SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3392 FitnessBrowser__Phaeo:GFF3392 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
31% identity, 54% coverage: 62:206/271 of query aligns to 86:234/296 of P68183

query
sites
P68183

W

W

V

N

T

S

L

V

K

K

V

V

A

A

V

G

M

I

S

S

S

A

T

I

I

G

A

I

T

V

V

A

L

L

G

S

T

T

M

T

A

C

A

A

Y

Y

V

A

L

F

V

A

R

R

G

M

G

-

R

R

F

F

L

P

G

G

R

K

-

A

T

T

L

L

F

L

S

K

G

G

M

M

I

L

Y

I

A

F

P

Q

L

M

V

-

M

F

P

P

E

A

V

V

I

L

T

S

G

L

L

V

S

A

L

L

L

Y

L

A

L

L

F

F

-

D

-

R

-

L

-

G

-

E

-

Y

-

I

-

P

-

F

I

I

G

G

I

L

G

N

L

T

D

H

R

G

G

G

V

V

M

I

T

F

I

A

V

Y

L

L

A

G

H

G

T

I

T

A

F

L

S

H

M

V

C

W

Y

-

V

-

S

-

V

-

V

T

V

I

S

K

S

G

R

Y

L

F

V

E

T

T

F

I

D

D

R

S

S

S

L

L

E

E

E
|
E

A

A

A
|
A

L

A

D

L

L

D

G
|
G

C

A

S

T

A

P

A

W

E

Q

A

A

F

F

R

R

L

L

V

V

T

L

L

L

P
|
P

I

L

I

S

A

V

P

P

A

I

V

L

I

A

S

V

G

V

W

F

L

I

L

L

A

S

F

F

T

I

L

A

S

A

L

I

D

T

D

E

L

V

V

P

I

V

A

A

S

S

E190 (= E162) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A164) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G168) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P181) mutation to A: No effect.

Query Sequence

>GFF3392 FitnessBrowser__Phaeo:GFF3392
MTRLSWFNTVSLTLGFAFLYIPMVILIIFSFNESKLVTVWAGFSVKWYGELLQNEAFLNA
AWVTLKVAVMSSTIATVLGTMAAYVLVRGGRFLGRTLFSGMIYAPLVMPEVITGLSLLLL
FIGIGLDRGVMTIVLAHTTFSMCYVSVVVSSRLVTFDRSLEEAALDLGCSAAEAFRLVTL
PIIAPAVISGWLLAFTLSLDDLVIASFTSGPAATTLPIKIFSAVRLGVSPEINALSTIMI
AIVTLGVVTASLVTKRQLVRQRRDEQNAERS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory