SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3252 FitnessBrowser__Marino:GFF3252 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
40% identity, 88% coverage: 14:134/138 of query aligns to 6:125/204 of 7bgnA

query
sites
7bgnA

L

L

D

D

A

S

I

V

R

K

W

W

T

D

E

N

D

K

G

G

L

L

V

A

P

V

A

A

I

I

A

A

Q

Q

D

N

A

V

D

D

T

T

G

G

D

A

I

I

L

L

M

M

M

Q

A

G

W

F

M

A

N

N

R

R

E

E

S

A

L

V

R

A

L

T

T

T

A

I

E

S

E

S

G

R

H

K

A

A

V

T

Y

F

W

Y

S

S

R

R

S

S

R

R

G

S

K

S

L

L

W
|
W

R

T

K
|
K

G
|
G

E

E

T
|
T

S
|
S

G

N

H

N

Q

F

Q

I

V

N

I

V

R

H

D

D

I

V

R

F

L

L

D
|
D

C

C

D
|
D

E

R

D
|
D

V

S

I

I

L

I

L

Y

K

-

V

L

E

G

Q

K

K

P

G

D

G

G

I

P

A
x
T

C
|
C

H
|
H

T

T

G

G

R

A

R

E

S

T

C
|
C

F

Y

Y

Y

R

T

T

P

L

V

K

F

D

D

G

L

Q

N

W

K

V

L

S

A

V

L

D

T

P

S

V

L

I

Y

K

A

D
x
L

P

E

G

S

T

T

I

I

binding adenosine monophosphate: W60 (= W68), K62 (= K70), G63 (= G71), T65 (= T73), S66 (= S74), T94 (≠ A103), H96 (= H105), L121 (≠ D130)
binding zinc ion: D78 (= D86), D80 (= D88), D82 (= D90), C95 (= C104), C102 (= C111)

Sites not aligning to the query:

binding adenosine monophosphate: 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 76% coverage: 14:118/138 of query aligns to 8:111/213 of 7bgmA

query
sites
7bgmA

L

L

D

D

A

S

I

V

R

K

W

W

T

D

E

N

D

K

G

G

L

L

V

A

P

V

A

A

I

I

A

A

Q

Q

D

N

A

V

D

D

T

T

G

G

D

A

I

I

L

L

M

M

M

Q

A

G

W

F

M

A

N

N

R

R

E

E

S

A

L

V

R

A

L

T

T

T

A

I

E

S

E

S

G

R

H

K

A

A

V

T

Y

F

W

Y

S

S

R

R

S

S

R

R

G

S

K

S

L

L

W

W

R

T

K

K

G

G

E

E

T

T

S

S

G

N

H

N

Q

F

Q

I

V

N

I

V

R

H

D

D

I

V

R

F

L

L

D
|
D

C

C

D
|
D

E

R

D
|
D

V

S

I

I

L

I

L

Y

K

-

V

L

E

G

Q

K

K

P

G

D

G

G

I

P

A

T

C
|
C

H

H

T

T

G

G

R

A

R

E

S

T

C
|
C

F

Y

Y

Y

R

T

T

P

L

V

K

F

D

D

binding zinc ion: D80 (= D86), D82 (= D88), D84 (= D90), C97 (= C104), C104 (= C111)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lA Crystal structure of bi-functional enzyme (see paper)
43% identity, 73% coverage: 22:122/138 of query aligns to 15:112/200 of 6j2lA

query
sites
6j2lA

D

D

G

G

L

L

V

M

P

P

A

V

I

I

A

V

Q

Q

D

H

A

A

D

V

T

S

G

G

D

E

I

V

L

L

M

M

M

L

A

G

W

Y

M

M

N

N

R

P

E

E

S

A

L

L

R

D

L

K

T

T

A

I

E

E

E

S

G

G

H

K

A

V

V

T

Y

F

W

F

S

S

R

R

S

T

R

K

G

Q

K

R

L

L

W

W

R

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

Q

F

Q

L

V

N

I

V

R

V

D

S

I

I

R

A

L

P

D
|
D

C

C

D
|
D

E

N

D
|
D

V

T

I

L

L

L

L

V

K

-

V

L

E

A

Q

N

K

P

G

I

G

G

I

P

A

T

C
|
C

H

H

T

K

G

G

R

T

R

S

S

S

C
|
C

F

F

Y

G

R

N

T

T

L

A

K

H

D

-

G

-

Q

Q

W

W

V

L

binding zinc ion: D79 (= D86), D81 (= D88), D83 (= D90), C96 (= C104), C103 (= C111)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
41% identity, 73% coverage: 22:122/138 of query aligns to 14:105/185 of 6j2lB

query
sites
6j2lB

D

D

G

G

L

L

V

M

P

P

A

V

I

I

A

V

Q

Q

D

H

A

A

D

V

T

S

G

G

D

E

I

V

L

L

M

M

M

L

A

G

W

Y

M

M

N

N

R

P

E

E

S

A

L

L

R

D

L

K

T

T

A

I

E

E

E

S

G

G

H

K

A

V

V

T

Y

F

W

F

S

S

R

R

S

T

R

K

G

Q

K

R

L

L

W

W

R

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

Q

F

Q

L

V

N

I

V

R

V

D

S

I

I

R

A

L

P

D
|
D

C

C

D
|
D

E

N

D
|
D

V

T

I

L

L

L

L

V

K

-

V

-

E

-

Q

-

K

-

G

-

G

-

I

L

A

A

C

N

H

P

T

I

G

G

R

P

R

S

S

S

C

C

F

F

Y

G

R

N

T

T

L

A

K

H

D

-

G

-

Q

Q

W

W

V

L

binding zinc ion: D78 (= D86), D80 (= D88), D82 (= D90)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>GFF3252 FitnessBrowser__Marino:GFF3252
MQNSSDNVETPDWLDAIRWTEDGLVPAIAQDADTGDILMMAWMNRESLRLTAEEGHAVYW
SRSRGKLWRKGETSGHQQVIRDIRLDCDEDVILLKVEQKGGIACHTGRRSCFYRTLKDGQ
WVSVDPVIKDPGTIYGSN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory