SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3407 FitnessBrowser__Marino:GFF3407 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTK9 Rubredoxin-NAD(+) reductase; RdxR; EC 1.18.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
59% identity, 99% coverage: 1:380/384 of query aligns to 1:380/384 of Q9HTK9

query
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Q9HTK9

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TGLA 12:15 (≠ TGLS 12:15) binding FAD
TA 36:37 (= TA 36:37) binding FAD
K45 (= K45) binding FAD
V83 (= V83) binding FAD
E159 (= E159) binding FAD
D277 (= D277) binding FAD
V289 (= V289) binding FAD
K320 (= K320) binding FAD

2v3aA Crystal structure of rubredoxin reductase from pseudomonas aeruginosa. (see paper)
59% identity, 98% coverage: 4:380/384 of query aligns to 1:377/381 of 2v3aA

query
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2v3aA

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active site: L11 (= L14), K42 (= K45), P43 (= P46), M290 (= M293)
binding flavin-adenine dinucleotide: I7 (≠ V10), G8 (= G11), T9 (= T12), G10 (= G13), A12 (≠ S15), T33 (= T36), A34 (= A37), D35 (= D38), K42 (= K45), P43 (= P46), R79 (≠ T82), V80 (= V83), A105 (= A108), W106 (= W109), G107 (= G110), I153 (= I156), F157 (= F160), D274 (= D277), L284 (= L287), Y285 (= Y288), V286 (= V289), K317 (= K320)

3fg2P Crystal structure of ferredoxin reductase for the cyp199a2 system from rhodopseudomonas palustris (see paper)
32% identity, 71% coverage: 7:279/384 of query aligns to 4:277/404 of 3fg2P

query
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3fg2P

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binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), H11 (≠ L14), A12 (≠ S15), D34 (≠ A37), E35 (≠ D38), R42 (≠ K45), P43 (= P46), S46 (= S49), K47 (≠ T50), R78 (≠ T82), M79 (≠ V83), T106 (≠ W109), R127 (≠ N130), I153 (= I156), D275 (= D277)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 292, 293, 321

6tukB Crystal structure of fdr9 (see paper)
33% identity, 71% coverage: 7:279/384 of query aligns to 4:262/393 of 6tukB

query
sites
6tukB

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A

L

P

L

L

P

S

E

A

A

A

A

L

A

G

H

A

A

D

V

V

Q

G

Q

Q

E

R

L

F

E

L

N

P

L

W

G

W

-

S

-

E

V

V

G

D

F

L

H

R

L

L

G

D

T

T

V

G

V

V

D

A

R

E

I

V

D

T

R

E

R

T

G

G

D

-

G

-

V

-

S

-

L

V

T

Q

L

L

A

A

N

N

G

G

E

E

T

Q

L

V

D

D

A

A

D

D

L

V

V

V

L

V

S

T

A

G

V

I

G

G

L

V

R

R

P

P

R

A

T

V

D

D

L

W

A

L

R

S

A

G

A

S

G

G

L

I

E

A

T

L

A

D

R

T

G

G

I

V

V

V

N

D

R

E

A

H

L

L

E

R

T

T

S

S

A

L

P

P

D

G

V

I

Y

Y

A

A

L

L

G
|
G

D
|
D

C

V

A

A

binding flavin-adenine dinucleotide: V7 (= V10), G8 (= G11), G9 (≠ T12), G10 (= G13), A12 (≠ S15), A34 (= A37), E35 (≠ D38), R42 (≠ K45), P43 (= P46), K47 (≠ T50), A75 (≠ T82), A76 (≠ V83), T102 (≠ W109), G103 (= G110), V118 (≠ I129), R119 (≠ N130), G259 (= G276), D260 (= D277)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 277, 278, 311, 312

1xhcA Nadh oxidase /nitrite reductase from pyrococcus furiosus pfu-1140779- 001
28% identity, 90% coverage: 5:348/384 of query aligns to 1:328/346 of 1xhcA

query
sites
1xhcA

A

S

P

K

I

V

I

I

V
|
V

G
|
G

T

N

G
|
G

L
x
P

S
x
G

G

G

Y

F

S

E

L

L

A

A

R

K

E

Q

I

L

R

-

K

-

Q

-

D

S

K

Q

E

T

T

Y

P

E

V

V

L

T

I

V

V

I

T
x
D

A
x
K

D

E

D

P

G

V

V

P

S

Y

Y

Y

S

S

K
|
K

P
|
P

M

M

L

L

S

S

T

H

G

Y

F

I

T

A

K

G

G

F

K

I

D

P

A

R

D

N

G

R

L

L

A

F

Q

P

G

Y

G

S

T

L

D

D

A

W

M

Y

A

R

D

K

Q

R

L

-

N

G

V

I

R

E

I

I

R

R

T

L

F

A

V

E

T
x
E

V
x
A

T

K

G

L

I

I

D

D

T

R

E

G

A

R

H

K

E

V

L

V

I

I

L

T

G

E

D

K

E

G

R

E

L

V

A

P

Y

Y

S

D

K

T

L

L

V

V

L

L

A
|
A

W
x
T

G
|
G

A

A

D

R

V

A

I

R

R

E

L

P

G

Q

L

I

D

K

G

G

D

-

G

-

Q

K

E

E

H

Y

V

L

F

L

S

T

I
x
L

N
x
R

D

T

L

I

M

F

D

D

Y

A

R

D

A

R

F

I

R

K

D

E

A

S

L

I

G

E

D

N

G

S

K

G

R

E

V

A

A

I

I

I

M

I

G

G

A

G

G

G

L
x
F

I
|
I

G

G

C

L

E
|
E

F

L

A

A

N

G

D

N

L

L

R

A

N

E

G

A

G

G

Y

Y

E

H

V

V

D

K

V

L

I

I

A

H

P

R

S

G

E

A

E

M

V

F

M

L

P

G

G

-

L

-

L

L

P

D

E

E

A

E

A

L

A

S

H

N

A

M

V

I

Q

K

Q

D

E

M

L

L

E

E

N

E

L

T

G

G

V

V

G

K

F

F

H

F

L

L

G

N

T

S

V

E

V

L

D

L

R

E

I

A

D

N

R

E

G

G

D

-

G

-

V

-

S

-

L

-

T

V

L

L

A

T

N

N

G

S

E

G

T

F

L

I

D

E

A

G

D

K

L

V

V

K

L

I

S

C

A

A

V

I

G

G

L

I

R

V

P

P

R

N

T

V

D

D

L

L

A

A

R

R

A

R

A

S

G

G

L

I

E

H

T

T

A

G

R

R

G

G

I

I

V

L

V

I

N

D

R

D

A

N

L

F

E

R

T

T

S

S

A

A

P

K

D

D

V

V

Y

Y

A

A

L

I

G
|
G

D
|
D

C

C

A

A

E

E

V

Y

D

S

G

G

H

I

V

I

L

A

L
x
G

Y
x
T

V
x
A

L

K

P

A

L

A

M
|
M

A

E

C

Q

S

A

R

R

A

V

L

L

A

A

K

D

T

I

L

L

L

K

G

G

E

E

R

P

T

R

E

R

V

Y

S

N

Y

F

G

K

T

F

M

R

P

S

V

T

M

V

I

F

K

K

T

F

P

G

C

K

C

L

P

Q

T

I

A

A

V

I

C

I

P

-

P

G

P

N

S

T

E

K

A

G

E

E

G

G

N

K

W

W

E

-

I

-

E

I

Q

E

D

D

G

N

T

T

S

K

V

V

active site: P10 (≠ L14), K38 (= K45), P39 (= P46), F145 (≠ L155), E149 (= E159), M276 (= M293)
binding flavin-adenine dinucleotide: V6 (= V10), G7 (= G11), G9 (= G13), P10 (≠ L14), G11 (≠ S15), D29 (≠ T36), K30 (≠ A37), K38 (= K45), P39 (= P46), E74 (≠ T82), A75 (≠ V83), A100 (= A108), T101 (≠ W109), G102 (= G110), L119 (≠ I129), R120 (≠ N130), F145 (≠ L155), I146 (= I156), E149 (= E159), G259 (= G276), D260 (= D277), G270 (≠ L287), T271 (≠ Y288), A272 (≠ V289)

Q8U1K9 NAD(P)H:rubredoxin oxidoreductase; NROR; Rubredoxin--NAD(P)(+) reductase; EC 1.18.1.4 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
28% identity, 89% coverage: 7:348/384 of query aligns to 3:328/359 of Q8U1K9

query
sites
Q8U1K9

I

V

I

I

V

V

G

G

T

N

G

G

L

P

S
x
G

G

G

Y

F

S

E

L

L

A

A

R

K

E

Q

I

L

R

-

K

-

Q

-

D

S

K

Q

E

T

T

Y

P

E

V

V

L

T

I

V

V

I

T
x
D

A
x
K

D

E

D

P

G

V

V

P

S

Y

Y

Y

S

S

K
|
K

P
|
P

M

M

L

L

S

S

T

H

G

Y

F

I

T

A

K

G

G

F

K

I

D

P

A

R

D

N

G

R

L

L

A

F

Q

P

G

Y

G

S

T

L

D

D

A

W

M

Y

A

R

D

K

Q

R

L

-

N

G

V

I

R

E

I

I

R

R

T

L

F

A

V

E

T

E

V
x
A

T

K

G

L

I

I

D

D

T

R

E

G

A

R

H

K

E

V

L

V

I

I

L

T

G

E

D

K

E

G

R

E

L

V

A

P

Y

Y

S

D

K

T

L

L

V

V

L

L

A

A

W

T

G

G

A
|
A

D

R

V

A

I

R

R

E

L

P

G

Q

L

I

D

K

G

G

D

-

G

-

Q

K

E

E

H

Y

V

L

F

L

S

T

I

L

N
x
R

D

T

L

I

M

F

D

D

Y

A

R

D

A

R

F

I

R

K

D

E

A

S

L

I

G

E

D

N

G

S

K

G

R

E

V

A

A

I

I

I

M

I

G

G

A

G

G

G

L

F

I

I

G

G

C

L

E

E

F

L

A

A

N

G

D

N

L

L

R

A

N

E

G

A

G

G

Y

Y

E

H

V

V

D

K

V

L

I

I

A

H

P

R

S

G

E

A

E

M

V

F

M

L

P

G

G

-

L

-

L

L

P

D

E

E

A

E

A

L

A

S

H

N

A

M

V

I

Q

K

Q

D

E

M

L

L

E

E

N

E

L

T

G

G

V

V

G

K

F

F

H

F

L

L

G

N

T

S

V

E

V

L

D

L

R

E

I

A

D

N

R

E

G

G

D

-

G

-

V

-

S

-

L

-

T

V

L

L

A

T

N

N

G

S

E

G

T

F

L

I

D

E

A

G

D

K

L

V

V

K

L

I

S

C

A

A

V

I

G

G

L

I

R

V

P

P

R

N

T

V

D

D

L

L

A

A

R

R

A

R

A

S

G

G

L

I

E

H

T

T

A

G

R

R

G

G

I

I

V

L

V

I

N

D

R

D

A

N

L

F

E

R

T

T

S

S

A

A

P

K

D

D

V

V

Y

Y

A

A

L

I

G

G

D
|
D

C

C

A

A

E

E

V

Y

D

S

G

G

H

I

V

I

L

A

L

G

Y

T

V

A

L

K

P

A

L

A

M

M

A

E

C

Q

S

A

R

R

A

V

L

L

A

A

K

D

T

I

L

L

L

K

G

G

E

E

R

P

T

R

E

R

V

Y

S

N

Y

F

G

K

T

F

M

R

P

S

V

T

M

V

I

F

K

K

T

F

P

G

C

K

C

L

P

Q

T

I

A

A

V

I

C

I

P

-

P

G

P

N

S

T

E

K

A

G

E

E

G

G

N

K

W

W

E

-

I

-

E

I

Q

E

D

D

G

N

T

T

S

K

V

V

G11 (≠ S15) binding FAD
DK 29:30 (≠ TA 36:37) binding FAD
KP 38:39 (= KP 45:46) binding FAD
A75 (≠ V83) binding FAD
A103 (= A111) binding FAD
R120 (≠ N130) binding FAD
D260 (= D277) binding FAD

P16640 Putidaredoxin reductase CamA; Pdr; Putidaredoxin--NAD(+) reductase; EC 1.18.1.5 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
31% identity, 75% coverage: 1:288/384 of query aligns to 1:293/422 of P16640

query
sites
P16640

M

M

T

N

E

A

Q

N

A

D

P

N

I

V

I

I

V

V

G

G

T

T

G

G

L

L

S
x
A

G

G

Y

V

S

E

L

V

A

A

R

F

E

G

I

L

R

R

K

A

Q

S

D

G

K

W

E

E

T

G

P

N

V

I

L

R

I

L

V

V

T

G

A
x
D

D

A

D

T

G

V

V

I

S

P

Y

H

S

H

K

L

P

P

M

P

L

L

S

S

T
x
K

G

A

F

Y

T

L

K

A

G

G

K

K

-

A

D

T

A

A

D

E

G

S

L

L

A

Y

Q

L

G

R

G

T

T

P

D

D

A

A

M

Y

A

A

D

A

Q

Q

L

-

N

N

V

I

R

Q

I

L

R

L

T

G

F

G

V

T

T

Q

V
|
V

T

T

G

A

I

I

D

N

T

R

E

D

A

R

H

Q

E

Q

L

V

I

I

L

L

G

S

D

D

E

G

R

R

-

A

L

L

A

D

Y

Y

S

D

K

R

L

L

V

V

L

L

A

A

W

T

G

G

A

G

D

R

V

P

I

R

R

P

L

L

G

P

L

V

D

A

G

S

D

G

-

A

-

V

G

G

Q

K

E

A

H

N

V

N

F

F

S

R

-

Y

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

E

A

C

F

I

R

R

D

R

A

Q

L

L

G

I

D

A

G

D

K

N

R

R

V

L

A

V

I

V

M

I

G

G

A

G

G

G

L

Y

I

I

G

G

C

L

E

E

F

V

A

A

N

A

D

T

L

A

R

I

N

K

G

A

G

N

Y

M

E

H

V

V

D

T

V

L

I

L

A

D

P

T

S

A

E

A

E

R

V

V

M

L

P

E

G

R

L

V

L

T

P

A

E

P

A

P

A

V

A

S

H

A

A

F

V

Y

Q

E

Q

H

E

L

L

H

E

R

N

E

L

A

G

G

V

V

G

D

F

I

H

R

L

T

G

G

T

T

V

Q

V

V

D

C

R

G

I

F

D

E

R

M

R

S

G

T

D

D

G

Q

V

Q

S

K

L

V

T

T

L

A

A

V

-

L

-

C

-

E

N

D

G

G

E

T

T

R

L

L

D

P

A

A

D

D

L

L

V

V

L

I

S

A

A

G

V

I

G

G

L

L

R

I

P

P

R

N

T

C

D

E

L

L

A

A

R

S

A

A

G

G

L

L

E

Q

T

V

A

D

R

N

G

G

I

I

V

V

V

I

N

N

R

E

A

H

L

M

E

Q

T

T

S

S

A

D

P

P

D

L

V

I

Y

M

A

A

L

V

G

G

D
|
D

C

C

A

A

E

R

V

F

D

-

G

-

H

H

V

S

L

Q

L

L

Y

Y

A15 (≠ S15) binding FAD
D37 (≠ A37) binding FAD
K50 (≠ T50) binding FAD
V83 (= V83) binding FAD
R134 (≠ N130) binding FAD
D284 (= D277) binding FAD

Sites not aligning to the query:

302 binding FAD

1q1wA Crystal structure of putidaredoxin reductase from pseudomonas putida (see paper)
31% identity, 73% coverage: 7:288/384 of query aligns to 6:292/422 of 1q1wA

query
sites
1q1wA

I

V

I

I

V

V

G
|
G

T

T

G
|
G

L
|
L

S
x
A

G

G

Y

V

S

E

L

V

A

A

R

F

E

G

I

L

R

R

K

A

Q

S

D

G

K

W

E

E

T

G

P

N

V

I

L

R

I

L

V

V

T
x
G

A
x
D

D

A

D

T

G

V

V

I

S

P

Y

H

S

H

K
x
L

P
|
P

M

P

L

L

S

S

T
x
K

G

A

F

Y

T

L

K

A

G

G

K

K

-

A

D

T

A

A

D

E

G

S

L

L

A

Y

Q

L

G

R

G

T

T

P

D

D

A

A

M

Y

A

A

D

A

Q

Q

L

-

N

N

V

I

R

Q

I

L

R

L

T

G

F

G

V

T

T

Q

V
|
V

T

T

G

A

I

I

D

N

T

R

E

D

A

R

H

Q

E

Q

L

V

I

I

L

L

G

S

D

D

E

G

R

R

-

A

L

L

A

D

Y

Y

S

D

K

R

L

L

V

V

L

L

A
|
A

W
x
T

G
|
G

A

G

D

R

V

P

I

R

R

P

L

L

G

P

L

V

D

A

G

S

D

G

-

A

-

V

G

G

Q

K

E

A

H

N

V

N

F

F

S

R

-

Y

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

E

A

C

F

I

R

R

D

R

A

Q

L

L

G

I

D

A

G

D

K

N

R

R

V

L

A

V

I

V

M

I

G

G

A

G

G

G

L

Y

I
|
I

G

G

C

L

E

E

F

V

A

A

N

A

D

T

L

A

R

I

N

K

G

A

G

N

Y

M

E

H

V

V

D

T

V

L

I

L

A

D

P

T

S

A

E

A

E

R

V

V

M

L

P

E

G

R

L

V

L

T

P

A

E

P

A

P

A

V

A

S

H

A

A

F

V

Y

Q

E

Q

H

E

L

L

H

E

R

N

E

L

A

G

G

V

V

G

D

F

I

H

R

L

T

G

G

T

T

V

Q

V

V

D

C

R

G

I

F

D

E

R

M

R

S

G

T

D

D

G

Q

V

Q

S

K

L

V

T

T

L

A

A

V

-

L

-

C

-

E

N

D

G

G

E

T

T

R

L

L

D

P

A

A

D

D

L

L

V

V

L

I

S

A

A

G

V

I

G

G

L

L

R

I

P

P

R

N

T

C

D

E

L

L

A

A

R

S

A

A

G

G

L

L

E

Q

T

V

A

D

R

N

G

G

I

I

V

V

V

I

N

N

R

E

A

H

L

M

E

Q

T

T

S

S

A

D

P

P

D

L

V

I

Y

M

A

A

L

V

G

G

D
|
D

C

C

A

A

E

R

V

F

D

-

G

-

H

H

V

S

L

Q

L

L

Y

Y

active site: L13 (= L14), L44 (≠ K45), P45 (= P46)
binding flavin-adenine dinucleotide: G10 (= G11), G12 (= G13), L13 (= L14), A14 (≠ S15), G35 (≠ T36), D36 (≠ A37), L44 (≠ K45), P45 (= P46), K49 (≠ T50), V82 (= V83), A108 (= A108), T109 (≠ W109), G110 (= G110), R133 (≠ N130), I159 (= I156), D283 (= D277)

Sites not aligning to the query:

active site: 305
binding flavin-adenine dinucleotide: 300, 301, 329

1f3pA Ferredoxin reductase (bpha4)-nadh complex (see paper)
33% identity, 71% coverage: 4:277/384 of query aligns to 3:269/401 of 1f3pA

query
sites
1f3pA

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G

G

L
|
L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V
|
V

T

G

A
x
D

D
x
E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T

K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M
x
V

G
|
G

A

G

G
|
G

L
x
V

I
|
I

G

G

C

L

E
|
E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

-

P

-

S

-

E

-

E
|
E

V
x
T

M

Q

P

P

G

R

L

L

L

M

P

S

E
x
R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L
x
V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L13 (= L14), R44 (≠ K45), P45 (= P46)
binding flavin-adenine dinucleotide: A14 (≠ S15), V34 (= V35), D36 (≠ A37), E37 (≠ D38), R44 (≠ K45), P45 (= P46), A78 (≠ V83), T105 (≠ W109), G106 (= G110), R126 (≠ N130), G268 (= G276), D269 (= D277)
binding nicotinamide-adenine-dinucleotide: V147 (≠ M151), G148 (= G152), G150 (= G154), V151 (≠ L155), I152 (= I156), E155 (= E159), E171 (= E179), T172 (≠ V180), R179 (≠ E187), G230 (≠ A238), I231 (≠ V239), G232 (= G240), V233 (≠ L241)

Sites not aligning to the query:

active site: 291
binding flavin-adenine dinucleotide: 285, 286, 287, 290, 316
binding nicotinamide-adenine-dinucleotide: 285, 316, 317

8pxkA Structure of nadh-dependent ferredoxin reductase, bpha4, solved at wavelength 5.76 a (see paper)
33% identity, 71% coverage: 4:277/384 of query aligns to 4:270/403 of 8pxkA

query
sites
8pxkA

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G
|
G

L

L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T

G

A
x
D

D
x
E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T
x
K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M

V

G

G

A

G

G

G

L

V

I
|
I

G

G

C

L

E

E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

-

P

-

S

-

E

-

E

E

V

T

M

Q

P

P

G

R

L

L

L

M

P

S

E

R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A

G

V

I

G

G

L

V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

binding flavin-adenine dinucleotide: G13 (= G13), A15 (≠ S15), D37 (≠ A37), E38 (≠ D38), R45 (≠ K45), P46 (= P46), K50 (≠ T50), A79 (≠ V83), T106 (≠ W109), G107 (= G110), R127 (≠ N130), I153 (= I156), G269 (= G276), D270 (= D277)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 286, 287, 288, 291, 317

2yvjA Crystal structure of the ferredoxin-ferredoxin reductase (bpha3- bpha4)complex (see paper)
33% identity, 71% coverage: 4:277/384 of query aligns to 3:269/402 of 2yvjA

query
sites
2yvjA

Q

K

A

A

P

P

I

V

V

L

G
|
G

T

A

G
|
G

L
|
L

S

A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T
x
G

A
x
D

D
x
E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T

K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M
x
V

G
|
G

A
x
G

G
|
G

L

V

I
|
I

G

G

C

L

E

E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I
x
V

A

-

P

-

S

-

E

-

E
|
E

V
x
T

M

Q

P

P

G

R

L

L

L

M

P

S

E
x
R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L
x
V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L13 (= L14), R44 (≠ K45), P45 (= P46)
binding flavin-adenine dinucleotide: G10 (= G11), G12 (= G13), G35 (≠ T36), D36 (≠ A37), E37 (≠ D38), R44 (≠ K45), P45 (= P46), A78 (≠ V83), T105 (≠ W109), G106 (= G110), R126 (≠ N130), G268 (= G276), D269 (= D277)
binding 1,4-dihydronicotinamide adenine dinucleotide: V147 (≠ M151), G148 (= G152), G149 (≠ A153), G150 (= G154), I152 (= I156), V170 (≠ I174), E171 (= E179), T172 (≠ V180), R179 (≠ E187), G230 (≠ A238), I231 (≠ V239), G232 (= G240), V233 (≠ L241)

Sites not aligning to the query:

active site: 291
binding flavin-adenine dinucleotide: 286, 287, 290, 316
binding 1,4-dihydronicotinamide adenine dinucleotide: 285

2gr2A Crystal structure of ferredoxin reductase, bpha4 (oxidized form)
33% identity, 71% coverage: 4:277/384 of query aligns to 2:268/401 of 2gr2A

query
sites
2gr2A

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G
|
G

L
|
L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T

G

A
x
D

D
x
E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T
x
K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I
x
R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M
x
V

G
|
G

A

G

G
|
G

L
x
V

I
|
I

G

G

C

L

E

E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

-

P

-

S

-

E

-

E
|
E

V
x
T

M

Q

P

P

G

R

L

L

L

M

P

S

E
x
R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L

V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L12 (= L14), R43 (≠ K45), P44 (= P46)
binding adenosine-5-diphosphoribose: R109 (≠ I114), V146 (≠ M151), G147 (= G152), G149 (= G154), V150 (≠ L155), I151 (= I156), E170 (= E179), T171 (≠ V180), R178 (≠ E187), G229 (≠ A238), I230 (≠ V239), G231 (= G240)
binding flavin-adenine dinucleotide: G11 (= G13), A13 (≠ S15), D35 (≠ A37), E36 (≠ D38), R43 (≠ K45), P44 (= P46), K48 (≠ T50), A77 (≠ V83), T104 (≠ W109), G105 (= G110), R125 (≠ N130), G267 (= G276), D268 (= D277)

Sites not aligning to the query:

active site: 290
binding adenosine-5-diphosphoribose: 284
binding flavin-adenine dinucleotide: 285, 286, 289, 315

2gr0A Crystal structure of ferredoxin reductase, bpha4 (oxidized form, NAD+ complex) (see paper)
33% identity, 71% coverage: 4:277/384 of query aligns to 2:268/401 of 2gr0A

query
sites
2gr0A

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G
|
G

L
|
L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T

G

A
x
D

D
x
E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T
x
K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T
|
T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M
x
V

G
|
G

A

G

G
|
G

L

V

I
|
I

G

G

C

L

E

E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

-

P

-

S

-

E

-

E
|
E

V
x
T

M

Q

P

P

G

R

L

L

L

M

P

S

E
x
R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L

V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L12 (= L14), R43 (≠ K45), P44 (= P46)
binding adenosine-5'-diphosphate: V146 (≠ M151), G147 (= G152), G149 (= G154), I151 (= I156), E170 (= E179), T171 (≠ V180), R178 (≠ E187), G229 (≠ A238), I230 (≠ V239), G231 (= G240)
binding flavin-adenine dinucleotide: G11 (= G13), A13 (≠ S15), D35 (≠ A37), E36 (≠ D38), R43 (≠ K45), P44 (= P46), K48 (≠ T50), T76 (= T82), A77 (≠ V83), T104 (≠ W109), G105 (= G110), R125 (≠ N130), I151 (= I156), G267 (= G276), D268 (= D277)

Sites not aligning to the query:

active site: 290
binding flavin-adenine dinucleotide: 284, 285, 286, 289, 315

4h4uA Crystal structure of ferredoxin reductase, bpha4 t176r mutant (reduced form)
33% identity, 71% coverage: 4:277/384 of query aligns to 3:269/401 of 4h4uA

query
sites
4h4uA

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G
|
G

L
|
L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T

G

A
x
D

D

E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T

K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I
x
L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M

V

G

G

A

G

G

G

L
x
V

I
|
I

G

G

C

L

E
|
E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

-

P

-

S

-

E

-

E
|
E

V
x
R

M
x
Q

P

P

G

R

L

L

L

M

P

S

E

R

A

A

A

-

H

-

A

-

V

-

Q

P

Q

A

E

T

L

L

E

A

N

D

L

F

G

V

V

A

G

R

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L

V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L13 (= L14), R44 (≠ K45), P45 (= P46)
binding flavin-adenine dinucleotide: G12 (= G13), A14 (≠ S15), D36 (≠ A37), R44 (≠ K45), P45 (= P46), A78 (≠ V83), T105 (≠ W109), G106 (= G110), L125 (≠ I129), R126 (≠ N130), I152 (= I156), E155 (= E159), G268 (= G276), D269 (= D277)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V151 (≠ L155), I152 (= I156), E171 (= E179), R172 (≠ V180), Q173 (≠ M181), G230 (≠ A238), I231 (≠ V239), G232 (= G240)

Sites not aligning to the query:

active site: 291
binding flavin-adenine dinucleotide: 285, 286, 287, 290, 316
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 284, 285, 315

8c0zE Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
27% identity, 71% coverage: 8:280/384 of query aligns to 4:279/424 of 8c0zE

query
sites
8c0zE

I

V

I

I

V

L

G
|
G

T

N

G

G

L
x
P

S

A

G

G

Y

V

S

I

L

A

A

A

R

E

E

T

I

L

R

R

K

R

Q

A

D

A

K

P

E

T

T

D

P

D

V

I

L

L

I

L

V

F

T

G

A

S

D

E

D

D

G

A

V

P

S

P

Y

Y

S

S

K
x
R

P
x
M

M

A

L

I

S
x
P

T

Y

G

L

F

L

T

E

K

G

G

N

K

I

D

D

A

E

D

S

G

G

L

T

A

W

Q

L

G

R

G

K

T

S

D

P

A

G

M

H

A

F

D

D

Q

R

L

L

N

R

V

I

R

H

I

E

R

M

T

R

F

G

V

R

T

A

V

V

T

S

G

-

I

L

D

D

T

S

E

E

A

R

H

R

E

R

L

I

I

L

L

F

G

D

D

D

E

G

R

H

L

F

-

E

A

S

Y

W

S

D

K

R

L

L

V

L

L

I

A

A

W
x
T

G

G

A

S

D

H

V

P

I

V

R

R

L

P

G

P

L

I

D

P

G

G

D

I

G

D

Q

L

E

P

H

E

V

V

F

Q

S

T

I

C

N
x
W

D

T

L

L

M

E

D

D

Y

A

R

R

A

A

F

I

R

A

D

R

A

F

L

A

G

T

D

P

G

G

K

A

R

R

V

V

A

L

I

Q

M

L

G

G

A

A

G

G

L
x
F

I

I

G

G

C

C

E

I

F

I

A

M

N

E

D

A

L

L

R

A

N

A

G

R

G

G

Y

V

E

E

V

L

D

T

V

V

I

V

A

E

P

M

S

G

E

D

E

R

V

M

M

V

P

P

G

R

L

M

P

T

E

P

A

T

A

A

A

G

H

G

A

M

V

I

Q

R

Q

K

E

W

L

V

E

E

N

D

L

Q

G

G

V

V

G

R

F

V

H

V

L

T

G

N

T

A

V

G

V

V

D

S

R

R

I

I

D

D

R

C

R

R

G

A

D

S

G

N

-

D

-

A

-

P

V

L

S

D

L

V

T

T

L

L

A

S

N

T

G

G

E

E

T

V

L

V

D

V

A

A

D

D

L

L

V

V

L

I

S

V

A

A

V

A

G

G

L

V

R

A

P

P

R

N

T

I

D

A

L

F

A

L

R

E

A

A

A

T

G

P

L

V

E

H

T

V

A

A

R

K

G

G

I

V

V

L

V

V

N

D

R

D

A

R

L

L

E

Q

T

T

S

S

A

V

P

P

D

G

V

I

Y

F

A

A

L

A

G

G

D
|
D

C

V

A

A

E

E

binding flavin-adenine dinucleotide: G7 (= G11), P10 (≠ L14), R41 (≠ K45), M42 (≠ P46), P45 (≠ S49), T105 (≠ W109), W126 (≠ N130), F151 (≠ L155), D276 (= D277)

8a56B Coenzyme a-persulfide reductase (coapr) from enterococcus faecalis (see paper)
29% identity, 73% coverage: 7:285/384 of query aligns to 3:287/539 of 8a56B

query
sites
8a56B

I

I

I

V

I

I

V
x
I

G
|
G

T

G

G
x
V

L

A

S
x
G

G

G

Y

M

S

S

L

A

A

A

R
x
T

E
x
R

I

L

R

R

K
x
R

Q

L

D

M

K

E

E

D

T

A

P

E

V

I

L

V

I

V

V

M

T
x
E

A
x
K

D

G

D

P

G

F

V

V

S

S

Y

F

S

A

K

N

-
x
C

-

G

-

L

P

P

M

Y

L

Y

S

V

T

S

G

G

F

-

T

-

K

E

G

I

K

A

D

E

A

R

D

E

G

Q

L

L

A

L

Q

V

G

Q

G

T

T

P

D

E

A

A

M

L

A

K

D

A

Q

R

L
x
F

N

N

V

L

R

D

I

V

R

R

T

P

F

H

V

H

T

E

V
|
V

T

V

G

A

I

I

D

D

-

P

-

I

-

E

-

K

-

V

-

I

T

T

E

V

A

K

H

H

E

E

L

T

I

E

L

I

G

L

D

T

E

E

R

H

L

-

A

-

Y

Y

S

D

K

K

L

L

V

I

L

L

A
x
S

W
x
P

G
|
G

A

A

D

K

V

P

I

F

R

V

L

P

G

P

L

I

D

T

G

G

D

L

G

A

Q

E

-

A

E

K

H

N

V

V

F

F

S

S

I

L

N

R

D

N

L

V

M

P

D

D

Y

L

R

D

A

Q

F

I

R

M

D

T

A

A

L

L

G

T

-

P

D

E

G

T

K

K

R

R

V

A

A

V

I

V

M

I

G

G

A

A

G

G

L

F

I
|
I

G

G

C

L

E

E

F

M

A

A

N

E

D

N

L

L

R

Q

N

K

G

R

G

G

Y

L

E

E

V

V

D

T

V

L

I

V

A

E

P

K

S

A

E

P

E

H

V

V

M

L

P

P

G

P

L

L

L

D

P

E

E

E

A

M

A

A

H

F

A

-

V

V

Q

K

Q

A

E

E

L

L

E

S

N

K

L

N

G

N

V

V

G

Q

F

V

H

I

L

T

G

G

T

Q

V

S

V

A

D

V

R

A

I

F

D

E

R

E

G

G

D

Q

G

V

V

I

S

R

L

L

T

E

L

-

A

-

N

D

G

G

E

Q

T

T

L

L

D

A

A

S

D

D

L

L

V

T

L

I

S

L

A

S

V

V

G

G

L

V

R

Q

P

P

R

E

T

N

D

T

L

L

A

A

R

V

A

E

A

A

G

G

L

V

E

A

T

T

A

G

R

L

-

R

-

G

G

G

I

I

V

V

N

D

R

E

A

H

L

Y

E

Q

T

T

S

N

A

Q

P

P

D

D

V

I

Y

Y

A

A

L

V

G
|
G

D
|
D

C

A

A

I

E

V

V

V

D

K

G

Q

H

Q

V

I

binding flavin-adenine dinucleotide: I6 (≠ V10), G7 (= G11), V9 (≠ G13), G11 (≠ S15), E32 (≠ T36), K33 (≠ A37), C42 (vs. gap), V80 (= V83), S109 (≠ A108), P110 (≠ W109), G111 (= G110), I159 (= I156), G278 (= G276), D279 (= D277)
binding 3'-phosphate-adenosine-5'-diphosphate: T17 (≠ R21), R18 (≠ E22), R21 (≠ K25), F70 (≠ L73)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 295, 296, 297, 422, 423
binding 3'-phosphate-adenosine-5'-diphosphate: 305, 356, 430, 435, 438, 505, 506, 509

4h4wA Crystal structure of ferredoxin reductase, bpha4 e175c/t176r/q177g mutant (reduced form)
32% identity, 71% coverage: 4:277/384 of query aligns to 2:268/399 of 4h4wA

query
sites
4h4wA

Q

K

A

A

P

P

I

V

V

L

G

G

T

A

G
|
G

L
|
L

S
x
A

G

S

Y

V

S

S

L

F

A

V

R

A

E

E

I

L

R

R

K

Q

Q

A

D

G

K

Y

E

Q

T

G

P

L

V

I

L

T

I

V

V

V

T

G

A
x
D

D

E

D

A

G

E

V

R

S

P

Y

Y

S

D

K
x
R

P
|
P

M

P

L

L

S

S

T

K

G

D

F

F

T

M

K

A

G

H

K

G

D

D

A

A

D

E

G

K

L

I

A

R

Q

L

G

D

G

C

T

K

D

R

A

A

M

P

A

-

D

-

Q

-

L

-

N

E

V

V

R

E

I

W

R

L

T

L

F

G

V

V

T

T

V
x
A

T

Q

G

S

I

F

D

D

T

P

E

Q

A

A

H

H

E

T

L

V

I

A

L

L

G

S

D

D

E

G

R

R

-

T

L

L

A

P

Y

Y

S

G

K

T

L

L

V

V

L

L

A

A

W
x
T

G
|
G

A

A

D

A

V

P

I

R

R

A

L

L

G

P

L

T

D

L

G

Q

D

G

G

A

Q

T

E

M

H

P

V

V

F

H

S

T

I

L

N
x
R

D

T

L

L

M

E

D

D

Y

A

R

R

A

R

F

I

R

Q

D

A

A

G

L

L

G

R

D

P

G

Q

K

S

R

R

V

L

A

L

I

I

M

V

G

G

A
x
G

G

G

L

V

I
|
I

G

G

C

L

E
|
E

F

L

A

A

N

A

D

T

L

A

R

R

N

T

G

A

G

G

Y

V

E

H

V

V

D

S

V

L

I

V

A

C

P
x
R

S

G

E

P

E

R

V

L

M

M

P
x
S

G
x
R

L

A

P

P

E

A

A

T

A

L

A

A

H

D

A

F

V

V

Q

A

Q

R

E

-

L

-

E

-

N

-

L

-

G

-

V

-

G

-

F

Y

H

H

L

A

G

A

T

Q

V

G

V

V

D

D

-

L

R

R

I

F

D

E

R

R

R

S

G

V

-

T

-

G

-

S

-

V

D

D

G

G

V

V

S

V

L

L

T

-

L

L

A

D

N

D

G

G

E

T

T

R

L

I

D

A

A

A

D

D

L

M

V

V

L

V

S

V

A
x
G

V
x
I

G
|
G

L

V

R

L

P

A

R

N

T

D

D

A

L

L

A

A

R

R

A

A

G

G

L

L

E

A

T

C

A

D

R

D

G

G

I

I

V

F

V

V

N

D

R

A

A

Y

L

G

E

R

T

T

S

T

A

C

P

P

D

D

V

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L12 (= L14), R43 (≠ K45), P44 (= P46)
binding flavin-adenine dinucleotide: G11 (= G13), A13 (≠ S15), D35 (≠ A37), R43 (≠ K45), P44 (= P46), A77 (≠ V83), T104 (≠ W109), G105 (= G110), R125 (≠ N130), I151 (= I156), E154 (= E159), G267 (= G276), D268 (= D277)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G148 (≠ A153), I151 (= I156), R171 (≠ P176), S177 (≠ P182), R178 (≠ G183), G229 (≠ A238), I230 (≠ V239), G231 (= G240)

Sites not aligning to the query:

active site: 290
binding flavin-adenine dinucleotide: 285, 286, 289, 315

4emiA Toluene dioxygenase reductase in reduced state in complex with NAD+ (see paper)
28% identity, 71% coverage: 7:280/384 of query aligns to 4:277/402 of 4emiA

query
sites
4emiA

I

V

I

A

I

I

V

I

G

G

T

N

G
|
G

L
x
V

S
x
G

G

G

Y

F

S

T

L

T

A

A

R

Q

E

A

I

L

R

R

K

A

Q

E

D

G

K

F

E

E

T

G

P

R

V

I

L

S

I

L

V

I

T

G

A
x
D

D
x
E

D

P

G

H

V

L

S

P

Y

Y

S

D

K
x
R

P
|
P

M

S

L

L

S

S

T
x
K

G

A

F

V

T

L

K

D

G

G

K

S

D

-

A

-

D

-

G

-

L

L

A

E

Q

R

G

P

T

I

D

L

A

A

M

E

A

A

D

D

-

W

-

Y

-

G

Q

E

L

A

N

R

V

I

R

D

I

M

R

L

T

T

F

G

V

P

T
x
E

V
|
V

T

T

G

A

I

L

D

D

T

V

E

Q

A

T

H

R

E

T

L

I

I

S

L

L

G

D

D

D

-

G

E

T

R

T

L

L

A

S

Y

A

S

D

K

A

L

I

V

V

L

I

A

A

W
x
T

G
|
G

A

S

D

R

V

A

I
x
R

R

T

L

M

G

A

L

L

D

P

G

G

D

S

G

Q

Q

L

E

P

H

G

V

V

F

V

S

T

I

L

N

R

D

T

L

Y

M

G

D

D

Y

V

R

Q

A

V

F

L

R

R

D

D

A

S

L

W

G

T

D

S

G

A

K

T

R

R

V

L

A

L

I

I

M

V

G
|
G

A

G

G

G

L
|
L

I
|
I

G

G

C

C

E
|
E

F

V

A

A

N

T

D

T

L

A

R

R

N

K

G

L

G

G

Y

L

E

S

V

V

D

T

V

I

I

L

A
x
E

P
x
A

S

G

E

D

E

E

V

L

M

L

P

V

G
x
R

L

V

L

L

P

G

E

R

A

R

A

I

A

G

H

A

A

W

V

L

Q

R

Q

G

E

L

L

L

E

T

N

E

L

L

G

G

V

V

G

Q

F

V

H

E

L

L

G

G

T

T

V

G

V

V

D

V

R

G

I

F

D

S

R

G

R

E

G

G

D

Q

G

L

V

E

S

Q

L

V

T

M

L

A

A

S

N

D

G

G

E

R

T

S

L

F

D

V

A

A

D

D

L

S

V

A

L

L

S

I

A

C

V
|
V

G
|
G

L
x
A

R

E

P

P

R

A

T

D

D

Q

L

L

A

A

R

R

A

Q

A

A

G

G

L

L

E

A

T

C

A

D

R

R

G

G

I

V

V

I

V

V

N

D

R

H

A

C

L

G

E

A

T

T

S

L

A

A

P

K

D

G

V

V

Y

F

A

A

L

V

G
|
G

D
|
D

C

V

A

A

E

S

binding flavin-adenine dinucleotide: G10 (= G13), V11 (≠ L14), G12 (≠ S15), D34 (≠ A37), E35 (≠ D38), R42 (≠ K45), P43 (= P46), K47 (≠ T50), E78 (≠ T82), V79 (= V83), T106 (≠ W109), G107 (= G110), G273 (= G276), D274 (= D277)
binding nicotinamide-adenine-dinucleotide: R111 (≠ I114), G149 (= G152), L152 (= L155), I153 (= I156), E156 (= E159), E172 (≠ A175), A173 (≠ P176), R180 (≠ G183), V236 (= V239), G237 (= G240), A238 (≠ L241)

Sites not aligning to the query:

4emjA Complex between the reductase and ferredoxin components of toluene dioxygenase (see paper)
29% identity, 71% coverage: 7:279/384 of query aligns to 5:277/406 of 4emjA

query
sites
4emjA

I

V

I

A

I

I

V

I

G

G

T

N

G
|
G

L
x
V

S
x
G

G

G

Y

F

S

T

L

T

A

A

R

Q

E

A

I

L

R

R

K

A

Q

E

D

G

K

F

E

E

T

G

P

R

V

I

L

S

I

L

V

I

T

G

A
x
D

D
x
E

D

P

G

H

V

L

S

P

Y

Y

S

D

K
x
R

P
|
P

M

S

L

L

S
|
S

T
x
K

G

A

F

V

T

L

K

D

G

G

K

S

D

-

A

-

D

-

G

-

L

L

A

E

Q

R

G

P

T

I

D

L

A

A

M

E

A

A

D

D

-

W

-

Y

-

G

Q

E

L

A

N

R

V

I

R

D

I

M

R

L

T

T

F

G

V

P

T

E

V
|
V

T

T

G

A

I

L

D

D

T

V

E

Q

A

T

H

R

E

T

L

I

I

S

L

L

G

D

D

D

-

G

E

T

R

T

L

L

A

S

Y

A

S

D

K

A

L

I

V

V

L

I

A

A

W
x
T

G
|
G

A

S

D

R

V

A

I

R

R

T

L

M

G

A

L

L

D

P

G

G

D

S

G

Q

Q

L

E

P

H

G

V

V

F

V

S

T

I

L

N
x
R

D

T

L

Y

M

G

D

D

Y

V

R

Q

A

V

F

L

R

R

D

D

A

S

L

W

G

T

D

S

G

A

K

T

R

R

V

L

A

L

I

I

M

V

G

G

A

G

G

G

L

L

I

I

G

G

C

C

E

E

F

V

A

A

N

T

D

T

L

A

R

R

N

K

G

L

G

G

Y

L

E

S

V

V

D

T

V

I

I

L

A

E

P

A

S

G

E

D

E

E

V

L

M

L

P

V

G

R

L

V

L

L

P

G

E

R

A

R

A

I

A

G

H

A

A

W

V

L

Q

R

Q

G

E

L

L

L

E

T

N

E

L

L

G

G

V

V

G

Q

F

V

H

E

L

L

G

G

T

T

V

G

V

V

D

V

R

G

I

F

D

S

R

G

R

E

G

G

D

Q

G

L

V

E

S

Q

L

V

T

M

L

A

A

S

N

D

G

G

E

R

T

S

L

F

D

V

A

A

D

D

L

S

V

A

L

L

S

I

A

C

V

V

G

G

L

A

R

E

P

P

R

A

T

D

D

Q

L

L

A

A

R

R

A

Q

A

A

G

G

L

L

E

A

T

C

A

D

R

R

G

G

I

V

V

I

V

V

N

D

R

H

A

C

L

G

E

A

T

T

S

L

A

A

P

K

D

G

V

V

Y

F

A

A

L

V

G
|
G

D
|
D

C

V

A

A

binding flavin-adenine dinucleotide: G11 (= G13), V12 (≠ L14), G13 (≠ S15), D35 (≠ A37), E36 (≠ D38), R43 (≠ K45), P44 (= P46), S47 (= S49), K48 (≠ T50), V80 (= V83), T107 (≠ W109), G108 (= G110), R128 (≠ N130), G274 (= G276), D275 (= D277)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 291, 292, 319, 320

6pfzA Structure of a NAD-dependent persulfide reductase from a. Fulgidus (see paper)
29% identity, 67% coverage: 23:280/384 of query aligns to 19:290/541 of 6pfzA

query
sites
6pfzA

I

I

R

R

K
x
R

Q

K

D

D

K

G

E

D

T

A

P

S

V

I

L

T

I

V

V

V

T
x
E

A
|
A

D

G

D

K

G

Y

V
|
V

S
|
S

Y

L

S
x
G

K
x
R

-
x
C

-

G

-

L

P

P

M

Y

L

Y

S

V

T

G

G

G

F

L

T

V

K

H

G

-

K

-

D

E

A

V

D

D

G

N

L

L

A

R

Q

E

G

T

T

Y

D

G

A

A

M

V

A

R

D

D

Q

E

L

A

-

Y

-

F

-

K

-

L

-
x
K

N

N

V

I

R

D

I

V

R

L

T

T

F

E

V

T

T

V

V
x
A

T

T

G

E

I

I

D

D

T

R

E

S

A

R

H

K

E

T

L

V

I

K

L

I

-

V

-

R

-

N

-

G

G

S

D

E

E

D

R

E

L

L

A

N

Y

Y

S

D

K

Y

L

L

V

V

L

I

A
|
A

W
x
T

G
|
G

A

A

D

R

V

P

I

A

R

K

L

P

G

P

L

I

D

E

G

G

D

I

G

E

Q

A

E

E

H

G

V

V

F

V

S

T

I
x
L

N

T

D

S

L

A

M

E

D

E

Y

A

R

E

A

K

F

I

R

I

D

E

A

M

L

W

G

E

D

E

G

G

-

A

K

E

R

K

V

A

A

V

I

V

M

I

G

G

A

A

G

G

L

F

I

I

G

G

C

L

E

E

F

S

A

A

N

E

D

A

L

L

R

K

N

N

G

L

G

D

Y

M

E

E

V

V

D

T

V

V

I

I

A

E

P

M

S

M

E

D

E

R

V

V

M

A

P

P

G

A

L

M

L

L

P

D

E

R

A

E

A

M

A

A

H

V

A

L

V

V

Q

E

Q

N

E

H

L

L

E

R

N

E

L

K

G

G

V

V

G

N

F

V

H

V

L

T

G

S

T

T

V

R

V

V

D

E

R

K

I

I

D

V

R

S

R

Q

G

D

D

D

G

K

V

V

S

R

L

A

T

V

L

I

A

A

N

N

G

G

E

K

T

E

L

Y

D

P

A

A

D

D

L

V

V

V

L

V

S

V

A

A

V

T

G

G

L

I

R

K

P

P

R

N

T

S

D

E

L

L

A

A

R

E

A

K

A

A

G

G

L

L

E

K

T

I

A

G

R

E

-

T

-

G

G

A

I

I

V

W

V

V

N

D

R

E

A

Y

L

M

E

R

T

T

S

S

A

D

P

E

D

S

V

I

Y

Y

A

A

L

G

G
|
G

D
|
D

C

C

A

V

E

E

active site: V37 (= V41), C42 (vs. gap)
binding coenzyme a: R21 (≠ K25), S38 (= S42), G40 (≠ S44), C42 (vs. gap), K76 (vs. gap)
binding flavin-adenine dinucleotide: E32 (≠ T36), A33 (= A37), R41 (≠ K45), C42 (vs. gap), A86 (≠ V83), A115 (= A108), T116 (≠ W109), G117 (= G110), L136 (≠ I129), G286 (= G276), D287 (= D277)

Sites not aligning to the query:

active site: 455, 456
binding calcium ion: 306, 332, 427, 428
binding coenzyme a: 14, 18, 313, 430, 447, 512, 513, 516
binding flavin-adenine dinucleotide: 7, 11, 303, 304, 305

Query Sequence

>GFF3407 FitnessBrowser__Marino:GFF3407
MTEQAPIIIVGTGLSGYSLAREIRKQDKETPVLIVTADDGVSYSKPMLSTGFTKGKDADG
LAQGGTDAMADQLNVRIRTFVTVTGIDTEAHELILGDERLAYSKLVLAWGADVIRLGLDG
DGQEHVFSINDLMDYRAFRDALGDGKRVAIMGAGLIGCEFANDLRNGGYEVDVIAPSEEV
MPGLLPEAAAHAVQQELENLGVGFHLGTVVDRIDRRGDGVSLTLANGETLDADLVLSAVG
LRPRTDLARAAGLETARGIVVNRALETSAPDVYALGDCAEVDGHVLLYVLPLMACSRALA
KTLLGERTEVSYGTMPVMIKTPCCPTAVCPPPSEAEGNWEIEQDGTSVKALFKSAGGDVL
GFAVTGSYAMEKQALSKEVPPIHG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory