SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2646 FitnessBrowser__Phaeo:GFF2646 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 86% coverage: 15:239/261 of query aligns to 22:247/265 of P07821

query
sites
P07821

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K50 (= K46) mutation to Q: Lack of activity.
D172 (= D168) mutation to E: Lack of activity.
E173 (= E169) mutation to A: Lack of activity.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
37% identity, 80% coverage: 23:230/261 of query aligns to 21:230/280 of 5x40A

query
sites
5x40A

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binding phosphomethylphosphonic acid adenylate ester: N40 (= N42), G41 (= G43), G43 (= G45), K44 (= K46), S45 (= S47), T46 (≠ S48), Q88 (= Q88), H139 (≠ G142), M140 (≠ T143), L141 (= L144), S142 (= S145), G144 (= G147), Q145 (≠ E148), Q166 (≠ E169), H198 (= H198)
binding magnesium ion: S45 (= S47), Q88 (= Q88)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14, 18, 20

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
28% identity, 83% coverage: 22:237/261 of query aligns to 17:233/240 of 6mjpA

query
sites
6mjpA

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binding calcium ion: E111 (≠ P116), D115 (= D120)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
31% identity, 79% coverage: 23:227/261 of query aligns to 22:216/353 of 1vciA

query
sites
1vciA

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binding adenosine-5'-triphosphate: S41 (≠ N42), G42 (= G43), G44 (= G45), K45 (= K46), T46 (≠ S47), T47 (≠ S48)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

Q9LVM1 ABC transporter B family member 25, mitochondrial; ABC transporter ABCB.25; AtABCB25; ABC transporter of the mitochondrion 3; AtATM3; Iron-sulfur clusters transporter ATM3; Protein STARIK 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 83% coverage: 12:228/261 of query aligns to 487:700/728 of Q9LVM1

query
sites
Q9LVM1

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R612 (= R139) mutation to K: Resistant to sirtinol and reduced chlorophyll content.

4f4cA The crystal structure of the multi-drug transporter (see paper)
34% identity, 83% coverage: 15:230/261 of query aligns to 1031:1249/1250 of 4f4cA

query
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4f4cA

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E

L

G

Y

F

S

E

M

T

A

R

D

V

R

G

A

D

F

R

G

G

T

T

-

Q

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

I

M

A

V

I

A

A

R

R

A

A

L

L

A

V

Q

R

E

N

P

P

S

K

L

I

L

L

V

L

L

L

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

E

H

S

Q

E

-

K

-

V

L

Q

D

E

V

A

L

L

D

D

L

R

I

A

R

R

D

E

L

G

D

R

V

T

T

C

I

I

V

V

T

I

S

A

L

-

H

H

D

R

L

L

N

N

I

T

A

V

A

M

G

N

A

A

C

-

D

D

E

C

I

I

L

A

L

V

M

V

S

S

E

N

G

G

R

T

Q

I

L

I

A

E

F

K

G

G

T

T

P

H

H

T

D

Q

V

L

L

M

S

S

E

E

Sites not aligning to the query:

binding undecyl 4-O-alpha-D-glucopyranosyl-1-thio-beta-D-glucopyranoside: 19, 857, 857, 858, 860, 934, 973, 976, 976, 979

7n5aA Structure of atatm3 in the closed conformation (see paper)
33% identity, 83% coverage: 12:228/261 of query aligns to 360:573/589 of 7n5aA

query
sites
7n5aA

S

S

W
x
Y

G

L

P

P

G

E

R

R

A

K

A

-

P

-

L

-

I

I

L

L

H

D

P

G

I

I

S

S

F

F

H

V

L

V

D

P

A

A

G

G

R

K

V

S

L

V

G

A

V

I

V

V

G

G

P

T

N

S

G

G

A

S

G
|
G

K
|
K

S
|
S

S
x
T

L

I

L

L

R

R

V

M

I

L

Y

F

R

R

F

F

N

F

Q

D

P

T

R

D

S

S

G

G

K

N

V

I

E

R

V

I

G

D

D

G

S

Q

D

D

I

I

W

K

A

E

L

V

P

R

P

L

K

D

A

S

A

L

A

R

Q

S

R

S

V

I

A

G

A

V

V

V

L

P

Q

Q

E

D

Q

T

P

V

T

L

D

-

F

F

A

N

L

D

T

T

V

I

A

F

E

H

I

N

V

I

A

H

L

Y

G

G

R

R

A

L

P

S

Y

A

R

T

S

E

G

E

F

E

A

V

T

Y

P

E

G

A

A

A

R

R

D

R

A

A

I

I

I

H

E

E

G

T

M

I

L

S

D

N

R

F

L

P

D

D

L

K

Y

Y

S

S

-

T

-

I

M

V

A

G

D

E

R

R

A

G

F

L

G

-

T
x
K

L

L

S
|
S

G

G

E
|
E

R

K

Q

Q

R

R

V

V

M

A

V

L

A

A

R

R

A

T

L

F

A

L

Q

K

E

S

P

P

S

A

L

I

L

L

V

L

L

C

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

S

R

T

H

T

Q

E

L

A

D

E

V

I

L

L

D

N

L

A

I

L

R

K

D

A

L

L

-

A

-

S

D

N

V

R

T

T

I

S

V

I

T

F

S

I

L

A

H

H

D

R

L

L

N

T

I

T

A

A

A

M

G

-

A

Q

C

C

D

D

E

E

I

I

L

V

M

L

S

E

E

N

G

G

R

K

Q

V

L

V

A

E

F

Q

G

G

T

-

P

P

H

H

D

D

V

E

L

L

binding adenosine-5'-diphosphate: Y361 (≠ W13), G390 (= G45), K391 (= K46), S392 (= S47), T393 (≠ S48), K488 (≠ T143), S490 (= S145), E493 (= E148)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
32% identity, 85% coverage: 22:242/261 of query aligns to 17:238/241 of 4u00A

query
sites
4u00A

I
x
V

L

L

H

K

P

G

I

I

S

H

F

L

H

E

L

V

D

A

A

P

G

G

R

E

V

K

L

L

G

V

V

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

R

R

V

T

I

I

Y

N

R

R

F

L

N

E

Q

D

P

F

R

Q

S

E

G

G

K

E

V

V

E

V

V

V

G

D

D

G

-

L

-

S

-

V

S

K

D

D

I

D

W

R

A

A

L

L

P

-

K

R

A

E

A

I

A

R

Q

R

R

E

V

V

A

G

A

M

V

V

L

F

Q

Q

E

Q

Q

F

P

N

T

L

D

F

F

P

A

H

L

M

T

T

V

V

A

L

E

E

I

N

V

V

A

T

L

L

G

-

R

-

A

A

P

P

Y

M

R

R

S

V

G

R

F

R

A

W

T

P

P

R

G

E

A

K

R

A

D

E

A

K

I

A

I

L

E

E

G

-

M

L

L

L

D

E

R

R

L

V

D

G

L

I

Y

L

S

D

M

Q

A

A

D

R

R

K

A

Y

F

P

G

A

T

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

A

L

L

A

A

Q

M

E

E

P

P

S

K

L

I

L

M

V

L

L

F

D

D

E

E

P

P

T

T

N

S

H

A

L

L

D

D

I

P

R

E

H

M

Q

V

L

G

D

E

V

V

L

L

D

D

L

V

I

M

R

R

D

D

L

L

-

A

-

Q

-

G

D

G

V

M

T

T

I

M

V

V

T

V

S

V

L

T

H

H

D

E

L

M

N

G

I

F

A

A

A

R

G

E

A

V

C

A

D

D

E

R

I

V

L

V

L

F

M

M

S

D

E

G

G

G

R

Q

Q

I

L

V

A

E

F

E

G

G

T

R

P

P

H

E

D

E

V

I

L

F

S

T

E

R

D

P

T

K

V

E

S

E

R

R

V

T

F

R

N

S

V

F

A

L

A

Q

R

R

binding adenosine-5'-diphosphate: V17 (≠ I22), S37 (≠ N42), G38 (= G43), S39 (≠ A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

7n59A Structure of atatm3 in the inward-facing conformation with gssg bound (see paper)
33% identity, 83% coverage: 12:228/261 of query aligns to 361:574/590 of 7n59A

query
sites
7n59A

S

S

W

Y

G

L

P

P

G

E

R

R

A

K

A

-

P

-

L

-

I

I

L

L

H

D

P

G

I

I

S

S

F

F

H

V

L

V

D

P

A

A

G

G

R

K

V

S

L

V

G

A

V

I

V

V

G

G

P

T

N

S

G

G

A

S

G

G

K

K

S

S

S

T

L

I

L

L

R

R

V

M

I

L

Y

F

R

R

F

F

N

F

Q

D

P

T

R

D

S

S

G

G

K

N

V

I

E

R

V

I

G

D

D

G

S

Q

D

D

I

I

W

K

A

E

L

V

P

R

P

L

K

D

A

S

A

L

A

R

Q

S

R

S

V

I

A

G

A

V

V

V

L

P

Q

Q

E

D

Q

T

P

V

T

L

D

-

F

F

A

N

L

D

T

T

V

I

A

F

E

H

I

N

V

I

A

H

L

Y

G

G

R

R

A

L

P

S

Y

A

R

T

S

E

G

E

F

E

A

V

T

Y

P

E

G

A

A

A

R

R

D

R

A

A

I

I

I

H

E

E

G

T

M

I

L

S

D

N

R

F

L

P

D

D

L

K

Y

Y

S

S

-

T

-

I

M

V

A

G

D

E

R

R

A

G

F

L

G

-

T

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

M

A

V

L

A

A

R

R

A

T

L

F

A

L

Q

K

E

S

P

P

S

A

L

I

L

L

V

L

L

C

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

S

R

T

H

T

Q

E

L

A

D

E

V

I

L

L

D

N

L

A

I

L

R

K

D

A

L

L

-

A

-

S

D

N

V

R

T

T

I

S

V

I

T

F

S

I

L

A

H

H

D

R

L

L

N

T

I

T

A

A

A

M

G

-

A

Q

C

C

D

D

E

E

I

I

L

V

M

L

S

E

E

N

G

G

R

K

Q

V

L

V

A

E

F

Q

G

G

T

-

P

P

H

H

D

D

V

E

L

L

Sites not aligning to the query:

binding oxidized glutathione disulfide: 202, 257, 264, 308

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 83% coverage: 23:238/261 of query aligns to 21:230/343 of P30750

query
sites
P30750

L

L

H

N

P

N

I

V

S

S

F

L

H

H

L

V

D

P

A

A

G

G

R

Q

V

I

L

Y

G

G

V

V

V

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

R

R

V

C

I

V

Y

N

R

L

F

L

N

E

Q

R

P

P

R

T

S

E

G

G

K

S

V

V

E

L

V

V

G

D

D

G

S

Q

D

E

I

L

W

T

A

T

L

L

P

S

P

E

K

S

-

E

-

L

-

T

A

K

A

A

A

R

Q

R

R

Q

V

I

A

G

A

M

V

I

L

F

Q

Q

E

H

Q

F

P

N

T

L

D

L

F

S

A

S

L

R

T

T

V

V

A

F

E

G

I

N

V

V

A

A

L

L

G

-

R

-

A

-

P

P

Y

L

R

E

S

L

G

D

F

-

A

N

T

T

P

P

G

K

A

D

R

E

D

V

A

K

A

R

I

R

I

V

E

T

G

E

M

L

L

L

D

S

R

L

L

V

D

G

L

L

Y

G

S

D

M

K

A

H

D

D

R

S

A

Y

F

P

G

S

T

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

S

K

L

V

L

L

V

L

L

C

D

D

E
|
E

P

A

T

T

N

S

H

A

L

L

D

D

I

P

R

A

H

T

Q

T

L

R

D

S

V

I

L

L

D

E

L

L

I

L

R

K

D

D

L

I

D

N

-

R

-

L

-

G

V

L

T

T

I

I

V

L

T

L

S

I

L

T

H

H

D

E

L

M

N

D

I

V

A

V

A

K

G

R

A

I

C

C

D

D

E

C

I

V

L

A

L

V

M

I

S

S

E

N

G

G

R

-

Q

-

L

-

A

-

F

-

G

-

T

-

P

-

H

-

D

E

V

L

L

I

S

E

E

Q

D

D

T

T

V

V

S

S

R

E

V

V

F

F

N

S

40:46 (vs. 42:48, 71% identical) binding
E166 (= E169) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

O06967 Multidrug resistance ABC transporter ATP-binding/permease protein BmrA; EC 7.6.2.- from Bacillus subtilis (strain 168) (see 2 papers)
30% identity, 87% coverage: 11:237/261 of query aligns to 346:573/589 of O06967

query
sites
O06967

V

V

S

S

W

F

G

G

P

Y

G

-

R

K

A

P

A

D

P

Q

L

L

I

I

L

L

H

K

P

E

I

V

S

S

F

A

H

V

L

I

D

E

A

A

G

G

R

K

V

V

L

T

G

A

V

I

V

V

G

G

P

P

N

S

G

G

A

G

G

G

K
|
K

S

T

L

L

L

F

R

K

V

L

I

L

Y

E

R

R

F

F

N

Y

Q

S

P

P

R

T

S

A

G

G

K

T

V

I

E

R

V

L

G

G

D

D

S

E

D

P

I

V

W

D

A

T

L

Y

P

S

P

L

K

E

A

S

A

W

A

R

Q

E

R

H

V

I

A

G

A

Y

V

V

L

S

Q

Q

E

E

Q

S

P

P

T

L

D

-

F

M

A

S

L

G

T

T

V

I

A

R

E

E

I

N

V

I

A

C

L

Y

G

G

R

L

A

E

P

R

Y

D

R

V

S

T

G

D

F

A

A

E

T

I

P

E

G

K

A

A

R

A

D

E

A

M

A

A

-

Y

-

A

-

L

-

N

I

F

I

I

E

K

G

E

M

L

L

P

D

N

R

Q

L

F

D

D

L

T

Y

-

S

E

M

V

A

G

D

E

R

R

A

G

F

I

G

-

T

M

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

I

M

A

V

I

A

A

R

R

A

A

L

L

A

L

Q

R

E

N

P

P

S

S

L

I

L

L

V

M

L

L

D

D

E
|
E

P

A

T

T

N

S

H

S

L

L

D

D

I

S

R

Q

H

S

Q

E

L

K

D

S

V

V

-

Q

-

A

L

L

D

E

L

V

I

L

R

M

D

E

L

G

D

R

V

T

T

T

I

I

V

V

T

I

S

A

L

-

H

H

D

R

L

L

N

S

I

T

A

V

G

D

A

A

C

-

D

D

E

Q

I

L

L

L

L

F

M

V

S

E

E

K

G

G

R

E

Q

I

L

T

A

G

F

R

G

G

T

T

P

H

H

H

D

E

V

L

L

M

S

A

E

S

D

H

T

G

V

L

S

Y

R

R

V

D

F

F

K380 (= K46) mutation to A: Complete loss of ATPase activity.; mutation to R: Retains 2% ATPase activity; unable to transport Hoechst 33342. Traps ADP in a beryllium fluoride-dependent manner, confirming ATPase activity. Probably unable to undergo NBD dimerization.
E504 (= E169) mutation E->A,C,D,Q,S: Complete loss of ATPase activity; mutant proteins trap ATP in a vanadate-independent manner whereas the wild-type protein traps ADP.

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
30% identity, 83% coverage: 23:238/261 of query aligns to 22:231/344 of 6cvlD

query
sites
6cvlD

L

L

H

N

P

N

I

V

S

S

F

L

H

H

L

V

D

P

A

A

G

G

R

Q

V

I

L

Y

G

G

V

V

V

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

R

R

V

C

I

V

Y

N

R

L

F

L

N

E

Q

R

P

P

R

T

S

E

G

G

K

S

V

V

E

L

V

V

G

D

D

G

S

Q

D

E

I

L

W

T

A

T

L

L

P

S

P

E

K

S

-

E

-

L

-

T

A

K

A

A

A

R

Q

R

R

Q

V

I

A

G

A

M

V

I

L

F

Q

Q

E

H

Q

F

P

N

T

L

D

L

F

S

A

S

L

R

T

T

V

V

A

F

E

G

I

N

V

V

A

A

L

L

G

-

R

-

A

-

P

P

Y

L

R

E

S

L

G

D

F

-

A

N

T

T

P

P

G

K

A

D

R

E

D

V

A

K

A

R

I

R

I

V

E

T

G

E

M

L

L

L

D

S

R

L

L

V

D

G

L

L

Y

G

S

D

M

K

A

H

D

D

R

S

A

Y

F

P

G

S

T
x
N

L

L

S
|
S

G

G

E
x
Q

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

S

K

L

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

N

S

H

A

L

L

D

D

I

P

R

A

H

T

Q

T

L

R

D

S

V

I

L

L

D

E

L

L

I

L

R

K

D

D

L

I

D

N

-

R

-

L

-

G

V

L

T

T

I

I

V

L

T

L

S

I

L

T

H
|
H

D

E

L

M

N

D

I

V

A

V

A

K

G

R

A

I

C

C

D

D

E

C

I

V

L

A

L

V

M

I

S

S

E

N

G

G

R

-

Q

-

L

-

A

-

F

-

G

-

T

-

P

-

H

-

D

E

V

L

L

I

S

E

E

Q

D

D

T

T

V

V

S

S

R

E

V

V

F

F

N

S

binding phosphothiophosphoric acid-adenylate ester: S41 (≠ N42), G42 (= G43), A43 (= A44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (≠ S48), N141 (≠ T143), S143 (= S145), Q146 (≠ E148), H200 (= H198)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
30% identity, 83% coverage: 23:238/261 of query aligns to 22:231/344 of 3tuzC

query
sites
3tuzC

L

L

H

N

P

N

I

V

S

S

F

L

H

H

L

V

D

P

A

A

G

G

R

Q

V

I

L

Y

G

G

V

V

V

I

G

G

P

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

R

R

V

C

I

V

Y

N

R

L

F

L

N

E

Q

R

P

P

R

T

S

E

G

G

K

S

V

V

E

L

V

V

G

D

D

G

S

Q

D

E

I

L

W

T

A

T

L

L

P

S

P

E

K

S

-

E

-

L

-

T

A

K

A

A

A

R

Q

R

R

Q

V

I

A

G

A

M

V

I

L

F

Q

Q

E

H

Q

F

P

N

T

L

D

L

F

S

A

S

L

R

T

T

V

V

A

F

E

G

I

N

V

V

A

A

L

L

G

-

R

-

A

-

P

P

Y

L

R

E

S

L

G

D

F

-

A

N

T

T

P

P

G

K

A

D

R

E

D

V

A

K

A

R

I

R

I

V

E

T

G

E

M

L

L

L

D

S

R

L

L

V

D

G

L

L

Y

G

S

D

M

K

A

H

D

D

R

S

A

Y

F

P

G

S

T

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

S

K

L

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

N

S

H

A

L

L

D

D

I

P

R

A

H

T

Q

T

L

R

D

S

V

I

L

L

D

E

L

L

I

L

R

K

D

D

L

I

D

N

-

R

-

L

-

G

V

L

T

T

I

I

V

L

T

L

S

I

L

T

H

H

D

E

L

M

N

D

I

V

A

V

A

K

G

R

A

I

C

C

D

D

E

C

I

V

L

A

L

V

M

I

S

S

E

N

G

G

R

-

Q

-

L

-

A

-

F

-

G

-

T

-

P

-

H

-

D

E

V

L

L

I

S

E

E

Q

D

D

T

T

V

V

S

S

R

E

V

V

F

F

N

S

binding adenosine-5'-diphosphate: G42 (= G43), G44 (= G45), K45 (= K46), S46 (= S47), T47 (≠ S48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
30% identity, 83% coverage: 23:238/261 of query aligns to 22:231/344 of 3tuiC

query
sites
3tuiC

L

L

H

N

P

N

I

V

S

S

F

L

H

H

L

V

D

P

A

A

G

G

R

Q

V

I

L

Y

G

G

V

V

V

I

G

G

P

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

R

R

V

C

I

V

Y

N

R

L

F

L

N

E

Q

R

P

P

R

T

S

E

G

G

K

S

V

V

E

L

V

V

G

D

D

G

S

Q

D

E

I

L

W

T

A

T

L

L

P

S

P

E

K

S

-

E

-

L

-

T

A

K

A

A

A

R

Q

R

R

Q

V

I

A

G

A

M

V

I

L

F

Q

Q

E

H

Q

F

P

N

T

L

D

L

F

S

A

S

L

R

T

T

V

V

A

F

E

G

I

N

V

V

A

A

L

L

G

-

R

-

A

-

P

P

Y

L

R

E

S

L

G

D

F

-

A

N

T

T

P

P

G

K

A

D

R

E

D

V

A

K

A

R

I

R

I

V

E

T

G

E

M

L

L

L

D

S

R

L

L

V

D

G

L

L

Y

G

S

D

M

K

A

H

D

D

R

S

A

Y

F

P

G

S

T

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

A

L

L

A

A

Q

S

E

N

P

P

S

K

L

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

N

S

H

A

L

L

D

D

I

P

R

A

H

T

Q

T

L

R

D

S

V

I

L

L

D

E

L

L

I

L

R

K

D

D

L

I

D

N

-

R

-

L

-

G

V

L

T

T

I

I

V

L

T

L

S

I

L

T

H

H

D

E

L

M

N

D

I

V

A

V

A

K

G

R

A

I

C

C

D

D

E

C

I

V

L

A

L

V

M

I

S

S

E

N

G

G

R

-

Q

-

L

-

A

-

F

-

G

-

T

-

P

-

H

-

D

E

V

L

L

I

S

E

E

Q

D

D

T

T

V

V

S

S

R

E

V

V

F

F

N

S

binding adenosine-5'-diphosphate: G42 (= G43), A43 (= A44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (≠ S48)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

8i6rB Cryo-em structure of pseudomonas aeruginosa ftse(e163q)x/envc complex with atp in peptidisc (see paper)
31% identity, 76% coverage: 23:220/261 of query aligns to 18:217/222 of 8i6rB

query
sites
8i6rB

L

L

H

H

P

E

I

V

S

S

F

F

H

R

L

V

D

H

A

R

G

G

R

E

V

I

L

L

G

F

V

V

V

T

G

G

P

H

N
x
S

G

G

A
|
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

V

L

I

I

Y

L

R

A

F

M

N

E

Q

R

P

P

R

T

S

S

G

G

K

K

V

L

E

L

V

L

G

G

D

G

S

Q

D

D

I

L

W

G

A

R

L

I

P

T

K

A

A

Q

A

I

-

P

-

F

-

L

A

R

Q

R

R

Q

V

I

A

G

A

V

V

V

L

F

Q

Q

E

N

Q

H

P

Q

T

L

D

L

F

T

A

D

L

R

T

T

V

V

A

A

E

D

I

N

V

I

A

A

L

L

G

-

R

-

A

-

P

P

Y

L

R

Q

S

I

G

-

F

L

A

G

T

M

P

P

G

K

A

P

R

E

D

I

A

A

A

K

I

R

I

V

E

A

G

S

M

A

L

L

D

E

R

R

L

V

D

N

L

L

Y

K

S

E

M

K

A

G

D

E

R

A

A

L

F

P

G

S

T
x
D

L

L

S
|
S

G
x
T

G
|
G

E
x
Q

R

Q

Q

Q

R

R

V

V

M

G

V

I

A

A

R

R

A

A

L

I

A

V

Q

H

E

Q

P

P

S

A

L

L

V

L

L

A

D

D

E

Q

P

P

T

T

N

G

H

N

L

L

D

D

I

P

R

R

H

L

Q

A

L

S

D

E

V

I

L

M

D

G

L

V

I

F

R

E

D

D

L

I

D

N

-

R

-

L

-

G

V

T

T

T

I

V

V

L

T

I

S

A

L

S

H

H

D

D

L

L

N

A

I

L

A

I

A

A

G

R

A

M

C

R

D

H

E

R

I

M

L

L

L

T

M

L

S

Q

E

R

G

G

R

R

Q

I

L

I

A

A

binding adenosine-5'-triphosphate: S37 (≠ N42), A39 (= A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48), D137 (≠ T143), S139 (= S145), T140 (≠ G146), G141 (= G147), Q142 (≠ E148)
binding magnesium ion: K41 (= K46), S42 (= S47)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11, 15

4mrvA Structure of a bacterial atm1-family abc transporter (see paper)
31% identity, 87% coverage: 5:231/261 of query aligns to 353:579/600 of 4mrvA

query
sites
4mrvA

S

S

L

V

T

T

V

F

T

D

D

N

V

V

S

V

W

F

G

G

P

Y

G

D

R

R

A

D

A

R

P

E

L

-

I

I

L

L

H

H

P

G

I

L

S

S

F

F

H

E

L

V

D

A

A

A

G

G

R

S

V

R

L

V

G

A

V

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K

K

S
|
S

S

T

L

I

L

A

R

R

V

L

I

L

Y

F

R

R

F

F

N

Y

Q

D

P

P

R

W

S

E

G

G

K

R

V

I

E

L

V

I

G

D

D

G

S

Q

D

D

I

I

W

-

A

A

L

H

P

V

P

T

K

Q

A

T

A

S

A

L

Q

R

R

A

V

A

A

L

A

G

V

I

L

V

Q

P

E

Q

Q

D

P

S

T

V

D

L

F

F

A

N

L

D

T

T

V

I

A

G

E

Y

I

N

V

I

A

A

L

Y

G

G

R

R

A

-

P

-

Y

-

R

-

S

-

G

-

F

-

A

-

T

-

P

D

G

G

A

A

R

S

D

R

A

A

A

E

I

V

-

D

-

A

I

A

E

A

G

K

M

G

L

A

D

A

R

I

L

A

D

D

L

F

Y

I

S

A

M

R

A

L

D

P

R

Q

A

G

F

Y

G

D

T

T

-

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

T

L

L

A

V

Q

K

E

N

P

P

S

P

L

I

L

L

V

L

L

F

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

R

H

T

Q

E

L

Q

D

D

V

I

L

L

D

S

L

T

I

M

R

R

D

A

L

V

-

A

-

S

D

H

V

R

T

T

I

T

V

I

T

S

S

I

L

A

H

H

D

R

L

L

N

S

I

T

A

I

A

A

G

D

A

S

C

-

D

D

E

T

I

I

L

L

L

V

M

L

S

D

E

Q

G

G

R

R

Q

L

L

A

A

E

F

Q

G

G

T

S

P

H

H

L

D

D

V

L

L

L

S

R

E

R

D

D

binding phosphate ion: S389 (≠ N42), G392 (= G45), S394 (= S47)

Sites not aligning to the query:

binding mercury bis(L-gamma-glutamyl-3-sulfido-L-alanylglycine): 149, 261, 262, 265, 309, 310, 311, 312, 313
binding phosphate ion: 313, 316

4mrsA Structure of a bacterial atm1-family abc transporter (see paper)
31% identity, 87% coverage: 5:231/261 of query aligns to 353:579/600 of 4mrsA

query
sites
4mrsA

S

S

L

V

T

T

V

F

T

D

D

N

V

V

S

V

W

F

G

G

P

Y

G

D

R

R

A

D

A

R

P

E

L

-

I

I

L

L

H

H

P

G

I

L

S

S

F

F

H

E

L

V

D

A

A

A

G

G

R

S

V

R

L

V

G

A

V

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K

K

S
|
S

S

T

L

I

L

A

R

R

V

L

I

L

Y

F

R

R

F

F

N

Y

Q

D

P

P

R

W

S

E

G

G

K

R

V

I

E

L

V

I

G

D

D

G

S

Q

D

D

I

I

W

-

A

A

L

H

P

V

P

T

K

Q

A

T

A

S

A

L

Q

R

R

A

V

A

A

L

A

G

V

I

L

V

Q

P

E

Q

Q

D

P

S

T

V

D

L

F

F

A

N

L

D

T

T

V

I

A

G

E

Y

I

N

V

I

A

A

L

Y

G

G

R

R

A

-

P

-

Y

-

R

-

S

-

G

-

F

-

A

-

T

-

P

D

G

G

A

A

R

S

D

R

A

A

A

E

I

V

-

D

-

A

I

A

E

A

G

K

M

G

L

A

D

A

R

I

L

A

D

D

L

F

Y

I

S

A

M

R

A

L

D

P

R

Q

A

G

F

Y

G

D

T

T

-

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

T

L

L

A

V

Q

K

E

N

P

P

S

P

L

I

L

L

V

L

L

F

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

R

H

T

Q

E

L

Q

D

D

V

I

L

L

D

S

L

T

I

M

R

R

D

A

L

V

-

A

-

S

D

H

V

R

T

T

I

T

V

I

T

S

S

I

L

A

H

H

D

R

L

L

N

S

I

T

A

I

A

A

G

D

A

S

C

-

D

D

E

T

I

I

L

L

L

V

M

L

S

D

E

Q

G

G

R

R

Q

L

L

A

A

E

F

Q

G

G

T

S

P

H

H

L

D

D

V

L

L

L

S

R

E

R

D

D

binding phosphate ion: S389 (≠ N42), G392 (= G45), S394 (= S47)

Sites not aligning to the query:

binding oxidized glutathione disulfide: 142, 149, 199, 203, 261, 262, 265, 309, 310, 312, 313

4mrpA Structure of a bacterial atm1-family abc transporter (see paper)
31% identity, 87% coverage: 5:231/261 of query aligns to 353:579/600 of 4mrpA

query
sites
4mrpA

S

S

L

V

T

T

V

F

T

D

D

N

V

V

S

V

W

F

G

G

P

Y

G

D

R

R

A

D

A

R

P

E

L

-

I

I

L

L

H

H

P

G

I

L

S

S

F

F

H

E

L

V

D

A

A

A

G

G

R

S

V

R

L

V

G

A

V

I

V

V

G

G

P

P

N
x
S

G
|
G

A

A

G
|
G

K

K

S
|
S

S

T

L

I

L

A

R

R

V

L

I

L

Y

F

R

R

F

F

N

Y

Q

D

P

P

R

W

S

E

G

G

K

R

V

I

E

L

V

I

G

D

D

G

S

Q

D

D

I

I

W

-

A

A

L

H

P

V

P

T

K

Q

A

T

A

S

A

L

Q

R

R

A

V

A

A

L

A

G

V

I

L

V

Q

P

E

Q

Q

D

P

S

T

V

D

L

F

F

A

N

L

D

T

T

V

I

A

G

E

Y

I

N

V

I

A

A

L

Y

G

G

R

R

A

-

P

-

Y

-

R

-

S

-

G

-

F

-

A

-

T

-

P

D

G

G

A

A

R

S

D

R

A

A

A

E

I

V

-

D

-

A

I

A

E

A

G

K

M

G

L

A

D

A

R

I

L

A

D

D

L

F

Y

I

S

A

M

R

A

L

D

P

R

Q

A

G

F

Y

G

D

T

T

-

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

T

L

L

A

V

Q

K

E

N

P

P

S

P

L

I

L

L

V

L

L

F

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

R

H

T

Q

E

L

Q

D

D

V

I

L

L

D

S

L

T

I

M

R

R

D

A

L

V

-

A

-

S

D

H

V

R

T

T

I

T

V

I

T

S

S

I

L

A

H

H

D

R

L

L

N

S

I

T

A

I

A

A

G

D

A

S

C

-

D

D

E

T

I

I

L

L

L

V

M

L

S

D

E

Q

G

G

R

R

Q

L

L

A

A

E

F

Q

G

G

T

S

P

H

H

L

D

D

V

L

L

L

S

R

E

R

D

D

binding phosphate ion: S389 (≠ N42), G390 (= G43), G392 (= G45), S394 (= S47)

Sites not aligning to the query:

binding glutathione: 149, 261, 262, 265, 309, 310, 310, 311, 312, 313

4mrnA Structure of a bacterial atm1-family abc transporter (see paper)
31% identity, 87% coverage: 5:231/261 of query aligns to 353:579/600 of 4mrnA

query
sites
4mrnA

S

S

L

V

T

T

V

F

T

D

D

N

V

V

S

V

W

F

G

G

P

Y

G

D

R

R

A

D

A

R

P

E

L

-

I

I

L

L

H

H

P

G

I

L

S

S

F

F

H

E

L

V

D

A

A

A

G

G

R

S

V

R

L

V

G

A

V

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K
|
K

S
|
S

S

T

L

I

L

A

R

R

V

L

I

L

Y

F

R

R

F

F

N

Y

Q

D

P

P

R

W

S

E

G

G

K

R

V

I

E

L

V

I

G

D

D

G

S

Q

D

D

I

I

W

-

A

A

L

H

P

V

P

T

K

Q

A

T

A

S

A

L

Q

R

R

A

V

A

A

L

A

G

V

I

L

V

Q

P

E

Q

Q

D

P

S

T

V

D

L

F

F

A

N

L

D

T

T

V

I

A

G

E

Y

I

N

V

I

A

A

L

Y

G

G

R

R

A

-

P

-

Y

-

R

-

S

-

G

-

F

-

A

-

T

-

P

D

G

G

A

A

R

S

D

R

A

A

A

E

I

V

-

D

-

A

I

A

E

A

G

K

M

G

L

A

D

A

R

I

L

A

D

D

L

F

Y

I

S

A

M

R

A

L

D

P

R

Q

A

G

F

Y

G

D

T

T

-

E

-

V

-

G

-

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

T

L

L

A

V

Q

K

E

N

P

P

S

P

L

I

L

L

V

L

L

F

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

R

H

T

Q

E

L

Q

D

D

V

I

L

L

D

S

L

T

I

M

R

R

D

A

L

V

-

A

-

S

D

H

V

R

T

T

I

T

V

I

T

S

S

I

L

A

H

H

D

R

L

L

N

S

I

T

A

I

A

A

G

D

A

S

C

-

D

D

E

T

I

I

L

L

L

V

M

L

S

D

E

Q

G

G

R

R

Q

L

L

A

A

E

F

Q

G

G

T

S

P

H

H

L

D

D

V

L

L

L

S

R

E

R

D

D

binding phosphate ion: S389 (≠ N42), G392 (= G45), K393 (= K46), S394 (= S47)

6parA Structure of a bacterial atm1-family abc exporter with mgamppnp bound (see paper)
31% identity, 87% coverage: 5:231/261 of query aligns to 339:565/578 of 6parA

query
sites
6parA

S

S

L

V

T

T

V

F

T

D

D

N

V

V

S

V

W

F

G

G

P
x
Y

G

D

R

R

A

D

A
x
R

P

E

L

-

I
|
I

L

L

H

H

P

G

I

L

S

S

F

F

H

E

L

V

D

A

A

A

G

G

R

S

V

R

L

V

G

A

V

I

V

V

G

G

P

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

S
x
T

L

I

L

A

R

R

V

L

I

L

Y

F

R

R

F

F

N

Y

Q

D

P

P

R

W

S

E

G

G

K

R

V

I

E

L

V

I

G

D

D

G

S

Q

D

D

I

I

W

-

A

A

L

H

P

V

P

T

K

Q

A

T

A

S

A

L

Q

R

R

A

V

A

A

L

A

G

V

I

L

V

Q

P

E
x
Q

Q

D

P

S

T

V

D

L

F

F

A

N

L

D

T

T

V

I

A

G

E

Y

I

N

V

I

A

A

L

Y

G

G

R

R

A

-

P

-

Y

-

R

-

S

-

G

-

F

-

A

-

T

-

P

D

G

G

A

A

R

S

D

R

A

A

A

E

I

V

-

D

-

A

I

A

E

A

G

K

M

G

L

A

D

A

R

I

L

A

D

D

L

F

Y

I

S

A

M

R

A

L

D

P

R

Q

A

G

F

Y

G

D

T

T

-

E

-

V

-

G

-

E

-

R

-

G

-

L

-
x
K

L

L

S
|
S

G
|
G

E
|
E

R

K

Q

Q

R

R

V

V

M

A

V

I

A

A

R

R

A

T

L

L

A

V

Q

K

E

N

P

P

S

P

L

I

L

L

V

L

L

F

D

D

E

E

P

A

T

T

N

S

H

A

L

L

D

D

I

T

R

R

H

T

Q

E

L

Q

D

D

V

I

L

L

D

S

L

T

I

M

R

R

D

A

L

V

-

A

-

S

D

H

V

R

T

T

I

T

V

I

T

S

S

I

L

A

H

H

D

R

L

L

N

S

I

T

A

I

A

A

G

D

A

S

C

-

D

D

E

T

I

I

L

L

L

V

M

L

S

D

E

Q

G

G

R

R

Q

L

L

A

A

E

F

Q

G

G

T

S

P

H

H

L

D

D

V

L

L

L

S

R

E

R

D

D

binding phosphoaminophosphonic acid-adenylate ester: Y349 (≠ P15), R353 (≠ A19), I355 (= I22), S375 (≠ N42), G376 (= G43), G378 (= G45), K379 (= K46), S380 (= S47), T381 (≠ S48), K476 (vs. gap), S478 (= S145), G479 (= G146), G480 (= G147), E481 (= E148)
binding magnesium ion: S380 (= S47), Q421 (≠ E89)

Query Sequence

>GFF2646 FitnessBrowser__Phaeo:GFF2646
MTAASLTVTDVSWGPGRAAPLILHPISFHLDAGRVLGVVGPNGAGKSSLLRVIYRFNQPR
SGKVEVGDSDIWALPPKAAAQRVAAVLQEQPTDFALTVAEIVALGRAPYRSGFATPGARD
AAIIEGMLDRLDLYSMADRAFGTLSGGERQRVMVARALAQEPSLLVLDEPTNHLDIRHQL
DVLDLIRDLDVTIVTSLHDLNIAAGACDEILLMSEGRQLAFGTPHDVLSEDTVSRVFNVA
ARRETLTPSGQDHLTFHLPVS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory