Comparing GFF2758 PGA1_c28010 KHG/KDPG aldolase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
1euaA Schiff base intermediate in kdpg aldolase from escherichia coli (see paper)
52% identity, 86% coverage: 32:215/215 of query aligns to 28:212/213 of 1euaA
P0A955 KHG/KDPG aldolase; EC 4.1.3.16; EC 4.1.2.14 from Escherichia coli (strain K12) (see 3 papers)
52% identity, 86% coverage: 32:215/215 of query aligns to 28:212/213 of P0A955
6oviA Crystal structure of kdpg aldolase from legionella pneumophila with pyruvate captured at low ph as a covalent carbinolamine intermediate
46% identity, 92% coverage: 19:215/215 of query aligns to 12:209/210 of 6oviA
2c0aB Mechanism of the class i kdpg aldolase (see paper)
51% identity, 86% coverage: 32:215/215 of query aligns to 29:213/214 of 2c0aB
Sites not aligning to the query:
1wauA Structure of kdpg aldolase e45n mutant (see paper)
51% identity, 86% coverage: 32:215/215 of query aligns to 28:212/213 of 1wauA
3vcrA Crystal structure of a putative kdpg (2-keto-3-deoxy-6- phosphogluconate) aldolase from oleispira antarctica (see paper)
49% identity, 91% coverage: 20:215/215 of query aligns to 13:212/216 of 3vcrA
5xsfA Crystal structure of the 2-keto-3-deoxy-6-phosphogluconate aldolase of zymomonas mobilis zm4 with 3-phosphoglycerate
50% identity, 94% coverage: 13:215/215 of query aligns to 3:205/209 of 5xsfA
1mxsA Crystal structure of 2-keto-3-deoxy-6-phosphogluconate (kdpg) aldolase from pseudomonas putida. (see paper)
43% identity, 90% coverage: 7:200/215 of query aligns to 5:199/216 of 1mxsA
P00885 2-dehydro-3-deoxy-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase; EC 4.1.2.14 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
43% identity, 90% coverage: 7:200/215 of query aligns to 15:209/226 of P00885
Sites not aligning to the query:
1wa3D Mechanism of the class i kdpg aldolase (see paper)
31% identity, 95% coverage: 11:215/215 of query aligns to 1:200/203 of 1wa3D
2v82A Kdpgal complexed to kdpgal (see paper)
34% identity, 68% coverage: 39:185/215 of query aligns to 26:171/205 of 2v82A
Sites not aligning to the query:
Q6BF16 2-dehydro-3-deoxy-6-phosphogalactonate aldolase; 2-oxo-3-deoxygalactonate 6-phosphate aldolase; 6-phospho-2-dehydro-3-deoxygalactonate aldolase; 6-phospho-2-keto-3-deoxygalactonate aldolase; KDPGal; EC 4.1.2.21 from Escherichia coli (strain K12) (see paper)
34% identity, 68% coverage: 39:185/215 of query aligns to 27:172/205 of Q6BF16
Sites not aligning to the query:
>GFF2758 PGA1_c28010 KHG/KDPG aldolase
MAITPQDASTRARDICQLAPIVPVLVVHDVAHARPLAEALIAGGLPALEVTLRTPAALDV
IAEMAKVKGGVVGAGTLITPEDVTRAVAAGAQFGVSPGATDTLLDAAEAAGLPMLPGAAT
ASEAMRLLDRGYDMLKFFPAEASGGAPALKAIGAPLPQISFCPTGGVSPDNADSYLSLSN
VVCAGGSWVAPAKLVEAGDWDGIRDLARAASQLAR
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory