SitesBLAST
Comparing GFF2744 FitnessBrowser__WCS417:GFF2744 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8hw0A The structure of akr6d1
37% identity, 93% coverage: 1:312/336 of query aligns to 1:319/329 of 8hw0A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), W21 (vs. gap), Q27 (= Q26), D49 (= D48), Y54 (= Y53), R123 (= R121), S152 (= S150), Q178 (= Q176), W207 (≠ Y204), S208 (= S205), P209 (= P206), L210 (= L207), S212 (≠ R209), K218 (= K215), S227 (= S226), R228 (= R227), I285 (= I280), G287 (= G282), S289 (≠ R284), Q293 (= Q288), D296 (≠ A291), N297 (≠ Y292)
6ow0A Crystal structure of mithramycin 3-side chain keto-reductase mtmw in complex with NAD+ and peg (see paper)
38% identity, 93% coverage: 1:312/336 of query aligns to 1:321/323 of 6ow0A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), L21 (≠ M21), D49 (= D48), Y54 (= Y53), S151 (= S150), Y204 (= Y204), F205 (≠ S205), L207 (= L207), Q209 (≠ R209), G210 (= G210), T213 (≠ S213), K215 (= K215), R227 (= R227), V284 (≠ I280), G286 (= G282), Q292 (= Q288), N296 (≠ Y292)
Q13303 Voltage-gated potassium channel subunit beta-2; K(+) channel subunit beta-2; Kv-beta-2; hKvbeta2; EC 1.1.1.- from Homo sapiens (Human) (see 2 papers)
36% identity, 93% coverage: 2:312/336 of query aligns to 38:355/367 of Q13303
- T56 (= T20) binding
- W57 (vs. gap) binding
- Q63 (= Q26) binding
- D85 (= D48) binding
- Y90 (= Y53) mutation to F: No effect on its activity in promoting KCNA4 channel closure.
- S112 (≠ D72) modified: Phosphoserine
- S188 (= S150) binding
- R189 (≠ N151) binding
- Q214 (= Q176) binding
- W243 (≠ Y204) binding
- S244 (= S205) binding
- P245 (= P206) binding
- L246 (= L207) binding
- A247 (= A208) binding
- C248 (≠ R209) binding
- K254 (= K215) binding
- R264 (= R227) binding
- S325 (≠ R284) binding
- Q329 (= Q288) binding
- E332 (≠ A291) binding
- N333 (≠ Y292) binding
Sites not aligning to the query:
- 31 modified: Phosphoserine
7wf3C Composite map of human kv1.3 channel in apo state with beta subunits (see paper)
36% identity, 93% coverage: 2:312/336 of query aligns to 5:322/328 of 7wf3C
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G22 (= G19), R156 (≠ N151), W210 (≠ Y204), S211 (= S205), P212 (= P206), L213 (= L207), C215 (≠ R209), K221 (= K215), R231 (= R227), Q296 (= Q288), E299 (≠ A291), N300 (≠ Y292)
P62483 Voltage-gated potassium channel subunit beta-2; K(+) channel subunit beta-2; Kv-beta-2; EC 1.1.1.- from Rattus norvegicus (Rat) (see 11 papers)
36% identity, 93% coverage: 2:312/336 of query aligns to 38:355/367 of P62483
- T56 (= T20) binding
- W57 (vs. gap) binding
- Q63 (= Q26) binding
- D85 (= D48) binding
- Y90 (= Y53) mutation to F: Abolishes enzyme activity, but has no effect on NADPH binding.
- S112 (≠ D72) modified: Phosphoserine
- N158 (≠ H120) binding
- S188 (= S150) binding
- R189 (≠ N151) binding
- Q214 (= Q176) binding
- W243 (≠ Y204) binding
- S244 (= S205) binding
- P245 (= P206) binding
- L246 (= L207) binding
- A247 (= A208) binding
- C248 (≠ R209) binding
- K254 (= K215) binding
- Y262 (≠ E223) binding
- R264 (= R227) binding
- G323 (= G282) binding
- S325 (≠ R284) binding
- Q329 (= Q288) binding
- E332 (≠ A291) binding
- N333 (≠ Y292) binding
Sites not aligning to the query:
- 9 modified: Phosphoserine; S→A: Impairs interaction with MAPRE1 and association with microtubules.
- 20 modified: Phosphoserine; S→A: No effect on interaction with MAPRE1 and association with microtubules.
- 31 S→A: Impairs interaction with MAPRE1 and association with microtubules.
P62482 Voltage-gated potassium channel subunit beta-2; K(+) channel subunit beta-2; Kv-beta-2; Neuroimmune protein F5; EC 1.1.1.- from Mus musculus (Mouse) (see 2 papers)
36% identity, 93% coverage: 2:312/336 of query aligns to 38:355/367 of P62482
- Y90 (= Y53) mutation to F: No detectable phenotype.
- S112 (≠ D72) modified: Phosphoserine
Sites not aligning to the query:
- 20 modified: Phosphoserine
3eauA Voltage-dependent k+ channel beta subunit in complex with cortisone (see paper)
36% identity, 93% coverage: 2:312/336 of query aligns to 4:321/327 of 3eauA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G21 (= G19), W23 (vs. gap), Q29 (= Q26), D51 (= D48), Y56 (= Y53), K84 (= K78), S154 (= S150), Q180 (= Q176), W209 (≠ Y204), S210 (= S205), P211 (= P206), L212 (= L207), A213 (= A208), C214 (≠ R209), G215 (= G210), K220 (= K215), R230 (= R227), L287 (≠ I280), L288 (≠ V281), G289 (= G282), S291 (≠ R284), Q295 (= Q288), E298 (≠ A291), N299 (≠ Y292)
- binding 17,21-dihydroxypregna-1,4-diene-3,11,20-trione: W23 (vs. gap), V55 (= V52), Y56 (= Y53), W87 (≠ I81), N124 (≠ H120), R155 (≠ N151), I174 (≠ D170), I177 (≠ V173), I202 (≠ Y197)
1exbA Structure of the cytoplasmic beta subunit-t1 assembly of voltage- dependent k channels (see paper)
36% identity, 93% coverage: 2:312/336 of query aligns to 3:320/326 of 1exbA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G19), W22 (vs. gap), Q28 (= Q26), D50 (= D48), Y55 (= Y53), S153 (= S150), R154 (≠ N151), Q179 (= Q176), W208 (≠ Y204), S209 (= S205), P210 (= P206), L211 (= L207), C213 (≠ R209), G214 (= G210), S217 (= S213), K219 (= K215), S228 (= S226), R229 (= R227), L286 (≠ I280), G288 (= G282), S290 (≠ R284), Q294 (= Q288), E297 (≠ A291), N298 (≠ Y292)
P63144 Voltage-gated potassium channel subunit beta-1; K(+) channel subunit beta-1; Kv-beta-1; EC 1.1.1.- from Rattus norvegicus (Rat) (see paper)
36% identity, 89% coverage: 1:298/336 of query aligns to 71:373/401 of P63144
- K152 (= K78) mutation to M: Loss of enzyme activity.
Q14722 Voltage-gated potassium channel subunit beta-1; K(+) channel subunit beta-1; Kv-beta-1; EC 1.1.1.- from Homo sapiens (Human) (see paper)
36% identity, 89% coverage: 1:298/336 of query aligns to 89:391/419 of Q14722
- Y307 (= Y216) mutation to F: Reduces affinity for NADPH.
- R316 (= R227) mutation to E: Nearly abolishes NADPH binding.
6ow0B Crystal structure of mithramycin 3-side chain keto-reductase mtmw in complex with NAD+ and peg (see paper)
38% identity, 93% coverage: 1:312/336 of query aligns to 1:297/301 of 6ow0B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), L21 (≠ M21), Y50 (= Y53), H117 (= H120), S147 (= S150), Y200 (= Y204), F201 (≠ S205), L203 (= L207), Q205 (≠ R209), T209 (≠ S213), Q268 (= Q288), N272 (≠ Y292)
Q9P7U2 Putative aryl-alcohol dehydrogenase C977.14c; EC 1.1.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 93% coverage: 2:314/336 of query aligns to 8:344/351 of Q9P7U2
- S113 (vs. gap) modified: Phosphoserine
4aubB The complex structure of the bacterial aldo-keto reductase akr14a1 with NADP and citrate (see paper)
32% identity, 94% coverage: 1:315/336 of query aligns to 11:324/335 of 4aubB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G29 (= G19), W31 (≠ M21), D59 (= D48), Y64 (= Y53), H136 (= H120), Q191 (= Q176), F220 (≠ Y204), T221 (≠ S205), P222 (= P206), L223 (= L207), Q225 (≠ R209), G226 (= G210), K231 (= K215), R241 (= R230), R244 (≠ K233), L288 (≠ I280), G290 (= G282), S292 (≠ R284), Q296 (= Q288), E299 (≠ A291), N300 (≠ Y292)
Sites not aligning to the query:
1pz0A Structure of NADPH-dependent family 11 aldo-keto reductase akr11a(holo) (see paper)
32% identity, 92% coverage: 6:315/336 of query aligns to 5:311/311 of 1pz0A
- active site: D52 (= D48), Y57 (= Y53), N91 (≠ P83), H124 (= H120)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H124 (= H120), Q174 (= Q176), Y202 (= Y204), F203 (≠ S205), P204 (= P206), L205 (= L207), S207 (≠ R209), G208 (= G210), A211 (≠ S213), K213 (= K215)
3n6qD Crystal structure of yghz from e. Coli (see paper)
32% identity, 94% coverage: 1:315/336 of query aligns to 12:311/315 of 3n6qD
Q3L181 Perakine reductase; EC 1.1.1.317 from Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) (see paper)
32% identity, 89% coverage: 6:304/336 of query aligns to 6:301/337 of Q3L181
- D52 (= D48) mutation to A: 99% loss of activity.
- Y57 (= Y53) mutation to A: 99% loss of activity.
- K84 (= K78) mutation to A: Total loss of activity.
- H126 (= H120) mutation to A: 98% loss of activity.
P46336 Aldo-keto reductase IolS; AKR11A; Vegetative protein 147; VEG147; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
32% identity, 91% coverage: 6:311/336 of query aligns to 6:308/310 of P46336
5t79A X-ray crystal structure of a novel aldo-keto reductases for the biocatalytic conversion of 3-hydroxybutanal to 1,3-butanediol (see paper)
32% identity, 93% coverage: 1:312/336 of query aligns to 13:313/315 of 5t79A
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G31 (= G19), W33 (≠ M21), Y66 (= Y53), H138 (= H120), N169 (= N151), Q193 (= Q176), F221 (≠ Y204), S222 (= S205), L224 (= L207), G226 (≠ R209), T230 (≠ S213), R232 (≠ K215), S263 (≠ V263), L280 (≠ I280), G282 (= G282), S284 (≠ R284), Q288 (= Q288)
3erpA Structure of idp01002, a putative oxidoreductase from and essential gene of salmonella typhimurium (see paper)
32% identity, 93% coverage: 1:312/336 of query aligns to 12:308/312 of 3erpA
3v0sA Crystal structure of perakine reductase, founder member of a novel akr subfamily with unique conformational changes during NADPH binding (see paper)
31% identity, 94% coverage: 6:320/336 of query aligns to 6:285/287 of 3v0sA
- active site: D45 (= D48), Y50 (= Y53), G87 (≠ P88), H119 (= H120)
- binding 2'-monophosphoadenosine-5'-diphosphate: S198 (= S205), P199 (= P206), I200 (≠ L207), G201 (≠ A208), L204 (≠ V211), P246 (≠ V281), G247 (= G282), T248 (≠ P283), T249 (≠ R284), N253 (≠ Q288), N256 (≠ A291), N257 (≠ Y292)
Query Sequence
>GFF2744 FitnessBrowser__WCS417:GFF2744
MIYRTLGQSGLKVSALTLGTMMFGEQTNTEDSLRIIDKAWDQGINFIDTADVYTGGRSEE
IVGEAIVRNRQDWVVASKVGIGPVDGLPNRSGLSRKRIFNALEASLTRLDTDYLDIYYLH
REDHNTPLEVTVSAIGDLIRQGKIRYWGLSNYRGWRIAEVIRVAERLGVDKPVISQPLYN
IVNRQAEVEQITAAAAYGLGVVPYSPLARGVLSGKYAPDVTPEAGSRAARQDKRILETEW
RVESLRIAQQIQQYTQGRGVGIVEFAIAWVLNNSAVSSAIVGPRTEAQWDAYIGALEVKI
SAEDEAFIDSLVTPGHSSTPGFNDVGHFVSGRLPRA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory