SitesBLAST

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
37% identity, 84% coverage: 18:256/284 of query aligns to 16:257/260 of P02911

query
sites
P02911

A

A

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D33 (≠ A35) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y38) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (≠ E54) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (= C62) modified: Disulfide link with 67
C67 (= C69) modified: Disulfide link with 60
F74 (≠ W76) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (= S93) binding ; binding ; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (= S94) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
S94 (= S96) binding ; binding ; binding ; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R101) binding ; binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (vs. gap) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (= T144) binding ; binding ; binding
D183 (= D184) binding ; binding ; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
38% identity, 77% coverage: 38:256/284 of query aligns to 11:232/235 of 5owfA

query
sites
5owfA

Y
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Y

P

A

P

P

F

F

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K

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binding glutamine: Y11 (= Y38), F49 (≠ W76), S66 (= S93), S67 (= S94), S69 (= S96), R74 (= R101), K114 (vs. gap), S117 (= S143), T118 (= T144), D158 (= D184)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
38% identity, 77% coverage: 38:256/284 of query aligns to 14:235/238 of 1lstA

query
sites
1lstA

Y
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Y

P

A

P

P

F

F

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V

S

K

K

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K

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x
F

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A

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binding lysine: Y14 (= Y38), F52 (≠ W76), S70 (= S94), S72 (= S96), R77 (= R101), L117 (vs. gap), S120 (= S143), T121 (= T144), Q122 (≠ V145), D161 (= D184)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 77% coverage: 38:256/284 of query aligns to 14:235/238 of 1lahE

query
sites
1lahE

Y
|
Y

P

A

P

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F

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K

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x
Q

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A

A

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Y

Y

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N

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D
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D

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A

A

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A

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M

E

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G

M

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A

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A

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F

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A

A

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L

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D

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E

L

L

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K

A

A

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A

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F

D

D

K

K

A

A

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E

L

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D

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Y

Y

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K

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K

Y

Y

F

F

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D

L

F

S

N

V

V

binding L-ornithine: Y14 (= Y38), F52 (≠ W76), S70 (= S94), S72 (= S96), R77 (= R101), S120 (= S143), Q122 (≠ V145), D161 (= D184)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 77% coverage: 38:256/284 of query aligns to 14:235/238 of 1lagE

query
sites
1lagE

Y
|
Y

P

A

P

P

F

F

T

S

V

S

K

K

G

D

G

A

N

K

G

G

Q

E

W

F

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F

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D

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L

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K

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A

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x
F

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D

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A

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S

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L

L

K

A

A

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S

M
x
L

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R
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R

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Q

K

Q

Q

E

I

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A

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F

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Y

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L

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K

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G

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V

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x
L

Q

Q

I

G

S
|
S

T

T

V
x
Q

S

E

A

A

N

Y

Y

A

L

N

K

D

K

N

Y

W

Y

R

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T

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K

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D

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Y

A

Y

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D

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Y

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D

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L

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A

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D

Y

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M

A

M

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A

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D
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D

D

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T

V

A

A

I

A

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M

E

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G

M

F

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A

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E

L

Y

A

A

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F

I

A

A

G

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P

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S

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V

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K

Y

D

D

K

P

K

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Y

I

F

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R

D

G

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T

G

G

A

V

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G

L

L

R

R

K

K

E

D

D

D

T

T

A

E

L

L

K

K

A

A

R

A

L

F

D

D

K

K

A

A

I

L

G

T

E

E

L

L

L

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V

Q

S

D

K

G

D

T

Y

Y

D

D

D

K

L

M

S

A

Q

K

H

K

Y

Y

F

F

G

D

L

F

S

N

V

V

binding histidine: Y14 (= Y38), F52 (≠ W76), S69 (= S93), S70 (= S94), L71 (≠ M95), S72 (= S96), R77 (= R101), L117 (vs. gap), S120 (= S143), Q122 (≠ V145), D161 (= D184)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 77% coverage: 38:256/284 of query aligns to 14:235/238 of 1lafE

query
sites
1lafE

Y
|
Y

P

A

P

P

F

F

T

S

V

S

K

K

G

D

G

A

N

K

G

G

Q

E

W

F

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

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K

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L

M

C

C

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R

M

M

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A

V

E

K

C

C

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T

I

W

K

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E

A

V

S

A

D

W
x
F

D

D

G

A

I

L

I

I

P

P

S

S

L

L

L

K

A

A

K

K

I

I

D

D

V

A

I

I

F

I

S
|
S

M

L

S
|
S

V

I

T

T

D

D

E

K

R
|
R

E

Q

K

Q

Q

E

I

I

A

A

F

F

S

S

R

D

A

K

Y

L

Y

Y

D

A

S

A

L

D

L

S

G

R

V

L

A

I

G

A

P

A

K

K

G

G

S

S

E

P

V

I

E

Q

I

P

S

T

P

L

A

E

G

S

L

L

K

K

G

G

K

K

L

H

I

V

G

G

V

V

-
x
L

Q

Q

I

G

S
|
S

T
|
T

V
x
Q

S

E

A

A

N

Y

Y

A

L

N

K

D

K

N

Y

W

Y

R

E

T

N

K

I

G

A

V

D

D

L

V

K

V

Y

A

Y

Y

D

A

T

N

Q

Q

E

D

S

L

A

I

N

Y

A

S

D

D

L

L

I

T

A

A

G

G

R

R

I

L

D

D

Y

A

M

A

M

L

A

Q

D
|
D

D

E

T

V

A

A

I

A

A

S

M

E

-

G

M

F

V

L

K

K

T

Q

P

P

E

A

A

G

S

K

G

E

L

Y

A

A

H

F

I

A

A

G

-

P

S

S

V

V

P

K

Y

D

D

K

P

K

I

Y

I

F

G

G

R

D

G

G

V

T

G

G

A

V

G

G

L

L

R

R

K

K

E

D

D

D

T

T

A

E

L

L

K

K

A

A

R

A

L

F

D

D

K

K

A

A

I

L

G

T

E

E

L

L

L

R

V

Q

S

D

K

G

D

T

Y

Y

D

D

D

K

L

M

S

A

Q

K

H

K

Y

Y

F

F

G

D

L

F

S

N

V

V

binding arginine: Y14 (= Y38), F52 (≠ W76), S69 (= S93), S70 (= S94), S72 (= S96), R77 (= R101), L117 (vs. gap), S120 (= S143), T121 (= T144), Q122 (≠ V145), D161 (= D184)

Sites not aligning to the query:

binding arginine: 11

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
35% identity, 81% coverage: 28:256/284 of query aligns to 3:235/238 of 1hslA

query
sites
1hslA

P

P

I

Q

V

K

F

I

A

R

V

I

A

G

A

T

E

D

P

P

-

T

Y
|
Y

P

A

P

P

F

F

T

E

V

S

K

K

G

N

G

A

N

Q

G

G

Q

E

W

L

S

V

G

G

F

F

E

D

V

I

D

D

L

L

I

A

H

K

K

E

L

L

C

C

E

K

G

R

M

I

K

N

A

T

E

Q

C

C

Q

T

I

F

K

V

E

E

V

N

A

P

W
x
L

D

D

G

A

I

L

I

I

P

P

S

S

L

L

L

K

A

A

K

K

I

I

D

D

V

A

I

I

F

M

S
|
S

M
x
L

S
|
S

V

I

T

T

D

E

E

K

R
|
R

E

Q

K

Q

Q

E

I

I

A

A

F

F

S

T

R

D

A

K

Y

L

Y

Y

-

A

D

D

S

S

L

R

L

L

G

V

V

V

A

A

G

-

P

-

K

K

G

N

S

S

E

D

V

I

E

Q

I

P

S

T

P

V

A

A

G

S

L

L

K

K

G

G

K

K

L

R

I

V

G

G

V

V

Q

L

I

Q

S

G

T
|
T

V
x
T

S

Q

A

E

N

T

Y

F

L

G

K

N

K

E

Y

H

Y

W

E

A

N

P

I

K

A

G

-

I

D

E

L

I

K

V

Y

S

Y

Y

D

Q

T

G

Q

Q

E

D

S

N

A

I

N

Y

A

S

D

D

L

L

I

T

A

A

G

G

R

R

I

I

D

D

Y

A

M

A

M

F

A

Q

D
|
D

D

E

T

V

A

A

I

A

A

S

M

E

-

G

M

F

V

L

K

K

T

Q

P

P

E

V

A

G

S

K

G

D

L

Y

A

K

H

F

I

G

A

G

-

P

S

A

V

V

P

K

Y

D

D

E

P

K

I

L

I

F

G

G

R

V

G

G

V

T

G

G

A

M

G

G

L

L

R

R

K

K

E

E

D

D

T

N

A

E

L

L

K

R

A

E

R

A

L

L

D

N

K

K

A

A

I

F

G

A

E

E

L

M

L

R

V

A

S

D

K

G

D

T

Y

Y

D

E

D

K

L

L

S

A

Q

K

H

K

Y

Y

F

F

G

D

L

F

S

D

V

V

binding histidine: Y14 (= Y38), L52 (≠ W76), S69 (= S93), S70 (= S94), L71 (≠ M95), S72 (= S96), R77 (= R101), T120 (= T144), T121 (≠ V145), D161 (= D184)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
35% identity, 81% coverage: 28:256/284 of query aligns to 25:257/260 of P02910

query
sites
P02910

P

P

I

Q

V

K

F

I

A

R

V

I

A

G

A

T

E

D

P

P

-

T

Y

Y

P

A

P

P

F

F

T
x
E

V

S

K
|
K

G

N

G

A

N

Q

G

G

Q
x
E

W

L

S

V

G

G

F

F

E

D

V

I

D

D

L

L

I

A

H

K

K

E

L

L

C

C

E

K

G

R

M

I

K

N

A

T

E

Q

C

C

Q

T

I

F

K

V

E

E

V

N

A

P

W

L

D

D

G

A

I

L

I

I

P

P

S

S

L

L

L

K

A

A

K

K

I

I

D

D

V

A

I

I

F

M

S

S

M

L

S

S

V

I

T

T

D

E

E

K

R

R

E

Q

K

Q

Q

E

I

I

A

A

F

F

S

T

R

D

A

K

Y

L

Y

Y

-

A

D

D

S

S

L

R

L

L

G

V

V

V

A

A

G

-

P

-

K

K

G

N

S

S

E

D

V

I

E

Q

I

P

S

T

P

V

A

A

G

S

L

L

K

K

G

G

K

K

L

R

I

V

G

G

V

V

Q

L

I

Q

S

G

T

T

V

T

S

Q

A

E

N

T

Y

F

L

G

K

N

K

E

Y

H

Y

W

E

A

N

P

I

K

A

G

-

I

D

E

L

I

K

V

Y

S

Y

Y

D

Q

T

G

Q

Q

E

D

S

N

A

I

N

Y

A

S

D
|
D

L

L

I

T

A

A

G

G

R
|
R

I

I

D
|
D

Y

A

M

A

M

F

A

Q

D

D

D

E

T

V

A

A

I

A

A

S

M

E

-

G

M

F

V

L

K

K

T

Q

P

P

E

V

A

G

S

K

G

D

L

Y

A

K

H

F

I

G

A

G

-

P

S

A

V

V

P

K

Y

D

D

E

P

K

I

L

I

F

G

G

R

V

G

G

V

T

G

G

A

M

G

G

L

L

R

R

K

K

E

E

D

D

T

N

A

E

L

L

K

R

A

E

R

A

L

L

D

N

K

K

A

A

I

F

G

A

E

E

L

M

L

R

V

A

S

D

K

G

D

T

Y

Y

D

E

D

K

L

L

S

A

Q

K

H

K

Y

Y

F

F

G

D

L

F

S

D

V

V

E40 (≠ T42) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (= K44) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (≠ Q49) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (= D172) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R177) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D179) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
35% identity, 81% coverage: 28:256/284 of query aligns to 25:257/260 of P0AEU0

query
sites
P0AEU0

P

P

I

Q

V

N

F

I

A

R

V

I

A

G

A

T

E

D

P

P

-

T

Y

Y

P

A

P

P

F

F

T

E

V

S

K

K

G

N

G

S

N

Q

G

G

Q

E

W

L

S

V

G

G

F

F

E

D

V

I

D

D

L

L

I

A

H

K

K

E

L

L

C
|
C

E

K

G

R

M

I

K

N

A

T

E

Q

C
|
C

Q

T

I

F

K

V

E

E

V

N

A

P

W

L

D

D

G

A

I

L

I

I

P

P

S

S

L

L

L

K

A

A

K

K

I

I

D

D

V

A

I

I

F

M

S
|
S

M

L

S
|
S

V

I

T

T

D

E

E

K

R
|
R

E

Q

K

Q

Q

E

I

I

A

A

F

F

S

T

R

D

A

K

Y

L

Y

Y

-

A

D

D

S

S

L

R

L

L

G

V

V

V

A

A

G

-

P

-

K

K

G

N

S

S

E

D

V

I

E

Q

I

P

S

T

P

V

A

E

G

S

L

L

K

K

G

G

K

K

L

R

I

V

G

G

V

V

Q

L

I

Q

S

G

T

T

V
x
T

S

Q

A

E

N

T

Y

F

L

G

K

N

K

E

Y

H

Y

W

E

A

N

P

I

K

A

G

-

I

D

E

L

I

K

V

Y

S

Y

Y

D

Q

T

G

Q

Q

E

D

S

N

A

I

N

Y

A

S

D

D

L

L

I

T

A

A

G

G

R

R

I

I

D

D

Y

A

M

A

M

F

A

Q

D
|
D

D

E

T

V

A

A

I

A

A

S

M

E

-

G

M

F

V

L

K

K

T

Q

P

P

E

V

A

G

S

K

G

D

L

Y

A

K

H

F

I

G

A

G

-

P

S

S

V

V

P

K

Y

D

D

E

P

K

I

L

I

F

G

G

R

V

G

G

V

T

G

G

A

M

G

G

L

L

R

R

K

K

E

E

D

D

T

N

A

E

L

L

K

R

A

E

R

A

L

L

D

N

K

K

A

A

I

F

G

A

E

E

L

M

L

R

V

A

S

D

K

G

D

T

Y

Y

D

E

D

K

L

L

S

A

Q

K

H

K

Y

Y

F

F

G

D

L

F

S

D

V

V

C60 (= C62) modified: Disulfide link with 67
C67 (= C69) modified: Disulfide link with 60
S91 (= S93) binding
S92 (= S94) binding
S94 (= S96) binding
R99 (= R101) binding
T143 (≠ V145) binding
D183 (= D184) binding

Sites not aligning to the query:

1:22 signal peptide

5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 12:254/256 of 5otcA

query
sites
5otcA

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S

A

S
x
A

M

M

S
x
G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-

M

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

-

D

Q

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: Y12 (= Y38), W50 (= W76), A68 (≠ S94), G70 (≠ S96), R75 (= R101), Q138 (= Q141), T141 (= T144), S142 (≠ V145), H143 (≠ S146), S180 (≠ D184)

Sites not aligning to the query:

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: 9

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 12:254/254 of 5otaA

query
sites
5otaA

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S

A

S
x
A

M

M

S
x
G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-
x
M

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

-

D

Q

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: Y12 (= Y38), W50 (= W76), A68 (≠ S94), G70 (≠ S96), R75 (= R101), M90 (vs. gap), Q138 (= Q141), T141 (= T144), S142 (≠ V145), H143 (≠ S146), S180 (≠ D184)

Sites not aligning to the query:

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: 9

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 12:254/254 of 5ot9A

query
sites
5ot9A

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S
x
A

M

M

S

G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-
x
M

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

-

D

Q

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding Histopine: Y12 (= Y38), W50 (= W76), A67 (≠ S93), A68 (≠ S94), R75 (= R101), M90 (vs. gap), Q138 (= Q141), T141 (= T144), S142 (≠ V145), H143 (≠ S146), S180 (≠ D184)

Sites not aligning to the query:

binding Histopine: 9

4powA Structure of the pbp noct in complex with pyronopaline (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 12:254/254 of 4powA

query
sites
4powA

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S
x
A

M

M

S
x
G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-
x
M

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

-

D

Q

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: Y12 (= Y38), W50 (= W76), A67 (≠ S93), A68 (≠ S94), G70 (≠ S96), R75 (= R101), M90 (vs. gap), Q138 (= Q141), T141 (= T144), S142 (≠ V145), H143 (≠ S146), S180 (≠ D184)

Sites not aligning to the query:

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: 9

5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 13:255/255 of 5itoA

query
sites
5itoA

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S
x
A

M

M

S
x
G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-

N

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

Q

D

-

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding octopine: Y13 (= Y38), W51 (= W76), A68 (≠ S93), A69 (≠ S94), G71 (≠ S96), R76 (= R101), Q139 (= Q141), T142 (= T144), S143 (≠ V145), H144 (≠ S146), S181 (≠ D184)

Sites not aligning to the query:

binding octopine: 10

5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
34% identity, 78% coverage: 38:258/284 of query aligns to 13:255/259 of 5ovzA

query
sites
5ovzA

Y
|
Y

P

A

P

P

F

Y

T

N

V

F

K

K

G

D

G

A

N

G

G

G

Q

K

W

L

S

I

G

G

F

F

E

D

V

I

D

D

L

L

I

G

H

N

K

D

L

L

C

C

E

K

G

R

M

M

K

N

A

I

E

E

C

C

Q

K

I

F

K

V

E

E

V

Q

A

A

W
|
W

D

D

G

G

I

I

P

P

S

S

L

L

L

T

A

A

K

G

K

R

I

Y

D

D

V

A

I

I

F

M

S
x
A

M

M

S
x
G

V

I

T

Q

D

P

E

A

R
|
R

E

E

K

K

Q

V

I

I

A

A

F

F

S

S

R

R

A

P

Y

Y

Y

L

-

L

-

T

-

P

-
x
M

-

T

-

F

-

L

-

T

-

A

D

D

S

S

L

P

L

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

-

N

-

L

-

P

-

L

-

D

G

N

V

I

A

T

G

P

P

E

K

Q

G

K

S

A

E

E

V

L

E

D

I

K

S

F

P

T

A

K

G

I

L

F

K

E

G

G

K

V

L

K

I

F

G

G

V

V

Q
|
Q

I

A

S

G

T
|
T

V
x
S

S
x
H

A

E

N

A

Y

F

L

M

K

K

K

Q

Y

M

E

P

N

S

I

V

A

-

D

Q

L

I

K

S

Y

T

Y

Y

D

D

T

T

Q

I

E

D

S

N

A

V

N

V

A

M

D

D

L

L

I

K

A

A

G

G

R

R

I

I

D

D

Y

A

M

S

M

L

A

A

D
x
S

D

V

T

S

A

F

I

L

A

K

M

P

M

L

V

T

K

D

T

K

P

P

E

D

A

N

S

K

G

D

L

L

A

K

H

M

I

F

A

G

S

P

V

R

P

M

Y

T

D

G

P

G

I

L

I

F

G

G

R

K

G

G

V
|
V

G

G

A

V

G

G

L

I

R

R

K

K

E

E

D

D

T

A

A

D

L

L

K

K

A

A

R

L

L

F

D

D

K

K

A

A

I

I

G

D

E

A

L

A

L

I

V

A

S

D

K

G

D

T

Y

V

D

Q

D

K

L

L

S

S

Q

Q

H

Q

Y

W

F

F

G

G

L

Y

S

D

V

A

S

S

P

P

binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: Y13 (= Y38), W51 (= W76), A68 (≠ S93), A69 (≠ S94), G71 (≠ S96), R76 (= R101), M91 (vs. gap), Q139 (= Q141), T142 (= T144), S143 (≠ V145), H144 (≠ S146), S181 (≠ D184), V213 (= V216)

Sites not aligning to the query:

binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: 10

3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
34% identity, 79% coverage: 29:252/284 of query aligns to 4:225/225 of 3tqlA

query
sites
3tqlA

I

I

V

K

F

F

A

A

V

T

A
x
E

A

A

E

T

P

-

Y
|
Y

P

P

F

Y

T

V

V

Y

K

M

G

G

N

-

G

-

Q

Q

W

V

S

E

G

G

F

F

E

G

V

A

D

D

L

I

I

V

H

K

K

A

L

V

C

C

E

K

G

Q

M

M

K

Q

A

A

E

V

C

C

Q

T

I

I

K

S

E

N

V

Q

A

P

W
|
W

D

D

G

S

I

L

I

I

P

P

S

S

L

L

L

K

A

L

K

G

K

K

I

F

D

D

V

A

I

L

F

F

S
x
G

M

M

S
x
N

V

I

T

T

D

T

E

A

R
|
R

E

Q

K

K

Q

E

I

V

A

D

F

F

S

T

R

D

A

P

Y

Y

D

T

S

N

L

S

L

V

G
x
S

V

F

A

I

G

A

P

D

K

K

G

N

S

T

E

P

V

L

E

T

I

L

S

S

P

K

A

Q

G

G

L

L

K

K

G

G

K

K

L

I

I

I

G

G

V

V

Q

Q

I

G

S

G

T

T

V

T

S

F

A

D

N

S

Y

Y

L

L

K

Q

K

D

Y

S

Y

F

E

G

N

N

I

S

A

I

D

T

L

I

K

Q

Y

R

Y

Y

D

P

T

S

Q

E

E

E

S

D

A

A

N

L

A

M

D

D

L

L

I

T

A

S

G

G

R

R

I

V

D

D

Y

A

M

V

A

G

D

D

D

T

T

P

A

L

I

I

A

K

M

Q

M

W

V

L

K

K

T

Q

P

N

E

G

A

R

S

R

G

E

L

Y

A

V

H

L

I

I

A

G

S

K

V

P

P

V

Y

N

D

D

P

P

-

N

I

Y

I

F

G

G

R

K

G

G

V

V

G

G

A

I

G

A

L

V

R

K

K

K

E

G

D

N

T

Q

A

A

L

L

K

L

A

L

R

K

L

L

D

N

K

K

A

A

I

L

G

A

E

A

L

I

L

K

V

A

S

N

K

G

D

V

Y

Y

D

A

L

I

S

V

Q

Q

H

K

Y

Y

F

F

binding arginine: E9 (≠ A34), Y12 (= Y38), W48 (= W76), G65 (≠ S93), G66 (≠ S94), N68 (≠ S96), R73 (= R101), S89 (≠ G117)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
31% identity, 76% coverage: 38:253/284 of query aligns to 14:224/225 of 4zv2A

query
sites
4zv2A

Y
x
F

P

P

F

F

T

G

V

F

K

K

G

D

G

E

N

N

G

G

Q

K

W

L

S

V

G

G

F

F

E

D

V

I

D

D

L

L

I

A

H

K

K

A

L

I

C

A

E

K

G

K

M

L

K

G

A

V

E

K

C

V

Q

E

I

F

K

K

E

P

V

M

A

D

W
x
F

D

D

G

G

I

I

P

P

S

A

L

L

L

Q

A

S

K

G

K

K

I

I

D

D

V

V

I

V

F

I

S

A

S
x
G

M
|
M

S
x
T

V

I

T

T

D

E

E

E

R
|
R

E

K

K

K

Q

Q

I

V

A

D

F

F

S

S

R

D

A

P

Y

Y

Y

F

D

E

S

A

L

G

L

Q

G

A

V

I

A

V

G

V

P

K

K

K

G

G

S

N

E

D

V

S

E

I

I

K

S

S

P

L

A

E

G

D

L

L

K

K

G

G

K

K

L

K

I

V

G

G

V

V

Q

Q

I

L

S

G

T
x
S

V
x
T

S

S

A

E

N

Q

Y

H

L

V

K

K

Y

V

Y

A

E

A

N

G

I

V

A

K

D

-

L

V

K

K

Y

K

Y

F

D

D

T

N

Q

F

E

S

S

E

A

A

N

F

A

Q

D

E

L

L

I

K

A

S

G

G

R

R

I

V

D

D

Y

A

M

V

A

T

D
|
D

D

N

-

A

T

V

A

A

I

L

A

A

M

Y

M

V

V

K

K

Q

T

N

P

P

E

N

A

A

S

G

G

-

L

-

A

-

H

-

I

-

A

V

S

K

V

I

P

V

Y

G

D

E

P

T

I

F

I

S

G

G

R

E

G

P

V

Y

G

G

A

I

G

A

L

V

R

R

K

K

E

G

D

N

T

S

A

E

L

L

K

L

A

E

R

K

L

I

D

N

K

K

A

A

I

L

G

E

E

E

L

M

L

K

V

K

S

D

K

G

D

T

Y

Y

D

D

D

K

L

I

S

Y

Q

E

H

K

Y

W

F

F

G

G

binding glutamine: F14 (≠ Y38), F52 (≠ W76), G70 (≠ S94), M71 (= M95), T72 (≠ S96), R77 (= R101), S120 (≠ T144), T121 (≠ V145), D159 (= D184)

Sites not aligning to the query:

binding glutamine: 11

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
31% identity, 76% coverage: 38:253/284 of query aligns to 14:226/226 of 4zv1A

query
sites
4zv1A

Y
x
F

P

P

F

F

T

G

V

F

K

K

G

D

G

E

N

N

G

G

Q

K

W

L

S

V

G

G

F

F

E

D

V

I

D

D

L

L

I

A

H

K

K

A

L

I

C

A

E

K

G

K

M

L

K

G

A

V

E

K

C

V

Q

E

I

F

K

K

E

P

V

M

A

D

W
x
F

D

D

G

G

I

I

P

P

S

A

L

L

L

Q

A

S

K

G

K

K

I

I

D

D

V

V

I

V

F

I

S
x
A

S
x
G

M
|
M

S
x
T

V

I

T

T

D

E

E

E

R
|
R

E

K

K

K

Q

Q

I

V

A

D

F

F

S

S

R

D

A

P

Y

Y

Y

F

D

E

S

A

L

G

L

Q

G

A

V

I

A

V

G

V

P

K

K

K

G

G

S

N

E

D

V

S

E

I

I

K

S

S

P

L

A

E

G

D

L

L

K

K

G

G

K

K

L

K

I

V

G

G

V

V

Q
|
Q

I

L

S

G

T
x
S

V
x
T

S

S

A

E

N

Q

Y

H

L

V

K

K

Y

V

Y

A

E

K

N

D

I

A

A

G

-

V

D

K

L

V

K

K

Y

K

Y

F

D

D

T

N

Q

F

E

S

S

E

A

A

N

F

A

Q

D

E

L

L

I

K

A

S

G

G

R

R

I

V

D

D

Y

A

M

V

A

T

D
|
D

D

N

-

A

T

V

A

A

I

L

A

A

M

Y

M

V

V

K

K

Q

T

N

P

P

E

N

A

A

S

G

G

-

L

-

A

-

H

-

I

-

A

V

S

K

V

I

P

V

Y

G

D

E

P

T

I

F

I

S

G

G

R

E

G

P

V

Y

G

G

A

I

G

A

L

V

R

R

K

K

E

G

D

N

T

S

A

E

L

L

K

L

A

E

R

K

L

I

D

N

K

K

A

A

I

L

G

E

E

E

L

M

L

K

V

K

S

D

K

G

D

T

Y

Y

D

D

D

K

L

I

S

Y

Q

E

H

K

Y

W

F

F

G

G

binding arginine: F14 (≠ Y38), F52 (≠ W76), A69 (≠ S93), G70 (≠ S94), M71 (= M95), T72 (≠ S96), R77 (= R101), Q117 (= Q141), S120 (≠ T144), T121 (≠ V145), D161 (= D184)

Sites not aligning to the query:

binding arginine: 11

8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine
32% identity, 77% coverage: 38:257/284 of query aligns to 18:266/267 of 8hqrA

query
sites
8hqrA

Y
|
Y

P

P

F

W

T

N

V

S

K

K

G

D

G

A

N

S

G

G

Q

A

W

L

S

I

G

G

F

F

E

E

V

V

D

E

L

L

I

A

H

E

K

E

L

L

C

C

E

K

G

I

M

M

K

G

A

R

E

E

C

C

Q

T

I

I

K

V

E

E

V

Q

A

D

W
|
W

D

D

G

G

I

M

I

I

P

P

S

A

L

L

A

M

K

R

K

K

I

F

D

D

V

A

I

I

F

M

S
x
A

S
x
G

M

M

S
|
S

V

I

T

T

D

A

E

E

R
|
R

E

Q

K

K

Q

T

I

I

A

T

F

F

S

S

R

Q

A

G

Y

Y

Y

A

D

D

S
x
E

L

V

L

A

G

A

V

L

A

A

G

V

P

M

K

K

G

G

S

S

E

S

V

L

E

E

I

S

-

M

-

D

S

T

P

P

A

E

G

G

-

I

-

N

-

L

-

T

-

L

-

G

-

S

-

A

-

V

-

K

-

T

-

L

-

K

-

T

-

L

-

T

-

A

L

L

K

A

G

G

K

K

L

T

I

V

G

C

V

T

Q
|
Q

I

T

S

G

T
|
T

V
x
I

S

H

A

Q

N

N

Y

F

L

L

K

E

K

S

Y

G

Y

D

E

V

N

G

I

K

A

V

D

N

L

V

K

R

Y

T

Y

Y

D

K

T

T

Q

Q

E

D

S

E

A

V

N

N

A

L

D

D

L

L

I

T

A

S

G

G

R

R

I

C

D

D

Y

V

M

A

M

L

-

A

-

V

-

A

-

F

-

T

-

D

-

Y

A

A

D

D

D

K

T

S

A

G

I

K

A

P

M

V

V

L

K

V

T

G

P

P

E

T

A

F

S

S

G

G

L

G

A

A

H

-

I

-

A

-

S

-

V

-

P

-

Y

-

D

-

P

-

I

-

I

F

G

G

R

N

G

G

V

V

G

G

A

V

G

G

L

I

R

R

K

Q

-

G

-

D

-

A

-

I

-

G

-

T

E

R

D

D

T

A

A

K

L

L

K

L

A

K

R

D

L

F

D

N

K

K

A

A

I

I

G

N

E

T

L

A

L

R

V

K

S

Q

K

G

D

I

Y

I

D

S

D

K

L

L

S

A

Q

I

H

K

Y

H

F

F

G

G

L

F

S

D

V

A

S

S

binding arginine: Y18 (= Y38), W56 (= W76), A73 (≠ S93), G74 (≠ S94), S76 (= S96), R81 (= R101), E94 (≠ S114), Q143 (= Q141), T146 (= T144), I147 (≠ V145)

Sites not aligning to the query:

binding arginine: 15

Query Sequence

>GFF2658 FitnessBrowser__WCS417:GFF2658
MKSKRMATWLGSAVLMLAAGFSWAAEKPIVFAVAAEPYPPFTVKGGNGQWSGFEVDLIHK
LCEGMKAECQIKEVAWDGIIPSLLAKKIDVIFSSMSVTDEREKQIAFSRAYYDSLLGVAG
PKGSEVEISPAGLKGKLIGVQISTVSANYLKKYYENIADLKYYDTQESANADLIAGRIDY
MMADDTAIAMMVKTPEASGLAHIASVPYDPIIGRGVGAGLRKEDTALKARLDKAIGELLV
SKDYDDLSQHYFGLSVSPCKRTDTPAFVSKTCDSPYPQIAQKTE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory