SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3010 FitnessBrowser__WCS417:GFF3010 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4jbbA Crystal structure of glutathione s-transferase a6tby7(target efi- 507184) from klebsiella pneumoniae mgh 78578, gsh complex
53% identity, 97% coverage: 1:198/205 of query aligns to 4:204/208 of 4jbbA

query
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4jbbA

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active site: Y51 (≠ L48), A52 (≠ S49)
binding glutathione: S14 (= S11), Y16 (= Y13), H43 (≠ Q40), R56 (= R53), V57 (= V54), E69 (= E66), S70 (= S67)

P77544 Glutathione S-transferase YfcF; EC 2.5.1.18 from Escherichia coli (strain K12) (see paper)
53% identity, 97% coverage: 1:198/205 of query aligns to 6:206/214 of P77544

query
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P77544

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S10 (≠ V5) mutation to A: Nearly no effect on GSH transferase activity.
S16 (= S11) mutation to G: 2% of wild-type GSH transferase activity.

3bbyA Crystal structure of glutathione s-transferase (np_416804.1) from escherichia coli k12 at 1.85 a resolution
51% identity, 97% coverage: 1:198/205 of query aligns to 4:186/191 of 3bbyA

query
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3bbyA

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binding calcium ion: D75 (= D87), E77 (≠ K89)

4isdA Crystal structure of glutathione transferase homolog from burkholderia gl bgr1, target efi-501803, with bound glutathione
48% identity, 99% coverage: 3:204/205 of query aligns to 7:187/187 of 4isdA

query
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4isdA

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binding glutathione: Q43 (= Q39), R51 (= R53), V52 (= V54), E64 (= E66), S65 (= S67)

4hojA Crystal structure of glutathione transferase homolog from neisseria gonorrhoeae, target efi-501841, with bound glutathione
30% identity, 95% coverage: 10:204/205 of query aligns to 8:191/197 of 4hojA

query
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binding calcium ion: D158 (vs. gap), D161 (vs. gap), L172 (≠ V182), E176 (≠ Q186)
binding glutathione: C9 (≠ S11), F11 (≠ Y13), K36 (≠ H41), Q48 (≠ R53), V49 (= V54), E61 (= E66), S62 (= S67)

4qq7A Crystal structure of putative stringent starvation protein a from burkholderia cenocepacia with bound glutathione
30% identity, 90% coverage: 10:193/205 of query aligns to 10:190/204 of 4qq7A

query
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binding D-glucose: F94 (≠ V97), F98 (≠ L101), I138 (≠ L144), M145 (≠ Y151)
binding glutathione: C11 (≠ S11), F13 (≠ Y13), K38 (≠ A38), Q50 (≠ R53), V51 (= V54), E63 (= E66), S64 (= S67), E110 (≠ V116), N111 (≠ V117)

3qawA Crystal structure of a glutathione-s-transferase from antarctic clam laternula elliptica in a complex with glutathione (see paper)
33% identity, 42% coverage: 11:96/205 of query aligns to 11:96/219 of 3qawA

query
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3qawA

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|
S

P

P

Y
x
P

A

C

L

W

S

K

V

V

F

L

V

L

T

V

L

L

R

Q

E

E

K

K

G

K

L

I

A

D

F

Y

D

D

T

E

I

K

T

I

L

I

D

S

L
x
F

D

S

A

K

Q

E

Q
x
H

H
x
K

A

S

A

E

D

E

F

I

A

L

R

E

L

L

S

N

L

P

T

R

Q

G

R
x
Q

V
|
V

P

P

T

T

L

F

V

T

E

D

G

G

D

D

F

V

A

V

L

V

S

N

E
|
E

S
|
S

S

T

A

A

I

I

T

C

E

M

Y

Y

L

L

E

E

Q

E

A

K

Y

Y

P

P

G

K

T

V

P

P

V

L

Y

F

P

P

A

S

D

D

P

T

K

T

L

I

R

R

A

A

R

K

A

V

R

Y

Q

Q

active site: S11 (= S11)
binding glutathione: S11 (= S11), P13 (≠ Y13), F35 (≠ L35), H40 (≠ Q40), K41 (≠ H41), Q53 (≠ R53), V54 (= V54), E66 (= E66), S67 (= S67)

Q64471 Glutathione S-transferase theta-1; GST class-theta-1; EC 2.5.1.18 from Mus musculus (Mouse) (see paper)
29% identity, 79% coverage: 1:161/205 of query aligns to 3:168/240 of Q64471

query
sites
Q64471

M

L

R

E

L

L

Y

Y

V

L

D

D

H

L

M

L

Y

S

T

Q

S

P

P

C

Y

R

A

A

L

I

S

Y

V

I

F

F

V

A

T

-

L

-

R

K

E

K

K

N

G

N

L

I

A

P

F

F

D

Q

T

M

I

H

T

T

L

V

D

E

L

L

D

R

A

K

A

G

Q

E

Q

H

H

L

A

S

A

D

D

A

F

F

A

A

R

R

L

V

S

N

L

P

T

M

Q

K

R

R

V

V

P

P

T

A

L

M

V

M

E

D

G

G

D

G

F

F

A

T

L

L

S

C

E

E

S

S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

E

A

Q

H

A

K

Y

Y

P

-

G

K

T

V

P

P

V

D

-

H

-

W

Y

Y

P

P

A

Q

D

D

P

L

K

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

V

Y

Q

L

A

A

W

W

-

Q

-

H

-

T

-

G

L

L

R

R

S

R

D

S

L

C

L

L

P

R

I

A

R

L

Q

W

E

H

R

K

S

V

T

M

M

F

V

P

V

V

F

F

Y

L

G

G

Q

E

K

Q

M

I

P

P

L

E

S

T

P

L

V

A

A

A

E

T

A

L

A

A

A

E

A

L

K

D

L

V

-

N

I

L

S

Q

A

V

A

L

Q

E

D

D

L

K

L

F

V

L

G

Q

N

D

P

K

A

D

Y

F

L

L

F

V

G

G

-

P

D

H

W

I

S

S

I

L

A

A

D

D

V

L

Sites not aligning to the query:

234 R→W: Strongly reduced catalytic activity.

4ecjA Crystal structure of glutathione s-transferase prk13972 (target efi- 501853) from pseudomonas aeruginosa pacs2 complexed with glutathione
30% identity, 75% coverage: 8:161/205 of query aligns to 5:160/204 of 4ecjA

query
sites
4ecjA

M

L

Y

Y

T

T

-

A

S
x
T

P

P

Y
x
N

A

G

L

H

S

K

V

V

F

S

V

I

T

A

L

L

R

E

E

E

K

M

G

G

L

L

A

P

F

Y

D

R

T

V

I

H

T

A

L

L

D

S

L
x
F

D

D

A

K

Q

E

Q

Q

H

K

A

A

A

P

D

E

F

F

A

L

R

R

L

I

S

N

L

P

T

N

Q

G

R
|
R

V
x
I

P

P

T

A

L

I

V

V

E

D

-

R

-

D

-

N

G

D

D

D

F

F

A

A

L

V

S

F

E
|
E

S
|
S

S

G

A

A

I

I

T

L

E

I

Y

Y

L

L

E

-

Q

-

A

A

Y

E

P

K

G

T

T

G

P

Q

V

L

Y

M

P

P

A

A

D

D

P

V

K

K

L

G

R

R

A

S

R

R

A

V

R

I

Q

Q

V

W

Q

L

A

M

W

F

L

Q

R

M

S

G

D

G

L

V

L

G

P

P

I

M

R

Q

Q

G

E

Q

R

A

S

N

T

V

M

F

V

F

V

R

F

Y

Y

F

G

P

Q

E

K

K

M

L

P

Q

P

G

L

A

S

I

P

D

V

R

A

Y

E

Q

A

H

A

E

A

T

A

R

K

R

L

L

I

Y

S

E

A

V

A

L

Q

-

D

D

L

G

L

R

V

L

G

G

N

E

P

A

A

E

Y

Y

L

L

F

A

G

G

D

D

W

Y

S

S

I

I

A

A

D

D

V

I

active site: T10 (≠ S11)
binding glutathione: T10 (≠ S11), N12 (≠ Y13), F34 (≠ L35), R52 (= R53), I53 (≠ V54), E68 (= E66), S69 (= S67)

4kdxA Crystal structure of a glutathione transferase family member from burkholderia graminis, target efi-507264, bound gsh, ordered domains, space group p21, form(1)
31% identity, 83% coverage: 10:179/205 of query aligns to 9:184/207 of 4kdxA

query
sites
4kdxA

T

T

S
|
S

P

I

Y
x
N

A

V

L

R

S

K

V

V

F

L

V

W

T

T

L

C

R

A

E

E

K

L

G

G

L

L

A

A

F

F

D

E

T

R

I

E

T

D

L

W

D
x
G

L
x
A

D

G

A

F

A

R

Q

P

Q

T

H

N

A

V

A

P

D

E

F

F

A

L

R

A

L

L

S

N

L

P

T

N

Q

A

R
x
M

V
|
V

P

P

T

V

L

I

V

R

E

D

G

G

D

D

F

F

A

V

L

L

S

W

E
|
E

S
|
S

S

N

A

S

I

I

T

I

E

R

Y

Y

L

L

E

A

Q

G

A

R

Y

Y

P

G

G

G

T

E

P

W

V

L

Y

Y

P

P

A

A

D

D

P

A

K

R

L

E

R

R

A

A

R

R

A

C

R

D

Q

Q

V

W

Q

I

A

D

W

W

L

Q

R

A

S

S

D

E

L

L

L

-

P

-

I

-

R

-

Q

-

E

N

R

R

S

S

T

W

M

S

V

Y

V

A

F

F

Y

L

G

A

-

L

Q

V

K

R

M

Q

P

S

P

P

L

A

S

H

P

R

V

D

A

A

E

Q

-

Q

-

I

-

E

-

A

-

S

-

R

A

A

A

N

A

W

A

A

K

K

L

H

I

M

S

A

A

I

A

V

Q

E

D

G

L

Q

L

L

V

Q

G

R

N

T

P

G

A

A

Y

F

L

I

F

A

G

G

D

D

-

A

W

F

S

S

I

L

A

A

D

D

V

I

D

P

L

I

A

A

V

L

M

S

L

I

N

N

R

R

L

W

I

L

-

E

-

T

-

P

L

I

N

A

G

R

D

D

S

D

V

L

P

P

A

A

active site: S10 (= S11)
binding glutathione: S10 (= S11), N12 (≠ Y13), G33 (≠ D34), A34 (≠ L35), M52 (≠ R53), V53 (= V54), E65 (= E66), S66 (= S67)

P30711 Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1; EC 2.5.1.18 from Homo sapiens (Human) (see 2 papers)
30% identity, 79% coverage: 1:161/205 of query aligns to 3:168/240 of P30711

query
sites
P30711

M

L

R

E

L

L

Y

Y

V

L

D

D

H

-

M

L

Y

L

T

S

S

Q

P

P

Y

-

A

C

L

R

S

A

V

V

F

Y

V

I

T

F

L

A

R

K

E

K

K

N

G

D

L

I

A

P

F

F

D

E

T

L

I

R

T

I

L

V

D

D

L

L

D

I

A

K

A

G

Q

Q

Q

H

H

L

A

S

A

D

D

A

F

F

A

A

R

Q

L

V

S

N

L

P

T

L

Q

K

R

K

V

V

P

P

T

A

L

L

V

K

E

D

G

G

D

D

F

F

A

T

L

L

S

T

E

E

S

S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

E

T

Q

R

A

K

Y

Y

P

K

G

V

T

P

P

D

V

Y

-

W

Y

Y

P

P

A

Q

D

D

P

L

K

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

V

Y

Q

L

A

A

W

W

-

Q

-

H

-

T

L

L

R

R

S

R

D

S

L

C

L

L

P

R

I

A

R

L

Q

W

E

H

R

K

S

V

T

M

M

F

V

P

V

V

F

F

Y

L

G

G

Q

E

K

P

M

V

P

S

P

P

L

Q

S

T

P

L

V

A

A

A

E

T

A

L

A

A

A

E

A

L

K

D

L

V

-

T

I

L

S

Q

A

L

Q

E

D

D

L

K

L

F

V

L

G

Q

N

N

P

K

A

A

Y

F

L

L

F

T

G

G

-

P

D

H

W

I

S

S

I

L

A

A

D

D

V

L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
169 V → I: in dbSNP:rs2266637
176 H→Q: Increases activity towards alkylhalogenides, but not hydroperoxides.
234 W→R: Facilitates binding of substrates and increases catalytic activity.

6tahB Glutathione S-transferase
29% identity, 75% coverage: 8:161/205 of query aligns to 6:161/213 of 6tahB

query
sites
6tahB

M

L

Y

Y

T

T

-

A

S
x
T

P

P

Y
x
N

A

G

L

H

S

K

V

V

F

S

V

I

T

A

L

L

R

E

E

E

K

M

G

G

L

L

A

P

F

Y

D

T

T

V

I

H

T

A

L

L

D

S

L

F

D

D

A

K

Q

E

Q
|
Q

H

K

A

A

A

P

D

E

F

F

A

L

R

R

L

I

S

N

L

P

T

N

Q

G

R
|
R

V
x
I

P

P

T

A

L

I

V

V

E

D

-

R

-

S

-

N

G

D

D

D

F

F

A

A

L

V

S

F

E
|
E

S
|
S

S

G

A

A

I

I

T

L

E

V

Y

Y

L

L

E

-

Q

-

A

A

Y

E

P

K

G

T

T

G

P

Q

V

L

Y

M

P

P

A

T

D

D

P

V

K

K

L

G

R

R

A

S

R

R

A

V

R

I

Q

Q

V

W

Q

L

A

M

W

F

L

Q

R
x
M

S

G

D

G

L

V

L
x
G

P
|
P

I

M

R

Q

Q

G

E

Q

R

A

S

N

T

V

M

F

V

F

V

R

F

Y

Y

F

G

P

Q

E

K

K

M

L

P

Q

P

G

L

A

S

I

P

D

V

R

A

Y

E

Q

A

H

A

E

A

T

A

R

K

R

L

L

I

Y

S

E

A

V

A

L

Q

-

D

D

L

G

L

R

V

L

G

G

N

E

P

A

A

E

Y

Y

L

L

F

A

G

G

D

D

W

Y

S

S

I

I

A

A

D

D

V

I

binding oxidized glutathione disulfide: T11 (≠ S11), N13 (≠ Y13), Q40 (= Q40), R53 (= R53), I54 (≠ V54), E69 (= E66), S70 (= S67), M103 (≠ R102), G107 (≠ L106), P108 (= P107)

Sites not aligning to the query:

binding oxidized glutathione disulfide: 166, 213

2c3qA Human glutathione-s-transferase t1-1 w234r mutant, complex with s- hexylglutathione (see paper)
30% identity, 79% coverage: 1:161/205 of query aligns to 2:167/239 of 2c3qA

query
sites
2c3qA

M

L

R

E

L

L

Y

Y

V

L

D

D

H

-

M

L

Y

L

T
x
S

S

Q

P

P

Y

-

A

C

L

R

S

A

V

V

F

Y

V

I

T

F

L

A

R

K

E

K

K

N

G

D

L

I

A

P

F

F

D

E

T

L

I

R

T

I

L

V

D

D

L

L

D

I

A

K

A

G

Q

Q

Q
x
H

H

L

A

S

A

D

D

A

F

F

A

A

R

Q

L

V

S

N

L

P

T

L

Q

K

R
x
K

V
|
V

P

P

T

A

L

L

V

K

E

D

G

G

D

D

F

F

A

T

L

L

S

T

E
|
E

S
|
S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

E

T

Q

R

A

K

Y

Y

P

K

G

V

T

P

P

D

V

Y

-

W

Y

Y

P

P

A

Q

D

D

P

L

K

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

V

Y

Q

L

A

A

W

W

-

Q

-

H

-

T

L

L

R

R

S

R

D

S

L

C

L

L

P

R

I

A

R

L

Q
x
W

E

H

R

K

S

V

T

M

M

F

V

P

V

V

F

F

Y

L

G

G

Q

E

K

P

M

V

P

S

P

P

L

Q

S

T

P

L

V

A

A

A

E

T

A

L

A

A

A

E

A

L

K

D

L

V

-

T

I

L

S

Q

A

L

Q

E

D

D

L

K

L

F

V

L

G

Q

N

N

P

K

A

A

Y

F

L

L

F

T

G

G

-

P

D

H

W

I

S

S

I

L

A

A

D

D

V

L

active site: S10 (≠ T10)
binding s-hexylglutathione: S10 (≠ T10), H39 (≠ Q40), K52 (≠ R53), V53 (= V54), E65 (= E66), S66 (= S67), W114 (≠ Q110)

Sites not aligning to the query:

binding s-hexylglutathione: 233, 237

4pxoA Crystal structure of maleylacetoacetate isomerase from methylobacteriu extorquens am1 with bound malonate and gsh (target efi-507068)
35% identity, 57% coverage: 9:124/205 of query aligns to 9:125/216 of 4pxoA

query
sites
4pxoA

Y

W

T

R

S
|
S

P

A

Y
x
A

A

A

L

F

S

R

V

V

F

R

V

I

T

A

L

L

R

N

E

L

K

K

G

G

L

I

A

A

F

Y

D

E

T

E

I

V

T

F

L

L

D

D

L

L

D

D

A

A

A

G

Q

D

Q
|
Q

H

H

A

K

A

P

D

D

F

F

A

L

R

A

L

I

S

N

L

P

T

Q

Q

G

R
x
A

V
|
V

P

P

T

A

L

L

V

F

E

D

G

G

D

D

F

G

-

P

A

P

L

L

S

T

E
x
Q

S
|
S

S

L

A

A

I

I

T

L

E

D

Y

Y

L

L

E

E

Q

E

A

T

Y

R

P

T

G

G

T

V

P

P

V

L

Y

L

P

P

A

E

D

E

P

P

K

R

L

A

R

R

A

A

R

R

A

A

R

R

Q

S

V

L

Q

A

A

Q

W

V

L

V

R

A

S

C

D

D

L

T

L
x
H

P

P

I

L

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Y

Q

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E
x
P

R
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R

S

V

T

R

M

T

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F

V

L

F

M

Y

E

G

N

Q

Y

K

G

M

L

P

P

binding glutathione: S11 (= S11), A13 (≠ Y13), Q40 (= Q40), A53 (≠ R53), V54 (= V54), Q67 (≠ E66), S68 (= S67), H107 (≠ L106), P112 (≠ E111), R113 (= R112)

4xt0A Crystal structure of beta-etherase ligf from sphingobium sp. Strain syk-6 (see paper)
36% identity, 43% coverage: 14:101/205 of query aligns to 14:103/243 of 4xt0A

query
sites
4xt0A

A

S

L

L

S

K

V

P

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L

V

A

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T

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D

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P

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S

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K

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Q
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Q

H
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H

A

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A

D

D

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F

F

A

K

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K

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Q

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V

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P

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A

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H

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D

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K

A

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E

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S

S

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I

T

C

E

E

Y

Y

L

L

E

E

Q

D

A

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Y

F

P

P

-

E

-

S

G

G

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N

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A

A

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P

K

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L

K

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R

A

A

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A

M

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A

K

W

W

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V

binding glutathione: Q40 (= Q40), H41 (= H41), Q53 (≠ R53), V54 (= V54), E66 (= E66), S67 (= S67)

Sites not aligning to the query:

binding glutathione: 13, 149

1fw1A Glutathione transferase zeta/maleylacetoacetate isomerase (see paper)
32% identity, 50% coverage: 9:110/205 of query aligns to 8:111/208 of 1fw1A

query
sites
1fw1A

Y

F

T

R

S
|
S

P
x
S

Y
x
C

A

S

L

W

S

R

V

V

F

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A

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K

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G

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Y

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N

L
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L

-

I

-

K

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D

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G

Q

Q

Q
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Q

H

F

A

S

A

K

D

D

F

F

A

Q

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A

L

L

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N

L

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M

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K

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x
Q

V
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V

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P

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T

L

L

V

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E

I

G

D

D

G

F

I

A

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x
Q

S
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S

S

L

A

A

I

I

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I

E

E

Y

Y

L

L

E

E

Q

E

A

T

Y

R

P

P

G

T

T

P

P

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V

L

Y

L

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P

A

Q

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D

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P

K

K

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K

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R

A

A

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A

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R

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M

V

I

Q

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A

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W

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L

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R

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S

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D

G

L

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L
x
Q

P

P

I

L

R
x
Q

Q
x
N

binding glutathione: S10 (= S11), C12 (≠ Y13), L34 (= L35), Q41 (= Q40), Q54 (≠ R53), V55 (= V54), Q67 (≠ E66), S68 (= S67), N111 (≠ Q110)
binding sulfate ion: S11 (≠ P12), Q107 (≠ L106), Q110 (≠ R109)

Sites not aligning to the query:

4mpgB Crystal structure of human glutathione transferase theta-2, complex with glutathione and unknown ligand, target efi-507257
33% identity, 49% coverage: 1:100/205 of query aligns to 11:109/252 of 4mpgB

query
sites
4mpgB

M

L

R

E

L

L

Y

F

V

L

D

D

H

-

M

L

Y

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x
S

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P

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A
x
S

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L

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K

K

N

G

G

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A

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D

L

L

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K

A

G

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Q

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x
H

H
x
K

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A

K

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F

A

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I

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x
N

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L

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E

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S

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A

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Y

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C

A

K

Y

Y

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A

A

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W

active site: S19 (≠ T10), S22 (≠ A14), I56 (≠ L48), N57 (≠ S49)
binding glutathione: S19 (≠ T10), P21 (= P12), H48 (≠ Q40), K49 (≠ H41), K61 (≠ R53), L62 (≠ V54), E74 (= E66), S75 (= S67)

Sites not aligning to the query:

binding glutathione: 115

3ljrA Glutathione transferase (theta class) from human in complex with the glutathione conjugate of 1-menaphthyl sulfate (see paper)
33% identity, 49% coverage: 1:100/205 of query aligns to 3:101/244 of 3ljrA

query
sites
3ljrA

M

L

R

E

L

L

Y

F

V

L

D

D

H

-

M

L

Y
x
V

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P

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A

S

L

R

S

A

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V

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Y

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I

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F

L

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R

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E

K

K

N

G

G

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A

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F

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L

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K

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Q

Q
x
H

H
x
K

A

S

A

K

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E

F

F

A

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N

L

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K

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L

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D

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G

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D

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F

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E

S
|
S

S

S

A

A

I

I

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E

I

Y

Y

L

L

E

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Q

C

A

K

Y

Y

P

Q

G

-

T

T

P

P

-

D

-

H

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W

Y

Y

P

P

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A

A

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A

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W

W

active site: S11 (≠ T10)
binding 1-menaphthyl glutathione conjugate: V10 (≠ Y9), S11 (≠ T10), Q12 (≠ S11), P13 (= P12), L35 (= L35), H40 (≠ Q40), K41 (≠ H41), K53 (≠ R53), L54 (≠ V54), E66 (= E66), S67 (= S67)
binding sulfate ion: Q12 (≠ S11)

Sites not aligning to the query:

P0CG30 Glutathione S-transferase theta-2B; Glutathione S-transferase theta-2; GST class-theta-2; EC 2.5.1.18 from Homo sapiens (Human) (see 2 papers)
33% identity, 49% coverage: 1:100/205 of query aligns to 3:101/244 of P0CG30

query
sites
P0CG30

M

L

R

E

L

L

Y

F

V

L

D

D

H

-

M

L

Y

V

T

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S

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S

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A

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Y

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K

K

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G

G

L

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A

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F

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D

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T

L

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T

L

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D

L

L

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K

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G

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Q

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H

H
x
K

A

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A

K

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F

F

A

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L

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N

L

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L

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K

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x
L

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P

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T

L

L

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D

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G

D

D

F

F

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I

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L

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E
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E

S
|
S

S

S

A

A

I

I

T

L

E

I

Y

Y

L

L

E

S

Q

C

A

K

Y

Y

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Q

G

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T

T

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P

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D

-

H

V

W

Y

Y

P

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D

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P

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L

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A

A

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A

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W

HK 40:41 (≠ QH 40:41) binding
KL 53:54 (≠ RV 53:54) binding
ES 66:67 (= ES 66:67) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
104:107 binding
139 M → I: in dbSNP:rs1622002

O43708 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Homo sapiens (Human) (see 10 papers)
32% identity, 49% coverage: 9:109/205 of query aligns to 12:114/216 of O43708

query
sites
O43708

Y

F

T

R

S

S

P

S

Y

C

A

S

L

W

S

R

V

V

F

R

V

I

T

A

L

L

R

A

E

L

K

K

G

G

L

I

A

D

F

Y

D
x
K

T

T

I

V

T

P

L

I

D

N

L

L

-

I

-

K

D

D

A
x
R

A

G

Q

Q

Q
|
Q

H

F

A

S

A

K

D

D

F

F

A

Q

R

A

L

L

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N

L

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M

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V

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P

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L

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D

D

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I

A

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S

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S

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A

A

I

I

T

I

E

E

Y

Y

L

L

E

E

Q

E

A
x
M

Y

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P

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P

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Y

L

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P

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P

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K

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A

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A
x
V

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Q

K32 (≠ D29) to E: in allele GSTZ1*C; dbSNP:rs7975
R42 (≠ A37) to G: in allele GSTZ1*B and allele GSTZ1*C; dbSNP:rs7972
Q45 (= Q40) binding
V59 (= V54) binding
M82 (≠ A77) to T: in dbSNP:rs1046428
V99 (≠ A94) to M: in MAAID; decreased maleylacetoacetate isomerase activity
Q111 (≠ L106) binding

Sites not aligning to the query:

133 N → H: in dbSNP:rs2234955
150 A → V: in MAAID; decreased maleylacetoacetate isomerase activity

Query Sequence

>GFF3010 FitnessBrowser__WCS417:GFF3010
MRLYVDHMYTSPYALSVFVTLREKGLAFDTITLDLDAAQQHAADFARLSLTQRVPTLVEG
DFALSESSAITEYLEQAYPGTPVYPADPKLRARARQVQAWLRSDLLPIRQERSTMVVFYG
QKMPPLSPVAEAAAAKLISAAQDLLVGNPAYLFGDWSIADVDLAVMLNRLILNGDSVPAE
LVEYAQRQWQRPSVQAWVNQHRPAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory