SitesBLAST
Comparing GFF3348 FitnessBrowser__Marino:GFF3348 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
58% identity, 100% coverage: 2:342/342 of query aligns to 3:329/331 of 4yi7A
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
38% identity, 98% coverage: 7:341/342 of query aligns to 4:325/325 of 5nofA
1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
36% identity, 93% coverage: 1:319/342 of query aligns to 1:312/344 of 1zxyA
- binding magnesium ion: D223 (= D226), E224 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V80), G79 (= G81), D83 (= D85), T87 (≠ L89), N89 (= N91), S91 (= S93), T92 (≠ S94), K106 (= K109), S118 (= S121), D223 (= D226), E224 (= E227)
2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
36% identity, 93% coverage: 1:319/342 of query aligns to 1:312/345 of 2gvqD
P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
36% identity, 93% coverage: 1:319/342 of query aligns to 1:312/345 of P50384
- T87 (≠ L89) binding 5-phospho-alpha-D-ribose 1-diphosphate
- K106 (= K109) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
- H107 (= H110) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
- S118 (= S121) binding 5-phospho-alpha-D-ribose 1-diphosphate
- H154 (= H157) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
- R164 (= R167) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
- D223 (= D226) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
- E224 (= E227) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
34% identity, 96% coverage: 7:336/342 of query aligns to 6:328/330 of 1kgzB
- binding manganese (ii) ion: S92 (= S93), D222 (= D226), E223 (= E227), E223 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (= V80), G82 (= G83), G83 (= G84), N90 (= N91), I91 (≠ V92), S92 (= S93), T93 (≠ S94), K108 (= K109), S118 (= S121)
1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
35% identity, 93% coverage: 1:319/342 of query aligns to 1:308/339 of 1gxbA
3gbrA Anthranilate phosphoribosyl-transferase (trpd) double mutant d83g f149s from s. Solfataricus (see paper)
35% identity, 93% coverage: 1:319/342 of query aligns to 1:309/341 of 3gbrA
- binding manganese (ii) ion: S91 (= S93), D220 (= D226), E221 (= E227), E221 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V80), G79 (= G81), T80 (= T82), G81 (= G83), N89 (= N91), V90 (= V92), S91 (= S93), T92 (≠ S94), K106 (= K109), G114 (= G120)
4zojA Methylsulfanyl-containing inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
37% identity, 99% coverage: 3:340/342 of query aligns to 3:340/341 of 4zojA
- active site: V82 (= V80)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfanyl)phenyl]amino}benzoic acid: N114 (= N112), A155 (= A153), P156 (= P154), H159 (= H157), Y162 (≠ M160), R169 (= R167), G182 (= G180)
- binding magnesium ion: S95 (= S93), D227 (= D226), E228 (= E227), E228 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G81), G85 (= G83), G86 (= G84), N93 (= N91), S95 (= S93), T96 (≠ S94), K111 (= K109), R115 (= R113), A116 (= A114), A117 (≠ V115), S119 (= S117)
4zokA Methylsulfonyl-containing inhibitor bound in the substrate capture site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
36% identity, 99% coverage: 3:340/342 of query aligns to 3:341/342 of 4zokA
- active site: V82 (= V80)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfonyl)phenyl]amino}benzoic acid: P156 (= P154), R163 (≠ K161), A166 (≠ I164), A167 (≠ G165)
- binding magnesium ion: S95 (= S93), E228 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G84), N93 (= N91), S95 (= S93), T96 (≠ S94), K111 (= K109), A116 (= A114), A117 (≠ V115), S119 (= S117), G122 (= G120)
4zofA Lobenzarit-like inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
36% identity, 99% coverage: 3:340/342 of query aligns to 3:341/342 of 4zofA
- active site: V82 (= V80)
- binding 2-[(2-carboxy-5-nitrophenyl)amino]-3-methylbenzoic acid: G113 (= G111), N114 (= N112), A155 (= A153), H159 (= H157), Y162 (≠ M160), R169 (= R167), G182 (= G180)
- binding magnesium ion: S95 (= S93), D227 (= D226), E228 (= E227), E228 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G81), G85 (= G83), G86 (= G84), N93 (= N91), S95 (= S93), T96 (≠ S94), K111 (= K109), A116 (= A114), A117 (≠ V115), S119 (= S117), G122 (= G120)
5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
36% identity, 99% coverage: 3:340/342 of query aligns to 4:343/344 of 5c7sA
- active site: V83 (= V80)
- binding manganese (ii) ion: G84 (= G81), S96 (= S93), D228 (= D226), E229 (= E227), E229 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (= V80), G84 (= G81), G86 (= G83), G87 (= G84), N94 (= N91), S96 (= S93), T97 (≠ S94), K112 (= K109), N115 (= N112), S120 (= S117)
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
36% identity, 99% coverage: 3:340/342 of query aligns to 3:342/344 of 3r88B
- active site: V82 (= V80)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N112), A155 (= A153), P156 (= P154), H159 (= H157), Y162 (≠ M160), R163 (≠ K161), A166 (≠ I164)
- binding magnesium ion: S95 (= S93), D227 (= D226), E228 (= E227), E228 (= E227)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (= V80), G83 (= G81), G85 (= G83), G86 (= G84), N93 (= N91), S95 (= S93), T96 (≠ S94), K111 (= K109), N114 (= N112), A117 (≠ V115), S118 (= S116), S119 (= S117)
5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
36% identity, 99% coverage: 3:340/342 of query aligns to 3:342/343 of 5c1rA