SitesBLAST
Comparing GFF3390 FitnessBrowser__Phaeo:GFF3390 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 96% coverage: 16:375/375 of query aligns to 16:374/378 of P69874
- C26 (≠ R26) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F27) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F45) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C54) mutation to T: Loss of ATPase activity and transport.
- L60 (≠ M60) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L76) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ T135) mutation to M: Loss of ATPase activity and transport.
- D172 (= D172) mutation to N: Loss of ATPase activity and transport.
- C276 (≠ L277) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E298) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
43% identity, 84% coverage: 18:332/375 of query aligns to 7:331/375 of 2d62A
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 3:321/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 3:321/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F27), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), Q81 (= Q96), R128 (= R143), A132 (≠ Q147), S134 (= S149), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 3:321/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F27), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), S134 (= S149), Q137 (= Q152)
- binding beryllium trifluoride ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G136 (= G151), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 3:321/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (≠ A32), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), S134 (= S149), Q137 (= Q152)
- binding tetrafluoroaluminate ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G135 (= G150), G136 (= G151), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 3:321/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (≠ A32), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), S134 (= S149), Q137 (= Q152)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 85% coverage: 18:336/375 of query aligns to 4:322/371 of P68187
- A85 (= A99) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V128) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ M131) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ R133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ L242) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ I253) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (vs. gap) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G288) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ H292) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ A294) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G316) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ A322) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (= S336) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 1:319/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F27), S35 (= S52), G36 (= G53), C37 (= C54), G38 (= G55), K39 (= K56), S40 (≠ T57), T41 (= T58), R126 (= R143), A130 (≠ Q147), S132 (= S149), G134 (= G151), Q135 (= Q152)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 85% coverage: 18:336/375 of query aligns to 4:320/369 of P19566
- L86 (= L100) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ A322) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1g291 Malk (see paper)
40% identity, 86% coverage: 18:339/375 of query aligns to 4:333/372 of 1g291
- binding magnesium ion: D69 (≠ P83), E71 (vs. gap), K72 (vs. gap), K79 (≠ N87), D80 (≠ K88), E292 (= E298), D293 (≠ K299)
- binding pyrophosphate 2-: S38 (= S52), G39 (= G53), C40 (= C54), G41 (= G55), K42 (= K56), T43 (= T57), T44 (= T58)
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
40% identity, 85% coverage: 18:334/375 of query aligns to 7:311/353 of 1vciA
8hplC Lpqy-sugabc in state 1 (see paper)
44% identity, 65% coverage: 18:262/375 of query aligns to 3:248/384 of 8hplC
8hprC Lpqy-sugabc in state 4 (see paper)
44% identity, 65% coverage: 18:262/375 of query aligns to 3:250/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F27), S38 (= S52), G39 (= G53), G41 (= G55), K42 (= K56), S43 (≠ T57), Q82 (= Q96), Q133 (= Q147), G136 (= G150), G137 (= G151), Q138 (= Q152), H192 (= H206)
- binding magnesium ion: S43 (≠ T57), Q82 (= Q96)
8hprD Lpqy-sugabc in state 4 (see paper)
44% identity, 65% coverage: 18:262/375 of query aligns to 3:250/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F27), S38 (= S52), C40 (= C54), G41 (= G55), K42 (= K56), S43 (≠ T57), T44 (= T58), Q82 (= Q96), R129 (= R143), Q133 (= Q147), S135 (= S149), G136 (= G150), G137 (= G151), Q159 (≠ E173), H192 (= H206)
- binding magnesium ion: S43 (≠ T57), Q82 (= Q96)
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 62% coverage: 18:249/375 of query aligns to 4:236/393 of P9WQI3
- H193 (= H206) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 77% coverage: 18:305/375 of query aligns to 4:286/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 77% coverage: 18:305/375 of query aligns to 4:286/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 77% coverage: 18:305/375 of query aligns to 4:286/353 of 1oxuA
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
37% identity, 77% coverage: 18:305/375 of query aligns to 4:286/353 of Q97UY8
- S142 (= S149) mutation to A: Decrease in ATPase activity. Can form dimers.
- G144 (= G151) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
- E166 (= E173) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).
Query Sequence
>GFF3390 FitnessBrowser__Phaeo:GFF3390
MTIPVFEPWNDPEAKPLIQFQNVTKRFGEFTAIDDLTLGIYEKEFFALLGPSGCGKTTMM
RMLAGFETPTEGKIFLSGQDIAPVPPNKRLVNMMFQSYALFPHLSVWDNIAFGLKRENKP
KHDIAERVQEMLRLTRLEKFARRKPHQISGGQRQRVALARSLAKAPKLLLLDEPLGALDK
KLRQDTQFELMDIQEKTGTTFVIVTHDQEEAMTVASRVAVMDNGRIVQVATPDRIYETPN
SLYVADFIGDVNIIGGTATPTGPEQYAVNWKDGAAPLTVKSQASFSDGQECHLAIRPEKV
TISAERPAEADNTVQGRILDIAYLGNISTYHVELPSGAVIKAQAANTRRIARRAFTWEDP
VWLSWTATAGVLLAN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory