SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2832 FitnessBrowser__Phaeo:GFF2832 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

8gxkB Pseudomonas jinjuensis n-acetyltransferase (see paper)
35% identity, 95% coverage: 5:187/193 of query aligns to 3:182/188 of 8gxkB

query
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8gxkB

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binding coenzyme a: M40 (≠ T41), T93 (= T98), W94 (= W99), H100 (≠ Q105), G101 (≠ R106), T129 (= T134), N133 (= N138), L134 (≠ R139), R135 (≠ Q140), S136 (= S141), A139 (= A144), K142 (≠ R147)

1yreB Hypothetical protein pa3270 from pseudomonas aeruginosa in complex with coa
35% identity, 95% coverage: 4:187/193 of query aligns to 2:182/187 of 1yreB

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binding coenzyme a: M40 (≠ T41), T93 (= T98), W94 (= W99), H100 (≠ Q105), G101 (≠ R106), N105 (= N110), R135 (≠ Q140), G138 (≠ A143)

8gxfB Pseudomonas flexibilis gcn5 family acetyltransferase (see paper)
34% identity, 95% coverage: 4:187/193 of query aligns to 2:182/187 of 8gxfB

query
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binding coenzyme a: T93 (= T98), H100 (≠ Q105), G101 (≠ R106), K109 (= K114), T129 (= T134), R135 (≠ Q140)

6c37A Mycobacterium smegmatis rimj in complex with coa-disulfide
30% identity, 68% coverage: 63:193/193 of query aligns to 77:205/209 of 6c37A

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6c37A

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binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: G110 (≠ S97), Y111 (≠ T98), W112 (= W99), V113 (≠ Y100), T118 (≠ Q105), G119 (≠ R106), G121 (= G108), A123 (≠ N110), T124 (= T111), T146 (≠ R133), N151 (= N138), T152 (≠ R139), P153 (≠ Q140), S154 (= S141), V157 (≠ A144), H160 (≠ R147), R205 (= R193)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 42

6c32A Mycobacterium smegmatis rimj with accoa
30% identity, 68% coverage: 63:193/193 of query aligns to 77:205/209 of 6c32A

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6c32A

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binding acetyl coenzyme *a: G110 (≠ S97), Y111 (≠ T98), W112 (= W99), V113 (≠ Y100), T118 (≠ Q105), G119 (≠ R106), G121 (= G108), A123 (≠ N110), T124 (= T111), T146 (≠ R133), N151 (= N138), T152 (≠ R139), P153 (≠ Q140), S154 (= S141), V157 (≠ A144), H160 (≠ R147), R205 (= R193)

Query Sequence

>GFF2832 FitnessBrowser__Phaeo:GFF2832
MWASPLPLTGKTVSLTPLLPDHASDLAEAAADGDLHRLWYTTVPAPQDIGTEINRRLALQ
DSGSMAPFAILDSTGRAVGMTTYMNIDAANRRLEIGSTWYRTSVQRSGINTECKFLLLSH
AFETLNAIAVEFRTHRLNRQSRAAIERLGAQLDGILRAHMILANGTQRDTAVYSITASEW
PTIRAHLLHLLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory