SitesBLAST
Comparing GFF2918 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8rwgp 50S ribosomal protein L32
57% identity, 87% coverage: 10:86/89 of query aligns to 3:79/80 of 8rwgp
- binding : R3 (= R10), M14 (≠ R21), D15 (≠ A22), K16 (= K23), R24 (= R31), I30 (≠ L37), K33 (= K40), Y34 (≠ I41), K36 (≠ A43), R37 (≠ K44), S38 (= S45), T39 (≠ S46), L41 (≠ Y48), H42 (= H49), E60 (= E67), R62 (= R69), P63 (= P70), L64 (= L71), A65 (≠ S72), K66 (= K73), T67 (= T74), K68 (= K75), A69 (≠ N76), W70 (= W77)
3j9zSQ 30S ribosomal protein S13 (see paper)
54% identity, 89% coverage: 10:88/89 of query aligns to 5:83/83 of 3j9zSQ
- binding : S13 (= S18), K15 (= K20), M16 (≠ R21), E17 (≠ A22), S19 (≠ T24), R26 (= R31), I32 (≠ L37), K35 (= K40), K38 (≠ A43), R39 (≠ K44), T40 (≠ S45), T41 (≠ S46), K42 (= K47), H44 (= H49), R64 (= R69), P65 (= P70), S67 (= S72), K68 (= K73), K70 (= K75)
Sites not aligning to the query:
5no2Q Rsga-gdpnp bound to the 30s ribosomal subunit (rsga assembly intermediate) (see paper)
54% identity, 88% coverage: 10:87/89 of query aligns to 3:80/80 of 5no2Q
- binding : Y31 (= Y38), K33 (= K40)
- binding : R3 (= R10), S11 (= S18), M14 (≠ R21), K16 (= K23), R24 (= R31), Y31 (= Y38), F34 (≠ I41), K36 (≠ A43), R37 (≠ K44), T38 (≠ S45), T39 (≠ S46), L41 (≠ Y48), H42 (= H49), E60 (= E67), R62 (= R69), P63 (= P70), L64 (= L71), S65 (= S72), K66 (= K73), T67 (= T74), S69 (≠ N76), W70 (= W77)
8ayeQ 30S ribosomal protein S17 (see paper)
55% identity, 87% coverage: 10:86/89 of query aligns to 3:79/79 of 8ayeQ
- binding magnesium ion: S65 (= S72), K66 (= K73)
- binding : R3 (= R10), S11 (= S18), K13 (= K20), M14 (≠ R21), E15 (≠ A22), S17 (≠ T24), R24 (= R31), I30 (≠ L37), K33 (= K40), F34 (≠ I41), R37 (≠ K44), T38 (≠ S45), T39 (≠ S46), K40 (= K47), L41 (≠ Y48), H42 (= H49), E60 (= E67), R62 (= R69), P63 (= P70), L64 (= L71), S65 (= S72), K66 (= K73), T67 (= T74), K68 (= K75), S69 (≠ N76), W70 (= W77)
6v39q 30S ribosomal protein S18 (see paper)
57% identity, 87% coverage: 10:86/89 of query aligns to 4:80/80 of 6v39q
- binding : R4 (= R10), S12 (= S18), K14 (= K20), M15 (≠ R21), D16 (≠ A22), L31 (= L37), R37 (≠ A43), R38 (≠ K44), S39 (= S45), K41 (= K47), E61 (= E67), R63 (= R69), P64 (= P70), S66 (= S72), K67 (= K73), T68 (= T74), K69 (= K75)
5a9zBU of Thermous thermophilus ribosome bound to BipA-GDPCP (see paper)
55% identity, 90% coverage: 9:88/89 of query aligns to 2:81/104 of 5a9zBU
- binding : K2 (= K9), K13 (= K20), M14 (≠ R21), Q15 (≠ A22), T17 (= T24), R24 (= R31), L30 (= L37), Y31 (= Y38), K33 (= K40), R37 (≠ K44), S38 (= S45), K39 (≠ S46), K40 (= K47), Y41 (= Y48), E60 (= E67), R62 (= R69), P63 (= P70), I64 (≠ L71), S65 (= S72), K66 (= K73), R67 (≠ T74), K68 (= K75), R69 (≠ N76), F70 (≠ W77), R71 (≠ V78)
Sites not aligning to the query:
1i94Q Crystal structures of the small ribosomal subunit with tetracycline, edeine and if3 (see paper)
55% identity, 90% coverage: 9:88/89 of query aligns to 2:81/104 of 1i94Q
- binding magnesium ion: R24 (= R31), K36 (≠ A43), R37 (≠ K44), S65 (= S72), K66 (= K73), R67 (≠ T74), K68 (= K75)
- binding : K13 (= K20), Q15 (≠ A22), L30 (= L37), R37 (≠ K44), S38 (= S45), R62 (= R69), P63 (= P70), I64 (≠ L71), S65 (= S72), K66 (= K73)
Sites not aligning to the query:
8crxQ 30S ribosomal protein S17 (see paper)
49% identity, 96% coverage: 2:86/89 of query aligns to 5:89/90 of 8crxQ
- binding : R8 (≠ K5), T10 (≠ S7), R11 (≠ L8), R12 (≠ K9), K13 (≠ R10), S21 (= S18), K23 (= K20), M24 (≠ R21), N25 (≠ A22), K26 (= K23), T27 (= T24), K43 (= K40), R50 (≠ K47), K52 (≠ H49), M69 (≠ T66), E70 (= E67), T71 (≠ S68), R72 (= R69), P73 (= P70), S75 (= S72), A76 (≠ K73), T77 (= T74), K78 (= K75), R79 (≠ N76), R81 (≠ V78)
Sites not aligning to the query:
8rd8QZ Small ribosomal subunit protein uS5 (see paper)
50% identity, 83% coverage: 12:85/89 of query aligns to 4:77/79 of 8rd8QZ
- binding : S10 (= S18), M13 (≠ R21), D14 (≠ A22), K15 (= K23), S16 (≠ T24), L29 (= L37), K32 (= K40), Q33 (≠ I41), R35 (≠ A43), R36 (≠ K44), S37 (= S45), T38 (≠ S46), K39 (= K47), K41 (≠ H49), E59 (= E67), R61 (= R69), P62 (= P70), I63 (≠ L71), S64 (= S72), K65 (= K73), T66 (= T74), K67 (= K75), W69 (= W77)
7bgdq Staphylococcus aureus 30s ribosomal subunit in presence of spermidine (body only)
47% identity, 91% coverage: 9:89/89 of query aligns to 6:86/86 of 7bgdq
- binding : R6 (≠ K9), K7 (≠ R10), Y9 (≠ L12), K17 (= K20), M18 (≠ R21), D19 (≠ A22), K20 (= K23), T21 (= T24), Y29 (≠ R32), K30 (≠ V33), T31 (≠ K34), Y35 (= Y38), K37 (= K40), K40 (≠ A43), Y41 (≠ K44), S42 (= S45), K43 (≠ S46), K44 (= K47), Y45 (= Y48), K46 (≠ H49), Q63 (≠ T66), E64 (= E67), T65 (≠ S68), R66 (= R69), P67 (= P70), L68 (= L71), S69 (= S72), A70 (≠ K73), K72 (= K75), R73 (≠ N76), R75 (≠ V78)
Sites not aligning to the query:
3j9wAQ 30S ribosomal protein uS9 (see paper)
49% identity, 85% coverage: 14:89/89 of query aligns to 11:86/86 of 3j9wAQ
5myjAQ of 70S ribosome from Lactococcus lactis (see paper)
46% identity, 85% coverage: 14:89/89 of query aligns to 7:82/82 of 5myjAQ
- binding : S11 (= S18), K13 (= K20), M14 (≠ R21), D15 (≠ A22), K16 (= K23), R26 (≠ V33), V30 (≠ L37), R34 (≠ I41), K39 (≠ S46), Y41 (= Y48), K42 (≠ H49), E46 (= E53), M59 (≠ T66), E60 (= E67), T61 (≠ S68), R62 (= R69), P63 (= P70), L64 (= L71), S65 (= S72), K66 (= K73), D67 (≠ T74), K68 (= K75), H69 (≠ N76), R71 (≠ V78), V73 (≠ T80)
Sites not aligning to the query:
A0QSE0 Small ribosomal subunit protein uS17; 30S ribosomal protein S17 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 88% coverage: 9:86/89 of query aligns to 20:97/98 of A0QSE0
- K96 (= K85) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
6dzi5 50S ribosomal protein L29 (see paper)
45% identity, 88% coverage: 9:86/89 of query aligns to 17:94/94 of 6dzi5
- binding : R17 (≠ K9), K28 (= K20), M29 (≠ R21), Q30 (≠ A22), R40 (= R32), K41 (≠ V33), Y46 (= Y38), T53 (≠ S45), K55 (= K47), K57 (≠ H49), M74 (≠ T66), T76 (≠ S68), R77 (= R69), P78 (= P70), L79 (= L71), S80 (= S72), A81 (≠ K73), T82 (= T74), K83 (= K75), R84 (≠ N76), R86 (≠ V78)
Sites not aligning to the query:
7mscq 70SIC in complex with MtbEttA at Pre_R0 state (see paper)
45% identity, 88% coverage: 9:86/89 of query aligns to 17:94/95 of 7mscq
- binding : R17 (≠ K9), S26 (= S18), K28 (= K20), M29 (≠ R21), Q30 (≠ A22), T32 (= T24), M41 (≠ V33), Y46 (= Y38), K48 (= K40), K54 (≠ S46), K55 (= K47), K57 (≠ H49), M74 (≠ T66), R77 (= R69), P78 (= P70), S80 (= S72), A81 (≠ K73), T82 (= T74), K83 (= K75), R84 (≠ N76), R86 (≠ V78)
Sites not aligning to the query:
6xywBp At4g35490 (see paper)
39% identity, 87% coverage: 12:88/89 of query aligns to 1:77/78 of 6xywBp
- binding : S7 (= S18), M10 (≠ R21), Q11 (≠ A22), S13 (≠ T24), R20 (= R31), F22 (≠ V33), I26 (≠ L37), K32 (≠ A43), R33 (≠ K44), T34 (≠ S45), S35 (= S46), R58 (= R69), P59 (= P70), S61 (= S72), K62 (= K73), H65 (≠ N76)
6o8wq 30S ribosomal protein S10 (see paper)
48% identity, 82% coverage: 14:86/89 of query aligns to 10:82/83 of 6o8wq
- binding : K16 (= K20), M17 (≠ R21), D18 (≠ A22), K19 (= K23), T20 (= T24), Y34 (= Y38), K36 (= K40), R37 (≠ I41), K39 (≠ A43), Y40 (≠ K44), K42 (≠ S46), K43 (= K47), K45 (≠ H49), M62 (≠ T66), T64 (≠ S68), R65 (= R69), P66 (= P70), L67 (= L71), S68 (= S72), A69 (≠ K73), K71 (= K75), R72 (≠ N76)
Sites not aligning to the query:
8uu6q Large ribosomal subunit protein uL24 (see paper)
52% identity, 78% coverage: 9:77/89 of query aligns to 4:72/80 of 8uu6q
- binding : R4 (≠ K9), K15 (= K20), D17 (≠ A22), R36 (≠ I41), K38 (≠ A43), S40 (= S45), K42 (= K47), K44 (≠ H49), R64 (= R69), P65 (= P70), S67 (= S72), T69 (= T74), K70 (= K75), H71 (≠ N76)
Sites not aligning to the query:
8uu7q Small ribosomal subunit protein uS17 (see paper)
52% identity, 78% coverage: 9:77/89 of query aligns to 5:73/81 of 8uu7q
- binding : R5 (≠ K9), M17 (≠ R21), D18 (≠ A22), K19 (= K23), K30 (= K34), L33 (= L37), R37 (≠ I41), S41 (= S45), K42 (≠ S46), K43 (= K47), E63 (= E67), T64 (≠ S68), R65 (= R69), P66 (= P70), S68 (= S72), A69 (≠ K73), T70 (= T74), H72 (≠ N76)
Sites not aligning to the query:
8p7yP 50S ribosomal protein L31 (see paper)
45% identity, 92% coverage: 5:86/89 of query aligns to 2:84/85 of 8p7yP
- binding magnesium ion: K20 (= K23), H48 (= H51)
- binding : R3 (≠ T6), R6 (≠ K9), N18 (≠ R21), K20 (= K23), V40 (≠ A43), R41 (≠ K44), H42 (≠ S45), K43 (≠ S46), K44 (= K47), Y45 (= Y48), Q46 (≠ H49), T66 (≠ S68), R67 (= R69), P68 (= P70), L69 (= L71), S70 (= S72), A71 (≠ K73), R74 (≠ N76), R76 (≠ V78)
Query Sequence
>GFF2918
MTEAKTSLKRTLVGKVVSDKRAKTVTVLVERRVKHELYDKIVAKSSKYHAHDEKSEYKMG
DVIEITESRPLSKTKNWVATRLVQKAALV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory