SitesBLAST

E67 (= E74) binding
D84 (= D90) binding
L86 (≠ I92) binding
D87 (= D93) mutation to N: Loss of IMPase activity, still complements dnaB121 helicase mutation.
R139 (= R145) mutation to C: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with A-250.
G173 (≠ E177) mutation to V: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNA polymerase (RNAP).
R183 (≠ I187) mutation to A: Some growth at 30 degrees Celsius, greater IMPase activity, partially inhibited by RNAP.
R184 (= R188) mutation to A: Grows at 30 degrees Celsius, makes more dimer, partially inhibited by RNAP, crystallizes.; mutation to I: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNAP.
V250 (= V248) mutation to A: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with C-139.

Sites not aligning to the query:

251:254 KAML→AAMA: 3-fold decreased affinity for NusA, less efficient in delaying or suppressing Rho-dependent transcription termination.

6ib8B Structure of a complex of suhb and nusa ar2 domain (see paper)
36% identity, 88% coverage: 17:250/267 of query aligns to 16:256/270 of 6ib8B

query
sites
6ib8B

A

A

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A

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A

A

binding magnesium ion: D88 (= D90), L90 (≠ I92)

2qflA Structure of suhb: inositol monophosphatase and extragenic suppressor from e. Coli (see paper)
36% identity, 88% coverage: 17:250/267 of query aligns to 12:252/262 of 2qflA

query
sites
2qflA

A

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A

active site: D44 (= D51), E67 (= E74), D84 (= D90), L86 (≠ I92), D87 (= D93), T89 (= T95), D212 (= D216)
binding ethyl acetate: D87 (= D93), G186 (= G190), S187 (= S191), A188 (≠ G192)

6tqoT Rrn anti-termination complex (see paper)
37% identity, 80% coverage: 37:250/267 of query aligns to 22:244/255 of 6tqoT

query
sites
6tqoT

E

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A

A

binding magnesium ion: E59 (= E74), D76 (= D90), L78 (≠ I92)
binding : Q126 (≠ Y140), R133 (≠ K147)

Q9M8S8 Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Myo-inositol monophosphatase; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 76% coverage: 32:233/267 of query aligns to 31:238/271 of Q9M8S8

query
sites
Q9M8S8

E

E

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F

D

D

P92 (= P91) mutation to L: In vtc4-1; low levels of ascorbate.

3lv0A Crystal structure of extragenic suppressor protein suhb from bartonella henselae, native
35% identity, 87% coverage: 17:247/267 of query aligns to 11:246/258 of 3lv0A

query
sites
3lv0A

A

A

L

M

E

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A

A

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A

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G

A

A

A

L

L

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D
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D

C

M

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A

A

A

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G

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V

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E

A

A

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G

A

G

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D

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K

-

E

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G

N

N

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D

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F

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D

K

G

N

L

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A

G

G

N

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H

L

I

active site: E66 (= E74), D83 (= D90), L85 (≠ I92), D86 (= D93), T88 (= T95), D209 (= D216)
binding magnesium ion: E66 (= E74), D83 (= D90), L85 (≠ I92)

5dw8A Crystal structure of 2'amp bound saimpase-ii
32% identity, 89% coverage: 30:267/267 of query aligns to 12:252/260 of 5dw8A

query
sites
5dw8A

Q

Q

R

E

E

A

T

G

L

I

D

R

V

I

E

K

H

Q

K

L

G

M

S

E

D

Q

Q

N

D

D

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L

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N

A

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D

D

K

K

S

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E

D

D

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F

A

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L

I

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L

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P

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N

D

H

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L

L

G

G

E
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E

E

E

S

G

G

H

S

G

A

H

D

D

L

I

K

D

A

T

R

S

-

K

-

G

C

T

V

V

W

W

V

V

I

V

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T

T

A

L

C

N

F

F

V

V

N

H

G

Q

L

Q

H

E

N

N

W

F

C

A

V

I

S

S

I

I

G

G

L

I

L

Y

I

I

D

D

G

G

E

K

P

P

H

Y

I

A

G

G

A

F

I

V

A

Y

D

D

P

V

N

M

H

A

D

D

E

V

L

L

F

Y

H

H

G

A

C

K

V

V

G

G

R

E

G

G

A

A

Y

Y

V

R

N

G

Q

S

T

Q

R

P

L

L

K

K

A

P

S

L

A

N

A

D

T

S

H

N

V

L

R

R

E

Q

G

S

L

I

T

I

G

G

A

-

G

-

T

-

F

I

H

N

P

P

R

N

G

W

K

L

E

T

H

K

F

-

I

-

P

P

F

I

L

L

E

G

K

E

L

I

L

F

A

K

E

E

G

I

G

V

M

N

F

D

I

S

R

R

N

S

-

A

-

R

-

A

-

Y

G
|
G

S

S

G
x
A

A

A

L

L

M

E

T

I

A

V

Y

S

V

V

A

A

A

T

G

G

R

N

L

L

I

E

G

A

Y

Y

Y

M

E

T

T

P

E
x
R

L

L

K

Q

S

P

W

W

D
|
D

C

F

L

A

A

G

G

G

L

L

V

V

L

I

V

L

A

Y

E

E

A

V

G

N

A

G

L

Q

R

A

N

S

D

N

F

L

R

G

D

E

D

P

G

L

L

T

L

I

R

S

G

G

-

P

N

N

P

S

Y

I

L

L

V

V

A

G

A

N

P

R

G

G

V

L

Y

H

R

Q

Q

E

L

I

S

S

-

N

E

D

L

Y

I

L

G

E

P

P

S

H

L

H

D

D

A

A

binding adenosine-2'-monophosphate: D77 (= D93), G78 (= G94), G173 (= G190), A175 (≠ G192), R194 (≠ E211)
binding calcium ion: E56 (= E74), D74 (= D90), D74 (= D90), I76 (= I92), D77 (= D93), D199 (= D216)

5j16A Crystal structure of inositol monophosphate bound saimpase-ii
34% identity, 82% coverage: 50:267/267 of query aligns to 28:248/258 of 5j16A

query
sites
5j16A

A

V

D
|
D

K

K

S

A

I

T

E

E

D

D

F

F

I

I

R

F

A

D

R

T

L

I

A

L

E

E

Q

T

F

Y

P

P

Q

N

D

H

G

Q

F

V

L

L

G

G

E
|
E

E

E

S

G

G

H

S

G

A

H

D

D

L

I

K

D

A

T

R

S

-

K

-

G

C

T

V

V

W

W

V

V

I

V

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

A

L

C

N

F

F

V

V

N

H

G

Q

L

Q

H

E

N

N

W

F

C

A

V

I

S

S

I

I

G

G

L

I

L

Y

I

I

D

D

G

G

E

K

P

P

H

Y

I

A

G

G

A

F

I

V

A

Y

D

D

P

V

N

M

H

A

D

D

E

V

L

L

F

Y

H

H

G

A

C

K

V

V

G

G

R

E

G

G

A

A

Y

Y

V

R

N

G

Q

S

T

Q

R

P

L

L

K

K

A

P

S

L

A

N

A

D

T

S

H

N

V

L

R

R

E

Q

G

S

L

I

T

I

G

G

A

-

G

-

T

-

F

I

H

N

P

P

R

N

G

W

K

L

E

T

H

K

F

-

I

-

P

P

F

I

L

L

E

G

K

E

L

I

L

F

A

K

E

E

G

I

G

V

M

N

F

D

I

S

R

R

N

S

-

A

-

R

-

A

-

Y

G
|
G

S
|
S

G
x
A

A

A

L

L

M

E

T

I

A

V

Y

S

V

V

A

A

A

T

G

G

R

N

L

L

I

E

G

A

Y

Y

Y

M

E

T

T

P

E

R

L

L

K

Q

S

P

W

W

D
|
D

C

F

L

A

A

G

G

G

L

L

V

V

L

I

V

L

A

Y

E

E

A

V

G

N

A

G

L

Q

R

A

N

S

D

N

F

L

R

G

D

E

D

P

G

L

L

T

L

I

R

S

G

G

-

P

N

N

P

S

Y

I

L

L

V

V

A

G

A

N

P

R

G

G

V

L

Y

H

R

Q

Q

E

L

I

S

S

-

N

E

D

L

Y

I

L

G

E

P

P

S

H

L

H

D

D

A

A

active site: D29 (= D51), E52 (= E74), D70 (= D90), I72 (= I92), D73 (= D93), T75 (= T95), D195 (= D216)
binding calcium ion: E52 (= E74), D70 (= D90), D70 (= D90), I72 (= I92), D73 (= D93), D195 (= D216)
binding d-myo-inositol-1-phosphate: D73 (= D93), G74 (= G94), T75 (= T95), G169 (= G190), S170 (= S191), A171 (≠ G192)

3luzB Crystal structure of extragenic suppressor protein suhb from bartonella henselae, via combined iodide sad molecular replacement (see paper)
35% identity, 77% coverage: 42:247/267 of query aligns to 23:224/234 of 3luzB

query
sites
3luzB

D

D

Q

Y

Q

G

D

D

V

Y

V

V

S

S

I

Q

A

A

D

D

K

R

S

K

I

A

E

E

D

K

F

I

I

I

R

F

A

N

R

E

L

L

A

S

E

K

Q

A

F

R

P

P

Q

K

D
x
F

G

G

F

F

L

L

G

M

E
|
E

E

E

S

I

G

I

S

G

A

E

D

D

L

S

K

Q

A

H

R

R

C

-

V

-

W

F

V

I

I

V

D
|
D

P

P

I
x
L

D
|
D

G

G

T
|
T

A

T

C

N

F

F

V

L

N

H

G

G

L

I

H

P

N

F

W

F

C

A

V

V

S

S

I

I

G

A

L

L

L

E

I

S

D

Q

G

G

E

K

P

I

H

V

I

A

G

G

A

V

I

I

A

Y

D

N

P

P

N

I

H

N

D

D

E

E

L

L

F

F

H
x
T

G

A

C
x
E

V

R

G

G

R

S

G

G

A

A

Y

F

V

F

N

N

Q

D

T

R

R

R

L

C

K

R

A

V

S

S

A

A

A

R

T

R

H

R

V

L

R

E

E

D

G

C

L

V

T

I

G

A

A

T

G

G

T

M

F

-

H

-

P

-

R

-

G

-

K

-

E

-

H

-

F

Y

I

L

P

I

F

E

L

L

E

R

K

N

L

V

L

M

A

A

E

E

G

V

G

S

M

G

F

I

I

R

R

R

N

F

G

G

S

T

G

A

A

A

L

L

M

D

T

L

A

A

Y

Y

V

V

A

A

G

G

R

R

L

T

I

D

G

G

Y

F

Y

W

E

E

T

D

E

N

L

L

K

Q

S

I

W

W

D
|
D

C

M

L

A

A

A

G

G

L

I

V

L

L

M

V

V

A
x
R

E

E

A

A

G

G

A

G

L
x
F

R
x
V

-

T

-

D

-

K

-

E

-

G

N

N

D

D

F

I

F

F

R

R

D

K

G

N

L

I

R

A

G

G

N

N

P

E

Y

H

L

I

active site: E55 (= E74), D69 (= D90), L71 (≠ I92), D72 (= D93), T74 (= T95), D187 (= D216)
binding iodide ion: F50 (≠ D69), T111 (≠ H132), E113 (≠ C134), R196 (≠ A225), F201 (≠ L230), V202 (≠ R231)
binding magnesium ion: E55 (= E74), D69 (= D90), L71 (≠ I92)

Sites not aligning to the query:

binding iodide ion: 5

3luzA Crystal structure of extragenic suppressor protein suhb from bartonella henselae, via combined iodide sad molecular replacement (see paper)
35% identity, 76% coverage: 44:247/267 of query aligns to 24:228/238 of 3luzA

query
sites
3luzA

Q

E

D

D

V

Y

V

V

S

S

I

Q

A

A

D

D

K

R

S

K

I

A

E

E

D

K

F

I

I

I

R

F

A

N

R

E

L

L

A

S

E

K

Q

A

F

R

P

P

Q

K

D
x
F

G

G

F

F

L

L

G

M

E
|
E

E

E

S

S

G

E

S

E

-

I

-

G

A

E

D

D

L

S

K

Q

A

H

R

R

C

-

V

-

W

F

V

I

I

V

D
|
D

P

P

I
x
L

D
|
D

G

G

T
|
T

A

T

C

N

F

F

V

L

N

H

G

G

L

I

H

P

N

F

W

F

C

A

V

V

S

S

I

I

G

A

L

L

L

E

I

S

D

Q

G

G

E

K

P

I

H

V

I

A

G

G

A

V

I

I

A

Y

D

N

P

P

N

I

H

N

D

D

E

E

L

L

F

F

H

T

G

A

C

E

V

R

G

G

R

S

G

G

A

A

Y

F

V

F

N

N

Q

D

T

R

R

R

L

C

K

R

A

V

S

S

A

A

A

R

T

R

H

R

V

L

R

E

E

D

G

C

L

V

T

I

G

A

A

T

G

G

T

M

F

-

H

-

P

-

R

-

G

-

K

-

E

P

H

T

F

Y

I

L

P

I

F

E

L

L

E

R

K

N

L

V

L

M

A

A

E

E

G

V

G

S

M

G

F

I

I

R

R

R

N

F

G

G

S

T

G

A

A

A

L

L

M

D

T

L

A

A

Y

Y

V

V

A

A

G

G

R

R

L

T

I

D

G

G

Y

F

Y

W

E

E

T

D

E

N

L

L

K

Q

S

I

W

W

D
|
D

C

M

L

A

A

A

G

G

L

I

V
x
L

L

M

V

V

A
x
R

E

E

A

A

G

G

A

G

L

F

R

V

-

T

-

D

-

K

-

E

-

G

N

N

D

D

F

I

F

F

R

R

D

K

G

N

L

I

R

A

G

G

N

N

P

E

Y

H

L

I

active site: E54 (= E74), D71 (= D90), L73 (≠ I92), D74 (= D93), T76 (= T95), D191 (= D216)
binding iodide ion: F49 (≠ D69), L197 (≠ V222), R200 (≠ A225)
binding magnesium ion: E54 (= E74), D71 (= D90), L73 (≠ I92)

Sites not aligning to the query:

binding iodide ion: 3

O14732 Inositol monophosphatase 2; IMP 2; IMPase 2; Inositol-1(or 4)-monophosphatase 2; Myo-inositol monophosphatase A2; EC 3.1.3.25 from Homo sapiens (Human) (see 2 papers)
33% identity, 84% coverage: 10:233/267 of query aligns to 20:248/288 of O14732

query
sites
O14732

Y

F

A

Q

C

A

A

A

K

V

S

Q

L

L

A

A

L

L

E

R

A

A

A

G

Q

Q

L

I

G

-

M

-

D

-

Y

-

R

-

Q

I

R

R

E

K

T

A

L

L

D

T

V

E

E

E

H

K

K

R

G

V

S

S

D

T

Q

K

Q

T

-

S

-

A

D

D

V

L

V

V

S

T

I

E

A

T

D

D

K

H

S

L

I

V

E

E

D

D

F

L

I

I

R

I

A

S

R

E

L

L

A

R

E

E

Q

R

F

F

P

P

Q

S

D

H

G

R

F

F

L

I

G

A

E
|
E

E

E

S

A

G

A

S

A

A

S

D

G

L

-

K

-

A

A

R

K

C

C

V

V

-

L

-

T

-

H

-

S

-

P

-

T

W

W

V

I

I

I

D

D

P

P

I

I

D
|
D

G

G

T

T

A

C

C

N

F

F

V

V

N

H

G

R

L

F

H

P

N

T

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

A

I

V

D

R

G

Q

E

E

P

L

H

E

I

F

G

G

A

V

I

I

A

Y

D

H

P

C

N

T

H

E

D

E

E

R

L

L

F

Y

H

T

G

G

C

R

V

R

G

G

R

R

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

R

L

L

K

R

A

V

S

S

A

G

A

E

T

T

H

D

V

L

R

S

E

K

G

A

-

L

-

V

L

L

T

T

G

E

A

I

G

G

T

P

F

K

H

R

-

D

P

P

R

A

G

T

K

L

E

K

H

L

F

F

I

L

P

S

F

N

L

M

E

E

K

R

L

L

A

H

E

A

G

K

G

A

M

H

F

G

I

V

R

R

N

V

-

I

G

G

S

S

G

S

A

T

L

L

M

A

T

L

A

C

Y

H

V

L

A

A

A

S

G

G

R

A

L

A

I

D

G

A

Y

Y

E
x
Q

T

F

E

G

L

L

K

H

S

C

W

W

D

D

C

L

L

A

A

A

G

A

L

T

V

V

L

I

V

I

A

R

E

E

A

A

G

G

A

G

L

I

R

V

N

I

D

D

E81 (= E74) binding
D104 (= D93) mutation to N: Loss of activity.
Q224 (≠ E209) binding

2cziA Crystal structure of human myo-inositol monophosphatase 2 (impa2) with calcium and phosphate ions (see paper)
34% identity, 84% coverage: 10:233/267 of query aligns to 6:225/259 of 2cziA

query
sites
2cziA

Y

F

A

Q

C

A

A

A

K

V

S

Q

L

L

A

A

L

L

E

R

A

A

A

G

Q

Q

L

I

G

-

M

-

D

-

Y

-

R

-

Q

I

R

R

E

K

T

A

L

L

D

T

V

E

E

E

H

T

K

K

G

T

S

S

D

-

Q

A

D

D

V

L

V

V

S

T

I

E

A

T

D

D

K

H

S

L

I

V

E

E

D

D

F

L

I

I

R

I

A

S

R

E

L

L

A

R

E

E

Q

R

F

F

P

P

Q

S

D

H

G

R

F

F

L

I

G

A

E
|
E

E

E

S

A

G

K

S

C

A

V

D

-

L

L

K

T

A

H

R

S

C

P

V

T

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

A

C

C

N

F

F

V

V

N

H

G

R

L

F

H

P

N

T

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

A

I

V

D

R

G

Q

E

E

P

L

H

E

I

F

G

G

A

V

I

I

A

Y

D

H

P

C

N

T

H

E

D

E

E

R

L

L

F

Y

H

T

G

G

C

R

V

R

G

G

R

R

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

R

L

L

K

R

A

V

S

S

A

G

A

E

T

T

H

D

V

L

R

S

E

K

G

A

-

L

-

V

L

L

T

T

G

E

A

I

G

G

T

P

F

K

H

R

-

D

P

P

R

A

G

T

K

L

E

K

H

L

F

F

I

L

P

S

F

N

L

M

E

E

K

R

L

L

A

H

E

A

G

K

G

A

M

H

F

G

I

V

R

R

N

V

-

I

G

G

S

S

G

S

A

T

L

L

M

A

T

L

A

C

Y

H

V

L

A

A

A

S

G

G

R

A

L

A

I

D

G

A

Y

Y

E

Q

T

F

E

G

L

L

K

H

S

C

W

W

D
|
D

C

L

L

A

A

A

G

A

L

T

V

V

L

I

V

I

A

R

E

E

A

A

G

G

A

G

L

I

R

V

N

I

D

D

active site: E63 (= E74), D78 (= D90), I80 (= I92), D81 (= D93), T83 (= T95), D208 (= D216)
binding calcium ion: E63 (= E74), D78 (= D90), D78 (= D90), I80 (= I92), D81 (= D93), D81 (= D93), D208 (= D216)
binding phosphate ion: E63 (= E74), I80 (= I92), D81 (= D93), G82 (= G94), T83 (= T95)

2p3nA Thermotoga maritima impase tm1415 (see paper)
32% identity, 75% coverage: 34:234/267 of query aligns to 25:219/256 of 2p3nA

query
sites
2p3nA

L

V

D

D

V

N

E

V

H

E

K

K

G

K

S

T

D

G

Q

F

Q

K

D

D

V

I

V

V

S

T

I

E

A

I

D

D

K

R

S

E

I

A

E

Q

D

R

F

M

I

I

R

V

A

D

R

E

L

I

A

R

E

K

Q

F

F

F

P

P

Q

D

D

E

G

N

F

I

L

M

G

A

E
|
E

E

E

S

G

G

I

S

F

A

E

D

-

L

-

K

K

A

G

R

D

C

R

V

L

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T
|
T

A

I

C

N

F

F

V

V

N

H

G

G

L

L

H

P

N

N

W

F

C

S

V

I

S

S

I

L

G

A

L

Y

L

V

I

E

D

N

G

G

E

E

P

V

H

K

I

L

G

G

A

V

I

V

A

H

D

A

P

P

N

A

H

L

D

N

E

E

L

T

F

L

H

Y

G

A

C

E

V

E

G

G

R

S

G

G

A

A

Y

F

V

F

N

N

Q

G

T

E

R

R

L

I

K

R

A

V

S

S

A

E

A

N

T

A

H

S

V

L

R

E

E

E

G

C

L

V

T

G

G

S

A

T

G

G

T

S

F

Y

H

V

P

D

-

F

R

T

G

G

K

K

E

-

H

-

F

F

I

I

P

E

F

R

L

M

E

E

K

K

L

R

L

T

A

R

E

R

G

I

G

R

M

I

F

L

I

-

R

-

N

-

G

G

S

S

G

A

A

A

L

L

M

N

T

A

A

A

Y

Y

V

V

A

G

A

A

G

G

R

R

L

V

I

D

G

F

Y

F

Y

V

E

T

T

W

E

R

L

I

K

N

S

P

W

W

D
|
D

C

I

L

A

A

A

G

G

L

L

V

I

L

I

V

V

A

K

E

E

A

A

G

G

A

G

L

M

R

V

N

T

D

D

F

F

active site: D79 (= D90), I81 (= I92), D82 (= D93), T84 (= T95), D201 (= D216)
binding magnesium ion: E65 (= E74), D79 (= D90), I81 (= I92), D82 (= D93)

O33832 Fructose-1,6-bisphosphatase/inositol-1-monophosphatase; FBPase/IMPase; Inositol-1-phosphatase; I-1-Pase; EC 3.1.3.11; EC 3.1.3.25 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
32% identity, 75% coverage: 34:234/267 of query aligns to 25:219/256 of O33832

query
sites
O33832

L

V

D

D

V

N

E

V

H

E

K

K

G

K

S

T

D

G

Q

F

Q

K

D

D

V

I

V

V

S

T

I

E

A

I

D

D

K

R

S

E

I

A

E

Q

D

R

F

M

I

I

R

V

A

D

R

E

L

I

A

R

E

K

Q

F

F

F

P

P

Q

D

D

E

G

N

F

I

L

M

G

A

E
|
E

E

E

S

G

G

I

S

F

A

E

D

-

L

-

K

K

A

G

R

D

C

R

V

L

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D

D

G

G

T

T

A

I

C

N

F

F

V

V

N

H

G

G

L

L

H

P

N

N

W

F

C

S

V

I

S

S

I

L

G

A

L

Y

L

V

I

E

D

N

G

G

E

E

P

V

H

K

I

L

G

G

A

V

I

V

A

H

D

A

P

P

N

A

H

L

D

N

E

E

L

T

F

L

H

Y

G

A

C

E

V

E

G

G

R

S

G

G

A

A

Y

F

V

F

N

N

Q

G

T

E

R

R

L

I

K

R

A

V

S

S

A

E

A

N

T

A

H

S

V

L

R

E

E

E

G

C

L

V

T

G

G

S

A

T

G

G

T

S

F

Y

H

V

P

D

-

F

R

T

G

G

K

K

E

-

H

-

F

F

I

I

P

E

F

R

L

M

E

E

K

K

L

R

L

T

A

R

E

R

G

I

G

R

M

I

F

L

I

-

R

-

N

-

G

G

S

S

G

A

A

A

L

L

M

N

T

A

A

A

Y

Y

V

V

A

G

A

A

G

G

R

R

L

V

I

D

G

F

Y

F

Y

V

E

T

T

W

E

R

L

I

K

N

S

P

W

W

D

D

C

I

L

A

A

A

G

G

L

L

V

I

L

I

V

V

A

K

E

E

A

A

G

G

A

G

L

M

R

V

N

T

D

D

F

F

E65 (= E74) binding
D79 (= D90) binding
I81 (= I92) binding

4as5A Structure of mouse inositol monophosphatase 1 (see paper)
31% identity, 76% coverage: 31:233/267 of query aligns to 26:235/274 of 4as5A

query
sites
4as5A

R

K

E

N

T

E

L

M

D

D

V

V

E

M

H

I

K

K

G

S

S

S

D

-

Q

P

Q

A

D

D

V

L

V

V

S

T

I

V

A

T

D

D

K

Q

S

K

I

V

E

E

D

K

F

M

I

L

R

M

A

S

R

S

L

I

A

K

E

E

Q

K

F

Y

P

P

Q

C

D

H

G

S

F

F

L

I

G

G

E
|
E

E

E

S

S

G

V

S

A

A

A

D

G

L

E

K

K

A

T

-

V

-

F

-

T

-

E

R

Q

C

P

V

T

W

W

V

V

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

A

T

C

N

F

F

V

V

N

H

G

R

L

F

H

P

N

F

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

L

I

V

D

N

G

K

E

E

P

M

H

E

I

F

G

G

A

I

I

V

A

Y

D

S

P

C

N

V

H

E

D

D

E

K

L

M

F

Y

H

T

G

G

C

R

V

K

G

G

R

K

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

K

L

L

K

Q

A

V

S

S

A

Q

T

E

H

D

V

I

R

T

E

K

G

S

L

L

T

L

G

V

A

T

G

E

T

L

F

G

H

S

P

S

R

R

G

K

K

P

E

E

H

T

F

L

-

R

-

I

-

V

I

L

P

S

F

N

L

M

E

E

K

K

L

L

L

C

A

S

E

I

G

P

G

I

M

H

F

G

I

I

R

R

N

S

-

V

G

G

S

T

G

A

A

A

L

V

M

N

T

M

A

C

Y

L

V

V

A

A

A

T

G

G

R

G

L

A

I

D

G

A

Y

Y

E

E

T

M

E

G

L

I

K

H

S

C

W

W

D
|
D

C

M

L

A

A

G

G

A

L

G

V

I

L

I

V

V

A

T

E

E

A

A

G

G

A

G

L

V

R

L

N

M

D

D

active site: E68 (= E74), D88 (= D90), I90 (= I92), D91 (= D93), T93 (= T95), D218 (= D216)
binding magnesium ion: E68 (= E74), D88 (= D90), D88 (= D90), I90 (= I92), D91 (= D93), D91 (= D93), D218 (= D216)
binding phosphate ion: E68 (= E74), D88 (= D90), D91 (= D93), G92 (= G94)

2bjiA High resolution structure of myo-inositol monophosphatase, the target of lithium therapy (see paper)
30% identity, 83% coverage: 13:233/267 of query aligns to 10:235/274 of 2bjiA

query
sites
2bjiA

A

A

K

V

S

T

L

L

A

A

L

G

E

Q

A

A

A

G

Q

E

L

V

G

V

M

R

D

E

Y

A

Y

L

R

K

Q

N

R

E

E

M

T

N

L

I

D

M

V

V

E

K

H

-

K

-

G

-

S

S

D

S

Q

P

Q

A

D

D

V

L

V

V

S

T

I

A

A

T

D

D

K

Q

S

K

I

V

E

E

D

K

F

M

I

L

R

I

A

T

R

S

L

I

A

K

E

E

Q

K

F

Y

P

P

Q

S

D

H

G

S

F

F

L

I

G

G

E
|
E

E

E

S

S

G

V

S

A

A

A

D

G

L

E

K

K

A

S

-

I

-

L

-

T

-

D

R

N

C

P

V

T

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

A

T

C

N

F

F

V

V

N

H

G

G

L

F

H

P

N

F

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

V

I

V

D

N

G

K

E

K

P

M

H

E

I

F

G

G

A

I

I

V

A

Y

D

S

P

C

N

L

H

E

D

D

E

K

L

M

F

Y

H

T

G

G

C

R

V

K

G

G

R

K

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

K

L

L

K

Q

A

V

S

S

A

H

A

Q

T

E

H

D

V

I

R

T

E

K

G

S

L

L

-

L

-

V

T

T

G

E

A

L

G

G

T

S

F

S

H

R

-

T

P

P

R

E

G

T

K

V

E

R

H

I

F

I

I

L

P

S

F

N

L

I

E

E

K

R

L

L

A

C

E

L

G

P

G

I

M

H

F

G

I

I

R

R

N

G

-

V

G

G

S

T

G

A

A

A

L

L

M

N

T

M

A

C

Y

L

V

V

A

A

G

G

R

A

L

A

I

D

G

A

Y

Y

E

E

T

M

E

G

L

I

K

H

S

C

W

W

D
|
D

C

V

L

A

A

G

G

A

L

G

V

I

L

I

V

V

A

T

E

E

A

A

G

G

A

G

L

V

R

L

N

L

D

D

active site: D88 (= D90), I90 (= I92), D91 (= D93), D218 (= D216)
binding magnesium ion: E68 (= E74), D88 (= D90), D88 (= D90), I90 (= I92), D91 (= D93), D218 (= D216)

P20456 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Bos taurus (Bovine) (see paper)
30% identity, 83% coverage: 13:233/267 of query aligns to 12:237/277 of P20456

query
sites
P20456

A

A

K

V

S

T

L

L

A

A

L

G

E

Q

A

A

A

G

Q

E

L

V

G

V

M

R

D

E

Y

A

Y

L

R

K

Q

N

R

E

E

M

T

N

L

I

D

M

V

V

E

K

H

-

K

-

G

-

S

S

D

S

Q

P

Q

A

D

D

V

L

V

V

S

T

I

A

A

T

D

D

K

Q

S

K

I

V

E

E

D

K

F

M

I

L

R

I

A

T

R

S

L

I

A

K

E

E

Q

K

F

Y

P

P

Q

S

D

H

G

S

F

F

L

I

G

G

E
|
E

E

E

S

S

G

V

S

A

A

A

D

G

L

E

K

K

A

S

-

I

-

L

-

T

-

D

R

N

C

P

V

T

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

A

T

C

N

F

F

V

V

N

H

G

G

L

F

H

P

N

F

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

V

I

V

D

N

G

K

E

K

P

M

H

E

I

F

G

G

A

I

I

V

A

Y

D

S

P

C

N

L

H

E

D

D

E

K

L

M

F

Y

H

T

G

G

C

R

V

K

G

G

R

K

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

K

L

L

K

Q

A

V

S

S

A

H

A

Q

T

E

H

D

V

I

R

T

E

K

G

S

L

L

-

L

-

V

T

T

G

E

A

L

G

G

T

S

F

S

H

R

-

T

P

P

R

E

G

T

K

V

E

R

H

I

F

I

I

L

P

S

F

N

L

I

E

E

K

R

L

L

A

C

E

L

G

P

G

I

M

H

F

G

I

I

R

R

N

G

-

V

G

G

S

T

G

A

A

A

L

L

M

N

T

M

A

C

Y

L

V

V

A

A

G

G

R

A

L

A

I

D

G

A

Y

Y

E

E

T

M

E

G

L

I

K

H

S

C

W

W

D
|
D

C

V

L

A

A

G

G

A

L

G

V

I

L

I

V

V

A

T

E

E

A

A

G

G

A

G

L

V

R

L

N

L

D

D

E70 (= E74) binding
D90 (= D90) binding ; binding
I92 (= I92) binding
D93 (= D93) binding
D220 (= D216) binding

O55023 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Mus musculus (Mouse) (see paper)
31% identity, 76% coverage: 31:233/267 of query aligns to 28:237/277 of O55023

query
sites
O55023

R

K

E

N

T

E

L

M

D

D

V

V

E

M

H

I

K

K

G

S

S

S

D

-

Q

P

Q

A

D

D

V

L

V

V

S

T

I

V

A

T

D

D

K

Q

S

K

I

V

E

E

D

K

F

M

I

L

R

M

A

S

R

S

L

I

A

K

E

E

Q

K

F

Y

P

P

Q

C

D

H

G

S

F

F

L

I

G

G

E
|
E

E

E

S

S

G

V

S

A

A

A

D

G

L

E

K

K

A

T

-

V

-

F

-

T

-

E

R

S

C

P

V

T

W

W

V

F

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

A

T

C

N

F

F

V

V

N

H

G

R

L

F

H

P

N

F

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

L

I

V

D

N

G

K

E

E

P

M

H

E

I

F

G

G

A

I

I

V

A

Y

D

S

P

C

N

V

H

E

D

D

E

K

L

M

F

Y

H

T

G

G

C

R

V

K

G

G

R

K

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

K

L

L

K

Q

A

V

S

S

A

Q

T

E

H

D

V

I

R

T

E

K

G

S

L

L

T

L

G

V

A

T

G

E

T

L

F

G

H

S

P

S

R

R

G

K

K

P

E

E

H

T

F

L

-

R

-

I

-

V

I

L

P

S

F

N

L

M

E

E

K

K

L

L

L

C

A

S

E

I

G

P

G

I

M

H

F

G

I

I

R

R

N

S

-

V

G

G

S

T

G

A

A

A

L

V

M

N

T

M

A

C

Y

L

V

V

A

A

A

T

G

G

R

G

L

A

I

D

G

A

Y

Y

E

E

T

M

E

G

L

I

K

H

S

C

W

W

D
|
D

C

M

L

A

A

G

G

A

L

G

V

I

L

I

V

V

A

T

E

E

A

A

G

G

A

G

L

V

R

L

N

M

D

D

E70 (= E74) binding
D90 (= D90) binding ; binding
I92 (= I92) binding
D93 (= D93) binding
D220 (= D216) binding

P9WKI9 Inositol-1-monophosphatase SuhB; I-1-Pase; IMPase; Inositol-1-phosphatase; EC 3.1.3.25 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 66% coverage: 41:217/267 of query aligns to 50:236/290 of P9WKI9

query
sites
P9WKI9

S

S

D

S

Q

P

Q

T

D

D

V

P

V

V

S

T

I

V

A

V

D

D

K

T

S

D

I

T

E

E

D

R

F

L

I

L

R

R

A

D

R

R

L

L

A

A

E

Q

Q

L

F

R

P

P

Q

G

D

D

G

P

F

I

L
|
L

G

G

E
|
E

E

E

S

G

G

G

-

G

S

P

A

A

D

D

L

V

K

T

A

A

-

T

-

P

-

S

-

D

R

R

C

V

V

T

W

W

V

V

I

L

D
|
D

P

P

I

I

D
|
D

G

G

T

T

A

V

C

N

F

F

V

V

N

Y

G

G

L

I

H

P

N

A

W

Y

C

A

V

V

S

S

I

I

G

G

L

A

L

Q

I

V

D

G

G

G

E

I

P

T

H

V

I

A

G

G

A

A

I

V

A

A

D

D

P

V

N

A

H

A

D

R

E

T

L

V

F

Y

H

S

G

A

C

A

V

T

G

G

R

L

G

G

A

A

Y

H

V

L

N

T

Q

D

T

E

R

R

-

G

-

R

-

H

-

V

L

L

K

R

A

C

S

T

A

G

A

V

T

D

H

E

V

L

R

S

E

M

G

A

L

L

T

L

G

G

A

T

G

G

-

F

T

G

F

Y

H

S

P

V

R

R

G

C

K

R

E

E

H

K

F

Q

I

A

P

E

F

L

L

L

E

A

K

H

L

V

A

P

E

L

G

V

G

R

M

D

F

V

I

R

R

R

N

I

G

G

S

S

G

A

A

A

L

L

M

D

T

L

A

C

Y

M

V

V

A

A

G

G

R

R

L

L

I

D

G

A

Y

Y

E

E

T

H

E

G

L

V

K

Q

S

V

W
|
W

D
|
D

C

C

L81 (= L72) mutation to A: No effect on activity. 10-fold more resistant to inhibition by Li(+).
E83 (= E74) mutation to D: Less than 4% of wild-type activity.
D104 (= D90) mutation to N: Less than 4% of wild-type activity.
D107 (= D93) mutation to N: Less than 4% of wild-type activity.
W234 (= W215) mutation to L: Less than 4% of wild-type activity.
D235 (= D216) mutation to N: Less than 4% of wild-type activity.

1imdA Structural studies of metal binding by inositol monophosphatase: evidence for two-metal ion catalysis (see paper)
29% identity, 72% coverage: 41:233/267 of query aligns to 33:233/266 of 1imdA

query
sites
1imdA

S

S

D

S

Q

P

Q

V

D

D

V

L

V

V

S

T

I

A

A

T

D
|
D

K

Q

S

K

I

V

E

E

D

K

F

M

I

L

R

I

A

S

R

S

L

I

A

K

E

E

Q

K

F

Y

P

P

Q

S

D

H

G

S

F

F

L

I

G

G

E
|
E

E

E

S

S

G

V

S

A

A

A

D

G

L

E

K

K

A

S

-

I

-

L

-

T

-

D

R

N

C

P

V

T

W

W

V

I

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

A

T

C

N

F

F

V

V

N

H

G

R

L

F

H

P

N

F

W

V

C

A

V

V

S

S

I

I

G

G

L

F

L

A

I

V

D

N

G

K

E

K

P

I

H

E

I

F

G

G

A

V

I

V

A

Y

D

S

P

C

N

V

H

E

D

G

E

K

L

M

F

Y

H

T

G

A

C

R

V

K

G

G

R

K

G

G

A

A

Y

F

V

C

N

N

Q

G

T

Q

R

K

L

L

K

Q

A

V

S

S

A

Q

T

E

H

D

V

I

R

T

E

K

G

S

L

L

-

L

-

V

T

T

G

E

A

L

G

G

T

S

F

S

H

R

-

T

P

P

R

E

G

T

K

V

E

R

H

M

F

V

I

L

P

S

F

N

L

M

E

E

K

K

L

L

L

F

A

C

E

I

G

P

G

V

M

H

F

G

I

I

R

R

N

S

-

V

G

G

S

T

G

A

A

A

L

V

M

N

T

M

A

C

Y

L

V

V

A

A

A

T

G

G

R

G

L

A

I

D

G

A

Y

Y

E

E

T

M

E

G

L

I

K

H

S

C

W

W

D
|
D

C

V

L

A

A

G

G

A

L

G

V

I

L

I

V

V

A

T

E

E

A

A

G

G

A

G

L

V

R

L

N

M

D

D

active site: D43 (= D51), E66 (= E74), D86 (= D90), I88 (= I92), D89 (= D93), T91 (= T95), D216 (= D216)
binding manganese (ii) ion: E66 (= E74), D86 (= D90), D86 (= D90), I88 (= I92), D89 (= D93), D89 (= D93), D216 (= D216)
binding phosphate ion: D86 (= D90), D89 (= D93), G90 (= G94), T91 (= T95)

Query Sequence

>GFF2730 FitnessBrowser__psRCH2:GFF2730
MSNLDIDARYACAKSLALEAAQLGMDYYRQRETLDVEHKGSDQQDVVSIADKSIEDFIRA
RLAEQFPQDGFLGEESGSADLKARCVWVIDPIDGTACFVNGLHNWCVSIGLLIDGEPHIG
AIADPNHDELFHGCVGRGAYVNQTRLKASAATHVREGLTGAGTFHPRGKEHFIPFLEKLL
AEGGMFIRNGSGALMTAYVAAGRLIGYYETELKSWDCLAGLVLVAEAGALRNDFFRDDGL
LRGNPYLVAAPGVYRQLSELIGPSLDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory