SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2678 FitnessBrowser__Phaeo:GFF2678 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q59104 4-hydroxybutyrate dehydrogenase; 4HbD; Gamma-hydroxybutyrate dehydrogenase; GHBDH; EC 1.1.1.61 from Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha) (see paper)
39% identity, 98% coverage: 9:381/381 of query aligns to 6:382/382 of Q59104

query
sites
Q59104

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D193 (= D200) mutation to A: Retains very low activity.
H197 (= H204) mutation to A: Loss of activity.
H261 (= H268) mutation to A: Loss of activity.
H265 (= H272) mutation to A: 75% decrease in Vmax. Optimum pH is 9.5.; mutation to C: 95% decrease in Vmax. Optimum pH is 8.5.; mutation to D: Retains very low activity.; mutation to Y: Loss of activity.
H280 (= H282) mutation to A: Retains very low activity.

P31005 NAD-dependent methanol dehydrogenase; MDH; MEDH; Type 3 alcohol dehydrogenase; EC 1.1.1.244 from Bacillus methanolicus (see 3 papers)
34% identity, 99% coverage: 6:381/381 of query aligns to 3:381/381 of P31005

query
sites
P31005

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G13 (= G16) mutation to A: Shows a reduced dehydrogenase activity.
G15 (= G18) mutation to A: Shows almost the same dehydrogenase activity as the wild-type.
D88 (= D91) mutation to N: Shows almost the same dehydrogenase activity as the wild-type.
G95 (= G98) mutation to A: Shows a 10-fold decreased affinity for NAD and NADH and a strongly reduced dehydrogenase activity. Completely insensitive to the stimulating effect of the activator protein Act.
S97 (= S100) mutation to G: Shows an increase of the dehydrogenase activity and a decrease of the affinity for NAD and NADH. Completely insensitive to the stimulating effect of the activator protein Act. It does not bind NAD.; mutation to T: Shows an increase of the dehydrogenase activity and affinity for NAD and NADH.
D100 (= D103) mutation to N: Loss of dehydrogenase activity. It still binds NADH.
K103 (= K106) mutation to R: Loss of dehydrogenase activity. It does not bind NADH.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3bfjA Crystal structure analysis of 1,3-propanediol oxidoreductase (see paper)
34% identity, 99% coverage: 6:381/381 of query aligns to 4:382/382 of 3bfjA

query
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3bfjA

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binding fe (ii) ion: D193 (= D200), H197 (= H204), H262 (= H268), H276 (= H282)

3ox4A Structures of iron-dependent alcohol dehydrogenase 2 from zymomonas mobilis zm4 complexed with NAD cofactor (see paper)
35% identity, 98% coverage: 7:380/381 of query aligns to 5:381/382 of 3ox4A

query
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3ox4A

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Y

L

K

K

A

D

G

N

D

N

H

S

D

D

G

F

V

V

I

I

A

S

F

L

G

G

G
|
G

S
|
S

G

P

L

H

D

D

L

C

G

A

K

K

M

A

V

I

A

A

F

L

M

V

C

A

G

T

Q

N

N

G

R

G

P

E

V

V

W

K

D

D

F

Y

E

E

D

G

I

I

G

D

D

K

W

S

W

K

T

K

R

P

A

A

D

-

A

-

D

-

A

-

I

-

A

L

P

P

I

L

I

M

A

S

V

I

P

N

T
|
T

A

A

G

G

T
|
T

G

A

S

S

E

E

V

M

G

T

R

R

A
x
F

S

C

V
x
I

I

I

T

T

D

D

S

E

V

V

T

R

H

H

Q

V

K

K

K

M

I

A

I

I

F

V

H

D

P

R

K

H

V

V

L

T

P

P

T

M

V

V

V

S

I

V

C

N

D

D

P

P

E

L

L
|
L

T

M

V

V

G
|
G

M
|
M

P

P

K

K

F

G

I
x
L

T

T

A

A

G

A

T

T

G

G

L

M

D
|
D

A

A

F

L

A

T

H
|
H

C

A

V

F

E

E

A

A

F

Y

S

S

P

T

H

A

Y

A

H

T

P

P

M

I

S

T

Q

D

G

A

I

C

A

A

L

L

E

K

G

A

M

A

R

S

L

M

V

I

K

A

E

K

Y

N

L

L

P

K

R

T

A

A

Y

C

A

D

D

N

G

G

T

K

D

D

I

M

E

P

A

A

R

R

A

E

Q

A

M

M

M

A

S

Y

A

A

A

Q

A

F

M

L

G

A

A

G

T

M

A

A

F

F

Q

N

K

N

-

A

G

S

L

L

G

G

A

Y

I

V

H
|
H

A

A

M

M

S

A

H
|
H

P

Q

I

L

G

G

A

G

L

Y

F

Y

N

N

T

L

H

P

H
|
H

G

G

T

V

T

C

N

N

A

A

V

V

C

L

M

L

L

P

A

H

V

V

L

L

A

A

F

Y

N

N

A

A

P

S

E

V

I

V

A

A

D

G

R

R

F

L

K

K

T

D

A

V

A

G

D

V

Y

A

L

M

G

G

I

L

D

D

-

I

-

A

-

N

-

L

G

G

G

D

F

K

E

E

G

G

V

A

C

E

A

A

Y

T

V

I

Q

Q

E

A

L

V

N

R

D

D

-

L

-

A

S

S

L

I

G

G

I

I

P

P

R

A

G

N

L

L

S

T

E

E

L

L

G

G

V

A

T

K

E

K

A

E

A

D

I

V

P

P

E

L

L

L

V

A

K

D

G

H

A

A

L

L

I

K

D

D

P

A

S

C

C

A

G

L

G

T

N

N

P

P

V

R

V

Q

L

G

D

D

Q

Q

A

K

N

E

L

V

T

E

Q

E

L

L

F

F

K

L

D

S

A

A

binding fe (ii) ion: D193 (= D200), H197 (= H204), H262 (= H268), H276 (= H282)
binding nicotinamide-adenine-dinucleotide: D38 (= D40), F40 (≠ G42), M41 (≠ L43), N70 (= N72), G96 (= G98), G97 (= G99), S98 (= S100), T137 (= T144), T138 (= T145), T141 (= T148), F148 (≠ A155), I150 (≠ V157), L178 (= L185), G181 (= G188), M182 (= M189), L186 (≠ I193), H197 (= H204), H266 (= H272), H276 (= H282)

3owoA Structures of iron-dependent alcohol dehydrogenase 2 from zymomonas mobilis zm4 with and without NAD cofactor (see paper)
35% identity, 98% coverage: 7:380/381 of query aligns to 5:381/382 of 3owoA

query
sites
3owoA

W

F

S

Y

Y

I

P

P

T

F

A

V

I

N

K

E

F

M

G

G

A

E

G

G

R

S

I

L

R

E

E

K

L

A

A

I

E

K

A

D

C

L

A

N

T

G

A

S

G

G

M

F

K

K

K

N

P

A

L

L

L

I

V

V

T

S

D

D

K

A

G

F

L

M

A

N

D

K

L

S

P

G

V

V

T

V

Q

K

S

Q

T

V

L

A

D

D

I

L

L

L

E

K

A

A

A

Q

G

G

L

I

G

N

R

S

A

A

M

V

F

Y

C

D

E

G

V

V

D

M

P

P

N

N

P

P

N

T

E

V

K

T

N

A

L

V

E

L

A

E

G

G

V

L

A

K

A

I

Y

L

K

K

A

D

G

N

D

N

H

S

D

D

G

F

V

V

I

I

A

S

F

L

G

G

S

S

G

P

L

H

D

D

L

C

G

A

K

K

M

A

V

I

A

A

F

L

M

V

C

A

G

T

Q

N

N

G

R

G

P

E

V

V

W

K

D

D

F

Y

E

E

D

G

I

I

G

D

D

K

W

S

W

K

T

K

R

P

A

A

D

-

A

-

D

-

A

-

I

-

A

L

P

P

I

L

I

M

A

S

V

I

P

N

T

T

A

A

G

G

T

T

G

A

S

S

E

E

V

M

G

T

R

R

A

F

S

C

V

I

I

I

T

T

D

D

S

E

V

V

T

R

H

H

Q

V

K

K

K

M

I

A

I

I

F

V

H

D

P

R

K

H

V

V

L

T

P

P

T

M

V

V

V

S

I

V

C

N

D

D

P

P

E

L

L

L

T

M

V

V

G

G

M

M

P

P

K

K

F

G

I

L

T

T

A

A

G

A

T

T

G

G

L

M

D
|
D

A

A

F

L

A

T

H
|
H

C

A

V

F

E

E

A

A

F

Y

S

S

P

T

H

A

Y

A

H

T

P

P

M

I

S

T

Q

D

G

A

I

C

A

A

L

L

E

K

G

A

M

A

R

S

L

M

V

I

K

A

E

K

Y

N

L

L

P

K

R

T

A

A

Y

C

A

D

D

N

G

G

T

K

D

D

I

M

E

P

A

A

R

R

A

E

Q

A

M

M

M

A

S

Y

A

A

A

Q

A

F

M

L

G

A

A

G

T

M

A

A

F

F

Q

N

K

N

-

A

G

S

L

L

G

G

A

Y

I

V

H
|
H

A

A

M

M

S

A

H

H

P

Q

I

L

G

G

A

G

L

Y

F

Y

N

N

T

L

H

P

H
|
H

G

G

T

V

T

C

N

N

A

A

V

V

C

L

M

L

L

P

A

H

V

V

L

L

A

A

F

Y

N

N

A

A

P

S

E

V

I

V

A

A

D

G

R

R

F

L

K

K

T

D

A

V

A

G

D

V

Y

A

L

M

G

G

I

L

D

D

-

I

-

A

-

N

-

L

G

G

G

D

F

K

E

E

G

G

V

A

C

E

A

A

Y

T

V

I

Q

Q

E

A

L

V

N

R

D

D

-

L

-

A

S

S

L

I

G

G

I

I

P

P

R

A

G

N

L

L

S

T

E

E

L

L

G

G

V

A

T

K

E

K

A

E

A

D

I

V

P

P

E

L

L

L

V

A

K

D

G

H

A

A

L

L

I

K

D

D

P

A

S

C

C

A

G

L

G

T

N

N

P

P

V

R

V

Q

L

G

D

D

Q

Q

A

K

N

E

L

V

T

E

Q

E

L

L

F

F

K

L

D

S

A

A

binding fe (ii) ion: D193 (= D200), H197 (= H204), H262 (= H268), H276 (= H282)

6scgA Structure of adhe form 1 (see paper)
33% identity, 97% coverage: 10:379/381 of query aligns to 8:397/406 of 6scgA

query
sites
6scgA

P

P

T

K

A

S

I

I

K

Y

F

F

G

R

A

R

G

G

R

S

I

L

R

P

E

I

L

A

A

L

E

D

A

E

C

V

A

I

T

T

A

D

G

G

M

H

K

K

K

R

P

A

L

L

L

I

V

V

T

T

D
|
D

K

R

G
x
F

L
|
L

A

F

D

N

L

N

P

G

V

Y

T

A

Q

D

S

Q

T

I

L

T

D

S

I

V

L

L

E

K

A

A

G

G

L

V

G

E

R

T

A

E

M

V

F

F

C

F

E

E

V

V

D

E

P
x
A

N
x
D

P

P

N

T

E

L

K

S

N

I

L

V

E

R

A

K

G

G

V

A

A

E

A

L

Y

A

K

N

A

S

G

F

D

K

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

G
|
G

S
|
S

G

P

L

M

D
|
D

L

A

G

A

K

K

M

I

V

M

A

W

F

V

M

M

C

-

G

-

Q

Y

N

E

R

H

P

P

V

E

W

T

D

H

F

F

E

E

D

E

I

L

G

A

-

L

-

R

-

F

-

M

D

D

W

I

W

R

T

K

R

R

A

I

-

Y

-

K

-

F

-

P

D

K

A

M

D

G

A

V

I

K

A

A

P

K

I

M

I

I

A

A

V

V

P

T

T
|
T

A

S

G

G

T
|
T

G

G

S

S

E

E

V

V

G

T

R

P

A

F

S

A

V
|
V

I

V

T

T

D

D

S

D

V

A

T

T

H

G

Q

Q

K

K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

A

V

L

L

T

P

P

T

D

V

M

V

A

I

I

C

V

D

D

P

A

E

N

L
|
L

T

V

V

M

G

D

M
|
M

P
|
P

K

K

F

S

I
x
L

T

C

A

A

G

F

T

G

G

G

L

L

D
|
D

A

A

F

V

A

T

H
|
H

C

A

V

M

E

E

A

A

F

Y

S

V

S

S

P

V

H

L

Y

A

H

S

P

E

M

F

S

S

Q

D

G

G

I

Q

A

A

L

L

E

Q

G

A

M

L

R

K

L

L

V

L

K

K

E

E

Y

Y

L

L

P

P

R

A

A

S

Y

Y

A

H

D

E

G

G

T

S

-

K

D

N

I

P

E

V

A

A

R

R

A

E

Q

R

M

V

M

H

S

S

A

A

A

T

M

I

G

A

A

G

T

I

A

A

F

F

Q

A

K

N

G

A

-

F

L

L

G

G

A

V

I

C

H
|
H

A

S

M

M

S

A

H
|
H

P

K

I

L

G

G

A

S

L

Q

F

F

N

H

T

I

H

P

H
|
H

G

G

T

L

T

A

N

N

A

A

V

L

C

L

M

I

L

C

A

N

V

V

L

I

A

R

F

Y

N

N

A

A

-

N

P

P

E

Q

I

A

A

R

D

R

R

R

F

Y

K

A

T

E

A

I

A

A

D

D

Y

H

L

L

G

G

I

L

D

S

-

A

-

P

-

G

-

D

-

R

-

T

-

A

G

A

G

K

F

I

E

E

G

K

V

L

C

L

A

A

Y

W

V

L

Q

E

E

T

L

L

N

K

D

A

S

E

L

L

G

G

I

I

P

P

R

K

G

S

L

I

S

R

E

E

L

A

G

G

V

V

T

Q

E

E

-

A

-

D

-

F

-

L

A

A

A

N

I

V

P

D

E

K

L

L

V

S

K

E

G

D

A

A

L

F

I

D

D

D

P

Q

S

C

C

T

G

G

G

A

N

N

P

P

V

R

V

Y

L

P

D

L

Q

I

A

S

N

E

L

L

T

K

Q

Q

L

I

F

L

K

L

D

D

binding fe (iii) ion: D204 (= D200), H208 (= H204), H274 (= H268), H288 (= H282)
binding nicotinamide-adenine-dinucleotide: D38 (= D40), F40 (≠ G42), L41 (= L43), A69 (≠ P71), D70 (≠ N72), G96 (= G98), G97 (= G99), S98 (= S100), D101 (= D103), T148 (= T144), T149 (= T145), T152 (= T148), V161 (= V157), L189 (= L185), M193 (= M189), P194 (= P190), L197 (≠ I193), H278 (= H272)

P0A9Q7 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli (strain K12) (see 8 papers)
32% identity, 97% coverage: 10:379/381 of query aligns to 457:860/891 of P0A9Q7

query
sites
P0A9Q7

P

P

T

K

A

S

I

I

K

Y

F

F

G

R

A

R

G

G

R

S

I

L

R

P

E

I

L

A

A

L

E

D

A

E

C

V

A

I

T

T

A

D

G

G

M

H

K

K

K

R

P

A

L

L

L

I

V

V

T

T

D
|
D

K

R

G

F

L

L

A

F

D

N

L

N

P

G

V

Y

T

A

Q

D

S

Q

T

I

L

T

D

S

I

V

L

L

E

K

A

A

G

G

L

V

G

E

R

T

A

E

M

V

F

F

C

F

E

E

V

V

D

E

P

A

N
x
D

P

P

N

T

E

L

K

S

N

I

L

V

E

R

A

K

G

G

V

A

A

E

A

L

Y

A

K

N

A

S

G

F

D

K

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

G
|
G

S
|
S

G
x
P

L
x
M

D
|
D

L

A

G

A

K

K

M

I

V

M

A

W

F

V

M

M

C

Y

G

-

Q

-

N

E

R

H

P

P

V

E

W

T

D

H

F

F

E

E

D
x
E

I

L

G

A

-

L

-

R

-

F

-

M

D

D

W

I

W

R

T

K

R

R

A

I

-

Y

-

K

-

F

-

P

D

K

A

M

D

G

A

V

I

K

A

A

P

K

I

M

I

I

A

A

V

V

P

T

T

T

A

S

G

G

T

T

G

G

S

S

E

E

V

V

G

T

R

P

A

F

S

A

V
|
V

I

V

T

T

D

D

S

D

V

A

T

T

H

G

Q

Q

K
|
K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

A

V

L

L

T

P

P

T

D

V

M

V

A

I

I

C

V

D

D

P

A

E

N

L

L

T

V

V

M

G

D

M

M

P

P

K

K

F

S

I

L

T

C

A

A

G

F

T

G

G

G

L

L

D
|
D

A

A

F

V

A

T

H
|
H

C

A

V

M

E

E

A

A

F

Y

S

V

S

S

P

V

H

L

Y

A

H

S

P

E

M
x
F

S

S

Q

D

G

G

I

Q

A

A

L

L

E

Q

G

A

M

L

R

K

L

L

V

L

K

K

E

E

Y

Y

L

L

P

P

R

A

A

S

Y

Y

A

H

D

E

G

G

T

S

-

K

D

N

I

P

E

V

A

A

R

R

A

E

Q

R

M

V

M

H

S

S

A

A

A

T

M

I

G

A

A

G

T

I

A

A

F

F

Q

A

K

N

G

A

-

F

L

L

G

G

A

V

I

C

H
|
H

A

S

M

M

S

A

H

H

P

K

I

L

G

G

A

S

L

Q

F

F

N

H

T

I

H

P

H
|
H

G

G

T

L

T

A

N

N

A

A

V

L

C

L

M

I

L

C

A

N

V

V

L

I

A

R

F

Y

N

N

A

A

-

N

-

D

-

N

-

P

-

T

-

K

-

Q

-

T

-

A

-

F

-

S

-

Q

-

Y

-

D

-

R

P

P

E

Q

I

A

A

R

D

R

R

R

F

Y

K

A

T

E

A

I

A

A

D

D

Y

H

L

L

G

G

I

L

D

S

-

A

-

P

-

G

-

D

-

R

-

T

-

A

G

A

G

K

F

I

E

E

G

K

V

L

C

L

A

A

Y

W

V

L

Q

E

E

T

L

L

N

K

D

A

S

E

L

L

G

G

I

I

P

P

R

K

G

S

L

I

S

R

E

E

L

A

G

G

V

V

T

Q

E

E

-

A

-

D

-

F

-

L

A

A

A

N

I

V

P

D

E

K

L

L

V

S

K

E

G

D

A

A

L

F

I

D

D

D

P

Q

S

C

C

T

G

G

G

A

N

N

P

P

V

R

V

Y

L

P

D

L

Q

I

A

S

N

E

L

L

T

K

Q

Q

L

I

F

L

K

L

D

D

D487 (= D40) binding
D519 (≠ N72) binding
GSPMD 546:550 (≠ GSGLD 99:103) binding
E568 (≠ D123) mutation to K: Partially restores protein stability and resistance to MCO damage; when associated with T-267.
V610 (= V157) binding
K619 (= K166) binding
D653 (= D200) binding
H657 (= H204) binding
F670 (≠ M217) mutation F->A,E,V: Disrupts spirosome formation. Affects the forward activity of ALDH.
H723 (= H268) binding
H737 (= H282) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
110:115 binding
195 binding
213 binding
267 A→T: Shows aerobic growth ability on ethanol. Shows 5-6 fold increase in acetaldehyde dehydrogenase activity, but does not affect ethanol dehydrogenase activity. Shows decreased thermal enzyme stability and increased sensitivity to MCO damage. Shows increased protein stability and resistance to MCO; when associated with K-568.
335 binding
358 modified: N6-acetyllysine
419 binding
446:449 mutation Missing: Can form dimers, but does not assemble into long filaments. Strongly affects ALDH activity, but not ADH activity.

P0A9Q8 Bifunctional aldehyde-alcohol dehydrogenase AdhE; Alcohol dehydrogenase E; EC 1.2.1.10; EC 1.1.1.1 from Escherichia coli O157:H7 (see paper)
32% identity, 97% coverage: 10:379/381 of query aligns to 457:860/891 of P0A9Q8

query
sites
P0A9Q8

P

P

T

K

A

S

I

I

K

Y

F

F

G

R

A

R

G

G

R

S

I

L

R

P

E

I

L

A

A

L

E

D

A

E

C

V

A

I

T

T

A

D

G

G

M

H

K

K

K

R

P

A

L

L

L

I

V

V

T

T

D
|
D

K

R

G

F

L

L

A

F

D

N

L

N

P

G

V

Y

T

A

Q

D

S

Q

T

I

L

T

D

S

I

V

L

L

E

K

A

A

G

G

L

V

G

E

R

T

A

E

M

V

F

F

C

F

E

E

V

V

D

E

P

A

N
x
D

P

P

N

T

E

L

K

S

N

I

L

V

E

R

A

K

G

G

V

A

A

E

A

L

Y

A

K

N

A

S

G

F

D

K

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

G
|
G

S
|
S

G
x
P

L
x
M

D
|
D

L

A

G

A

K

K

M

I

V

M

A

W

F

V

M

M

C

Y

G

-

Q

-

N

E

R

H

P

P

V

E

W

T

D

H

F

F

E

E

D

E

I

L

G

A

-

L

-

R

-

F

-

M

D

D

W

I

W

R

T

K

R

R

A

I

-

Y

-

K

-

F

-

P

D

K

A

M

D

G

A

V

I

K

A

A

P

K

I

M

I

I

A

A

V

V

P

T

T
|
T

A

S

G

G

T

T

G

G

S

S

E

E

V

V

G

T

R

P

A

F

S

A

V

V

I

V

T

T

D

D

S

D

V

A

T

T

H

G

Q

Q

K

K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

A

V

L

L

T

P

P

T

D

V

M

V

A

I

I

C

V

D

D

P

A

E

N

L
|
L

T

V

V

M

G

D

M

M

P

P

K

K

F

S

I

L

T

C

A

A

G

F

T

G

G

G

L

L

D
|
D

A

A

F

V

A

T

H
|
H

C

A

V

M

E

E

A

A

F

Y

S

V

S

S

P

V

H

L

Y

A

H

S

P

E

M

F

S

S

Q

D

G

G

I

Q

A

A

L

L

E

Q

G

A

M

L

R

K

L

L

V

L

K

K

E

E

Y

Y

L

L

P

P

R

A

A

S

Y

Y

A

H

D

E

G

G

T

S

-

K

D

N

I

P

E

V

A

A

R

R

A

E

Q

R

M

V

M

H

S

S

A

A

A

T

M

I

G

A

A

G

T

I

A

A

F

F

Q

A

K

N

G

A

-

F

L

L

G

G

A

V

I

C

H
|
H

A

S

M

M

S

A

H

H

P

K

I

L

G

G

A

S

L

Q

F

F

N

H

T

I

H

P

H
|
H

G

G

T

L

T

A

N

N

A

A

V

L

C

L

M

I

L

C

A

N

V

V

L

I

A

R

F

Y

N

N

A

A

-

N

-

D

-

N

-

P

-

T

-

K

-

Q

-

T

-

A

-

F

-

S

-

Q

-

Y

-

D

-

R

P

P

E

Q

I

A

A

R

D

R

R

R

F

Y

K

A

T

E

A

I

A

A

D

D

Y

H

L

L

G

G

I

L

D

S

-

A

-

P

-

G

-

D

-

R

-

T

-

A

G

A

G

K

F

I

E

E

G

K

V

L

C

L

A

A

Y

W

V

L

Q

E

E

T

L

L

N

K

D

A

S

E

L

L

G

G

I

I

P

P

R

K

G

S

L

I

S

R

E

E

L

A

G

G

V

V

T

Q

E

E

-

A

-

D

-

F

-

L

A

A

A

N

I

V

P

D

E

K

L

L

V

S

K

E

G

D

A

A

L

F

I

D

D

D

P

Q

S

C

C

T

G

G

G

A

N

N

P

P

V

R

V

Y

L

P

D

L

Q

I

A

S

N

E

L

L

T

K

Q

Q

L

I

F

L

K

L

D

D

D487 (= D40) binding
D519 (≠ N72) binding
GSPMD 546:550 (≠ GSGLD 99:103) binding
TT 597:598 (= TT 144:145) binding
L638 (= L185) binding
D653 (= D200) binding
H657 (= H204) binding
H723 (= H268) binding
H737 (= H282) binding

7bvpA Adhe spirosome in extended conformation (see paper)
32% identity, 97% coverage: 10:379/381 of query aligns to 457:860/869 of 7bvpA

query
sites
7bvpA

P

P

T

K

A

S

I

I

K

Y

F

F

G

R

A

R

G

G

R

S

I

L

R

P

E

I

L

A

A

L

E

D

A

E

C

V

A

I

T

T

A

D

G

G

M

H

K

K

K

R

P

A

L

L

L

I

V

V

T

T

D
|
D

K

R

G
x
F

L

L

A

F

D

N

L

N

P

G

V

Y

T

A

Q

D

S

Q

T

I

L

T

D

S

I

V

L

L

E

K

A

A

G

G

L

V

G

E

R

T

A

E

M

V

F

F

C

F

E

E

V

V

D

E

P
x
A

N
x
D

P
|
P

N

T

E

L

K

S

N

I

L

V

E

R

A

K

G

G

V

A

A

E

A

L

Y

A

K

N

A

S

G

F

D

K

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

G
|
G

S
|
S

G

P

L

M

D
|
D

L

A

G

A

K

K

M

I

V

M

A

W

F

V

M

M

C

Y

G

-

Q

-

N

E

R

H

P

P

V

E

W

T

D

H

F

F

E

E

D

E

I

L

G

A

-

L

-

R

-

F

-

M

D

D

W

I

W

R

T

K

R

R

A

I

-

Y

-

K

-

F

-

P

D

K

A

M

D

G

A

V

I

K

A

A

P

K

I

M

I

I

A

A

V

V

P

T

T
|
T

A

S

G

G

T
|
T

G

G

S
|
S

E

E

V

V

G

T

R

P

A
x
F

S

A

V
|
V

I

V

T

T

D

D

S

D

V

A

T

T

H

G

Q

Q

K
|
K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

A

V

L

L

T

P

P

T

D

V

M

V

A

I

I

C

V

D

D

P

A

E

N

L

L

T

V

V

M

G

D

M
|
M

P

P

K

K

F

S

I
x
L

T

C

A

A

G

F

T

G

G

G

L

L

D
|
D

A

A

F

V

A

T

H
|
H

C

A

V

M

E

E

A

A

F

Y

S

V

S

S

P

V

H

L

Y

A

H

S

P

E

M

F

S

S

Q

D

G

G

I

Q

A

A

L

L

E

Q

G

A

M

L

R

K

L

L

V

L

K

K

E

E

Y

Y

L

L

P

P

R

A

A

S

Y

Y

A

H

D

E

G

G

T

S

-

K

D

N

I

P

E

V

A

A

R

R

A

E

Q

R

M

V

M

H

S

S

A

A

A

T

M

I

G

A

A

G

T

I

A

A

F
|
F

Q

A

K

N

G

A

-

F

L

L

G

G

A

V

I

C

H
|
H

A

S

M

M

S

A

H

H

P

K

I

L

G

G

A

S

L

Q

F

F

N

H

T

I

H

P

H
|
H

G

G

T

L

T

A

N

N

A

A

V

L

C

L

M

I

L

C

A

N

V

V

L

I

A

R

F

Y

N

N

A

A

-

N

-

D

-

N

-

P

-

T

-

K

-

Q

-

T

-

A

-

F

-

S

-

Q

-

Y

-

D

-

R

P

P

E

Q

I

A

A

R

D

R

R

R

F

Y

K

A

T

E

A

I

A

A

D

D

Y

H

L

L

G

G

I

L

D

S

-

A

-

P

-

G

-

D

-

R

-

T

-

A

G

A

G

K

F

I

E

E

G

K

V

L

C

L

A

A

Y

W

V

L

Q

E

E

T

L

L

N

K

D

A

S

E

L

L

G

G

I

I

P

P

R

K

G

S

L

I

S

R

E

E

L

A

G

G

V

V

T

Q

E

E

-

A

-

D

-

F

-

L

A

A

A

N

I

V

P

D

E

K

L

L

V

S

K

E

G

D

A

A

L

F

I

D

D

D

P

Q

S

C

C

T

G

G

G

A

N

N

P

P

V

R

V

Y

L

P

D

L

Q

I

A

S

N

E

L

L

T

K

Q

Q

L

I

F

L

K

L

D

D

binding nicotinamide-adenine-dinucleotide: D487 (= D40), F489 (≠ G42), A518 (≠ P71), D519 (≠ N72), P520 (= P73), G546 (= G99), S547 (= S100), D550 (= D103), T597 (= T144), T598 (= T145), T601 (= T148), S603 (= S150), F608 (≠ A155), V610 (= V157), K619 (= K166), M642 (= M189), L646 (≠ I193), F714 (= F260), H737 (= H282)
binding zinc ion: D653 (= D200), H657 (= H204), H723 (= H268), H737 (= H282)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 110, 111, 112, 113, 114, 139, 193, 194, 195, 197, 198, 201, 212, 213, 214, 246, 335, 337, 367, 417, 418, 419

6tqmA Escherichia coli adhe structure in its compact conformation (see paper)
32% identity, 97% coverage: 10:379/381 of query aligns to 457:860/869 of 6tqmA

query
sites
6tqmA

P

P

T

K

A

S

I

I

K

Y

F

F

G

R

A

R

G

G

R

S

I

L

R

P

E

I

L

A

A

L

E

D

A

E

C

V

A

I

T

T

A

D

G

G

M

H

K

K

K

R

P

A

L

L

L

I

V

V

T

T

D
|
D

K

R

G

F

L
|
L

A

F

D

N

L

N

P

G

V

Y

T

A

Q

D

S

Q

T

I

L

T

D

S

I

V

L

L

E

K

A

A

G

G

L

V

G

E

R

T

A

E

M

V

F

F

C

F

E

E

V

V

D

E

P

A

N

D

P

P

N

T

E

L

K

S

N

I

L

V

E

R

A

K

G

G

V

A

A

E

A

L

Y

A

K

N

A

S

G

F

D

K

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

G
|
G

S
|
S

G

P

L

M

D
|
D

L

A

G

A

K

K

M

I

V

M

A

W

F

V

M

M

C

Y

G

-

Q

-

N

E

R

H

P

P

V

E

W

T

D

H

F

F

E

E

D

E

I

L

G

A

-

L

-

R

-

F

-

M

D

D

W

I

W

R

T

K

R

R

A

I

-

Y

-

K

-

F

-

P

D

K

A

M

D

G

A

V

I

K

A

A

P

K

I

M

I

I

A

A

V

V

P

T

T
|
T

T

T

A

S

G

G

T

T

G

G

S
|
S

E

E

V

V

G

T

R

P

A
x
F

S
x
A

V
|
V

I

V

T

T

D

D

S

D

V

A

T

T

H

G

Q

Q

K

K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

A

V

L

L

T

P

P

T

D

V

M

V

A

I

I

C

V

D

D

P

A

E

N

L

L

T

V

V

M

G

D

M

M

P

P

K

K

F

S

I
x
L

T

C

A

A

G

F

T

G

G

G

L

L

D
|
D

A

A

F

V

A

T

H
|
H

C

A

V

M

E

E

A

A

F

Y

S

V

S

S

P

V

H

L

Y

A

H

S

P

E

M

F

S

S

Q

D

G

G

I

Q

A

A

L

L

E

Q

G

A

M

L

R

K

L

L

V

L

K

K

E

E

Y

Y

L

L

P

P

R

A

A

S

Y

Y

A

H

D

E

G

G

T

S

-

K

D

N

I

P

E

V

A

A

R

R

A

E

Q

R

M

V

M

H

S

S

A

A

A

T

M

I

G

A

A

G

T

I

A

A

F

F

Q

A

K

N

G

A

-

F

L

L

G

G

A

V

I

C

H
|
H

A

S

M

M

S

A

H
|
H

P

K

I

L

G

G

A

S

L

Q

F

F

N

H

T

I

H

P

H
|
H

G

G

T

L

T

A

N

N

A

A

V

L

C

L

M

I

L

C

A

N

V

V

L

I

A

R

F

Y

N

N

A

A

-

N

-

D

-

N

-

P

-

T

-

K

-

Q

-

T

-

A

-

F

-

S

-

Q

-

Y

-

D

-

R

P

P

E

Q

I

A

A

R

D

R

R

R

F

Y

K

A

T

E

A

I

A

A

D

D

Y

H

L

L

G

G

I

L

D

S

-

A

-

P

-

G

-

D

-

R

-

T

-

A

G

A

G

K

F

I

E

E

G

K

V

L

C

L

A

A

Y

W

V

L

Q

E

E

T

L

L

N

K

D

A

S

E

L

L

G

G

I

I

P

P

R

K

G

S

L

I

S

R

E

E

L

A

G

G

V

V

T

Q

E

E

-

A

-

D

-

F

-

L

A

A

A

N

I

V

P

D

E

K

L

L

V

S

K

E

G

D

A

A

L

F

I

D

D

D

P

Q

S

C

C

T

G

G

G

A

N

N

P

P

V

R

V

Y

L

P

D

L

Q

I

A

S

N

E

L

L

T

K

Q

Q

L

I

F

L

K

L

D

D

binding fe (iii) ion: D653 (= D200), H657 (= H204), H723 (= H268), H737 (= H282)
binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: D487 (= D40), L490 (= L43), G545 (= G98), G546 (= G99), S547 (= S100), D550 (= D103), T597 (= T144), S603 (= S150), F608 (≠ A155), A609 (≠ S156), V610 (= V157), L646 (≠ I193), H727 (= H272), H737 (= H282)

3zdrA Structure of the alcohol dehydrogenase (adh) domain of a bifunctional adhe dehydrogenase from geobacillus thermoglucosidasius ncimb 11955 (see paper)
29% identity, 98% coverage: 7:381/381 of query aligns to 5:400/403 of 3zdrA

query
sites
3zdrA

W

F

S

K

Y

V

P

P

T

P

A

K

I

I

K

Y

F

F

G

E

A

K

G

N

R

A

I

V

R

Q

E

Y

L

L

A

A

E

K

A

-

C

-

A

-

T

M

A

P

G

D

M

I

K

S

K

R

P

A

L

F

L

I

V

V

T

T

D

D

K

P

G

G

L

M

A

V

D

K

L

L

P

G

V

Y

T

V

Q

D

S

K

T

V

L

L

D

Y

I

Y

L

L

E

R

A

R

G

P

-

D

-

Y

L

V

G

H

R

S

A

E

M

I

F

F

C

S

E

E

V

V

D

E

P

P

N

D

P

P

N

S

E

I

K

E

N

T

L

V

E

M

A

K

G

G

V

V

A

D

A

M

Y

M

K

R

A

S

G

F

D

E

H

P

D

D

G

V

V

I

I

I

A

A

F

L

G

G

S

S

G

P

L

M

D

D

L

A

G

A

K

K

M

-

V

-

A

A

F

M

M

W

C

L

G

F

Q

Y

N

E

R

H

P

P

V

T

W

A

D

D

F

F

E

N

D

A

I

L

G

K

D

Q

W

K

W

F

T

L

R

D

A

I

D

R

A

K

D

R

A

V

I

Y

-

K

-

Y

-

P

-

K

-

L

-

G

-

Q

-

K

A

A

P

K

I

F

I

V

A

A

V

I

P

P

T

T

A

S

G

G

T

T

G

G

S

S

E

E

V

V

G

T

R

S

A

F

S

A

V

V

I

I

T

T

D

D

S

K

V

K

T

T

H

N

Q

I

K

K

K

Y

I

P

I

L

F

A

H

D

P

Y

K

E

V

L

L

T

P

P

T

D

V

V

V

A

I

I

C

V

D

D

P

P

E

Q

L

F

T

V

V

M

G

T

M

V

P

P

K

K

F

H

I

V

T

T

A

A

G

D

T

T

G

G

L

M

D
|
D

A

V

F

L

A

T

H
|
H

C

A

V

I

E

E

A

A

F

Y

S

V

S

S

P

N

H

M

Y

A

H

N

P

D

M

Y

S

T

Q

D

G

G

I

L

A

A

L

M

E

K

G

A

M

I

R

Q

L

L

V

V

K

F

E

E

Y

Y

L

L

P

P

R

R

A

A

Y

Y

A

Q

D

N

G

G

T

A

D

D

I

E

E

L

A

A

R

R

A

E

Q

K

M

M

M

H

S

N

A

A

A

S

A

T

M

I

G

A

A

G

T

M

A

A

F

F

Q

A

K

N

G

A

-

F

L

L

G

G

A

I

I

N

H
|
H

A

S

M

L

S

A

H

H

P

K

I

L

G

G

A

A

L

E

F

F

N

H

T

I

H

P

H
|
H

G

G

T

R

T

A

N

N

A

T

V

I

C

L

M

M

L

P

A

H

V

V

L

I

A

R

F

Y

N

N

A

A

P

A

E

K

I

P

A

K

D

K

R

Y

F

F

K

K

T

A

A

D

A

Q

D

R

Y

Y

L

A

G

E

I

I

-

A

-

R

-

M

-

L

-

G

-

L

-

P

-

A

-

R

-

T

D

E

G

E

G

G

F

V

E

E

G

S

V

L

C

V

A

Q

Y

A

V

I

Q

I

E

K

L

L

N

A

D

K

S

Q

L

L

G

D

I

M

P

P

R

L

G

S

L

I

S

E

E

A

L

C

G

G

V

V

T

S

-

K

-

Q

-

E

-

F

E

E

A

S

A

K

I

V

P

E

E

K

L

L

V

A

K

E

G

L

A

A

L

F

I

E

D

D

P

Q

S

C

C

T

G

T

G

A

N

N

P

P

V

K

V

L

L

P

D

L

Q

V

A

S

N

D

L

L

T

V

Q

H

L

I

F

Y

K

R

D

Q

A

A

M

F

binding zinc ion: D203 (= D200), H207 (= H204), H272 (= H268), H286 (= H282)

1rrmA Crystal structure of lactaldehyde reductase
31% identity, 96% coverage: 15:380/381 of query aligns to 13:381/385 of 1rrmA

query
sites
1rrmA

F

F

G

G

A

R

G

G

R

A

I

V

R

G

E

A

L

L

A

T

E

D

A

E

C

V

A

K

T

R

A

R

G

G

M

Y

K

Q

K

K

P

A

L

L

L

I

V

V

T

T

D
|
D

K

K

G
x
T

L
|
L

A

V

D

Q

L

C

P

G

V

V

T

V

Q

A

S

K

T

V

L

T

D

D

I

K

L

M

E

D

A

A

G

G

L

L

G

A

R

W

A

A

M

I

F

Y

C

D

E

G

V

V

D

V

P
|
P

N
|
N

P

P

N

T

E

I

K

T

N

V

L

V

E

K

A

E

G

G

V

L

A

G

A

V

Y

F

K

Q

A

N

G

S

D

G

H

A

D

D

G

Y

V

L

I

I

A

A

F

I

G

G

G
|
G

S
|
S

G

P

L

Q

D

D

L

T

G

C

K

K

M

A

V

I

A

G

F

I

M

I

C

-

G

-

Q

S

N

N

R

N

P

P

V

-

W

-

D

E

F

F

E

A

D

D

I

V

G

R

D

S

W

L

W

E

T

G

R

L

A

S

D

P

A

T

D

N

A

K

I

P

A

S

-

V

P

P

I

I

I

L

A

A

V

I

P

P

T
|
T

A

A

G

G

T
|
T

G

A

S

A

E

E

V

V

G

T

R

I

A

N

S

Y

V
|
V

I

I

T

T

D

D

S

E

V

E

T

K

H

R

Q

R

K
|
K

K

F

I

V

I

C

F

V

H

D

P

P

K

H

V

D

L

I

P

P

T

Q

V

V

V

A

I

F

C

I

D

D

P

A

E

D

L

M

T
x
M

V

D

G
|
G

M
|
M

P
|
P

K

P

F

A