SitesBLAST
Comparing GFF2640 FitnessBrowser__Phaeo:GFF2640 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 13 hits to proteins with known functional sites (download)
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
68% identity, 97% coverage: 11:433/436 of query aligns to 4:427/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K72), S90 (= S97), S91 (= S98), G92 (= G99), T93 (= T100), T94 (= T101), F138 (= F145), A211 (= A218), E212 (= E219), P213 (= P220), D232 (= D239), I233 (= I240), Y234 (= Y241), G235 (= G242), L236 (= L243), S237 (= S244), D302 (= D309), I320 (= I327), R323 (= R330), K419 (= K425)
- binding magnesium ion: V200 (≠ R207), S202 (≠ C209), L204 (= L211), M226 (≠ F233), G227 (≠ D234), Q347 (≠ T354), L350 (= L357)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
67% identity, 97% coverage: 11:433/436 of query aligns to 4:425/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y140), F136 (= F145), G138 (= G147), G208 (= G217), A209 (= A218), E210 (= E219), P211 (= P220), I231 (= I240), Y232 (= Y241), G233 (= G242), L234 (= L243), S235 (= S244), P240 (= P249), D300 (= D309), R321 (= R330), K417 (= K425)
- binding magnesium ion: V198 (≠ R207), S200 (≠ C209), Q345 (≠ T354), L348 (= L357)
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
67% identity, 97% coverage: 11:433/436 of query aligns to 6:432/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y140), F140 (= F145), A213 (= A218), E214 (= E219), P215 (= P220), I235 (= I240), G237 (= G242), L238 (= L243), S239 (= S244), P244 (= P249), D304 (= D309), R325 (= R330), I331 (= I336), N336 (= N341)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
67% identity, 97% coverage: 11:433/436 of query aligns to 6:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y140), F140 (= F145), G212 (= G217), A213 (= A218), E214 (= E219), P215 (= P220), I235 (= I240), G237 (= G242), L238 (= L243), S239 (= S244), P244 (= P249), D304 (= D309), R325 (= R330), I331 (= I336), N336 (= N341)
- binding magnesium ion: S204 (≠ C209), V228 (≠ F233)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
46% identity, 97% coverage: 12:433/436 of query aligns to 9:433/435 of 4r1mA
- binding adenosine monophosphate: A215 (= A218), E216 (= E219), P217 (= P220), N236 (≠ D239), S237 (≠ I240), F238 (≠ Y241), G239 (= G242), M240 (≠ L243), T241 (≠ S244), D305 (= D309), R329 (= R330), I335 (= I336), N340 (= N341)
- binding zinc ion: C252 (= C255), H259 (≠ V263), C314 (≠ A318), C316 (vs. gap)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
46% identity, 97% coverage: 12:433/436 of query aligns to 9:431/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (= A218), E216 (= E219), P217 (= P220), S237 (≠ I240), F238 (≠ Y241), G239 (= G242), M240 (≠ L243), T241 (≠ S244), D305 (= D309), R329 (= R330), N340 (= N341)
- binding adenosine monophosphate: A215 (= A218), E216 (= E219), P217 (= P220), S237 (≠ I240), F238 (≠ Y241), G239 (= G242), M240 (≠ L243), T241 (≠ S244), D305 (= D309), R329 (= R330), N340 (= N341)
- binding coenzyme a: S136 (≠ A139), A164 (≠ G167), G165 (= G168), N166 (≠ Q169), S167 (≠ T170), I185 (≠ T188), Y188 (= Y191), K337 (≠ R338), T408 (≠ S408)
- binding zinc ion: C252 (= C255), H259 (≠ V263), C314 (≠ A318), C316 (vs. gap)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
30% identity, 93% coverage: 27:433/436 of query aligns to 42:464/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G217), G242 (≠ A218), E243 (= E219), P244 (= P220), G267 (≠ Y241), S268 (≠ G242), M269 (≠ L243), A270 (≠ S244), D335 (= D309), I357 (= I327), N371 (= N341)
- binding adenosine monophosphate: G242 (≠ A218), E243 (= E219), P244 (= P220), C266 (≠ I240), G267 (≠ Y241), S268 (≠ G242), A270 (≠ S244), E271 (= E245), D335 (= D309), N371 (= N341)
- binding coenzyme a: Y166 (≠ F145), A188 (≠ G167), G189 (= G168), P191 (vs. gap), S194 (≠ T170), Y210 (≠ M186), G211 (≠ V187), T212 (= T188), Y215 (= Y191), H218 (≠ N194), R368 (= R338), G369 (= G339), M401 (= M371), V439 (= V407), R440 (≠ S408)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
30% identity, 93% coverage: 27:433/436 of query aligns to 42:461/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F145), S237 (≠ G217), G263 (≠ Y241), S264 (≠ G242), M265 (≠ L243), A266 (≠ S244), F271 (≠ G250)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F145), G164 (= G147), S237 (≠ G217), G238 (≠ A218), E239 (= E219), P240 (= P220), C262 (≠ I240), G263 (≠ Y241), S264 (≠ G242), A266 (≠ S244), F271 (≠ G250), D331 (= D309), I353 (= I327), R356 (= R330), K453 (= K425)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
30% identity, 93% coverage: 27:433/436 of query aligns to 42:461/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F145), G164 (= G147), S237 (≠ G217), G238 (≠ A218), E239 (= E219), P240 (= P220), C262 (≠ I240), G263 (≠ Y241), S264 (≠ G242), A266 (≠ S244), F271 (≠ G250), D331 (= D309), I353 (= I327), R356 (= R330), K453 (= K425)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F145), G164 (= G147), S237 (≠ G217), G263 (≠ Y241), S264 (≠ G242), A266 (≠ S244), F271 (≠ G250)
6siyA Paak family amp-ligase with amp and substrate (see paper)
30% identity, 67% coverage: 17:309/436 of query aligns to 12:296/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ Y140), P126 (≠ G141), T132 (≠ G147), L135 (≠ G150), R153 (≠ G168), N177 (≠ T188), A209 (= A218), E232 (≠ I240), G234 (= G242), S235 (≠ L243)
- binding adenosine monophosphate: S85 (= S98), A209 (= A218), E210 (= E219), P211 (= P220), E232 (≠ I240), Y233 (= Y241), G234 (= G242), S235 (≠ L243), T236 (≠ S244), D296 (= D309)
- binding magnesium ion: R75 (= R88), E76 (= E89), L78 (≠ I91), P117 (≠ K132), G144 (= G159), A145 (= A160), T146 (= T161)
Sites not aligning to the query:
6sixB Paak family amp-ligase with anp (see paper)
30% identity, 67% coverage: 17:309/436 of query aligns to 16:300/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (= S97), S89 (= S98), A213 (= A218), E214 (= E219), P215 (= P220), E236 (≠ I240), Y237 (= Y241), G238 (= G242), S239 (≠ L243), T240 (≠ S244), E241 (= E245), D300 (= D309)
- binding magnesium ion: R79 (= R88), E80 (= E89), P121 (≠ K132), T150 (= T161)
- binding zinc ion: C249 (= C255), H255 (≠ G261)
Sites not aligning to the query:
6siwA Paak family amp-ligase with amp (see paper)
30% identity, 67% coverage: 17:309/436 of query aligns to 11:295/432 of 6siwA
- binding adenosine monophosphate: S84 (= S98), A208 (= A218), E209 (= E219), P210 (= P220), E231 (≠ I240), Y232 (= Y241), G233 (= G242), S234 (≠ L243), T235 (≠ S244), D295 (= D309)
- binding magnesium ion: E75 (= E89), L77 (≠ I91), S83 (= S97), P116 (≠ K132), G143 (= G159), T145 (= T161), E236 (= E245)
- binding zinc ion: C244 (= C255), H250 (≠ G261)
Sites not aligning to the query:
1t5dX 4-chlorobenzoyl-coa ligase/synthetase bound to 4-chlorobenzoate (see paper)
28% identity, 42% coverage: 176:359/436 of query aligns to 237:423/502 of 1t5dX
Sites not aligning to the query:
Query Sequence
>GFF2640 FitnessBrowser__Phaeo:GFF2640
MEDLSPKKGELDPIEIASIDEIRSLQLDRLKWSLRHAYDNVAMYKQRFDEAGVHPDDLQQ
LSDLAKFPFTYKNDLRDNYPFGLFAVPREQIMRLHASSGTTGKPTVVGYTANDISNWADL
VARSLRASGLRKGDMVHNAYGYGLFTGGLGAHYGIERLGATVVPMSGGQTEKQVGLITDF
KPDGIMVTPSYMLNILEQYHKVGMDPRECSLKVGVFGAEPWTDAMRREVEQAFDMHAVDI
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPQTGEVLPDGEMGELVFTTLTKEGL
PMVRYRTRDLTRLLPGTARSMRRMEKITGRSDDMIILRGVNVFPSQVEEQLLATGGLAPY
YQIELYKSGRMDAMRVFVEANPDATDELSKTAAARMLTKRIKDMVGVSTEIIVGEPGSVE
RSQGKAKRVVDNRSKE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory