SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3079 FitnessBrowser__Marino:GFF3079 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/183 of 5odqI

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5odqI

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binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), T39 (≠ F30), C40 (= C31), S43 (≠ T34), C44 (= C35), P45 (= P36), Y52 (≠ P48), T54 (≠ G50), R55 (= R51), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), R89 (≠ S87), V91 (= V89), T94 (≠ G92), I97 (≠ V95)

Sites not aligning to the query:

binding 2-bromanylethanesulfonic acid: 117, 128

5odhI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/183 of 5odhI

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5odhI

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binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), T39 (≠ F30), C40 (= C31), C44 (= C35), Y52 (≠ P48), T54 (≠ G50), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), V91 (= V89)

Sites not aligning to the query:

binding Coenzyme B: 117, 128

5odcC Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/184 of 5odcC

query
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5odcC

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binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), T39 (≠ F30), C40 (= C31), C44 (= C35), P45 (= P36), Y52 (≠ P48), T54 (≠ G50), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), P88 (= P86), V91 (= V89), T94 (≠ G92)

5odcI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/173 of 5odcI

query
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5odcI

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binding magnesium ion: R53 (= R49), D73 (≠ H71)
binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), T39 (≠ F30), C40 (= C31), C44 (= C35), P45 (= P36), Y52 (≠ P48), T54 (≠ G50), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), V91 (= V89), T94 (≠ G92)

Query Sequence

>GFF3079 FitnessBrowser__Marino:GFF3079
MQTNLVQQFANTKEGQEAESILRACVHCGFCTATCPTYQELNDERDGPRGRIYLMKMFLE
GAEVTEKTREHLDRCLTCRSCETTCPSGVQYGRLVDISRGLMEKEMPREPKDKWLRWALA
RVIPNRQLFGVLLRLGQVFRPVLPEKLRTKVPPRKQASPWPAASHSRIVLALAGCVQPSA
TPNTNAAAARVLDRLGITMVEAPEAGCCGAVNYHLSEHEKGLERMRQNIDAWWPAIEAGA
EAIIMTASGCGAMVQDYGHLLKDDPVYAAKAQKVSELCTDLGAFLLKQDLEKLKVRQDPG
KVAFHCPCTLQHAMKQNGVVEQVLTRAGVNLAATKDKHLCCGSAGTYSVTQPEMSQKLLG
NKLKALTVDNPDRIVTANIGCQMHLETKSPVPVQHWIELLDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory