SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3260 FitnessBrowser__Phaeo:GFF3260 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
42% identity, 100% coverage: 4:815/816 of query aligns to 22:823/824 of Q8GAI3

query
sites
Q8GAI3

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W66 (= W48) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (= H49) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
33% identity, 99% coverage: 6:816/816 of query aligns to 4:815/824 of 4pabB

query
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4pabB

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active site: T53 (≠ A55), E102 (= E104), H226 (= H234), Y255 (= Y259), E536 (≠ D538)
binding flavin-adenine dinucleotide: I11 (= I13), G12 (= G14), G13 (= G15), G14 (= G16), C15 (≠ V17), V16 (≠ I18), L35 (= L37), E36 (= E38), K37 (≠ R39), A42 (≠ S44), G43 (= G45), S44 (≠ T46), T45 (= T47), H47 (= H49), A48 (= A50), A49 (= A51), G50 (= G52), L51 (= L53), P174 (≠ K176), V175 (= V177), A203 (≠ C211), A204 (= A212), G205 (= G213), F206 (≠ M214), W207 (= W215), H226 (= H234), Y228 (= Y236), V324 (≠ F323), G326 (= G325), P327 (= P326), I328 (≠ E327), F353 (= F352), G354 (≠ N353), Y355 (≠ I355), G356 (= G356), I357 (= I357), I358 (≠ Q358)
binding (6s)-5,6,7,8-tetrahydrofolate: F493 (= F494), I523 (≠ Y525), E536 (≠ D538), T538 (= T540), I550 (≠ V552), F612 (= F612), L613 (≠ G613), I630 (≠ V630), Y632 (= Y632), E639 (= E639), Y641 (= Y641), F680 (= F680), Y700 (≠ F700), F701 (≠ A701)

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
33% identity, 99% coverage: 6:816/816 of query aligns to 41:852/857 of Q63342

query
sites
Q63342

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CV 52:53 (≠ VI 17:18) binding
EK 73:74 (≠ ER 38:39) binding
80:88 (vs. 45:53, 89% identical) binding
H84 (= H49) modified: Tele-8alpha-FAD histidine
V212 (= V177) binding
W244 (= W215) binding
FG-YGII 390:395 (≠ FNSIGIQ 352:358) binding
ELT 573:575 (≠ DVT 538:540) binding
Y669 (= Y632) binding
ELY 676:678 (≠ EIY 639:641) binding
Y737 (≠ F700) binding

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
34% identity, 99% coverage: 6:816/816 of query aligns to 48:859/866 of Q9UI17

query
sites
Q9UI17

N

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x
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K

K

Y

V

V

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G

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Y

N

L

T

S

T

S

S

G

G

N

S

Y

Y

G

S

H

Y

T

S

L

I

A

Q

A

K

A

S

I

L

G

A

M

F

G

A

Y

Y

V

V

P

P

C

V

E

Q

G

-

E

-

S

-

A

-

A

L

D

S

V

E

L

V

G

G

S

Q

S

Q

Y

V

E

E

I

V

D

E

V

L

C

L

G

G

V

K

K

N

V

Y

K

P

A

A

E

V

V

I

S

I

L

Q

K

E

P

P

M

L

Y

V

-

L

-

T

D

E

P

P

K

T

S

R

E

N

R

R

V

L

K

Q

Q

K

CV 59:60 (≠ VI 17:18) binding
EK 80:81 (≠ ER 38:39) binding
87:95 (vs. 45:53, 89% identical) binding
H91 (= H49) modified: Tele-8alpha-FAD histidine
H109 (≠ A67) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (= V177) binding
S279 (≠ T243) to P: in dbSNP:rs532964
FG-YGII 397:402 (≠ FNSIGIQ 352:358) binding
A530 (≠ G487) to G: in dbSNP:rs1805073
S646 (≠ P602) to P: in dbSNP:rs1805074

1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
33% identity, 99% coverage: 9:815/816 of query aligns to 3:826/827 of 1pj7A

query
sites
1pj7A

R

R

V

I

V

V

I

I

I
|
I

G
|
G

G

A

G
|
G

V
x
I

I
x
V

G

G

C

T

S

N

V

L

A

A

Y

D

H

E

L

L

T

V

K

T

L

R

G

G

W

W

K

N

D

N

V

I

V

T

L

V

L

L

E
x
D

R
x
Q

K

G

Q

P

L

L

T

N

-

M

-
x
P

S

G

G
|
G

T
x
S

T
|
T

W

S

H
|
H

A
|
A

A
x
P

G
|
G

L
|
L

I

V

A

F

Q

Q

L

T

R

N

A

P

T

S

A

K

N

T

M

M

T

A

K

S

L

F

A

A

K

K

Y

Y

S

T

Q

V

E

E

L

K

Y

L

G

L

A

S

L

L

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E

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T

-

G

G

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V

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C

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F

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N

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G

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V

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K

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Y

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Y

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G

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|
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G

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D

M

I

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V

V

V

N

S

C
|
C

A
|
A

G
|
G

M

F

W
|
W

G

G

H

A

E

K

V
x
I

G

G

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A

M

M

A

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G

G

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M

N

A

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P

L

L

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L

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C

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|
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|
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|
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W

A

K

V

T

R

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T

N

A

I

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G

A

M

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Y

Y

D

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M

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L

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Y

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D

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T

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S

E

E

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D

A

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Y

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-
x
G

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x
A

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active site: H222 (= H234), Y256 (= Y259), D549 (= D538)
binding flavin-adenine dinucleotide: I7 (= I13), G8 (= G14), G10 (= G16), I11 (≠ V17), V12 (≠ I18), D32 (≠ E38), Q33 (≠ R39), P39 (vs. gap), G41 (= G45), S42 (≠ T46), T43 (= T47), H45 (= H49), A46 (= A50), P47 (≠ A51), G48 (= G52), L49 (= L53), T169 (≠ I175), T170 (≠ K176), V171 (= V177), C199 (= C211), A200 (= A212), G201 (= G213), W203 (= W215), I207 (≠ V219), H222 (= H234), Y256 (= Y259), G330 (= G325), I331 (≠ P326), F332 (≠ E327), V357 (≠ I355), W358 (≠ G356), V359 (≠ I357), T360 (≠ Q358)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: M502 (= M491), L505 (≠ F494), Y536 (= Y525), D549 (= D538), T551 (= T540), G563 (vs. gap), A564 (vs. gap), N565 (vs. gap), Y628 (≠ F612), F629 (≠ G613), L646 (≠ V630), Y648 (= Y632), E655 (= E639), Y657 (= Y641), Y696 (≠ F680), R697 (= R681), F716 (= F700)

1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
33% identity, 99% coverage: 9:815/816 of query aligns to 4:827/828 of 1pj6A

query
sites
1pj6A

R

R

V

I

V

V

I

I

I
|
I

G
|
G

G

A

G
|
G

V
x
I

I
x
V

G

G

C

T

S

N

V

L

A

A

Y

D

H

E

L

L

T

V

K

T

L

R

G

G

W

W

K

N

D

N

V

I

V

T

L

V

L

L

E
x
D

R
x
Q

K

G

Q

P

L

L

T

N

-

M

-
x
P

S

G

G
|
G

T
x
S

T
|
T

W

S

H
|
H

A

A

A
x
P

G
|
G

L
|
L

I

V

A

F

Q

Q

L

T

R

N

A

P

T

S

A

K

N

T

M

M

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A

K

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L

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A

A

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K

Y

Y

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V

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E

L

K

Y

L

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L

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L

L

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-

G

G

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V

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F

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G

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D

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C

A
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A

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M

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W
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W

G

G

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A

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x
I

G

G

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M

A

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A

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P

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L

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L

A

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C

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A

H
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H

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Q

Y

Y

I

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Y

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