SitesBLAST

Q9CPU0 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Mus musculus (Mouse) (see paper)
61% identity, 92% coverage: 8:175/182 of query aligns to 8:180/184 of Q9CPU0

query
sites
Q9CPU0

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Q34 (= Q26) binding
E100 (= E95) binding
H127 (= H122) binding in other chain
E173 (= E168) binding in other chain

4kykA Crystal structure of mouse glyoxalase i complexed with indomethacin (see paper)
61% identity, 92% coverage: 8:175/182 of query aligns to 3:175/179 of 4kykA

query
sites
4kykA

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active site: Q29 (= Q26), E95 (= E95), H122 (= H122), E168 (= E168)
binding zinc ion: Q29 (= Q26), E95 (= E95), H122 (= H122), E168 (= E168)

4kyhA Crystal structure of mouse glyoxalase i complexed with zopolrestat (see paper)
61% identity, 92% coverage: 8:175/182 of query aligns to 3:175/177 of 4kyhA

query
sites
4kyhA

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active site: Q29 (= Q26), E95 (= E95), H122 (= H122), E168 (= E168)
binding zinc ion: Q29 (= Q26), E95 (= E95), H122 (= H122), E168 (= E168)
binding 3,4-dihydro-4-oxo-3-((5-trifluoromethyl-2-benzothiazolyl)methyl)-1-phthalazine acetic acid: M31 (= M28), R33 (= R30), F63 (= F60), E95 (= E95), T97 (= T97), N99 (= N99)

2za0A Crystal structure of mouse glyoxalase i complexed with methyl-gerfelin (see paper)
61% identity, 92% coverage: 8:175/182 of query aligns to 5:177/180 of 2za0A

query
sites
2za0A

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active site: Q31 (= Q26), E97 (= E95), H124 (= H122), E170 (= E168)
binding methyl 4-(2,3-dihydroxy-5-methylphenoxy)-2-hydroxy-6-methylbenzoate: Q31 (= Q26), F65 (= F60), L67 (= L62), F90 (= F88), E97 (= E95), H124 (= H122), M155 (= M153), E170 (= E168)
binding zinc ion: Q31 (= Q26), E97 (= E95), H124 (= H122), E170 (= E168)

4pv5A Crystal structure of mouse glyoxalase i in complexed with 18-beta- glycyrrhetinic acid (see paper)
62% identity, 93% coverage: 6:175/182 of query aligns to 2:168/170 of 4pv5A

query
sites
4pv5A

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active site: Q22 (= Q26), E88 (= E95), H115 (= H122), E161 (= E168)
binding (3beta,5beta,14beta)-3-hydroxy-11-oxoolean-12-en-29-oic acid: R111 (≠ Q118), K139 (= K146)
binding zinc ion: Q22 (= Q26), E88 (= E95)

4opnA Crystal structure of mouse glyoxalase i complexed with mah
61% identity, 91% coverage: 10:175/182 of query aligns to 2:172/172 of 4opnA

query
sites
4opnA

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x
I

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding L-gamma-glutamyl-N-(3-ethynylphenyl)-N-hydroxy-L-glutaminylglycine: Q26 (= Q26), R30 (= R30), F60 (= F60), F85 (= F88), E92 (= E95), T94 (= T97), N96 (= N99), R115 (≠ Q118), H119 (= H122), K143 (= K146), K149 (= K152), M150 (= M153), L153 (= L156), E165 (= E168), I172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95)

4x2aA Crystal structure of mouse glyoxalase i complexed with baicalein (see paper)
65% identity, 87% coverage: 17:175/182 of query aligns to 11:166/167 of 4x2aA

query
sites
4x2aA

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S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K

K

P

P

D

D

G

G

K

K

M
|
M

K

K

G

G

L

L

A

A

F

F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
I

active site: Q20 (= Q26), E86 (= E95), H113 (= H122), E159 (= E168)
binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: Q20 (= Q26), F49 (= F55), L56 (= L62), F79 (= F88), E86 (= E95), H113 (= H122), M144 (= M153), E159 (= E168), I166 (≠ L175)
binding zinc ion: Q20 (= Q26), E86 (= E95), H113 (= H122), E159 (= E168)

Sites not aligning to the query:

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: 167

3w0uA Human glyoxalase i with an n-hydroxypyridone inhibitor
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/175 of 3w0uA

query
sites
3w0uA

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R

R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R

Q

K

K

L

C

D

D

F

F

P

P

E

I

M

M

K

K

F
|
F

T

S

L
|
L

Y

Y

F

F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T

I

T

A

Y

W

T

A

F

L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T

T

H

H

N

N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q

R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K
|
K

K

K

P

P

D

D

G

G

K

K

M
|
M

K

K

G

G

L

L

A

A

F

F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: Q26 (= Q26), F60 (= F60), L62 (= L62), E92 (= E95), H119 (= H122), K143 (= K146), M150 (= M153), E165 (= E168), M172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

3w0tA Human glyoxalase i with an n-hydroxypyridone derivative inhibitor
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/176 of 3w0tA

query
sites
3w0tA

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R

R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R

Q

K

K

L

C

D

D

F
|
F

P

P

E

I

M

M

K

K

F
|
F

T

S

L
|
L

Y

Y

F
|
F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T

I

T

A

Y

W

T

A

F

L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T

T

H

H

N

N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q

R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K

K

P

P

D

D

G

G

K

K

M

M

K

K

G

G

L

L

A

A

F

F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: Q26 (= Q26), F55 (= F55), F60 (= F60), L62 (= L62), F64 (= F64), E92 (= E95), H119 (= H122), E165 (= E168), M172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

Sites not aligning to the query:

binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: 176

3vw9A Human glyoxalase i with an n-hydroxypyridone inhibitor (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/176 of 3vw9A

query
sites
3vw9A

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R

R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R

Q

K

K

L
x
C

D

D

F

F

P

P

E

I

M

M

K

K

F

F

T

S

L
|
L

Y

Y

F

F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T

I

T

A

Y

W

T

A

F

L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T

T

H

H

N

N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q

R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K

K

P

P

D

D

G

G

K

K

M
|
M

K

K

G

G

L

L

A

A

F
|
F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: Q26 (= Q26), C53 (≠ L53), L62 (= L62), E92 (= E95), H119 (= H122), M150 (= M153), F155 (= F158), E165 (= E168), M172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

Sites not aligning to the query:

binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: 176

1qipA Human glyoxalase i complexed with s-p- nitrobenzyloxycarbonylglutathione (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/176 of 1qipA

query
sites
1qipA

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R
|
R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R
x
Q

K

K

L
x
C

D

D

F

F

P

P

E

I

M

M

K

K

F
|
F

T

S

L

L

Y

Y

F
|
F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T
x
I

T

A

Y

W

T

A

F
x
L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T
|
T

H

H

N
|
N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q
x
R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K

K

P

P

D

D

G

G

K

K

M
|
M

K

K

G

G

L

L

A

A

F
|
F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding s-p-nitrobenzyloxycarbonylglutathione: R30 (= R30), Q51 (≠ R51), C53 (≠ L53), F60 (= F60), F64 (= F64), I81 (≠ T84), L85 (≠ F88), T94 (= T97), N96 (= N99), R115 (≠ Q118), M150 (= M153), F155 (= F158), M172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

1qinA Human glyoxalase i complexed with s-(n-hydroxy-n-p- iodophenylcarbamoyl) glutathione (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/176 of 1qinA

query
sites
1qinA

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R
|
R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R

Q

K

K

L

C

D

D

F

F

P

P

E

I

M

M

K

K

F
|
F

T

S

L
|
L

Y

Y

F
|
F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T

I

T

A

Y

W

T

A

F

L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T
|
T

H

H

N
|
N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q
x
R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K
|
K

K

K

P

P

D

D

G
|
G

K
|
K

M
|
M

K

K

G

G

L

L

A

A

F

F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L
x
M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: Q26 (= Q26), R30 (= R30), F60 (= F60), L62 (= L62), F64 (= F64), E92 (= E95), T94 (= T97), N96 (= N99), R115 (≠ Q118), H119 (= H122), K143 (= K146), G148 (= G151), K149 (= K152), M150 (= M153), E165 (= E168), M172 (≠ L175)
binding zinc ion: Q26 (= Q26), E92 (= E95)

1froA Human glyoxalase i with benzyl-glutathione inhibitor (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/176 of 1froA

query
sites
1froA

E

E

Q

A

A

A

P

L

G

S

L

C

Y

C

E

S

E

D

T

A

V

D

P

P

E

S

T

T

E

K

G

D

Y

F

V

L

F

L

N

Q

Q
|
Q

T

T

M

M

M

L

R
|
R

I

V

K

K

E

D

P

P

E

K

R

K

S

S

M

L

D

D

F

F

Y

Y

T

T

R

R

V

V

M

L

G

G

M

M

R

T

L

L

V

I

R

Q

K

K

L

C

D

D

F

F

P

P

E

I

M

M

K

K

F
|
F

T

S

L

L

Y

Y

F

F

L

L

G

A

Y

Y

L

E

D

D

D

K

R

N

Q

D

A

-

G

-

L

-

V

I

P

P

Q

K

D

E

D

K

A

D

H

E

R

K

T

I

T

A

Y

W

T

A

F

L

G

S

R

R

E

K

A

A

M

T

L

L

E
|
E

L

L

T
|
T

H

H

N
|
N

W

W

G

G

T

T

E

E

D

D

N

E

D

T

F

Q

G

S

Y

Y

H

H

N

N

G

G

N

N

D

S

E

D

P

P

Q
x
R

G

G

F

F

G

G

H
|
H

I

I

G

G

V

I

A

A

V

V

P

P

D

D

V

V

Y

Y

A

S

A

A

C

C

D

K

R

R

F

F

Q

E

K

E

L

L

G

G

V

V

E

K

F

F

V

V

K

K

P

P

D

D

G

G

K

K

M
|
M

K

K

G

G

L

L

A

A

F
|
F

I

I

K

Q

D

D

P

P

D

D

G

G

Y

Y

W

W

I

I

E
|
E

I

I

L

L

Q

N

P

P

D

N

M

K

L

M

active site: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)
binding s-benzyl-glutathione: R30 (= R30), F60 (= F60), T94 (= T97), N96 (= N99), R115 (≠ Q118), M150 (= M153), F155 (= F158), E165 (= E168)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

Sites not aligning to the query:

binding s-benzyl-glutathione: 176

Q04760 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Homo sapiens (Human) (see 12 papers)
59% identity, 93% coverage: 6:175/182 of query aligns to 14:180/184 of Q04760

query
sites
Q04760

E

E

Q

A

A

A

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L
x
C

Y
x
C

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M

C19 (≠ L11) modified: Disulfide link with 20; to Y: in dbSNP:rs17855424; mutation to A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-20. Loss of NO-mediated modification; when associated with A-139.
C20 (≠ Y12) modified: Disulfide link with 19; mutation to A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-19. Loss of NO-mediated modification; when associated with A-139.
Q34 (= Q26) binding ; mutation to E: Reduces enzyme activity by 99%.
S45 (= S37) mutation to A: No effect on phosphorylation.
C61 (≠ L53) modified: Disulfide link with 139, Alternate; mutation to A: No effect on NO-mediated modification.
S69 (≠ T61) mutation to A: No effect on phosphorylation.
S94 (≠ G89) mutation to A: No effect on phosphorylation.
T98 (≠ M93) mutation to A: No effect on phosphorylation.
E100 (= E95) binding ; mutation to Q: Reduces enzyme activity by over 99%.
T102 (= T97) mutation to A: No effect on phosphorylation.
T107 (= T102) modified: Phosphothreonine; mutation to A: Loss of phosphorylation.
E111 (≠ N106) to A: in dbSNP:rs4746
H127 (= H122) binding in other chain
C139 (= C134) modified: S-glutathionyl cysteine; alternate; modified: Disulfide link with 61, Alternate; mutation to A: Impaired NO-mediated modification. Loss of NO-mediated modification; when associated with A-19 or A-20.
E173 (= E168) active site, Proton donor/acceptor; binding in other chain; mutation to Q: Abolishes enzyme activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

7wt0A Human glyoxalase i (with c-ter his tag) in complex with tlsc702 (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 13:179/185 of 7wt0A

query
sites
7wt0A

E

E

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x
M

binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: C60 (≠ L53), F62 (= F55), L69 (= L62), F71 (= F64), L92 (≠ F88), E99 (= E95), H126 (= H122), K150 (= K146), M157 (= M153), L160 (= L156), F162 (= F158), E172 (= E168), M179 (≠ L175)
binding zinc ion: Q33 (= Q26), E99 (= E95), H126 (= H122), E172 (= E168)

Sites not aligning to the query:

binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: 183

7wszA Human glyoxalase i (with c-ter his tag) in glycerol-bound form (see paper)
59% identity, 93% coverage: 6:175/182 of query aligns to 6:172/183 of 7wszA

query
sites
7wszA

E

E

Q

A

A

A

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C

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C

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A

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binding glycerol: I50 (≠ V50), Q51 (≠ R51), K52 (= K52), K80 (≠ R83), H119 (= H122), M150 (= M153), E165 (= E168)
binding zinc ion: Q26 (= Q26), E92 (= E95), H119 (= H122), E165 (= E168)

Sites not aligning to the query:

binding glycerol: 176

1bh5A Human glyoxalase i q33e, e172q double mutant (see paper)
58% identity, 93% coverage: 6:175/182 of query aligns to 7:173/177 of 1bh5A

query
sites
1bh5A

E

E

Q

A

A

A

P

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C

Y

C

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L

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active site: E27 (≠ Q26), E93 (= E95), H120 (= H122), Q166 (≠ E168)
binding s-hexylglutathione: R31 (= R30), F61 (= F60), L63 (= L62), T95 (= T97), N97 (= N99), R116 (≠ Q118), M151 (= M153), F156 (= F158)
binding zinc ion: E27 (≠ Q26), E93 (= E95), H120 (= H122), Q166 (≠ E168)

P50107 Glyoxalase I; Glx I; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; actoylglutathione lyase; EC 4.4.1.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
49% identity, 82% coverage: 21:170/182 of query aligns to 180:320/326 of P50107

query
sites
P50107

G

G

Y

N

V

K

F

F

N

N

Q

H

T

T

M

M

M

I

R

R

I

I

K

K

E

N

P

P

E

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R

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F

F

Y

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V

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M

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A

K

K

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F

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L

Y

Y

F

F

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L

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G

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Y

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G

D

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R

-

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-

A

-

G

-

L

-

V

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P

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D

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F

F

G

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R

C

E

E

A

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L

L

E

E

L

L

T

T

H

H

N

N

W

W

G

G

T

T

E

E

D

N

D

D

N

P

D

N

F

F

G

H

Y

Y

H

H

N

N

G

G

N

N

D

S

E

E

P

P

Q

Q

G

G

F

Y

G

G

H

H

I

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D

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A

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C

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G

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D

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E

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E318 (= E168) active site, Proton donor/acceptor

Sites not aligning to the query:

163 active site, Proton donor/acceptor

Query Sequence

>GFF2946 FitnessBrowser__Marino:GFF2946
MPKHFEQAPGLYEETVPETEGYVFNQTMMRIKEPERSMDFYTRVMGMRLVRKLDFPEMKF
TLYFLGYLDDRQAGLVPQDDAHRTTYTFGREAMLELTHNWGTEDDNDFGYHNGNDEPQGF
GHIGVAVPDVYAACDRFQKLGVEFVKKPDDGKMKGLAFIKDPDGYWIEILQPDMLEKQRK
ED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory