SitesBLAST
Comparing GFF3028 FitnessBrowser__WCS417:GFF3028 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 7 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
25% identity, 79% coverage: 23:356/422 of query aligns to 24:344/383 of 5i39A
- active site: F66 (≠ N65), Q69 (= Q68), A70 (≠ V69), Q248 (≠ V264), P267 (vs. gap)
- binding flavin-adenine dinucleotide: V30 (= V29), G31 (= G30), G33 (= G32), I34 (≠ Y33), L35 (≠ T34), V53 (≠ L52), E54 (= E53), K55 (≠ A54), Q62 (≠ A61), S63 (= S62), F66 (≠ N65), Y67 (≠ G66), Q69 (= Q68), A196 (= A202), A197 (≠ V203), G226 (= G237), G227 (≠ N238), W229 (= W240), Q248 (≠ V264), Q250 (≠ E266), G321 (= G335), M323 (≠ I337)
Sites not aligning to the query:
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 84% coverage: 23:376/422 of query aligns to 1:341/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ V29), G8 (= G30), G10 (= G32), V11 (≠ Y33), I12 (≠ T34), V30 (≠ L52), E31 (= E53), K32 (vs. gap), E38 (= E56), A39 (≠ P57), S40 (≠ G58), A43 (= A61), G45 (= G63), L46 (≠ R64), V171 (= V203), G200 (≠ T232), G201 (≠ N233), W203 (≠ Y235), G298 (= G335), R300 (≠ I337), P301 (≠ A338), Y326 (≠ N361), R327 (≠ G362), N328 (≠ R363), G329 (= G364), I330 (= I365)
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
24% identity, 71% coverage: 23:320/422 of query aligns to 16:298/433 of 5hxwA
- active site: F58 (≠ N65), Q61 (= Q68), A62 (≠ V69), Q240 (≠ V264), V284 (≠ S306), F288 (= F310)
- binding cetyl-trimethyl-ammonium: G291 (≠ L313), Y294 (≠ S316)
- binding flavin-adenine dinucleotide: V22 (= V29), G23 (= G30), G25 (= G32), I26 (≠ Y33), L27 (≠ T34), E46 (= E53), K47 (≠ A54), E53 (≠ G60), Q54 (≠ A61), S55 (= S62), R57 (= R64), F58 (≠ N65), Y59 (≠ G66), G60 (= G67), Q61 (= Q68), A188 (= A202), A189 (≠ V203), G218 (= G237), G219 (≠ N238), W221 (= W240), Q240 (≠ V264), Q242 (≠ E266)
Sites not aligning to the query:
- binding cetyl-trimethyl-ammonium: 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 96% coverage: 3:408/422 of query aligns to 12:412/824 of Q8GAI3
- W66 (≠ G63) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ R64) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
3pvcA Crystal structure of apo mnmc from yersinia pestis (see paper)
26% identity, 49% coverage: 25:232/422 of query aligns to 236:434/635 of 3pvcA
- binding flavin-adenine dinucleotide: I240 (≠ V29), G241 (= G30), G242 (≠ A31), G243 (= G32), I244 (≠ Y33), V245 (≠ T34), A265 (= A54), D266 (≠ N55), A273 (= A61), S274 (= S62), N276 (≠ R64), A280 (≠ Q68), E404 (≠ A202), L405 (≠ V203), T434 (= T232)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 437, 454, 456, 477, 538, 588, 589, 590, 591, 592, 593
3sglA The crystal structure of mnmc from yersinia pestis bound with fad and sam (see paper)
26% identity, 49% coverage: 25:232/422 of query aligns to 233:430/630 of 3sglA
- binding flavin-adenine dinucleotide: I237 (≠ V29), G238 (= G30), G239 (≠ A31), G240 (= G32), I241 (≠ Y33), V242 (≠ T34), A262 (= A54), D263 (≠ N55), A270 (= A61), S271 (= S62), N273 (≠ R64), A277 (≠ Q68), E401 (≠ A202), L402 (≠ V203), T430 (= T232)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 450, 473, 534, 583, 584, 585, 586, 587, 588
- binding s-adenosylmethionine: 33, 34, 35, 36, 37, 38, 39, 68, 69, 123, 124, 145, 146, 147
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
24% identity, 91% coverage: 25:407/422 of query aligns to 21:390/403 of 2gagB
- active site: A61 (vs. gap), T64 (≠ G66), T65 (≠ G67)
- binding flavin-adenine dinucleotide: G26 (= G30), G28 (= G32), G29 (≠ Y33), H30 (≠ T34), E51 (= E53), K52 (≠ A54), G58 (= G63), N59 (vs. gap), M60 (vs. gap), R62 (= R64), N63 (= N65), T64 (≠ G66), I66 (≠ Q68), V195 (= V203), G224 (≠ T232), A225 (≠ N233), H227 (≠ Y235), L231 (= L239), L246 (= L260), G352 (≠ N361), T353 (≠ G362), G354 (≠ R363), G355 (= G364), F356 (≠ I365), K357 (≠ A366)
- binding flavin mononucleotide: A61 (vs. gap), R62 (= R64), H171 (≠ P179), V250 (= V264), E278 (≠ R292), R321 (≠ Y331), W323 (= W333)
- binding 2-furoic acid: T64 (≠ G66), I66 (≠ Q68), R68 (≠ N70), M263 (≠ T277), Y270 (= Y284), K357 (≠ A366)
- binding sulfite ion: K170 (≠ Q178), K276 (≠ H290)
Sites not aligning to the query:
Query Sequence
>GFF3028 FitnessBrowser__WCS417:GFF3028
MKSLWSATAPSVVPTPALRESVKVDVAIVGAGYTGLSTALHLAERGVSVCVLEANEPGWG
ASGRNGGQVNPTLKYDPEQLVQMFGPERAEPLISTVSSSADLVFKLIEKHGIDCAPVRKG
WMQVSYTEKGVAGLHARADQWARRGVPVQRLDASTVASRMGSDAFAGGWLDGRAGAIQPL
AYARGLVGAALAAGVRIHGQSAVTDLRRQGAGWQLQTASGARVMADQVVLATNGYSGNLW
PGMAQSVLAANSFIVATTPLSGSVAERILPGQETVSTAQRLLLYFRKDSHGRLLMGGRGL
FNDPTSPTDFAHLERSLALLFPQLGPLQFEYRWAGRIAITRDFMPHVHQPAPGLTLALGC
NGRGIALCTSLGQQLAGKLCDSQAEFAYPVTPLQRLPMHGLQRFYIGAGVAWYSLLDRLN
IV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory