SitesBLAST
Comparing GFF3358 FitnessBrowser__Phaeo:GFF3358 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
38% identity, 98% coverage: 6:286/288 of query aligns to 4:292/306 of 4whxA
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
40% identity, 90% coverage: 7:264/288 of query aligns to 3:265/305 of 2ej0B
- active site: F35 (= F39), G37 (= G41), K158 (= K157), E192 (= E191), L215 (= L214)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R58), Y163 (= Y162), E192 (= E191), G195 (= G194), E196 (≠ A195), L215 (= L214), G217 (= G216), I218 (= I217), T219 (= T218), G254 (= G253), T255 (= T254)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
37% identity, 97% coverage: 11:288/288 of query aligns to 4:283/290 of 5mr0D
- active site: F32 (= F39), G34 (= G41), K150 (= K157), E183 (= E191), L206 (= L214)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R58), G100 (= G108), L101 (≠ V109), K150 (= K157), Y154 (= Y162), E183 (= E191), G186 (= G194), D187 (≠ A195), L206 (= L214), I209 (= I217), T210 (= T218), G245 (= G253), T246 (= T254)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1iyeA
- active site: F33 (= F39), G35 (= G41), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L214)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R58), Y92 (= Y94), Y126 (= Y128), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1iydA
- active site: F33 (= F39), G35 (= G41), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L214)
- binding glutaric acid: Y92 (= Y94), Y126 (= Y128), A255 (= A255)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1i1mA
- active site: K156 (= K157)
- binding 4-methyl valeric acid: Y92 (= Y94), K156 (= K157), T254 (= T254), A255 (= A255)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1i1lA
- active site: K156 (= K157)
- binding 2-methylleucine: Y92 (= Y94), K156 (= K157), T254 (= T254), A255 (= A255)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
39% identity, 89% coverage: 10:264/288 of query aligns to 4:257/290 of 5e25A
- active site: F33 (= F39), G35 (= G41), K151 (= K157), E184 (= E191), L207 (= L214)
- binding 2-oxoglutaric acid: Y88 (= Y94), K151 (= K157), T247 (= T254), A248 (= A255)
- binding pyridoxal-5'-phosphate: R52 (= R58), K151 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), D188 (≠ A195), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
37% identity, 96% coverage: 11:286/288 of query aligns to 9:286/301 of 6thqB
- active site: F37 (= F39), K156 (= K157), E190 (= E191), L214 (= L214)
- binding pyridoxal-5'-phosphate: R60 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R58), Y97 (= Y94), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 2ej3A
- active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G194), G246 (= G253), T247 (= T254), A248 (= A255)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 2eiyA
- active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
- binding 4-methyl valeric acid: F35 (= F39), Y94 (= Y94), T247 (= T254), A248 (= A255)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), E188 (≠ A195), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 1wrvA
- active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), T247 (= T254)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
38% identity, 90% coverage: 7:264/288 of query aligns to 3:254/294 of 2ej2A
- active site: F35 (= F39), G37 (= G41), K147 (= K157), E181 (= E191), L204 (= L214)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R58), Y94 (= Y94), Y152 (= Y162), E181 (= E191), G184 (= G194), E185 (≠ A195), L204 (= L214), G206 (= G216), I207 (= I217), T208 (= T218), T244 (= T254), A245 (= A255)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
27% identity, 96% coverage: 11:286/288 of query aligns to 6:290/307 of 6q8eA
- active site: F34 (= F39), K156 (= K157), E190 (= E191), L214 (= L214)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), S194 (≠ A195), C195 (≠ N196), L214 (= L214), S216 (≠ G216), I217 (= I217), T218 (= T218), G254 (= G253), T255 (= T254)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
26% identity, 96% coverage: 11:286/288 of query aligns to 6:290/309 of 7neaA
- active site: F34 (= F39), K156 (= K157), E190 (= E191), L214 (= L214)
- binding pyridoxal-5'-phosphate: R59 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), S194 (≠ A195), L214 (= L214), S216 (≠ G216), I217 (= I217), T218 (= T218), T255 (= T254)
7dbeB Structure of a novel transaminase
27% identity, 88% coverage: 9:261/288 of query aligns to 39:292/332 of 7dbeB
- binding pyridoxal-5'-phosphate: R88 (= R58), K190 (≠ A158), E223 (= E191), G226 (= G194), F227 (≠ A195), N228 (= N196), L245 (= L214), G247 (= G216), I248 (= I217), T249 (= T218), T285 (= T254)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
27% identity, 94% coverage: 12:282/288 of query aligns to 8:288/308 of 6h65C
- active site: F35 (= F39), K158 (= K157), E192 (= E191), L216 (= L214)
- binding pyridoxal-5'-phosphate: R60 (= R58), K158 (= K157), Y163 (= Y162), E192 (= E191), A196 (= A195), L216 (= L214), S218 (≠ G216), V219 (≠ I217), T220 (= T218), G256 (= G253), T257 (= T254)
7p3tB Transaminase of gamma-proteobacterium (see paper)
27% identity, 95% coverage: 10:282/288 of query aligns to 5:279/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R58), K153 (= K157), R157 (≠ L161), E186 (= E191), S187 (≠ A192), A188 (≠ T193), A189 (≠ G194), S190 (≠ A195), G210 (= G216), I211 (= I217), T212 (= T218), T248 (= T254)
3cswC Crystal structure of a putative branched-chain amino acid aminotransferase (tm0831) from thermotoga maritima at 2.15 a resolution
27% identity, 96% coverage: 10:286/288 of query aligns to 4:265/275 of 3cswC
- active site: Y33 (≠ F39), K135 (= K157), E166 (= E191), L190 (= L214)
- binding pyridoxal-5'-phosphate: R52 (= R58), K135 (= K157), E166 (= E191), F169 (≠ G194), S170 (≠ A195), L190 (= L214), G192 (= G216), I193 (= I217), T194 (= T218), T230 (= T254)
6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
24% identity, 95% coverage: 9:282/288 of query aligns to 28:305/321 of 6fteB
- active site: Y58 (≠ F39), K179 (= K157), E212 (= E191), L234 (= L214)
- binding pyridoxal-5'-phosphate: R77 (= R58), K179 (= K157), E212 (= E191), F216 (≠ A195), N217 (= N196), L234 (= L214), G236 (= G216), V237 (≠ I217), T238 (= T218), T274 (= T254)
Query Sequence
>GFF3358 FitnessBrowser__Phaeo:GFF3358
MAGYDDRDGLIWMDGEMVNWRDAKVHILSHAMHYASSVFEGERAYNGKIFKSREHSERLI
ASAEALDMPMPYTVDQIEAAKEETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAI
AVWGWGAYYGDAKMQGAKLDIAEWKRPSPETIPVHAKAAGLYMICTISKHKAEAKGCSDA
LFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIGMLKDRGITVHERHIMP
EELDGFEQCWLTGTAAEVTPVGQIGPHSFEVGELTQDIAKAYEELVRS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory