SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2747 FitnessBrowser__WCS417:GFF2747 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

D7A5Q8 Aromatic amino acid exporter YddG from Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) (see paper)
33% identity, 36% coverage: 68:178/307 of query aligns to 62:171/287 of D7A5Q8

query
sites
D7A5Q8

R

R

Q

Q

L

P

L

W

Q

P

L

V

G

W

M

V

L

H

G

G

F

I

F

G

G

G

I

L

F

F

F

G

Y
|
Y

N
x
H

L

F

C

F

F
x
Y

F

F

Y

S

G

A

L

L

Q

K

S

L

I

A

N

P

A

P

S

A

R

E

A

A

S

G

L

L

I

V

V

A

A

Y

L

L

N
x
W

P

P

A

L

V

L

I

I

G

V

L

L

A

F

S

S

R

A

W

F

L

L

F

P

K

G

E

E

R

R

L

L

G

R

A

P

S

A

N

H

V

V

A

A

G

G

I

A

L

L

L

M

C

G

L

L

T

A

G

G

A

T

A

V

V

V

V

L

I

L

V

G

S

A

R

R

N

A

P

G

H

G

V

-

L

F

N

G

E

F

A

A

T

P

S

E

S

Y

W

V

R

P

G

G

D

Y

L

L

L

A

I

A

F

A

G

A

C

C

V

A

V

V

G

I

W
|
W

G

S

I

V

Y

Y

S

S

L

V

F

A

S

S

R

R

Y78 (= Y84) mutation to A: Shows moderate effects on the transport activities of both threonine and methionine.
H79 (≠ N85) mutation to A: Abolishes the transport activities for both threonine and methionine.
Y82 (≠ F88) mutation to A: Enhances methionine transport and slightly reduces threonine transport.
W101 (≠ N107) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.
W163 (= W170) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.

Query Sequence

>GFF2747 FitnessBrowser__WCS417:GFF2747
MHSSSDRITYMKLAAVTMIWGGTFVAGRYLSGTVDPLLAASLRFALASLALLGFLGLARI
PLARPSPRQLLQLGMLGFFGIFFYNLCFFYGLQSINASRASLIVALNPAVIGLASRWLFK
ERLGASNVAGILLCLTGAAVVIVSRNPHVLNEATSSWRGDLLIFGCVVGWGIYSLFSRGL
NQSLGPLQTVTWSILLGTLMLTLTTLIMGRFTLEAMGRLQTAQGLSLAYLGVLGSALAYI
GYYDGIRRIGATRSGVFIALNPLTAVICGALLLDEPLSLPMLIGGAVILLGIYLCNKPLA
RARAMGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory