SitesBLAST
Comparing GFF2803 FitnessBrowser__WCS417:GFF2803 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
28% identity, 81% coverage: 8:444/539 of query aligns to 22:450/456 of 5oqtA
- binding alanine: I38 (= I24), G40 (vs. gap), T41 (vs. gap), G42 (vs. gap), F226 (= F210), A227 (= A211), I229 (≠ L213)
- binding : E24 (≠ Q10), G26 (≠ S12), F28 (≠ M14), D29 (= D15), M32 (≠ F18), A176 (= A156), R177 (≠ I157), A184 (≠ V164), A188 (≠ I168), L192 (= L172), Q294 (≠ R279), V297 (≠ L290)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
28% identity, 81% coverage: 8:444/539 of query aligns to 24:452/458 of 6f34A
- binding arginine: I40 (= I24), G42 (vs. gap), T43 (vs. gap), G44 (vs. gap), E115 (≠ A98), Y116 (≠ F99), A119 (= A109), F228 (= F210), A229 (= A211), I231 (≠ L213), V314 (≠ C305)
- binding cholesterol: W201 (≠ T179), Y202 (≠ F180)
- binding : G28 (≠ S12), F30 (≠ M14), D31 (= D15), M34 (≠ F18), A178 (= A156), R179 (≠ I157), A186 (≠ V164), I187 (≠ F165), A190 (≠ I168), L194 (= L172), Q296 (≠ R279), V299 (≠ L290)
P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
25% identity, 73% coverage: 6:399/539 of query aligns to 25:409/487 of P82251
- V40 (≠ L21) to M: in CSNU; uncertain significance
- IIGSG 43:47 (≠ IFGSG 24:28) binding L-arginine
- I44 (≠ F25) to T: in CSNU; type I; dbSNP:rs121908485
- S51 (≠ A32) to F: in CSNU; uncertain significance
- P52 (≠ A33) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
- A70 (≠ L49) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
- Y99 (= Y78) to H: in CSNU; uncertain significance
- G105 (= G84) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
- W114 (≠ F93) to R: in CSNU; uncertain significance
- I120 (≠ F99) to L: in CSNU; uncertain significance
- T123 (≠ S101) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
- V142 (≠ A124) to A: no effect on amino acid transport activity; dbSNP:rs12150889
- C144 (≠ S126) modified: Interchain (with C-114 in SLC3A1)
- V170 (= V152) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
- A182 (≠ V164) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
- G195 (vs. gap) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
- L223 (≠ M201) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
- A224 (= A202) to V: in CSNU; non-classic type I; dbSNP:rs140873167
- N227 (≠ A205) to D: in CSNU; decreased amino acid transport activity
- W230 (≠ F210) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
- D233 (≠ L213) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
- W235 (≠ L215) mutation to A: Complete loss of amino acid transport activity.
- Q237 (≠ P217) mutation to A: Reduces amino acid transport activity.
- G259 (≠ S239) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
- P261 (≠ L241) to L: in CSNU; types I and III; dbSNP:rs121908486
- S286 (= S271) to F: in CSNU; uncertain significance; dbSNP:rs755135545
- C321 (≠ M310) mutation to S: Does not affect amino acid transport activity.
- A324 (≠ T313) to E: in CSNU; uncertain significance
- V330 (≠ G319) to M: in CSNU; type III; dbSNP:rs201618022
- A331 (≠ W320) to V: in CSNU; non-classic type I; dbSNP:rs768466784
- R333 (≠ Q322) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
- A354 (= A343) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
- S379 (= S369) mutation to A: Markedly reduces amino acid transport activity.
- A382 (≠ L372) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
- W383 (≠ I373) mutation to A: Complete loss of amino acid transport activity.
- Y386 (= Y376) mutation to A: Loss of amino acid transport activity.
- K401 (≠ P391) to E: in CSNU; uncertain significance; dbSNP:rs760264924
Sites not aligning to the query:
- 426 L → P: in CSNU; uncertain significance
- 482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.
P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
27% identity, 63% coverage: 8:347/539 of query aligns to 14:352/489 of P25737
- Y102 (≠ T95) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- W106 (≠ F99) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- K163 (= K166) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- F216 (= F210) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- E222 (≠ T216) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
- E230 (= E224) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
- D275 (≠ G266) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
- D278 (≠ G269) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- 443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- 446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.
7nf6B Ovine b0,+at-rbat heterodimer (see paper)
26% identity, 72% coverage: 10:399/539 of query aligns to 1:381/455 of 7nf6B
6li9B Heteromeric amino acid transporter b0,+at-rbat complex bound with arginine (see paper)
25% identity, 70% coverage: 21:399/539 of query aligns to 11:380/458 of 6li9B
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
24% identity, 76% coverage: 9:417/539 of query aligns to 2:405/433 of 6f2wA
P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
23% identity, 73% coverage: 2:392/539 of query aligns to 3:388/469 of P46349
- G33 (≠ A32) mutation to D: Lack of activity.
- G42 (= G41) mutation to S: Lack of activity.
- G301 (≠ Y309) mutation to V: Lack of activity.
- G338 (vs. gap) mutation to E: Lack of activity.
- F341 (≠ W345) mutation to S: Lack of activity.
Sites not aligning to the query:
- 414 G→R: Lack of activity.
Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
25% identity, 81% coverage: 8:445/539 of query aligns to 37:469/535 of Q9UHI5
- I53 (= I24) binding L-leucine
- Y93 (= Y62) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
- N134 (≠ S101) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
- C154 (≠ S127) modified: Interchain (with C-210 in SLC3A2)
- W174 (vs. gap) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
- F243 (= F210) mutation to A: Abolishes leucine and tryptophan transport activities.
- G246 (≠ L213) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
- V302 (≠ L274) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280
- N395 (≠ L372) binding L-tryptophan; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
- Y396 (≠ I373) mutation to A: Strongly reduces L-leucine uptake activity.
- T402 (≠ A379) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
- R418 (= R395) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
- V460 (≠ F436) to E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization
O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
23% identity, 74% coverage: 2:399/539 of query aligns to 3:386/438 of O34739
- C94 (≠ M91) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
- C141 (= C142) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
- C168 (≠ V173) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
- C291 (= C305) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
Sites not aligning to the query:
- 415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
P60061 Arginine/agmatine antiporter from Escherichia coli (strain K12) (see 3 papers)
28% identity, 64% coverage: 1:344/539 of query aligns to 1:327/445 of P60061
- I23 (= I24) binding agmatine; binding L-arginine
- S26 (= S27) binding L-arginine
- Y93 (≠ T95) mutation to L: Greatly decreased Arg uptake into liposomes.
- A96 (= A98) binding agmatine; binding L-arginine
- C97 (≠ F99) binding agmatine
- N101 (≠ V103) binding agmatine; mutation to A: Vmax for Arg-Agm exchange 1% of wild-type, KM increases 3-fold.; mutation to D: Nearly wild-type Arg-Agm exchange.
- M104 (≠ E106) binding agmatine; mutation to A: 30% decreased affinity for Arg, 50% decreased affinity for Agm.
- W202 (≠ F210) binding L-arginine; mutation to L: Halves Arg uptake into liposomes.
- S203 (≠ A211) binding agmatine
- I205 (≠ L213) binding agmatine; binding L-arginine; mutation to A: About wild-type affinity for Arg and Agm.
- W293 (≠ G306) binding agmatine; mutation W->C,H,L: Loss of Arg-Agm exchange.; mutation W->F,Y: Less than 20% Arg-Agm exchange activity. Vmax 15% of wild-type rate.
Sites not aligning to the query:
- 357 binding L-arginine; S→A: 20% decreased affinity for Arg, 40% decrease affinity for Agm.
P60063 Arginine/agmatine antiporter from Escherichia coli O157:H7 (see 3 papers)
28% identity, 64% coverage: 1:344/539 of query aligns to 1:327/445 of P60063
- N22 (≠ A23) mutation to A: No change in antiport activity, 6-fold higher affinity for Arg.
- I23 (= I24) binding L-arginine
- GSG 25:27 (= GSG 26:28) Helix-breaking GSG motif TM1
- S26 (= S27) binding L-arginine; mutation to K: 5% Agm antiport.
- G27 (= G28) binding L-arginine
- Y74 (≠ V76) mutation to A: 50% antiport activity at pH 6.0, 10-fold higher than wild-type antiport activity at pH 7.5, i.e. loss of pH-dependence of substrate transport. No change in binding of Arg or Agm.; mutation Y->C,H,L,M,Q,S: Loss of pH-dependence of substrate transport.; mutation to F: Approximately wild-type antiport.
- Y87 (= Y89) mutation to A: Markedly reduced binding affinity for Agm but not for Arg. 50% Agm antiport.
- Y93 (≠ T95) mutation to A: Reduced binding affinity for Arg, no binding to Agm. 25% Agm antiport.; mutation to K: Almost no binding to both Arg and Agm. 5% Agm antiport.
- A96 (= A98) binding L-arginine
- C97 (≠ F99) binding L-arginine
- N101 (≠ V103) binding L-arginine
- W202 (≠ F210) Periplasmic (proximal) gate; binding L-arginine
- I205 (≠ L213) binding L-arginine
- GVESA 206:210 (≠ GLTPI 214:218) Helix-breaking GVESA motif TM6
- E208 (≠ T216) mutation E->A,D: 5-10% Agm antiport.
- W293 (≠ G306) binding L-arginine
Sites not aligning to the query:
- 337 F→A: Severely decreased antiport.
- 357 binding L-arginine
- 365 Y→A: Markedly weakened binding to Arg but not to Agm. 5% Agm antiport.
Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
25% identity, 81% coverage: 8:445/539 of query aligns to 36:468/531 of Q9QXW9
- Y130 (vs. gap) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
- N133 (≠ S101) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
- F242 (= F210) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.
P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
23% identity, 72% coverage: 6:392/539 of query aligns to 10:390/457 of P15993
- Y103 (≠ F99) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.
5j4nA Crystal structure of the l-arginine/agmatine antiporter adic in complex with agmatine at 2.6 angstroem resolution (see paper)
27% identity, 62% coverage: 9:344/539 of query aligns to 4:323/437 of 5j4nA
3l1lA Structure of arg-bound escherichia coli adic (see paper)
27% identity, 62% coverage: 9:344/539 of query aligns to 2:310/423 of 3l1lA
Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
24% identity, 87% coverage: 8:476/539 of query aligns to 47:499/507 of Q01650
- Y117 (≠ V76) mutation to A: Strongly decreased leucine transport activity.
- C164 (vs. gap) modified: Interchain (with C-210 in SLC3A2)
- D223 (≠ F178) to V: in dbSNP:rs17853937
- N230 (= N185) to K: in dbSNP:rs1060250
- A246 (= A202) mutation to V: Nearly abolishes leucine transport activity.
- F252 (= F210) mutation to A: Nearly abolishes leucine transport activity.
- W257 (≠ L215) mutation to A: Nearly abolishes leucine transport activity.
- N258 (≠ T216) mutation to A: Decreased leucine transport activity.; mutation to D: Nearly abolishes leucine transport activity.
- Y259 (≠ P217) mutation to A: Strongly decreased leucine transport activity.
- E303 (= E261) mutation to K: Decreased leucine transport activity.
- P375 (= P342) mutation to L: Nearly abolishes leucine transport activity.
Sites not aligning to the query:
- 483:507 mutation Missing: Nearly abolishes leucine transport activity.
7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
25% identity, 71% coverage: 21:400/539 of query aligns to 10:383/458 of 7cmiB
7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
25% identity, 71% coverage: 21:400/539 of query aligns to 10:383/458 of 7cmhB
7b00A Human lat2-4f2hc complex in the apo-state (see paper)
25% identity, 71% coverage: 21:400/539 of query aligns to 10:383/457 of 7b00A
Sites not aligning to the query:
Query Sequence
>GFF2803 FitnessBrowser__WCS417:GFF2803
MSAQGKFKKQLSLMDLTFIGLGAIFGSGWLFAASHVSAIAGPAGIISWLIGGFAVLLLGI
VYCELGAALPRAGGVVRYPVYSHGPLLGYLMGFITLIAFSSLVAIEVVASRQYAAAWFPE
LTKAGSSDPTTLGWLVQFALLCLFFVLNYRSVKTFAIANNLVSVFKFIVPLLVIGVLFTF
FKPANFQVQGFAPFGLSGIEMAVSAGGVIFAYLGLTPIISVASEVKNPQRTIPIALILSV
LLSTAIYVLLQTAFLGGVPTEMLANGWAGISKELALPYRDIALALGVGWLAYLVVADAVI
SPSGCGNIYMNATPRVVYGWAQTGTFFKIFTRIDEKSGIPRPALWLTFALSVFWTLPFPS
WEALINVVSAALILSYAVAPVTVAALRRNAPQMARPFRVKGMAVLGPLSFIIAALIVYWS
GWNTVSWLLGLQILMFVVYLLCARWVPTAHLNIKQQVRSSAWLIGFYAVTILLSKLGSFG
GIGVIGHPLDTIVVAACALGIYYWGAATGVPAHLVRLEHEADESEAVDEPHAPLTPATY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory