% coverage: 2:460/464 of query aligns to 4:462/467 of P05042

query
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P05042

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R126 (≠ S124) binding ; mutation to A: 10-fold decrease of fumarase activity.
K127 (≠ P125) mutation to D: No effect.
H129 (= H127) mutation to N: No effect on fumarase activity and essentially same conformation compared to the wild-type, but appears to dramatically reduce binding of ligands at the B-site.
HPND 129:132 (= HPND 127:130) binding in site B
SSN 139:141 (= SSN 137:139) binding
H188 (= H186) active site, Proton donor/acceptor; mutation to N: 200-fold decrease of fumarase activity.
E315 (= E313) mutation to Q: There is essentially no effect on the affinity values for both S-malate and fumarate. In contrast, the catalytic efficiency values have been lowered by 10-fold in both directions.

1fuqA Fumarase with bound 3-trimethylsilylsuccinic acid (see paper)
59% identity, 98% coverage: 2:457/464 of query aligns to 1:456/456 of 1fuqA

query
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1fuqA

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S

S

I

V

Q

R

L

L

S

A

D

D

G

G

C

M

R

E

N

S

F

F

N

N

T

K

H

H

C

C

I

A

A

V

G

G

L

I

E

E

P

P

D

N

P

R

A

E

R

R

M

I

A

N

E

Q

H

L

L

L

E

N

R

E

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

T

H

H

I

I

G

G

Y

Y

D

D

R

K

A

A

E

E

I

I

A

A

K

K

A

A

Y

H

A

K

Q

E

G

G

T

L

T

T

L

L

R

K

E

A

A

A

S

A

M

L

E

A

L

L

G

G

Y

Y

L

L

S

S

S

E

E

A

E

E

F

F

D

D

Q

S

W

W

V

V

R

R

P

P

E

E

T

Q

M

M

active site: N104 (= N105), T184 (= T185), H185 (= H186), S315 (= S316), K321 (= K322), E328 (= E329)
binding citric acid: T97 (= T98), S136 (= S137), S137 (= S138), N138 (= N139)
binding 3-trimethylsilylsuccinic acid: R123 (≠ S124), H126 (= H127), P127 (= P128), N128 (= N129), D129 (= D130)

1fuoA FumarasE C with bound citrate (see paper)
59% identity, 98% coverage: 2:457/464 of query aligns to 1:456/456 of 1fuoA

query
sites
1fuoA

T

V

R

R

T

S

E

E

T

K

D

D

S

S

L

M

G

G

P

A

V

I

E

D

V

V

P

P

D

A

Q

D

A

K

Y

L

W

W

G

G

A

A

Q

Q

T

T

Q

Q

R

R

S

S

L

L

S

E

N

H

F

F

E

R

I

I

G

S

T

T

E

E

R

K

M

M

P

P

I

T

E

S

V

L

L

I

H

H

A

A

L

L

A

A

L

L

I

T

K

K

K

R

A

A

R

K

V

V

N

N

D

E

R

D

I

L

G

G

D

L

L

L

P

S

P

E

D

E

V

K

A

A

R

S

L

A

I

I

E

R

Q

Q

A

A

D

D

E

E

I

V

L

L

H

A

G

G

Q

Q

H

H

D

D

Q

E

F

F

P

P

L

L

V

A

V

I

W

W

Q

Q

T

T

G

G

S

S

G

G

T
|
T

Q

Q

S

S

N

N

M

M

N

N

V

M

N
|
N

E

E

V

V

I

L

A

A

G

N

R

R

A

A

N

S

E

E

L

L

A

L

G

G

T

V

R

R

G

G

G

M

K

E

S

R

P

K

V

V

H

H

P

P

N

N

D

D

H

D

V

V

N

N

R

K

A

S

Q

Q

S
|
S

N
|
N

D

D

C

V

F

F

P

P

T

T

A

A

M

M

H

H

I

V

A

A

A

L

K

L

A

A

V

L

H

R

A

K

K

Q

L

L

L

I

P

P

A

Q

L

L

A

K

E

T

L

L

S

T

G

Q

G

T

L

L

A

N

E

E

L

K

S

S

V

R

R

A

H

F

G

A

K

D

L

I

I

V

K

K

T

I

G

G

R

R

T
|
T

H
|
H

M

L

M

Q

D

D

A

A

T

T

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

I

S

S

A

G

F

W

V

V

A

A

Q

M

L

L

D

E

I

H

A

N

E

L

R

K

T

H

I

I

R

E

H

Y

T

S

L

L

P

P

A

H

V

V

Y

A

E

E

L

L

A

A

Q

L

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

H

H

P

G

E

F

Y

A

A

E

R

A

R

I

V

A

A

A

D

E

E

L

L

A

A

A

V

L

I

S

T

G

C

L

A

P

P

F

F

V

V

S

T

A

A

P

P

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

T

H

C

E

D

P

A

L

L

V

V

A

Q

L

A

S

H

G

G

A

A

L

L

K

K

T

G

L

L

A

A

V

A

A

S

L

L

M

M

K

K

L

I

A

A

N

N

D

D

L

V

R

R

L

W

L

L

G

A

S

S

G

G

P

P

R

R

G

C

G

G

F

I

A

G

E

E

V

I

R

S

L

I

P

P

A

E

N

N

E

E

P

P

G

G

S
|
S

S

S

I

I

M

M

P

P

G

G

K
|
K

V

V

N

N

P

P

T

T

Q

Q

C

C

E
|
E

A

A

L

L

S

T

M

M

L

L

A

C

C

C

Q

Q

V

V

M

M

G

G

N

N

D

D

M

V

T

A

I

I

S

N

F

M

A

G

S

A

Q

S

G

G

H

N

L

F

Q

E

L

L

N

N

V

V

F

F

K

R

P

P

V

M

I

V

I

I

H

H

N

N

L

F

L

L

Q

Q

S

S

I

V

Q

R

L

L

S

A

D

D

G

G

C

M

R

E

N

S

F

F

N

N

T

K

H

H

C

C

I

A

A

V

G

G

L

I

E

E

P

P

D

N

P

R

A

E

R

R

M

I

A

N

E

Q

H

L

L

L

E

N

R

E

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

T

H

H

I

I

G

G

Y

Y

D

D

R

K

A

A

E

E

I

I

A

A

K

K

A

A

Y

H

A

K

Q

E

G

G

T

L

T

T

L

L

R

K

E

A

A

A

S

A

M

L

E

A

L

L

G

G

Y

Y

L

L

S

S

S

E

E

A

E

E

F

F

D

D

Q

S

W

W

V

V

R

R

P

P

E

E

T

Q

M

M

active site: N104 (= N105), T184 (= T185), H185 (= H186), S315 (= S316), K321 (= K322), E328 (= E329)
binding citric acid: T97 (= T98), S136 (= S137), S137 (= S138), N138 (= N139)

1fupA Fumarase with bound pyromellitic acid (see paper)
59% identity, 98% coverage: 3:457/464 of query aligns to 1:455/455 of 1fupA

query
sites
1fupA

R

R

T

S

E

E

T

K

D

D

S

S

L

M

G

G

P

A

V

I

E

D

V

V

P

P

D

A

Q

D

A

K

Y

L

W

W

G

G

A

A

Q

Q

T

T

Q

Q

R

R

S

S

L

L

S

E

N

H

F

F

E

R

I

I

G

S

T

T

E

E

R

K

M

M

P

P

I

T

E

S

V

L

L

I

H

H

A

A

L

L

A

A

L

L

I

T

K

K

K

R

A

A

R

K

V

V

N

N

D

E

R

D

I

L

G

G

D

L

L

L

P

S

P

E

D

E

V

K

A

A

R

S

L

A

I

I

E

R

Q

Q

A

A

D

D

E

E

I

V

L

L

H

A

G

G

Q

Q

H

H

D

D

Q

E

F

F

P

P

L

L

V

A

V

I

W

W

Q

Q

T

T

G

G

S

S

G

G

T
|
T

Q

Q

S

S

N

N

M

M

N

N

V

M

N
|
N

E

E

V

V

I

L

A

A

G

N

R

R

A

A

N

S

E

E

L

L

A

L

G

G

T

V

R

R

G

G

G

M

K

E

S

R

P

K

V

V

H

H

P

P

N

N

D

D

H

D

V

V

N

N

R

K

A

S

Q

Q

S
|
S

N

N

D

D

C

V

F

F

P

P

T

T

A

A

M

M

H

H

I

V

A

A

A

L

K

L

A

A

V

L

H

R

A

K

K

Q

L

L

L

I

P

P

A

Q

L

L

A

K

E

T

L

L

S

T

G

Q

G

T

L

L

A

N

E

E

L

K

S

S

V

R

R

A

H

F

G

A

K

D

L

I

I

V

K

K

T

I

G

G

R

R

T
|
T

H
|
H

M

L

M

Q

D

D

A

A

T

T

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

I

S

S

A

G

F

W

V

V

A

A

Q

M

L

L

D

E

I

H

A

N

E

L

R

K

T

H

I

I

R

E

H

Y

T

S

L

L

P

P

A

H

V

V

Y

A

E

E

L

L

A

A

Q

L

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

H

H

P

G

E

F

Y

A

A

E

R

A

R

I

V

A

A

A

D

E

E

L

L

A

A

A

V

L

I

S

T

G

C

L

A

P

P

F

F

V

V

S

T

A

A

P

P

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

T

H

C

E

D

P

A

L

L

V

V

A

Q

L

A

S

H

G

G

A

A

L

L

K

K

T

G

L

L

A

A

V

A

A

S

L

L

M

M

K

K

L

I

A

A

N

N

D

D

L

V

R

R

L

W

L

L

G

A

S

S

G

G

P

P

R

R

G

C

G

G

F

I

A

G

E

E

V

I

R

S

L

I

P

P

A

E

N

N

E

E

P

P

G

G

S
|
S

S

S

I

I

M

M

P

P

G

G

K
|
K

V

V

N

N

P

P

T

T

Q

Q

C

C

E
|
E

A

A

L

L

S

T

M

M

L

L

A

C

C

C

Q

Q

V

V

M

M

G

G

N

N

D

D

M

V

T

A

I

I

S

N

F

M

A

G

S

A

Q

S

G

G

H

N

L

F

Q

E

L

L

N

N

V

V

F

F

K

R

P

P

V

M

I

V

I

I

H

H

N

N

L

F

L

L

Q

Q

S

S

I

V

Q

R

L

L

S

A

D

D

G

G

C

M

R

E

N

S

F

F

N

N

T

K

H

H

C

C

I

A

A

V

G

G

L

I

E

E

P

P

D

N

P

R

A

E

R

R

M

I

A

N

E

Q

H

L

L

L

E

N

R

E

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

T

H

H

I

I

G

G

Y

Y

D

D

R

K

A

A

E

E

I

I

A

A

K

K

A

A

Y

H

A

K

Q

E

G

G

T

L

T

T

L

L

R

K

E

A

A

A

S

A

M

L

E

A

L

L

G

G

Y

Y

L

L

S

S

S

E

E

A

E

E

F

F

D

D

Q

S

W

W

V

V

R

R

P

P

E

E

T

Q

M

M

active site: N103 (= N105), T183 (= T185), H184 (= H186), S314 (= S316), K320 (= K322), E327 (= E329)
binding pyromellitic acid: T96 (= T98), S135 (= S137), S136 (= S138)

7lubB Crystal structure of recombinant human fumarase in complex with d-2- amino-3-phosphono-propionic acid (see paper)
58% identity, 98% coverage: 3:458/464 of query aligns to 3:459/462 of 7lubB

query
sites
7lubB

R

R

T

I

E

E

T

Y

D

D

S

T

L

F

G

G

P

E

V

L

E

K

V

V

P

P

D

N

Q

D

A

K

Y

Y

W

Y

G

G

A

A

Q

Q

T

T

Q

V

R

R

S

S

L

T

S

M

N

N

F

F

E

K

I

I

G

G

-

G

-

V

T

T

E

E

R

R

M

M

P

P

I

T

E

P

V

V

L

I

H

K

A

A

L

F

A

G

L

I

I

L

K

K

K

R

A

A

R

E

V

V

N

N

D

Q

R

D

I

Y

G

G

D

-

L

L

P

D

P

P

D

K

V

I

A

A

R

N

L

A

I

I

E

M

Q

K

A

A

D

D

E

E

I

V

L

A

H

E

G

G

Q

K

H

L

D

N

D

D

Q

H

F

F

P

P

L

L

V

V

W

W

Q

Q

T

T

G

G

S

S

G

G

T

T

Q

Q

S

T

N

N

M

M

N

N

V

V

N

N

E

E

V

V

I

I

A

S

G

N

R

R

A

A

N

I

E

E

L

M

A

L

G

G

T

E

R

L

G

G

G

S

K

K

S

I

P

P

V

V

H

H

P

P

N

N

D

D

H

H

V

V

N

N

R

K

A

S

Q

Q

S

S

N

N

D

D

C

T

F

F

P

P

T

T

A

A

M

M

H

H

I

I

A

A

K

I

A

E

V

V

H

H

A

E

K

V

L

L

P

P

A

G

L

L

A

Q

E

K

L

L

S

H

G

D

G

A

L

L

A

D

E

A

L

K

S

S

V

K

R

E

H

F

G

A

K

Q

L

I

I

I

K

K

T

I

G

G

R

R

T
|
T

H
|
H

M

T

M

Q

D

D

A

A

T

V

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

F

S

S

A

G

F

Y

V

V

A

Q

Q

Q

L

V

D

K

I

Y

A

A

E

M

R

T

T

R

I

I

R

K

H

A

T

A

L

M

P

P

A

R

V

I

Y

Y

E

E

L

L

A

A

Q

A

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

R

H

I

G

G

F

F

A

A

E

E

A

K

I

V

A

A

E

K

L

V

A

A

L

L

S

T

G

G

L

L

P

P

F

F

V

V

S

T

A

A

P

P

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

A

H

H

E

D

P

A

L

L

V

V

A

E

L

L

S

S

G

G

A

A

L

M

K

N

T

T

L

T

A

A

V

C

A

S

L

L

M

M

K

K

L

I

A

A

N

N

D

D

L

I

R

R

L

F

L

L

G

G

S

S

G

G

P

P

R

R

G

S

G

G

F

L

A

G

E

E

V

L

R

I

L

L

P

P

A

E

N

N

E

E

P

P

G

G

S
|
S

S

S

I
|
I

M
|
M

P

P

G

G

K
|
K

V

V

N

N

P

P

T

T

Q

Q

C

C

E

E

A

A

L

M

S

T

M

M

L

V

A

A

C

A

Q

Q

V

V

M

M

G

G

N

N

D

H

M

V

T

A

I

V

S

T

F

V

A

G

S

S

Q

N

G

G

H

H

L

F

Q

E

L

L

N

N

V

V

F

F

K

K

P

P

V

M

I

M

I

I

H

K

N

N

L

V

L

L

Q

H

S

S

I

A

Q

R

L

L

S

G

D

D

G

A

C

S

R

V

N

S

F

F

N

T

T

E

H

N

C

C

I

V

A

V

G

G

L

I

E

Q

P

A

D

N

P

T

A

E

R

R

M

I

A

N

E

K

H

L

L

M

E

N

R

E

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

P

H

H

I

I

G

G

Y

Y

D

D

R

K

A

A

E

K

I

I

A

A

K

K

K

T

A

A

Y

H

A

K

Q

N

G

G

T

S

T

T

L

L

R

K

E

E

A

T

S

A

M

I

E

E

L

L

G

G

Y

Y

L

L

S

T

S

A

E

E

E

Q

F

F

D

D

Q

E

W

W

V

V

R

K

P

P

E

K

T

D

M

M

L

L

binding d-2-amino-3-phosphono-propionic acid: T186 (= T185), H187 (= H186), S317 (= S316), I319 (= I318), M320 (= M319), K323 (= K322)

P07954 Fumarate hydratase, mitochondrial; Fumarase; HsFH; EC 4.2.1.2 from Homo sapiens (Human) (see 4 papers)
58% identity, 98% coverage: 3:458/464 of query aligns to 51:507/510 of P07954

query
sites
P07954

R

R

T

I

E

E

T

Y

D

D

S

T

L

F

G

G

P

E

V

L

E

K

V

V

P

P

D

N

Q

D

A

K

Y

Y

W

Y

G

G

A

A

Q

Q

T

T

Q

V

R

R

S

S

L

T

S

M

N

N

F

F

E

K

I

I

G

G

-

G

-

V

T

T

E

E

R

R

M

M

P

P

I

T

E

P

V

V

L

I

H

K

A

A

L

F

A

G

L

I

I

L

K

K

K

R

A

A

R

E

V

V

N

N

D

Q

R

D

I

Y

G

G

D

-

L

L

P

D

P

P

D

K

V

I

A

A

R

N

L

A

I

I

E

M

Q

K

A

A

D

D

E

E

I

V

L

A

H

E

G

G

Q

K

H

L

D

N

D

D

Q

H

F

F

P

P

L

L

V

V

W

W

Q

Q

T

T

G

G

S

S

G

G

T
|
T

Q

Q

S

T

N

N

M

M

N

N

V

V

N

N

E

E

V

V

I

I

A

S

G

N

R

R

A

A

N

I

E

E

L

M

A

L

G

G

T

E

R

L

G

G

G

S

K

K

S

I

P

P

V

V

H

H

P

P

N

N

D

D

H

H

V

V

N

N

R

K

A

S

Q

Q

S

S

S
|
S

N

N

D

D

C

T

F

F

P

P

T

T

A

A

M

M

H

H

I

I

A

A

K

I

A

E

V

V

H

H

A

E

K

V

L

L

P

P

A

G

L

L

A

Q

E

K

L

L

S

H

G

D

G

A

L

L

A

D

E

A

L

K

S

S

V

K

R

E

H

F

G

A

K

Q

L

I

I

I

K
|
K

T

I

G

G

R
|
R

T

T

H

H

M
x
T

M

Q

D

D

A

A

T

V

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

F

S

S

A

G

F

Y

V

V

A

Q

Q

Q

L

V

D

K

I

Y

A

A

E

M

R

T

T

R

I

I

R

K

H

A

T

A

L

M

P

P

A

R

V

I

Y

Y

E

E

L

L

A

A

Q

A

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

R

H

I

G

G

F

F

A

A

E

E

A

K

I

V

A

A

E

K

L

V

A

A

L

L

S

T

G

G

L

L

P

P

F

F

V

V

S

T

A

A

P

P

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

A

H

H

E

D

P

A

L

L

V

V

A

E

L

L

S

S

G

G

A

A

L

M

K

N

T

T

L

T

A

A

V

C

A

S

L

L

M

M

K

K

L

I

A

A

N

N

D

D

L

I

R

R

L

F

L

L

G

G

S

S

G

G

P

P

R

R

G

S

G

G

F

L

A

G

E

E

V

L

R

I

L

L

P

P

A

E

N

N

E

E

P

P

G

G

S

S

I

I

M

M

P

P

G

G

K

K

V

V

N

N

P

P

T

T

Q

Q

C

C

E

E

A

A

L

M

S

T

M

M

L

V

A

A

C

A

Q

Q

V

V

M

M

G

G

N

N

D

H

M

V

T

A

I

V

S

T

F

V

A

G

S

S

Q

N

G

G

H

H

L

F

Q

E

L

L

N

N

V

V

F

F

K

K

P

P

V

M

I

M

I

I

H

K

N

N

L

V

L

L

Q

H

S

S

I

A

Q

R

L

L

S

G

D

D

G

A

C

S

R

V

N

S

F

F

N

T

T

E

H

N

C

C

I

V

A

V

G

G

L

I

E

Q

P

A

D

N

P

T

A

E

R

R

M

I

A

N

E

K

H

L

L

M

E

N

R

E

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

P

H

H

I

I

G

G

Y

Y

D

D

R

K

A

A

E

K

I

I

A

A

K

K

K

T

A

A

Y

H

A

K

Q

N

G

G

T

S

T

T

L

L

R

K

E

E

A

T

S

A

M

I

E

E

L

L

G

G

Y

Y

L

L

S

T

S

A

E

E

E

Q

F

F

D

D

Q

E

W

W

V

V

R

K

P

P

E

K

T

D

M

M

L

L

T147 (= T98) mutation to A: Does not affect phosphorylation by PRKDC.
S187 (= S138) mutation to A: Does not affect phosphorylation by PRKDC.
K230 (= K181) to R: in FMRD and HLRCC; dbSNP:rs752232718
R233 (= R184) to H: in HLRCC; catalytically inactive mutant; abolished ability to promote DNA repair; dbSNP:rs121913123
T236 (≠ M187) modified: Phosphothreonine; by PRKDC; mutation to A: Abolished interaction with H2AZ1 and localization to chromatin in response to DNA damage.; mutation to D: Phosphomimetic mutant; promotes interaction with H2AZ1, leading to increased localization to chromatin in response to DNA damage.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
1:43 modified: Variant sequence, Missing (in isoform Cytoplasmic)
46 S→A: Does not affect phosphorylation by PRKDC.

7c18B Crystal structure of fumarasec from mannheimia succiniciproducens in complex with fumarate
56% identity, 99% coverage: 3:460/464 of query aligns to 4:462/464 of 7c18B

query
sites
7c18B

R

R

T

I

E

E

T

K

D

D

S

T

L

M

G

G

P

E

V

V

E

Q

V

V

P

P

D

A

Q

D

A

K

Y

Y

W

W

G

A

A

A

Q

Q

T

T

Q

E

R

R

S

S

L

R

S

N

N

N

F

F

E

K

I

I

G

G

-

P

T

A

E

A

R

S

M

M

P

P

I

H

E

E

V

I

L

I

H

E

A

A

L

F

A

G

L

Y

I

L

K

K

A

A

R

F

V

A

N

N

D

T

R

D

I

L

G

G

D

V

L

L

P

P

P

A

D

E

V

K

A

R

R

D

L

L

I

I

E

G

Q

Q

A

A

A

C

D

D

E

E

I

I

L

L

H

A

G

R

Q

K

H

L

D

D

Q

Q

F

F

P

P

L

L

V

V

V

I

W

W

Q

Q

T

T

G

G

S

S

G

G

T
|
T

Q

Q

S

S

N

N

M

M

N

N

V

L

N

N

E

E

V

V

I

I

A

A

G

N

R

R

A

A

N

H

E

V

L

I

A

N

G

G

T

K

R

L

G

G

G

E

K

K

S

S

P

I

V

I

H

H

P

P

N

N

D

D

H

D

V

V

N

N

R

K

A

S

Q

Q

S
|
S

N
|
N

D

D

C

T

F

Y

P

P

T

T

A

A

M

M

H

H

I

I

A

A

A

Y

K

K

A

K

V

V

H

V

A

E

K

A

L

T

L

I

P

P

A

A

L

V

A

E

E

R

L

L

S

Q

G

K

G

T

L

L

A

A

E

A

L

K

S

A

V

A

R

E

H

F

G

K

K

D

L

V

I

V

K

K

T

I

G

G

R

R

T
|
T

H
|
H

M

L

M

M

D

D

A

A

T

T

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

F

S

S

A

G

F

Y

V

A

A

A

Q

Q

L

L

D

S

I

F

A

G

E

L

R

T

T

A

I

I

R

K

H

N

T

T

L

L

P

P

A

H

V

L

Y

R

E

Q

L

L

A

A

Q

L

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

P

H

K

G

G

F

Y

A

D

E

V

A

K

I

V

A

A

A

E

E

Y

L

I

A

A

A

K

L

F

S

T

G

G

L

L

P

P

F

F

V

I

S

T

A

A

P

E

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

T

H

H

E

D

P

A

L

I

V

V

A

E

L

T

S

H

G

G

A

A

L

L

K

K

T

Q

L

V

A

A

V

M

A

S

L

L

M

F

K

K

L

I

A

A

N

N

D

D

L

I

R

R

L

L

G

A

S

S

G

G

P

P

R

R

G

S

G

G

F

I

A

G

E

E

V

I

R

L

L

I

P

P

A

E

N

N

E

E

P

P

G

G

S
x
C

S
|
S

I

I

M
|
M

P

P

G

G

K
|
K

V

V

N
|
N

P

P

T

T

Q

Q

C

C

E

E

A

A

L

M

S

T

M

M

L

V

A

A

C

A

Q

Q

V

V

M

L

G

G

N

N

D

D

M

T

T

T

I

I

S

S

F

F

A

A

A

G

S

S

Q

Q

G

G

H

H

L

F

Q

E

L

L

N

N

V

V

F

F

K

K

P

P

V

V

I

M

I

A

H

A

N

N

L

F

L

L

Q

Q

S

S

I

A

Q

Q

L

L

L

I

S

A

D

D

G

V

C

C

R

I

N

S

F

F

N

D

T

E

H

H

C

C

I

A

A

T

G

G

L

I

E

Q

P

P

D

N

P

T

A

P

R

R

M

I

A

Q

E

H

H

L

L

L

E

D

R

S

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

T

H

H

I

I

G

G

Y

Y

D

E

R

N

A

A

E

K

I

I

A

A

K

K

K

T

A

A

Y

H

A

K

Q

N

G

G

T

T

L

L

R

R

E

E

A

E

S

A

M

I

E

N

L

L

G

G

Y

L

L

V

S

S

S

A

E

E

E

D

F

F

D

D

Q

K

W

W

V

V

R

V

P

P

E

A

T

D

M

M

L

V

E

G

S

S

binding fumaric acid: T100 (= T98), S139 (= S137), S140 (= S138), N141 (= N139), T187 (= T185), H188 (= H186), C318 (≠ S316), S319 (= S317), M321 (= M319), K324 (= K322), N326 (= N324)

P08417 Fumarate hydratase, mitochondrial; Fumarase; EC 4.2.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
55% identity, 98% coverage: 3:458/464 of query aligns to 29:485/488 of P08417

query
sites
P08417

R
|
R

T
|
T

E
|
E

T
|
T

D
|
D

S
x
A

L
x
F

G
|
G

P
x
E

V
x
I

E
x
H

V
|
V

P
|
P

D
x
A

Q
x
D

A
x
K

Y

Y

W

W

G

G

A

A

Q

Q

T

T

Q

Q

R

R

S

S

L

F

S

Q

N

N

F

F

E

K

I

I

G

G

-

G

-

A

T

R

E

E

R

R

M

M

P

P

I

L

E

P

V

L

L

V

H

H

A

A

L

F

A

G

L

V

I

L

K

K

A

S

A

A

R

I

V

V

N

N

D

E

R

S

I

L

G

G

D

G

L

L

P

D

P

P

D

K

V

I

A

S

R

K

L

A

I

I

E

Q

Q

Q

A

A

D

D

E

E

I

V

L

A

H

S

G

G

Q

K

H

L

D

D

Q

H

F

F

P

P

L

L

V

V

W

F

Q

Q

T

T

G

G

S

S

G

G

T

T

Q

Q

S

S

N

N

M

M

N

N

V

A

N

N

E

E

V

V

I

I

A

S

G

N

R

R

A

A

N

I

E

E

L

I

A

L

G

G

T

K

R

I

G

G

G

S

K

K

S

Q

P

-

V

V

H
|
H

P

P

N

N

D

N

H

H

V

C

N

N

R

Q

A

S

Q

Q

S

S

N

N

D

D

C

T

F

F

P

P

T

T

A

V

M

M

H

H

I

I

A

A

A

S

K

L

A

Q

V

I

H

Q

A

N

K

E

L

L

L

I

P

P

A

E

L

L

A

T

E

N

L

L

S

K

G

N

G

A

L

L

A

E

E

A

L

K

S

S

V

K

R

E

H

F

G

D

K

H

L

I

I

V

K

K

T

I

G

G

R

R

T

T

H

H

M

L

M

Q

D

D

A

A

T

T

P

P

I

L

T

T

F

L

G

G

Q

Q

E

E

M

F

S

S

A

G

F

Y

V

V

A

Q

Q

Q

L

V

D

E

I

N

A

G

E

I

R

Q

T

R

I

V

R

A

H

H

T

S

L

L

P

K

A

T

V

L

Y

S

E

F

L

L

A

A

Q

Q

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

T

P

K

H

P

G

G

F

F

A

D

E

V

A

K

I

I

A

A

A

E

E

Q

L

I

A

S

A

K

L

E

S

T

G

G

L

L

P

K

F

F

V

Q

S

T

A

A

P

P

N

N

K

K

F

F

A

E

A

A

L

L

A

A

G

A

H

H

E

D

P

A

L

I

V

V

A

E

L

C

S

S

G

G

A

A

L

L

K

N

T

T

L

L

A

A

V

C

A

S

L

L

M

F

K

K

L

I

A

A

N

Q

D

D

L

I

R

R

L

Y

L

L

G

G

S

S

G

G

P

P

R

R

G

C

G

G

F

Y

A

H

E

E

V

L

R

M

L

L

P

P

A

E

N

N

E

E

P

P

G

G

S

S

I

I

M

M

P

P

G

G

K

K

V

V

N

N

P

P

T

T

Q

Q

C

N

E

E

A

A

L

L

S

T

M

Q

L

V

A

C

C

V

Q

Q

V

V

M

M

G

G

N

N

D

N

M

A

T

A

I

I

S

T

F

F

A

A

A

G

S

S

Q

Q

G

G

H

Q

L

F

Q

E

L

L

N

N

V

V

F

F

K

K

P

P

V

V

I

M

I

I

H

A

N

N

L

L

Q

N

S

S

I

I

Q

R

L

L

L

I

S

T

D

D

G

A

C

A

R

Y

N

S

F

F

N

R

T

V

H

H

C

C

I

V

A

E

G

G

L

I

E

K

P

A

D

N

P

E

A

P

R

R

M

I

A

H

E

E

H

L

L

L

E

T

R

K

G

S

L

L

M

M

L

L

V

V

T

T

A

A

L

L

N

N

P

P

H

K

I

I

G

G

Y

Y

D

D

R

A

A

A

A

S

E

K

I

V

A

A

K

K

K

N

A

A

Y

H

A

K

Q

K

G

G

T

I

T

T

L

L

R

K

E

E

A

S

S

A

M

L

E

E

L

L

G

G

Y

V

L

L

S

T

S

E

E

K

E

E

F

F

D

D

Q

E

W

W

V

V

R

V

P

P

E

E

T

H

M

M

L

L

29:44 (vs. 3:18, 50% identical) mutation Missing: Does not affect subcellular location.
H154 (= H127) mutation to R: Abolished fumarate hydratase activity and ability to participate in DNA repair.

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion
24 M→S: Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.; mutation M->V,I: Abolishes processing by the mitochondrial processing peptidase. Mainly localizes in the cytosol, with a small fraction in the mitochondrion. Reduced fumarate hydratase activity.
24:25 MN→SF: Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.

P9WN93 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
47% identity, 97% coverage: 3:450/464 of query aligns to 11:456/474 of P9WN93

query
sites
P9WN93

R

R

T

I

E

E

T

H

D

D

S

T

L

M

G

G

P

E

V

V

E

R

V

V

P

P

D

A

Q

K

A

A

Y

L

W

W

G

R

A

A

Q

Q

T

T

Q

Q

R

R

S

A

L

V

S

E

N

N

F

F

E

P

I

I

G

S

T

G

E

R

R

G

M

L

P

E

I

R

E

T

V

Q

L

I

H

R

A

A

L

L

A

G

L

L

I

L

K

K

K

G

A

A

A

C

A

A

R

Q

V

V

N

N

D

S

R

D

I

L

G

G

D

L

L

L

P

A

P

P

D

E

V

K

A

A

R

D

L

A

I

I

E

I

Q

A

A

A

D

A

E

E

I

I

L

A

H

D

G

G

Q

Q

H

H

D

D

Q

Q

F

F

P

P

L

I

V

D

V

V

W

F

Q

Q

T

T

G

G

S
|
S

G
|
G

T
|
T

Q

S

S

S

N

N

M

M

N

N

V

T

N

N

E

E

V

V

I

I

A

A

G

S

R

I

A

A

N

-

E

-

L

-

A

-

G

-

T

A

R

K

G

G

K

V

S

T

P

-

V

L

H

H

P

P

N

N

D

D

H

D

V

V

N

N

R

M

A

S

Q

Q

S
|
S

N
|
N

D

D

C

T

F

F

P

P

T

T

A

A

M

T

H

H

I

I

A

A

A

T

K

E

A

A

V

A

H

V

A

A

K

H

L

L

L

I

P

P

A

A

L

L

A

Q

E

Q

L

L

S

H

G

D

G

A

L

L

A

A

E

A

L

K

S

A

V

L

R

D

H

W

G

H

K

T

L

V

I

V

K

K

T

S

G

G

R

R

T
|
T

H

H

M

L

M

M

D

D

A

A

T

V

P

P

I

V

T

T

F

L

G

G

Q

Q

E

E

M

F

S

S

A

G

F

Y

V

A

A

R

Q

Q

L

I

D

E

I

A

A

G

E

I

R

E

T

R

I

V

R

R

H

A

T

C

L

L

P

P

A

R

V

L

Y

G

E

E

L

L

A

A

Q

I

G

G

T

T

A

A

V

V

G

G

T

T

G

G

L

L

N

N

S

A

P

P

H

D

G

D

F

F

A

G

E

V

A

R

I

V

A

V

A

A

E

V

L

L

A

V

A

A

L

Q

S

T

G

G

L

L

P

S

-

E

F

L

V

R

S

T

A

A

P

A

N

N

K

S

F

F

A

E

A

A

L

Q

A

A

G

A

H

R

E

D

P

G

L

L

V

V

A

E

L

A

S

S

G

G

A

A

L

L

K

R

T

T

L

I

A

A

V

V

A

S

L

L

M

T

K

K

L

I

A

A

N

N

D

D

L

I

R

R

L

W

L

M

G

G

S

S

G

G

P

P

R

L

G

T

G

G

F

L

A

A

E

E

V

I

R

Q

L

L

P

P

A

D

N

L

E

Q

P

P

G

G

S
|
S

I

I

M

M

P

P

G

G

K
|
K

V
|
V

N
|
N

P

P

T

V

Q

L

C

P

E

E

A

A

L

V

S

T

M

Q

L

V

A

A

C

A

Q

Q

V

V

M

I

G

G

N

N

D

D

M

A

T

A

I

I

S

A

F

W

A

G

S

A

Q

N

G

G

H

A

L

F

Q

E

L

L

N

N

V

V

F

Y

K

I

P

P

V

M

I

M

I

A

H

R

N

N

L

I

L

L

Q

E

S

S

I

F

Q

K

L

L

S

T

D

N

G

V

C

S

R

R

N

L

F

F

N

A

T

Q

H

R

C

C

I

I

A

A

G

G

L

L

E

T

P

A

D

N

P

V

A

E

R

H

M

L

A

R

E

R

H

L

L

A

E

E

R

S

G

S

L

P

M

S

L

I

V

V

T

T

A

P

L

L

N

N

P

S

H

A

I

I

G

G

Y

Y

D

E

R

E

A

A

E

A

I

V

A

A

K

K

K

Q

A

A

Y

L

A

K

Q

E

G

R

T

K

T

T

L

I

R

R

E

Q

A

T

S

V

M

I

E

D

L

R

G

G

Y

L

-

I

-

G

-

D

-

R

L

L

S

S

S

I

E

E

E

D

F

L

D

D

Q

R

SGT 104:106 (= SGT 96:98) binding
SSN 138:140 (= SSN 137:139) binding
T186 (= T185) binding
S318 (= S316) active site; mutation S->A,C: Absence of fumarase activity.
S319 (= S317) binding
KVN 324:326 (= KVN 322:324) binding

4adlA Crystal structures of rv1098c in complex with malate (see paper)
47% identity, 97% coverage: 3:450/464 of query aligns to 3:448/459 of 4adlA

query
sites
4adlA

R

R

T

I

E

E

T

H

D

D

S

T

L

M

G

G

P

E

V

V

E

R

V

V

P

P

D

A

Q

K

A

A

Y

L

W

W

G

R

A

A

Q

Q

T

T