SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2744 FitnessBrowser__Marino:GFF2744 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

9dl6A Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1216/1218 of 9dl6A

query
sites
9dl6A

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binding (3S)-piperidine-3-carboxylic acid: E659 (= E652), F693 (= F685), R828 (= R818), C829 (= C819), S830 (= S820), G987 (= G974), A988 (= A975)
binding flavin-adenine dinucleotide: D291 (= D288), A292 (= A289), V323 (= V320), R352 (= R349), V354 (= V351), K355 (= K352), G356 (= G353), A357 (= A354), W359 (= W356), F377 (= F374), T378 (= T375), R379 (= R376), K380 (= K377), T383 (≠ S380), A406 (= A403), T407 (= T404), H408 (= H405), N409 (= N406), Q432 (= Q428), C433 (≠ R429), Y458 (= Y452), E477 (= E471), S483 (= S477), F484 (= F478)
binding (3R)-piperidine-3-carboxylic acid: E659 (= E652), F693 (= F685), I697 (= I689), R828 (= R818), C829 (= C819), S830 (= S820), G987 (= G974), A988 (= A975), F995 (= F982)
binding nicotinamide-adenine-dinucleotide: I688 (= I680), S689 (= S681), P690 (= P682), W691 (= W683), N692 (= N684), I697 (= I689), K715 (= K707), A717 (= A709), E718 (= E710), G748 (= G740), G751 (= G744), A752 (= A745), T766 (= T759), G767 (= G760), S768 (= S761), V771 (≠ T764), I775 (= I768), E795 (= E785), T796 (= T786), C829 (= C819), E925 (= E912), F927 (= F914), F995 (= F982)

6x9dA Structure of proline utilization a with trans-4-hydroxy-l-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1216/1218 of 6x9dA

query
sites
6x9dA

M

V

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A

active site: N692 (= N684), K715 (= K707), E795 (= E785), C829 (= C819), E925 (= E912), A1007 (= A994)
binding flavin-adenine dinucleotide: D291 (= D288), A292 (= A289), V323 (= V320), Q325 (= Q322), R352 (= R349), V354 (= V351), K355 (= K352), G356 (= G353), A357 (= A354), Y358 (= Y355), W359 (= W356), F377 (= F374), T378 (= T375), R379 (= R376), K380 (= K377), T383 (≠ S380), A406 (= A403), T407 (= T404), H408 (= H405), N409 (= N406), Q432 (= Q428), C433 (≠ R429), E477 (= E471), S483 (= S477), F484 (= F478)
binding 4-hydroxyproline: E659 (= E652), F693 (= F685), I697 (= I689), R828 (= R818), S830 (= S820), G987 (= G974), A988 (= A975), F995 (= F982)
binding nicotinamide-adenine-dinucleotide: I688 (= I680), S689 (= S681), P690 (= P682), W691 (= W683), N692 (= N684), I697 (= I689), K715 (= K707), A717 (= A709), E718 (= E710), G748 (= G740), G751 (= G744), A752 (= A745), T766 (= T759), G767 (= G760), S768 (= S761), V771 (≠ T764), E795 (= E785), T796 (= T786), C829 (= C819), E925 (= E912), F927 (= F914), F995 (= F982)

7mybA Structure of proline utilization a with tetrahydrothiophene-2- carboxylate bound in the proline dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1215/1217 of 7mybA

query
sites
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A

A

binding flavin-adenine dinucleotide: D290 (= D288), A291 (= A289), V322 (= V320), Q324 (= Q322), R351 (= R349), V353 (= V351), K354 (= K352), G355 (= G353), A356 (= A354), Y357 (= Y355), W358 (= W356), F376 (= F374), T377 (= T375), R378 (= R376), K379 (= K377), T382 (≠ S380), A405 (= A403), T406 (= T404), H407 (= H405), N408 (= N406), C432 (≠ R429), L433 (= L430), E476 (= E471), S482 (= S477), F483 (= F478)
binding nicotinamide-adenine-dinucleotide: I687 (= I680), S688 (= S681), P689 (= P682), W690 (= W683), N691 (= N684), I696 (= I689), K714 (= K707), E717 (= E710), G747 (= G740), G750 (= G744), T765 (= T759), G766 (= G760), S767 (= S761), V770 (≠ T764), I774 (= I768), E794 (= E785), T795 (= T786), C828 (= C819), E924 (= E912), F926 (= F914), F994 (= F982)
binding (2S)-thiolane-2-carboxylic acid: K249 (= K247), Y457 (= Y452), Y469 (= Y464), R472 (= R467), R473 (= R468)
binding (2R)-thiolane-2-carboxylic acid: K249 (= K247), D290 (= D288), Y457 (= Y452), Y469 (= Y464), R472 (= R467), R473 (= R468)

7myaA Structure of proline utilization a with the fad covalently-modified by 1,3-dithiolane (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1215/1217 of 7myaA

query
sites
7myaA

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A

A

binding nicotinamide-adenine-dinucleotide: I687 (= I680), S688 (= S681), P689 (= P682), W690 (= W683), N691 (= N684), I696 (= I689), K714 (= K707), A716 (= A709), E717 (= E710), G747 (= G740), G750 (= G744), A751 (= A745), T765 (= T759), G766 (= G760), S767 (= S761), V770 (≠ T764), E794 (= E785), T795 (= T786), C828 (= C819), E924 (= E912), F926 (= F914), F994 (= F982)
binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[5-(1,3-dithiolan-2-yl)-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: D290 (= D288), A291 (= A289), V322 (= V320), Q324 (= Q322), V353 (= V351), K354 (= K352), G355 (= G353), A356 (= A354), W358 (= W356), F376 (= F374), T377 (= T375), R378 (= R376), K379 (= K377), T382 (≠ S380), A405 (= A403), T406 (= T404), H407 (= H405), N408 (= N406), Q431 (= Q428), C432 (≠ R429), L433 (= L430), Y457 (= Y452), E476 (= E471)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[5-(1,3-dithiolan-2-yl)-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 1217

7mycA Structure of proline utilization a with the fad covalently modified by tetrahydrothiophene (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1215/1216 of 7mycA

query
sites
7mycA

M

V

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G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

binding 1,4-dihydronicotinamide adenine dinucleotide: I687 (= I680), S688 (= S681), P689 (= P682), W690 (= W683), N691 (= N684), K714 (= K707), E717 (= E710), G747 (= G740), G750 (= G744), A751 (= A745), F764 (= F758), G766 (= G760), S767 (= S761), V770 (≠ T764), T795 (= T786), G796 (= G787), C828 (= C819), E924 (= E912), F926 (= F914)
binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-{7,8-dimethyl-2,4-dioxo-5-[(2R)-tetrahydrothiophen-2-yl]-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl}-2,3,4-trihydroxypentyl dihydrogen diphosphate (non-preferred name): K249 (= K247), D290 (= D288), A291 (= A289), V322 (= V320), Q324 (= Q322), R351 (= R349), V353 (= V351), K354 (= K352), G355 (= G353), A356 (= A354), Y357 (= Y355), W358 (= W356), F376 (= F374), T377 (= T375), R378 (= R376), K379 (= K377), T382 (≠ S380), A405 (= A403), T406 (= T404), H407 (= H405), N408 (= N406), Q431 (= Q428), C432 (≠ R429), L433 (= L430), Y457 (= Y452), S482 (= S477), F483 (= F478)

6x99A Structure of proline utilization a with d-proline bound in the l- glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1214/1216 of 6x99A

