Comparing GFF3033 FitnessBrowser__Phaeo:GFF3033 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 4 hits to proteins with known functional sites (download)
P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
32% identity, 69% coverage: 101:320/320 of query aligns to 62:280/281 of P94530
P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
23% identity, 66% coverage: 110:320/320 of query aligns to 80:295/296 of P68183
4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
32% identity, 28% coverage: 207:295/320 of query aligns to 380:479/490 of 4ki0F
Sites not aligning to the query:
P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
32% identity, 28% coverage: 207:295/320 of query aligns to 395:494/514 of P02916
Sites not aligning to the query:
>GFF3033 FitnessBrowser__Phaeo:GFF3033
MTRDWWKHLILILGVIVVIAPFYMMVSYSFKSPGEIDRGEGGFFGRQEMMVDERCVKLRD
PNRKDVAAAAHRFPGASDGEIRDALVAEAKADCAMRPVVFNYSKAFTEAPLLRYLLNGII
VTGSIFLIQVVVALPAAYALAKLKFWGREAVFGLVLFCLLIPVHAIALPLYIMLAKLGLT
NTYAALVVPWTISVFGIFLMRQFFMTVPDDLIDAARMDGMGEFSIVWRVMLPTAIPALLA
FAIFSIVAHWNDYFWPRIVVTGNRDLFTPPLGLREFKGDGDGSYFGPMMATATVIVTPLI
VAFLLAQKRFIEGITLSGMK
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory