Comparing GFF3417 FitnessBrowser__Phaeo:GFF3417 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 96% coverage: 1:265/276 of query aligns to 37:297/307 of Q94JV5
Sites not aligning to the query:
Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 2 papers)
34% identity, 98% coverage: 1:271/276 of query aligns to 4:265/276 of Q9NQR4
P47016 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 98% coverage: 2:272/276 of query aligns to 7:303/307 of P47016
4hg5A Structural insights into yeast nit2: wild-type yeast nit2 in complex with oxaloacetate (see paper)
32% identity, 98% coverage: 2:272/276 of query aligns to 4:300/304 of 4hg5A
4hg3A Structural insights into yeast nit2: wild-type yeast nit2 in complex with alpha-ketoglutarate (see paper)
32% identity, 98% coverage: 2:272/276 of query aligns to 4:300/304 of 4hg3A
4hgdA Structural insights into yeast nit2: c169s mutant of yeast nit2 in complex with an endogenous peptide-like ligand (see paper)
32% identity, 98% coverage: 2:272/276 of query aligns to 3:296/299 of 4hgdA
3klcB Crystal structure of hyperthermophilic nitrilase (see paper)
27% identity, 92% coverage: 1:254/276 of query aligns to 1:238/261 of 3klcB
Sites not aligning to the query:
3klcA Crystal structure of hyperthermophilic nitrilase (see paper)
27% identity, 92% coverage: 1:254/276 of query aligns to 1:238/261 of 3klcA
Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
27% identity, 95% coverage: 1:263/276 of query aligns to 2:257/262 of Q9UYV8
7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide (see paper)
28% identity, 92% coverage: 1:254/276 of query aligns to 10:240/263 of 7ovgA
6ypaB The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
28% identity, 92% coverage: 1:254/276 of query aligns to 16:246/269 of 6ypaB
Q44185 N-carbamoyl-D-amino acid hydrolase; D-N-alpha-carbamilase; EC 3.5.1.77 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter) (see paper)
26% identity, 92% coverage: 18:272/276 of query aligns to 25:294/304 of Q44185
4iztA The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
31% identity, 95% coverage: 1:262/276 of query aligns to 10:255/263 of 4iztA
4izuA The e41q mutant of the amidase from nesterenkonia sp. An1 showing the result of michael addition of acrylamide at the active site cysteine
30% identity, 96% coverage: 1:264/276 of query aligns to 2:249/254 of 4izuA
5nybA A c145a mutant of nesterenkonia an1 amidase bound to adipamide
31% identity, 95% coverage: 1:262/276 of query aligns to 9:254/262 of 5nybA
5ny7A A c145a mutant of nesterenkonia an1 amidase bound to nicotinamide
31% identity, 95% coverage: 1:262/276 of query aligns to 9:254/262 of 5ny7A
Sites not aligning to the query:
8hpcC Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine
26% identity, 90% coverage: 24:272/276 of query aligns to 30:293/303 of 8hpcC
5h8jB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with cadaverine (see paper)
24% identity, 93% coverage: 6:261/276 of query aligns to 10:270/297 of 5h8jB
1uf8A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-phenylalanine
26% identity, 90% coverage: 24:272/276 of query aligns to 30:293/303 of 1uf8A
1uf7A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-valine
26% identity, 90% coverage: 24:272/276 of query aligns to 30:293/303 of 1uf7A
>GFF3417 FitnessBrowser__Phaeo:GFF3417
MRAALLQMTSSDQPAENLGIVRQMIDDAVAGGAGFVLTPEVTNCLSGSRSHQQSVLHQEA
DDPTLSALRDTAAQHGIWLSLGSLALKTTDTDGRFANRQFLIDPKGEIVARYDKIHMFDV
DVTPEETYRESDGYRPGHEAVVAETPFAVLGLTICYDVRFPYLHRRLAKAGAQVLLAPAA
FSHVTGAAHWHALLQARAIETGCYVLAAAQTGTHAASRGQSRRTYGHSLAVSPWGEILAD
GGTDIGITYIDLDLEKVAQARRQVPSLSHDREFDGP
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory