SitesBLAST
Comparing GFF2696 FitnessBrowser__WCS417:GFF2696 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
53% identity, 100% coverage: 1:249/249 of query aligns to 1:249/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S137), H147 (≠ F147), Y150 (= Y150), L188 (= L188), L246 (≠ F246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (= N15), S16 (= S16), G17 (= G17), I18 (= I18), R38 (= R38), R39 (= R39), D60 (= D60), V61 (≠ I61), N87 (= N87), S88 (≠ A88), G89 (= G89), V110 (≠ I110), T135 (= T135), S137 (= S137), Y150 (= Y150), K154 (= K154), P180 (= P180), G181 (= G181), A182 (≠ P182), I183 (= I183), T185 (= T185), S187 (≠ G187)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
53% identity, 100% coverage: 1:249/249 of query aligns to 1:249/249 of 4bmsF
- active site: S137 (= S137), H147 (≠ F147), Y150 (= Y150), K154 (= K154), Q195 (≠ T195)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (= N15), S16 (= S16), I18 (= I18), R38 (= R38), R39 (= R39), A59 (≠ G59), D60 (= D60), V61 (≠ I61), N87 (= N87), S88 (≠ A88), G89 (= G89), V110 (≠ I110), S137 (= S137), Y150 (= Y150), K154 (= K154), G181 (= G181), I183 (= I183), T185 (= T185), I187 (≠ G187)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
41% identity, 99% coverage: 1:246/249 of query aligns to 6:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), S20 (≠ N15), K21 (≠ S16), G22 (= G17), I23 (= I18), A43 (≠ R38), S44 (≠ R39), S45 (≠ Q40), G68 (= G59), D69 (= D60), V70 (≠ I61), N96 (= N87), S97 (≠ A88), G98 (= G89), Y100 (≠ G91), I144 (≠ T135), S146 (= S137), Y159 (= Y150), K163 (= K154), P189 (= P180), G190 (= G181), M191 (≠ P182), I192 (= I183), T194 (= T185), G196 (= G194), T197 (= T195)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S137), Y159 (= Y150), M191 (≠ P182), I202 (≠ A200)
4esoB Crystal structure of a putative oxidoreductase protein from sinorhizobium meliloti 1021 in complex with NADP
39% identity, 98% coverage: 5:248/249 of query aligns to 4:246/251 of 4esoB
- active site: G16 (= G17), S136 (= S137), M146 (≠ F147), Y149 (= Y150), K153 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), T14 (≠ N15), H15 (≠ S16), M17 (≠ I18), R37 (= R38), N38 (≠ R39), N41 (≠ E42), S58 (≠ G59), D59 (= D60), I60 (= I61), N86 (= N87), A87 (= A88), G88 (= G89), T134 (= T135), S136 (= S137), Y149 (= Y150), P179 (= P180), G180 (= G181), I182 (= I183), T184 (= T185), T186 (≠ G187), K187 (≠ L188), G188 (≠ D189)
5t2uA Short chain dehydrogenase/reductase family protein (see paper)
43% identity, 98% coverage: 3:245/249 of query aligns to 3:237/241 of 5t2uA
- active site: G17 (= G17), T135 (≠ S137), T145 (≠ F147), Y148 (= Y150), K152 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), G17 (= G17), R38 (= R38), D39 (≠ R39), R42 (≠ E42), D60 (= D60), L61 (≠ I61), N83 (= N87), A84 (= A88), Y87 (≠ Q94), I133 (≠ L134), T135 (≠ S137), Y148 (= Y150), K152 (= K154), P178 (= P180), P180 (= P182), T181 (≠ I183), T183 (= T185), T185 (≠ S193), T186 (≠ G194)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
37% identity, 98% coverage: 1:245/249 of query aligns to 4:248/259 of 6ci9D
- active site: G20 (= G17), S145 (= S137), Y159 (= Y150)
- binding 1-aminopropan-2-one: F97 (≠ G91), S145 (= S137), T147 (= T139), W156 (≠ F147), Y159 (= Y150), G190 (= G181)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G13), S18 (≠ N15), G20 (= G17), I21 (= I18), G40 (= G37), R41 (= R38), N42 (≠ R39), D66 (= D60), V67 (≠ I61), N93 (= N87), G95 (= G89), T143 (= T135), S145 (= S137), Y159 (= Y150), K163 (= K154), P189 (= P180), N191 (≠ P182), I192 (= I183), T194 (= T185), G196 (= G187), L197 (= L188)
7ejiB Crystal structure of kred f147l/l153q/y190p/l199a/m205f/m206f variant and methyl methacrylate complex
38% identity, 98% coverage: 3:246/249 of query aligns to 3:248/251 of 7ejiB
- binding methyl 2-methylprop-2-enoate: S142 (= S137), I143 (≠ T138), Y155 (= Y150), F205 (≠ I201)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ N15), L16 (≠ S16), G17 (= G17), I18 (= I18), R38 (= R38), H39 (≠ R39), D62 (= D60), A63 (≠ I61), N89 (= N87), A90 (= A88), V112 (≠ I110), M140 (≠ T135), S142 (= S137), Y155 (= Y150), K159 (= K154), P187 (= P180), P189 (= P182), I190 (= I183), T192 (= T185), P193 (= P186), L194 (≠ G187)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
38% identity, 98% coverage: 3:246/249 of query aligns to 5:250/253 of 7ejhA
- binding 2-oxidanylisoindole-1,3-dione: S144 (= S137), I145 (≠ T138), E146 (≠ T139), Y157 (= Y150), V197 (≠ L188), F207 (≠ I201)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), T17 (≠ N15), I20 (= I18), R40 (= R38), H41 (≠ R39), D64 (= D60), A65 (≠ I61), N91 (= N87), A92 (= A88), V114 (≠ I110), M142 (≠ T135), S144 (= S137), Y157 (= Y150), K161 (= K154), P189 (= P180), G190 (= G181), P191 (= P182), I192 (= I183), T194 (= T185), P195 (= P186), L196 (≠ G187)
1zk4A Structure of r-specific alcohol dehydrogenase (wildtype) from lactobacillus brevis in complex with acetophenone and NADP (see paper)
37% identity, 98% coverage: 2:246/249 of query aligns to 2:248/251 of 1zk4A
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1-phenylethanone: A93 (≠ G91), Y155 (= Y150), Y189 (≠ P182)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ N15), L16 (≠ S16), I18 (= I18), T36 (≠ V36), G37 (= G37), R38 (= R38), H61 (≠ G59), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ L90), Y155 (= Y150), G188 (= G181), I190 (= I183), T192 (= T185), L194 (≠ G187)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 99% coverage: 1:246/249 of query aligns to 2:242/244 of 4nbuB
- active site: G18 (= G17), N111 (= N111), S139 (= S137), Q149 (≠ F147), Y152 (= Y150), K156 (= K154)
- binding acetoacetyl-coenzyme a: D93 (≠ F93), K98 (≠ S98), S139 (= S137), N146 (≠ T144), V147 (≠ P145), Q149 (≠ F147), Y152 (= Y150), F184 (≠ P182), M189 (≠ G187), K200 (≠ D204)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ S16), G18 (= G17), I19 (= I18), D38 (≠ E42), F39 (≠ L43), V59 (≠ G59), D60 (= D60), V61 (≠ I61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (≠ L90), T137 (= T135), S139 (= S137), Y152 (= Y150), K156 (= K154), P182 (= P180), F184 (≠ P182), T185 (≠ I183), T187 (= T185), M189 (≠ G187)
Q6WVP7 NADP-dependent (R)-specific alcohol dehydrogenase; (R)-specific ADH; Ketoreductase; KRED; EC 1.1.1.- from Lentilactobacillus kefiri (Lactobacillus kefiri) (see paper)
38% identity, 98% coverage: 3:246/249 of query aligns to 4:249/252 of Q6WVP7
Sites not aligning to the query:
6y0sAAA R-specific alcohol dehydrogenase (see paper)
36% identity, 98% coverage: 2:246/249 of query aligns to 2:248/251 of 6y0sAAA
1zk1A Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
36% identity, 98% coverage: 2:246/249 of query aligns to 2:248/251 of 1zk1A
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1-phenylethanone: A93 (≠ G91), N95 (≠ F93), Y155 (= Y150), Y189 (≠ P182)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ S16), I18 (= I18), D37 (≠ G37), H61 (≠ G59), D62 (= D60), S63 (≠ I61), N89 (= N87), A90 (= A88), I92 (≠ L90), M140 (≠ T135), Y155 (= Y150), G188 (= G181), I190 (= I183), L194 (≠ G187)
1zjzA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
36% identity, 98% coverage: 2:246/249 of query aligns to 2:248/251 of 1zjzA
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ S16), I18 (= I18), D37 (≠ G37), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ L90), Y155 (= Y150), G188 (= G181), I190 (= I183), L194 (≠ G187)
- binding (1r)-1-phenylethanol: A93 (≠ G91), N95 (≠ F93), L152 (≠ F147), Y155 (= Y150)
1zjyA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and nadh (see paper)
36% identity, 98% coverage: 2:246/249 of query aligns to 2:248/251 of 1zjyA
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G13), L16 (≠ S16), G17 (= G17), I18 (= I18), D37 (≠ G37), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ L90), Y155 (= Y150), G188 (= G181), I190 (= I183), L194 (≠ G187)
- binding (1r)-1-phenylethanol: A93 (≠ G91), N95 (≠ F93), L152 (≠ F147), Y155 (= Y150), Y189 (≠ P182)
3r3sA Structure of the ygha oxidoreductase from salmonella enterica
38% identity, 98% coverage: 3:245/249 of query aligns to 44:288/292 of 3r3sA
- active site: G58 (= G17), S184 (= S137), L194 (≠ F147), Y197 (= Y150), K201 (= K154), Q242 (≠ E199)
- binding magnesium ion: D56 (≠ N15), S57 (= S16), E82 (≠ R39)
- binding nicotinamide-adenine-dinucleotide: D56 (≠ N15), S57 (= S16), G58 (= G17), I59 (= I18), L79 (≠ V36), E82 (≠ R39), D106 (= D60), L107 (≠ I61), V133 (≠ N87), A134 (= A88), G135 (= G89), S184 (= S137), Y197 (= Y150), K201 (= K154), P227 (= P180), G228 (= G181), I230 (= I183), T232 (= T185), L234 (= L190), Q235 (≠ A191)
P0AG84 Uncharacterized oxidoreductase YghA; EC 1.-.-.- from Escherichia coli (strain K12) (see paper)
39% identity, 98% coverage: 3:245/249 of query aligns to 46:290/294 of P0AG84
Sites not aligning to the query:
- 39 modified: N6-acetyllysine
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
35% identity, 98% coverage: 3:245/249 of query aligns to 2:240/251 of 6xewA
- active site: G16 (= G17), S138 (= S137), Y151 (= Y150)
- binding r,3-hydroxybutan-2-one: S138 (= S137), S140 (≠ T139), Y151 (= Y150)
- binding s,3-hydroxybutan-2-one: S138 (= S137), Y151 (= Y150), S182 (≠ G181)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (≠ G37), W37 (≠ R38), W37 (≠ R38), A38 (≠ R39), I59 (≠ G59), D60 (= D60), V61 (≠ I61), N87 (= N87), A88 (= A88), G89 (= G89), V110 (≠ I110), T136 (= T135), S138 (= S137), Y151 (= Y150), K155 (= K154), S182 (≠ G181), L183 (≠ P182), V184 (≠ I183), T186 (= T185), N187 (≠ P186), M188 (≠ G187), T189 (≠ L188)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
35% identity, 98% coverage: 3:245/249 of query aligns to 2:240/251 of 6vspA
- active site: G16 (= G17), S138 (= S137), Y151 (= Y150)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (≠ G37), W37 (≠ R38), W37 (≠ R38), A38 (≠ R39), I59 (≠ G59), D60 (= D60), V61 (≠ I61), N87 (= N87), A88 (= A88), G89 (= G89), V90 (≠ L90), V110 (≠ I110), T136 (= T135), S138 (= S137), Y151 (= Y150), K155 (= K154), P181 (= P180), S182 (≠ G181), L183 (≠ P182), V184 (≠ I183), T186 (= T185), N187 (≠ P186), M188 (≠ G187), T189 (≠ L188)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
35% identity, 98% coverage: 3:245/249 of query aligns to 2:240/251 of H9XP47
- N15 (≠ S16) binding
- M17 (≠ I18) binding
- D36 (≠ G37) binding
- D60 (= D60) binding
- V61 (≠ I61) binding
- N87 (= N87) binding
- S138 (= S137) binding ; binding
- V139 (≠ T138) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ T139) binding
- Y151 (= Y150) binding ; binding ; binding
- K155 (= K154) binding
- V184 (≠ I183) binding
- T186 (= T185) binding
- RDK 197:199 (≠ IDD 202:204) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
Query Sequence
>GFF2696 FitnessBrowser__WCS417:GFF2696
MSRLNGKIAVVTGGNSGIGLATAIRFATEGAQVVIVGRRQDELDKALALIGHEAIAIQGD
ISKLDDLARIFTQIKADKGRVDVLFANAGLGDFQPIGSITEESFDRTFGINVKGTLFTVQ
NALPLMHAGSSVILTGSTTGTMGTPAFSVYSATKAALRNFARSWALDLKGSGIRVNVLSP
GPISTPGLDLALSGTGQKEAIIDDMTAQVPLGRIGKPEEVAAAALFLASDESSFMTGSEM
FVDGGFAQV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory