SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3277 FitnessBrowser__WCS417:GFF3277 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
47% identity, 96% coverage: 10:253/255 of query aligns to 3:238/240 of 1ji0A

query
sites
1ji0A

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binding adenosine-5'-triphosphate: Y16 (= Y23), G42 (= G50), G44 (= G52), K45 (= K53), T46 (≠ S54), T47 (= T55), E88 (= E101), E165 (= E179)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 91% coverage: 21:253/255 of query aligns to 11:253/253 of 1g9xB

query
sites
1g9xB

V

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binding adenosine-5'-diphosphate: F14 (≠ D24), F17 (≠ I27), G40 (= G50), S41 (≠ A51), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55)
binding magnesium ion: S44 (= S54), E176 (= E179)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 92% coverage: 21:254/255 of query aligns to 11:254/254 of 1g6hA

query
sites
1g6hA

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binding adenosine-5'-diphosphate: F14 (≠ D24), F17 (≠ I27), N39 (= N49), G40 (= G50), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
33% identity, 89% coverage: 30:255/255 of query aligns to 18:237/240 of 6mjpA

query
sites
6mjpA

V

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Q

binding calcium ion: E111 (≠ Q127), D115 (≠ H131)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
32% identity, 95% coverage: 12:254/255 of query aligns to 1:236/238 of 6s8nB

query
sites
6s8nB

A

A

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x
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G

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E

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ G107), M133 (≠ R150), G134 (≠ A151), Q135 (≠ G152)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
32% identity, 95% coverage: 12:254/255 of query aligns to 1:236/238 of 6s8gA

query
sites
6s8gA

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A
x
R

I

R

L

V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P

L

S

H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L

P

E
x
Q

G

E

R

A

H

S

V

I

F

F

G

R

Q

R

L

L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G

V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G

Q

L
x
S

T

L

S
|
S

G

G

G

G

E
|
E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R

R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E

E

P

P

S

F

M

A

G

G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q

H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

L

L

G

G

K

E

binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y23), R15 (≠ A26), N37 (= N49), G40 (= G52), K41 (= K53), T42 (≠ S54), T43 (= T55), Q84 (≠ E101), S136 (≠ L153), S138 (= S155), E141 (= E158)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
32% identity, 95% coverage: 12:253/255 of query aligns to 1:235/235 of 6mhzA

query
sites
6mhzA

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A

R

I

R

L

V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P

L

S

H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L

P

E
x
Q

G

E

R

A

H

S

V

I

F

F

G

R

Q

R

L

L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G

V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G

Q

L
x
S

T

L

S
|
S

G
|
G

G
|
G

E

E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R

R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E
|
E

P

P

S

F

M

A

G
|
G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q
x
H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

L

L

G

G

binding adp orthovanadate: Y12 (= Y23), N37 (= N49), G38 (= G50), G40 (= G52), K41 (= K53), T42 (≠ S54), T43 (= T55), Q84 (≠ E101), S136 (≠ L153), S138 (= S155), G139 (= G156), G140 (= G157), E162 (= E179), G166 (= G183), H194 (≠ Q212)

6mbnA Lptb e163q in complex with atp (see paper)
32% identity, 95% coverage: 12:254/255 of query aligns to 2:237/241 of 6mbnA

query
sites
6mbnA

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A
x
R

I

R

L
x
V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P

L

S

H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L

P

E

Q

G

E

R

A

H

S

V

I

F

F

G

R

Q

R

L

L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G

V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G

Q

L

S

T

L

S

S

G

G

G

G

E

E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R

R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E

Q

P

P

S

F

M

A

G

G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q
x
H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

L

L

G

G

K

E

binding adenosine-5'-triphosphate: Y13 (= Y23), R16 (≠ A26), V18 (≠ L28), N38 (= N49), G39 (= G50), G41 (= G52), K42 (= K53), T43 (≠ S54), T44 (= T55), H195 (≠ Q212)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
32% identity, 95% coverage: 12:252/255 of query aligns to 1:234/234 of 6b89A

query
sites
6b89A

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A
x
R

I

R

L
x
V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P
x
L

S
x
H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L
x
P

E
x
Q

G

E

R
x
A

H
x
S

V

I

F
|
F

G
x
R

Q
x
R

L
|
L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G
x
V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G
x
Q

L

S

T

L

S

S

G

G

G

G

E

E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R
|
R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E

E

P

P

S

F

M

A

G

G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q

H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

L

L

binding adenosine-5'-diphosphate: Y12 (= Y23), R15 (≠ A26), V17 (≠ L28), N37 (= N49), G38 (= G50), G40 (= G52), K41 (= K53), T42 (≠ S54), T43 (= T55)
binding magnesium ion: T42 (≠ S54), Q84 (≠ E101)
binding novobiocin: L71 (≠ P88), H72 (≠ S89), P83 (≠ L100), A86 (≠ R103), S87 (≠ H104), F89 (= F106), R90 (≠ G107), R91 (≠ Q108), L92 (= L109), V101 (≠ G118), Q135 (≠ G152), R149 (= R166)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
32% identity, 95% coverage: 12:252/255 of query aligns to 1:234/234 of 4p31A

query
sites
4p31A

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A
x
R

I

R

L
x
V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P

L

S

H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L

P

E
x
Q

G

E

R

A

H

S

V

I

F

F

G

R

Q

R

L

L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G

V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G

Q

L

S

T

L

S

S

G

G

G

G

E

E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R

R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E

E

P

P

S

F

M

A

G

G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q

H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

L

L

binding adenosine-5'-diphosphate: Y12 (= Y23), R15 (≠ A26), V17 (≠ L28), G38 (= G50), G40 (= G52), K41 (= K53), T42 (≠ S54), T43 (= T55)
binding magnesium ion: T42 (≠ S54), Q84 (≠ E101)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
32% identity, 94% coverage: 12:251/255 of query aligns to 1:233/233 of 6b8bA

query
sites
6b8bA

A

A

L

T

L

L

S

T

V

A

N

K

D

N

I

L

E

A

V

K

I

A

Y
|
Y

D

K

G

G

A
x
R

I

R

L
x
V

A

-

V

V

A

E

G

D

V

V

S

S

L

L

S

T

V

V

P

N

K

S

G

G

A

E

T

I

V

V

A

G

L

L

L

L

G

G

A

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

F

K

Y

A

M

I

V

S

V

G

G

L

I

V

V

R

P

A

R

E

D

R

A

A

-

E

-

V

-

S

-

R

-

G

G

T

N

I

I

E

I

Y

I

A

D

G

D

T

D

D

D

L

I

A

S

G

L

I

L

D

P

P

L

S

H

Q

A

R

R

V

A

R

R

Q

R

G

G

M

I

V

G

H

Y

V

L

L

P

E

Q

G

E

R

A

H

S

V

I

F
|
F

G
x
R

Q
x
R

L

L

S

S

V

V

E

Y

D

D

N

N

L

L

R

M

S

A

G

V

G

L

F

Q

V

I

R

R

R

D

-

D

L

L

S

S

R

A

Q

E

E

Q

M

R

E

E

H

D

D

R

L

A

E

N

R

E

L

L

Y

M

A

E

W

E

F

F

P

-

R

H

L

I

K

E

T

H

K

L

R

R

H

D

T

S

R

M

A

G

G

Q

L

S

T

L

S

S

G

G

G

G

E

E

Q

R

Q

R

M

R

V

V

A

E

I

I

G

A

R

R

A

A

L

L

M

A

T

A

R

N

P

P

T

K

L

F

V

I

L

L

L

L

D

D

E

E

P

P

S

F

M

A

G

G

L

V

A

D

P

P

M

I

I

S

V

V

Q

I

E

D

I

I

F

K

E

R

I

I

I

I

G

E

Q

H

L

L

N

-

R

R

E

D

Q

S

Q

G

V

L

S

G

F

V

L

L

I

I

A

T

E

D

Q

H

N

N

I

V

N

R

V

E

A

T

L

L

N

A

Y

V

A

C

S

E

H

R

G

A

Y

Y

V

I

L

V

D

S

T

Q

G

G

R

H

V

L

A

I

L

A

S

H

G

G

S

T

A

P

A

T

E

E

L

I

L

L

A

Q

R

D

G

E

D

H

L

V

H

K

D

R

I

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y23), R15 (≠ A26), V17 (≠ L28), G38 (= G50), G40 (= G52), K41 (= K53), T42 (≠ S54), T43 (= T55)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F106), R90 (≠ G107), R91 (≠ Q108)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
28% identity, 91% coverage: 13:245/255 of query aligns to 1:227/240 of 4ymuJ

query
sites
4ymuJ

L

M

L

I

S

F

V

V

N

N

D

D

I

V

E

Y

V

K

I

N

Y
x
F

D

-

G

G

A

S

I

L

L

E

A
x
V

V

L

A

K

G

G

V

V

S

T

L

L

S

K

V

V

P

N

K

K

G

G

A

E

T

V

V

V

A

V

L

I

L

I

G

G

A

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

A

C

I

I

S

N

G

L

L

L

V

-

R

-

A

-

E

-

R

-

A

E

E

E

V

P

S

T

R

K

G

G

T

E

I

V

E

F

Y

I

A

D

G

G

T

V

D

K

L

I

A

N

-

N

G

G

I

K

D

V

P

N

S

I

Q

N

R

K

V

V

R

R

Q

Q

G

K

M

V

V

G

H

M

V

V

L

F

E

Q

G

H

R

F

H

N

V

L

F

F

G

P

Q

H

L

L

S

T

V

A

E

I

D

E

N

N

L

I

R

T

S

L

G

A

G

P

F

V

-

K

V

V

R

K

R

K

L

M

S

N

R

K

Q

K

E

E

M

A

E

E

H

E

D

L

L

A

E

V

R

D

L

L

Y

L

A

A

W

K

F

V

P

G

R

L

L

L

K

D

T

K

K

K

R

D

H

Q

T

Y

R

P

A

I

G

K

L

L

T

-

S

S

G

G

G

G

E

Q

Q

K

Q

Q

M

R

V

L

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

M

R

Q

P

P

T

E

L

V

V

M

L

L

L

F

D

D

E
|
E

P

P

S

T

M

S

G

A

L

L

A

D

P

P

M

E

I

M

V

V

Q

K

E

E

I

V

F

L

E

N

I

V

I

M

G

K

Q

Q

L

L

N

A

R

N

E

E

Q

-

Q

G

V

M

S

T

F

M

L

V

I

V

A

V

E

T

Q
x
H

N

E

I

M

N

G

V

F

A

A

L

R

N

E

Y

V

A

G

S

D

H

R

G

V

Y

I

V

F

L

M

D

D

T

D

G

G

R

V

V

I

A

V

L

E

S

E

G

G

S

T

A

P

A

E

E

E

L

I

L

F

A

Y

R

R

G

A

binding adenosine-5'-triphosphate: F11 (≠ Y23), V16 (≠ A29), S36 (≠ N49), G37 (= G50), S38 (≠ A51), G39 (= G52), K40 (= K53), S41 (= S54), T42 (= T55), E162 (= E179), H194 (≠ Q212)
binding magnesium ion: S41 (= S54), E162 (= E179)

P55339 ABC-type transporter ATP-binding protein EcsA from Bacillus subtilis (strain 168) (see paper)
28% identity, 95% coverage: 12:252/255 of query aligns to 2:237/247 of P55339

query
sites
P55339

A

S

L

L

L

L

S

S

V

V

N

K

D

D

I

L

E

T

V

G

I

G

Y

Y

D

T

G

R

A

N

I

P

L

V

A

-

V

L

A

K

G

N

V

V

S

S

L

F

S

T

V

L

P

E

K

P

G

N

A

Q

T

I

V

V

A

G

L

L

L

I

G

G

A

L

N

N

G

G

A

A

G

G

K

K

S

S

T

T

T

T

L

I

K

R

A

H

I

I

S

I

G

G

L

L

V

M

R

-

A

-

E

-

R

-

A

-

E

D

V

P

S

H

R

K

G

G

T

S

I

I

E

E

Y

L

A

N

G

G

T

K

D

T

L

F

A

A

G

E

I

-

D

D

P

P

S

-

Q

E

R

G

V

Y

R

R

Q

S

G

Q

M

F

V

T

H

Y

V

I

L

P

E

E

G

T

R

P

H

V

V

L

F

Y

G

E

Q

E

L

L

S

T

V

L

E

M

D

E

N

H

L

L

R

E

S

L

G

T

G

A

F

M

V

A

R

Y

R

G

L

L

S

S

R

K

Q

E

E

T

M

M

E

E

H

K

D

R

L

L

E

P

R

P

L

L

Y

L

A

K

W

E

F

F

P

-

R

R

L

M

K

E

T

K

K

R

R

L

H

K

T

W

R

F

A

P

G

A

L

H

T

F

S

S

G

K

G

G

E

M

Q

K

Q

Q

M

K

V

V

A

M

I

I

G

M

R

C

A

A

L

F

M

L

T

A

R

E

P

P

T

A

L

L

V

Y

L

I

L

I

D

D

E

E

P

P

S

F

M

L

G
|
G

L

L

A

D

P

P

M

L

I

A

V

I

Q

N

E

A

I

L

F

L

E

E

I

R

I

M

G

N

Q

E

L

-

N

A

R

K

E

K

Q

G

Q

G

V

A

S

S

F

V

L

L

I

M

A

S

E

T

Q

H

N

I

I

L

N

A

V

T

A

A

L

E

N

R

Y

Y

A

C

S

D

H

S

G

F

Y

I

V

I

L

L

D

H

T

N

G

G

R

E

V

V

A

R

L

A

S

R

G

G

S

T

A

L

A

S

E

E

L

L

-

R

-

E

-

Q

-

F

-

G

L

M

A

K

R

D

G

A

D

A

L

L

H

D

D

D

I

L

Y

Y

L

L

G164 (= G183) mutation to E: In ecsA-26; exoamylase and other exoenzymes decrease, competence- and sporulation-deficient phenotype.

3c4jA Abc protein artp in complex with atp-gamma-s
27% identity, 86% coverage: 25:244/255 of query aligns to 14:228/242 of 3c4jA

query
sites
3c4jA

G

G

A

S

I

L

L

E

A
x
V

V

L

A

K

G

G

V

I

S

N

L

V

S

H

V

I

P

R

K

E

G

G

A

E

T

V

V

V

A

V

L

V

L

I

G

G

A

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

F

L

L

K

R

A

C

I

L

S

N

G

L

L

L

V

-

R

-

A

-

E

-

R

-

A

E

E

D

V

F

S

D

R

E

G

G

T

E

I

I

E

I

Y

I

A

D

G

G

T

I

D

N

L

L

A

K

G

A

I

K

D

D

P

T

S

N

-

L

Q

N

R

K

V

V

R

R

Q

E

G

E

M

V

V

G

H

M

V

V

L

F

E

Q

G

R

R

F

H

N

V

L

F

F

G

P

Q

H

L

M

S

T

V

V

E

L

D

N

N

N

L

I

R

T

S

L

G

A

G

P

F

M

-

K

V

V

R

R

R

K

L

W

S

P

R

R

Q

E

E

K

M

A

E

E

H

A

D

K

L

A

E

M

R

E

L

L

Y

L

A

D

W

K

F

V

P

-

R

G

L

L

K

K

T

D

K

K

R

A

H

H

T

A

R

Y

A

P

G

D

L

S

T

L

S

S

G

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

M

R

E

P

P

T

K

L

I

V

M

L

L

L

F

D

D

E

E

P

P

S

T

M

S

G

A

L

L

A

D

P

P

M

E

I

M

V

V

Q

G

E

E

I

V

F

L

E

S

I

V

I

M

G

K

Q

Q

L

L

N

A

R

N

E

E

Q

-

Q

G

V

M

S

T

F

M

L

V

I

V

A

V

E

T

Q

H

N

E

I

M

N

G

V

F

A

A

L

R

N

E

Y

V

A

G

S

D

H

R

G

V

Y

L

V

F

L

M

D

D

T

G

G

G

R

Y

V

I

A

I

L

E

S

E

G

G

S

K

A

P

A

E

E

D

L

L

L

F

A

D

R

R

binding phosphothiophosphoric acid-adenylate ester: V18 (≠ A29), S38 (≠ N49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

3c41J Abc protein artp in complex with amp-pnp/mg2+
27% identity, 86% coverage: 25:244/255 of query aligns to 14:228/242 of 3c41J

query
sites
3c41J

G

G

A

S

I

L

L

E

A
x
V

V

L

A

K

G

G

V

I

S

N

L

V

S

H

V

I

P

R

K

E

G

G

A

E

T

V

V

V

A

V

L

V

L

I

G

G

A

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

T

F

L

L

K

R

A

C

I

L

S

N

G

L

L

L

V

-

R

-

A

-

E

-

R

-

A

E

E

D

V

F

S

D

R

E

G

G

T

E

I

I

E

I

Y

I

A

D

G

G

T

I

D

N

L

L

A

K

G

A

I

K

D

D

P

T

S

N

-

L

Q

N

R

K

V

V

R

R

Q

E

G

E

M

V

V

G

H

M

V

V

L

F

E

Q

G

R

R

F

H

N

V

L

F

F

G

P

Q

H

L

M

S

T

V

V

E

L

D

N

N

N

L

I

R

T

S

L

G

A

G

P

F

M

-

K

V

V

R

R

R

K

L

W

S

P

R

R

Q

E

E

K

M

A

E

E

H

A

D

K

L

A

E

M

R

E

L

L

Y

L

A

D

W

K

F

V

P

-

R

G

L

L

K

K

T

D

K

K

R

A

H

H

T

A

R

Y

A

P

G

D

L

S

T

L

S

S

G

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

M

R

E

P

P

T

K

L

I

V

M

L

L

L

F

D
|
D

E

E

P

P

S

T

M

S

G

A

L

L

A

D

P

P

M

E

I

M

V

V

Q

G

E

E

I

V

F

L

E

S

I

V

I

M

G

K

Q

Q

L

L

N

A

R

N

E

E

Q

-

Q

G

V

M

S

T

F

M

L

V

I

V

A

V

E

T

Q

H

N

E

I

M

N

G

V

F

A

A

L

R

N

E

Y

V

A

G

S

D

H

R

G

V

Y

L

V

F

L

M

D

D

T

G

G

G

R

Y

V

I

A

I

L

E

S

E

G

G

S

K

A

P

A

E

E

D

L

L

L

F

A

D

R

R

binding phosphoaminophosphonic acid-adenylate ester: V18 (≠ A29), S38 (≠ N49), G39 (= G50), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)
binding magnesium ion: S43 (= S54), D163 (= D178)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2olkA Abc protein artp in complex with adp-beta-s
27% identity, 86% coverage: 25:244/255 of query aligns to 14:228/242 of 2olkA

query
sites
2olkA

G

G

A

S

I

L

L

E

A
x
V

V

L

A

K

G

G

V

I

S

N

L

V

S

H

V

I

P

R

K

E

G

G

A

E

T

V

V

V

A

V

L

V

L

I

G

G

A

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

F

L

L

K

R

A

C

I

L

S

N

G

L

L

L

V

-

R

-

A

-

E

-

R

-

A

E

E

D

V

F

S

D

R

E

G

G

T

E

I

I

E

I

Y

I

A

D

G

G

T

I

D

N

L

L

A

K

G

A

I

K

D

D

P

T

S

N

-

L

Q

N

R

K

V

V

R

R

Q

E

G

E

M

V

V

G

H

M

V

V

L

F

E

Q

G

R

R

F

H

N

V

L

F

F

G

P

Q

H

L

M

S

T

V

V

E

L

D

N

N

N

L

I

R

T

S

L

G

A

G

P

F

M

-

K

V

V

R

R

R

K

L

W

S

P

R

R

Q

E

E

K

M

A

E

E

H

A

D

K

L

A

E

M

R

E

L

L

Y

L

A

D

W

K

F

V

P

-

R

G

L

L

K

K

T

D

K

K

R

A

H

H

T

A

R

Y

A

P

G

D

L

S

T

L

S

S

G

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

M

R

E

P

P

T

K

L

I

V

M

L

L

L

F

D

D

E

E

P

P

S

T

M

S

G

A

L

L

A

D

P

P

M

E

I

M

V

V

Q

G

E

E

I

V

F

L

E

S

I

V

I

M

G

K

Q

Q

L

L

N

A

R

N

E

E

Q

-

Q

G

V

M

S

T

F

M

L

V

I

V

A

V

E

T

Q

H

N

E

I

M

N

G

V

F

A

A

L

R

N

E

Y

V

A

G

S

D

H

R

G

V

Y

L

V

F

L

M

D

D

T

G

G

G

R

Y

V

I

A

I

L

E

S

E

G

G

S

K

A

P

A

E

E

D

L

L

L

F

A

D

R

R

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: V18 (≠ A29), S38 (≠ N49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13

2oljA Abc protein artp in complex with adp/mg2+
27% identity, 86% coverage: 25:244/255 of query aligns to 14:228/242 of 2oljA

query
sites
2oljA

G

G

A

S

I

L

L

E

A
x
V

V

L

A

K

G

G

V

I

S

N

L

V

S

H

V

I

P

R

K

E

G

G

A

E

T

V

V

V

A

V

L

V

L

I

G

G

A

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

F

L

L

K

R

A

C

I

L

S

N

G

L

L

L

V

-

R

-

A

-

E

-

R

-

A

E

E

D

V

F

S

D

R

E

G

G

T

E

I

I

E

I

Y

I

A

D

G

G

T

I

D

N

L

L

A

K

G

A

I

K

D

D

P

T

S

N

-

L

Q

N

R

K

V

V

R

R

Q

E

G

E

M

V

V

G

H

M

V

V

L

F

E

Q

G

R

R

F

H

N

V

L

F

F

G

P

Q

H

L

M

S

T

V

V

E

L

D

N

N

N

L

I

R

T

S

L

G

A

G

P

F

M

-

K

V

V

R

R

R

K

L

W

S

P

R

R

Q

E

E

K

M

A

E

E

H

A

D

K

L

A

E

M

R

E

L

L

Y

L

A

D

W

K

F

V

P

-

R

G

L

L

K

K

T

D

K

K

R

A

H

H

T

A

R

Y

A

P

G

D

L

S

T

L

S

S

G

G

G

G

E

Q

Q

A

Q

Q

M

R

V

V

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

M

R

E

P

P

T

K

L

I

V

M

L

L

L

F

D

D

E

E

P

P

S

T

M

S

G

A

L

L

A

D

P

P

M

E

I

M

V

V

Q

G

E

E

I

V

F

L

E

S

I

V

I

M

G

K

Q

Q

L

L

N

A

R

N

E

E

Q

-

Q

G

V

M

S

T

F

M

L

V

I

V

A

V

E

T

Q

H

N

E

I

M

N

G

V

F

A

A

L

R

N

E

Y

V

A

G

S

D

H

R

G

V

Y

L

V

F

L

M

D

D

T

G

G

G

R

Y

V

I

A

I

L

E

S

E

G

G

S

K

A

P

A

E

E

D

L

L

L

F

A

D

R

R

binding adenosine-5'-diphosphate: V18 (≠ A29), S38 (≠ N49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
27% identity, 85% coverage: 25:241/255 of query aligns to 15:223/285 of 4yerA

query
sites
4yerA

G

G

A

D

I
x
F

L

E

A

A

V

V

A

K

G

G

V

V

S

S

L

F

S

S

V

V

P

K

K

K

G

G

A

E

T

I

V

F

A

A

L

F

L

L

G

G

A

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

I

K

H

A

M

I

L

S

T

G

T

L

L

V

L

R

K

A

P

E

-

R

-

A

-

E

-

V

-

S

T

R

S

G

G

T

K

I

A

E

W

Y

V

A

A

G

G

T

H

D

D

L

V

A

-

G

-

I

L

D

K

P

E

S

P

Q

R

R

E

V

V

R

R

Q

R

G

K

M

I

V

G

H

I

V

V

L

F

E

Q

G

D

R

Q

H

S

V

L

F

D

G

R

Q

E

L

L

S

T

V

A

E

Y

D

E

N

N

L

M

R

Y

S

I

G

H

G

G

F

K

V

I

R

Y

R

G

L

Y

S

G

R

G

Q

E

E

K

M

L

E

K

H

K

D

R

L

I

E

L

R

E

L

L

Y

L

A

E

W

F

F

V

P

E

R

L

L

L

K

E

T

F

K

K

R

D

H

K

T

P

R

V

A

K

G
x
T

L
x
F

T

-

S
|
S

G

G

G

G

E

M

Q

A

Q

R

M

R

V

L

A

E

I

I

G

A

R

R

A

S

L

L

M

I

T

H

R

E

P

P

T

E

L

V

V

L

L

F

L

L

D

D

E

E

P

P

S

T

M

I

G

G

L

L

A

D

P

P

M

H

I

T

V

R

Q

A

E

H

I

M

F

W

E

E

I

Y

I

I

G

S

Q

K

L

M

N

K

R

K

E

E

Q

H

Q

N

V

M

S

T

F

I

L

F

I

L

A

T

E

T

Q

H

N

Y

I

M

N

D

V

E

A

A

L

E

N

Q

Y

L

A

A

S

D

H

R

G

V

Y

A

V

I

L

I

D

D

T

H

G

G

R

K

V

I

A

I

L

A

S

L

G

G

S

T

A

P

A

T

E

E

L

L

binding adenosine-5'-diphosphate: F17 (≠ I27), N39 (= N49), G40 (= G50), G42 (= G52), K43 (= K53), T44 (≠ S54), T45 (= T55), T135 (≠ G152), F136 (≠ L153), S137 (= S155)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 14

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 89% coverage: 17:243/255 of query aligns to 6:230/343 of P30750

query
sites
P30750

N

N

D

I

I

T

E

K

V

V

I

F

Y

H

D

Q

G

G

A

T

-

R

-

T

I

I

L

Q

A

A

V

L

A

N

G

N

V

V

S

S

L

L

S

H

V

V

P

P

K

A

G

G

A

Q

T

I

V

Y

A

G

L

V

L

I

G

G

A

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

I

K

R

A

C

I

V

S

N

G

L

L

L

V

E

R

R

A

P

E

-

R

-

A

-

E

-

V

-

S

T

R

E

G

G

T

S

I

V

E

L

Y

V

A

D

G

G

T

Q

D

E

L

L

A

T

G

T

I

L

D

S

P

E

S

S

Q

E

-

L

-

T

-

K

-

A

R

R

V

R

R

Q

Q

I

G

G

M

M

V

I

-

F

-

Q

-

H

-

F

H

N

V

L

L

L

E

S

G

S

R

R

H

T

V

V

F

F

G

G

Q

N

L

V

S

A

V

L

E

P

-

L

-

E

-

L

D

D

N

N

L

T

R

P

S

K

G

D

G

E

F

V

V

K

R

R

R

R

L

V

S

T

R

-

Q

-

E

-

M

-

E

-

H

-

D

E

L

L

E

L

R

S

L

L

Y

V

A

G

W

-

F

-

P

-

R

-

L

L

K

G

T

D

K

K

R

H

H

D

T

S

R

Y

A

P

G

S

L

N

T

L

S

S

G

G

G

G

E

Q

Q

K

Q

Q

M

R

V

V

A

A

I

I

G

A

R

R

A

A

L

L

M

A

T

S

R

N

P

P

T

K

L

V

V

L

L

L

L

C

D

D

E
|
E

P

A

S

T

M

S

G

A

L

L

A

D

P

P

M

A

I

T

V

T

Q

R

E

S

I

I

F

L

E

E

I

L

I

L

G

K

Q

D

L

I

N

N

R

R

E

R

Q

L

Q

G

V

L

S

T

F

I

L

L

I

L

A

I

E

T

Q

H

N

E

I

M

N

D

V

V

A

V

L

K

N

R

Y

I

A

C

S

D

H

C

G

V

Y

A

V

V

L

I

D

S

T

N

G

G

R

E

V

L

A

I

L

E

S

Q

G

D

S

T

A

V

A

S

E

E

L

V

L

F

A

S

40:46 (vs. 49:55, 86% identical) binding
E166 (= E179) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
31% identity, 67% coverage: 30:200/255 of query aligns to 268:442/501 of P04983

query
sites
P04983

V

V

A

N

G

D

V

V

S

S

L

F

S

T

V

L

P

R

K

K

G

G

A

E

T

I

V

L

A

G

L

V

L

S

G

G

A

L

N

M

G

G

A

A

G

G

K

R

S

T

T

E

T

L

L

M

K

K

A

V

I

L

S

Y

G

G

L

A

V

L

R

P

A

R

E

-

R

-

A

-

E

-

V

-

S

T

R

S

G

G

T

Y

I

V

E

T

Y

L

A

D

G

G

T

H

D

E

L

V

A

V

G

T

I

R

D

S

P

P

S

Q

Q

D

R

G

V

L

R

A

Q

N

G

G

M

I

V

V

H

Y

V

I

L

S

E

E

G

D

R

R

H

K

-

R

-

D

-

G

-

L

V

V

F

L

G

G

Q

-

L

M

S

S

V

V

E

K

D

E

N

N

L

M

-

S

-

L

-

T

-

A

-

L

-

R

-

Y

-

F

-

S

R

R

S

A

G

G

G

G

F

S

V

L

R

K

R

H

L

A

S

D

R

E

Q

Q

E

Q

M

A

E

V

H

S

D

D

L

F

E

I

R

R

L

L

Y

F

A

-

W

-

F

-

P

-

R

N

L

V

K

K

T

T

K

P

R

S

H

M

T

E

R

Q

A

A

-

I

G

G

L

L

T

L

S

S

G

G

G

G

E

N

Q

Q

Q

Q

M

K

V

V

A

A

I

I

G

A

R

R

A

G

L

L

M

M

T

T

R

R

P

P

T

K

L

V

V

L

L

I

L

L

D

D

E

E

P

P

S

T

M

R

G

G

L

V

A

D

P

V

M

G

I

A

V

K

Q

K

E

E

I

I

F

Y

E

Q

I

L

I

I

G

N

Q

Q

L

F

N

K

Sites not aligning to the query:

43 K→R: Loss of transport.

Query Sequence

>GFF3277 FitnessBrowser__WCS417:GFF3277
MSQPAAESASQALLSVNDIEVIYDGAILAVAGVSLSVPKGATVALLGANGAGKSTTLKAI
SGLVRAERAEVSRGTIEYAGTDLAGIDPSQRVRQGMVHVLEGRHVFGQLSVEDNLRSGGF
VRRLSRQEMEHDLERLYAWFPRLKTKRHTRAGLTSGGEQQMVAIGRALMTRPTLVLLDEP
SMGLAPMIVQEIFEIIGQLNREQQVSFLIAEQNINVALNYASHGYVLDTGRVALSGSAAE
LLARGDLHDIYLGKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory