SitesBLAST

C9 (≠ F9) mutation to S: No effect.
C20 (= C20) mutation to S: Loss of oligomerization; no alteration of water permeability.
T183 (= T181) mutation to C: No effect.
R189 (= R187) mutation R->V,S: Loss of function.

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
63% identity, 90% coverage: 1:227/251 of query aligns to 3:231/232 of 2o9eA

query
sites
2o9eA

M

M

Q

F

K

R

R

K

L

L

T

A

A

A

E

E

F

S

I

F

G

G

T

T

F

F

W

W

L

L

T

V

F

F

G

G

C

S

G

G

S

S

A

A

I

V

L

L

A

A

A

G

F

F

P

P

E

E

L

L

G

G

I

I

G

G

F

F

V

A

G

G

V

V

S

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

W

W

A

A

G

G

R

G

R

R

V

F

A

P

A

A

G

K

E

E

V

V

L

V

P

G

Y

Y

I

V

A

I

A

A

Q

Q

V

V

A

V

G

G

A

G

I

I

G

V

A

A

S

A

A

A

A

L

L

L

Y

Y

L

L

I

I

A

A

N

S

G

G

Q

K

P

T

D

G

F

F

-

D

-

A

A

A

I

A

G

S

G

G

F

F

A

A

A

S

N

N

G

G

Y

Y

G

G

P

E

L

H

S

S

P

P

G

G

L

G

F

Y

D

S

M

M

K

L

A

S

A

A

L

L

L

V

A

V

E
|
E

C

L

I

V

A

L

T

S

F

A

F

G

F

F

L

L

F

L

I

V

I

I

M

H

R

G

V

A

T

T

S

D

S

K

G

F

A

A

V

P

P

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

L

L

A

A

L

L

T

T

L

L

I

I

H

H

L

L

V

I

L
x
S

I

I

P

P

V

V

T

T

N

N

T

C

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

P

V

A

A

L

I

F

F

A

Q

G

G

E

W

Y

A

L

L

A

E

Q

Q

L

L

W

W

L

F

F

F

W

W

L

V

A

V

P

P

M

I

V

V

G

G

V

I

M

I

G

G

A

G

L

L

A

I

A

Y

R

R

S

T

L

L

G

L

E

E

R

K

binding mercury (ii) ion: E140 (= E136), S179 (≠ L175)

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
65% identity, 82% coverage: 1:207/251 of query aligns to 3:211/230 of 3nkaA

query
sites
3nkaA

M

M

Q

F

K

R

R

K

L

L

T

A

A

A

E

E

F

C

I

F

G

G

T

T

F

F

W

W

L

L

T

V

F

F

G

G

C

C

G

G

S

S

A
|
A

I

V

L

L

A

A

A

G

F

F

P

P

E

E

L

L

G

G

I

I

G

G

F

F

V

A

G

G

V
|
V

S

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

W

W

A

A

G

G

R

G

R

R

V

F

A

P

A

A

G

K

E

E

V

V

L

V

P

G

Y

Y

I

V

A

I

A

A

Q

Q

V

V

A

V

G

G

A

G

I

I

G

V

A

A

S

A

A

A

A

L

L

L

Y

Y

L

L

I

I

A

A

N

S

G

G

Q

K

P

T

D

G

F

F

-

D

-

A

A

A

I

A

G

S

G

G

F

F

A

A

A

S

N

N

G

G

Y

Y

G

G

P

E

L

H

S

S

P

P

G

G

L

G

F

Y

D

S

M

M

K

L

A

S

A

A

L

L

L

V

A

V

E

E

C

L

I

V

A

L

T

S

F

A

F

G

F

F

L

L

F

L

I

V

I

I

M

H

R

G

V

A

T

T

S

D

S

K

G

F

A

A

V

P

P

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

L

L

A

A

L

L

T

T

L

L

I

I

H

G

L

L

V

I

L

S

I
|
I

P

P

V

V

T

T

N
|
N

T

F

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

P

V

A

A

L

I

F

F

A

Q

G

G

E

W

Y

A

L

L

A

E

Q

Q

L

L

W

W

L

F

F

F

W

W

binding glycerol: A25 (= A23), V41 (= V39), I180 (= I176), N184 (= N180)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
40% identity, 90% coverage: 3:229/251 of query aligns to 38:252/271 of P08995

query
sites
P08995

K

Q

R

K

L

L

T

V

A

A

E

E

F

A

I

V

G

G

T

T

F

Y

W

F

L

L

T

I

F

F

G

A

G

G

C

C

G

A

S

S

A

L

I

V

L

V

A

N

A

E

A

N

F

Y

P

Y

E

N

L

M

G

-

I

I

G

T

F

F

V

P

G

G

V

I

S

A

L

I

A

V

F

W

G

G

L

L

T

V

V

L

L

T

T

V

M

L

A

V

Y

Y

A

T

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

G

A

L

F

W

A

A

S

G

T

R

R

V

F

A

P

A

L

G

I

E

Q

V

V

L

P

P

A

Y

Y

I

V

A

V

A

A

Q

Q

V

L

A

L

G

G

A

S

I

I

G

L

A

A

S

S

A

G

A

T

L

L

Y

R

L

L

I

L

A

F

N

M

G

G

Q

N

P

H

D

D

F

-

A

Q

I

F

G

S

G

G

F

T

A

V

A

P

N

N

G

G

Y

T

G

N

P

-

L

-

S

-

P

-

G

-

L

-

F

-

D

-

M

-

K

L

A

Q

A

A

L

F

L

V

A

F

E

E

C

F

I

I

A

M

T

T

F

F

F

L

L

M

F

F

I

V

I

I

M

C

R

G

V

V

-

A

T

T

S

D

S

N

G

R

A

A

V

V

P

G

G

E

F

F

A

A

P

G

I

I

A

A

I

I

G

G

L

S

A

T

L

L

T

L

L

L

I

N

H

V

L

I

V

I

L

G

I

G

P

P

V

V

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

P

A

A

L

-

F

F

A

V

G

H

G

G

E

E

Y

Y

L

-

A

E

Q

G

L

I

W

W

L

I

F

Y

W

L

L

L

A

A

P

P

M

V

V

V

G

G

G

A

V

I

M

A

G

G

A

A

L

W

A

V

A

Y

R

N

S

I

L

V

G

R

E

Y

R

T

D

D

K

K

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
37% identity, 86% coverage: 3:219/251 of query aligns to 36:247/323 of P55088

query
sites
P55088

K

K

R

A

L

V

T

S

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

I

L

F

T

V

F

L

G

L

G

G

C

V

G

G

S

S

A

T

I

I

-

N

L

W

A

G

A

S

F

E

P

N

E

P

L

L

G

P

I

V

G

D

F

M

V

V

G

L

V

I

S

S

L

L

A

C

F

F

G

G

L

L

T

S

V

I

L

A

T

T

M

M

A

V

Y

Q

A

C

V

F

G

G

G

H

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L

M

W

V

A

C

G

T

R

R

R

K

V

I

A
x
S

A

I

G

A

E

K

V

S

L

V

P

F

Y

Y

I

I

A

I

A

A

Q

Q

V

C

A

L

G

G

A

A

I

I

G

I

A

G

S

A

A

G

A

I

L

L

Y

Y

L

L

I

V

A

T

N

-

G

-

Q

-

P

P

D

P

F

S

A

V

I

V

G

G

F

L

A

G

A

V

N

T

G

T

-

V

Y

H

G

G

P

N

L

L

S

T

P

A

G

G

L

-

F

-

D

-

M

-

K

-

A

H

A

G

L

L

A

V

E

E

C

L

I

I

A

I

T

T

F

F

F

Q

F

L

L

V

F

F

I

T

I

I

M

F

R

A

V

S

T

C

S

D

S

S

G

K

-

R

-

T

A

D

V

V

P

T

G

G

F

S

A

I

P

A

I

L

A

A

I

I

G

G

L

F

A

S

L

V

T

A

L

I

I

G

H

H

L

L

V

F

L

A

I

I

P

N

V

Y

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

F

I

A

M

G

G

G

N

E

-

Y

-

L

W

A

A

Q

N

L

H

W

W

L

I

F

Y

W

W

L

V

A

G

P

P

M

I

V

M

G

G

G

A

V

V

M

L

-

A

G

G

A

A

L

L

S111 (≠ A77) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
37% identity, 89% coverage: 1:224/251 of query aligns to 4:243/245 of 2evuA

query
sites
2evuA

M

L

Q

T

K

K

R

R

L

C

T

I

A

A

E

E

F

F

I

I

G

G

T

T

F

F

W

I

L

L

T

V

F

F

G

F

G

G

C

A

G

G

S

S

A

A

I

A

L

V

A

T

A

L

A

M

F

I

P

A

E

S

L

G

G

G

I

T

G

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

-

G

-

D

F
x
W

V

V

G

A

V

I

S

G

L

L

A

A

F

F

G

G

L

F

T

A

V
x
I

L

A

T

A

M

S

A

I

Y

Y

A

A

V

L

G

G

G

N

I

I

S

S

G

G

G

C

H

H

F
x
I

N
|
N

P

P

A

A

V

V

T

T

L

I

G

G

L

L

W

W

A

S

G

V

R

K

V

F

A

P

A

G

G

R

E

E

V

V

L

V

P

P

Y

Y

I

I

A

I

A

A

Q

Q

V

L

A

L

G

G

A

A

I

A

G

F

A

G

S

S

A

-

A

F

L

I

Y

F

L

L

I

Q

A

C

N

A

G

G

Q

I

P

G

D

A

F

A

A

T

I

V

G

G

F

L

A
x
G

A
|
A

N

T

G

-

Y

-

G
x
A

P
|
P

L
x
F

S

-

P

P

G

G

L

I

F

-

D

S

M

Y

K

W

A

Q

A

A

L

M

L

L

A

A

E

E

C

V

I

V

A

G

T

T

F

F

F

L

L

M

F

I

I

T

I

I

M

M

R

G

V

I

T

A

S

V

S

D

G

E

A

R

V

A

P

P

-

K

G

G

F

F

A

A

P

G

I

I

A

I

I

I

G

G

L

L

A

T

L

V

T

A

L

G

I

I

H

I

L

T

V

T

L

L

I

G

P

N

V

I

T

S

N

G

T

S

S

S

V

L

N

N

P

P

A

A

R

R

S

T

T

F

G

G

P

P

A

Y

L

L

-

N

-

D

-

M

-

I

F

F

A

A

G

G

G

T

E

D

Y

L

L

W

A

N

Q

Y

L

Y

W

S

L

I

F

Y

W

V

L

I

A

G

P

P

M

I

V

V

G

G

G

A

V

V

M

L

G

A

A

A

L

L

A

T

A

Y

R

Q

S

Y

L

L

binding glycerol: I44 (vs. gap), W55 (≠ F36), I66 (≠ V47), I81 (≠ F62), N82 (= N63), G133 (≠ A115), A134 (= A116), A136 (≠ G120), P137 (= P121), F138 (≠ L122)

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
33% identity, 87% coverage: 1:218/251 of query aligns to 35:263/285 of Q9XF58

query
sites
Q9XF58

M

L

Q

Y

K

R

R

A

L

V

T

I

A

A

E

E

F

F

I

V

G

A

T

T

F

L

W

L

L

F

T

L

F

Y

G

I

G

T

C

V

G

A

S

T

A

V

I

I

-

G

-

Y

-

K

-

H

-

Q

-

T

-

D

L

A

A

A

A

S

F

G

P

P

E

D

L

A

-

A

-

C

-

G

G

G

I

V

G

G

F

V

V

L

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

V

S

S

G

G

G
|
G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

W

F

A

L

G

A

R

R

R

K

V

V

A

S

A

L

G

V

E

R

V

A

L

L

P

L

Y

Y

I

I

A

V

A

A

Q

Q

V

C

A

L

G

G

A

A

I

I

G

-

A

-

S

-

A

C

A

G

L

V

Y

G

L

L

I

V

A

K

N

G

G

F

Q

Q

P

S

D

A

F

F

A

Y

I

V

-

R

-

Y

G

G

F

-

A

-

N

-

G

G

Y

A

G

N

P

E

L

L

S

S

P

A

G

G

L

-

F

Y

D

S

M

K

K

G

A

T

A

G

L

L

L

A

A

A

E

E

C

I

I

I

A

G

T

T

F

F

F

V

F

L

L

V

F

Y

I

T

I

V

M

F

R

S

V

A

T

T

S

D

-

P

-

K

-

R

-

N

-

A

-

R

S

D

G

S

A

H

V

V

P

P

G

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

M

I

V

H

H

L

L

V

A

L

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

-

V

L

I

F

Y

A

N

G

N

G

D

E

K

Y

A

L

W

A

D

Q

D

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

G

A

V

A

M

I

G

A

A

A

G104 (= G60) mutation to W: Loss of water transport.

P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
36% identity, 86% coverage: 3:219/251 of query aligns to 36:247/323 of P47863

query
sites
P47863

K

K

R

A

L

V

T

T

A

A

E

E

F

F

I

L

G

A

T

M

F

L

W

I

L

F

T

V

F

L

G

L

G

S

C

V

G

G

S

S

A

T

I

I

-

N

L

W

A

G

A

S

F

E

P

N

E

P

L

L

G

P

I

V

G

D

F

M

V

V

G

L

V

I

S

S

L

L

A

C

F

F

G

G

L

L

T

S

V

I

L

A

T

T

M

M

A

V

Y

Q

A

C

V

F

G

G

G

H

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L

M

W

V

A

C

G

T

R

R

R

K

V

I

A

S

A

I

G

A

E

K

V

S

L

V

P

F

Y

Y

I

I

A

T

A

A

Q

Q

V

C

A

L

G

G

A

A

I

I

G

I

A

G

S

A

A

G

A

I

L

L

Y

Y

L

L

I

V

A

T

N

-

G

-

Q

-

P

P

D

P

F

S

A

V

I

V

G

G

F

L

A

G

A

V

N

T

G

T

-

V

Y

H

G

G

P

N

L

L

S

T

P

A

G

G

L

-

F

-

D

-

M

-

K

-

A

H

A

G

L

L

A

V

E

E

C

L

I

I

A

I

T

T

F

F

F

Q

F

L

L

V

F

F

I

T

I

I

M

F

R

A

V

S

T

C

S

D

S
|
S

G

K

-

R

-

T

A

D

V

V

P

T

G

G

F

S

A

V

P

A

I

L

A

A

I

I

G

G

L

F

A

S

L

V

T

A

L

I

I

G

H
|
H

L

L

V

F

L

A

I

I

P

N

V

Y

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

F

I

A

M

G

G

G

N

E

-

Y

-

L

W

A

E

Q

N

L

H

W

W

L

I

F

Y

W

W

L

V

A

G

P

P

M

I

V

I

G

G

G

A

V

V

M

L

-

A

G

G

A

A

L

L

S180 (= S153) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.
H201 (= H172) mutation to P: Partial loss of transport activity.

Sites not aligning to the query:

13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
285 modified: Phosphoserine

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 85% coverage: 7:219/251 of query aligns to 56:271/286 of P61837

query
sites
P61837

A

A

E

E

F

F

I

I

G

A

T

T

F

F

W

L

L

F

T

L

F

Y

G

I

G

T

C

V

G

L

S

T

A

V

I

M

L

G

A

V

A

K

A

R

F

S

P

P

E

N

L

M

-

C

-

A

G

S

I

V

G

G

F

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

W

F

A

L

G

A

R

R

R

K

V

L

A

S

A

L

G

T

E

R

V

A

L

L

P

Y

Y

Y

I

I

A

V

A

M

Q

Q

V

C

A

L

G

G

A

A

I

I

G

C

A

G

S

A

A

G

A

-

L

-

Y

-

L

V

I

V

A

K

N

G

G

F

Q

Q

P

P

D

K

-

Q

-

Y

F

Q

A

A

I

L

G

G

F

G

A

A

-

N

-

T

-

V

A

A

N

H

G

G

Y

Y

G

T

P

K

L

G

S

S

P

-

G

-

L

-

F

-

D

-

M

-

K

-

A

-

A

G

L

L

L

G

A

A

E

E

C

I

I

I

A

G

T

T

F

F

F

V

F

L

L

V

F

Y

I

T

I

V

M

F

R

S

V

A

T

T

S

D

-

A

-

K

-

R

-

N

-

A

-

R

S

D

G

S

A

H

V

V

P

P

G

I

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

L

I

V

H

H

L

L

V

A

L

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

L

I

F

I

A

Y

G

N

G

K

E

D

Y

H

-

S

L

W

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

G

A

V

A

M

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 85% coverage: 7:219/251 of query aligns to 56:271/286 of Q08733

query
sites
Q08733

A

A

E

E

F

F

I

I

G

A

T

T

F

F

W

L

L

F

T

L

F

Y

G

I

G

T

C

V

G

L

S

T

A

V

I

M

L

G

A

V

A

K

A

R

F

A

P

P

E

N

L

M

-

C

-

A

G

S

I

V

G

G

F

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

W

F

A

L

G

A

R

R

R

K

V

L

A

S

A

L

G

T

E

R

V

A

L

V

P

F

Y

Y

I

I

A

V

A

M

Q

Q

V

C

A

L

G

G

A

A

I

I

G

C

A

G

S

A

A

G

A

-

L

-

Y

-

L

-

I

-

A

-

N

-

G

-

Q

-

P

-

D

-

F

-

A

V

I

V

G

K

G

G

F

F

A

Q

A

P

N

N

G

P

Y

Y

G

Q

P

T

L

L

S

G

P

G

G

G

L

A

-

N

-

T

-

V

-

A

-

H

-

G

F

Y

D

T

M

K

K

G

A

S

A

G

L

L

L

G

A

A

E

E

C

I

I

I

A

G

T

T

F

F

F

V

F

L

L

V

F

Y

I

T

I

V

M

F

R

S

V

A

T

T

S

D

-

A

-

K

-

R

-

S

-

A

-

R

S

D

G

S

A

H

V

V

P

P

G

I

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

L

I

V

H

H

L

L

V

A

L

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

L

I

F

I

A

Y

G

N

G

K

E

D

Y

H

-

A

L

W

A

D

Q

D

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

G

A

V

A

M

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 85% coverage: 7:219/251 of query aligns to 56:271/286 of Q06611

query
sites
Q06611

A

A

E

E

F

F

I

I

G

A

T

T

F

F

W

L

L

F

T

L

F

Y

G

I

G

T

C

V

G

L

S

T

A

V

I

M

L

G

A

V

A

K

A

R

F

S

P

P

E

N

L

M

-

C

-

A

G

S

I

V

G

G

F

I

V

Q

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

W

F

A

L

G

A

R

R

R

K

V

L

A

S

A

L

G

T

E

R

V

A

L

V

P

Y

Y

Y

I

I

A

V

A

M

Q

Q

V

C

A

L

G

G

A

A

I

I

G

C

A

G

S

A

A

G

A

V

L

V

Y

K

L

G

I

F

A

Q

N

P

G

K

Q

Q

P

Y

D

Q

F

-

A

A

I

L

G

G

F

-

A

-

N

-

G

G

Y

A

G

N

P

T

L

I

S

A

P

H

G

G

L

-

F

Y

D

T

M

K

K

G

A

S

A

G

L

L

L

G

A

A

E

E

C

I

I

I

A

G

T

T

F

F

F

V

F

L

L

V

F

Y

I

T

I

V

M

F

R

S

V

A

T

T

S

D

-

A

-

K

-

R

-

N

-

A

-

R

S

D

G

S

A

H

V

V

P

P

G

I

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

L

I

V

H

H

L

L

V

A

L

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

P

A

A

A

-

I

L

I

F

F

A

N

G

K

G

D

E

N

Y

A

L

W

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

G

A

V

A

M

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 75% coverage: 33:219/251 of query aligns to 75:262/285 of P30302

query
sites
P30302

G

G

I

V

G

G

F

I

V

L

G

G

V

I

S

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

G

G

L

L

W

F

A

L

G

A

R

R

R

K

V

V

A

S

A

L

G

I

E

R

V

A

L

V

P

L

Y

Y

I

M

A

V

A

A

Q

Q

V

C

A

L

G

G

A

A

I

I

-

C

G

G

A

V

S

G

A

F

A

V

L

K

Y

A

L

F

I

Q

A

S

N

S

G

H

Q

Y

P

V

D

N

F

Y

A

G

I

G

G

G

-

A

G

N

F

F

A

L

A

A

N

D

G

G

Y

Y

G

-

P

-

L

-

S

-

P

-

G

-

L

-

F

-

D

N

M

T

K

G

A

T

A

G

L

L

L

A

A

A

E

E

C

I

I

I

A

G

T

T

F

F

F

V

F

L

L

V

F

Y

I

T

I

V

M

F

R

S

V

A

T

T

S

D

-

P

-

K

-

R

-

N

-

A

-

R

S

D

G

S

A

H

V

V

P

P

G

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

L

F

A

A

L

V

T

F

L

M

I

V

H

H

L

L

V

A

L

T

I

I

P

P

V

I

T

T

N

G

T
|
T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

A

A

A

-

V

L

I

F

F

A

N

G

K

G

S

E

K

Y

P

L

W

A

D

Q

D

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

G

A

V

T

M

I

G

A

A

A

L

F

T223 (= T181) mutation to C: Creates some mercury-sensitivity.

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
32% identity, 96% coverage: 7:247/251 of query aligns to 15:253/271 of P41181

query
sites
P41181

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

L

L

F

T

V

F

F

G

F

G

G

C

L

G

G

S

S

A

A

I

L

L

-

A

-

A

N

F

W

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S

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x
A

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x
V

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-

Y

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L

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G64 (= G59) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (= G73) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ R74) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
S148 (≠ V150) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R187) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G190) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ F194) to I: in dbSNP:rs772051028
S216 (≠ A218) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
L217 (= L219) mutation to A: Abolishes interaction with MIAC; when associated with A-221.
Y221 (vs. gap) mutation to A: Abolishes interaction with MIAC; when associated with A-217.
S229 (= S223) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
S231 (≠ G225) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
E232 (= E226) mutation to A: Reduces interaction with MIAC.
T244 (≠ P238) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.

Sites not aligning to the query:

254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
35% identity, 75% coverage: 33:219/251 of query aligns to 75:262/285 of P43287

query
sites
P43287

G

G

I

V

G

G

F

I

V

L

G

G

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I

S

A

L

W

A

A

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Y

A

C

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A

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N

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A

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R194 (vs. gap) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
D195 (≠ S153) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
H197 (≠ A155) mutation to A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; mutation to D: Reduced water transport activity. Insensitive to cytosolic acidification.; mutation to K: Very low water transport activity. Insensitive to cytosolic acidification.

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial
264 H→A: No effect.

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 2 papers)
35% identity, 98% coverage: 3:248/251 of query aligns to 12:257/265 of P55064

query
sites
P55064

K

K

R

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A

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A38 (≠ L32) to E: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123054
I45 (≠ V39) to S: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123055
N123 (≠ G120) to D: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123057
S156 (≠ P157) mutation to A: No effect on location at the cell membrane.; mutation to E: Increased location at the cell membrane.
I177 (= I176) to F: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123056
R188 (= R187) to C: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs368292687

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 75% coverage: 33:219/251 of query aligns to 77:264/287 of P43286

query
sites
P43286

G

G

I

V

G

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F

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G

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A

A

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L

F

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

Q39196 Probable aquaporin PIP1-4; Plasma membrane intrinsic protein 1-4; AtPIP1;4; Transmembrane protein C; TMP-C from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 85% coverage: 7:219/251 of query aligns to 57:272/287 of Q39196

query
sites
Q39196

A

A

E

E

F

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A

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A

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I

G

G

G

A

V

A

M

L

G

A

A

A

L

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
36% identity, 96% coverage: 3:244/251 of query aligns to 11:252/253 of 5dyeD

query
sites
5dyeD

K

K

R

A

L

V

T

F

A

A

E

E

F

F

I

L

G

A

T

T

F

L

W

I

L

F

T

V

F

F

G

F

G

G

C

L

G

G

S

S

A

A

I

L

L

-

A

-

A

K

F

W

P

P

E

S

L

A

G

L

I

P

G

T

F

I

V

L

G

Q

V

I

S

A

L

L

A

A

F

F

G

G

L

L

T

A

V

I

L

G

T

T

M

L

A

A

Y

Q

A

A

V

L

G

G

G

P

I

V

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

I

T

T

L

L

G

A

L

L

W

L

A

V

G

G

R

N

R

Q

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I

A

S

A

L

G

L

E

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V

A

L

F

P

F

Y

Y

I

V

A

A

Q

Q

V

L

A

V

G

G

A

A

I

I

G

A

A

G

S

A

A

G

A

I

L

L

Y

Y

L

G

I

V

A

A

N

-

G

-

Q

-

P

P

D

L

F

N

A

A

I

R

G

G

G

N

F

L

A

A

A

V

N

N

G

A

Y

L

G

N

P

N

L

N

S

T

P

-

G

-

L

-

F

-

D

T

M

Q

K

G

A

Q

A

A

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M

L

V

A

V

E

E

C

L

I

I

A

L

T

T

F

F

F

Q

F

L

L

A

F

L

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C

I

I

M

F

R

A

V

S

T

T

S

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S

S

G

R

A

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E

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P

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G
|
G

F
x
S

A

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A

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L

A

S

I

I

G

G

L

L

A

S

L

V

T

T

L

L

I

G

H

H

L

L

V

V

L

G

I

I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

P

A

A

L

V

F

V

A

M

G

-

G

N

E

R

Y

F

L

S

A

P

Q

A

L

H

W

W

L

V

F

F

W

W

L

V

A

G

P

P

M

I

V

V

G

G

G

A

V

V

M

L

G

A

A

A

L

I

-

L

-

Y

-

F

-

Y

-

L

-

F

-

P

A

N

A

S

R

L

S

S

L

L

G

S

E

E

R

R

D

V

K

A

-

I

S

I

A

K

G

G

P

T

P

Y

Q

E

P

P

A

D

P

E

P

W

C

E

D

E

Q

Q

R

R

R

E

binding O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine: G158 (= G158), S159 (≠ F159)

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
35% identity, 86% coverage: 3:219/251 of query aligns to 10:223/247 of 8ct2D

query
sites
8ct2D

K

R

R

A

L

V

T

V

A

A

E

E

F

F

I

L

G

A

T

T

F

T

W

L

L

F

T

V

F

F

G

I

G

S

C

I

G

G

S

S

A

A

I

-

L

L

A

G

A

F

A

K

F

Y

P

P

-

V

-

G

-

N

E

Q

L

T

G

A

I

V

-

Q

G

D

F

N

V

V

G

K

V

V

S

S

L

L

A

A

F

F

G

G

L

L

T

S

V

I

L

A

T

T

M

L

A

A

Y

Q

A

S

V

V

G

G

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H

I

I

S

S

G

G

G

A

H

H

F

L

N

N

P

P

A

A

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T

L

L

G

G

L

L

W

L

A

L

G

S

R

C

R

Q

V

I

A

S

A

I

G

F

E

R

V

A

L

L

P

M

Y

Y

I

I

A

I

A

A

Q

Q

V

C

A

V

G

G

A

A

I

I

G

V

A

A

S

T

A

A

A

I

L

L

Y

S

L

G

I

I

A

T

N

S

-

S

-

L

-

T

-

G

-

N

-

S

-

L

G

G

Q

R

P

N

D

D

F

L

A

A

I

D

G

G

G

V

F

N

A

S

A
x
G

N

Q

G

G

Y

L

G
|
G

P

-

L

-

S

-

P

-

G

-

L

-

F

-

D

-

M

-

K

-

A

-

L

-

A

I

E

E

C

I

I

I

A

G

T

T

F

L

F

Q

F

L

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F

L

I

C

I

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M

L

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A

V

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T

T

S

D

-

R

S

R

G

R

A

D

V

L

P

G

G

G

F

S

A

A

P

P

I

L

A

A

I

I

G

G

L

L

A

S

L

V

T

A

L

L

I

G

H

H

L

L

V

L

L

A

I

I

P

D

V

Y

T

T

N

G

T

C

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

P

S

A

A

L

V

F

I

A

T

G

-

E

H

Y

N

L

F

A

S

Q

N

L

H

W

W

L

I

F

F

W

W

L

V

A

G

P

P

M

F

V

I

G

G

V

A

M

L

G

A

A

V

L

L

binding cholesterol: G134 (≠ A116), G138 (= G120)

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
31% identity, 98% coverage: 3:247/251 of query aligns to 11:253/271 of P56402

query
sites
P56402

K

R

R

A

L

V

T

L

A

A

E

E

F

F

I

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G

A

T

T

F

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W

L

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F

T

V

F

F

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F

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C

L

G

G

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A

A

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L

Q

A

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A

A

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S

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P

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L

-

G

-

I

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G

S

F

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A

L

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A

A

F

F

G

G

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L

T

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I

L

G

T

T

M

L

A

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Y

Q

A

A

V

L

G

G

G

H

I

V

S

S

G

G

G

A

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

V

G

A

L

C

W

L

A

V

G

G

R

C

R

H

V

V

A

S

A

F

G

L

E

R

V

A

L

A

P

F

Y

Y

I

V

A

A

Q

Q

V

L

A

L

G

G

A

A

I

V

G

A

A

G

S

A

A

A

A

I

L

L

Y

H

L

E

I

I

A

T

-

P

-

V

N

E

G

I

Q

R

P

G

D

D

F

L

A

A

I

V

G

N

G

A

F

L

A

H

A

N

N

N

G

A

Y
x
T

G

A

P

-

L

-

S

-

P

-

G

-

L

-

F

-

D

-

M

-

K

G

A

Q

A

A

L

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L

T

A

V

E

E

C

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F

A

L

T

T

F

M

F

Q

F

L

L

V

F

L

I

C

I

I

M

F

R

A

V

S

T

T

S

D

S

E

G

R

A

R

V

S

P

D

G

N

F

L

A

G

P

S

-

P

-

A

I

L

A

S

I

I

G

G

L

F

A

S

L

V

T

T

L

L

I

G

H

H

L

L

V

L

L

G

I

I

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Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

A

P

P

A

A

L

V

F

V

A

T

G

G

G

K

E

-

Y

-

L

F

A

D

Q

D

L

H

W

W

L

V

F

F

W

W

L

I

A

G

P

P

M

L

V

V

G

G

G

A

V

V

M

I

G

G

A

S

L

L

-

L

-

Y

-

N

-

Y

-

L

-

F

-

P

A

S

A

T

R

K

S

S

L

L

G

Q

E

E

R

R

D

L

K

A

S

V

A

L

G

K

P

G

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Q

E

P

P

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P

D

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W

D

E

Q

E

R

R

R

E

V

V

G

R

R

R

T126 (≠ Y119) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

Query Sequence

>GFF3160 FitnessBrowser__WCS417:GFF3160
MQKRLTAEFIGTFWLTFGGCGSAILAAAFPELGIGFVGVSLAFGLTVLTMAYAVGGISGG
HFNPAVTLGLWAGRRVAAGEVLPYIAAQVAGAIGASAALYLIANGQPDFAIGGFAANGYG
PLSPGLFDMKAALLAECIATFFFLFIIMRVTSSGAVPGFAPIAIGLALTLIHLVLIPVTN
TSVNPARSTGPALFAGGEYLAQLWLFWLAPMVGGVMGALAARSLGERDKSAGPPQPAPPC
DQRRVGRSTPC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory