Comparing GFF3291 FitnessBrowser__psRCH2:GFF3291 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P23533 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Staphylococcus carnosus (strain TM300) (see paper)
40% identity, 58% coverage: 407:960/960 of query aligns to 4:570/573 of P23533
P08839 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 58% coverage: 408:959/960 of query aligns to 2:568/575 of P08839
2hwgA Structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system (see paper)
42% identity, 58% coverage: 408:960/960 of query aligns to 1:568/572 of 2hwgA
2wqdA Crystal structure of enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state (see paper)
40% identity, 58% coverage: 408:960/960 of query aligns to 4:570/570 of 2wqdA
2xz7A Crystal structure of the phosphoenolpyruvate-binding domain of enzyme i in complex with phosphoenolpyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
48% identity, 31% coverage: 658:958/960 of query aligns to 13:319/324 of 2xz7A
2xz9A Crystal structure from the phosphoenolpyruvate-binding domain of enzyme i in complex with pyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
48% identity, 31% coverage: 658:952/960 of query aligns to 6:306/317 of 2xz9A
5lu4A C4-type pyruvate phosphate dikinase: conformational intermediate of central domain in the swiveling mechanism (see paper)
30% identity, 39% coverage: 539:917/960 of query aligns to 409:836/850 of 5lu4A
Sites not aligning to the query:
P22983 Pyruvate, phosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Clostridium symbiosum (Bacteroides symbiosus) (see 4 papers)
30% identity, 39% coverage: 540:917/960 of query aligns to 409:855/874 of P22983
Sites not aligning to the query:
1kc7A Pyruvate phosphate dikinase with bound mg-phosphonopyruvate (see paper)
30% identity, 39% coverage: 540:917/960 of query aligns to 408:854/872 of 1kc7A
Sites not aligning to the query:
Q39735 Pyruvate, phosphate dikinase, chloroplastic; Cold-sensitive pyruvate, orthophosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) (see 2 papers)
27% identity, 44% coverage: 503:925/960 of query aligns to 437:945/953 of Q39735
Sites not aligning to the query:
5jvjB C4-type pyruvate phosphate dikinase: different conformational states of the nucleotide binding domain in the dimer (see paper)
28% identity, 39% coverage: 539:917/960 of query aligns to 336:781/797 of 5jvjB
Sites not aligning to the query:
5jvlA C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
28% identity, 39% coverage: 539:917/960 of query aligns to 409:858/874 of 5jvlA
Sites not aligning to the query:
O23404 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 39% coverage: 544:917/960 of query aligns to 505:946/963 of O23404
Q02KR1 Phosphoenolpyruvate synthase; PEP synthase; Pyruvate, water dikinase; EC 2.7.9.2 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
29% identity, 36% coverage: 557:899/960 of query aligns to 380:754/791 of Q02KR1
P11155 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Zea mays (Maize) (see 7 papers)
28% identity, 40% coverage: 544:925/960 of query aligns to 489:939/947 of P11155
Sites not aligning to the query:
1vbgA Pyruvate phosphate dikinase from maize (see paper)
28% identity, 40% coverage: 544:925/960 of query aligns to 416:866/874 of 1vbgA
Sites not aligning to the query:
5jvlB C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
27% identity, 38% coverage: 564:925/960 of query aligns to 108:512/520 of 5jvlB
Sites not aligning to the query:
1vbhA Pyruvate phosphate dikinase with bound mg-pep from maize (see paper)
27% identity, 40% coverage: 544:925/960 of query aligns to 407:854/862 of 1vbhA
Sites not aligning to the query:
1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
38% identity, 15% coverage: 1:141/960 of query aligns to 1:143/144 of 1j6tA
P37349 PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM; Dihydroxyacetone kinase subunit M; EC 2.7.1.121 from Escherichia coli (strain K12) (see paper)
28% identity, 35% coverage: 284:618/960 of query aligns to 157:462/472 of P37349
Sites not aligning to the query:
>GFF3291 FitnessBrowser__psRCH2:GFF3291
MLELNAQHIHMHQAAADKPAALALLGEVLVADGLVAAGYLEGLRAREAQGSTFLGQGIAI
PHGTPETRDQVFTTGVRLLHFPAGVDWGNGQLVYLAIGIAARSDEHLRLLQLLTRALGEG
DLSEGLQQAESPEAIIGLLQGAPQALALDGELVSLGVAADDFDELAWQGVKLLKRAQCVE
PGFNASLPLGQSLPLGDGLWWLSSEQSVQRPGLAFVTPVSNLEHQGQPLNGLFVLASMGE
AHQAMLERLCNLLIEGRGQELSQATSSRTVLEALGGDVPADWPSAQVPLANAHGLHARPA
KVLTEIAQAFEGEIRVRLAGTESAGVSVKSLSKLLAMGAHRGQLLEFMAEPAIASDALPA
LVRAVEEGLGEEVEPLPAPGEASEPPVTAQGPAESAIDQAALRAGDQVNGIAASPGIAIG
PVLVRKPQVIDYPKRGESPVIELQRLDAALDKVHADIGTLIDESQVASIRDIFTTHQAML
KDPALREEVQVRLQKGLSAEAAWMEEIESAAQQQEALHDKLLAERAADLRDVGRRVLACL
TGVEAEQAPDEPYILVMDEVAPSDVATLNAQRVAGILTAGGGATSHSAIIARALGIPAIV
GAGPGVLGLARNTLLLLDGERGELLVAPSGAQLEQARSERAAREERKHLANERRMDAAVT
RDGHPVEIAANIGAAGETPEAVAMGAEGIGLLRTELVFMNHSQAPNQATQEAEYRRVLEA
LEGRPLVVRTLDVGGDKPLPYWPMPAEENPFLGVRGIRLSLQRPDILETQLRALLASADG
RPLRIMFPMVGNIDEWRTAKAMVDRLRVELPVADLQVGIMIEIPSAALIAPVLAQEVDFF
SIGTNDLTQYTLAIDRGHPTLSGQADGLHPAVLRLIGMTVEAAHAHGKWVGVCGELAADA
LAVPLLVGLGVDELSVSARSIALVKARVRELDFAACQRLAQQALMLPGAHEVRAFVGEHC
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory