SitesBLAST
Comparing GFF2666 FitnessBrowser__Marino:GFF2666 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 95% coverage: 13:417/427 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L16), G8 (= G17), G10 (= G19), V11 (= V20), I12 (≠ V21), V30 (= V39), E31 (≠ D40), K32 (≠ R41), E38 (= E48), A39 (≠ T49), S40 (= S50), A43 (≠ N53), G45 (= G55), L46 (≠ Q56), V171 (≠ I234), G200 (≠ M267), G201 (= G268), W203 (≠ F270), G298 (= G368), R300 (= R370), P301 (= P371), Y326 (≠ G395), R327 (≠ T396), N328 (≠ L397), G329 (= G398), I330 (≠ W399)
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 69% coverage: 130:422/427 of query aligns to 77:360/368 of 4yshB
- active site: I262 (≠ V325), L283 (≠ I346), G305 (= G368), N335 (≠ L397), L338 (≠ T400)
- binding flavin-adenine dinucleotide: V178 (≠ I234), S206 (≠ M267), W209 (≠ F270), R307 (= R370), H332 (= H394), R334 (≠ T396), N335 (≠ L397), G336 (= G398), I337 (≠ W399), L338 (≠ T400)
- binding glycine: G249 (≠ D310), Y251 (= Y312), Y251 (= Y312), A264 (≠ G327), R307 (= R370), R334 (≠ T396), R334 (≠ T396)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 69% coverage: 130:422/427 of query aligns to 77:360/370 of 4yshA
- active site: I262 (≠ V325), L283 (≠ I346), G305 (= G368), N335 (≠ L397), L338 (≠ T400)
- binding flavin-adenine dinucleotide: V178 (≠ I234), S206 (≠ M267), G207 (= G268), W209 (≠ F270), R307 (= R370), H332 (= H394), R334 (≠ T396), N335 (≠ L397), G336 (= G398), I337 (≠ W399)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
24% identity, 69% coverage: 130:422/427 of query aligns to 78:362/377 of Q5L2C2
- V180 (≠ I234) binding
- R309 (= R370) binding
- 334:340 (vs. 394:400, 29% identical) binding
- R336 (≠ T396) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
23% identity, 67% coverage: 138:425/427 of query aligns to 94:383/403 of 2gagB
- binding flavin-adenine dinucleotide: V195 (≠ I234), G224 (≠ M267), A225 (≠ G268), H227 (≠ F270), L231 (= L274), L246 (≠ I292), G352 (= G395), T353 (= T396), G354 (≠ L397), G355 (= G398), F356 (≠ W399), K357 (≠ T400)
- binding flavin mononucleotide: H171 (≠ A210), V250 (= V296), E278 (≠ D321), R321 (≠ E364), W323 (= W366)
- binding 2-furoic acid: M263 (≠ D309), Y270 (= Y316), K357 (≠ T400)
- binding sulfite ion: K170 (≠ D209), K276 (≠ L319)
Sites not aligning to the query:
- active site: 61, 64, 65
- binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52, 58, 59, 60, 62, 63, 64, 66
- binding flavin mononucleotide: 61, 62
- binding 2-furoic acid: 64, 66, 68, 401
P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
22% identity, 68% coverage: 125:413/427 of query aligns to 85:372/405 of P40875
- C146 (≠ E186) mutation to S: No change in activity.
- H173 (≠ A210) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
- H175 (≠ M212) mutation to A: No effect on FMN binding and activity.
- C195 (≠ T232) mutation to S: No change in activity.
- C351 (≠ T392) mutation to A: No change in activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.
1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
24% identity, 58% coverage: 171:418/427 of query aligns to 130:376/402 of 1vrqB
- active site: G326 (= G368), K358 (≠ T400)
- binding n,n-dimethylglycine: K358 (≠ T400)
- binding flavin-adenine dinucleotide: V196 (≠ I234), A224 (≠ S266), G225 (≠ M267), H228 (≠ F270), L247 (≠ I292), G353 (= G395), T354 (= T396), G355 (≠ L397), G356 (= G398), F357 (≠ W399), K358 (≠ T400)
- binding flavin mononucleotide: H172 (≠ A210), V251 (= V296), E279 (≠ D321), R322 (≠ E364), W324 (= W366)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding n,n-dimethylglycine: 65, 67, 69, 401
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
24% identity, 58% coverage: 171:419/427 of query aligns to 130:377/404 of 3ad8B
- active site: G326 (= G368), K358 (≠ T400)
- binding flavin-adenine dinucleotide: V196 (≠ I234), G225 (≠ M267), A226 (≠ G268), H228 (≠ F270), L247 (≠ I292), G353 (= G395), T354 (= T396), G355 (≠ L397), G356 (= G398), F357 (≠ W399), K358 (≠ T400)
- binding flavin mononucleotide: H172 (≠ A210), V251 (= V296), E279 (≠ D321), R322 (≠ E364), W324 (= W366)
- binding pyrrole-2-carboxylate: M264 (≠ D309), Y271 (= Y316), T354 (= T396), K358 (≠ T400)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding pyrrole-2-carboxylate: 65, 67, 69, 402
3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
24% identity, 58% coverage: 171:419/427 of query aligns to 130:377/404 of 3ad7B
- active site: G326 (= G368), K358 (≠ T400)
- binding flavin-adenine dinucleotide: V196 (≠ I234), G225 (≠ M267), A226 (≠ G268), H228 (≠ F270), L247 (≠ I292), G353 (= G395), T354 (= T396), G355 (≠ L397), G356 (= G398), F357 (≠ W399), K358 (≠ T400)
- binding flavin mononucleotide: H172 (≠ A210), V251 (= V296), K277 (≠ L319), E279 (≠ D321), R322 (≠ E364), W324 (= W366)
- binding [methylthio]acetate: M264 (≠ D309), Y271 (= Y316), T354 (= T396), K358 (≠ T400)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding [methylthio]acetate: 67, 69, 402
Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
24% identity, 58% coverage: 171:419/427 of query aligns to 131:378/405 of Q50LF2
- K172 (≠ D209) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
- H173 (≠ A210) modified: Tele-8alpha-FMN histidine
- V197 (≠ I234) binding
- H270 (≠ L314) mutation to A: 10-fold decrease in catalytic efficiency.
- Y272 (= Y316) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
- G354 (= G395) binding
- G357 (= G398) binding
- K359 (≠ T400) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.
Sites not aligning to the query:
- 31 binding
- 32 binding
- 53 binding
- 61 binding
- 62 binding
- 66 binding
- 68 binding
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 53% coverage: 196:422/427 of query aligns to 136:355/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ K313), R302 (= R370), R329 (≠ T396)
- binding flavin-adenine dinucleotide: V174 (≠ I234), S202 (≠ M267), G203 (= G268), W205 (≠ F270), F209 (≠ L274), G300 (= G368), R302 (= R370), H327 (= H394), R329 (≠ T396), N330 (≠ L397), G331 (= G398), I332 (≠ W399)
- binding phosphate ion: R254 (= R322)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 53% coverage: 196:422/427 of query aligns to 136:355/369 of O31616
- V174 (≠ I234) binding
- H244 (≠ E311) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R370) binding
- 327:333 (vs. 394:400, 29% identical) binding
- R329 (≠ T396) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 53% coverage: 196:422/427 of query aligns to 136:355/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ E233), V174 (≠ I234), S202 (≠ M267), G203 (= G268), W205 (≠ F270), F209 (≠ L274), G300 (= G368), R302 (= R370), H327 (= H394), F328 (≠ G395), R329 (≠ T396), N330 (≠ L397), G331 (= G398), I332 (≠ W399)
- binding glycolic acid: Y246 (≠ K313), R302 (= R370), R329 (≠ T396)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
23% identity, 96% coverage: 13:422/427 of query aligns to 4:382/827 of 3gsiA
- active site: H222 (≠ K288), Y256 (≠ V315)
- binding flavin-adenine dinucleotide: G10 (= G19), I11 (≠ V20), V12 (= V21), D32 (= D40), Q33 (≠ R41), G41 (≠ L47), S42 (≠ E48), T43 (= T49), H45 (≠ Y51), P47 (≠ N53), L49 (≠ G55), T170 (≠ E233), V171 (≠ I234), A200 (≠ M267), G201 (= G268), W203 (≠ F270), H222 (≠ K288), Y256 (≠ V315), G330 (= G368), I331 (≠ L369), F332 (≠ R370), V357 (≠ H394), W358 (= W399), V359 (vs. gap), T360 (= T400)
- binding magnesium ion: D254 (≠ K313)
Sites not aligning to the query:
- active site: 549
- binding magnesium ion: 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
23% identity, 96% coverage: 13:422/427 of query aligns to 4:382/827 of 1pj7A
- active site: H222 (≠ K288), Y256 (≠ V315)
- binding flavin-adenine dinucleotide: G8 (= G17), G10 (= G19), I11 (≠ V20), V12 (= V21), D32 (= D40), Q33 (≠ R41), G41 (≠ L47), S42 (≠ E48), T43 (= T49), H45 (≠ Y51), P47 (≠ N53), L49 (≠ G55), T170 (≠ E233), V171 (≠ I234), A200 (≠ M267), G201 (= G268), W203 (≠ F270), H222 (≠ K288), Y256 (≠ V315), I331 (≠ L369), V357 (≠ H394), W358 (= W399), V359 (vs. gap), T360 (= T400)
Sites not aligning to the query:
- active site: 549
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
23% identity, 96% coverage: 13:422/427 of query aligns to 7:385/830 of Q9AGP8
- IV 14:15 (≠ VV 20:21) binding
- DQ 35:36 (≠ DR 40:41) binding
- STSH 45:48 (≠ ETSY 48:51) binding
- L52 (≠ G55) binding
- V174 (≠ I234) binding
- H225 (≠ K288) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- Y259 (≠ V315) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- V----WVT 360:363 (≠ HGTLGW-T 394:400) binding
Sites not aligning to the query:
- 539 binding
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
23% identity, 96% coverage: 13:422/427 of query aligns to 5:383/828 of 1pj6A
- active site: H223 (≠ K288), Y257 (≠ V315)
- binding flavin-adenine dinucleotide: G9 (= G17), G11 (= G19), I12 (≠ V20), V13 (= V21), D33 (= D40), Q34 (≠ R41), G42 (≠ L47), S43 (≠ E48), T44 (= T49), H46 (≠ Y51), P48 (≠ N53), L50 (≠ G55), V172 (≠ I234), A201 (≠ M267), G202 (= G268), W204 (≠ F270), H223 (≠ K288), Y257 (≠ V315), G331 (= G368), I332 (≠ L369), V358 (≠ H394), W359 (= W399), V360 (vs. gap), T361 (= T400)
Sites not aligning to the query:
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
24% identity, 67% coverage: 132:415/427 of query aligns to 80:348/369 of S5FMM4
- K81 (≠ R133) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- S202 (≠ M267) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ W399) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L409) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
6j39A Crystal structure of cmis2 with inhibitor (see paper)
26% identity, 61% coverage: 167:425/427 of query aligns to 111:364/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: R243 (≠ V305), Y252 (≠ L314), Y267 (≠ A329), R308 (= R370), R334 (≠ T396), I335 (≠ L397)
- binding flavin-adenine dinucleotide: A174 (≠ E238), A203 (≠ M267), W206 (≠ F270), I228 (≠ S291), Y252 (≠ L314), R308 (= R370), S333 (≠ G395), R334 (≠ T396), I335 (≠ L397), G336 (= G398), V337 (≠ W399), Q338 (≠ T400)
Sites not aligning to the query:
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46, 49
- binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44
6j38A Crystal structure of cmis2 (see paper)
26% identity, 61% coverage: 167:425/427 of query aligns to 111:364/368 of 6j38A
- binding flavin-adenine dinucleotide: A174 (≠ E238), A203 (≠ M267), W206 (≠ F270), G226 (= G289), G306 (= G368), R308 (= R370), S333 (≠ G395), R334 (≠ T396), I335 (≠ L397), G336 (= G398), V337 (≠ W399), Q338 (≠ T400)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44
Query Sequence
>GFF2666 FitnessBrowser__Marino:GFF2666
MADSVRPEASMHILVLGAGVVGTTTAWYLQKQGHQVTVVDRQNQAGLETSYANGGQISVS
HAEPWANPSAPLKVMKWLFQPDAPLLFRPRLDPAQWRWALSFLGQCTLARAAHNIRQMVN
LGTYSCSQLQALREEAGIEYNHLEKGILHFYTNPAEFDGAMEPTRIMQDLGCDRQIIDAD
RAVELEPALKPIRNRIAGATYTSEDESGDARMFTQNLAKRCAEAGVEFRYGTEILSFERA
GERVLGIQTLRDGHHETLRADAYVLSMGSFSAALASQLGIFLNIYPAKGYSITVPVKNEE
AAFNVSLTDDEYKLVYSRLGDRMRVAGTAELNGYSRKLNYTRCRAIVRRTAEVMPEAGYW
DQAEFWTGLRPATPSNVPYIGKSHFANLYLNTGHGTLGWTHSCGSAAALADIIDGRKPEV
DFTFSGL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory