SitesBLAST
Comparing GFF305 FitnessBrowser__psRCH2:GFF305 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3du4A Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8- diaminopelargonic acid synthase in bacillus subtilis (see paper)
42% identity, 92% coverage: 15:444/467 of query aligns to 13:440/448 of 3du4A
- active site: F17 (≠ C19), Y146 (= Y149), E217 (= E221), D251 (= D255), A254 (= A258), K280 (= K284), A417 (≠ Y421)
- binding 7-keto-8-aminopelargonic acid: L82 (≠ A85), Y146 (= Y149), G315 (≠ S319), S317 (≠ T321), R410 (= R414)
- binding pyridoxal-5'-phosphate: S112 (≠ N115), G113 (= G116), A114 (≠ S117), Y146 (= Y149), H147 (= H150), E217 (= E221), D251 (= D255), V253 (≠ I257), A254 (= A258), K280 (= K284), H316 (= H320), S317 (≠ T321)
P53555 L-Lysine--8-amino-7-oxononanoate transaminase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; L-Lysine--8-amino-7-oxononanoate aminotransferase; EC 2.6.1.105 from Bacillus subtilis (strain 168) (see paper)
42% identity, 92% coverage: 15:444/467 of query aligns to 13:440/448 of P53555
- GA 113:114 (≠ GS 116:117) binding pyridoxal 5'-phosphate
- Y146 (= Y149) binding substrate
- K280 (= K284) modified: N6-(pyridoxal phosphate)lysine
- G315 (≠ S319) binding substrate
- HS 316:317 (≠ HT 320:321) binding pyridoxal 5'-phosphate
- R410 (= R414) binding substrate
6wnnA Bacillus subtilis bioa in complex with amino donor l-lys
40% identity, 92% coverage: 15:444/467 of query aligns to 10:412/420 of 6wnnA
- active site: F14 (≠ C19), Y143 (= Y149), D223 (= D255), K252 (= K284)
- binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: F14 (≠ C19), W51 (= W57), S109 (≠ N115), G110 (= G116), A111 (≠ S117), Y143 (= Y149), H144 (= H150), D223 (= D255), V225 (≠ I257), K252 (= K284), R382 (= R414)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: H288 (= H320), S289 (≠ T321)
6zhkA Crystal structure of adenosylmethionine-8-amino-7-oxononanoate aminotransferase from methanocaldococcus jannaschii dsm 2661
39% identity, 96% coverage: 2:447/467 of query aligns to 3:436/438 of 6zhkA
3dodA Crystal structure of plp bound 7,8-diaminopelargonic acid synthase in bacillus subtilis (see paper)
39% identity, 92% coverage: 15:444/467 of query aligns to 11:409/417 of 3dodA
- active site: F15 (≠ C19), E186 (= E221), D220 (= D255), A223 (= A258), K249 (= K284), A386 (≠ Y421)
- binding pyridoxal-5'-phosphate: W52 (= W57), S110 (≠ N115), G111 (= G116), A112 (≠ S117), D220 (= D255), V222 (≠ I257), K249 (= K284), H285 (= H320), S286 (≠ T321)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
41% identity, 92% coverage: 15:444/467 of query aligns to 13:421/429 of 6ed7A
- active site: Y17 (≠ C19), Y144 (= Y149), D245 (= D255), K274 (= K284)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (≠ C19), W52 (= W56), W52 (= W56), Y144 (= Y149), D147 (≠ E152), A217 (= A227), K274 (= K284), R391 (= R414), F393 (≠ L416), F393 (≠ L416)
- binding pyridoxal-5'-phosphate: G112 (= G116), S113 (= S117), Y144 (= Y149), H145 (= H150), D245 (= D255), I247 (= I257), K274 (= K284)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
41% identity, 93% coverage: 15:448/467 of query aligns to 13:425/429 of P12995
- Y17 (≠ C19) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W56) binding substrate
- GS 112:113 (= GS 116:117) binding pyridoxal 5'-phosphate
- Y144 (= Y149) mutation to F: Severely reduces the aminotransferase activity.
- D147 (≠ E152) mutation to N: Loss of aminotransferase activity.
- D245 (= D255) binding pyridoxal 5'-phosphate
- R253 (= R263) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K284) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (≠ S319) binding substrate
- PT 308:309 (≠ HT 320:321) binding pyridoxal 5'-phosphate
- R391 (= R414) binding substrate; mutation to A: Reduces aminotransferase activity.
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
41% identity, 92% coverage: 15:444/467 of query aligns to 13:421/429 of 1dtyA
- active site: Y17 (≠ C19), Y144 (= Y149), E211 (= E221), D245 (= D255), A248 (= A258), K274 (= K284), Y398 (= Y421)
- binding pyridoxal-5'-phosphate: G112 (= G116), S113 (= S117), Y144 (= Y149), H145 (= H150), D245 (= D255), I247 (= I257), K274 (= K284)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
40% identity, 92% coverage: 17:444/467 of query aligns to 15:420/427 of 1mlzA
- active site: Y17 (≠ C19), Y144 (= Y149), E210 (= E221), D244 (= D255), A247 (= A258), K273 (= K284), Y397 (= Y421)
- binding pyridoxal-5'-phosphate: G112 (= G116), S113 (= S117), Y144 (= Y149), H145 (= H150), D244 (= D255), I246 (= I257), K273 (= K284), P307 (≠ H320), T308 (= T321)
- binding trans-amiclenomycin: W52 (= W56), W53 (= W57), Y144 (= Y149), K273 (= K284), R390 (= R414), F392 (≠ L416)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
40% identity, 92% coverage: 17:444/467 of query aligns to 15:420/427 of 1mlyA
- active site: Y17 (≠ C19), Y144 (= Y149), E210 (= E221), D244 (= D255), A247 (= A258), K273 (= K284), Y397 (= Y421)
- binding cis-amiclenomycin: W52 (= W56), W53 (= W57), K273 (= K284), R390 (= R414), F392 (≠ L416)
- binding pyridoxal-5'-phosphate: G112 (= G116), S113 (= S117), Y144 (= Y149), H145 (= H150), D244 (= D255), I246 (= I257), K273 (= K284), P307 (≠ H320), T308 (= T321)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
40% identity, 92% coverage: 15:444/467 of query aligns to 13:409/416 of 1qj3A
- active site: Y17 (≠ C19), Y144 (= Y149), E201 (= E221), D235 (= D255), A238 (= A258), K264 (= K284), Y386 (= Y421)
- binding 7-keto-8-aminopelargonic acid: Y17 (≠ C19), W52 (= W56), Y144 (= Y149), K264 (= K284), R379 (= R414), F381 (≠ L416)
- binding pyridoxal-5'-phosphate: G112 (= G116), S113 (= S117), Y144 (= Y149), H145 (= H150), G146 (= G151), D235 (= D255), I237 (= I257), A238 (= A258), K264 (= K284)
6erkA Crystal structure of diaminopelargonic acid aminotransferase from psychrobacter cryohalolentis (see paper)
38% identity, 90% coverage: 15:436/467 of query aligns to 14:405/420 of 6erkA
3tfuA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, post-reaction complex with a 3,6- dihydropyrid-2-one heterocycle inhibitor (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 11:412/417 of 3tfuA
- active site: Y19 (≠ C19), Y151 (= Y149), E204 (= E221), D238 (= D255), A241 (= A258), K267 (= K284), Y391 (= Y421)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-2-oxo-1,2-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: W58 (= W56), G118 (= G116), S119 (= S117), Y151 (= Y149), H152 (= H150), D238 (= D255), I240 (= I257), K267 (= K284), P301 (≠ H320), T302 (= T321)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 10:420/427 of 4cxqA
- active site: Y18 (≠ C19), Y149 (= Y149), E212 (= E221), D246 (= D255), A249 (= A258), K275 (= K284), Y399 (= Y421)
- binding 7-keto-8-aminopelargonic acid: W56 (= W56), Y149 (= Y149), G308 (≠ S319), T310 (= T321), R392 (= R414)
- binding pyridoxal-5'-phosphate: G116 (= G116), S117 (= S117), Y149 (= Y149), H150 (= H150), G151 (= G151), E212 (= E221), D246 (= D255), I248 (= I257), K275 (= K284), P309 (≠ H320), T310 (= T321)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 10:418/425 of 4w1vA
- active site: Y18 (≠ C19), Y147 (= Y149), E210 (= E221), D244 (= D255), A247 (= A258), K273 (= K284), Y397 (= Y421)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P18), Y18 (≠ C19), W54 (= W56), M81 (= M83), G83 (≠ A85), Y147 (= Y149), G306 (≠ S319), P307 (≠ H320), T308 (= T321), F392 (≠ L416)
- binding pyridoxal-5'-phosphate: G114 (= G116), S115 (= S117), Y147 (= Y149), H148 (= H150), E210 (= E221), D244 (= D255), I246 (= I257), K273 (= K284)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 10:418/425 of 4cxrA
- active site: Y18 (≠ C19), Y147 (= Y149), E210 (= E221), D244 (= D255), A247 (= A258), K273 (= K284), Y397 (= Y421)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (≠ C19), W54 (= W56), W55 (= W57), A216 (= A227)
- binding pyridoxal-5'-phosphate: G114 (= G116), S115 (= S117), Y147 (= Y149), H148 (= H150), E210 (= E221), D244 (= D255), I246 (= I257), K273 (= K284), P307 (≠ H320), T308 (= T321)
4wyaA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a fragment hit (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 11:415/420 of 4wyaA
- active site: Y19 (≠ C19), Y151 (= Y149), E209 (= E221), D243 (= D255), A246 (= A258), K272 (= K284), Y394 (= Y421)
- binding 5-(pyridin-2-yl)thiophene-2-carboxamide: P18 (= P18), W58 (= W56), F389 (≠ L416)
- binding pyridoxal-5'-phosphate: S117 (≠ N115), G118 (= G116), S119 (= S117), Y151 (= Y149), H152 (= H150), E209 (= E221), D243 (= D255), I245 (= I257), K272 (= K284), P304 (≠ H320), T305 (= T321)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 17:428/437 of P9WQ81
- Y25 (≠ C19) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W56) binding S-adenosyl-L-methionine
- Y157 (= Y149) binding S-adenosyl-L-methionine
- K283 (= K284) modified: N6-(pyridoxal phosphate)lysine
- G316 (≠ S319) binding S-adenosyl-L-methionine
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 10:421/428 of 5te2A
- active site: Y18 (≠ C19), Y150 (= Y149), E213 (= E221), D247 (= D255), A250 (= A258), K276 (= K284), Y400 (= Y421)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (≠ C19), W57 (= W56), G117 (= G116), S118 (= S117), Y150 (= Y149), H151 (= H150), G152 (= G151), D247 (= D255), I249 (= I257), K276 (= K284), G309 (≠ S319), P310 (≠ H320), T311 (= T321)
5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
38% identity, 93% coverage: 11:442/467 of query aligns to 10:421/429 of 5kgtA
- active site: Y18 (≠ C19), Y150 (= Y149), E213 (= E221), D247 (= D255), A250 (= A258), K276 (= K284), Y400 (= Y421)
- binding 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1-yl]phenyl]ethanone: M84 (= M83), G86 (≠ A85), G309 (≠ S319), T311 (= T321)
- binding pyridoxal-5'-phosphate: S116 (≠ N115), G117 (= G116), S118 (= S117), Y150 (= Y149), H151 (= H150), G152 (= G151), E213 (= E221), D247 (= D255), I249 (= I257), K276 (= K284)
Query Sequence
>GFF305 FitnessBrowser__psRCH2:GFF305
MSLNDSWMQRDLAVLWHPCTQMKDHEQLPLIPIRRGDGVWLEDFDGKRYLDAVSSWWVNV
FGHANPRINQRIKDQVDQLEHVMLAGFSHQPVVELSERLVALTPAGLDRVFYTDNGSTGI
EVALKMSFHYWRNSGRGQKQRFVTLTNSYHGETVAAMSVGDVALFTDTYKPLLLDTFKVP
SPDCYLRPDGVSWEEHSRQMFAHMEQTLAEHHQDIAAVIVEPLIQGAGGMRMYHPVYLKL
LREACDRYEVHLIHDEIAVGFGRTGTMFACEQAGITPDFLCLSKALTGGYLPMAAVLTTD
RIYQAFYDDYSTLRAFLHSHTYTGNPLACAAALATLDIFAEDNVIEANKPLAARMASATA
HLVDHPHVAEVRQTGMALAIEMVQDKASRTAYPWQERRGLKVYQHALERGALLRPLGSVV
YFLPPYCITEEQIDFLAAVATEGIDIATRETSVSVASGSYPGFRDPG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory