SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3358 FitnessBrowser__Phaeo:GFF3358 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
38% identity, 98% coverage: 6:286/288 of query aligns to 4:292/306 of 4whxA

query
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4whxA

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active site: F37 (= F39), K160 (= K157), E194 (= E191), L217 (= L214)
binding alanine: Y97 (= Y94), K160 (= K157), G197 (= G194), T257 (= T254), A258 (= A255)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
40% identity, 90% coverage: 7:264/288 of query aligns to 3:265/305 of 2ej0B

query
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2ej0B

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active site: F35 (= F39), G37 (= G41), K158 (= K157), E192 (= E191), L215 (= L214)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R58), K158 (= K157), Y163 (= Y162), E192 (= E191), G195 (= G194), E196 (≠ A195), N197 (= N196), L215 (= L214), G217 (= G216), I218 (= I217), T219 (= T218), G254 (= G253), T255 (= T254)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
37% identity, 97% coverage: 11:288/288 of query aligns to 4:283/290 of 5mr0D

query
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5mr0D

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active site: F32 (= F39), G34 (= G41), K150 (= K157), E183 (= E191), L206 (= L214)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: F32 (= F39), R51 (= R58), R89 (= R96), G100 (= G108), L101 (≠ V109), K150 (= K157), Y154 (= Y162), E183 (= E191), G186 (= G194), D187 (≠ A195), N188 (= N196), L206 (= L214), G208 (= G216), I209 (= I217), T210 (= T218), G245 (= G253), T246 (= T254)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1iyeA

query
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1iyeA

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active site: F33 (= F39), G35 (= G41), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L214)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R58), Y92 (= Y94), R94 (= R96), Y126 (= Y128), K156 (= K157), Y161 (= Y162), E190 (= E191), A192 (≠ T193), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1iydA

query
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1iydA

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S

M

E

Q

R
|
R

L

L

I

H

A

D

S

S

A

A

E

K

A

I

L

Y

D

R

M

F

P

P

M

V

P

S

Y

Q

T

S

V

I

D

D

Q

E

I

L

E

M

A

E

A

A

K

C

E

R

E

D

T

V

L

I

K

R

A

K

S

N

G

N

L

L

Q

T

D

S

A

A

Y
|
Y

V

I

R
|
R

A

P

V

L

V

I

W

F

R

V

G

G

S

D

G

-

E

V

D

G

M

M

G

G

V

V

-

N

A

P

S

P

A

A

K

G

N

Y

P

S

V

T

R

D

M

V

A

I

I

I

A

A

V

A

W

F

G

P

W
|
W

G

G

A

A

Y
|
Y

Y

L

G

G

-

A

D

E

A

A

K

L

M

E

Q

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

E

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A
|
A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

I

L

S

V

K

G

H

S

K

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

K

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

K

K

D

E

R

L

G

G

I

I

T

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

D

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

-

P

-

H

D

S

G

F

I

E

Q

V

V

G

G

E

E

-

G

-

R

-

C

-

G

-

P

L

V

T

T

Q

K

D

R

I

I

A

Q

K

Q

A

A

Y

F

active site: F33 (= F39), G35 (= G41), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L214)
binding glutaric acid: G35 (= G41), Y92 (= Y94), R94 (= R96), W123 (= W125), Y126 (= Y128), K156 (= K157), Y161 (= Y162), T254 (= T254), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1i1mA

query
sites
1i1mA

I

I

W

W

M

F

D

N

G

G

E

E

M

M

V

V

N

R

W

W

R

E

D

D

A

A

K

K

V

V

H

H

I

V

L

M

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F

F

E

E

G
|
G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

K

R

S

H

R

R

E

E

H

H

S

M

E

Q

R
|
R

L

L

I

H

A

D

S

S

A

A

E

K

A

I

L

Y

D

R

M

F

P

P

M

V

P

S

Y

Q

T

S

V

I

D

D

Q

E

I

L

E

M

A

E

A

A

K

C

E

R

E

D

T

V

L

I

K

R

A

K

S

N

G

N

L

L

Q

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

V

I

W

F

R

V

G

G

S

D

G

-

E

V

D

G

M

M

G

G

V

V

-

N

A

P

S

P

A

A

K

G

N

Y

P

S

V

T

R

D

M

V

A

I

I

I

A

A

V

A

W

F

G

P

W

W

G

G

A

A

Y

Y

Y

L

G

G

-

A

D

E

A

A

K

L

M

E

Q

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

E

S

W

W

K

N

R
|
R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

I

L

S

V

K

G

H

S

K

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

K

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

K

K

D

E

R

L

G

G

I

I

T

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

D

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

-

P

-

H

D

S

G

F

I

E

Q

V

V

G

G

E

E

-

G

-

R

-

C

-

G

-

P

L

V

T

T

Q

K

D

R

I

I

A

Q

K

Q

A

A

Y

F

active site: K156 (= K157)
binding 4-methyl valeric acid: G35 (= G41), Y92 (= Y94), K156 (= K157), Y161 (= Y162), G193 (= G194), T254 (= T254), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R58), R145 (= R146), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
38% identity, 94% coverage: 11:282/288 of query aligns to 5:285/304 of 1i1lA

query
sites
1i1lA

I

I

W

W

M

F

D

N

G

G

E

E

M

M

V

V

N

R

W

W

R

E

D

D

A

A

K

K

V

V

H

H

I

V

L

M

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F

F

E

E

G
|
G

E

I

R

R

A

C

Y

Y

N

D

G

S

K

H

-

K

-

G

-

P

-

V

I

V

F

F

K

R

S

H

R

R

E

E

H

H

S

M

E

Q

R
|
R

L

L

I

H

A

D

S

S

A

A

E

K

A

I

L

Y

D

R

M

F

P

P

M

V

P

S

Y

Q

T

S

V

I

D

D

Q

E

I

L

E

M

A

E

A

A

K

C

E

R

E

D

T

V

L

I

K

R

A

K

S

N

G

N

L

L

Q

T

D

S

A

A

Y
|
Y

V

I

R

R

A

P

V

L

V

I

W

F

R

V

G

G

S

D

G

-

E

V

D

G

M

M

G

G

V

V

-

N

A

P

S

P

A

A

K

G

N

Y

P

S

V

T

R

D

M

V

A

I

I

I

A

A

V

A

W

F

G

P

W
|
W

G

G

A

A

Y
|
Y

Y

L

G

G

-

A

D

E

A

A

K

L

M

E

Q

Q

G

G

A

I

K

D

L

A

D

M

I

V

A

S

E

S

W

W

K

N

R

R

P

A

S

A

P

P

E

N

T

T

I

I

P

P

V

T

H

A

A

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

I

L

S

V

K

G

H

S

K

E

A

A

E

R

A

R

K

H

G

G

C

Y

S

Q

D

E

A

G

L

I

F

A

M

L

D

D

Y

V

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

K

K

D

D

G

G

E

V

V

L

H

F

T

T

P

P

K

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

G

K

M

L

L

A

K

K

D

E

R

L

G

G

I

I

T

E

V

V

H

R

E

E

R

Q

H

V

I

L

M

S

P

R

E

E

E

S

L

L

D

Y

G

L

F

A

E

D

Q

E

C

V

W

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

Q

S

I

V

G

-

P

-

H

D

S

G

F

I

E

Q

V

V

G

G

E

E

-

G

-

R

-

C

-

G

-

P

L

V

T

T

Q

K

D

R

I

I

A

Q

K

Q

A

A

Y

F

active site: K156 (= K157)
binding 2-methylleucine: G35 (= G41), Y92 (= Y94), W123 (= W125), Y126 (= Y128), K156 (= K157), G193 (= G194), T254 (= T254), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
39% identity, 89% coverage: 10:264/288 of query aligns to 4:257/290 of 5e25A

query
sites
5e25A

L

L

I

V

W

Y

M

M

D

N

G

G

E

E

M

F

V

V

N

P

W

E

R

S

D

Q

A

A

K

K

V

V

H

S

I

V

L

F

S

D

H

H

A

G

M

F

H

L

Y

Y

A

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

N

G

G

K

K

I

V

F

F

K

K

S

L

R

Y

E

E

H

H

S

I

E

D

R
|
R

L

L

I

Y

A

D

S

C

A

A

E

R

A

V

L

I

D

D

M

L

P

K

M

I

P

P

Y

L

T

S

V

K

D

E

Q

E

I

F

E

A

A

E

A

A

K

I

E

L

E

E

T

T

L

L

K

R

A

R

S

N

G

N

L

L

Q

R

D

D

A

A

Y
|
Y

V

I

R
|
R

A

P

V

I

V

V

W

T

R

R

G

G

S

A

G

G

E

-

D

D

M

L

G

G

V

L

A

D

S

P

A

R

K

K

N

C

P

P

V

S

R

P

M

N

A

V

I

I

A

I

V

I

W

T

G

K

-

P

W

W

G

G

A

K

Y

L

Y

Y

G

G

D

D

A

L

K

Y

M

E

Q

K

G

G

A

L

K

K

L

A

D

I

I

T

A

V

E

A

W

I

K

R

R

R

P

N

S

A

P

I

E

D

T

S

I

L

P

P

V

P

H

N

A

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

I

L

S

A

K

K

H

I

K

E

A

A

E

N

A

A

K

K

G

G

C

G

S

D

D

E

A

A

L

I

F

F

M

L

D

D

Y

H

R

N

G

G

Y

Y

V

I

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N
|
N

I

I

F

F

F

I

V

V

K

K

D

N

G

G

E

T

V

I

H

T

T

T

P

P

K

P

P

T

D

L

C

N

F

N

L
|
L

N

K

G
|
G

I
|
I

T
|
T

R

R

Q

Q

T

V

V

V

I

I

G

E

M

L

L

I

K

N

D

E

R

L

G

E

I

I

T

P

V

F

H

R

E

E

R

A

H

N

I

I

M

G

P

L

E

F

E

D

L

L

D

Y

G

S

F

A

E

D

Q

E

C

I

W

F

L

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

V

V

G

T

Q

Y

I

I

active site: F33 (= F39), G35 (= G41), K151 (= K157), E184 (= E191), L207 (= L214)
binding 2-oxoglutaric acid: F33 (= F39), G35 (= G41), Y88 (= Y94), R90 (= R96), K151 (= K157), G187 (= G194), G246 (= G253), T247 (= T254), A248 (= A255)
binding pyridoxal-5'-phosphate: R52 (= R58), K151 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), D188 (≠ A195), N189 (= N196), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
37% identity, 96% coverage: 11:286/288 of query aligns to 9:286/301 of 6thqB

query
sites
6thqB

I

V

W

W

M

L

D

D

G

G

E

R

M

L

V

V

N

D

W

E

R

E

D

E

A

A

K

K

V

V

H

T

I

V

L

L

S

S

H

P

A

S

M

L

H

N

Y
|
Y

A

G

S

F

S

G

V

V

F
|
F

E

E

G

G

E

I

R

R

A

A

Y

Y

-

W

N

N

G

G

K

E

-

N

-

L

-

Y

I

V

F

F

K

R

S

L

R

R

E

D

H

H

S

M

E

E

R
|
R

L

L

I

L

A

R

S

S

A

A

E

K

A

I

L

I

D

G

M

L

P

D

M

V

P

P

Y

Y

T

T

V

A

D

E

Q

E

I

L

E

S

A

K

A

A

K

V

E

V

E

E

T

T

L

V

K

R

A

A

S

N

G

G

L

F

-

K

Q

E

D

D

A

L

Y
|
Y

V

I

R

R

A

P

V

V

V

A

W

Y

R

I

G

-

S

S

G

K

E

P

D

Q

M
x
I

G

S

V

L

A

D

S

V

A

R

K

G

N

L

P

Q

V

A

R

S

M

V

A

A

I

I

A

A

V

A

W

I

G

P

W

F

G

G

A

K

Y

Y

Y

L

G

-

D

-

A

-

K

K

M

V

Q

E

G

G

A

V

K

R

L

A

D

A

I

V

A

V

E

S

W

W

K

R

R
|
R

P

V

S

H

P

T

E

S

T

M

I

M

P

P

V

V

H

M

A

A

K
|
K

A

A

A

T

G

G

L

I

Y
|
Y

M

L

I

N

C

S

T

I

I

M

S

A

K

A

H

V

K

E

A

A

E

R

A

A

K

R

G

G

C

Y

S

D

D

E

A

A

L

I

F

M

M

L

D

N

Y

A

R

E

G

G

Y

K

V

V

A

V

E
|
E

A

G

T
x
S

G
|
G

A
x
E

N
|
N

I

I

F

F

F

I

V

V

K

R

D

R

G

G

E

V

V

L

H

M

T

T

P

P

K

P

-

L

P

E

D

D

C

G

F

I

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

E

T

T

V

V

I

I

G

S

M

I

L

A

K

G

D

D

R

L

G

G

I

I

T

P

V

L

H

L

E

E

R

K

H

S

I

I

M

T

P

R

E

E

L

L

D

Y

G

A

F

A

E

D

Q

E

C

A

W

F

L

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

I

G

I

Q

E

I

I

G

D

P

G

H

R

S

V

F

L

E

Q

V

R

G

G

E

P

L

I

T

T

Q

Q

D

K

I

I

A

A

K

E

A

T

Y

Y

E

R

L

I

V

V

active site: F37 (= F39), K156 (= K157), E190 (= E191), L214 (= L214)
binding pyridoxal-5'-phosphate: R60 (= R58), R145 (= R146), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: Y32 (= Y34), R60 (= R58), Y97 (= Y94), I109 (≠ M107), K156 (= K157), Y161 (= Y162), E190 (= E191), S192 (≠ T193), G193 (= G194), E194 (≠ A195), N195 (= N196), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 2ej3A

query
sites
2ej3A

R

K

D

A

G

G

L

L

I

I

W

W

M

M

D

N

G

G

E

A

M

F

V

V

N

P

W

Q

R

E

D

E

A

A

K

K

V

T

H

S

I

V

L

L

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

K

R

S

L

R

K

E

E

H

H

S

V

E

K

R
|
R

L

F

I

Y

A

N

S

S

A

A

E

K

A

V

L

L

D

R

M

M

P

E

M

I

P

P

Y

F

T

A

V

P

D

E

Q

E

I

L

E

E

A

E

A

A

K

I

E

K

E

E

T

V

L

V

K

R

A

R

S

N

G

G

L

Y

Q

R

D

S

A

C

Y
|
Y

V

I

R
|
R

A

P

V

L

V

A

W

W

R

M

G

G

S

A

G

-

E

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

K

N

N

N

P

P

V

A

R

E

M

V

A

M

I

V

A

A

V

A

W

W

G

E

W

W

G

-

A

-

Y

-

G

-

D

-

A

-

K

V

M

R

Q

K

G

G

A

A

K

R

L

L

D

I

I

T

A

S

E

S

W

W

K

A

R
|
R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

I

L

S

A

K

K

H

M

K

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N

N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

K

K

D

D

R

L

G

G

I

Y

T

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

D

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G
|
G

T
|
T

A
|
A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
binding [1-(aminomethyl)cyclohexyl]acetic acid: Y94 (= Y94), R96 (= R96), Y155 (= Y162), G187 (= G194), E188 (≠ A195), G246 (= G253), T247 (= T254), A248 (= A255), A249 (= A256)
binding pyridoxal-5'-phosphate: R58 (= R58), R139 (= R146), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), E188 (≠ A195), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 2eiyA

query
sites
2eiyA

R

K

D

A

G

G

L

L

I

I

W

W

M

M

D

N

G

G

E

A

M

F

V

V

N

P

W

Q

R

E

D

E

A

A

K

K

V

T

H

S

I

V

L

L

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

K

R

S

L

R

K

E

E

H

H

S

V

E

K

R
|
R

L

F

I

Y

A

N

S

S

A

A

E

K

A

V

L

L

D

R

M

M

P

E

M

I

P

P

Y

F

T

A

V

P

D

E

Q

E

I

L

E

E

A

E

A

A

K

I

E

K

E

E

T

V

L

V

K

R

A

R

S

N

G

G

L

Y

Q

R

D

S

A

C

Y
|
Y

V

I

R

R

A

P

V

L

V

A

W

W

R

M

G

G

S

A

G

-

E

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

K

N

N

N

P

P

V

A

R

E

M

V

A

M

I

V

A

A

V

A

W

W

G

E

W

W

G

-

A

-

Y

-

G

-

D

-

A

-

K

V

M

R

Q

K

G

G

A

A

K

R

L

L

D

I

I

T

A

S

E

S

W

W

K

A

R
|
R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

I

L

S

A

K

K

H

M

K

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

K

K

D

D

R

L

G

G

I

Y

T

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

D

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
binding 4-methyl valeric acid: F35 (= F39), Y94 (= Y94), Y155 (= Y162), T247 (= T254), A248 (= A255)
binding pyridoxal-5'-phosphate: R58 (= R58), R139 (= R146), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), E188 (≠ A195), N189 (= N196), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
38% identity, 90% coverage: 7:264/288 of query aligns to 3:257/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

L

L

I

I

W

W

M

M

D

N

G

G

E

A

M

F

V

V

N

P

W

Q

R

E

D

E

A

A

K

K

V

T

H

S

I

V

L

L

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

K

R

S

L

R

K

E

E

H

H

S

V

E

K

R
|
R

L

F

I

Y

A

N

S

S

A

A

E

K

A

V

L

L

D

R

M

M

P

E

M

I

P

P

Y

F

T

A

V

P

D

E

Q

E

I

L

E

E

A

E

A

A

K

I

E

K

E

E

T

V

L

V

K

R

A

R

S

N

G

G

L

Y

Q

R

D

S

A

C

Y

Y

V

I

R

R

A

P

V

L

V

A

W

W

R

M

G

G

S

A

G

-

E

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

K

N

N

N

P

P

V

A

R

E

M

V

A

M

I

V

A

A

V

A

W

W

G

E

W

W

G

-

A

-

Y

-

G

-

D

-

A

-

K

V

M

R

Q

K

G

G

A

A

K

R

L

L

D

I

I

T

A

S

E

S

W

W

K

A

R
|
R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

I

L

S

A

K

K

H

M

K

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

K

K

D

D

R

L

G

G

I

Y

T

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

D

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G
|
G

T
|
T

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F39), G37 (= G41), K150 (= K157), E184 (= E191), L207 (= L214)
binding pyridoxal-5'-phosphate: R58 (= R58), R139 (= R146), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), E188 (≠ A195), N189 (= N196), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
38% identity, 90% coverage: 7:264/288 of query aligns to 3:254/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

L

L

I

I

W

W

M

M

D

N

G

G

E

A

M

F

V

V

N

P

W

Q

R

E

D

E

A

A

K

K

V

T

H

S

I

V

L

L

S

S

H

H

A

A

M

L

H

H

Y

Y

A

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

A

A

Y

Y

N

E

G

T

K

A

-

K

-

G

-

P

-

A

I

I

F

F

K

R

S

L

R

K

E

E

H

H

S

V

E

K

R
|
R

L

F

I

Y

A

N

S

S

A

A

E

K

A

V

L

L

D

R

M

M

P

E

M

I

P

P

Y

F

T

A

V

P

D

E

Q

E

I

L

E

E

A

E

A

A

K

I

E

K

E

E

T

V

L

V

K

R

A

R

S

N

G

G

L

Y

Q

R

D

S

A

C

Y
|
Y

V

I

R

R

A

P

V

L

V

A

W

W

R

M

G

G

S

A

G

-

E

K

D

A

M

L

G

G

V

V

A

N

S

P

-

L

A

P

K

N

N

N

P

P

V

A

R

E

M

V

A

M

I

V

A

A

V

A

W

W

G

E

W

W

G

G

A

A

Y

-

G

-

D

-

A

-

K

-

M

-

Q

-

G

-

A

-

K

R

L

L

D

I

I

T

A

S

E

S

W

W

K

A

R
|
R

P

F

S

P

P

A

E

N

T

V

I

M

P

P

V

G

H

K

A

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

I

L

S

A

K

K

H

M

K

E

A

A

E

V

A

A

K

A

G

G

C

A

S

D

D

E

A

A

L

L

F

L

M

L

D

D

Y

E

R

E

G

G

Y

Y

V

V

A

A

E
|
E

A

G

T
x
S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

K

R

D

D

G

G

E

V

V

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

G

R

M

I

L

A

K

K

D

D

R

L

G

G

I

Y

T

E

V

V

H

Q

E

V

R

V

H

R

I

A

M

T

P

R

E

D

E

Q

L

L

D

Y

G

M

F

A

E

D

Q

E

C

V

W

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F39), G37 (= G41), K147 (= K157), E181 (= E191), L204 (= L214)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: G37 (= G41), R58 (= R58), Y94 (= Y94), R136 (= R146), K147 (= K157), Y152 (= Y162), E181 (= E191), S183 (≠ T193), G184 (= G194), E185 (≠ A195), N186 (= N196), L204 (= L214), G206 (= G216), I207 (= I217), T208 (= T218), G243 (= G253), T244 (= T254), A245 (= A255)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
27% identity, 96% coverage: 11:286/288 of query aligns to 6:290/307 of 6q8eA

query
sites
6q8eA

I

L

W

W

M

W

D

N

G

H

E

R

M

I

V

I

N

P

W

W

R

E

D

E

A

A

K

T

V

V

H

H

I

L

L

T

S

D

H

Y

A

W

M

W

H

A

Y

S

A

V

S

T

S

A

V

V

F
|
F

E

E

G

G

E

I

R

R

A

G

Y

Y

N

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

I

I

F

F

K

R

S

L

R

E

E

D

H

H

S

A

E

R

R
|
R

L

L

I

E

A

Q

S

S

A

M

E

Q

A

L

L

I

D

R

M

M

P

P

M

K

P

E

Y

F

T

T

V

V

D

D

Q

E

I

I

E

C

A

Q

A

A

K

T

E

I

E

D

T

L

L

V

K

R

A

A

S

N

G

D

L

Y

Q

R

-

G

D

D

A

V

Y

Y

V

I

R

M

A

P

V

L

V

A

W

Y

R

A

G

V

S

G

G

N

E

K

D

A

M

F

G

S

V

V

A

V

S

G

A

D

K

R

N

T

P

T

V

E

R

M

M

F

A

I

I

Y

A

S

V

R

W

P

G

A

W

V

G

S

A

R

Y

L

Y

E

G

E

D

D

A

F

K

S

M

L

Q

H

G

A

A

C

K

-

L

-

D

-

I

Y

A

S

E

S

W

W

K

T

R

R

P

I

S

N

P

E

E

R

T

V

I

L

P

P

V

P

H

R

A

I

K
|
K

A

A

A

L

G

A

L

N

Y
|
Y

M

R

I

N

C

S

T

Q

I

L

S

A

K

S

H

S

K

E

A

A

E

A

A

M

K

N

G

G

C

Y

S

D

D

T

A

A

L

L

F

F

M

L

D

N

Y

P

R

E

G

G

Y

K

V

V

A

A

E
|
E

A

G

T
|
T

G
|
G

A
x
S

N
x
C

I

V

F

F

V

V

K

R

D

K

G

G

E

K

V

L

H

I

T

T

P

P

K

D

-

I

P

T

D

S

C

G

F

I

L
|
L

N

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

T

T

V

V

I

I

G

H

M

L

L

A

K

R

D

E

-

V

R

L

G

G

I

L

T

E

V

V

H

E

E

E

R

R

H

V

I

V

M

D

P

R

E

T

E

E

L

T

D

Y

G

L

F

A

E

D

Q

E

C

A

W

F

L

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

V

I

G

A

Q

S

I

I

G

D

P

R

H

H

S

E

F

M

E

K

-

H

-

G

-

A

V

P

G

G

E

P

L

I

T

T

Q

R

D

Q

I

L

A

R

K

D

A

I

Y

Y

E

R

E

E

L

V

V

V

active site: F34 (= F39), K156 (= K157), E190 (= E191), L214 (= L214)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), T192 (= T193), G193 (= G194), S194 (≠ A195), C195 (≠ N196), L214 (= L214), S216 (≠ G216), I217 (= I217), T218 (= T218), G254 (= G253), T255 (= T254)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
26% identity, 96% coverage: 11:286/288 of query aligns to 6:290/309 of 7neaA

query
sites
7neaA

I

L

W

W

M

W

D

N

G

H

E

R

M

I

V

I

N

P

W

W

R

E

D

E

A

A

K

T

V

V

H

H

I

L

L

T

S

D

H

Y

A

W

M

W

H

A

Y

S

A

V

S

T

S

A

V

V

F
|
F

E

E

G

V

E

I

R

S

A

G

Y

Y

N

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

I

I

F

F

K

R

S

L

R

E

E

D

H

H

S

A

E

R

R
|
R

L

L

I

E

A

Q

S

S

A

M

E

Q

A

L

L

I

D

R

M

M

P

P

M

K

P

E

Y

F

T

T

V

V

D

D

Q

E

I

I

E

C

A

Q

A

A

K

T

E

I

E

D

T

L

L

V

K

R

A

A

S

N

G

D

L

Y

Q

R

-

G

D

D

A

V

Y

F

V

I

R

M

A

P

V

L

V

A

W

Y

R

A

G

V

S

G

G

N

E

K

D

A

M

F

G

S

V

V

A

V

S

G

A

D

K

R

N

T

P

T

V

E

R

M

M

F

A

I

I

Y

A

S

V

R

W

P

G

A

W

V

G

S

A

R

Y

L

Y

E

G

E

D

D

A

F

K

S

M

L

Q

H

G

A

A

C

K

-

L

-

D

-

I

Y

A

S

E

S

W

W

K

T

R

R

P

I

S

N

P

E

E

R

T

V

I

L

P

P

V

P

H

R

A

I

K
|
K

A

A

A

L

G

A

L

N

Y
|
Y

M

R

I

N

C

S

T

Q

I

L

S

A

K

S

H

S

K

E

A

A

E

A

A

M

K

N

G

G

C

Y

S

D

D

T

A

A

L

L

F

F

M

L

D

N

Y

P

R

E

G

G

Y

K

V

V

A

A

E
|
E

A

G

T

T

G
|
G

A
x
S

N
x
C

I

V

F

F

V

V

K

R

D

K

G

G

E

K

V

L

H

I

T

T

P

P

K

D

P

I

D

T

C

S

-

G

F

I

L
|
L

N

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

T

T

V

V

I

I

G

H

M

L

L

A

K

R

D

E

-

V

R

L

G

G

I

L

T

E

V

V

H

E

E

E

R

R

H

V

I

V

M

D

P

R

E

T

E

E

L

T

D

Y

G

L

F

A

E

D

Q

E

C

A

W

F

L

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

V

I

G

A

Q

S

I

I

G

D

P

R

H

H

S

E

F

M

E

K

-

H

-

G

-

A

V

P