query
sites
6x99A

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

R

D

P

E

E

H

T

K

E

V

C

I

L

H

P

E

P

M

L

I

V

A

E

G

A

A

A

Q

T

L

Q

S

S

Q

K

A

E

E

I

R

R

D

D

A

A

I

A

S

A

A

S

R

T

A

A

A

R

E

K

L

L

V

I

R

E

S

A

V

L

R

R

K

-

N

-

A

G

K

K

S

G

T

S

I

G

M

V

E

E

K

G

F

L

L

V

A

Q

E

E

Y

Y

G

S

L

L

T

S

T

S

K

Q

E

E

G

G

V

V

A

A

L

L

M

M

C

C

L

L

A

A

E

E

A

A

L

L

L

L

R

R

V

I

P

P

D

D

N

T

T

A

T

T

I

R

H

D

E

A

L

L

I

I

E

R

D

D

K

K

I

I

T

A

S

D

G

G

A

N

W

W

G

K

T

S

H

H

V

L

G

S

K

R

A

-

S

-

S

S

G

L

L

F

I

V

N

N

T

A

A

A

T

T

V

W

A

G

L

L

L

V

M

V

T

T

S

G

N

K

L

L

L

T

K

S

D

T

S

V

E

N

R

D

N

R

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S

V

L

G

A

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A

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L

L

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K

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L

L

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K

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C

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G

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G

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Y

D

D

M

M

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L

G

G

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A

A

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T

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D

A

D

A

D

D

A

A

K

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Y

Y

Y

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Y

Y

S

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N

S

A

A

I

I

D

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S

A

I

I

A

G

K

K

A

A

S

S

K

A

G

G

-

R

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K

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N

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P

G

G

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K

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A

N

A

Y

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M

I

G

G

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N

N

I

I

D
|
D

A
|
A

E

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A

D

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L

L

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L

L

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D

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L

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L

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G

W

W

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N

G

G

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M

G

G

V

F

V
|
V

V

V

Q

Q

A

A

Y

Y

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G

K

K

R

R

S

C

S

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F

F

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L

L

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K

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Y

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G

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K

R

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I

M

M

V

V

R
|
R

L

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

W
|
W

D

D

A

A

E

E

I

I

K

K

R

R

A

A

Q

Q

V

L

M

D

G

G

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D

F

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P

P

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V

F
|
F

T
|
T

R
|
R

K
|
K

A

I

C

H

S
x
T

D

D

V

V

S

S

F

Y

L

I

S

A

C

C

A

A

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K

L

L

L

L

N

A

M

A

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T

N

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R

V

I

V

Y

F

P

P

Q

Q

F

F

A
|
A

T
|
T

H
|
H

N
|
N

A

A

H

Q

S

T

V

L

S

A

A

A

I

I

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-

G

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K

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K

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K

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Y

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|
Q

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x
C

L

L

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|
Y

A

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L

L

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Y

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L

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L

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|
E

N

N

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G

A

A

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S

S
|
S

F
|
F

V

V

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|
W

K
x
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G

A

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L

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A

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A

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N

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I

A

A

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I

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R

E
|
E

A

A

V

I

D

D

F

F

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L

Q

R

Y

Y

Y

Y

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A

N

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P

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L

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G

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P

M

I

C

V

C

C

I

I

S

S

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

A

I

I

F

F

T

T

G

G

Q

Q

I

I

L

A

A

A

N

A

L

L

A

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A

A

G

G

N

N

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V

V

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A

K
|
K

P

P

A

A

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E

Q

E

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T

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P

L

L

L

I

A

A

I

A

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x
R

L

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M

L

H

R

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G

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K

A

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M

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P

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-

R

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-

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P

P

L

L

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|
E

T

T

G

G

G

G

L

Q

N

N

A

A

M

M

I

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D

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D

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S

A

A

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G

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R
|
R

C
|
C

S
|
S

A

A

L

L

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R

M

V

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L

Y

C

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L

Q

Q

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F

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L

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K

I

L

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S

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D

L

L

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Q

E

R

E
|
E

I

V

F

F

G

G

P

P

V

V

L

L

H

H

V

V

A

I

T

R

F

Y

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K

D

N

D

I

L

D

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L

V

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D

D

D

D

I

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N

N

A

A

K

T

G

G

Y

Y

G

G

L

L

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T

F

F

G

G

I

L

H

H

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D

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K

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G

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N

T

L

Y

Y

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I

N

N

R

R

N

N

Q

I

I

I

G
|
G

A
|
A

I

V

V

V

G

G

S

V

Q

Q

P

P

F
|
F

G

G

G

G

E

R

G

G

L

L

S

S

G

G

T

T

G

G

P

P

K

K

A
|
A

G

G

G

G

P

P

Q

L

Y

Y

V

L

R

G

R

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F

L

L

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A

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V

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A

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D

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S

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V

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H

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T

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D

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P

-

V

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L

-

L

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D

N

F

A

A

R

K

K

W

V

L

D

D

A

G

K

K

Q

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A

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A

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A

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M

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D

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A

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N

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V

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A

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A

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D

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L

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A

-

L

L

P

P

G

G

P

P

T

V

G

G

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E

T

R

N

N

R

L

L

Y

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N

L

H

H

A

A

R

R

G

G

V

R

V

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L

L

C

-

L

L

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V

P

P

D

A

K

T

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E

T

S

A

G

L

L

-

Y

E

H

Q

Q

A

L

G

A

T

A

A

A

L

L

S

A

Q

T

G

G

N

N

K

S

V

V

V

A

V

I

I

D

A

A

P

A

G

S

T

G

Q

L

D

-

V

-

V

-

D

-

Q

Q

A

A

N

S

K

L

A

K

G

N

L

L

P

P

I

Q

V

T

G

V

A

G

Q

L

G

R

L

V

L

S

E

W

P

S

E

K

A

D

L

W

A

A

T

A

I

D

D

G

G

P

F

F

E

A

A

G

V

A

V

L

S

V

C

E

G

G

D

D

Q

A

P

E

L

R

L

I

K

R

A

A

Y

V

R

N

E

K

A

A

L

I

A

A

K

A

R

L

D

P

G

G

A

P

L

L

L

L

P

L

L

V

I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

-

R

-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

active site: N690 (= N684), K713 (= K707), E793 (= E785), C827 (= C819), E923 (= E912), A1005 (= A994)
binding d-proline: W557 (= W552), T558 (≠ K553), E657 (= E652), F691 (= F685), R727 (≠ E721), R826 (= R818), S828 (= S820), G985 (= G974), A986 (= A975), F993 (= F982)
binding flavin-adenine dinucleotide: D289 (= D288), A290 (= A289), V321 (= V320), R350 (= R349), V352 (= V351), K353 (= K352), G354 (= G353), A355 (= A354), Y356 (= Y355), W357 (= W356), F375 (= F374), T376 (= T375), R377 (= R376), K378 (= K377), T381 (≠ S380), A404 (= A403), T405 (= T404), H406 (= H405), N407 (= N406), Q430 (= Q428), C431 (≠ R429), Y456 (= Y452), E475 (= E471), S481 (= S477), F482 (= F478)

9e0cA Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1214/1216 of 9e0cA

query
sites
9e0cA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

R

D

P

E

E

H

T

K

E

V

C

I

L

H

P

E

P

M

L

I

V

A

E

G

A

A

A

Q

T

L

Q

S

S

Q

K

A

E

E

I

R

R

D

D

A

A

I

A

S

A

A

S

R

T

A

A

A

R

E

K

L

L

V

I

R

E

S

A

V

L

R

R

K

-

N

-

A

G

K

K

S

H

T

S

I

G

M

V

E

E

K

G

F

L

L

V

A

Q

E

E

Y

Y

G

S

L

L

T

S

T

S

K

Q

E

E

G

G

V

V

A

A

L

L

M

M

C

C

L

L

A

A

E

E

A

A

L

L

L

L

R

R

V

I

P

P

D

D

N

T

T

A

T

T

I

R

H

D

E

A

L

L

I

I

E

R

D

D

K

K

I

I

T

A

S

D

G

G

A

N

W

W

G

K

T

S

H

H

V

L

G

S

K

R

A

-

S

-

S

S

G

L

L

F

I

V

N

N

T

A

A

A

T

T

V

W

A

G

L

L

L

V

M

V

T

T

S

G

N

K

L

L

L

T

K

S

D

T

S

V

E

N

R

D

N

R

T

S

V

L

G

A

E

A

T

A

L

L

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T

K

R

L

L

L

I

K

S

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C

G

G

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G

A

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Q

M

A

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K

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G

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Q

F

F

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V

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G

G

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D

T

I

I

D

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A

A

Q

L

D

K

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A

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K

K

E

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Y

L

M

E

A

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K

K

G

G

Y

F

T

S

Y

Y

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S

Y

Y

D

D

M

M

L

L

G

G

E

E

A

A

A

A

R

T

T

T

D

A

D

A

D

D

A

A

K

E

R

R

Y

Y

Y

Y

D

R

S

D

Y

Y

S

E

N

S

A

A

I

I

D

H

S

A

I

I

A

G

K

K

A

A

S

S

K

A

G

G

-

R

D

G

V

I

R

Y

K

E

N

G

P

P

G

G

I

I

S

S

V

I

K
|
K

L

L

S

S

A

A

L

L

L

H

A

P

R

R

Y

Y

E

S

Y

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G

A

N

Q

K

A

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V

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M

N

G

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L

L

L

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A

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R

K

E

A

L

L

V

A

K

L

K

L

A

A

A

K

A

N

A

Y

N

D

M

I

G

G

F

L

N

N

I

I

D
|
D

A
|
A

E

E

E

E

Q

A

D

D

R

R

L

L

D

E

L

L

S

S

L

L

D

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L

I

L

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L

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C

A

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D

D

P

G

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L

L

A

S

G

G

W

W

D

N

G

G

F

M

G

G

V

F

V
|
V

V

V

Q

Q

A

A

Y

Y

G

G

K

K

R

R

S

C

S

P

F

F

V

V

L

L

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D

W

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Y

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A

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K

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K

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F

I

M

M

V

V

R
|
R

L

L

V
|
V

K
|
K

G
|
G

A
|
A

Y
|
Y

W
|
W

D

D

A

A

E

E

I

I

K

K

R

R

A

A

Q

Q

V

L

M

D

G

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F
|
F

T
|
T

R
|
R

K
|
K

A

I

C

H

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x
T

D

D

V

V

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S

F

Y

L

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C

C

A

A

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K

K

L

L

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N

A

M

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N

D

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V

I

V

Y

F

P

P

Q

Q

F

F

A
|
A

T
|
T

H
|
H

N
|
N

A

A

H

Q

S

T

V

L

S

A

A

A

I

I

L

Y

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M

M

A

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-

G

K

K

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Y

Y

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|
Q

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x
C

L

L

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M

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C

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I

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|
Y

A

A

P

P

V

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G

G

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H

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K

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L

L

L

L

A

A

Y

Y

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L

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R

R

R

R

L

L

L

L

E
|
E

N

N

G

G

A

A

N

N

S

S

S
|
S

F
|
F

V

V

N

H

Q

R

I

I

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D

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K

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D

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N

N

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K

A

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L

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D

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M

I

C

V

C

C

I
|
I

S
|
S

P
|
P

W
|
W

N
|
N

F

F

P

P

L

L

A

A

I
|
I

F

F

T

T

G

G

Q

Q

I

I

L

A

A

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N

A

L

L

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A

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G

N

N

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V

V

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A

K
|
K

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|
A

E
|
E

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L

L

L

I

A

A

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A

A

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L

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Q

L

L

V

L

P

P

G

G

T

D

G
|
G

A

-

T

R

V

V

G
|
G

A
|
A

A

A

L

L

T

V

S

A

D

A

S

A

R

E

V

T

S

A

G

G

V

V

C

M

F
|
F

T
|
T

G
|
G

S
|
S

T

T

A

E

T
x
V

A

A

Q

R

R

L

I
|
I

N

Q

K

A

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Q

M

L

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A

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L

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-

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P

P

L

L

V

I

A

A

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|
E

T
|
T

G

G

G

G

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N

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A

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M

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I

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V

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G

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Q

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R

C
|
C

S

S

A

A

L

L

R

R

M

V

L

L

Y

C

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L

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Q

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L

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L

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K

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T

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G

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G

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F

F

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P

P

P

A

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L

I

S

E

V

L

S

E

G

K

I

L

E

S

E

D

L

L

E

Q

E

R

E
|
E

I

V

F
|
F

G

G

P

P

V

V

L

L

H

H

V

V

A

I

T

R

F

Y

E

R

A

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K

D

N

D

I

L

D

D

K

R

V

L

V

V

D

D

D

D

I

V

N

N

A

A

K

T

G

G

Y

Y

G

G

L

L

T

T

F

F

G

G

I

L

H

H

S

T

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R

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L

D

D

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A

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S

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I

K

K

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A

G

G

N

N

T

L

Y

Y

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N

N

R

R

N

N

Q

I

I

I

G

G

A

A

I

V

V

V

G

G

S

V

Q

Q

P

P

F
|
F

G

G

G

G

E

R

G

G

L

L

S

S

G

G

T

T

G

G

P

P

K

K

A

A

G

G

G

G

P

P

Q

L

Y

Y

V

L

R

G

R

R

F

L

L

V

K

T

G

T

E

A

T

P

V

V

E

P

R

P

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Q

A

H

D

S

S

S

-

V

-

H

-

T

-

D

-

P

-

V

-

L

-

L

-

D

N

F

A

A

R

K

K

W

V

L

D

D

A

G

K

K

Q

G

L

A

Q

R

K

A

L

E

I

A

G

E

Q

A

L

A

D

R

K

N

L

A

K

G

A

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S

S

R

-

P

-

E

-

A

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R

-

M

-

D

-

A

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I

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R

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P

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I

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F

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G

-

N

-

V

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P

-

E

S

P

A

L

L

D

G

A

L

H

D

V

L

E

E

A

-

L

L

P

P

G

G

P

P

T

V

G

G

E

E

T

R

N

N

R

L

L

Y

S

T

N

L

H

H

A

A

R

R

G

G

V

R

V

I

L

L

C

-

L

L

G

V

P

P

D

A

K

T

E

E

T

S

A

G

L

L

-

Y

E

H

Q

Q

A

L

G

A

T

A

A

A

L

L

S

A

Q

T

G

G

N

N

K

S

V

V

V

A

V

I

I

D

A

A

P

A

G

S

T

G

Q

L

D

-

V

-

V

-

D

-

Q

Q

A

A

N

S

K

L

A

K

G

N

L

L

P

P

I

Q

V

T

G

V

A

G

Q

L

G

R

L

V

L

S

E

W

P

S

E

K

A

D

L

W

A

A

T

A

I

D

D

G

G

P

F

F

E

A

A

G

V

A

V

L

S

V

C

E

G

G

D

D

Q

A

P

E

L

R

L

I

K

R

A

A

Y

V

R

N

E

K

A

A

L

I

A

A

K

A

R

L

D

P

G

G

A

P

L

L

L

L

P

L

L

V

I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

-

R

-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

binding (1-benzofuran-5-yl)methanol: K248 (= K247), D289 (= D288), Y356 (= Y355), Y456 (= Y452)
binding flavin-adenine dinucleotide: D289 (= D288), A290 (= A289), V321 (= V320), R350 (= R349), V352 (= V351), K353 (= K352), G354 (= G353), A355 (= A354), Y356 (= Y355), W357 (= W356), F375 (= F374), T376 (= T375), R377 (= R376), K378 (= K377), T381 (≠ S380), A404 (= A403), T405 (= T404), H406 (= H405), N407 (= N406), Q430 (= Q428), C431 (≠ R429), E475 (= E471), S481 (= S477), F482 (= F478)
binding nicotinamide-adenine-dinucleotide: I686 (= I680), S687 (= S681), P688 (= P682), W689 (= W683), N690 (= N684), I695 (= I689), K713 (= K707), A715 (= A709), E716 (= E710), G746 (= G740), G749 (= G744), A750 (= A745), F763 (= F758), T764 (= T759), G765 (= G760), S766 (= S761), V769 (≠ T764), I773 (= I768), E793 (= E785), T794 (= T786), C827 (= C819), E923 (= E912), F925 (= F914), F993 (= F982)

9dl2A Structure of proline utilization a complexed with 2,3-dihydro-1,4- benzodioxine-5-carboxylic acid (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1214/1216 of 9dl2A

query
sites
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M

V

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A

A

binding 2,3-dihydro-1,4-benzodioxine-5-carboxylic acid: E657 (= E652), F691 (= F685), A694 (= A688), R826 (= R818), S828 (= S820), G985 (= G974), A986 (= A975), F993 (= F982)
binding flavin-adenine dinucleotide: D289 (= D288), A290 (= A289), V321 (= V320), R350 (= R349), V352 (= V351), K353 (= K352), G354 (= G353), A355 (= A354), Y356 (= Y355), W357 (= W356), F375 (= F374), T376 (= T375), R377 (= R376), K378 (= K377), T381 (≠ S380), A404 (= A403), T405 (= T404), H406 (= H405), N407 (= N406), Q430 (= Q428), C431 (≠ R429), E475 (= E471), N476 (= N472), S481 (= S477), F482 (= F478)
binding nicotinamide-adenine-dinucleotide: I686 (= I680), S687 (= S681), P688 (= P682), W689 (= W683), N690 (= N684), I695 (= I689), K713 (= K707), A715 (= A709), E716 (= E710), G746 (= G740), G749 (= G744), F763 (= F758), T764 (= T759), G765 (= G760), S766 (= S761), V769 (≠ T764), I773 (= I768), E793 (= E785), T794 (= T786), C827 (= C819), E923 (= E912), F925 (= F914), F993 (= F982)

7my9A Structure of proline utilization a with 1,3-dithiolane-2-carboxylate bound in the proline dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1214/1216 of 7my9A

query
sites
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A

A

binding flavin-adenine dinucleotide: D289 (= D288), A290 (= A289), V321 (= V320), Q323 (= Q322), R350 (= R349), V352 (= V351), K353 (= K352), G354 (= G353), A355 (= A354), Y356 (= Y355), W357 (= W356), F375 (= F374), T376 (= T375), R377 (= R376), K378 (= K377), T381 (≠ S380), A404 (= A403), T405 (= T404), H406 (= H405), N407 (= N406), C431 (≠ R429), L432 (= L430), E475 (= E471), S481 (= S477), F482 (= F478)
binding nicotinamide-adenine-dinucleotide: I686 (= I680), S687 (= S681), P688 (= P682), W689 (= W683), N690 (= N684), I695 (= I689), K713 (= K707), A715 (= A709), E716 (= E710), G746 (= G740), G749 (= G744), A750 (= A745), T764 (= T759), G765 (= G760), S766 (= S761), V769 (≠ T764), E793 (= E785), T794 (= T786), C827 (= C819), E923 (= E912), F925 (= F914), F993 (= F982)
binding 1,3-dithiolane-2-carboxylic acid: Y456 (= Y452), Y468 (= Y464), R471 (= R467), R472 (= R468)

6x9aA Structure of proline utilization a with trans-4-hydroxy-d-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1212/1214 of 6x9aA

query
sites
6x9aA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

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D

A

Y

A

Y

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A

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F

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D

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A

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N

E

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A

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G

D

E

-

I

-

A

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-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

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W

F

L

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V

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H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

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G

G

G

N

N

A

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S

L

L

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A

A

active site: N688 (= N684), K711 (= K707), E791 (= E785), C825 (= C819), E921 (= E912), A1003 (= A994)
binding flavin-adenine dinucleotide: D287 (= D288), A288 (= A289), V319 (= V320), R348 (= R349), V350 (= V351), K351 (= K352), G352 (= G353), A353 (= A354), Y354 (= Y355), W355 (= W356), F373 (= F374), T374 (= T375), R375 (= R376), K376 (= K377), T379 (≠ S380), A402 (= A403), T403 (= T404), H404 (= H405), N405 (= N406), C429 (≠ R429), E473 (= E471), S479 (= S477), F480 (= F478)
binding (4S)-4-hydroxy-D-proline: W555 (= W552), T556 (≠ K553), E655 (= E652), F689 (= F685), R725 (≠ E721), S826 (= S820), G983 (= G974), A984 (= A975), F991 (= F982)

9e5wA Proline utilization a (puta) from sinorhizobium meliloti inactivated by n-propargylglycine (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1212/1212 of 9e5wA

query
sites
9e5wA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

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D

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I

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F

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L

A

C

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D

N

T

T

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A

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N

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L

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A

A

binding nicotinamide-adenine-dinucleotide: I684 (= I680), S685 (= S681), P686 (= P682), W687 (= W683), N688 (= N684), I693 (= I689), K711 (= K707), A713 (= A709), E714 (= E710), G744 (= G740), G747 (= G744), A748 (= A745), T762 (= T759), G763 (= G760), S764 (= S761), V767 (≠ T764), I771 (= I768), E791 (= E785), T792 (= T786), C825 (= C819), E921 (= E912), F923 (= F914), F991 (= F982)
binding N-propargylglycine-modified flavin adenine dinucleotide: K246 (= K247), D287 (= D288), A288 (= A289), V319 (= V320), Q321 (= Q322), R348 (= R349), V350 (= V351), K351 (= K352), G352 (= G353), A353 (= A354), W355 (= W356), F373 (= F374), T374 (= T375), R375 (= R376), K376 (= K377), T379 (≠ S380), A402 (= A403), T403 (= T404), H404 (= H405), N405 (= N406), Q428 (= Q428), C429 (≠ R429), L430 (= L430), Y454 (= Y452), S479 (= S477)

6x9bA Structure of proline utilization a with cis-4-hydroxy-d-proline bound in the l-glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1212/1214 of 6x9bA

query
sites
6x9bA

M

V

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A

active site: N688 (= N684), K711 (= K707), E791 (= E785), C825 (= C819), E921 (= E912), A1003 (= A994)
binding flavin-adenine dinucleotide: D287 (= D288), A288 (= A289), V319 (= V320), R348 (= R349), V350 (= V351), K351 (= K352), G352 (= G353), A353 (= A354), Y354 (= Y355), W355 (= W356), F373 (= F374), T374 (= T375), R375 (= R376), K376 (= K377), T379 (≠ S380), A402 (= A403), T403 (= T404), H404 (= H405), N405 (= N406), Q428 (= Q428), C429 (≠ R429), Y454 (= Y452), E473 (= E471), S479 (= S477), F480 (= F478)
binding nicotinamide-adenine-dinucleotide: I684 (= I680), S685 (= S681), P686 (= P682), W687 (= W683), N688 (= N684), I693 (= I689), K711 (= K707), A713 (= A709), E714 (= E710), G744 (= G740), G747 (= G744), A748 (= A745), T762 (= T759), G763 (= G760), S764 (= S761), V767 (≠ T764), I771 (= I768), E791 (= E785), T792 (= T786), C825 (= C819), E921 (= E912), F923 (= F914)
binding (4R)-4-hydroxy-D-proline: E655 (= E652), F689 (= F685), S826 (= S820), G983 (= G974), A984 (= A975), F991 (= F982)

6x9cA Structure of proline utilization a with l-proline bound in the l- glutamate-gamma-semialdehyde dehydrogenase active site (see paper)
45% identity, 100% coverage: 1:1204/1209 of query aligns to 11:1209/1209 of 6x9cA

query
sites
6x9cA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

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V

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D

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N

N

A

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L

L

active site: N687 (= N684), K710 (= K707), E790 (= E785), C824 (= C819), E920 (= E912), A1002 (= A994)
binding dihydroflavine-adenine dinucleotide: D286 (= D288), A287 (= A289), V318 (= V320), Q320 (= Q322), R347 (= R349), V349 (= V351), K350 (= K352), G351 (= G353), A352 (= A354), Y353 (= Y355), W354 (= W356), F372 (= F374), T373 (= T375), R374 (= R376), K375 (= K377), T378 (≠ S380), A401 (= A403), T402 (= T404), H403 (= H405), N404 (= N406), Q427 (= Q428), C428 (≠ R429), E472 (= E471), S478 (= S477), F479 (= F478)
binding 1,4-dihydronicotinamide adenine dinucleotide: I683 (= I680), S684 (= S681), P685 (= P682), W686 (= W683), N687 (= N684), K710 (= K707), E713 (= E710), G743 (= G740), G746 (= G744), A747 (= A745), F760 (= F758), G762 (= G760), S763 (= S761), V766 (≠ T764), E920 (= E912), F922 (= F914)
binding proline: R823 (= R818), C824 (= C819), S825 (= S820), G982 (= G974), A983 (= A975), F990 (= F982)

5kf6A Structure of proline utilization a from sinorhizobium meliloti complexed with l-tetrahydrofuroic acid and NAD+ in space group p21 (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1207/1207 of 5kf6A

query
sites
5kf6A

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

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D

A

Y

A

Y

Y

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A

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E

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K

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I

L

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A

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A

A

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D

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G

G

P

F

F

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A

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A

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L

S

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C

E

G

G

D

D

Q

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P

E

L

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L

I

K

R

A

A

Y

V

R

N

E

K

A

A

L

I

A

A

K

A

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L

D

P

G

G

A

P

L

L

L

L

P

L

L

V

I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

-

R

-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

active site: N683 (= N684), K706 (= K707), E786 (= E785), C820 (= C819), E916 (= E912), A998 (= A994)
binding flavin-adenine dinucleotide: D282 (= D288), A283 (= A289), V314 (= V320), Q316 (= Q322), R343 (= R349), V345 (= V351), K346 (= K352), G347 (= G353), A348 (= A354), Y349 (= Y355), W350 (= W356), F368 (= F374), T369 (= T375), R370 (= R376), K371 (= K377), T374 (≠ S380), A397 (= A403), T398 (= T404), H399 (= H405), N400 (= N406), Q423 (= Q428), C424 (≠ R429), L425 (= L430), E468 (= E471), S474 (= S477), F475 (= F478)
binding nicotinamide-adenine-dinucleotide: I679 (= I680), S680 (= S681), P681 (= P682), W682 (= W683), N683 (= N684), I688 (= I689), K706 (= K707), A708 (= A709), E709 (= E710), G739 (= G740), G742 (= G744), A743 (= A745), F756 (= F758), T757 (= T759), G758 (= G760), S759 (= S761), V762 (≠ T764), I766 (= I768), E786 (= E785), T787 (= T786), C820 (= C819), E916 (= E912), F918 (= F914), F986 (= F982)
binding tetrahydrofuran-2-carboxylic acid: K241 (= K247), D282 (= D288), Y449 (= Y452), R464 (= R467), R465 (= R468)

9e0eA Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1212/1214 of 9e0eA

query
sites
9e0eA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

R

D

P

E

E

H

T

K

E

V

C

I

L

H

P

E

P

M

L

I

V

A

E

G

A

A

A

Q

T

L

Q

S

S

Q

K

A

E

E

I

R

R

D

D

A

A

I

A

S

A

A

S

R

T

A

A

A

R

E

K

L

L

V

I

R

E

S

A

V

L

R

R

K

-

N

-

A

G

K

K

S

S

T

G

I

V

M

-

E

E

K

G

F

L

L

V

A

Q

E

E

Y

Y

G

S

L

L

T

S

T

S

K

Q

E

E

G

G

V

V

A

A

L

L

M

M

C

C

L

L

A

A

E

E

A

A

L

L

L

L

R

R

V

I

P

P

D

D

N

T

T

A

T

T

I

R

H

D

E

A

L

L

I

I

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D

D

K

K

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I

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D

G

G

A

N

W

W

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S

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L

F

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N

N

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A

A

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W

A

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L

L

L

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M

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K

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|
K

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|
D

A
|
A

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|
V

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|
R

L

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|
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K
|
K

G
|
G

A
|
A

Y
|
Y

W
|
W

D

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|
F

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|
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|
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|
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|
A

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|
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|
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|
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|
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|
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|
S

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|
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|
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|
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|
W

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|
N

F

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|
I

F

F

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G

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Q

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|
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|
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L

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D

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|
G

A

-

T

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|
G

A
|
A

A

A

L

L

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A

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|
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|
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|
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|
S

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x
V

A

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|
I

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|
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|
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|
C

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L

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G

K

I

L

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S

E

D

L

L

E

Q

E

R

E
|
E

I

V

F
|
F

G

G

P

P

V

V

L

L

H

H

V

V

A

I

T

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F

Y

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K

D

N

D

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L

D

D

K

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L

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D

D

D

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N

N

A

A

K

T

G

G

Y

Y

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L

L

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F

F

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G

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L

H

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N

Q

I

I

I

G

G

A

A

I

V

V

V

G

G

S

V

Q

Q

P

P

F
|
F

G

G

G

G

E

R

G

G

L

L

S

S

G

G

T

T

G

G

P

P

K

K

A

A

G

G

G

G

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L

Y

Y

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L

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A

H

D

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-

V

-

H

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T

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D

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P

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V

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L

-

L

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D

N

F

A

A

R

K

K

W

V

L

D

D

A

G

K

K

Q

G

L

A

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K

A

L

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I

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N

L

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M

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D

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A

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I

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I

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F

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G

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N

-

V

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P

-

E

S

P

A

L

L

D

G

A

L

H

D

V

L

E

E

A

-

L

L

P

P

G

G

P

P

T

V

G

G

E

E

T

R

N

N

R

L

L

Y

S

T

N

L

H

H

A

A

R

R

G

G

V

R

V

I

L

L

C

-

L

L

G

V

P

P

D

A

K

T

E

E

T

S

A

G

L

L

-

Y

E

H

Q

Q

A

L

G

A

T

A

A

A

L

L

S

A

Q

T

G

G

N

N

K

S

V

V

V

A

V

I

I

D

A

A

P

A

G

S

T

G

Q

L

D

-

V

-

V

-

D

-

Q

Q

A

A

N

S

K

L

A

K

G

N

L

L

P

P

I

Q

V

T

G

V

A

G

Q

L

G

R

L

V

L

S

E

W

P

S

E

K

A

D

L

W

A

A

T

A

I

D

D

G

G

P

F

F

E

A

A

G

V

A

V

L

S

V

C

E

G

G

D

D

Q

A

P

E

L

R

L

I

K

R

A

A

Y

V

R

N

E

K

A

A

L

I

A

A

K

A

R

L

D

P

G

G

A

P

L

L

L

L

P

L

L

V

I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

-

R

-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

binding (1H-indol-5-yl)methanol: K247 (= K247), D288 (= D288), A354 (= A354), Y355 (= Y355), Y455 (= Y452)
binding flavin-adenine dinucleotide: D288 (= D288), A289 (= A289), V320 (= V320), R349 (= R349), V351 (= V351), K352 (= K352), G353 (= G353), A354 (= A354), Y355 (= Y355), W356 (= W356), F374 (= F374), T375 (= T375), R376 (= R376), K377 (= K377), T380 (≠ S380), A403 (= A403), T404 (= T404), H405 (= H405), N406 (= N406), Q429 (= Q428), C430 (≠ R429), S479 (= S477), F480 (= F478)
binding nicotinamide-adenine-dinucleotide: I684 (= I680), S685 (= S681), P686 (= P682), W687 (= W683), N688 (= N684), I693 (= I689), K711 (= K707), A713 (= A709), E714 (= E710), G744 (= G740), G747 (= G744), A748 (= A745), F761 (= F758), T762 (= T759), G763 (= G760), S764 (= S761), V767 (≠ T764), I771 (= I768), E791 (= E785), T792 (= T786), C825 (= C819), E921 (= E912), F923 (= F914), F991 (= F982)

9dl8A Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1204/1209 of query aligns to 12:1206/1206 of 9dl8A

query
sites
9dl8A

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

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K

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|
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L

S

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D

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D

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S

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L

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A

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L

W

A

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A

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D

D

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F

F

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A

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A

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C

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G

D

D

Q

A

P

E

L

R

L

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K

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A

A

Y

V

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N

E

K

A

A

L

I

A

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K

A

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D

P

G

G

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L

L

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I

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T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

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R

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N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

binding (4-methoxyphenyl)methanol: K242 (= K247), D283 (= D288), A284 (= A289), A349 (= A354), Y350 (= Y355), Y450 (= Y452), R466 (= R468)
binding flavin-adenine dinucleotide: D283 (= D288), A284 (= A289), V315 (= V320), Q317 (= Q322), R344 (= R349), V346 (= V351), K347 (= K352), G348 (= G353), A349 (= A354), Y350 (= Y355), W351 (= W356), F369 (= F374), T370 (= T375), R371 (= R376), K372 (= K377), T375 (≠ S380), A398 (= A403), T399 (= T404), H400 (= H405), N401 (= N406), C425 (≠ R429), E469 (= E471), S475 (= S477), F476 (= F478)
binding nicotinamide-adenine-dinucleotide: I680 (= I680), S681 (= S681), P682 (= P682), W683 (= W683), N684 (= N684), I689 (= I689), K707 (= K707), E710 (= E710), G740 (= G740), G743 (= G744), F757 (= F758), T758 (= T759), G759 (= G760), S760 (= S761), V763 (≠ T764), E787 (= E785), T788 (= T786), C821 (= C819), E917 (= E912), F919 (= F914), F987 (= F982)

9e0dA Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1212/1214 of 9e0dA

query
sites
9e0dA

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

R

D

P

E

E

H

T

K

E

V

C

I

L

H

P

E

P

M

L

I

V

A

E

G

A

A

A

Q

T

L

Q

S

S

Q

K

A

E

E

I

R

R

D

D

A

A

I

A

S

A

A

S

R

T

A

A

A

R

E

K

L

L

V

I

R

E

S

A

V

L

R

R

K

-

N

-

A

G

K

K

S

H

T

S

I

G

M

V

E

E

K

G

F

L

L

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A

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|
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|
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|
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|
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|
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|
I

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|
G

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|
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|
A

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|
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|
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|
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|
E

I

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|
F

G

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|
F

G

G

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L

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L

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A

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A

L

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D

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L

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A

-

L

L

P

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V

G

G

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N

N

R

L

L

Y

S

T

N

L

H

H

A

A

R

R

G

G

V

R

V

I

L

L

C

-

L

L

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V

P

P

D

A

K

T

E

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S

A

G

L

L

-

Y

E

H

Q

Q

A

L

G

A

T

A

A

A

L

L

S

A

Q

T

G

G

N

N

K

S

V

V

V

A

V

I

I

D

A

A

P

A

G

S

T

G

Q

L

D

-

V

-

V

-

D

-

Q

Q

A

A

N

S

K

L

A

K

G

N

L

L

P

P

I

Q

V

T

G

V

A

G

Q

L

G

R

L

V

L

S

E

W

P

S

E

K

A

D

L

W

A

A

T

A

I

D

D

G

G

P

F

F

E

A

A

G

V

A

V

L

S

V

C

E

G

G

D

D

Q

A

P

E

L

R

L

I

K

R

A

A

Y

V

R

N

E

K

A

A

L

I

A

A

K

A

R

L

D

P

G

G

A

P

L

L

L

L

P

L

L

V

I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

-

R

-

N

-

P

-

D

-

A

-

Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

E

R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

binding (2H-1,3-benzodioxol-5-yl)methanol: K248 (= K247), D289 (= D288), Y356 (= Y355), Y456 (= Y452)
binding flavin-adenine dinucleotide: D289 (= D288), A290 (= A289), V321 (= V320), Q323 (= Q322), R350 (= R349), V352 (= V351), K353 (= K352), G354 (= G353), A355 (= A354), Y356 (= Y355), W357 (= W356), F375 (= F374), T376 (= T375), R377 (= R376), K378 (= K377), T381 (≠ S380), A404 (= A403), T405 (= T404), H406 (= H405), N407 (= N406), C431 (≠ R429), S479 (= S477), F480 (= F478)
binding nicotinamide-adenine-dinucleotide: I684 (= I680), S685 (= S681), P686 (= P682), W687 (= W683), N688 (= N684), I693 (= I689), K711 (= K707), A713 (= A709), E714 (= E710), G744 (= G740), G747 (= G744), A748 (= A745), F761 (= F758), T762 (= T759), G763 (= G760), S764 (= S761), V767 (≠ T764), I771 (= I768), E791 (= E785), T792 (= T786), C825 (= C819), E921 (= E912), F923 (= F914), F991 (= F982)

9e0aB Bifunctional protein PutA (see paper)
45% identity, 100% coverage: 1:1206/1209 of query aligns to 12:1210/1212 of 9e0aB

query
sites
9e0aB

M

V

R

R

P

P

Q

Q

Q

S

S

T

Q

L

T

-

P

-

E

-

L

-

V

-

D

-

S

-

R

R

Q

R

A

A

I

I

R

T

D

A

Y

A

Y

Y

L

R

A

R

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P

E

E

H

T

K

E

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C

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L

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P

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M

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A

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A

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A

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A

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D

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A

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A

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F

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L

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N

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L

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H

A

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L

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C

-

L

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L

L

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Y

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H

Q

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A

L

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A

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L

S

A

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G

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N

K

S

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V

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I

D

A

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P

A

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S

T

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Q

L

D

-

V

-

V

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D

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Q

Q

A

A

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S

K

L

A

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L

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A

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S

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K

A

D

L

W

A

A

T

A

I

D

D

G

G

P

F

F

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A

A

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V

A

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L

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C

E

G

G

D

D

Q

A

P

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L

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L

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K

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A

A

Y

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R

N

E

K

A

A

L

I

A

A

K

A

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G

G

A

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L

L

L

L

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L

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I

Q

T

A

E

A

H

S

T

S

L

G

D

E

-

I

-

A

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R

-

N

-

P

-

D

-

A

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Y

-

C

-

L

Q

N

R

W

F

L

V

V

I

E

E

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R

V

H

S

L

A

C

S

V

I

D

N

T

T

T

A

A

A

A

A

G

G

G

G

N

N

A

A

S

S

L

L

I

M

A

A

binding (1,4-phenylene)dimethanol: K245 (= K247), D286 (= D288), A287 (= A289),