SitesBLAST

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 89% coverage: 18:403/435 of query aligns to 69:472/582 of Q9JI12

query
sites
Q9JI12

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R88 (= R37) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
H128 (≠ F62) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R116) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E123) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ L255) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
24% identity, 89% coverage: 18:403/435 of query aligns to 11:414/452 of 7t3nA

query
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7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y69), Y137 (= Y127), Y165 (≠ F155), R264 (≠ L255), F268 (≠ L259), Y269 (≠ W260)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: R12 (= R19), Y13 (≠ F20), E152 (= E142), G163 (= G153)

8u3gA Structure of naag-bound sialin
24% identity, 96% coverage: 19:435/435 of query aligns to 4:427/427 of 8u3gA

query
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8u3gA

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binding aspartic acid: F15 (≠ V30), Y19 (= Y34), F118 (≠ Y127)
binding glutamic acid: S350 (= S362), H353 (≠ S365), L365 (≠ G377)

8u3hA Structure of fmoc-leu-oh bound sialin
24% identity, 96% coverage: 19:435/435 of query aligns to 4:425/425 of 8u3hA

query
sites
8u3hA

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S

I

V

A

A

A

L

P

V

A

D

L

M

-

V

-

D

T

S

S

K

E

K

L

Y

G

Q

I

W

D

D

P

A

V

E

H

T

V

Q

G

G

L

W

I

I

F

L

S

G

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

M

T

Q

Q

I

I

P

P

G

G

W

Y

L

V

V

A

D

S

R

K

V

I

P

G

R

K

I

M

L

L

Y

L

T

G

A

F

A

G

L

I

L

L

L

G

W

T

S

A

I

V

A

L

T

T

V

L

M

F

L

T

G

P

F

I

A

A

S

D

F

L

I

G

A

V

-

G

-

P

L

L

F

I

V

V

L

L

R

R

M

A

A

L

V

E

G

G

A

L

L

G

E

E

A

G

P

V

A

T

Y

F

P

P

I

A

N

M

S

H

R

A

V

M

V

W

T

S

W

W

F

A

P

P

E

P

R

L

E

E

R

R

A

S

T

K

A

L

I

L

G

S

V

I

Y

S

T

Y

S

A

G

G

Q

A

F

Q

V

L

G

G

L

T

A

V

F

I

L

S

T

L

P

P

V

L

L

S

A

G

W

I

L

I

Q

C

H

Y

A

Y

F

M

G

N

W

W

H

T

M

Y

V

V

F

F

V

Y

A

F

T

F

G

G

G

T

V

I

G

G

I

I

V

F

W

W

A

F

V

L

I

L

W

W

-

I

Y

W

A

L

V

V

Y

S

R

D

E

T

P

P

K

Q

E

K

F

H

K

K

G

R

A

I

N

S

T

H

A

Y

E

E

I

K

D

E

L

Y

I

I

R

-

E

-

G

-

L

L

V

S

D

S

M

L

Q

R

E

N

K

Q

-

L

A

S

K

Q

A

K

P

S

F

V

S

P

W

W

V

V

D

P

L

-

G

-

I

-

V

I

L

L

S

K

K

S

R

L

K

P

L

L

W

W

G

A

I

I

Y

V

L

V

G

A

Q
x
H

F

F

C

S

L
x
Y

N

N

S

W

T

T

L

F

W
x
Y

F

T

F

L

L

L

T

T

W

L

F

L

P

P

T

T

Y

Y

L

M

V

K

K

E

Y

I

R

L

G

R

M

F

D

N

F

V

I

Q

K

E

S

N

G

G

L

F

L

L

A

S

S

S

L

L

P

P

F

Y

L

L

A

G

A

S

F

W

V

L

G

C

V
x
M

L

I

C

L

S

S

G

G

I

Q

F

A

S

A

D

D

W

N

L

L

I

R

R

A

R

K

G

W

-

N

-

F

A

S

S

T

V

L

G

C

F

V

A

R

R

R

K

I

L

F

P

S

I

L

I

I

G

G

G

M

L

I

-

G

-

P

-

A

-

V

-

F

L

L

I

V

S

A

T

A

A

G

I

F

I

I

G

G

A

C

N

D

Y

Y

V

-

D

-

S

-

T

-

A

S

W

L

V

A

I

V

A

A

F

F

L

L

A

T

V

I

A

S

F

T

G

L

N

G

G

G

L

F

A

C

S
|
S

I

S

T

G

W

F

S

S

L

I

V

N

S

H

T

L

L

D

A

I

P

A

A

P

R

S

L

Y

L

A

G

G

L

I

T

L

G

L

G

G

V

I

F

T

N

N

F

T

I

F

G

A

N

T

L

I

S

P

A

G

I

M

A

V

T

G

P

P

I

V

V

I

I

A

G

K

F

S

L

L

A

T

S

P

G

D

D

N

S

T

F

V

A

G

P

E

-

W

-

Q

A

T

I

V

T

F

Y

Y

I

I

-

A

A

A

V

A

L

I

A

N

L

V

L

F

G

G

A

A

L

I

S

F

Y

F

V

T

L

L

L

F

V

A

-

K

G

G

K

E

V

V

E

Q

R

N

I

W

E

A

L

L

K

N

binding leucine: M279 (≠ V296), S344 (= S358)
binding Fluorenylmethyloxycarbonyl chloride: Y19 (= Y34), H235 (≠ Q252), Y238 (≠ L255), Y243 (≠ W260)

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 87% coverage: 54:431/435 of query aligns to 104:484/495 of Q5Q0U0

query
sites
Q5Q0U0

D

D

P

A

V

E

H

T

V

Q

G

G

L

W

I

I

F

L

S

G

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

A

V

M

T

Q

Q

I

I

P

P

G

G

W

Y

L

M

V

A

D

S

R

R

V

I

P

G

R
x
K

I

L

L

L

Y

L

T

G

A

L

A

G

L

I

L

L

L

G

W

T

S

A

I

V

A

F

T

T

V

L

M

F

L

T

G

P

F

L

A

A

S

D

F

L

-

G

-

V

I

V

A

A

L

L

F

I

V

V

L

L

R
|
R

M

A

A

L

V
x
E

G
|
G

A

L

L

G

E
|
E

A
x
G

P

V

A

T

Y
x
F

P
|
P

I

A

N

M

S
x
H

R

A

V

M

V
x
W

T

S

W

W

F

A

P

P

E

P

R

L

E

E

R

R

A

S

T

K

A

L

I

L

G

T

V

I

Y

S

T

Y

S

A

G

G

Q

A

F

Q

V

L

G

G

L

T

A

V

F

I

L

S

T

L

P

P

V

L

L

S

A

G

W

I

L

I

Q

C

H

Y

A

Y

F

M

G

N

W

W

H

T

M

Y

V

V

F

F

V

Y

A

L

T

F

G

G

G

L

V

V

G

G

I

I

V

V

W

W

A

F

V

I

I

L

W

W

-

M

Y

W

A

I

V

V

Y

S

R

D

E

T

P

P

K

E

E

T

F

H

K

K

G

T

A

I

N

S

T

H

A

Y

E

E

I

K

D

E

L

Y

I

I

R

V

E
x
S

G

-

G
x
S

L
|
L

V
x
K

D
x
N

M

Q

Q

L

E

S

K

S

A

Q

A

K

K

V

A

V

P

P

F

-

S

-

W

W

V

V

D

S

L

-

G

-

I

-

V

I

L

L

S

K

K

S

R

L

K

P

L

L

W

W

G

A

I

I

Y

V

L

V

G

A

Q

H

F

F

C

S

L

Y

N

N

S

W

T

T

L

F

W

Y

F

T

F

L

L

L

T

T

W

L

F

L

P

P

T

T

Y

Y

L

M

V

K

K

E

Y

I

R

L

G

R

M

F

D

N

F

V

I

Q

K

E

S

N

G

G

L

F

L

L

A

S

S

A

L

L

P
|
P

F

Y

L

F

A

G

A

C

F

W

V

L

G

C

V

M

L

I

C

V

S

C

G

G

I

Q

F

A

S

A

D

D

W

Y

L

L

I

R

R

V

R

K

-

W

-

N

G

F

A

S

S

T

V

I

G

S

F

V

A

R

R

R

K

I

L

F

P

S

I

L

I

I

G
|
G

G

M

L

V

-

G

-

P

-

A

-

V

-

F

L

L

I

V

S

A

T

A

A

G

I

F

I

I

G

G

A

C

N

D

Y

Y

V

-

D

-

S

-

T

-

A

S

W

L

V

A

I

V

A

A

F

F

L

L

A

T

V

I

A

S

F

T

G

L

N

G

G

G

L

F

A

A

S

S

I

S

T

G

W

F

S

S

L

I

V

N

S

H

T

L

L

D

A

I

P

A

A

P

R

S

L

Y

L

A

G

G

L

I

T

L

G

L

G

G

V

I

F

T

N

N

F

T

I

F

G

A

N

T

L

I

S

P

A

G

I

M

A

I

T

G

P

P

I

I

V

I

I

A

G

K

F

S

L

L

A

T

S

P

G

D

D

N

S

T

F

I

A

K

P

E

A

W

I

Q

T

T

Y

V

I

F

A

C

V

I

L

A

A

A

-

A

-

I

-

N

L

V

L

F

G

G

A

A

L

I

S

F

Y

F

V

T

L

L

L

F

V

A

-

K

G

G

K

E

V

V

E

Q

R

N

K136 (≠ R86) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R116) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ V119) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G120) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E123) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A124) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ Y127) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P128) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ S131) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ V134) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
S--SLKN 268:272 (≠ EGGGLVD 215:221) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P288) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G323) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
24% identity, 87% coverage: 54:430/435 of query aligns to 104:483/495 of Q8BN82

query
sites
Q8BN82

D

D

P

A

V

E

H

T

V

Q

G

G

L

W

I

I

F

L

S

G

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

A

V

M

T

Q

Q

I

I

P

P

G

G

W

Y

L

I

V

A

D

S

R

R

V

V

P

G

R

K

I

L

L

L

Y

L

T

G

A

L

A

G

L

I

L

L

L

G

W

T

S

S

I

V

A

F

T

T

V

L

M

F

L

T

G

P

F

L

A

A

S

D

F

L

-

G

-

V

I

V

A

T

L

L

F

V

V

V

L

L

R

R

M

A

A

L

V

E

G

G

A

L

L

G

E

E

A

G

P

V

A

T

Y

F

P

P

I

A

N

M

S
x
H

R

A

V

M

V

W

T

S

W

W

F

A

P

P

E

P

R

L

E

E

R

R

A

S

T

K

A

L

I

L

G

T

V

I

Y

S

T

Y

S

A

G

G

Q

A

F

Q

V

L

G

G

L

T

A

V

F

I

L

S

T

L

P

P

V

L

L

S

A

G

W

I

L

I

Q

C

H

Y

A

Y

F

M

G

N

W

W

H

T

M

Y

V

V

F

F

V

Y

A

L

T

F

G

G

G

I

V

V

G

G

I

I

V

V

W

W

A

F

V

I

I

L

W

W

-

M

Y

W

A

I

V

V

Y

S

R

D

E

T

P

P

K

E

E

T

F

H

K

K

G

T

A

I

N

S

T

H

A

Y

E

E

I

K

D

E

L

Y

I

I

R

V

E

S

G

S

G

-

L

-

V

L

D

K

M

N

Q

Q

E

L

K

S

A

S

A

Q

K

K

A

V

P

-

F

-

S

-

W

-

V

V

D

P

L

W

G

G

I

S

V

I

L

L

S

K

K

S

R

L

K

P

L

L

W

W

G

A

I

I

Y

V

L

V

G

A

Q

H

F

F

C

S

L

Y

N

N

S

W

T

S

L

F

W

Y

F

T

F

L

L

L

T

T

W

L

F

L

P

P

T

T

Y

Y

L

M

V

K

K

E

Y

I

R

L

G

R

M

F

D

N

F

V

I

Q

K

E

S

N

G

G

L

F

L

L

A

S

S

A

L

L

P

P

F

Y

L

F

A

G

A

C

F

W

V

L

G

C

V

M

L

I

C

L

S

C

G

G

I

Q

F

A

S

A

D

D

W

Y

L

L

I

R

R

V

R

K

-

W

-

N

G

F

A

S

S

T

V

I

G

S

F

V

A

R

R

R

K

I

L

F

P

S

I

L

I

V

G

G

G

M

L

V

-

G

-

P

-

A

-

V

-

F

L

L

I

V

S

A

T

A

A

G

I

F

I

I

G

G

A

C

N

D

Y

Y

V

-

D

-

S

-

T

-

A

S

W

L

V

A

I

V

A

A

F

F

L

L

A

T

V

I

A

S

F

T

G

L

N

G

G

G

L

F

A

A

S

S

I

S

T

G

W

F

S

S

L

I

V

N

S

H

T

L

L

D

A

I

P

A

A

P

R

S

L

Y

L

A

G

G

L

I

T

L

G

L

G

G

V

I

F

T

N

N

F

T

I

F

G

A

N

T

L

I

S

P

A

G

I

M

A

T

T

G

P

P

I

I

V

I

I

A

G

K

F

S

L

L

A

T

S

P

G

D

D

N

S

T

F

I

A

R

P

E

A

W

I

Q

T

T

Y

V

I

F

A

C

V

I

L

A

A

A

-

A

-

I

-

N

L

V

L

F

G

G

A

A

L

I

S

F

Y

F

V

T

L

L

L

F

V

A

-

K

G

G

K

E

V

V

E

Q

H183 (≠ S131) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
24% identity, 87% coverage: 54:431/435 of query aligns to 104:484/495 of Q9NRA2

query
sites
Q9NRA2

D

D

P

A

V

E

H

T

V

Q

G

G

L

W

I

I

F

L

S

G

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

M

T

Q

Q

I

I

P

P

G

G

W

Y

L

V

V

A

D

S

R

K

V

I

P

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N

K136 (≠ R86) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ S131) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 146:147) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
I-----L 266:267 (≠ IREGGGL 213:219) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ VDMQE 220:224) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G282) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P288) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G323) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 71% coverage: 4:310/435 of query aligns to 2:338/493 of Q03567

query
sites
Q03567

E

E

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G

N69 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
22% identity, 80% coverage: 54:403/435 of query aligns to 106:455/497 of Q9Y2C5

query
sites
Q9Y2C5

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