SitesBLAST

R26 (= R21) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (≠ T49) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F82) mutation to L: No effect on phenylalanine transport activity.
F90 (= F85) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ G87) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y89) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L90) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ R93) mutation to L: No effect on phenylalanine transport activity.
F101 (= F96) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W100) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y102) mutation to L: No effect on phenylalanine transport activity.
W108 (= W103) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W106) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D113) mutation E->G,L,V,N: Loss of activity.
K168 (= K163) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E224) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R250) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P339) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (= P442) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
36% identity, 97% coverage: 6:456/465 of query aligns to 3:448/457 of P15993

query
sites
P15993

S

E

G

G

L

Q

Y

Q

E

H

K

G

P

E

A

Q

L

L

Q

K

R

R

T

G

L

L

S

K

N

N

R

R

H

H

I

I

Q

Q

L

L

M

I

A

A

M

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

S

G

A

K

S

I

V

I

I

A

Q

L

S

S

A

G

G

T

P

S

G

I

I

L

L

I

G

Y

Y

M

A

I

I

I

A

G

G

L

F

F

I

V

A

Y

F

F

L

V

I

M

M

R

R

A

Q

M

L

G

G

E

E

L

M

L

V

S

E

N

E

L

P

N

V

F

A

K

G

S

S

F

F

A

S

D

H

F

F

A

A

G

Y

A

K

Y

Y

L

W

G

G

P

S

R

F

A

A

A

G

F

F

F

A

L

S

G

G

W

W

S

N

Y

Y

W

W

L

V

S

L

W
x
Y

S

V

V

L

A

V

V

A

I

M

G

A

D

E

A

L

V

T

V

A

V

V

G

G

G

K

F

Y

F

I

Q

Q

Y

F

W

W

F

Y

P

P

H

E

V

I

P

P

A

T

W

W

M

V

P

S

A

A

V

A

G

V

M

F

L

F

L

V

T

V

L

I

F

N

A

A

L

I

N

N

V

L

L

T

T

N

V

V

R

K

L

V

F

F

G

G

E

E

V

M

E

E

F

F

W

W

F

F

A

A

I

I

K

K

L

V

I

I

A

A

V

V

L

V

T

A

L

M

I

I

G

I

V

F

S

G

G

G

V

W

L

L

I

L

A

-

S

-

F

F

V

S

S

G

P

N

T

G

G

G

V

P

T

Q

A

A

S

T

F

V

T

S

H

N

L

L

L

W

D

D

P

Q

Q

G

A

G

A

F

F

L

P

P

N

H

G

G

L

F

F

T

G

G

F

L

F

V

A

M

G

M

F

M

Q

A

M

I

A

I

I

M

F

F

S

S

F

F

A

G

G

G

T

L

E

E

L

L

I

V

G

G

T

I

A

T

A

A

E

E

T

A

R

D

A

N

P

P

E

E

K

Q

T

S

L

I

P

P

K

K

A

A

I

T

N

N

S

Q

I

V

P

I

L

Y

R

R

I

I

I

L

L

I

F

F

Y

Y

V

I

L

G

A

S

L

L

A

A

C

V

I

L

A

S

V

L

T

M

S

P

W

W

Q

T

H

R

V

V

S

T

P

A

S

D

K

T

S

S

P

P

F

F

V

V

E

L

L

I

F

F

L

H

V

E

A

L

G

G

F

D

P

T

A

F

A

V

A

A

G

N

I

A

V

L

N

N

F

I

V

V

L

L

T

T

S

A

A

A

A

L

S

S

S

V

A

Y

N

N

S

S

G

C

V

V

F

Y

S

C

A

N

S

S

R

R

M

M

L

L

F

F

G

G

L

L

A

A

D

Q

L

Q

G

G

D

N

A

A

P

P

G

K

I

A

F

L

R

A

R

S

L

V

S

D

K

K

S

R

S

G

V

V

P

P

F

V

I

N

S

T

L

I

A

L

F

V

T

S

T

A

L

L

L

V

M

T

L

A

L

L

G

C

L

V

V

L

L

I

L

N

F

Y

V

L

V

A

P

P

E

E

V

S

M

A

T

F

A

G

F

L

T

L

I

M

V

A

S

L

T

V

V

V

S

S

A

A

I

L

L

V

V

I

I

-

F

-

T

N

W

W

S

A

T

M

I

I

L

S

A

L

S

A

Y

H

I

M

A

K

Y

F

R

R

K

R

K

A

R

K

P

Q

D

E

L

Q

H

G

A

V

K

V

S

T

L

R

Y

F

K

P

M

A

P

L

G

L

G

Y

V

P

L

L

M

G

A

N

W

W

F

I

S

C

L

L

A

L

F

F

L

M

A

A

F

A

V

V

L

L

G

V

L

I

L

M

A

L

L

M

R

T

P

P

D

G

T

M

R

A

L

I

A

S

L

V

C

Y

V

L

M

I

P

P

A

V

W

W

F

L

I

I

W

V

L

L

W

G

I

I

A

G

Y

Y

P

L

F

F

S

K

R

E

Y103 (≠ W106) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
34% identity, 85% coverage: 11:404/465 of query aligns to 5:394/469 of P46349

query
sites
P46349

K

Q

P

S

A

G

L

L

Q

K

R

K

T

E

L

L

S

K

N

T

R

R

H

H

I

M

Q

T

L

M

M

I

A

S

M

I

G

A

G

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

M

V

G
|
G

S

S

G

G

K

S

I

V

I

I

A

H

L

S

S

T

G
|
G

T

P

S

G

I

A

I

V

L

V

I

S

Y

Y

M

A

I

L

I

A

G

G

L

L

F

L

V

V

Y

I

F

F

V

I

M

M

R

R

A

M

M

L

G

G

E

E

L

M

L

S

L

A

S

V

N

N

L

P

N

T

F

S

K

G

S

S

F

F

A

S

D

Q

F

Y

A

A

G

H

A

D

Y

A

L

I

G

G

P

P

R

W

A

A

A

G

F

F

F

T

L

I

G

G

W

W

S

L

Y

Y

W

W

L

F

S

F

W

W

S

V

V

I

A

V

V

I

I

A

G

I

D

E

A

A

V

I

V

A

V

G

G

A

G

G

F

I

Q

Q

Y

Y

W

W

F

F

P

H

H

D

V

I

P

P

A

L

W

W

M

L

P

T

A

S

V

L

G

I

M

L

L

T

L

I

T

V

L

L

F

T

A

L

L

T

N

N

V

V

L

Y

T

S

V

V

R

K

L

S

F

F

G

G

E

E

V

F

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K

K

L

V

I

V

A

T

V

I

L

I

T

A

L

F

I

L

G

-

V

-

S

-

G

-

V

-

L

I

I

V

A

G

S

F

S

A

F

F

V

I

-

F

-

G

-

F

S

A

P

P

T

G

G

S

V

E

T

P

A

V

S

G

F

F

T

S

H

N

L

L

L

T

D

G

P

K

Q

G

A

G

A

F

F

F

P

P

N

E

G

G

L

I

F

S

G

S

F

V

F

L

A

L

G

G

F

I

Q

V

M

V

A

V

I

I

F

F

S

S

F

F

A

M

G

G

T

T

E

E

L

I

I

V

G

A

T

I

A

A

A

G

E

E

T

T

R

S

A

N

P

P

E

I

K

E

T

S

L

V

P

T

K

K

A

A

I

T

N

R

S

S

I

V

P

V

L

W

R

R

I

I

L

V

F

F

Y

Y

V

V

L

G

A

S

L

I

A

A

C

I

I

V

A

A

V

L

T

L

S

P

W

W

Q

N

H

S

V

A

S

N

P

I

S

L

K

E

S

S

P

P

F

F

V

V

E

A

L

V

F

L

L

E

V

H

A

I

G

G

F

V

P

P

A

A

G

Q

I

I

V

M

N

N

F

F

V

I

V

V

L

L

T

T

S

A

A

V

A

L

S

S

S

C

A

L

N

N

S

S

G
|
G

V

L

F

Y

S

T

A

T

S

S

R

R

M

M

L

L

F

Y

G

S

L

L

A

A

D

E

L

R

G

N

D

E

A

A

P

P

G

R

I

R

F

F

R

M

R

K

L

L

S

S

K

K

S

K

S

G

V

V

P

P

F

V

I

Q

S

A

L

I

A

V

F

A

T
x
G

T

T

L

F

L
x
F

M

S

L

Y

L

I

G

A

L

V

V

V

L

M

L

N

F

Y

V

F

V

S

P

P

E

D

V

-

M

-

T

T

A

V

F

F

T

L

I

F

V

L

S

V

T

N

V

S

S

S

A

G

I

A

L

I

V

A

I

L

F

L

T

V

W

Y

S

L

T

V

I

I

L

A

A

V

S

S

Y

Q

I

L

A

K

Y

M

R

R

K

K

R

L

P

E

D

K

L

T

H

N

A

P

K

E

S

A

L

L

G33 (= G39) mutation to D: Lack of activity.
G42 (= G48) mutation to S: Lack of activity.
G301 (= G309) mutation to V: Lack of activity.
G338 (≠ T346) mutation to E: Lack of activity.
F341 (≠ L349) mutation to S: Lack of activity.

Sites not aligning to the query:

414 G→R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
36% identity, 86% coverage: 10:408/465 of query aligns to 9:411/489 of P25737

query
sites
P25737

E

E

K

A

P

P

A

G

L

L

Q

R

R

R

T

E

L

L

S

K

N

A

R

R

H

H

I

L

Q

T

L

M

M

I

A

A

M

I

G

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

M

V

G

A

S

S

G

G

K

A

I

T

I

I

A

S

L

Q

S

A

G

G

-

P

T

G

S

G

I

A

I

L

L

L

I

S

Y

Y

M

M

I

L

I

I

G

G

L

L

F

M

V

V

Y

Y

F

F

V

L

M

M

R

T

A

S

M

L

G

G

E

E

L

L

L

A

S

Y

N

M

L

P

N

V

F

S

K

G

S

S

F

F

A

A

D

T

F

Y

A

G

G

Q

A

N

Y

Y

L

V

G

E

P

E

R

G

A

F

A

G

F

F

F

A

L

L

G

G

W

W

S

N

Y
|
Y

W

W

L

Y

S

N

W
|
W

S

A

V

V

A

T

V

I

I

A

G

V

D

D

A

L

V

V

V

A

G

Q

G

L

F

V

F

M

Q

S

Y

W

W

W

F

F

P

P

H

D

V

T

P

P

A

G

W

W

M

I

P

W

A

S

V

A

G

L

M

F

L

L

L

G

T

V

L

I

F

F

A

L

L

L

N

N

V

Y

L

I

T

S

V

V

R

R

L

G

F

F

G

G

E

E

V

A

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K
|
K

L

V

I

T

A

T

V

V

L

I

T

V

L

F

I

I

G

I

V

V

S

G

G

V

V

L

L

M

I

I

A

I

S

G

S

I

F

F

V

K

S

G

P

A

T

Q

G

-

V

-

T

P

A

A

S

G

F

W

T

S

H

N

L

W

L

T

D

I

P

G

Q

E

A

A

A

P

F

F

P

A

N

G

G

G

L

-

F

F

G

A

F

A

F

M

A

I

G

G

F

V

Q

A

M

M

A

I

I

V

-

G

F
|
F

S

S

F

F

A

Q

G

G

T

T

E
|
E

L

L

I

I

G

G

T

I

A

A

A

G

E
|
E

T

S

R

E

A

D

P

P

E

A

K

K

T

N

L

I

P

P

K

R

A

A

I

V

N

R

S

Q

I

V

P

F

L

W

R

R

I

I

I

L

L

L

F

F

Y

Y

V

V

L

F

A

A

L

I

A

L

C

I

I

I

I

S

A

L

V

I

T

I

S

P

W

Y

Q

T

H

D

V

P

S

S

-

L

-

R

-

N

-
x
D

-

V

-

K

-
x
D

P

I

S

S

K

V

S

S

P

P

F

F

V

T

E

L

L

V

F

F

L

Q

V

H

A

A

G

G

F

L

P

L

A

S

A

A

G

A

I

V

V

M

N

N

F

A

V

V

V

I

L

L

T

T

S

A

A

V

A

L

S

S

S

A

A

G

N

N

S

S

G

G

V

M

F

Y

S

A

A

S

S

T

R

R

M

M

L

L

F

Y

G

T

L

L

A

A

D

C

L

D

G

G

D

K

A

A

P

P

G

R

I

I

F

F

R

A

R

K

L

L

S

S

K

R

S

G

V

V

P

P

F

R

I

N

S

A

L

L

A

Y

F

A

T

T

L

V

L

I

M

A

L

-

G

G

L

L

V

C

L

F

L

L

F

T

V

S

V

M

P

F

E

G

V

N

M

Q

T

T

A

V

F

Y

T

L

I

W

V

L

S

L

T

N

V

T

S

S

A

G

I

M

L

T

V

G

I

F

F

I

T

A

W

W

S

L

T

G

I

I

L

A

A

I

S

S

Y

H

I

Y

A

R

Y

F

R

R

K

R

K

G

R

Y

P

V

D

-

L

L

H

Q

A

G

K

H

S

D

L

I

Y

N

K

D

M

L

P

P

Y102 (= Y102) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W106) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K163) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F218) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E224) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E232) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 83% coverage: 10:393/465 of query aligns to 76:466/587 of Q9URZ4

query
sites
Q9URZ4

E

D

K

G

P

T

A

A

L

L

Q

K

R

R

T

S

L

L

S

K

N

S

R

R

H

H

I

M

Q

Q

L

M

M

I

A

S

M

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

M

V

G

G

S

S

G

G

K

S

I

S

I

L

A

A

L

D

S

G

G

G

-

P

T

A

S

S

I

V

I

I

L

I

I

N

Y

Y

M

S

I

L

I

I

G

G

L

I

F

M

V

M

Y

F

F

F

V

I

M

V

R

Y

A

A

M

L

G

G

E

E

L

M

L

A

L

V

S

A

N

Y

L

P

N

V

F

A

K

G

S

G

F

F

A

N

D

T

F

Y

A

A

G

T

A

R

Y

F

L

I

G

D

P

P

R

A

A

W

A

G

F

F

F

A

L

V

G

S

W

W

S

N

Y

Y

W

F

L

I

S

N

W

Y

S

F

V

V

A

T

V

F

I

P

G

L

D

E

A

L

V

T

V

C

G

A

G

I

F

T

F

F

Q

R

Y

Y

W

W

F

T

P

D

-

I

H

N

V

S

P

A

A

A

W

W

M

I

P

S

A

I

V

-

G

-

M

F

L

L

L

V

T

V

L

I

F

I

A

I

L

V

N

N

V

L

L

F

T

G

V

V

R

R

L

A

F

Y

G

G

E

E

V

V

E

E

F

F

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I

F

L

A

S

I

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I

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K

K

L

V

I

V

A

A

V

T

L

F

T

G

L

F

I

I

G

-

V

I

S

L

G

A

V

I

L

I

I

I

A

N

S

C

S

G

F

G

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V

S

-

P

P

T

T

G

D

V

H

T

R

A

G

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Y

F

I

T

G

H

G

L

S

L

I

D

I

P

K

Q

H

A

K

A

P

F

F

P

R

N

H

G

G

L

F

F

K

G

G

F

F

F

C

A

S

G

V

F

F

Q

T

M

T

A

A

I

A

F

F

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S

F

F

A

S

G

G

T

T

E

E

L

V

I

I

G

G

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L

A

A

E

E

T

V

R

D

A

N

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P

E

Q

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K

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A

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L

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P

K

H

A

A

I

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N

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S

Q

I

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P

F

L

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R

R

I

I

I

A

L

I

F

F

Y

Y

V

V

L

V

A

S

L

L

A

I

C

L

I

I

I

G

A

L

V

L

T

I

S

S

W

P

Q

D

-

D

-

P

-

N

-

L

-

M

-

G

H

N

V

G

S

S

P

T

S

S

K

V

S

S

P

P

F

F

V

V

E

L

L

A

F

I

L

K

V

E

A

A

G

N

F

I

P

K

A

G

A

L

A

P

G

S

I

V

V

F

N

N

F

A

V

V

V

I

L

I

T

I

S

S

A

V

S

S

S

V

A

T

N

N

S

S

G

S

V

T

F

Y

S

T

A

A

S

G

R

R

M

T

L

L

F

H

G

G

L

M

A

A

D

N

L

L

G

K

D

Q

A

A

P

P

G

S

I

F

F

F

R

K

R

Y

L

T

S

D

K

R

S

L

S

G

V

R

P

P

F

L

I

L

S

A

L

M

A

-

F

-

T

-

L

I

L

V

M

V

L

L

G

F

L

G

V

F

L

F

L

A

F

Y

V

I

V

N

P

E

E

A

V

D

M

K

T

N

A

G

F

N

T

D

I

V

V

S

S

D

T

T

V

V

-

F

-

N

-

W

-

L

-

A

-

L

S

S

A

G

I

L

L

S

V

N

I

F

F

F

T

T

W

W

S

G

T

S

I

I

L

C

A

L

S

C

Y

H

I

I

A

I

Y

F

R

R

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 73% coverage: 3:343/465 of query aligns to 73:427/590 of P04817

query
sites
P04817

T

D

T

E

T

D

S

E

G

G

L

E

Y

V

E

Q

K

N

P

A

A

E

L

V

Q

K

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R

T

E

L

L

S

K

N

Q

R

R

H

H

I

I

Q

G

L

M

M

I

A

A

M

L

G

G

A

T

I

I

G

G

T

T

G

G

L

L

F

F

M

I

G

G

S

L

G

S

K

T

I
x
P

I

L

A

T

L

N

S

A

G

G

T

P

S

V

I

G

I

A

L

L

I

I

-

S

Y

Y

M

L

I

F

I

M

G

G

L

S

F

L

V

A

Y

Y

F

S

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V

M

T

R

Q

A

S

M

L

G

G

E

E

L

M

L

A

L

T

S

F

N

I

L
x
P

N
x
V

F

T

K

S

S
|
S

F

F

A

T

D

V

F

F

A

S

G

Q

A

R

Y

F

L

L

G

S

P

P

R

A

A

F

A

G

F

A

L

N

G

G

W

Y

S

M

Y
|
Y

W

W

L

F

S

S

W

W

S

A

V

I

A

T

V

F

I

A

G

L

D

E

A

L

V

S

V

V

G

G

G

Q

F

V

F

I

Q

Q

Y

F

W

W

F

T

P

Y

H

K

V

V

P

P

-

L

-

A

A

A

W

W

M

I

P

S

A

I

V

F

G

W

M

V

L

I

T

T

L

I

F

-

A

-

L

M

N

N

V

L

L

F

T

P

V

V

R

K

L

Y

F

Y

G

G

E

E

V

F

E

E

F

F

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W

F

V

A

A

I

S

I

I

K

K

L

V

I

L

A

A

V

I

L

I

T

G

-

F

L

L

I

I

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Y

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F

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C

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M

L

V

I

C

A

G

S

A

S

G

F

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V

T

S

G

P

P

T

V

G

G

V

-

T

-

A

-

S

-

F

F

T

R

H

Y

L

W

L

R

D

N

P

P

Q

G

A

A

A

W

F

G

P

P

-

G

-

I

-

S

-

K

-

D

-

K

-

N

N

E

G

G

L

R

F

F

-

L

G

G

F

W

F

V

A

S

G

S

F

L

Q

I

M

N

A

A

I

A

F

F

S

T

F

F

A

Q

G

G

T

T

E

E

L

L

I

V

G

G

T

I

A

T

A

A

A
x
G

E

E

T

A

R

A

A

N

P
|
P

E

R

K

K

T

S

L

V

P

P

K

R

A

A

I

I

N

K

S

K

I

V

P

V

L

F

R

R

I

I

I

L

L

T

F

F

Y

Y

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I

L

G

A

S

L

L

A

L

C

F

I

I

I

G

A

L

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L

T

V

S

P

W

Y

Q

N

H

D

V

P

S

K

-

L

-

T

-

Q

-

S

-
x
T

-
x
S

-
x
Y

P

V

S

S

K

T

S

S

P

P

F

F

V

I

E

I

L

A

F

I

L

E

V

N

A

S

G

G

F

T

P

K

A

V

A

L

A

P

G

H

I

I

V

F

N

N

F

A

V

V

V

I

L

L

T

T

S

T

A

I

S

S

S

A

A

A

N

N

S

S

G

N

V

I

F

Y

S

V

A

G

S

S

R

R

M

I

L

L

F

F

G

G

L

L

A

S

D

K

L

N

G

K

D

L

A

A

P

P

G

K

I

F

F

L

R

S

R

R

L

T

S

T

K

K

S

G

V

V

P

P

F

Y

I

I

S

A

L

V

P113 (≠ I43) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (≠ L77) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ N78) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S81) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y102) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A231) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P236) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.

Sites not aligning to the query:

451 W→C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; W→L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; W→S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
461 F→S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
30% identity, 82% coverage: 14:394/465 of query aligns to 86:476/602 of P19145

query
sites
P19145

L

L

Q

K

R

H

T

H

L

L

S

K

N

N

R

R

H

H

I

L

Q

Q

L

M

M

I

A

A

M

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

S

G

G

K

T

I

A

I

L

A

R

L

T

S

G

G

G

T

P

S

A

I

S

I

L

L

L

I

I

-

G

Y

W

M

G

I

S

I

T

G

G

L

T

F

M

V

I

Y

Y

F

A

V

M

M

V

R

M

A

A

M

L

G

G

E

E

L

L

L

A

L

V

S

I

N

F

L

P

N

I

F

S

K

G

S

G

F

F

A

T

D

T

F

Y

A

A

G

T

A

R

Y

F

L

I

G

D

P

E

R

S

A

F

A

G

F

Y

F

A

L

N

G

N

W

F

S

N

Y

Y

W

M

L

L

S

Q

W

W

S

L

V

V

A

V

V

L

I

P

G

L

D

E

A

I

V

V

V

S

V

A

G

S

G

I

F

T

F

V

Q

N

Y

F

W

W

F

G

P

T

H

D

V

-

P

P

A

K

W

Y

M

R

P

D

A

G

V

F

G

V

M

A

L

L

-

F

-

W

L

L

T

A

L

I

F

V

A

I

L

I

N

N

V

M

L

F

T

G

V

V

R

K

L

G

F

Y

G

G

E

E

V

A

E

E

F

F

W

V

F

F

A

S

I

F

I

I

K

K

L

V

I

I

A

T

V

V

L

V

T

G

L

F

I

I

G

I

V

L

S

G

G

I

V

I

L

L

I

N

A

C

S

G

F

G

V

P

S

T

P

G

T

G

G

Y

V

I

T

G

A

G

S

K

F

Y

T

W

H

H

L

-

D

D

P

P

Q

G

A

A

-

F

-

A

-

G

A

D

F

T

P

P

N

G

G

A

L

K

F

F

-

K

G

G

F

V

F

C

A

S

G

V

F

F

Q

V

M

T

A

A

I

A

F

F

S

S

F

F

A
|
A

G

G

T

S

E

E

L

L

I

V

G

G

T

L

A

A

A

S

E

E

T

S

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V

A

E

P

P

E

R

K

K

T

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P

P

K

K

A

A

I

A

N

K

S

Q

I

V

P

F

L

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R

R

I

I

I

T

L

L

F

F

Y

Y

V

I

L

L

A

S

L

L

A

L

C

M

I

I

I

G

A

L

V

L

T

V

S

P

W

Y

Q

N

H

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

V

V

S

D

P

A

S

A

K

A

S

S

P

P

F

F

V

V

E

I

L

A

F

I

L

K

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T

A

H

G

G

F

I

P

K

A

G

A

L

A

P

G

S

I

V

V

V

N

N

F

V

V

V

V

I

L

L

T

I

S

A

A

V

A

L

S

S

S

V

A

G

N

N

S

S

G

A

V

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Y

S

A

A

C

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S

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M

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M

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G

A

L

L

A

A

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L

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D

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A

L

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P

G

E

I

I

F

F

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L

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R

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G

L

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A

A

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S

L

A

L

F

M

G

L

L

L

I

G

A

L

F

V

V

L

A

L

-

F

-

V

A

V

S

P

K

E

K

V

E

M

G

T

E

A

V

F

F

T

N

I

W

V

L

S

L

T

A

V

L

S

S

A

G

I

L

L

S

V

S

I

L

F

F

T

T

W

W

S

G

T

G

I

I

L

C

A

I

S

C

Y

H

I

I

A

R

Y

F

R

R

K

K

A297 (= A221) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 90% coverage: 10:429/465 of query aligns to 141:571/663 of P48813

query
sites
P48813

E

K

K

Q

P

E

A

N

L

L

Q

K

R

K

T

S

L

I

S

K

N

P

R

R

H

H

I

T

Q

V

L

M

M

M

A

S

M

L

G

G

G

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

N

G

S

K

K

I

V

I

L

A

N

L

N

S

A

G

G

T

P

S

G

I

G

I

L

L

I

I

I

-

G

Y

Y

M

A

I

I

I

M

G

G

L

S

F

C

V

V

Y

Y

F

C

V

I

M

I

R

Q

A

A

M

C

G

G

E

E

L

L

L

A

L

V

S

I

N

-

L

-

N

-

F

-

K

-

S

-

F

Y

A

S

D

D

F

L

A

I

G

G

A

G

Y

F

-

N

-

T

-

Y

-

P

-

L

-

F

-

L

L

V

G

D

P

P

R

A

A

L

A

G

F

F

F

S

L

V

G

A

W

W

S

L

Y

F

W

C

L

L

S

Q

W

W

S

L

V

C

A

V

V

C

I

P

G

L

D

E

A

L

V

V

V

T

V

A

G

S

G

M

F

T

F

I

Q

K

Y

Y

W

W

F

T

P

T

H

S

V

V

P

N

A

P

W

D

M

V

P

F

A

V

V

V

G

I

M

F

L

Y

L

V

T

L

L

I

F

V

A

V

L

I

N

N

V

V

L

F

T

G

V

A

R

K

L

G

F

Y

G

A

E

E

V

A

E

D

F

F

W

F

F

F

A

N

I

C

K

K

L

I

I

L

A

M

V

I

L

V

T

G

L

F

I

F

G

-

V

I

S

L

G

A

V

I

L

I

I

I

A

D

S

C

S

G

F

G

V

A

S

G

P

T

T

D

G

G

V

Y

T

I

A

G

S

S

F

-

T

K

H

Y

L

W

L

R

D

D

P

P

-

G

-

A

-

F

Q

R

A

G

A

D

F

T

P

P

N

I

G

Q

L

R

F

F

-

K

G

G

F

V

A

A

G

T

F

F

Q

V

M

T

A

A

I

A

F

F

S

A

F

F

A

G

G

M

T

S

E

E

L

Q

I

L

G

A

T

M

A

T

A

A

A

S

E

E

T

Q

R

S

A

N

P

P

E

R

K

K

T

A

L

I

P

P

K

S

A

A

I

A

N

K

S

K

I

M

P

I

L

Y

R

R

I

I

I

L

L

F

F

V

Y

F

V

L

L

A

A

S

L

L

A

T

C

L

I

V

-

G

-

F

-

L

I

V

A

P

V

Y

T

T

S

S

W

D

Q

Q

H

L

V

L

-

G

-

A

-

G

S

S

P

A

S

T

K

K

-

A

S

S

P

P

F

Y

V

V

E

I

L

A

F

V

L

S

V

S

A

H

G

G

F

V

P

R

A

V

A

P

G

H

I

F

V

I

N

N

F

A

V

V

V

I

L

L

T

L

S

S

A

V

A

L

S

S

S

V

A

A

N

N

S

G

G

A

V

F

F

Y

S

T

A

S

S

S

R

R

M

I

L

L

F

M

G

S

L

L

A

A

D

K

L

Q

G

G

D

N

A

A

P

P

G

K

I

C

F

F

R

D

R

Y

L

I

S

D

K

R

S

E

S

G

V

R

P

P

F

A

I

A

S

A

L

M

A

L

F

V

T

S

T

A

L

L

L

F

M

G

L

V

L

I

G

A

L

F

V

C

L

A

L

S

F

S

V

K

P

E

E

E

V

-

M

-

T

D

A

V

F

F

T

T

I

W

V

L

S

L

T

A

V

I

S

S

A

G

I

L

L

S

V

Q

I

L

F

F

T

T

W

W

S

I

T

T

I

I

L

C

A

L

S

S

Y

H

I

I

A

R

Y

F

R

R

K

R

K

A

R

M

P

K

D

-

L

V

H

Q

A

G

K

R

S

S

L

L

-

G

-

E

-

V

-

G

Y

Y

K

K

M

S

P

Q

G

V

G

G

V

V

L

-

M

-

A

-

W

W

F

G

S

S

-

A

L

Y

A

A

F

V

L

L

A

M

F

M

V

V

L

L

G

A

L

L

L

I

A

A

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 86% coverage: 10:408/465 of query aligns to 273:746/852 of Q03770

query
sites
Q03770

E

R

K

K

P

Y

A

H

L

I

Q

Q

R

R

T

K

L

L

S

K

N

V

R

R

H

H

I

I

Q

Q

L

M

M

L

A

S

M

I

G

G

G

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

M

L

G

T

S

S

G

G

K

K

I

A

I

F

A

S

L

I

S

A

G

G

T

P

-

F

S

G

I

T

I

L

L

L

I

G

Y

F

M

G

I

L

I

T

G

G

L

S

F

I

V

I

Y

L

F

A

V

T

M

M

R

L

A

S

M

F

G

T

E

E

L

L

L

S

L

T

S

L

N

I

L

P

N

V

F

S

K

S

S

G

F

F

A

S

D

G

F

L

A

A

G

S

A

R

Y

F

L

V

G

E

P

D

R

A

A

F

A

G

F

F

F

A

L

L

G

G

W

W

S

T

Y

Y

W

W

L

I

S

S

W

C

S

M

V

L

A

A

V

L

I

P

G

A

D

-

A

Q

V

V

V

S

G

S

G
x
T

F

F

F

Y

Q

L

Y

S

W

Y

F

Y

P

N

H

N

V

V

P

N

A

I

W

S

M

K

P

G

A

V

V

T

G

A

M

G

L

F

L

I

T

T

L

L

F

F

A

S

-

A

-

F

-

S

-

I

-

V

L

V

N

N

V

L

L

L

T

D

V

V

R

S

L

I

F

M

G

G

E

E

V

I

E

V

F

Y

W

V

F

A

A

G

I

I

I

S

K

K

L

V

I

I

A

I

V

A

L

I

T

L

L

M

I

V

G

F

V

T

S

M

G

I

V

I

L

L

I

N

A

A

S

G

S

H

-

G

-

N

-

D

-

I

-

H

-

E

-

G

-

V

-

G

-

F

-

R

F

Y

V

W

S

D

P

S

T

S

G

K

V

S

T

V

A

R

S

N

F

L

T

T

H

Y

-

G

-

L

-

Y

-

R

-

P

-

T

-

F

-

D

L

L

L

A

D

D

P

A

Q

G

A

E

A

G

F

S

P

K

N

K

G

G

L

I

F

S

G

G

F

P

F

K

A

G

G

R

F

F

-

L

-

A

-

T

-

A

-

S

-

V

-

M

Q

L

M

I

A

S

I

T

F

F

S

A

F

F

A

S

G

G

T

V

E

E

L

M

I

T

G

F

T

L

A

A

A

S

A

G

E

E

T

A

R

I

A

N

P

P

E

R

K

K

T

T

L

I

P

P

K

S

A

A

I

T

N

K

S

R

I

T

P

F

L

S

R

I

I

V

I

L

L

I

F

S

Y

Y

V

V

L

F

A

L

L

I

-

F

-

S

-

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

A

E

C

A

I

I

A

K

V

G

T

T

-

G

-

M

S

D

W

W

Q

R

H

L

V

R

S

T

P

N

S

C

K

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

-

S

S

S

P

P

F

W

V

V

E

V

L

A

F

L

L

Q

V

N

A

F

G

G

F

L

P

C

A

T

A

F

A

A

G

S

I

A

V

F

N

N

F

A

V

I

V

L

L

I

T

F

S

F

A

T

A

A

S

T

S

A

A

G

N

I

S

S

G

S

V

L

F

F

S

S

A

C

S

S

R

R

M

T

L

L

F

Y

G

A

L

M

A

S

D

V

L

Q

G

R

D

K

A

A

P

P

G

P

I

V

F

F

R

E

R

I

L

C

S

S

K

K

S

R

S

G

V

V

P

P

F

Y

I

V

S

S

L

V

A

I

F

F

T

S

L

L

L

F

M

S

L

V

L

I

G

A

L

Y

V

I

L

A

L

-

F

-

V

V

V

D

P

Q

E

T

V

A

M

I

T

E

A

N

F

F

T

D

I

V

V

L

S

A

T

N

V

V

S

S

A

S

I

A

L

S

V

T

I

S

F

I

T

I

W

W

S

M

T

G

I

L

L

N

A

L

S

S

Y

F

I

L

A

R

Y

F

R

Y

-

Y

-

A

-

L

K

K

K

Q

R

R

P

K

D

D

L

I

H

I

A

S

K

R

S

N

L

D

Y

S

K

S

M

Y

P

P

T382 (≠ G119) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P60061 Arginine/agmatine antiporter from Escherichia coli (strain K12) (see 3 papers)
24% identity, 81% coverage: 23:397/465 of query aligns to 15:385/445 of P60061

query
sites
P60061

I

V

Q

T

L

L

M

M

A

V

M

S

G

G

G

N

A
x
I

I

M

G

G

T
x
S

G

G

L

V

F

F

M

L

G

L

S

P

G

A

K

N

I

-

A

-

L

L

S

A

G

S

T

T

S

G

I

G

I

I

L

A

I

I

Y

Y

M

G

I

-

G

-

L

-

F

-

V

-

Y

-

F

W

V

L

M

V

R

T

A

I

M

I

G

G

E

A

L

L

L

G

L

L

S

S

N

M

L

V

N

Y

F

A

K

K

-

M

S

S

F

F

A

L

D

D

F

P

A

S

-

P

-

G

G

G

A

S

Y

Y

L

A

G

Y

P

A

R

R

A

R

A

C

F

F

-

G

-

P

F

F

L

L

G

G

W

Y

S

Q

-

T

-

N

-

V

-

L

Y
|
Y

W

W

L

L

S
x
A

W
x
C

S

W

V

I

A

G

V
x
N

I

I

G

A

D
x
M

A

V

V

V

V

I

V

G

G

V

G

G

F

Y

F

L

Q

S

Y

Y

W

F

F

F

P

P

H

I

V

L

P

K

A

D

-

P

-

L

-

V

-

L

W

T

M

I

P

T

A

C

V

V

G

V

M

V

L

L

L

W

T

I

L

F

F

V

A

L

L

L

N

N

V

I

L

V

T

G

V

P

R

K

L

M

F

I

G

T

E

R

V

V

E

Q

F

-

W

-

F

-

A

A

I

V

I

A

K

T

L

V

I

L

A

A

V

L

L

I

T

P

L

I

I

V

G

G

V

I

S

A

-

V

-

F

-

G

-

W

-

F

-

W

-

F

-

R

-

G

G

E

V

T

L

Y

I

M

A

A

S

A

S

W

F

N

V

V

S

S

P

G

T

L

G

G

V

T

T

F

A

G

S

A

F

I

T

Q

H

S

L

T

L

L

D

N

P

-

Q

-

A

-

F

-

P

-

N

-

G

-

L

-

F

-

G

-

F

-

A

-

G

-

F

-

Q

-

M

V

A

T

I

L

F
x
W

S
|
S

F

F

A
x
I

G

G

T

V

E

E

L

S

I

A

G

S

T

V

A

A

A

G

E

V

T

V

R

K

A

N

P

P

E

K

K

R

T

N

L

V

P

P

K

I

A

A

I

T

N

I

S

G

I

G

P

V

L

L

R

I

I

A

L

V

F

C

Y

Y

V

V

L

L

A

S

L

T

A

T

C

A

I

I

I

M

A

G

V

M

T

I

S

P

W

N

Q

A

H

A

V

L

S

R

P

V

S

S

K

A

S

S

P

P

F

F

V

G

E

D

L

A

F

A

L

R

V

M

A

A

G

L

F

G

P

D

A

T

A

A

A

G

G

A

I

I

V

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

A

L

S

G

S

S

A

L

N

G

S

G

G
x
W

V

T

F

L

S

L

A

A

S

G

R

Q

M

T

L

A

F

K

G

A

L

A

A

A

D

D

L

D

G

G

D

L

A

F

P

P

G

P

I

I

F

F

R

A

R

R

L

V

S

N

K

K

S

A

S

G

V

T

P

P

F

V

I

A

S

G

L

L

A

I

F

I

T

V

T

G

L

I

L

L

M

M

L

T

L

I

G

-

L

F

V

Q

L

L

L

S

F

S

V

I

V

S

P

P

E

N

V

A

M

T

T

K

A

E

F

F

T

G

I

L

V

V

S

S

T

S

V

V

S
|
S

A

V

I

I

L

F

V

T

I

L

F

V

T

P

W

Y

-

L

-

Y

-

T

-

C

-

A

S

A

T

L

I

L

L

L

A

L

S

G

Y

H

I

G

A

H

Y

F

R

G

K

K

K

A

R

R

P

P

I23 (≠ A31) binding ; binding
S26 (≠ T34) binding
Y93 (= Y102) mutation to L: Greatly decreased Arg uptake into liposomes.
A96 (≠ S105) binding ; binding
C97 (≠ W106) binding
N101 (≠ V110) binding ; mutation to A: Vmax for Arg-Agm exchange 1% of wild-type, KM increases 3-fold.; mutation to D: Nearly wild-type Arg-Agm exchange.
M104 (≠ D113) binding ; mutation to A: 30% decreased affinity for Arg, 50% decreased affinity for Agm.
W202 (≠ F218) binding ; mutation to L: Halves Arg uptake into liposomes.
S203 (= S219) binding
I205 (≠ A221) binding ; binding ; mutation to A: About wild-type affinity for Arg and Agm.
W293 (≠ G309) binding ; mutation W->C,H,L: Loss of Arg-Agm exchange.; mutation W->F,Y: Less than 20% Arg-Agm exchange activity. Vmax 15% of wild-type rate.
S357 (= S374) binding ; mutation to A: 20% decreased affinity for Arg, 40% decrease affinity for Agm.

P60063 Arginine/agmatine antiporter from Escherichia coli O157:H7 (see 3 papers)
24% identity, 81% coverage: 23:397/465 of query aligns to 15:385/445 of P60063

query
sites
P60063

I

V

Q

T

L

L

M

M

A

V

M

S

G

G

G
x
N

A
x
I

I

M

G
|
G

T
x
S

G
|
G

L

V

F

F

M

L

G

L

S

P

G

A

K

N

I

-

A

-

L

L

S

A

G

S

T

T

S

G

I

G

I

I

L

A

I

I

Y

Y

M

G

I

-

G

-

L

-

F

-

V

-

Y

-

F

W

V

L

M

V

R

T

A

I

M

I

G

G

E

A

L

L

L

G

L

L

S

S

N

M

L

V

N

Y

F

A

K

K

-

M

S

S

F

F

A

L

D

D

F

P

A

S

-

P

-

G

G

G

A

S

Y
|
Y

L

A

G

Y

P

A

R

R

A

R

A

C

F

F

-

G

-

P

F

F

L

L

G

G

W
x
Y

S

Q

-

T

-

N

-

V

-

L

Y
|
Y

W

W

L

L

S
x
A

W
x
C

S

W

V

I

A

G

V
x
N

I

I

G

A

D

M

A

V

V

V

V

I

V

G

G

V

G

G

F

Y

F

L

Q

S

Y

Y

W

F

F

F

P

P

H

I

V

L

P

K

A

D

-

P

-

L

-

V

-

L

W

T

M

I

P

T

A

C

V

V

G

V

M

V

L

L

L

W

T

I

L

F

F

V

A

L

L

L

N

N

V

I

L

V

T

G

V

P

R

K

L

M

F

I

G

T

E

R

V

V

E

Q

F

-

W

-

F

-

A

A

I

V

I

A

K

T

L

V

I

L

A

A

V

L

L

I

T

P

L

I

I

V

G

G

V

I

S

A

-

V

-

F

-

G

-

W

-

F

-

W

-

F

-

R

-

G

G

E

V

T

L

Y

I

M

A

A

S

A

S

W

F

N

V

V

S

S

P

G

T

L

G

G

V

T

T

F

A

G

S

A

F

I

T

Q

H

S

L

T

L

L

D

N

P

-

Q

-

A

-

F

-

P

-

N

-

G

-

L

-

F

-

G

-

F

-

A

-

G

-

F

-

Q

-

M

V

A

T

I

L

F
x
W

S

S

F

F

A
x
I

G
|
G

T
x
V

E
|
E

L
x
S

I
x
A

G

S

T

V

A

A

A

G

E

V

T

V

R

K

A

N

P

P

E

K

K

R

T

N

L

V

P

P

K

I

A

A

I

T

N

I

S

G

I

G

P

V

L

L

R

I

I

A

L

V

F

C

Y

Y

V

V

L

L

A

S

L

T

A

T

C

A

I

I

I

M

A

G

V

M

T

I

S

P

W

N

Q

A

H

A

V

L

S

R

P

V

S

S

K

A

S

S

P

P

F

F

V

G

E

D

L

A

F

A

L

R

V

M

A

A

G

L

F

G

P

D

A

T

A

A

A

G

G

A

I

I

V

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

A

L

S

G

S

S

A

L

N

G

S

G

G
x
W

V

T

F

L

S

L

A

A

S

G

R

Q

M

T

L

A

F

K

G

A

L

A

A

A

D

D

L

D

G

G

D

L

A

F

P

P

G

P

I

I

F

F

R

A

R

R

L

V

S

N

K

K

S

A

S

G

V

T

P

P

F

V

I

A

S

G

L

L

A

I

F

I

T

V

T

G

L

I

L

L

M

M

L

T

L

I

G

-

L
x
F

V

Q

L

L

L

S

F

S

V

I

V

S

P

P

E

N

V

A

M

T

T

K

A

E

F

F

T

G

I

L

V

V

S

S

T

S

V

V

S
|
S

A

V

I

I

L

F

V

T

I

L

F

V

T

P

W
x
Y

-

L

-

Y

-

T

-

C

-

A

S

A

T

L

I

L

L

L

A

L

S

G

Y

H

I

G

A

H

Y

F

R

G

K

K

K

A

R

R

P

P

N22 (≠ G30) mutation to A: No change in antiport activity, 6-fold higher affinity for Arg.
I23 (≠ A31) binding
GSG 25:27 (≠ GTG 33:35) Helix-breaking GSG motif TM1
S26 (≠ T34) binding ; mutation to K: 5% Agm antiport.
G27 (= G35) binding
Y74 (= Y89) mutation to A: 50% antiport activity at pH 6.0, 10-fold higher than wild-type antiport activity at pH 7.5, i.e. loss of pH-dependence of substrate transport. No change in binding of Arg or Agm.; mutation Y->C,H,L,M,Q,S: Loss of pH-dependence of substrate transport.; mutation to F: Approximately wild-type antiport.
Y87 (≠ W100) mutation to A: Markedly reduced binding affinity for Agm but not for Arg. 50% Agm antiport.
Y93 (= Y102) mutation to A: Reduced binding affinity for Arg, no binding to Agm. 25% Agm antiport.; mutation to K: Almost no binding to both Arg and Agm. 5% Agm antiport.
A96 (≠ S105) binding
C97 (≠ W106) binding
N101 (≠ V110) binding
W202 (≠ F218) Periplasmic (proximal) gate; binding
I205 (≠ A221) binding
GVESA 206:210 (≠ GTELI 222:226) Helix-breaking GVESA motif TM6
E208 (= E224) mutation E->A,D: 5-10% Agm antiport.
W293 (≠ G309) binding
F337 (≠ L354) mutation to A: Severely decreased antiport.
S357 (= S374) binding
Y365 (≠ W382) mutation to A: Markedly weakened binding to Arg but not to Agm. 5% Agm antiport.

3l1lA Structure of arg-bound escherichia coli adic (see paper)
23% identity, 81% coverage: 23:397/465 of query aligns to 9:368/423 of 3l1lA

query
sites
3l1lA

I

V

Q

T

L

L

M

M

A

V

M

S

G

G

G
x
A

A
x
I

I
x
M

G

G

T
x
S

G
|
G

L

V

F

F

M

L

G

L

S

P

G

A

K

N

I

-