SitesBLAST
Comparing GFF2712 FitnessBrowser__Marino:GFF2712 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
35% identity, 98% coverage: 9:552/556 of query aligns to 1:450/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G19), G13 (= G21), S14 (≠ T22), A15 (= A23), E35 (= E43), A36 (= A44), W47 (= W70), P65 (= P88), G67 (= G90), V180 (= V237), A214 (= A276), G215 (= G277), A218 (≠ G280), T270 (= T331), Y391 (≠ F491), A424 (= A526), I435 (≠ T537), N436 (= N538)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
33% identity, 99% coverage: 1:549/556 of query aligns to 72:633/654 of A0A248QE08
- TA 93:94 (= TA 22:23) binding
- E114 (= E43) binding
- L162 (≠ T92) binding
- S166 (≠ C96) binding
- NATL 170:173 (≠ NGML 100:103) binding
- V298 (= V237) binding
- C432 (≠ S362) binding
- R451 (≠ H382) binding
- Y466 (vs. gap) binding
- Q486 (≠ S404) binding
- G622 (≠ N538) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 97% coverage: 11:552/556 of query aligns to 6:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H382), N499 (≠ F491)
- binding flavin-adenine dinucleotide: G14 (= G19), G16 (= G21), T17 (= T22), A18 (= A23), L37 (≠ I42), E38 (= E43), A39 (= A44), F58 (≠ I63), W64 (= W70), A82 (≠ P88), G89 (= G95), S90 (≠ C96), N94 (= N100), A95 (≠ G101), T96 (≠ M102), L97 (= L103), M191 (≠ N206), V222 (= V237), C264 (≠ S275), A265 (= A276), G266 (= G277), H269 (≠ G280), N499 (≠ F491), A534 (= A526), Q544 (≠ N536), T545 (= T537), G546 (≠ N538)
- binding heptadecane: V377 (≠ Q384), G379 (vs. gap), M380 (≠ L386), G386 (= G392), T389 (vs. gap), Y390 (vs. gap), F393 (vs. gap), T408 (= T402), Q410 (≠ S404)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
34% identity, 99% coverage: 3:552/556 of query aligns to 4:526/532 of 4mjwA
- active site: I333 (≠ L364), P377 (= P410), N378 (≠ T411), V464 (≠ I490), H466 (= H492), V509 (≠ G535), N510 (= N536)
- binding flavin-adenine dinucleotide: G20 (= G19), G22 (= G21), S23 (≠ T22), E44 (= E43), A45 (= A44), W71 (= W70), R89 (= R89), A90 (≠ G90), G95 (= G95), C96 (= C96), H99 (≠ I99), N100 (= N100), S101 (≠ G101), I103 (≠ L103), R231 (≠ H236), A232 (≠ V237), T269 (≠ A276), G270 (= G277), D273 (≠ G280), Y465 (≠ F491), H466 (= H492), A500 (= A526), N510 (= N536), P511 (≠ T537), N512 (= N538), V515 (≠ T541)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
34% identity, 99% coverage: 3:552/556 of query aligns to 4:526/527 of 2jbvA
- active site: I333 (≠ L364), P377 (= P410), N378 (≠ T411), V464 (≠ I490), H466 (= H492), V509 (≠ G535), N510 (= N536)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G21), S23 (≠ T22), E44 (= E43), A45 (= A44), W71 (= W70), A90 (≠ G90), G95 (= G95), C96 (= C96), H99 (≠ I99), N100 (= N100), S101 (≠ G101), I103 (≠ L103), R231 (≠ H236), A232 (≠ V237), T269 (≠ A276), G270 (= G277), D273 (≠ G280), V464 (≠ I490), Y465 (≠ F491), H466 (= H492), D499 (= D525), A500 (= A526), N510 (= N536), P511 (≠ T537), N512 (= N538), V515 (≠ T541)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
34% identity, 99% coverage: 3:552/556 of query aligns to 4:526/530 of 3ljpA
- active site: I333 (≠ L364), P377 (= P410), N378 (≠ T411), A464 (≠ I490), H466 (= H492), V509 (≠ G535), N510 (= N536)
- binding dihydroflavine-adenine dinucleotide: G22 (= G21), S23 (≠ T22), E44 (= E43), A45 (= A44), W71 (= W70), R89 (= R89), A90 (≠ G90), G95 (= G95), C96 (= C96), H99 (≠ I99), N100 (= N100), S101 (≠ G101), I103 (≠ L103), A232 (≠ V237), T269 (≠ A276), D273 (≠ G280), Y465 (≠ F491), H466 (= H492), D499 (= D525), A500 (= A526), N510 (= N536), P511 (≠ T537), N512 (= N538), V515 (≠ T541)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
33% identity, 99% coverage: 1:552/556 of query aligns to 12:569/578 of 5nccA
- active site: R347 (= R344), L420 (≠ V405), I421 (≠ C406), S507 (≠ I490), A509 (≠ H492), G552 (= G535), Q553 (≠ N536)
- binding flavin-adenine dinucleotide: G30 (= G19), G32 (= G21), T33 (= T22), A34 (= A23), L53 (≠ I42), E54 (= E43), A55 (= A44), F74 (≠ I63), W80 (= W70), A98 (≠ P88), G100 (= G90), G105 (= G95), S106 (≠ C96), N110 (= N100), A111 (≠ G101), T112 (≠ M102), L113 (= L103), V238 (= V237), A278 (= A276), H282 (≠ G280), L286 (= L284), N508 (≠ F491), Q553 (≠ N536), T554 (= T537), G555 (≠ N538), V558 (≠ T541)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
31% identity, 98% coverage: 12:554/556 of query aligns to 1:563/565 of 5oc1A
- active site: V339 (≠ I345), N413 (≠ A403), A414 (≠ S404), I499 (= I490), H501 (= H492), A544 (≠ G535), H545 (≠ N536)
- binding 4-methoxybenzoic acid: Y91 (≠ G101), I356 (vs. gap), I390 (≠ D375), F396 (≠ Y381), T412 (= T402), I499 (= I490), H501 (= H492), H545 (≠ N536)
- binding flavin-adenine dinucleotide: G8 (= G19), G10 (= G21), N11 (≠ T22), A12 (= A23), E32 (= E43), A33 (= A44), W60 (= W70), P78 (= P88), G80 (= G90), G85 (= G95), S86 (≠ C96), H90 (≠ N100), Y91 (≠ G101), V93 (≠ L103), V230 (= V237), S270 (= S275), A271 (= A276), G272 (= G277), F500 (= F491), H545 (≠ N536), T546 (= T537), Q547 (≠ N538), I550 (≠ T541)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 98% coverage: 12:554/556 of query aligns to 1:563/565 of 3fimB
- active site: V339 (≠ I345), N413 (≠ A403), A414 (≠ S404), I499 (= I490), H501 (= H492), A544 (≠ G535), H545 (≠ N536)
- binding flavin-adenine dinucleotide: G8 (= G19), N11 (≠ T22), A12 (= A23), E32 (= E43), A33 (= A44), W60 (= W70), P78 (= P88), G80 (= G90), G85 (= G95), S86 (≠ C96), H90 (≠ N100), Y91 (≠ G101), V93 (≠ L103), V230 (= V237), S270 (= S275), A271 (= A276), F500 (= F491), H501 (= H492), H545 (≠ N536), T546 (= T537), Q547 (≠ N538), I550 (≠ T541)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
35% identity, 97% coverage: 13:553/556 of query aligns to 6:528/531 of E4QP00
- V101 (≠ I99) mutation to H: Abolishes activity.
- M103 (≠ G101) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ E380) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ H382) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I490) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F491) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H492) mutation to A: Abolishes activity.
- N511 (= N536) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
35% identity, 97% coverage: 13:553/556 of query aligns to 2:524/525 of 4udqA
- active site: L331 (≠ I345), F364 (≠ Y381), W365 (≠ H382), V461 (≠ I490), H463 (= H492), A506 (≠ G535), N507 (= N536)
- binding flavin-adenine dinucleotide: G8 (= G19), G10 (= G21), T11 (= T22), A12 (= A23), E32 (= E43), A33 (= A44), W64 (vs. gap), G88 (= G90), G93 (= G95), G94 (≠ C96), N98 (= N100), M99 (≠ G101), V101 (≠ L103), V229 (= V237), T261 (≠ S275), A262 (= A276), W462 (≠ F491), H463 (= H492), A497 (= A526), N507 (= N536), T508 (= T537), N509 (= N538), T512 (= T541)
8bxlB Patulin synthase from penicillium expansum
33% identity, 97% coverage: 10:549/556 of query aligns to 11:583/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G19), G22 (= G21), T23 (= T22), A24 (= A23), E44 (= E43), A45 (= A44), W80 (= W70), G100 (= G90), G105 (= G95), S106 (≠ C96), R109 (≠ I99), N110 (= N100), Y111 (≠ G101), A113 (≠ L103), L253 (≠ H236), A254 (≠ V237), A288 (= A276), Q292 (≠ G280), F525 (= F491), D559 (= D525), A560 (= A526), H570 (≠ N536), P571 (≠ T537), Q572 (≠ N538), L575 (≠ T541)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 97% coverage: 14:550/556 of query aligns to 3:502/509 of 3t37A
- active site: F360 (≠ V405), G361 (≠ C406), H444 (≠ I490), H446 (= H492), G487 (= G535), P488 (≠ N536)
- binding flavin-adenine dinucleotide: G8 (= G19), G10 (= G21), S11 (≠ T22), A12 (= A23), E32 (= E43), A33 (= A44), W58 (= W70), R77 (= R89), G78 (= G90), R79 (≠ K91), G83 (= G95), S84 (≠ C96), H88 (≠ N100), A89 (≠ G101), G91 (≠ L103), R217 (≠ H236), V218 (= V237), A251 (= A276), E255 (≠ G280), H445 (≠ F491), A478 (= A526), P488 (≠ N536), I489 (≠ T537), H490 (≠ N538)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 97% coverage: 14:550/556 of query aligns to 3:502/508 of 4ha6A
- active site: F360 (≠ V405), G361 (≠ C406), H444 (≠ I490), H446 (= H492), G487 (= G535), P488 (≠ N536)
- binding flavin-adenine dinucleotide: G8 (= G19), G10 (= G21), S11 (≠ T22), A12 (= A23), E32 (= E43), A33 (= A44), W58 (= W70), R77 (= R89), G78 (= G90), G83 (= G95), S84 (≠ C96), L87 (≠ I99), H88 (≠ N100), A89 (≠ G101), M90 (= M102), G91 (≠ L103), V218 (= V237), A251 (= A276), G252 (= G277), E255 (≠ G280), H445 (≠ F491), A478 (= A526), P488 (≠ N536), I489 (≠ T537), H490 (≠ N538)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G101), S314 (= S338), H444 (≠ I490), H446 (= H492)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 97% coverage: 12:552/556 of query aligns to 15:572/577 of 4h7uA
- active site: A343 (vs. gap), V426 (≠ C406), Y510 (≠ I490), H512 (= H492), A555 (≠ G535), H556 (≠ N536)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G19), G24 (= G21), T25 (= T22), A26 (= A23), E46 (= E43), A47 (= A44), W74 (= W70), G99 (= G95), C100 (= C96), H103 (≠ I99), N104 (= N100), G105 (= G101), V107 (≠ L103), L242 (vs. gap), V243 (vs. gap), G282 (≠ A276), G283 (= G277), A286 (≠ G280), H512 (= H492), A546 (= A526), H556 (≠ N536), T557 (= T537), Q558 (≠ N538), V561 (≠ T541)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 97% coverage: 12:552/556 of query aligns to 40:597/602 of Q3L245
- N100 (≠ R71) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I99) modified: Tele-8alpha-FAD histidine
- N344 (= N313) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H492) active site, Proton acceptor
- H581 (≠ N536) active site
Sites not aligning to the query:
3q9tA Crystal structure analysis of formate oxidase (see paper)
31% identity, 99% coverage: 7:554/556 of query aligns to 1:571/577 of 3q9tA
- active site: A335 (≠ R344), D422 (≠ C406), A508 (≠ I490), H510 (= H492), C552 (≠ G535), R553 (≠ N536)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G21), T16 (= T22), E37 (= E43), A38 (= A44), W65 (= W70), T85 (vs. gap), R86 (= R89), G87 (= G90), G92 (= G95), S93 (≠ C96), N97 (= N100), Y98 (≠ G101), F99 (≠ M102), T100 (≠ L103), S229 (≠ V237), S265 (= S275), Q266 (≠ A276), E270 (≠ G280), F509 (= F491), D542 (= D525), A543 (= A526), R553 (≠ N536), I554 (≠ T537), Q555 (≠ N538), V558 (≠ T541)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
31% identity, 99% coverage: 7:554/556 of query aligns to 1:571/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G21), T16 (= T22), E37 (= E43), A38 (= A44), W65 (= W70), T85 (vs. gap), R86 (= R89), G87 (= G90), G92 (= G95), S93 (≠ C96), N97 (= N100), Y98 (≠ G101), T100 (≠ L103), S229 (≠ V237), S265 (= S275), Q266 (≠ A276), E270 (≠ G280), F509 (= F491), D542 (= D525), A543 (= A526), A553 (≠ N536), I554 (≠ T537), Q555 (≠ N538), V558 (≠ T541)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
29% identity, 97% coverage: 13:552/556 of query aligns to 4:562/566 of 7vzsA
- binding D-glucal: Y6 (= Y15), L22 (= L31), N25 (= N34), Y51 (= Y59), I349 (≠ P356), Q356 (= Q363), E411 (≠ S404), E444 (≠ P437), W445 (≠ E438), K448 (= K441), R499 (≠ T488), N501 (≠ I490), H546 (≠ N536)
- binding flavin-adenine dinucleotide: G10 (= G19), G12 (= G21), T13 (= T22), S14 (≠ A23), E34 (= E43), A35 (= A44), Y51 (= Y59), F55 (≠ I63), W61 (= W70), R79 (≠ P88), G81 (= G90), G86 (= G95), T87 (≠ C96), N91 (= N100), G92 (= G101), M93 (= M102), A94 (≠ L103), T232 (≠ H236), A233 (≠ V237), A273 (= A276), G274 (= G277), R277 (≠ G280), F502 (= F491), A536 (= A526), H546 (≠ N536), L547 (≠ T537), V548 (≠ N538), L551 (≠ T541)
Sites not aligning to the query:
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
29% identity, 97% coverage: 13:552/556 of query aligns to 5:563/570 of 4yntA
- active site: V342 (≠ E348), F413 (≠ V405), W414 (≠ C406), N502 (≠ I490), H504 (= H492), G546 (= G535), H547 (≠ N536)
- binding dihydroflavine-adenine dinucleotide: G13 (= G21), T14 (= T22), S15 (≠ A23), E35 (= E43), A36 (= A44), F56 (≠ I63), W62 (= W70), R80 (≠ P88), G82 (= G90), G87 (= G95), T88 (≠ C96), N92 (= N100), G93 (= G101), M94 (= M102), A95 (≠ L103), A234 (≠ V237), A274 (= A276), R278 (≠ G280), F503 (= F491), A537 (= A526), H547 (≠ N536), L548 (≠ T537), V549 (≠ N538), L552 (≠ T541)
Query Sequence
>GFF2712 FitnessBrowser__Marino:GFF2712
MSDNNNATGHQEFDYIIIGAGTAGCLLANRLSANPENRVLLIEAGGRDNYHWIHIPVGYL
YCIDNPRTDWRFRTEPDPGLNGRSLIYPRGKTLGGCSSINGMLYIRGQARDYQQWAEITG
EDAWNWDNCLPDFMRHEDHYRLDEGGDADPEHRKYHGHGGEWRVEHQRLKWKVLEDFATA
CVEAGIPRTRDFNRGDNEGVDYFEVNQRSGWRWNTSKAFLRNAEKRSNLTLWHSTHVLGL
ETENAGSGPRCVGVRVERSGGDDVIARAKREVILSAGAIGSPQLLQLSGIGPAHLLKEHG
IDVVADLPGVGENLQDHLQIRSVYKVKGVTTLNTMANSLIGKARIGLEYLLTRSGPMSMA
PSQLCLFTRSSEEYDYANIEYHVQPLSLDAFGQPLHNFPAITASVCNLNPTSRGTVRIRS
NDPKQAPAISPNYLSTPEDRKVAADSLRVTRRIAEQPAFMQYQPEEFKPGLEYQTDDELA
KLAGDIGTTIFHPVGTTRMGRADDEQAVVDPHLRVRGVAGLRVVDAGVMPTITSGNTNSP
TLMIAEKAARWILAGD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory