SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3079 FitnessBrowser__Marino:GFF3079 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

5odcC Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/184 of 5odcC

query
sites
5odcC

G

G

Q

D

E

D

A

V

E

V

S

S

I

S

L

L

R

K

A

A

C
|
C

V
x
Y

H
x
Q

C
|
C

G
|
G

F

T

C
|
C

T

T

A

G

T

S

C
|
C

P
|
P

T

S

Y

-

Q

-

E

-

L

-

N

-

D

G

E

R

R

R

D

T

G

S

P
x
Y

R

R

G
x
T

R

R

I

K

Y

V

L

I

M

R

K

K

M

A

F

L

L

L

E

G

G

M

A

D

E

D

V

V

T

L

E

D

K

S

T

-

R

-

E

D

H

D

L

I

D

W

R

K

C
|
C

L
x
T

T
|
T

C
|
C

R
x
Y

S

T

C
|
C

E

Y

T

E

T

R

C
|
C

P
|
P

S

R

G

D

V
|
V

Q

K

Y

V

G

T

R

E

L

I

V

I

D

K

I

T

S

I

R

R

G

N

L

L

binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), C40 (= C31), C44 (= C35), P45 (= P36), Y52 (≠ P48), T54 (≠ G50), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), P88 (= P86), V91 (= V89)

5odcI Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
30% identity, 22% coverage: 15:101/402 of query aligns to 24:103/173 of 5odcI

query
sites
5odcI

G

G

Q

D

E

D

A

V

E

V

S

S

I

S

L

L

R

K

A

A

C
|
C

V
x
Y

H
x
Q

C
|
C

G
|
G

F

T

C
|
C

T

T

A

G

T

S

C
|
C

P
|
P

T

S

Y

-

Q

-

E

-

L

-

N

-

D

G

E

R

R

R

D

T

G

S

P
x
Y

R
|
R

G
x
T

R

R

I

K

Y

V

L

I

M

R

K

K

M

A

F

L

L

L

E

G

G

M

A

D

E

D

V

V

T

L

E

D

K

S

T

-

R

-

E

D

H
x
D

L

I

D

W

R

K

C
|
C

L
x
T

T
|
T

C
|
C

R
x
Y

S

T

C
|
C

E

Y

T

E

T

R

C
|
C

P

P

S

R

G

D

V
|
V

Q

K

Y

V

G

T

R

E

L

I

V

I

D

K

I

T

S

I

R

R

G

N

L

L

binding magnesium ion: R53 (= R49), D73 (≠ H71)
binding iron/sulfur cluster: C34 (= C25), Y35 (≠ V26), Q36 (≠ H27), C37 (= C28), G38 (= G29), C40 (= C31), C44 (= C35), P45 (= P36), Y52 (≠ P48), T54 (≠ G50), C77 (= C75), T78 (≠ L76), T79 (= T77), C80 (= C78), Y81 (≠ R79), C83 (= C81), C87 (= C85), V91 (= V89)

Query Sequence

>GFF3079 FitnessBrowser__Marino:GFF3079
MQTNLVQQFANTKEGQEAESILRACVHCGFCTATCPTYQELNDERDGPRGRIYLMKMFLE
GAEVTEKTREHLDRCLTCRSCETTCPSGVQYGRLVDISRGLMEKEMPREPKDKWLRWALA
RVIPNRQLFGVLLRLGQVFRPVLPEKLRTKVPPRKQASPWPAASHSRIVLALAGCVQPSA
TPNTNAAAARVLDRLGITMVEAPEAGCCGAVNYHLSEHEKGLERMRQNIDAWWPAIEAGA
EAIIMTASGCGAMVQDYGHLLKDDPVYAAKAQKVSELCTDLGAFLLKQDLEKLKVRQDPG
KVAFHCPCTLQHAMKQNGVVEQVLTRAGVNLAATKDKHLCCGSAGTYSVTQPEMSQKLLG
NKLKALTVDNPDRIVTANIGCQMHLETKSPVPVQHWIELLDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory