Comparing GFF3430 FitnessBrowser__WCS417:GFF3430 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1qs0B Crystal structure of pseudomonas putida 2-oxoisovalerate dehydrogenase (branched-chain alpha-keto acid dehydrogenase, e1b) (see paper)
92% identity, 96% coverage: 16:352/352 of query aligns to 2:338/338 of 1qs0B
Q5SLR3 2-oxoisovalerate dehydrogenase subunit beta; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta; EC 1.2.4.4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
51% identity, 95% coverage: 19:351/352 of query aligns to 4:323/324 of Q5SLR3
1umdD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methyl-2-oxopentanoate as an intermediate (see paper)
51% identity, 95% coverage: 19:351/352 of query aligns to 3:322/323 of 1umdD
1umcD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methylpentanoate (see paper)
51% identity, 95% coverage: 19:351/352 of query aligns to 3:322/323 of 1umcD
1umbD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 in holo-form (see paper)
51% identity, 95% coverage: 19:351/352 of query aligns to 3:322/323 of 1umbD
3dv0D Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
47% identity, 94% coverage: 19:350/352 of query aligns to 3:322/324 of 3dv0D
3dv0B Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
47% identity, 94% coverage: 19:350/352 of query aligns to 3:322/324 of 3dv0B
3dufD Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
47% identity, 94% coverage: 19:350/352 of query aligns to 3:322/324 of 3dufD
1w85B The crystal structure of pyruvate dehydrogenase e1 bound to the peripheral subunit binding domain of e2 (see paper)
47% identity, 94% coverage: 19:350/352 of query aligns to 3:322/324 of 1w85B
1dtwB Human branched-chain alpha-keto acid dehydrogenase (see paper)
45% identity, 95% coverage: 16:351/352 of query aligns to 2:325/326 of 1dtwB
2j9fD Human branched-chain alpha-ketoacid dehydrogenase-decarboxylase e1b (see paper)
45% identity, 95% coverage: 16:351/352 of query aligns to 5:328/329 of 2j9fD
P21953 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; EC 1.2.4.4 from Homo sapiens (Human) (see 2 papers)
45% identity, 95% coverage: 16:351/352 of query aligns to 68:391/392 of P21953
P11177 Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-B; EC 1.2.4.1 from Homo sapiens (Human) (see 6 papers)
36% identity, 91% coverage: 12:331/352 of query aligns to 26:336/359 of P11177
Sites not aligning to the query:
6cfoB Human pyruvate dehydrogenase e1 component complex with covalent tdp adduct acetyl phosphinate (see paper)
35% identity, 96% coverage: 16:352/352 of query aligns to 1:330/330 of 6cfoB
6cerD Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
35% identity, 96% coverage: 16:352/352 of query aligns to 2:331/331 of 6cerD
6yakDDD C-terminal component of the split chain transketolase (see paper)
26% identity, 69% coverage: 67:308/352 of query aligns to 46:265/311 of 6yakDDD
Sites not aligning to the query:
8bzxB 1-deoxy-d-xylulose 5-phosphate synthase from klebsiella pneumoniae (kpdxps),co-crystal with thiamine monophosphate analog (see paper)
23% identity, 70% coverage: 61:308/352 of query aligns to 304:524/568 of 8bzxB
Sites not aligning to the query:
8a9cB Structure of truncated 1-deoxy-d-xylulose 5-phosphate synthase (dxs) from klebsiella pneumoniae in complex with cofactor tpp
23% identity, 70% coverage: 61:308/352 of query aligns to 257:477/520 of 8a9cB
Sites not aligning to the query:
P77488 1-deoxy-D-xylulose-5-phosphate synthase; 1-deoxyxylulose-5-phosphate synthase; DXP synthase; DXPS; EC 2.2.1.7 from Escherichia coli (strain K12) (see 2 papers)
23% identity, 69% coverage: 67:308/352 of query aligns to 362:576/620 of P77488
Sites not aligning to the query:
8bzxA 1-deoxy-d-xylulose 5-phosphate synthase from klebsiella pneumoniae (kpdxps),co-crystal with thiamine monophosphate analog (see paper)
23% identity, 70% coverage: 61:308/352 of query aligns to 282:502/546 of 8bzxA
Sites not aligning to the query:
>GFF3430 FitnessBrowser__WCS417:GFF3430
MNDHNNSIEVETAMTTTTMTMIQALRSAMDVMLERDDNVVVFGQDVGYFGGVFRCTEGLQ
TKYGSSRVFDAPISESGIVGVAVGMGAYGLRPVAEIQFADYVYPATDQIISEAARLRYRS
AGQFTAPLTMRMPCGGGIYGGQTHSQSIEAVFTQVCGLRTVMPSNPYDAKGLLIASIEND
DPVIFLEPKRLYNGPFDGHHDRPVTPWSKHPQAQVPDGYYTVPLDVAAIVRPGSAVTVLT
YGTTVYVSQVAAEETGIDAEVIDLRSLWPLDLETIVKSVKKTGRCVVVHEATRTCGFGAE
LVSLVQEHCFHHLEAPIERVTGWDTPYPHAQEWAYFPGPSRVGAALKRVMEV
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory