SitesBLAST – Find functional sites

 

SitesBLAST

Comparing GFF3252 FitnessBrowser__psRCH2:GFF3252 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
23% identity, 97% coverage: 3:151/154 of query aligns to 501:650/650 of O31645

query
sites
O31645
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E

Sites not aligning to the query:

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
27% identity, 81% coverage: 27:151/154 of query aligns to 19:143/143 of C0H3V2

query
sites
C0H3V2
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E
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N

P46321 Probable licABCH operon regulator; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
24% identity, 80% coverage: 24:146/154 of query aligns to 513:634/641 of P46321

query
sites
P46321
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V

Sites not aligning to the query:

Query Sequence

>GFF3252 FitnessBrowser__psRCH2:GFF3252
MIRLENILTPGRSLVNVPGGSKKRVLEQIAKVIAQDLIDLDSQTIFESLIAREKLGSTGF
GNGIAIPHCRMVGCSAPLSAVLRLETPVDFDAIDGAPVDLLFVLLVPQAATDEHLELLRQ
IASMLDREDVRQRLREAPTNQSLYQVVVDVQNGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory