SitesBLAST
Comparing GFF2711 FitnessBrowser__Marino:GFF2711 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
56% identity, 96% coverage: 5:487/503 of query aligns to 3:481/489 of 4zz7A
- active site: N149 (= N152), K172 (= K175), L246 (= L249), C280 (= C283), E382 (= E388), A462 (= A468)
- binding nicotinamide-adenine-dinucleotide: T146 (= T149), P147 (= P150), F148 (= F151), N149 (= N152), K172 (= K175), E175 (≠ P178), K205 (= K208), V208 (= V211), F222 (= F225), V223 (= V226), G224 (= G227), S225 (= S228), I228 (= I231), L246 (= L249), G247 (= G250), C280 (= C283), E382 (= E388), F384 (= F390)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
57% identity, 96% coverage: 6:487/503 of query aligns to 3:455/468 of 5tjrD
- active site: N144 (= N152), K167 (= K175), L241 (= L249), C270 (= C283), E356 (= E388), A436 (= A468)
- binding adenosine-5'-diphosphate: I140 (≠ V148), T141 (= T149), F143 (= F151), K167 (= K175), E170 (≠ P178), K200 (= K208), F217 (= F225), S220 (= S228), I223 (= I231)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
54% identity, 96% coverage: 8:491/503 of query aligns to 9:489/491 of 4iymC
- active site: N153 (= N152), K176 (= K175), F250 (≠ L249), C284 (= C283), E386 (= E388), Q466 (≠ A468)
- binding nicotinamide-adenine-dinucleotide: I149 (≠ V148), T150 (= T149), P151 (= P150), F152 (= F151), N153 (= N152), F154 (= F153), K176 (= K175), K209 (= K208), V212 (= V211), F226 (= F225), V227 (= V226), G228 (= G227), S229 (= S228), I232 (= I231), G251 (= G250), C284 (= C283), E386 (= E388), F388 (= F390)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
46% identity, 96% coverage: 6:487/503 of query aligns to 7:481/487 of P42412
- C36 (≠ G35) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R106) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T149) binding
- F152 (= F151) binding
- C160 (≠ M159) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K175) binding
- E179 (≠ P178) binding
- R180 (≠ L179) binding
- S229 (= S228) binding
- T251 (≠ G250) binding
- R283 (= R282) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I286) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ V350) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E388) binding
- C413 (≠ S419) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
46% identity, 96% coverage: 6:487/503 of query aligns to 5:479/484 of 1t90A
- active site: N151 (= N152), K174 (= K175), L248 (= L249), C282 (= C283), E380 (= E388), A460 (= A468)
- binding nicotinamide-adenine-dinucleotide: I147 (≠ V148), A148 (≠ T149), P149 (= P150), F150 (= F151), N151 (= N152), W159 (= W160), K174 (= K175), E177 (≠ P178), R178 (≠ L179), H207 (≠ K208), V225 (= V226), G226 (= G227), S227 (= S228), V230 (≠ I231), L248 (= L249), T249 (≠ G250), C282 (= C283), E380 (= E388), F382 (= F390)
2d4eC Crystal structure of the hpcc from thermus thermophilus hb8
34% identity, 95% coverage: 8:483/503 of query aligns to 29:501/515 of 2d4eC
- active site: N173 (= N152), K196 (= K175), E271 (≠ L249), C305 (= C283), E409 (= E388), E486 (≠ A468)
- binding nicotinamide-adenine-dinucleotide: I169 (≠ V148), T170 (= T149), P171 (= P150), W172 (≠ F151), K196 (= K175), A198 (≠ S177), G229 (≠ D207), G233 (≠ V211), A234 (≠ N212), T248 (≠ V226), G249 (= G227), E250 (≠ S228), T253 (≠ I231), E271 (≠ L249), L272 (≠ G250), C305 (= C283), E409 (= E388), F411 (= F390), F475 (= F455)
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
32% identity, 95% coverage: 7:483/503 of query aligns to 15:486/497 of 8skfA
- binding calcium ion: T33 (≠ V25), I34 (≠ F26), D100 (≠ E92), V187 (≠ L179)
- binding nicotinamide-adenine-dinucleotide: I156 (≠ V148), G157 (≠ T149), A158 (≠ P150), W159 (≠ F151), K183 (= K175), E186 (≠ P178), G216 (≠ D207), G220 (≠ V211), T235 (≠ V226), G236 (= G227), G237 (≠ S228), S240 (≠ I231), K243 (≠ N233), E259 (≠ L249), C293 (= C283), F396 (= F390)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8vqwC
- binding coenzyme a: I147 (≠ V148), W150 (≠ F151), K174 (= K175), S176 (= S177), E177 (≠ P178), G207 (≠ D207), G211 (≠ V211), F225 (= F225), G227 (= G227), G228 (≠ S228), S231 (≠ I231), H331 (≠ A330), F387 (= F390)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8uznA
- binding adenosine monophosphate: I147 (≠ V148), G148 (≠ T149), K174 (= K175), S176 (= S177), E177 (≠ P178), G207 (≠ D207), G211 (≠ V211), F225 (= F225), G228 (≠ S228), S231 (≠ I231), K234 (≠ N233)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (≠ T149), W150 (≠ F151), K174 (= K175), S176 (= S177), E177 (≠ P178), G207 (≠ D207), G211 (≠ V211), F225 (= F225), T226 (≠ V226), G227 (= G227), G228 (≠ S228), S231 (≠ I231), E250 (≠ L249), G252 (= G251), C284 (= C283), E385 (= E388), F387 (= F390)
8uzkA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADP+ bound)
32% identity, 95% coverage: 7:483/503 of query aligns to 6:477/488 of 8uzkA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I147 (≠ V148), G148 (≠ T149), W150 (≠ F151), N151 (= N152), K174 (= K175), S176 (= S177), E177 (≠ P178), G207 (≠ D207), G211 (≠ V211), F225 (= F225), G227 (= G227), G228 (≠ S228), S231 (≠ I231), E250 (≠ L249), F387 (= F390)
1bpwA Betaine aldehyde dehydrogenase from cod liver (see paper)
29% identity, 93% coverage: 20:489/503 of query aligns to 35:498/503 of 1bpwA
- active site: N166 (= N152), K189 (= K175), E263 (≠ L249), C297 (= C283), E400 (= E388), E477 (≠ T466)
- binding nicotinamide-adenine-dinucleotide: I162 (≠ V148), L163 (≠ T149), W165 (≠ F151), N166 (= N152), K189 (= K175), G221 (≠ D207), G225 (≠ V211), T240 (≠ V226), G241 (= G227), S242 (= S228), T245 (≠ I231), E263 (≠ L249), L264 (≠ G250), C297 (= C283), E400 (= E388), F402 (= F390), F466 (= F455)
P56533 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
29% identity, 93% coverage: 20:489/503 of query aligns to 35:498/503 of P56533
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
30% identity, 96% coverage: 9:489/503 of query aligns to 10:485/490 of Q9HTJ1
- GAWN 150:153 (≠ TPFN 149:152) binding
- K162 (≠ M161) active site, Charge relay system
- KPSE 176:179 (≠ KPSP 175:178) binding
- G209 (≠ K208) binding
- GTST 230:233 (≠ STPI 228:231) binding
- E252 (≠ L249) active site, Proton acceptor
- C286 (= C283) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E388) binding
- E464 (≠ T466) active site, Charge relay system
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
30% identity, 96% coverage: 9:489/503 of query aligns to 9:484/489 of 4cazA
- active site: N152 (= N152), K175 (= K175), E251 (≠ L249), C285 (= C283), E386 (= E388), E463 (≠ T466)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ V148), G149 (≠ T149), W151 (≠ F151), N152 (= N152), K175 (= K175), E178 (≠ P178), G208 (≠ K208), G212 (≠ N212), F226 (= F225), T227 (≠ V226), G228 (= G227), G229 (≠ S228), T232 (≠ I231), V236 (≠ L234), E251 (≠ L249), L252 (≠ G250), C285 (= C283), E386 (= E388), F388 (= F390)
Query Sequence
>GFF2711 FitnessBrowser__Marino:GFF2711
MTNATIQHYINGEISLGTSSQSQDVFNPATGQVTGRVALAGREDVDAAVAAADAAFPAWA
DTPPIRRARVMFKFLELLNTHKDDLARAITAEHGKVFTDAQGEVARGIDIVEFACGIPQL
LKGDYTEQVSTGIDNWTTRQPLGVVAGVTPFNFPAMVPMWMFPVAIAAGNTFVLKPSPLD
PSASLMIADLLKQAGLPDGVFNVVQGDKDAVNALIEHPDVQALSFVGSTPIANLLYEKGA
KHGKRIQALGGAKNHMVVMPDADLDKAVDALIGAAYGSAGERCMAISVAVLVGDVADKIV
PRLAERARSLKVKNGEQLDAEMGPIVTAAAHQRITGYIDKGVAEGAELVVDGRGFDASNT
GDGCADGFWMGGSLFDHVTPDMTIYREEIFGPVLACVRVPDIATAIRLINDHEFGNGVSC
FTESGSVAREFGRRIQVGMVGINVPIPVPMAWHGFGGWKRSMFGDTHAYGEEGVKFYTRQ
KSIMQRWSDSIDAGAEFVMPTAK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory